YBX1 cDNA ORF clone, Oryctolagus cuniculus(rabbit)

Gene
Clones
gRNAs

Summary

Gene Symbol	YBX1
Entrez Gene ID	100009583
Full Name	Y box binding protein 1
Synonyms	YB-1
Gene Type	protein-coding
Organism	Oryctolagus cuniculus(rabbit)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001082785.1	NP_001076254.1	Y-box-binding protein 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

YB-1 synthesis is regulated by mTOR signaling pathway.
Lyabin DN, Eliseeva IA, Ovchinnikov LP
PloS one7(12)e52527(2012)

Interplay between Y-box-binding protein 1 (YB-1) and poly(A) binding protein (PABP) in specific regulation of YB-1 mRNA translation.
Lyabin DN, Eliseeva IA, Skabkina OV, Ovchinnikov LP
RNA biology8(5)883-92(2011 Sep-Oct)

Akt-mediated YB-1 phosphorylation activates translation of silent mRNA species.
Evdokimova V, Ruzanov P, Anglesio MS, Sorokin AV, Ovchinnikov LP, Buckley J, Triche TJ, Sonenberg N, Sorensen PH
Molecular and cellular biology26(1)277-92(2006 Jan)

Proteasome-mediated cleavage of the Y-box-binding protein 1 is linked to DNA-damage stress response.
Sorokin AV, Selyutina AA, Skabkin MA, Guryanov SG, Nazimov IV, Richard C, Th'ng J, Yau J, Sorensen PH, Ovchinnikov LP, Evdokimova V
The EMBO journal24(20)3602-12(2005 Oct)

The mRNA-binding protein YB-1 (p50) prevents association of the eukaryotic initiation factor eIF4G with mRNA and inhibits protein synthesis at the initiation stage.
Nekrasov MP, Ivshina MP, Chernov KG, Kovrigina EA, Evdokimova VM, Thomas AA, Hershey JW, Ovchinnikov LP
The Journal of biological chemistry278(16)13936-43(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The amounts of YB-1 and YB-1 mRNA in rabbit organs and several cell lines.
Title: YB-1 synthesis is regulated by mTOR signaling pathway.

results prove direct inhibitory action of YB-1 on its mRNA translation, while the positive effect of PABP is realized through displacing YB-1 from the regulatory element
Title: Interplay between Y-box-binding protein 1 (YB-1) and poly(A) binding protein (PABP) in specific regulation of YB-1 mRNA translation.

The following YBX1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the YBX1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOb30233	NM_001082785.1 Latest version!	Oryctolagus cuniculus Y box binding protein 1 (YBX1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

YBX1 ( NM_001082785.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> NP_001076254.1 Oryctolagus cuniculus Y box binding protein 1 (YBX1), mRNA.

Price & Availability↑

CloneID	OOb30233
Clone ID Related Accession (Same CDS sequence)	NM_001082785.1
Accession Version	NM_001082785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Oryctolagus cuniculus(rabbit)
Product	Y-box-binding protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U16821.1. Sequence Note: This sequence has been modified as follows: removed 19 bp suspected to be vector contamination from the 5' end. ##Evidence-Data-START## Transcript exon combination :: U16821.1 [ECO:0000332] ##Evidence-Data-END##

ATGAGCAGCG AGGCCGAGAC CCAGCAGCCG CCCGCCGCCC CCCCCGCCGC CCCCGCCCTC 
AGCGCCGCCG AAACCAAGCC CGGCACCACG GGCAGCGGCG CAGGGAGCGG TGGCCCGGGC 
GGCCTCACAT CGGCGGCGCC TGCCGGCGGG GACAAGAAGG TCATCGCAAC GAAGGTTTTG 
GGAACAGTAA AATGGTTCAA TGTAAGGAAC GGATATGGTT TCATCAACAG GAATGACACC 
AAGGAAGATG TATTTGTACA CCAGACTGCC ATAAAGAAGA ATAACCCCAG GAAGTACCTT 
CGCAGTGTAG GAGATGGAGA GACTGTGGAG TTTGATGTTG TTGAAGGAGA AAAGGGTGCG 
GAGGCAGCAA ATGTTACAGG TCCTGGTGGA GTTCCAGTGC AAGGCAGTAA ATATGCAGCA 
GACCGTAACC ATTATAGACG CTACCCACGT CGTAGGGGTC CTCCACGCAA TTACCAGCAG 
AATTACCAGA ATAGTGAGAG TGGGGAAAAG AATGAGGGAT CGGAGAGCGC TCCCGAAGGC 
CAGGCCCAAC AACGCCGGCC CTACCGCAGG CGAAGGTTCC CACCTTACTA CATGCGGAGA 
CCCTATGGGC GTCGACCACA GTATTCCAAC CCTCCTGTGC AGGGAGAAGT GATGGAAGGT 
GCTGACAACC AGGGTGCAGG AGAACAAGGT AGACCAGTGA GACAGAATAT GTATCGGGGT 
TATAGACCAC GATTCCGCAG AGGTCCTCCT CGCCAAAGAC AGCCTAGAGA GGACGGCAAT 
GAAGAAGACA AGGAAAACCA AGGAGATGAG ACCCAGGGTC AGCAGCCACC TCCGAGTCGG 
TACCGCCGCA ACTTCAACTA CCGACGCAGA CGCCCAGATA ACCCTAAACC ACAAGATGGC 
AAAGAGACAA AAGCAGCCGA TCCACCAGCT GAGAATTCGT CCGCTCCCGA GGCTGAGCAG 
GGCGGGGCTG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076254.1
CDS	104..1078
Translation	MSSEAETQQPPAAPPAAPALSAAETKPGTTGSGAGSGGPGGLTSAAPAGGDKKVIATKVLGTVKWFNVRNGYGFINRNDTKEDVFVHQTAIKKNNPRKYLRSVGDGETVEFDVVEGEKGAEAANVTGPGGVPVQGSKYAADRNHYRRYPRRRGPPRNYQQNYQNSESGEKNEGSESAPEGQAQQRRPYRRRRFPPYYMRRPYGRRPQYSNPPVQGEVMEGADNQGAGEQGRPVRQNMYRGYRPRFRRGPPRQRQPREDGNEEDKENQGDETQGQQPPPSRYRRNFNYRRRRPDNPKPQDGKETKAADPPAENSSAPEAEQGGAE

