SPG11 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017022635.1	XP_016878124.1	spatacsin isoform X3
NM_001160227.1	NP_001153699.1	spatacsin isoform 2
XM_017022635.2	XP_016878124.1	spatacsin isoform X3
XM_017022636.1	XP_016878125.1	spatacsin isoform X5
XM_006720700.1	XP_006720763.1	spatacsin isoform X2
NM_001160227.2	NP_001153699.1	spatacsin isoform 2
XM_006720701.3	XP_006720764.1	spatacsin isoform X4
NM_025137.4	NP_079413.3	spatacsin isoform 1
XM_017022634.1	XP_016878123.1	spatacsin isoform X1
NM_025137.3	NP_079413.3	spatacsin isoform 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu85698	XM_017022636.1 Latest version!	Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu85697	XM_017022635.2 Latest version!	Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu48597	XM_006720701.3 Latest version!	Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu85696	XM_017022634.1 Latest version!	Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu16493	NM_001160227.2 Latest version!	Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu19157	NM_025137.4 Latest version!	Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu48595	XM_006720700.1 Latest version!	Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
View Old Accession Versions >>
OHu85697	XM_017022635.1	Homo sapiens spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu16493	NM_001160227.1	Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu19157	NM_025137.3	Homo sapiens SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
Hide Old Accession Versions >>

