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KLRK1 cDNA ORF clone, Mus musculus(House mouse)

Gene Symbol KLRK1
Entrez Gene ID 27007
Full Name killer cell lectin-like receptor subfamily K, member 1
Synonyms D6H12S2489E,NKG2-D,Nkg2d
General protein information
Preferred Names

killer cell lectin-like receptor subfamily K, member 1

Names

NKG2-D type II integral membrane protein
NK cell receptor D
NKG2-D-activating NK receptor
natural killer cell group 2D

Gene Type protein-coding
Organism Mus musculus(House mouse)
Genome

6

6 F3|6 63.44 cM


mRNA and Protein(s)

mRNA Protein Name
NM_001286018.1 NP_001272947.1 NKG2-D type II integral membrane protein isoform c
NM_001083322.2 NP_001076791.1 NKG2-D type II integral membrane protein isoform b
XM_011241369.2 XP_011239671.1 NKG2-D type II integral membrane protein isoform X1
NM_033078.4 NP_149069.1 NKG2-D type II integral membrane protein isoform a



Rattus norvegicus (Norway rat) Klrk1 NP_598196.1
Bos taurus (cattle) KLRK1 NP_001068607.1
Macaca mulatta (rhesus monkey) NKG2D NP_001028061.1
Mus musculus (house mouse) Klrk1 NP_149069.1
Pan troglodytes (chimpanzee) KLRK1 NP_001009059.1
Canis lupus familiaris (dog) KLRK1 XP_005637217.1


Related articles in PubMed

Ezh2 regulates differentiation and function of natural killer cells through histone methyltransferase activity.
Yin J, Leavenworth JW, Li Y, Luo Q, Xie H, Liu X, Huang S, Yan H, Fu Z, Zhang LY, Zhang L, Hao J, Wu X, Deng X, Roberts CW, Orkin SH, Cantor H, Wang X
Proceedings of the National Academy of Sciences of the United States of America112(52)15988-93(2015 Dec)

Critical role of the NKG2D receptor for NK cell-mediated control and immune escape of B-cell lymphoma.
Belting L, Hömberg N, Przewoznik M, Brenner C, Riedel T, Flatley A, Polić B, Busch DH, Röcken M, Mocikat R
European journal of immunology45(9)2593-601(2015 Sep)

NKG2D expression by CD8+ T cells contributes to GVHD and GVT effects in a murine model of allogeneic HSCT.
Karimi MA, Bryson JL, Richman LP, Fesnak AD, Leichner TM, Satake A, Vonderheide RH, Raulet DH, Reshef R, Kambayashi T
Blood125(23)3655-63(2015 Jun)

Antitumor immunity. A shed NKG2D ligand that promotes natural killer cell activation and tumor rejection.
Deng W, Gowen BG, Zhang L, Wang L, Lau S, Iannello A, Xu J, Rovis TL, Xiong N, Raulet DH
Science (New York, N.Y.)348(6230)136-9(2015 Apr)

A Peptide Mimetic of 5-Acetylneuraminic Acid-Galactose Binds with High Avidity to Siglecs and NKG2D.
Eggink LL, Spyroulias GA, Jones NG, Hanson CV, Hoober JK
PloS one10(6)e0130532(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study demonstrates that the effect of NKG2D on B1a cell development occurs at a developmental stage that precedes the common lymphoid progenitor; findings reveal an unexpected new role for NKG2D in the regulation of B1a cell development
Title: NKG2D Promotes B1a Cell Development and Protection against Bacterial Infection.

Sirt6 binds to promoters of NKG2D ligand genes and regulates the H3K9 and H3K56 acetylation levels
Title: Epigenetic regulation of NKG2D ligands is involved in exacerbated atherosclerosis development in Sirt6 heterozygous mice.

The results show that NK cells and the NKG2D receptor play a role in control of lymphomas, and that selection of NKG2D-ligand (MULT1) loss mutants provides a mechanism for tumor escape.
Title: Critical role of the NKG2D receptor for NK cell-mediated control and immune escape of B-cell lymphoma.

these data support a novel role for NKG2D expression by CD8(+) T cells during allogeneic HSCT, which could be potentially therapeutically exploited to separate GVHD from GVT effects.
Title: NKG2D expression by CD8+ T cells contributes to GVHD and GVT effects in a murine model of allogeneic HSCT.

These results support the conclusion that svH1C mimics Neu5Ac-containing sequences and interacts with cell-surface receptor NKG2D, which contains a lectin-like domain
Title: A Peptide Mimetic of 5-Acetylneuraminic Acid-Galactose Binds with High Avidity to Siglecs and NKG2D.

