LOC100874919 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100874919
Entrez Gene ID	100874919
Full Name	serine/threonine-protein kinase BRSK2
Synonyms	Brsk2
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012298829.1	XP_012154219.1	serine/threonine-protein kinase BRSK2

Pathways from BioSystems
Gene Ontology

The following LOC100874919 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100874919 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe98889	XM_012298829.1 Latest version!	Megachile rotundata serine/threonine-protein kinase BRSK2 (LOC100874919), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100874919 ( XM_012298829.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012154219.1 Megachile rotundata serine/threonine-protein kinase BRSK2 (LOC100874919), mRNA.

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CloneID	OMe98889
Clone ID Related Accession (Same CDS sequence)	XM_012298829.1
Accession Version	XM_012298829.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	serine/threonine-protein kinase BRSK2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797096.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGGCA AGGAGAGCCC CGTCGGGTCC AACACCCAGG AGACCCATCA GTACGTTGGC 
CCTTATCGAC TGGAGAAAAC TTTGGGGAAA GGGCAAACCG GATTGGTGAA GCTCGGGGTC 
CACTGCGTGT TGGGCAAGAA AGTGGCTATC AAGATCATCA ACCGGGAAAA ATTGTCGGAG 
TCCGTGTTGA TGAAGGTAGA ACGAGAGATA GCTATCATGA AACTGATCGA TCATCCTCAC 
GTGCTCGGCC TGTCGGATGT ATACGAGAAC AAGAAATACC TATATCTCGT TCTGGAACAC 
GTTTCCGGCG GGGAGCTTTT CGACTATTTA GTGAAAAAAG GTAGACTAAC GCCGAAGGAA 
GCGAGGAGAT TTTTTCGGCA GATCATTTCT GCGTTAGATT TTTGTCATAG CCATAGTATA 
TGTCACAGAG ACTTGAAGCC TGAAAATCTG TTGCTGGACG AGAAGAATAA TATTAAGATC 
GCGGATTTCG GGATGGCGTC GTTGCAACCT GCTGGATCAA TGTTAGAAAC TAGCTGCGGT 
TCCCCTCATT ACGCCTGTCC CGAAGTCATT CGGGGTGAAA AGTACGACGG GAGGAAAGCG 
GACGTTTGGT CGTGCGGGGT GATACTTTAC GCGCTTCTGG TAGGCGCGTT GCCCTTCGAC 
GACGACAATT TAAGAAACTT GTTGGAAAAA GTGAAGCGCG GTATGTTTTA CATACCGCAT 
TTCGTGCCAC CCGAGTGCCA GAATCTTCTG AAGGGAATGA TCGAGGTCGA TCCGGACAAG 
AGGCTAACGC TGGCGGAAAT AAATAGGCAC GTGTGGGTGA CGGCGGCGGG CAAGGGCGAA 
TTGGAACTGG AACTGTCGAT GATGGACGTG GTACAGACTC ACGTTATTCC GTCCGTGGAA 
GCAATCGATC CAGACGTGTT GCAGGCGATC GCGAGCCTCG GCTGCTTCAA GGAACGGGAC 
AAACTTATCC AAGAACTCCT CAGCCCGAAC CACAATACAG AGAAGGTGAT ATATTTCCTG 
TTGCTCGAGA GGAAACGCAG AAGACCGGCG TGCGAGGATG AACTAGAAGT AATGAGAGGA 
GGAATGCGAG GTTCAGCGGG ACAATTGGAG GCGGATCCAC CGAGGAAACG AGTAGACACG 