SPG11 ( XM_017022636.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878125.1 Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu85698
Clone ID Related Accession (Same CDS sequence)	XM_017022636.1
Accession Version	XM_017022636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spatacsin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACCT TGACTGTTAC AAATCCCAAA CTGATCTTCC AGGCTAGCCT TGCAAATGCT 
CAGATTTTGA TTCCCACCAA TCAGGCCAGT GTAAGCAGTA TGCTATTGGA AGGACATACC 
CTCCTGGCCC TTGCTACTAC AATGTATTCT CCTGGGGGTG TCAGTCAGGT TGTTCAGAAT 
GAAGAAAATG AAAACTGTTT GAAGAAAGTG GATCCCCAGC TATTGAAGAT GGCATTAACT 
CCTTACCCCA AGCTAAAAAC TGCTCTCTTC CCACAGTGCA CTCCTCCTAG TGTCCTGCCA 
TCTGATATTA CAATCTACCA CCTTATTCAG TCATTATCAC CCTTTGATCC TAGCAGATTG 
TTTGGCTGGC AGTCTGCTAA CACACTAGCT ATAGGAGATG CATGGAGTCA TCTCCCACAT 
TTCTCTAGCC CTGACCTGGT TAATAAATAT GCTATAGTGG AACGTCTGAA TTTTGCTTAT 
TATTTACATA ATGGGCGGCC ATCATTTGCA TTTGGTACTT TTCTGGTCCA GGAATTAATC 
AAGAGCAAGA CTCCCAAGCA GCTGATCCAG CAAGTAGGCA ATGAAGCCTA TGTTATAGGG 
CTCTCCTCCT TCCACATACC TTCAATAGGA GCTGCATGTG TTTGTTTCTT AGAATTGCTT 
GGCCTTGACA GCCTCAAGCT CAGAGTTGAT ATGAAAGTGG CCAATATAAT TTTGAGCTAC 
AAGTGCAGAA ATGAAGATGC TCAGTACAGC TTTATCAGAG AGTCTGTAGC CGAAAAACTA 
TCTAAACTAG CTGATGGTGA AAAGACAACC ACAGAAGAAT TGCTTGTTCT CTTAGAAGAA 
GGTACATGGA ACAGCATTCA GCAACAGGAA ATAAAGAGGT TATCCAGTGA ATCTAGCAGC 
CAATGGGCAT TAGTGGTGCA GTTCTGCAGG CTACACAATA TGAAACTAAG CATATCTTAC 
CTTAGAGAAT GTGCCAAAGC AAATGATTGG CTGCAGTTCA TTATTCACAG CCAACTCCAC 
AACTACCACC CAGCAGAGGT GAAATCCCTT ATCCAGTACT TCAGCCCAGT CATTCAAGAC 
CACTTAAGGC TGGCTTTTGA GAACTTGCCC TCAGTGCCCA CCTCCAAAAT GGACAGCGAT 
CAAGTCTGCA ATAAGTGCCC CCAGGAACTT CAAGGAAGCA AACAAGAGAT GACCGATTTA 
TTTGAAATTC TGCTCCAATG CTCAGAGGAG CCAGACTCCT GGCACTGGCT TCTGGTTGAA 
GCAGTGAAAC AACAGGCCCC TATCCTCAGT GTTCTGGCCT CATGTCTCCA GGGTGCCAGT 
GCCATTTCTT GTCTCTGTGT TTGGATCATC ACTTCTGTGG AGGACAATGT TGCAACTGAA 
GCAATGGGAC ACATTCAGGA CTCAACAGAG GACCATACCT GGAACCTTGA GGATCTTTCA 
GTCATCTGGA GAACATTATT AACAAGACAA AAGAGCAAAA CTCTCATCAG AGGTTTCCAG 
CTTTTCTTTA AGGATTCCCC GTTACTACTG GTGATGGAGA TGTATGAACT GTGTATGTTC 
TTCAGGAATT ATAAAGAAGC TGAAGCTAAA CTTCTGGAGT TTCAGAAGAG CCTTGAAACG 
CTTAACACAG CAGCCACAAA GGTCCACCCT GTCATCCCTG CCATGTGGCT GGAGGATCAG 
GTGTGTTTCC TTTTGAAGCT TATGCTACAG CAGTGTAAGA CCCAGTATGA GCTGGGGAAG 
CTTTTACAGC TCTTTGTTGA AAGAGAGCAT CTCTTCTCTG ATGGTCCAGA TGTGAAAAAG 
CTTTGCATCC TTTGCCAGAT TTTGAAGGAT ACATCCATAG CCATTAATCA TACAATTATT 
ACCAGCTACA GCATTGAGAA TCTTCAGCAT GAATGTAGAT CTATTTTGGA AAGACTGCAG 
ACAGATGGAC AATTCGCTTT GGCCAGGAGG GTAGCAGAAT TAGCTGAGTT ACCTGTGGAC 
AACTTGGTTA TTAAAGAGAT AACACAGGAA ATGCAGACCC TAAAACACAT TGAACAGTGG 
TCACTAAAAC AAGCAAGAAT TGACTTCTGG AAAAAATGCC ATGAGAATTT TAAGAAAAAT 
TCAATTTCAA GCAAAGCAGC TTCTTCCTTT TTCTCAACCC AGGCCCATGT GGCATGTGAG 
CACCCAACTG GATGGAGCAG CATGGAGGAG CGCCATCTGC TGCTCACCTT GGCAGGGCAC 
TGGCTTGCCC AGGAGGACGT GGTGCCCTTG GATAAGCTGG AGGAGCTGGA GAAGCAGATC 
TGGCTGTGCC GCATCACCCA GCACACTCTT GGAAGAAATC AGGAGGAAAC AGAGCCCAGA 
TTTTCTCGAC AGATCTCAAC TAGTGGTGAA CTTTCCTTTG ATAGTTTAGC CAGTGAGTTT 
TCCTTCTCCA AGTTGGCTGC TCTGAACACA TCAAAATACT TAGAACTTAA CAGCCTTCCA 
TCCAAAGAGA CATGCGAGAA TAGATTGGAT TGGAAAGAGC AGGAGTCACT AAACTTTTTG 
ATTGGGCGCC TACTGGATGA TGGCTGTGTG CATGAAGCAA GTAGAGTATG CCGGTATTTT 
CATTTTTATA ATCCAGATGT CGCCTTGGTA TTGCACTGCA GAGCACTGGC CTCAGGGGAA 
GCTAGTATGG AGGATCTGCA CCCAGAGATC CATGCTCTCC TACAAAGTGC TGAGCTGCTT 
GAGGAAGAAG CACCCGACAT TCCCCTAAGG AGAGTCCACA GCACTTCAAG TCTGGATAGT 
CAGAAGTTTG TGACAGTGCC CTCCAGTAAT GAAGTGGTAA CTAACCTGGA AGTGCTGACA 
AGCAAATGCC TCCATGGGAA GAACTACTGT CGACAGGTCC TCTGTCTGTA TGATCTTGCC 
AAGGAGTTGG GCTGTTCCTA CACAGATGTT GCTGCTCAGG ATGGTGAAGC CATGCTCCGG 
AAAATCTTGG CCTCTCAGCA GCCTGACCGA TGCAAACGAG CCCAGGCCTT CATCAGCACA 
CAGGGCCTTA AGCCAGATAC TGTGGCTGAA CTCGTGGCAG AAGAGGTGAC ACGGGAGCTG 
CTTACTTCAT CACAGGGAAC AGGACATAAG CAGATGTTCA ACCCAACAGA GGAAAGCCAG 
ACATTTCTTC AGCTGACCAC TCTGTGTCAA GACCGCACAT TGGTAGGCAT GAAGTTGTTG 
GATAAGATTT CCTCCGTTCC CCATGGGGAA CTGTCTTGCA CCACAGAGCT CCTGATCCTG 
GCCCATCATT GCTTCACCCT GACGTGCCAC ATGGAGGGCA TCATCCGAGT CCTACAGGCC 
GCCCACATGC TCACAGATAA CCACCTGGCC CCCAGTGAGG AGTATGGGCT GGTGGTACGG 
CTCCTCACTG GCATTGGAAG GTACAACGAG ATGACATACA TATTTGATTT GCTGCATAAA 
AAGCACTACT TTGAAGTGCT AATGAGGAAG AAGTTGGATC CGAGTGGTAC CCTGAAAACA 
GCCCTGCTGG ACTACATCAA ACGCTGCCGT CCTGGAGACA GTGAAAAGCA CAATATGATT 
GCCCTGTGCT TCAGCATGTG CCGGGAGATT GGCGAGAACC ACGAGGCAGC TGCCCGCATC 
CAACTGAAAT TGATTGAGTC TCAGCCCTGG GAGGACAGCC TCAAGGATGG GCACCAGCTG 
AAACAACTGC TGCTGAAGGC CCTGACTCTG ATGTTGGATG CAGCAGAGAG TTATGCCAAG 
GACTCCTGTG TGCGACAGGC CCAGCACTGT CAGCGGCTCA CCAAGTTGAT AACTCTGCAG 
ATTCACTTTC TGAACACTGG CCAGAACACA ATGCTCATCA ACTTGGGCCG CCACAAGCTG 
ATGGACTGTA TTCTGGCCCT ACCTCGGTTC TACCAGGCTT CTATTGTGGC TGAGGCCTAC 
GATTTTGTTC CAGATTGGGC TGAAATTTTA TACCAGCAAG TGATTCTTAA AGGAGACTTT 
AATTACTTGG AAGAATTTAA GCAGCAAAGG TTATTAAAGT CCAGTATATT TGAAGAGATT 
TCCAAAAAAT ATAAACAACA TCAGCCTACT GACATGGTCA TGGAAAACCT GAAGAAATTA 
CTCACATATT GTGAAGATGT TTACCTGTAT TACAAGTTGG CATACGAACA CAAGTTTTAT 
GAAATTGTAA ATGTGCTTCT GAAGGACCCT CAGACAGGTT GCTGTCTAAA GGACATGCTA 
GCAGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878125.1
CDS	964..5172
Translation	MSTLTVTNPKLIFQASLANAQILIPTNQASVSSMLLEGHTLLALATTMYSPGGVSQVVQNEENENCLKKVDPQLLKMALTPYPKLKTALFPQCTPPSVLPSDITIYHLIQSLSPFDPSRLFGWQSANTLAIGDAWSHLPHFSSPDLVNKYAIVERLNFAYYLHNGRPSFAFGTFLVQELIKSKTPKQLIQQVGNEAYVIGLSSFHIPSIGAACVCFLELLGLDSLKLRVDMKVANIILSYKCRNEDAQYSFIRESVAEKLSKLADGEKTTTEELLVLLEEGTWNSIQQQEIKRLSSESSSQWALVVQFCRLHNMKLSISYLRECAKANDWLQFIIHSQLHNYHPAEVKSLIQYFSPVIQDHLRLAFENLPSVPTSKMDSDQVCNKCPQELQGSKQEMTDLFEILLQCSEEPDSWHWLLVEAVKQQAPILSVLASCLQGASAISCLCVWIITSVEDNVATEAMGHIQDSTEDHTWNLEDLSVIWRTLLTRQKSKTLIRGFQLFFKDSPLLLVMEMYELCMFFRNYKEAEAKLLEFQKSLETLNTAATKVHPVIPAMWLEDQVCFLLKLMLQQCKTQYELGKLLQLFVEREHLFSDGPDVKKLCILCQILKDTSIAINHTIITSYSIENLQHECRSILERLQTDGQFALARRVAELAELPVDNLVIKEITQEMQTLKHIEQWSLKQARIDFWKKCHENFKKNSISSKAASSFFSTQAHVACEHPTGWSSMEERHLLLTLAGHWLAQEDVVPLDKLEELEKQIWLCRITQHTLGRNQEETEPRFSRQISTSGELSFDSLASEFSFSKLAALNTSKYLELNSLPSKETCENRLDWKEQESLNFLIGRLLDDGCVHEASRVCRYFHFYNPDVALVLHCRALASGEASMEDLHPEIHALLQSAELLEEEAPDIPLRRVHSTSSLDSQKFVTVPSSNEVVTNLEVLTSKCLHGKNYCRQVLCLYDLAKELGCSYTDVAAQDGEAMLRKILASQQPDRCKRAQAFISTQGLKPDTVAELVAEEVTRELLTSSQGTGHKQMFNPTEESQTFLQLTTLCQDRTLVGMKLLDKISSVPHGELSCTTELLILAHHCFTLTCHMEGIIRVLQAAHMLTDNHLAPSEEYGLVVRLLTGIGRYNEMTYIFDLLHKKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAARIQLKLIESQPWEDSLKDGHQLKQLLLKALTLMLDAAESYAKDSCVRQAQHCQRLTKLITLQIHFLNTGQNTMLINLGRHKLMDCILALPRFYQASIVAEAYDFVPDWAEILYQQVILKGDFNYLEEFKQQRLLKSSIFEEISKKYKQHQPTDMVMENLKKLLTYCEDVYLYYKLAYEHKFYEIVNVLLKDPQTGCCLKDMLAG

Target ORF information:

RefSeq Version	XM_017022636.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022636.1

ORF Insert Sequence:

ATGTCTACCT TGACTGTTAC AAATCCCAAA CTGATCTTCC AGGCTAGCCT TGCAAATGCT 
CAGATTTTGA TTCCCACCAA TCAGGCCAGT GTAAGCAGTA TGCTATTGGA AGGACATACC 
CTCCTGGCCC TTGCTACTAC AATGTATTCT CCTGGGGGTG TCAGTCAGGT TGTTCAGAAT 
GAAGAAAATG AAAACTGTTT GAAGAAAGTG GATCCCCAGC TATTGAAGAT GGCATTAACT 
CCTTACCCCA AGCTAAAAAC TGCTCTCTTC CCACAGTGCA CTCCTCCTAG TGTCCTGCCA 
TCTGATATTA CAATCTACCA CCTTATTCAG TCATTATCAC CCTTTGATCC TAGCAGATTG 
TTTGGCTGGC AGTCTGCTAA CACACTAGCT ATAGGAGATG CATGGAGTCA TCTCCCACAT 
TTCTCTAGCC CTGACCTGGT TAATAAATAT GCTATAGTGG AACGTCTGAA TTTTGCTTAT 
TATTTACATA ATGGGCGGCC ATCATTTGCA TTTGGTACTT TTCTGGTCCA GGAATTAATC 
AAGAGCAAGA CTCCCAAGCA GCTGATCCAG CAAGTAGGCA ATGAAGCCTA TGTTATAGGG 
CTCTCCTCCT TCCACATACC TTCAATAGGA GCTGCATGTG TTTGTTTCTT AGAATTGCTT 
GGCCTTGACA GCCTCAAGCT CAGAGTTGAT ATGAAAGTGG CCAATATAAT TTTGAGCTAC 
AAGTGCAGAA ATGAAGATGC TCAGTACAGC TTTATCAGAG AGTCTGTAGC CGAAAAACTA 
TCTAAACTAG CTGATGGTGA AAAGACAACC ACAGAAGAAT TGCTTGTTCT CTTAGAAGAA 
GGTACATGGA ACAGCATTCA GCAACAGGAA ATAAAGAGGT TATCCAGTGA ATCTAGCAGC 
CAATGGGCAT TAGTGGTGCA GTTCTGCAGG CTACACAATA TGAAACTAAG CATATCTTAC 
CTTAGAGAAT GTGCCAAAGC AAATGATTGG CTGCAGTTCA TTATTCACAG CCAACTCCAC 
AACTACCACC CAGCAGAGGT GAAATCCCTT ATCCAGTACT TCAGCCCAGT CATTCAAGAC 
CACTTAAGGC TGGCTTTTGA GAACTTGCCC TCAGTGCCCA CCTCCAAAAT GGACAGCGAT 
CAAGTCTGCA ATAAGTGCCC CCAGGAACTT CAAGGAAGCA AACAAGAGAT GACCGATTTA 
TTTGAAATTC TGCTCCAATG CTCAGAGGAG CCAGACTCCT GGCACTGGCT TCTGGTTGAA 
GCAGTGAAAC AACAGGCCCC TATCCTCAGT GTTCTGGCCT CATGTCTCCA GGGTGCCAGT 
GCCATTTCTT GTCTCTGTGT TTGGATCATC ACTTCTGTGG AGGACAATGT TGCAACTGAA 
GCAATGGGAC ACATTCAGGA CTCAACAGAG GACCATACCT GGAACCTTGA GGATCTTTCA 
GTCATCTGGA GAACATTATT AACAAGACAA AAGAGCAAAA CTCTCATCAG AGGTTTCCAG 
CTTTTCTTTA AGGATTCCCC GTTACTACTG GTGATGGAGA TGTATGAACT GTGTATGTTC 
TTCAGGAATT ATAAAGAAGC TGAAGCTAAA CTTCTGGAGT TTCAGAAGAG CCTTGAAACG 
CTTAACACAG CAGCCACAAA GGTCCACCCT GTCATCCCTG CCATGTGGCT GGAGGATCAG 
GTGTGTTTCC TTTTGAAGCT TATGCTACAG CAGTGTAAGA CCCAGTATGA GCTGGGGAAG 
CTTTTACAGC TCTTTGTTGA AAGAGAGCAT CTCTTCTCTG ATGGTCCAGA TGTGAAAAAG 
CTTTGCATCC TTTGCCAGAT TTTGAAGGAT ACATCCATAG CCATTAATCA TACAATTATT 
ACCAGCTACA GCATTGAGAA TCTTCAGCAT GAATGTAGAT CTATTTTGGA AAGACTGCAG 
ACAGATGGAC AATTCGCTTT GGCCAGGAGG GTAGCAGAAT TAGCTGAGTT ACCTGTGGAC 
AACTTGGTTA TTAAAGAGAT AACACAGGAA ATGCAGACCC TAAAACACAT TGAACAGTGG 
TCACTAAAAC AAGCAAGAAT TGACTTCTGG AAAAAATGCC ATGAGAATTT TAAGAAAAAT 
TCAATTTCAA GCAAAGCAGC TTCTTCCTTT TTCTCAACCC AGGCCCATGT GGCATGTGAG 
CACCCAACTG GATGGAGCAG CATGGAGGAG CGCCATCTGC TGCTCACCTT GGCAGGGCAC 
TGGCTTGCCC AGGAGGACGT GGTGCCCTTG GATAAGCTGG AGGAGCTGGA GAAGCAGATC 
TGGCTGTGCC GCATCACCCA GCACACTCTT GGAAGAAATC AGGAGGAAAC AGAGCCCAGA 
TTTTCTCGAC AGATCTCAAC TAGTGGTGAA CTTTCCTTTG ATAGTTTAGC CAGTGAGTTT 
TCCTTCTCCA AGTTGGCTGC TCTGAACACA TCAAAATACT TAGAACTTAA CAGCCTTCCA 
TCCAAAGAGA CATGCGAGAA TAGATTGGAT TGGAAAGAGC AGGAGTCACT AAACTTTTTG 
ATTGGGCGCC TACTGGATGA TGGCTGTGTG CATGAAGCAA GTAGAGTATG CCGGTATTTT 
CATTTTTATA ATCCAGATGT CGCCTTGGTA TTGCACTGCA GAGCACTGGC CTCAGGGGAA 
GCTAGTATGG AGGATCTGCA CCCAGAGATC CATGCTCTCC TACAAAGTGC TGAGCTGCTT 
GAGGAAGAAG CACCCGACAT TCCCCTAAGG AGAGTCCACA GCACTTCAAG TCTGGATAGT 
CAGAAGTTTG TGACAGTGCC CTCCAGTAAT GAAGTGGTAA CTAACCTGGA AGTGCTGACA 
AGCAAATGCC TCCATGGGAA GAACTACTGT CGACAGGTCC TCTGTCTGTA TGATCTTGCC 
AAGGAGTTGG GCTGTTCCTA CACAGATGTT GCTGCTCAGG ATGGTGAAGC CATGCTCCGG 
AAAATCTTGG CCTCTCAGCA GCCTGACCGA TGCAAACGAG CCCAGGCCTT CATCAGCACA 
CAGGGCCTTA AGCCAGATAC TGTGGCTGAA CTCGTGGCAG AAGAGGTGAC ACGGGAGCTG 
CTTACTTCAT CACAGGGAAC AGGACATAAG CAGATGTTCA ACCCAACAGA GGAAAGCCAG 
ACATTTCTTC AGCTGACCAC TCTGTGTCAA GACCGCACAT TGGTAGGCAT GAAGTTGTTG 
GATAAGATTT CCTCCGTTCC CCATGGGGAA CTGTCTTGCA CCACAGAGCT CCTGATCCTG 
GCCCATCATT GCTTCACCCT GACGTGCCAC ATGGAGGGCA TCATCCGAGT CCTACAGGCC 
GCCCACATGC TCACAGATAA CCACCTGGCC CCCAGTGAGG AGTATGGGCT GGTGGTACGG 
CTCCTCACTG GCATTGGAAG GTACAACGAG ATGACATACA TATTTGATTT GCTGCATAAA 
AAGCACTACT TTGAAGTGCT AATGAGGAAG AAGTTGGATC CGAGTGGTAC CCTGAAAACA 
GCCCTGCTGG ACTACATCAA ACGCTGCCGT CCTGGAGACA GTGAAAAGCA CAATATGATT 
GCCCTGTGCT TCAGCATGTG CCGGGAGATT GGCGAGAACC ACGAGGCAGC TGCCCGCATC 
CAACTGAAAT TGATTGAGTC TCAGCCCTGG GAGGACAGCC TCAAGGATGG GCACCAGCTG 
AAACAACTGC TGCTGAAGGC CCTGACTCTG ATGTTGGATG CAGCAGAGAG TTATGCCAAG 
GACTCCTGTG TGCGACAGGC CCAGCACTGT CAGCGGCTCA CCAAGTTGAT AACTCTGCAG 
ATTCACTTTC TGAACACTGG CCAGAACACA ATGCTCATCA ACTTGGGCCG CCACAAGCTG 
ATGGACTGTA TTCTGGCCCT ACCTCGGTTC TACCAGGCTT CTATTGTGGC TGAGGCCTAC 
GATTTTGTTC CAGATTGGGC TGAAATTTTA TACCAGCAAG TGATTCTTAA AGGAGACTTT 
AATTACTTGG AAGAATTTAA GCAGCAAAGG TTATTAAAGT CCAGTATATT TGAAGAGATT 
TCCAAAAAAT ATAAACAACA TCAGCCTACT GACATGGTCA TGGAAAACCT GAAGAAATTA 
CTCACATATT GTGAAGATGT TTACCTGTAT TACAAGTTGG CATACGAACA CAAGTTTTAT 
GAAATTGTAA ATGTGCTTCT GAAGGACCCT CAGACAGGTT GCTGTCTAAA GGACATGCTA 
GCAGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_017022635.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878124.1 Homo sapiens spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu85697
Clone ID Related Accession (Same CDS sequence)	XM_017022635.1 , XM_017022635.2
Accession Version	XM_017022635.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	spatacsin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG 
CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG 
GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG 
GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA 
AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG 
AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG 
CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC 
AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA 
AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG 
GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC 
TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT 
CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT 
TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA 
GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA 
ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC 
TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC 
CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT 
CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT 
GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG 
AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA 
CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT 
TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG 
CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG 
TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG 
TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC 
AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT 
CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA 
AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG 
GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA 
CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC 
CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG 
AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG 
CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC 
AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA 
ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG 
CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA 
GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC 
TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT 
TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT 
TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA 
CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT 
GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA 
ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT 
GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT 
GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG 
ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA 
GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC 
CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC 
AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT 
CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC 
TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT 
CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT 
GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT 
ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG 
AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA 
ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG 
CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA 
TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA 
CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT 
TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA 
ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA 
GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG 
CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC 
TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA 
CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA 
GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC 
AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT 
TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC 
CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA 
GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC 
GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT 
TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT 
GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC 
AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT 
GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT 
TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC 
CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG 
TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA 
ACGTCTAGAG TGAACTACGA GACTATAGTG ACTGAGAACC AAATATTAGT CTTCCAAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878124.1
CDS	32..4834
Translation	MAAEEGVASAASAGGSWGTAAMGRVLPMLLVPVPAEAMGQLGSRAQLRTQPEALGSLTAAGSLQVLSLTPGSRGGGRCCLEGPFWHFLWEDSRNSSTPTEKPKLLALGENYELLIYEFNLKDGRCDATILYSCSREALQKLIDDQDISISLLSLRILSFHNNTSLLFINKCVILHIIFPERDAAIRVLNCFTLPLPAQAVDMIIDTQLCRGILFVLSSLGWIYIFDVVDGTYVAHVDLALHKEDMCNEQQQEPAKISSFTSLKVSQDLDVAVIVSSSNSAVALNLNLYFRQHPGHLLCERILEDLPIQGPKGVDEDDPVNSAYNMKLAKFSFQIDRSWKAQLSSLNETIKNSKLEVSCCAPWFQDILHLESPESGNHSTSVQSWAFIPQDIMHGQYNVLQKDHAKTSDPGRSWKIMHISEQEEPIELKCVSVTGFTALFTWEVERMGYTITLWDLETQGMQCFSLGTKCIPVDSSGDQQLCFVLTENGLSLILFGLTQEEFLNRLMIHGSASTVDTLCHLNGWGRCSIPIHALEAGIENRQLDTVNFFLKSKENLFNPSSKSSVSDQFDHLSSHLYLRNVEELIPALDLLCSAIRESYSEPQSKHFSEQLLNLTLSFLNNQIKELFIHTEELDEHLQKGVNILTSYINELRTFMIKFPWKLTDAIDEYDVHENVPKVKESNIWKKLSFEEVIASAILNNKIPEAQTFFRIDSHSAQKLEELIGIGLNLVFDNLKKNNIKEASELLKNMGFDVKGQLLKICFYTTNKNIRDFLVEILKEKNYFSEKEKRTIDFVHQVEKLYLGHFQENMQIQSFPRYWIKEQDFFKHKSVLDSFLKYDCKDEFNKQDHRIVLNWALWWDQLTQESILLPRISPEEYKSYSPEALWRYLTARHDWLNIILWIGEFQTQHSYASLQQNKWPLLTVDVINQNTSCNNYMRNEILDKLARNGVFLASELEDFECFLLRLSRIGGVIQDTLPVQNYKTKEGWDFHSQFILYCLEHSLQHLLYVYLDCYKLSPENCPFLEKKELHEAHPWFEFLVQCRQVASNLTDPKLIFQASLANAQILIPTNQASVSSMLLEGHTLLALATTMYSPGGVSQVVQNEENENCLKKVDPQLLKMALTPYPKLKTALFPQCTPPSVLPSDITIYHLIQSLSPFDPSRLFGWQSANTLAIGDAWSHLPHFSSPDLVNKYAIVERLNFAYYLHNGRPSFAFGTFLVQELIKSKTPKQLIQQVGNEAYVIGLSSFHIPSIGAACVCFLELLGLDSLKLRVDMKVANIILSYKCRNEDAQYSFIRESVAEKLSKLADGEKTTTEELLVLLEEGTWNSIQQQEIKRLSSESSSQWALVVQFCRLHNMKLSISYLRECAKANDWLQFIIHSQLHNYHPAEVKSLIQYFSPVIQDHLRLAFENLPSVPTSKMDSDQVCNKCPQELQGSKQEMTDLFEILLQCSEEPDSWHWLLVEAVKQQAPILSVLASCLQGASAISCLCVWIITSVEDNVATEAMGHIQDSTEDHTWNLEDLSVIWRTLLTRQKSKTLIRGFQLFFKDSPLLLVMEMYELCMFFRNYKEAEAKLLEFQKSLETSRVNYETIVTENQILVFQK