The following KLRK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KLRK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OMu19902 NM_033078.4
Latest version!
Mus musculus killer cell lectin-like receptor subfamily K, member 1 (Klrk1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$134.50-$188.30
$269.00
OMu23105 NM_001083322.2
Latest version!
Mus musculus killer cell lectin-like receptor subfamily K, member 1 (Klrk1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$134.50-$188.30
$269.00
OMu23094 NM_001286018.1
Latest version!
Mus musculus killer cell lectin-like receptor subfamily K, member 1 (Klrk1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OMu69255 XM_011241369.2
Latest version!
Mus musculus killer cell lectin-like receptor subfamily K, member 1 (Klrk1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OMu19902
Clone ID Related Accession (Same CDS sequence) NM_033078.4
Accession Version NM_033078.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 699bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-09-10
Organism Mus musculus(House mouse)
Product NKG2-D type II integral membrane protein isoform a
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AA174479.1, AK040436.1 and AC171002.2. On Oct 26, 2013 this sequence version replaced NM_033078.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by mRNA and EST alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK040436.1, AF039026.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCATTGA TTCGTGATCG AAAGTCTCAT CACTCAGAGA TGAGCAAATG CCATAATTAC 
GACCTCAAGC CAGCAAAGTG GGATACTTCT CAAGAACAAC AGAAACAAAG ATTAGCACTA
ACTACCAGTC AACCTGGAGA AAATGGTATC ATAAGAGGAA GATACCCTAT AGAAAAACTC
AAAATATCTC CAATGTTCGT TGTTCGAGTC CTTGCTATAG CCTTGGCAAT TCGATTCACC
CTTAACACAT TGATGTGGCT TGCCATTTTC AAAGAGACGT TTCAGCCAGT ATTGTGCAAC
AAGGAAGTCC CAGTTTCCTC AAGAGAGGGC TACTGTGGCC CATGCCCTAA CAACTGGATA
TGTCACAGAA ACAACTGTTA CCAATTTTTT AATGAAGAGA AAACCTGGAA CCAGAGCCAA
GCTTCCTGTT TGTCTCAAAA TTCCAGCCTT CTGAAGATAT ACAGTAAAGA AGAACAGGAT
TTCTTAAAGC TGGTTAAGTC CTATCACTGG ATGGGACTGG TCCAGATCCC AGCAAATGGC
TCCTGGCAGT GGGAAGATGG CTCCTCTCTC TCATACAATC AGTTAACTCT GGTGGAAATA
CCAAAAGGAT CCTGTGCTGT CTATGGCTCA AGCTTTAAGG CTTACACAGA AGACTGTGCA
AATCTAAACA CGTACATCTG CATGAAAAGG GCGGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_149069.1
CDS111..809
Misc Feature(1)87..89
Misc Feature(2)309..377
Misc Feature(3)453..797
Misc Feature(4)order(612..614,693..695,747..749,753..758)
Exon (1)1..147
Exon (2)148..255
Exon (3)256..294
Exon (4)295..399
Exon (5)400..435
Exon (6)436..587
Exon (7)588..691
Exon (8)692..3272
Translation

Target ORF information:

RefSeq Version NM_033078.4
Organism Mus musculus(House mouse)
Definition Mus musculus killer cell lectin-like receptor subfamily K, member 1 (Klrk1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_033078.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCATTGA TTCGTGATCG AAAGTCTCAT CACTCAGAGA TGAGCAAATG CCATAATTAC 
GACCTCAAGC CAGCAAAGTG GGATACTTCT CAAGAACAAC AGAAACAAAG ATTAGCACTA
ACTACCAGTC AACCTGGAGA AAATGGTATC ATAAGAGGAA GATACCCTAT AGAAAAACTC
AAAATATCTC CAATGTTCGT TGTTCGAGTC CTTGCTATAG CCTTGGCAAT TCGATTCACC
CTTAACACAT TGATGTGGCT TGCCATTTTC AAAGAGACGT TTCAGCCAGT ATTGTGCAAC
AAGGAAGTCC CAGTTTCCTC AAGAGAGGGC TACTGTGGCC CATGCCCTAA CAACTGGATA
TGTCACAGAA ACAACTGTTA CCAATTTTTT AATGAAGAGA AAACCTGGAA CCAGAGCCAA
GCTTCCTGTT TGTCTCAAAA TTCCAGCCTT CTGAAGATAT ACAGTAAAGA AGAACAGGAT
TTCTTAAAGC TGGTTAAGTC CTATCACTGG ATGGGACTGG TCCAGATCCC AGCAAATGGC
TCCTGGCAGT GGGAAGATGG CTCCTCTCTC TCATACAATC AGTTAACTCT GGTGGAAATA
CCAAAAGGAT CCTGTGCTGT CTATGGCTCA AGCTTTAAGG CTTACACAGA AGACTGTGCA
AATCTAAACA CGTACATCTG CATGAAAAGG GCGGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu23105
Clone ID Related Accession (Same CDS sequence) NM_001083322.2
Accession Version NM_001083322.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 660bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-09-10
Organism Mus musculus(House mouse)
Product NKG2-D type II integral membrane protein isoform b
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC171002.2, BY207066.1 and AF030313.1. On Oct 26, 2013 this sequence version replaced NM_001083322.1. Transcript Variant: This variant (2) differs in the 5' UTR and the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform b, which has a shorter and distinct N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by mRNA and EST alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF030313.1, AA178606.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGAGCAAAT GCCATAATTA CGACCTCAAG CCAGCAAAGT GGGATACTTC TCAAGAACAA 
CAGAAACAAA GATTAGCACT AACTACCAGT CAACCTGGAG AAAATGGTAT CATAAGAGGA
AGATACCCTA TAGAAAAACT CAAAATATCT CCAATGTTCG TTGTTCGAGT CCTTGCTATA
GCCTTGGCAA TTCGATTCAC CCTTAACACA TTGATGTGGC TTGCCATTTT CAAAGAGACG
TTTCAGCCAG TATTGTGCAA CAAGGAAGTC CCAGTTTCCT CAAGAGAGGG CTACTGTGGC
CCATGCCCTA ACAACTGGAT ATGTCACAGA AACAACTGTT ACCAATTTTT TAATGAAGAG
AAAACCTGGA ACCAGAGCCA AGCTTCCTGT TTGTCTCAAA ATTCCAGCCT TCTGAAGATA
TACAGTAAAG AAGAACAGGA TTTCTTAAAG CTGGTTAAGT CCTATCACTG GATGGGACTG
GTCCAGATCC CAGCAAATGG CTCCTGGCAG TGGGAAGATG GCTCCTCTCT CTCATACAAT
CAGTTAACTC TGGTGGAAAT ACCAAAAGGA TCCTGTGCTG TCTATGGCTC AAGCTTTAAG
GCTTACACAG AAGACTGTGC AAATCTAAAC ACGTACATCT GCATGAAAAG GGCGGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001076791.1
CDS57..716
Misc Feature(1)18..20
Misc Feature(2)360..704
Misc Feature(3)order(519..521,600..602,654..656,660..665)
Exon (1)1..54
Exon (2)55..162
Exon (3)163..201
Exon (4)202..306
Exon (5)307..342
Exon (6)343..494
Exon (7)495..598
Exon (8)599..3179
Translation

Target ORF information:

RefSeq Version NM_001083322.2
Organism Mus musculus(House mouse)
Definition Mus musculus killer cell lectin-like receptor subfamily K, member 1 (Klrk1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001083322.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGAGCAAAT GCCATAATTA CGACCTCAAG CCAGCAAAGT GGGATACTTC TCAAGAACAA 
CAGAAACAAA GATTAGCACT AACTACCAGT CAACCTGGAG AAAATGGTAT CATAAGAGGA
AGATACCCTA TAGAAAAACT CAAAATATCT CCAATGTTCG TTGTTCGAGT CCTTGCTATA
GCCTTGGCAA TTCGATTCAC CCTTAACACA TTGATGTGGC TTGCCATTTT CAAAGAGACG
TTTCAGCCAG TATTGTGCAA CAAGGAAGTC CCAGTTTCCT CAAGAGAGGG CTACTGTGGC
CCATGCCCTA ACAACTGGAT ATGTCACAGA AACAACTGTT ACCAATTTTT TAATGAAGAG
AAAACCTGGA ACCAGAGCCA AGCTTCCTGT TTGTCTCAAA ATTCCAGCCT TCTGAAGATA
TACAGTAAAG AAGAACAGGA TTTCTTAAAG CTGGTTAAGT CCTATCACTG GATGGGACTG
GTCCAGATCC CAGCAAATGG CTCCTGGCAG TGGGAAGATG GCTCCTCTCT CTCATACAAT
CAGTTAACTC TGGTGGAAAT ACCAAAAGGA TCCTGTGCTG TCTATGGCTC AAGCTTTAAG
GCTTACACAG AAGACTGTGC AAATCTAAAC ACGTACATCT GCATGAAAAG GGCGGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu23094
Clone ID Related Accession (Same CDS sequence) NM_001286018.1
Accession Version NM_001286018.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 678bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-09-10
Organism Mus musculus(House mouse)
Product NKG2-D type II integral membrane protein isoform c
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC171002.2. Transcript Variant: This variant (3) differs in the 5' UTR and the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform c, which contains a distinct N-terminus and an additional internal in-frame segment, and is shorter, compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK170499.1, BY748420.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
ATGAGCAAAT GCCATAATTA CGACCTCAAG CCAGCAAAGT GGGATACTTC TCAAGAACAA 
CAGAAACAAA GATTAGCACT AACTACCAGT CAACCTGGAG AAAATGGTAT CATAAGAGGA
AGATACCCTA TAGAAAAACT CAAAATATCT CCAATGTTCG TTGTTCGAGT CCTTGCTATA
GCCTTGGCAA TTCGATTCAC CCTTAACACA TTGATGTGGC TTGCCATTTT CAAAGAGACG
TTTCAGCCAG TTATAATTAT CTTGACAGTA TTGTGCAACA AGGAAGTCCC AGTTTCCTCA
AGAGAGGGCT ACTGTGGCCC ATGCCCTAAC AACTGGATAT GTCACAGAAA CAACTGTTAC
CAATTTTTTA ATGAAGAGAA AACCTGGAAC CAGAGCCAAG CTTCCTGTTT GTCTCAAAAT
TCCAGCCTTC TGAAGATATA CAGTAAAGAA GAACAGGATT TCTTAAAGCT GGTTAAGTCC
TATCACTGGA TGGGACTGGT CCAGATCCCA GCAAATGGCT CCTGGCAGTG GGAAGATGGC
TCCTCTCTCT CATACAATCA GTTAACTCTG GTGGAAATAC CAAAAGGATC CTGTGCTGTC
TATGGCTCAA GCTTTAAGGC TTACACAGAA GACTGTGCAA ATCTAAACAC GTACATCTGC
ATGAAAAGGG CGGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001272947.1
CDS57..734
Misc Feature(1)18..20
Misc Feature(2)264..725
Misc Feature(3)378..722
Misc Feature(4)order(537..539,618..620,672..674,678..683)
Exon (1)1..54
Exon (2)55..162
Exon (3)163..201
Exon (4)202..306
Exon (5)307..360
Exon (6)361..512
Exon (7)513..616
Exon (8)617..3197
Translation

Target ORF information:

RefSeq Version NM_001286018.1
Organism Mus musculus(House mouse)
Definition Mus musculus killer cell lectin-like receptor subfamily K, member 1 (Klrk1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001286018.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGAGCAAAT GCCATAATTA CGACCTCAAG CCAGCAAAGT GGGATACTTC TCAAGAACAA 
CAGAAACAAA GATTAGCACT AACTACCAGT CAACCTGGAG AAAATGGTAT CATAAGAGGA
AGATACCCTA TAGAAAAACT CAAAATATCT CCAATGTTCG TTGTTCGAGT CCTTGCTATA
GCCTTGGCAA TTCGATTCAC CCTTAACACA TTGATGTGGC TTGCCATTTT CAAAGAGACG
TTTCAGCCAG TTATAATTAT CTTGACAGTA TTGTGCAACA AGGAAGTCCC AGTTTCCTCA
AGAGAGGGCT ACTGTGGCCC ATGCCCTAAC AACTGGATAT GTCACAGAAA CAACTGTTAC
CAATTTTTTA ATGAAGAGAA AACCTGGAAC CAGAGCCAAG CTTCCTGTTT GTCTCAAAAT
TCCAGCCTTC TGAAGATATA CAGTAAAGAA GAACAGGATT TCTTAAAGCT GGTTAAGTCC
TATCACTGGA TGGGACTGGT CCAGATCCCA GCAAATGGCT CCTGGCAGTG GGAAGATGGC
TCCTCTCTCT CATACAATCA GTTAACTCTG GTGGAAATAC CAAAAGGATC CTGTGCTGTC
TATGGCTCAA GCTTTAAGGC TTACACAGAA GACTGTGCAA ATCTAAACAC GTACATCTGC
ATGAAAAGGG CGGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu69255
Clone ID Related Accession (Same CDS sequence) XM_011241369.2
Accession Version XM_011241369.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 585bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-22
Organism Mus musculus(House mouse)
Product NKG2-D type II integral membrane protein isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011241369.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
ATGGCATTGA TTCGTGATCG AAAGTCTCAT CACTCAGAGA TGAGCAAATG CCATAATTAC 
GACCTCAAGC CAGCAAAGTG GGATACTTCT CAAGAACAAC AGAAACAAAG ATTAGCACTA
ACTACCAGTC AACCTGGAGA AAATGGTATC ATAAGAGGAA GATACCCTAT AGAAAAACTC
AAAATATCTC CAATGTTCGT TGTTCGAGTC CTTGCTATAG CCTTGGCAAT TCGATTCACC
CTTAACACAT TGATGTGGCT TGCCATTTTC AAAGAGACGT TTCAGCCAGT ATTGTGCAAC
AAGGAAGTCC CAGTTTCCTC AAGAGAGGGC TACTGTGGCC CATGCCCTAA CAACTGGATA
TGTCACAGAA ACAACTGTTA CCAATTTTTT AATGAAGAGA AAACCTGGAA CCAGAGCCAA
GCTTCCTGTT TGTCTCAAAA TTCCAGCCTT CTGAAGATAT ACAGTAAAGA AGAACAGGAT
TTCTTAAAGC TGGTTAAGTC CTATCACTGG ATGGGACTGG TCCAGATCCC AGCAAATGGC
TCCTGGCAGT GGGAAGATGG CTCCTCTCTC TCATACAATC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011239671.1
CDS166..750
Misc Feature(1)508..>744
Translation