TGTCGAGTGA ACGGAAGTAC AGCGCTGAAT CTCGGAGAAA TCAGCCATGG ATCTCCTTTG 
ACTCCCAGAA GGCAGTCCAG CACCAAGCAT CACGCTCGTC GTTCGCCGAG TACGGGTTGC 
CACACACACG CGTCGCATTC GCATGCCTCG ACGCCAGGCA GCTCGCCGTT GCACGGCTCG 
AACGCCGTCG CGGGTCTGCT CAGTCCCCAC AGAGCACCTC CGTCGTCGCA TTTAGGACAG 
ACGGCGAGTC CTCATCGACT GTCCACGGTT TCGAGCACCG CGGGCAACGG AAACACCGCC 
AATCCACCCG CCGCAGTTCC AATCCCGGCA TTACCCAGCG TGGGAATAGA GATAAACGAC 
GTTCAACAGG TAGTCGCGGT CGGCGTTACA CCGCCAGGAT CGCCTCACAC CGGAGCCGGA 
TCCGGCGCCC ATCACTGGAG ATCCAGACTA ACGACCATCA AGAACTCCTT CCTTGGCAGT 
CCTCGATTTC ATCGGCGTAA ATTGCTCACG AGTACCGAGG AGGTACACCT CACTCCGGAT 
TCTTCCCCGG AACTTACAAA GAAATCGTGG TTCGGCAGCT TGATGACCAC GGAAAAGGAC 
GAAACGTTTA CCGTTCTCGT TAAAGGGAAA GCATTAGCTA GCGTCAAAGC AGATTTGATT 
CACGCTTTTT TATCGATAGC GGAATTGTCT CACAGCGTGA GCTCGCCCAT GTCCTTCAGA 
GTGGAATACA AAAGAGGAAG CACAGCACCG GCAATGTTTC AAAGACAAGT GCGCTTTCAA 
GTGGACATTA GCGCTATTTC GAAGCAGCCG AACGAACCAC TGTTCGCCAT CACTTTCACT 
CTATTGAGCG GAAACATTCG ACGATTCCGA AGAGTCTGCG AACACATTCA ATCGCAGGTC 
TGCTCGAGAA ACGTAGGAAT CAGTTTGGGA GGACAAAGCA GAGCAGCACC ACCCAGTCCG 
CGAGCATCGC GAAAATTTGC CACGGAGATG AGCGAAAGTT CCAGTTGCGG TAGCGACACT 
AGCGAACGAC TGTTTCTCAG TCCGCACCCA GCTACCAGAC AGGTAGTCTG TTTCAACAAG 
ATCGATTCGG ACATCGAATC GGACACGTCC GTGTTCGACG TGAAATCACC AAGCAGGGAA 
AACGGTCGTC GAAGTTCGAC GTCGACGAAC AACAACAATC CATTGCCAGG AGACGCGACG 
CCGACGCCCG GAAGTCCGAC GAAGGCGGTT CGCAGCAACT CGGAGAGTCA GAACACGCCG 
GAAAAGAAAT GCGTAACCAC GATGAGCGGC AACAATATAG CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012154219.1
CDS	261..2705
Translation	MQGKESPVGSNTQETHQYVGPYRLEKTLGKGQTGLVKLGVHCVLGKKVAIKIINREKLSESVLMKVEREIAIMKLIDHPHVLGLSDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARRFFRQIISALDFCHSHSICHRDLKPENLLLDEKNNIKIADFGMASLQPAGSMLETSCGSPHYACPEVIRGEKYDGRKADVWSCGVILYALLVGALPFDDDNLRNLLEKVKRGMFYIPHFVPPECQNLLKGMIEVDPDKRLTLAEINRHVWVTAAGKGELELELSMMDVVQTHVIPSVEAIDPDVLQAIASLGCFKERDKLIQELLSPNHNTEKVIYFLLLERKRRRPACEDELEVMRGGMRGSAGQLEADPPRKRVDTCRVNGSTALNLGEISHGSPLTPRRQSSTKHHARRSPSTGCHTHASHSHASTPGSSPLHGSNAVAGLLSPHRAPPSSHLGQTASPHRLSTVSSTAGNGNTANPPAAVPIPALPSVGIEINDVQQVVAVGVTPPGSPHTGAGSGAHHWRSRLTTIKNSFLGSPRFHRRKLLTSTEEVHLTPDSSPELTKKSWFGSLMTTEKDETFTVLVKGKALASVKADLIHAFLSIAELSHSVSSPMSFRVEYKRGSTAPAMFQRQVRFQVDISAISKQPNEPLFAITFTLLSGNIRRFRRVCEHIQSQVCSRNVGISLGGQSRAAPPSPRASRKFATEMSESSSCGSDTSERLFLSPHPATRQVVCFNKIDSDIESDTSVFDVKSPSRENGRRSSTSTNNNNPLPGDATPTPGSPTKAVRSNSESQNTPEKKCVTTMSGNNIA