Target ORF information:

RefSeq Version	XM_017022635.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spastic paraplegia 11 (autosomal recessive) (SPG11), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022635.1

ORF Insert Sequence:

ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG 
CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG 
GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG 
GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA 
AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG 
AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG 
CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC 
AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA 
AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG 
GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC 
TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT 
CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT 
TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA 
GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA 
ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC 
TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC 
CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT 
CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT 
GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG 
AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA 
CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT 
TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG 
CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG 
TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG 
TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC 
AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT 
CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA 
AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG 
GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA 
CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC 
CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG 
AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG 
CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC 
AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA 
ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG 
CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA 
GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC 
TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT 
TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT 
TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA 
CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT 
GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA 
ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT 
GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT 
GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG 
ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA 
GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC 
CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC 
AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT 
CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC 
TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT 
CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT 
GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT 
ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG 
AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA 
ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG 
CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA 
TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA 
CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT 
TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA 
ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA 
GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG 
CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC 
TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA 
CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA 
GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC 
AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT 
TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC 
CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA 
GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC 
GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT 
TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT 
GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC 
AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT 
GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT 
TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC 
CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG 
TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA 
ACGTCTAGAG TGAACTACGA GACTATAGTG ACTGAGAACC AAATATTAGT CTTCCAAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_017022635.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878124.1 Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X3, mRNA.

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CloneID	OHu85697
Clone ID Related Accession (Same CDS sequence)	XM_017022635.1 , XM_017022635.2
Accession Version	XM_017022635.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spatacsin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017022635.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG 
CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG 
GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG 
GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA 
AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG 
AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG 
CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC 
AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA 
AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG 
GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC 
TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT 
CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT 
TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA 
GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA 
ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC 
TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC 
CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT 
CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT 
GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG 
AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA 
CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT 
TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG 
CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG 
TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG 
TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC 
AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT 
CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA 
AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG 
GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA 
CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC 
CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG 
AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG 
CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC 
AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA 
ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG 
CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA 
GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC 
TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT 
TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT 
TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA 
CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT 
GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA 
ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT 
GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT 
GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG 
ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA 
GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC 
CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC 
AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT 
CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC 
TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT 
CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT 
GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT 
ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG 
AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA 
ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG 
CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA 
TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA 
CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT 
TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA 
ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA 
GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG 
CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC 
TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA 
CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA 
GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC 
AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT 
TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC 
CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA 
GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC 
GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT 
TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT 
GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC 
AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT 
GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT 
TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC 
CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG 
TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA 
ACGTCTAGAG TGAACTACGA GACTATAGTG ACTGAGAACC AAATATTAGT CTTCCAAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878124.1
CDS	32..4834
Translation	MAAEEGVASAASAGGSWGTAAMGRVLPMLLVPVPAEAMGQLGSRAQLRTQPEALGSLTAAGSLQVLSLTPGSRGGGRCCLEGPFWHFLWEDSRNSSTPTEKPKLLALGENYELLIYEFNLKDGRCDATILYSCSREALQKLIDDQDISISLLSLRILSFHNNTSLLFINKCVILHIIFPERDAAIRVLNCFTLPLPAQAVDMIIDTQLCRGILFVLSSLGWIYIFDVVDGTYVAHVDLALHKEDMCNEQQQEPAKISSFTSLKVSQDLDVAVIVSSSNSAVALNLNLYFRQHPGHLLCERILEDLPIQGPKGVDEDDPVNSAYNMKLAKFSFQIDRSWKAQLSSLNETIKNSKLEVSCCAPWFQDILHLESPESGNHSTSVQSWAFIPQDIMHGQYNVLQKDHAKTSDPGRSWKIMHISEQEEPIELKCVSVTGFTALFTWEVERMGYTITLWDLETQGMQCFSLGTKCIPVDSSGDQQLCFVLTENGLSLILFGLTQEEFLNRLMIHGSASTVDTLCHLNGWGRCSIPIHALEAGIENRQLDTVNFFLKSKENLFNPSSKSSVSDQFDHLSSHLYLRNVEELIPALDLLCSAIRESYSEPQSKHFSEQLLNLTLSFLNNQIKELFIHTEELDEHLQKGVNILTSYINELRTFMIKFPWKLTDAIDEYDVHENVPKVKESNIWKKLSFEEVIASAILNNKIPEAQTFFRIDSHSAQKLEELIGIGLNLVFDNLKKNNIKEASELLKNMGFDVKGQLLKICFYTTNKNIRDFLVEILKEKNYFSEKEKRTIDFVHQVEKLYLGHFQENMQIQSFPRYWIKEQDFFKHKSVLDSFLKYDCKDEFNKQDHRIVLNWALWWDQLTQESILLPRISPEEYKSYSPEALWRYLTARHDWLNIILWIGEFQTQHSYASLQQNKWPLLTVDVINQNTSCNNYMRNEILDKLARNGVFLASELEDFECFLLRLSRIGGVIQDTLPVQNYKTKEGWDFHSQFILYCLEHSLQHLLYVYLDCYKLSPENCPFLEKKELHEAHPWFEFLVQCRQVASNLTDPKLIFQASLANAQILIPTNQASVSSMLLEGHTLLALATTMYSPGGVSQVVQNEENENCLKKVDPQLLKMALTPYPKLKTALFPQCTPPSVLPSDITIYHLIQSLSPFDPSRLFGWQSANTLAIGDAWSHLPHFSSPDLVNKYAIVERLNFAYYLHNGRPSFAFGTFLVQELIKSKTPKQLIQQVGNEAYVIGLSSFHIPSIGAACVCFLELLGLDSLKLRVDMKVANIILSYKCRNEDAQYSFIRESVAEKLSKLADGEKTTTEELLVLLEEGTWNSIQQQEIKRLSSESSSQWALVVQFCRLHNMKLSISYLRECAKANDWLQFIIHSQLHNYHPAEVKSLIQYFSPVIQDHLRLAFENLPSVPTSKMDSDQVCNKCPQELQGSKQEMTDLFEILLQCSEEPDSWHWLLVEAVKQQAPILSVLASCLQGASAISCLCVWIITSVEDNVATEAMGHIQDSTEDHTWNLEDLSVIWRTLLTRQKSKTLIRGFQLFFKDSPLLLVMEMYELCMFFRNYKEAEAKLLEFQKSLETSRVNYETIVTENQILVFQK

Target ORF information:

RefSeq Version	XM_017022635.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022635.2

ORF Insert Sequence:

ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG 
CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG 
GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG 
GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA 
AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG 
AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG 
CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC 
AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA 
AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG 
GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC 
TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT 
CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT 
TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA 
GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA 
ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC 
TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC 
CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT 
CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT 
GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG 
AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA 
CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT 
TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG 
CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG 
TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG 
TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC 
AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT 
CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA 
AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG 
GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA 
CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC 
CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG 
AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG 
CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC 
AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA 
ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG 
CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA 
GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC 
TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT 
TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT 
TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA 
CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT 
GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA 
ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT 
GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT 
GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG 
ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA 
GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC 
CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC 
AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT 
CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC 
TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT 
CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT 
GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT 
ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG 
AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA 
ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG 
CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA 
TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA 
CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT 
TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA 
ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA 
GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG 
CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC 
TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA 
CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA 
GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC 
AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT 
TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC 
CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA 
GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC 
GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT 
TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT 
GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC 
AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT 
GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT 
TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC 
CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG 
TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA 
ACGTCTAGAG TGAACTACGA GACTATAGTG ACTGAGAACC AAATATTAGT CTTCCAAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_006720701.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006720764.1 Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X4, mRNA.