Target ORF information:

RefSeq Version	NM_001082785.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus Y box binding protein 1 (YBX1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001082785.1

ORF Insert Sequence:

ATGAGCAGCG AGGCCGAGAC CCAGCAGCCG CCCGCCGCCC CCCCCGCCGC CCCCGCCCTC 
AGCGCCGCCG AAACCAAGCC CGGCACCACG GGCAGCGGCG CAGGGAGCGG TGGCCCGGGC 
GGCCTCACAT CGGCGGCGCC TGCCGGCGGG GACAAGAAGG TCATCGCAAC GAAGGTTTTG 
GGAACAGTAA AATGGTTCAA TGTAAGGAAC GGATATGGTT TCATCAACAG GAATGACACC 
AAGGAAGATG TATTTGTACA CCAGACTGCC ATAAAGAAGA ATAACCCCAG GAAGTACCTT 
CGCAGTGTAG GAGATGGAGA GACTGTGGAG TTTGATGTTG TTGAAGGAGA AAAGGGTGCG 
GAGGCAGCAA ATGTTACAGG TCCTGGTGGA GTTCCAGTGC AAGGCAGTAA ATATGCAGCA 
GACCGTAACC ATTATAGACG CTACCCACGT CGTAGGGGTC CTCCACGCAA TTACCAGCAG 
AATTACCAGA ATAGTGAGAG TGGGGAAAAG AATGAGGGAT CGGAGAGCGC TCCCGAAGGC 
CAGGCCCAAC AACGCCGGCC CTACCGCAGG CGAAGGTTCC CACCTTACTA CATGCGGAGA 
CCCTATGGGC GTCGACCACA GTATTCCAAC CCTCCTGTGC AGGGAGAAGT GATGGAAGGT 
GCTGACAACC AGGGTGCAGG AGAACAAGGT AGACCAGTGA GACAGAATAT GTATCGGGGT 
TATAGACCAC GATTCCGCAG AGGTCCTCCT CGCCAAAGAC AGCCTAGAGA GGACGGCAAT 
GAAGAAGACA AGGAAAACCA AGGAGATGAG ACCCAGGGTC AGCAGCCACC TCCGAGTCGG 
TACCGCCGCA ACTTCAACTA CCGACGCAGA CGCCCAGATA ACCCTAAACC ACAAGATGGC 
AAAGAGACAA AAGCAGCCGA TCCACCAGCT GAGAATTCGT CCGCTCCCGA GGCTGAGCAG 
GGCGGGGCTG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

YB-1 synthesis is regulated by mTOR signaling pathway.
PloS one7(12)e52527(2012)
Lyabin DN,Eliseeva IA,Ovchinnikov LP

Interplay between Y-box-binding protein 1 (YB-1) and poly(A) binding protein (PABP) in specific regulation of YB-1 mRNA translation.
RNA biology8(5)883-92(2011 Sep-Oct)
Lyabin DN,Eliseeva IA,Skabkina OV,Ovchinnikov LP

Akt-mediated YB-1 phosphorylation activates translation of silent mRNA species.
Molecular and cellular biology26(1)277-92(2006 Jan)
Evdokimova V,Ruzanov P,Anglesio MS,Sorokin AV,Ovchinnikov LP,Buckley J,Triche TJ,Sonenberg N,Sorensen PH

Proteasome-mediated cleavage of the Y-box-binding protein 1 is linked to DNA-damage stress response.
The EMBO journal24(20)3602-12(2005 Oct)
Sorokin AV,Selyutina AA,Skabkin MA,Guryanov SG,Nazimov IV,Richard C,Th'ng J,Yau J,Sorensen PH,Ovchinnikov LP,Evdokimova V

The mRNA-binding protein YB-1 (p50) prevents association of the eukaryotic initiation factor eIF4G with mRNA and inhibits protein synthesis at the initiation stage.
The Journal of biological chemistry278(16)13936-43(2003 Apr)
Nekrasov MP,Ivshina MP,Chernov KG,Kovrigina EA,Evdokimova VM,Thomas AA,Hershey JW,Ovchinnikov LP