VIM cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin)

Gene
Clones
gRNAs

Summary

Gene Symbol	VIM
Entrez Gene ID	108498453
Full Name	vimentin
Gene Type	protein-coding
Organism	Lepidothrix coronata(blue-crowned manakin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017817208.1	XP_017672697.1	vimentin

Pathways from BioSystems
Gene Ontology

The following VIM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VIM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLe59909	XM_017817208.1 Latest version!	Lepidothrix coronata vimentin (VIM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VIM ( XM_017817208.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017672697.1 Lepidothrix coronata vimentin (VIM), mRNA.

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CloneID	OLe59909
Clone ID Related Accession (Same CDS sequence)	XM_017817208.1
Accession Version	XM_017817208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1380bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	vimentin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690239.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCA GCACCAAGAA CTCCTCGTAC CGCCGCATGT TCGGCGGGGG CAGCCGCCCC 
AGCACCGGCA CCCGCTACAT CACGTCGAGC AGCCGGTACT CGCTGGGCAG CGCCCTGCGG 
CCCAGCACGG CCCGGTACGT GTCCGCCTCG CCCGGCGGCA TGTACACCAG CAAGACGACG 
TCGGTGCGGC TGCGGAGCAG CATGCCGCCC ATGCGGCTCC ACGACTCCGT GGACTTCTCC 
CTGGCCGACG CCATCAACAC GGAGTTCAAG GCGAACCGCA CCAACGAGAA GGTTGAGCTG 
CAGGAGCTAA ATGACCGCTT CGCTAACTAC ATCGACAAGG TGCGCTTCCT GGAGCAGCAG 
AACAAGATTC TGCTGGCCGA GCTGGAGCAG CTCAAGGGCA AGGGCACGTC CCGCCTGGGC 
GACCTCTACG AGGAGGAGAT GCGGGAGCTG CGGCGGCAGG TGGACCAGCT CACCAACGAT 
AAGGCACGCG TGGAAGTGGA GCGGGACAAC CTCGCCGACG ACATCATGCG GCTGAGGGAG 
AAGTTGCAAG AAGAGATGCT TCAGAGGGAG GAGGCCGAGA ACACCCTGCA GTCCTTCAGA 
CAGGATGTTG ACAATGCCTC TCTGGCACGT CTTGATCTTG AGCGCAAAGT TGAGTCCCTG 
CAAGAGGAGA TTGTCTTCTT GAAGAAGCTT CATGATGAGG AAATCCGAGA ATTGCAGGCC 
CAACTCCAGG AACAGCACAT CCAAATCGAT ATGGATGTTT CTAAGCCTGA TCTCACTGCT 
GCCCTGCGTG ATGTTCGCCA ACAGTATGAA AGCGTTGCTG CTAAGAATCT TCAGGAAGCT 
GAAGAGTGGT ACAAGTCCAA ATTTGCAGAT CTCTCTGAAG CTGCTAATAG GAACAACGAT 
GCCCTGCGCC AGGCCAAACA AGAAGCCAAT GAGTACCGCA GACAGATCCA GTCTCTCACC 
TGCGAAGTTG ATGCTCTTAA GGGAAGCAAT GAGTCCCTGG AGCGCCAAAT GCGTGAAATG 
GAGGAGAATT TTGCTATGGA AGCTGCTAAC TACCAGGACA CTATTGGCCG TCTGCAGGAT 
GAGATTCAGA ACATGAAGGA AGAAATGGCT CGCCATCTGC GTGAGTACCA GGACCTGCTG 
AATGTAAAGA TGGCTCTTGA TATTGAGATT GCCACCTACA GAAAGCTGCT GGAGGGTGAA 
GAGAGCAGGA TTAACATGCC CATTCCAACC TTTGCTTCTT TGAACCTGAG AGAAACCAAC 
ATTGAGTCTC AGCCAATGGT TGACACTCAC TCAAAGAGGA CACTTCTAAT TAAGACCGTC 
GAAACTAGAG ATGGACAGGT TATTAATGAA ACTTCCCAGC ATCATGATGA CTTGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017672697.1
CDS	98..1477
Translation	MSSSTKNSSYRRMFGGGSRPSTGTRYITSSSRYSLGSALRPSTARYVSASPGGMYTSKTTSVRLRSSMPPMRLHDSVDFSLADAINTEFKANRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGKGTSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLADDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIVFLKKLHDEEIRELQAQLQEQHIQIDMDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQEANEYRRQIQSLTCEVDALKGSNESLERQMREMEENFAMEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRINMPIPTFASLNLRETNIESQPMVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE

Target ORF information:

RefSeq Version	XM_017817208.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata vimentin (VIM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017817208.1

ORF Insert Sequence:

ATGAGCAGCA GCACCAAGAA CTCCTCGTAC CGCCGCATGT TCGGCGGGGG CAGCCGCCCC 
AGCACCGGCA CCCGCTACAT CACGTCGAGC AGCCGGTACT CGCTGGGCAG CGCCCTGCGG 
CCCAGCACGG CCCGGTACGT GTCCGCCTCG CCCGGCGGCA TGTACACCAG CAAGACGACG 
TCGGTGCGGC TGCGGAGCAG CATGCCGCCC ATGCGGCTCC ACGACTCCGT GGACTTCTCC 
CTGGCCGACG CCATCAACAC GGAGTTCAAG GCGAACCGCA CCAACGAGAA GGTTGAGCTG 
CAGGAGCTAA ATGACCGCTT CGCTAACTAC ATCGACAAGG TGCGCTTCCT GGAGCAGCAG 
AACAAGATTC TGCTGGCCGA GCTGGAGCAG CTCAAGGGCA AGGGCACGTC CCGCCTGGGC 
GACCTCTACG AGGAGGAGAT GCGGGAGCTG CGGCGGCAGG TGGACCAGCT CACCAACGAT 
AAGGCACGCG TGGAAGTGGA GCGGGACAAC CTCGCCGACG ACATCATGCG GCTGAGGGAG 
AAGTTGCAAG AAGAGATGCT TCAGAGGGAG GAGGCCGAGA ACACCCTGCA GTCCTTCAGA 
CAGGATGTTG ACAATGCCTC TCTGGCACGT CTTGATCTTG AGCGCAAAGT TGAGTCCCTG 
CAAGAGGAGA TTGTCTTCTT GAAGAAGCTT CATGATGAGG AAATCCGAGA ATTGCAGGCC 
CAACTCCAGG AACAGCACAT CCAAATCGAT ATGGATGTTT CTAAGCCTGA TCTCACTGCT 
GCCCTGCGTG ATGTTCGCCA ACAGTATGAA AGCGTTGCTG CTAAGAATCT TCAGGAAGCT 
GAAGAGTGGT ACAAGTCCAA ATTTGCAGAT CTCTCTGAAG CTGCTAATAG GAACAACGAT 
GCCCTGCGCC AGGCCAAACA AGAAGCCAAT GAGTACCGCA GACAGATCCA GTCTCTCACC 
TGCGAAGTTG ATGCTCTTAA GGGAAGCAAT GAGTCCCTGG AGCGCCAAAT GCGTGAAATG 
GAGGAGAATT TTGCTATGGA AGCTGCTAAC TACCAGGACA CTATTGGCCG TCTGCAGGAT 
GAGATTCAGA ACATGAAGGA AGAAATGGCT CGCCATCTGC GTGAGTACCA GGACCTGCTG 
AATGTAAAGA TGGCTCTTGA TATTGAGATT GCCACCTACA GAAAGCTGCT GGAGGGTGAA 
GAGAGCAGGA TTAACATGCC CATTCCAACC TTTGCTTCTT TGAACCTGAG AGAAACCAAC 
ATTGAGTCTC AGCCAATGGT TGACACTCAC TCAAAGAGGA CACTTCTAAT TAAGACCGTC 
GAAACTAGAG ATGGACAGGT TATTAATGAA ACTTCCCAGC ATCATGATGA CTTGGAGTAG