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CloneID	OHu48597
Clone ID Related Accession (Same CDS sequence)	XM_006720701.3
Accession Version	XM_006720701.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spatacsin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006720701.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG 
CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG 
GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG 
GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA 
AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG 
AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG 
CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC 
AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA 
AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG 
GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC 
TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT 
CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT 
TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA 
GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA 
ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC 
TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC 
CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT 
CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT 
GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG 
AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA 
CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT 
TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG 
CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG 
TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG 
TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC 
AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT 
CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA 
AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG 
GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA 
CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC 
CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG 
AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG 
CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC 
AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA 
ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG 
CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA 
GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC 
TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT 
TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT 
TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA 
CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT 
GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA 
ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT 
GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT 
GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG 
ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA 
GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC 
CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC 
AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT 
CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC 
TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT 
CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT 
GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT 
ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG 
AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA 
ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG 
CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA 
TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA 
CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT 
TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA 
ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA 
GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG 
CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC 
TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA 
CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA 
GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC 
AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT 
TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC 
CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA 
GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC 
GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT 
TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT 
GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC 
AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT 
GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT 
TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC 
CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG 
TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA 
ACGGTCCAGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006720764.1
CDS	32..4786
Translation	MAAEEGVASAASAGGSWGTAAMGRVLPMLLVPVPAEAMGQLGSRAQLRTQPEALGSLTAAGSLQVLSLTPGSRGGGRCCLEGPFWHFLWEDSRNSSTPTEKPKLLALGENYELLIYEFNLKDGRCDATILYSCSREALQKLIDDQDISISLLSLRILSFHNNTSLLFINKCVILHIIFPERDAAIRVLNCFTLPLPAQAVDMIIDTQLCRGILFVLSSLGWIYIFDVVDGTYVAHVDLALHKEDMCNEQQQEPAKISSFTSLKVSQDLDVAVIVSSSNSAVALNLNLYFRQHPGHLLCERILEDLPIQGPKGVDEDDPVNSAYNMKLAKFSFQIDRSWKAQLSSLNETIKNSKLEVSCCAPWFQDILHLESPESGNHSTSVQSWAFIPQDIMHGQYNVLQKDHAKTSDPGRSWKIMHISEQEEPIELKCVSVTGFTALFTWEVERMGYTITLWDLETQGMQCFSLGTKCIPVDSSGDQQLCFVLTENGLSLILFGLTQEEFLNRLMIHGSASTVDTLCHLNGWGRCSIPIHALEAGIENRQLDTVNFFLKSKENLFNPSSKSSVSDQFDHLSSHLYLRNVEELIPALDLLCSAIRESYSEPQSKHFSEQLLNLTLSFLNNQIKELFIHTEELDEHLQKGVNILTSYINELRTFMIKFPWKLTDAIDEYDVHENVPKVKESNIWKKLSFEEVIASAILNNKIPEAQTFFRIDSHSAQKLEELIGIGLNLVFDNLKKNNIKEASELLKNMGFDVKGQLLKICFYTTNKNIRDFLVEILKEKNYFSEKEKRTIDFVHQVEKLYLGHFQENMQIQSFPRYWIKEQDFFKHKSVLDSFLKYDCKDEFNKQDHRIVLNWALWWDQLTQESILLPRISPEEYKSYSPEALWRYLTARHDWLNIILWIGEFQTQHSYASLQQNKWPLLTVDVINQNTSCNNYMRNEILDKLARNGVFLASELEDFECFLLRLSRIGGVIQDTLPVQNYKTKEGWDFHSQFILYCLEHSLQHLLYVYLDCYKLSPENCPFLEKKELHEAHPWFEFLVQCRQVASNLTDPKLIFQASLANAQILIPTNQASVSSMLLEGHTLLALATTMYSPGGVSQVVQNEENENCLKKVDPQLLKMALTPYPKLKTALFPQCTPPSVLPSDITIYHLIQSLSPFDPSRLFGWQSANTLAIGDAWSHLPHFSSPDLVNKYAIVERLNFAYYLHNGRPSFAFGTFLVQELIKSKTPKQLIQQVGNEAYVIGLSSFHIPSIGAACVCFLELLGLDSLKLRVDMKVANIILSYKCRNEDAQYSFIRESVAEKLSKLADGEKTTTEELLVLLEEGTWNSIQQQEIKRLSSESSSQWALVVQFCRLHNMKLSISYLRECAKANDWLQFIIHSQLHNYHPAEVKSLIQYFSPVIQDHLRLAFENLPSVPTSKMDSDQVCNKCPQELQGSKQEMTDLFEILLQCSEEPDSWHWLLVEAVKQQAPILSVLASCLQGASAISCLCVWIITSVEDNVATEAMGHIQDSTEDHTWNLEDLSVIWRTLLTRQKSKTLIRGFQLFFKDSPLLLVMEMYELCMFFRNYKEAEAKLLEFQKSLETVQM

Target ORF information:

RefSeq Version	XM_006720701.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006720701.3

ORF Insert Sequence:

ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG 
CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG 
GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG 
GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA 
AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG 
AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG 
CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC 
AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA 
AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG 
GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC 
TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT 
CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT 
TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA 
GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA 
ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC 
TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC 
CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT 
CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT 
GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG 
AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA 
CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT 
TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG 
CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG 
TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG 
TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC 
AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT 
CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA 
AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG 
GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA 
CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC 
CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG 
AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG 
CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC 
AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA 
ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG 
CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA 
GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC 
TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT 
TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT 
TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA 
CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT 
GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA 
ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT 
GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT 
GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG 
ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA 
GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC 
CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC 
AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT 
CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC 
TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT 
CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT 
GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT 
ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG 
AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA 
ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG 
CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA 
TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA 
CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT 
TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA 
ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA 
GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG 
CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC 
TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA 
CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA 
GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC 
AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT 
TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC 
CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA 
GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC 
GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT 
TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT 
GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC 
AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT 
GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT 
TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC 
CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG 
TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA 
ACGGTCCAGA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_017022634.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878123.1 Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X1, mRNA.