Target ORF information:

RefSeq Version XM_011241369.2
Organism Mus musculus(House mouse)
Definition Mus musculus killer cell lectin-like receptor subfamily K, member 1 (Klrk1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011241369.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCATTGA TTCGTGATCG AAAGTCTCAT CACTCAGAGA TGAGCAAATG CCATAATTAC 
GACCTCAAGC CAGCAAAGTG GGATACTTCT CAAGAACAAC AGAAACAAAG ATTAGCACTA
ACTACCAGTC AACCTGGAGA AAATGGTATC ATAAGAGGAA GATACCCTAT AGAAAAACTC
AAAATATCTC CAATGTTCGT TGTTCGAGTC CTTGCTATAG CCTTGGCAAT TCGATTCACC
CTTAACACAT TGATGTGGCT TGCCATTTTC AAAGAGACGT TTCAGCCAGT ATTGTGCAAC
AAGGAAGTCC CAGTTTCCTC AAGAGAGGGC TACTGTGGCC CATGCCCTAA CAACTGGATA
TGTCACAGAA ACAACTGTTA CCAATTTTTT AATGAAGAGA AAACCTGGAA CCAGAGCCAA
GCTTCCTGTT TGTCTCAAAA TTCCAGCCTT CTGAAGATAT ACAGTAAAGA AGAACAGGAT
TTCTTAAAGC TGGTTAAGTC CTATCACTGG ATGGGACTGG TCCAGATCCC AGCAAATGGC
TCCTGGCAGT GGGAAGATGG CTCCTCTCTC TCATACAATC AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Ezh2 regulates differentiation and function of natural killer cells through histone methyltransferase activity.
Proceedings of the National Academy of Sciences of the United States of America112(52)15988-93(2015 Dec)
Yin J,Leavenworth JW,Li Y,Luo Q,Xie H,Liu X,Huang S,Yan H,Fu Z,Zhang LY,Zhang L,Hao J,Wu X,Deng X,Roberts CW,Orkin SH,Cantor H,Wang X


book

Critical role of the NKG2D receptor for NK cell-mediated control and immune escape of B-cell lymphoma.
European journal of immunology45(9)2593-601(2015 Sep)
Belting L,Hömberg N,Przewoznik M,Brenner C,Riedel T,Flatley A,Polić B,Busch DH,Röcken M,Mocikat R


book

NKG2D expression by CD8+ T cells contributes to GVHD and GVT effects in a murine model of allogeneic HSCT.
Blood125(23)3655-63(2015 Jun)
Karimi MA,Bryson JL,Richman LP,Fesnak AD,Leichner TM,Satake A,Vonderheide RH,Raulet DH,Reshef R,Kambayashi T


book

Antitumor immunity. A shed NKG2D ligand that promotes natural killer cell activation and tumor rejection.
Science (New York, N.Y.)348(6230)136-9(2015 Apr)
Deng W,Gowen BG,Zhang L,Wang L,Lau S,Iannello A,Xu J,Rovis TL,Xiong N,Raulet DH


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A Peptide Mimetic of 5-Acetylneuraminic Acid-Galactose Binds with High Avidity to Siglecs and NKG2D.
PloS one10(6)e0130532(2015)
Eggink LL,Spyroulias GA,Jones NG,Hanson CV,Hoober JK


 
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