Target ORF information:

RefSeq Version	XM_012298829.1
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata serine/threonine-protein kinase BRSK2 (LOC100874919), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012298829.1

ORF Insert Sequence:

ATGCAGGGCA AGGAGAGCCC CGTCGGGTCC AACACCCAGG AGACCCATCA GTACGTTGGC 
CCTTATCGAC TGGAGAAAAC TTTGGGGAAA GGGCAAACCG GATTGGTGAA GCTCGGGGTC 
CACTGCGTGT TGGGCAAGAA AGTGGCTATC AAGATCATCA ACCGGGAAAA ATTGTCGGAG 
TCCGTGTTGA TGAAGGTAGA ACGAGAGATA GCTATCATGA AACTGATCGA TCATCCTCAC 
GTGCTCGGCC TGTCGGATGT ATACGAGAAC AAGAAATACC TATATCTCGT TCTGGAACAC 
GTTTCCGGCG GGGAGCTTTT CGACTATTTA GTGAAAAAAG GTAGACTAAC GCCGAAGGAA 
GCGAGGAGAT TTTTTCGGCA GATCATTTCT GCGTTAGATT TTTGTCATAG CCATAGTATA 
TGTCACAGAG ACTTGAAGCC TGAAAATCTG TTGCTGGACG AGAAGAATAA TATTAAGATC 
GCGGATTTCG GGATGGCGTC GTTGCAACCT GCTGGATCAA TGTTAGAAAC TAGCTGCGGT 
TCCCCTCATT ACGCCTGTCC CGAAGTCATT CGGGGTGAAA AGTACGACGG GAGGAAAGCG 
GACGTTTGGT CGTGCGGGGT GATACTTTAC GCGCTTCTGG TAGGCGCGTT GCCCTTCGAC 
GACGACAATT TAAGAAACTT GTTGGAAAAA GTGAAGCGCG GTATGTTTTA CATACCGCAT 
TTCGTGCCAC CCGAGTGCCA GAATCTTCTG AAGGGAATGA TCGAGGTCGA TCCGGACAAG 
AGGCTAACGC TGGCGGAAAT AAATAGGCAC GTGTGGGTGA CGGCGGCGGG CAAGGGCGAA 
TTGGAACTGG AACTGTCGAT GATGGACGTG GTACAGACTC ACGTTATTCC GTCCGTGGAA 
GCAATCGATC CAGACGTGTT GCAGGCGATC GCGAGCCTCG GCTGCTTCAA GGAACGGGAC 
AAACTTATCC AAGAACTCCT CAGCCCGAAC CACAATACAG AGAAGGTGAT ATATTTCCTG 
TTGCTCGAGA GGAAACGCAG AAGACCGGCG TGCGAGGATG AACTAGAAGT AATGAGAGGA 
GGAATGCGAG GTTCAGCGGG ACAATTGGAG GCGGATCCAC CGAGGAAACG AGTAGACACG 
TGTCGAGTGA ACGGAAGTAC AGCGCTGAAT CTCGGAGAAA TCAGCCATGG ATCTCCTTTG 
ACTCCCAGAA GGCAGTCCAG CACCAAGCAT CACGCTCGTC GTTCGCCGAG TACGGGTTGC 
CACACACACG CGTCGCATTC GCATGCCTCG ACGCCAGGCA GCTCGCCGTT GCACGGCTCG 
AACGCCGTCG CGGGTCTGCT CAGTCCCCAC AGAGCACCTC CGTCGTCGCA TTTAGGACAG 
ACGGCGAGTC CTCATCGACT GTCCACGGTT TCGAGCACCG CGGGCAACGG AAACACCGCC 
AATCCACCCG CCGCAGTTCC AATCCCGGCA TTACCCAGCG TGGGAATAGA GATAAACGAC 
GTTCAACAGG TAGTCGCGGT CGGCGTTACA CCGCCAGGAT CGCCTCACAC CGGAGCCGGA 
TCCGGCGCCC ATCACTGGAG ATCCAGACTA ACGACCATCA AGAACTCCTT CCTTGGCAGT 
CCTCGATTTC ATCGGCGTAA ATTGCTCACG AGTACCGAGG AGGTACACCT CACTCCGGAT 
TCTTCCCCGG AACTTACAAA GAAATCGTGG TTCGGCAGCT TGATGACCAC GGAAAAGGAC 
GAAACGTTTA CCGTTCTCGT TAAAGGGAAA GCATTAGCTA GCGTCAAAGC AGATTTGATT 
CACGCTTTTT TATCGATAGC GGAATTGTCT CACAGCGTGA GCTCGCCCAT GTCCTTCAGA 
GTGGAATACA AAAGAGGAAG CACAGCACCG GCAATGTTTC AAAGACAAGT GCGCTTTCAA 
GTGGACATTA GCGCTATTTC GAAGCAGCCG AACGAACCAC TGTTCGCCAT CACTTTCACT 
CTATTGAGCG GAAACATTCG ACGATTCCGA AGAGTCTGCG AACACATTCA ATCGCAGGTC 
TGCTCGAGAA ACGTAGGAAT CAGTTTGGGA GGACAAAGCA GAGCAGCACC ACCCAGTCCG 
CGAGCATCGC GAAAATTTGC CACGGAGATG AGCGAAAGTT CCAGTTGCGG TAGCGACACT 
AGCGAACGAC TGTTTCTCAG TCCGCACCCA GCTACCAGAC AGGTAGTCTG TTTCAACAAG 
ATCGATTCGG ACATCGAATC GGACACGTCC GTGTTCGACG TGAAATCACC AAGCAGGGAA 
AACGGTCGTC GAAGTTCGAC GTCGACGAAC AACAACAATC CATTGCCAGG AGACGCGACG 
CCGACGCCCG GAAGTCCGAC GAAGGCGGTT CGCAGCAACT CGGAGAGTCA GAACACGCCG 
GAAAAGAAAT GCGTAACCAC GATGAGCGGC AACAATATAG CGTGA