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CloneID	OHu85696
Clone ID Related Accession (Same CDS sequence)	XM_017022634.1
Accession Version	XM_017022634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spatacsin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG 
CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG 
GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG 
GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA 
AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG 
AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG 
CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC 
AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA 
AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG 
GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC 
TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT 
CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT 
TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA 
GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA 
ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC 
TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC 
CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT 
CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT 
GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG 
AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA 
CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT 
TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG 
CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG 
TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG 
TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC 
AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT 
CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA 
AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG 
GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA 
CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC 
CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG 
AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG 
CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC 
AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA 
ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG 
CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA 
GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC 
TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT 
TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT 
TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA 
CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT 
GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA 
ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT 
GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT 
GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG 
ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA 
GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC 
CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC 
AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT 
CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC 
TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT 
CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT 
GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT 
ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG 
AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA 
ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG 
CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA 
TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA 
CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT 
TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA 
ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA 
GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG 
CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC 
TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA 
CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA 
GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC 
AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT 
TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC 
CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA 
GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC 
GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT 
TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT 
GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC 
AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT 
GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT 
TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC 
CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG 
TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA 
ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT 
CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG 
AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA 
AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT 
ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG 
CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG 
GACAACTTGG TTATTAAAGA GATAACACAG GAAATGCAGA CCCTAAAACA CATTGAACAG 
TGGTCACTAA AACAAGCAAG AATTGACTTC TGGAAAAAAT GCCATGAGAA TTTTAAGAAA 
AATTCAATTT CAAGCAAAGC AGCTTCTTCC TTTTTCTCAA CCCAGGCCCA TGTGGCATGT 
GAGCACCCAA CTGGATGGAG CAGCATGGAG GAGCGCCATC TGCTGCTCAC CTTGGCAGGG 
CACTGGCTTG CCCAGGAGGA CGTGGTGCCC TTGGATAAGC TGGAGGAGCT GGAGAAGCAG 
ATCTGGCTGT GCCGCATCAC CCAGCACACT CTTGGAAGAA ATCAGGAGGA AACAGAGCCC 
AGATTTTCTC GACAGATCTC AACTAGTGGT GAACTTTCCT TTGATAGTTT AGCCAGTGAG 
TTTTCCTTCT CCAAGTTGGC TGCTCTGAAC ACATCAAAAT ACTTAGAACT TAACAGCCTT 
CCATCCAAAG AGACATGCGA GAATAGATTG GATTGGAAAG AGCAGGAGTC ACTAAACTTT 
TTGATTGGGC GCCTACTGGA TGATGGCTGT GTGCATGAAG CAAGTAGAGT ATGCCGGTAT 
TTTCATTTTT ATAATCCAGA TGTCGCCTTG GTATTGCACT GCAGAGCACT GGCCTCAGGG 
GAAGCTAGTA TGGAGGATCT GCACCCAGAG ATCCATGCTC TCCTACAAAG TGCTGAGCTG 
CTTGAGGAAG AAGCACCCGA CATTCCCCTA AGGAGAGTCC ACAGCACTTC AAGTCTGGAT 
AGTCAGAAGT TTGTGACAGT GCCCTCCAGT AATGAAGTGG TAACTAACCT GGAAGTGCTG 
ACAAGCAAAT GCCTCCATGG GAAGAACTAC TGTCGACAGG TCCTCTGTCT GTATGATCTT 
GCCAAGGAGT TGGGCTGTTC CTACACAGAT GTTGCTGCTC AGGATGGTGA AGCCATGCTC 
CGGAAAATCT TGGCCTCTCA GCAGCCTGAC CGATGCAAAC GAGCCCAGGC CTTCATCAGC 
ACACAGGGCC TTAAGCCAGA TACTGTGGCT GAACTCGTGG CAGAAGAGGT GACACGGGAG 
CTGCTTACTT CATCACAGGG AACAGGACAT AAGCAGATGT TCAACCCAAC AGAGGAAAGC 
CAGACATTTC TTCAGCTGAC CACTCTGTGT CAAGACCGCA CATTGGTAGG CATGAAGTTG 
TTGGATAAGA TTTCCTCCGT TCCCCATGGG GAACTGTCTT GCACCACAGA GCTCCTGATC 
CTGGCCCATC ATTGCTTCAC CCTGACGTGC CACATGGAGG GCATCATCCG AGTCCTACAG 
GCCGCCCACA TGCTCACAGA TAACCACCTG GCCCCCAGTG AGGAGTATGG GCTGGTGAGT 
GGTACCCTGA AAACAGCCCT GCTGGACTAC ATCAAACGCT GCCGTCCTGG AGACAGTGAA 
AAGCACAATA TGATTGCCCT GTGCTTCAGC ATGTGCCGGG AGATTGGCGA GAACCACGAG 
GCAGCTGCCC GCATCCAACT GAAATTGATT GAGTCTCAGC CCTGGGAGGA CAGCCTCAAG 
GATGGGCACC AGCTGAAACA ACTGCTGCTG AAGGCCCTGA CTCTGATGTT GGATGCAGCA 
GAGAGTTATG CCAAGGACTC CTGTGTGCGA CAGGCCCAGC ACTGTCAGCG GCTCACCAAG 
TTGATAACTC TGCAGATTCA CTTTCTGAAC ACTGGCCAGA ACACAATGCT CATCAACTTG 
GGCCGCCACA AGCTGATGGA CTGTATTCTG GCCCTACCTC GGTTCTACCA GGCTTCTATT 
GTGGCTGAGG CCTACGATTT TGTTCCAGAT TGGGCTGAAA TTTTATACCA GCAAGTGATT 
CTTAAAGGAG ACTTTAATTA CTTGGAAGAA TTTAAGCAGC AAAGGTTATT AAAGTCCAGT 
ATATTTGAAG AGATTTCCAA AAAATATAAA CAACATCAGC CTACTGACAT GGTCATGGAA 
AACCTGAAGA AATTACTCAC ATATTGTGAA GATGTTTACC TGTATTACAA GTTGGCATAC 
GAACACAAGT TTTATGAAAT TGTAAATGTG CTTCTGAAGG ACCCTCAGAC AGGTTGCTGT 
CTAAAGGACA TGCTAGCAGG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878123.1
CDS	32..7255
Translation	MAAEEGVASAASAGGSWGTAAMGRVLPMLLVPVPAEAMGQLGSRAQLRTQPEALGSLTAAGSLQVLSLTPGSRGGGRCCLEGPFWHFLWEDSRNSSTPTEKPKLLALGENYELLIYEFNLKDGRCDATILYSCSREALQKLIDDQDISISLLSLRILSFHNNTSLLFINKCVILHIIFPERDAAIRVLNCFTLPLPAQAVDMIIDTQLCRGILFVLSSLGWIYIFDVVDGTYVAHVDLALHKEDMCNEQQQEPAKISSFTSLKVSQDLDVAVIVSSSNSAVALNLNLYFRQHPGHLLCERILEDLPIQGPKGVDEDDPVNSAYNMKLAKFSFQIDRSWKAQLSSLNETIKNSKLEVSCCAPWFQDILHLESPESGNHSTSVQSWAFIPQDIMHGQYNVLQKDHAKTSDPGRSWKIMHISEQEEPIELKCVSVTGFTALFTWEVERMGYTITLWDLETQGMQCFSLGTKCIPVDSSGDQQLCFVLTENGLSLILFGLTQEEFLNRLMIHGSASTVDTLCHLNGWGRCSIPIHALEAGIENRQLDTVNFFLKSKENLFNPSSKSSVSDQFDHLSSHLYLRNVEELIPALDLLCSAIRESYSEPQSKHFSEQLLNLTLSFLNNQIKELFIHTEELDEHLQKGVNILTSYINELRTFMIKFPWKLTDAIDEYDVHENVPKVKESNIWKKLSFEEVIASAILNNKIPEAQTFFRIDSHSAQKLEELIGIGLNLVFDNLKKNNIKEASELLKNMGFDVKGQLLKICFYTTNKNIRDFLVEILKEKNYFSEKEKRTIDFVHQVEKLYLGHFQENMQIQSFPRYWIKEQDFFKHKSVLDSFLKYDCKDEFNKQDHRIVLNWALWWDQLTQESILLPRISPEEYKSYSPEALWRYLTARHDWLNIILWIGEFQTQHSYASLQQNKWPLLTVDVINQNTSCNNYMRNEILDKLARNGVFLASELEDFECFLLRLSRIGGVIQDTLPVQNYKTKEGWDFHSQFILYCLEHSLQHLLYVYLDCYKLSPENCPFLEKKELHEAHPWFEFLVQCRQVASNLTDPKLIFQASLANAQILIPTNQASVSSMLLEGHTLLALATTMYSPGGVSQVVQNEENENCLKKVDPQLLKMALTPYPKLKTALFPQCTPPSVLPSDITIYHLIQSLSPFDPSRLFGWQSANTLAIGDAWSHLPHFSSPDLVNKYAIVERLNFAYYLHNGRPSFAFGTFLVQELIKSKTPKQLIQQVGNEAYVIGLSSFHIPSIGAACVCFLELLGLDSLKLRVDMKVANIILSYKCRNEDAQYSFIRESVAEKLSKLADGEKTTTEELLVLLEEGTWNSIQQQEIKRLSSESSSQWALVVQFCRLHNMKLSISYLRECAKANDWLQFIIHSQLHNYHPAEVKSLIQYFSPVIQDHLRLAFENLPSVPTSKMDSDQVCNKCPQELQGSKQEMTDLFEILLQCSEEPDSWHWLLVEAVKQQAPILSVLASCLQGASAISCLCVWIITSVEDNVATEAMGHIQDSTEDHTWNLEDLSVIWRTLLTRQKSKTLIRGFQLFFKDSPLLLVMEMYELCMFFRNYKEAEAKLLEFQKSLETLNTAATKVHPVIPAMWLEDQVCFLLKLMLQQCKTQYELGKLLQLFVEREHLFSDGPDVKKLCILCQILKDTSIAINHTIITSYSIENLQHECRSILERLQTDGQFALARRVAELAELPVDNLVIKEITQEMQTLKHIEQWSLKQARIDFWKKCHENFKKNSISSKAASSFFSTQAHVACEHPTGWSSMEERHLLLTLAGHWLAQEDVVPLDKLEELEKQIWLCRITQHTLGRNQEETEPRFSRQISTSGELSFDSLASEFSFSKLAALNTSKYLELNSLPSKETCENRLDWKEQESLNFLIGRLLDDGCVHEASRVCRYFHFYNPDVALVLHCRALASGEASMEDLHPEIHALLQSAELLEEEAPDIPLRRVHSTSSLDSQKFVTVPSSNEVVTNLEVLTSKCLHGKNYCRQVLCLYDLAKELGCSYTDVAAQDGEAMLRKILASQQPDRCKRAQAFISTQGLKPDTVAELVAEEVTRELLTSSQGTGHKQMFNPTEESQTFLQLTTLCQDRTLVGMKLLDKISSVPHGELSCTTELLILAHHCFTLTCHMEGIIRVLQAAHMLTDNHLAPSEEYGLVSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAARIQLKLIESQPWEDSLKDGHQLKQLLLKALTLMLDAAESYAKDSCVRQAQHCQRLTKLITLQIHFLNTGQNTMLINLGRHKLMDCILALPRFYQASIVAEAYDFVPDWAEILYQQVILKGDFNYLEEFKQQRLLKSSIFEEISKKYKQHQPTDMVMENLKKLLTYCEDVYLYYKLAYEHKFYEIVNVLLKDPQTGCCLKDMLAG

Target ORF information:

RefSeq Version	XM_017022634.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022634.1

ORF Insert Sequence:

ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG 
CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG 
GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG 
GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA 
AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG 
AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG 
CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC 
AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA 
AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG 
GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC 
TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT 
CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT 
TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA 
GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA 
ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC 
TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC 
CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT 
CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT 
GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG 
AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA 
CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT 
TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG 
CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG 
TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG 
TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC 
AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT 
CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA 
AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG 
GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA 
CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC 
CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG 
AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG 
CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC 
AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA 
ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG 
CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA 
GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC 
TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT 
TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT 
TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA 
CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT 
GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA 
ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT 
GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT 
GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG 
ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA 
GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC 
CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC 
AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT 
CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC 
TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT 
CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT 
GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT 
ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG 
AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA 
ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG 
CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA 
TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA 
CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT 
TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA 
ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA 
GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG 
CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC 
TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA 
CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA 
GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC 
AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT 
TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC 
CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA 
GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC 
GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT 
TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT 
GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC 
AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT 
GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT 
TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC 
CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG 
TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA 
ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT 
CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG 
AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA 
AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT 
ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG 
CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG 
GACAACTTGG TTATTAAAGA GATAACACAG GAAATGCAGA CCCTAAAACA CATTGAACAG 
TGGTCACTAA AACAAGCAAG AATTGACTTC TGGAAAAAAT GCCATGAGAA TTTTAAGAAA 
AATTCAATTT CAAGCAAAGC AGCTTCTTCC TTTTTCTCAA CCCAGGCCCA TGTGGCATGT 
GAGCACCCAA CTGGATGGAG CAGCATGGAG GAGCGCCATC TGCTGCTCAC CTTGGCAGGG 
CACTGGCTTG CCCAGGAGGA CGTGGTGCCC TTGGATAAGC TGGAGGAGCT GGAGAAGCAG 
ATCTGGCTGT GCCGCATCAC CCAGCACACT CTTGGAAGAA ATCAGGAGGA AACAGAGCCC 
AGATTTTCTC GACAGATCTC AACTAGTGGT GAACTTTCCT TTGATAGTTT AGCCAGTGAG 
TTTTCCTTCT CCAAGTTGGC TGCTCTGAAC ACATCAAAAT ACTTAGAACT TAACAGCCTT 
CCATCCAAAG AGACATGCGA GAATAGATTG GATTGGAAAG AGCAGGAGTC ACTAAACTTT 
TTGATTGGGC GCCTACTGGA TGATGGCTGT GTGCATGAAG CAAGTAGAGT ATGCCGGTAT 
TTTCATTTTT ATAATCCAGA TGTCGCCTTG GTATTGCACT GCAGAGCACT GGCCTCAGGG 
GAAGCTAGTA TGGAGGATCT GCACCCAGAG ATCCATGCTC TCCTACAAAG TGCTGAGCTG 
CTTGAGGAAG AAGCACCCGA CATTCCCCTA AGGAGAGTCC ACAGCACTTC AAGTCTGGAT 
AGTCAGAAGT TTGTGACAGT GCCCTCCAGT AATGAAGTGG TAACTAACCT GGAAGTGCTG 
ACAAGCAAAT GCCTCCATGG GAAGAACTAC TGTCGACAGG TCCTCTGTCT GTATGATCTT 
GCCAAGGAGT TGGGCTGTTC CTACACAGAT GTTGCTGCTC AGGATGGTGA AGCCATGCTC 
CGGAAAATCT TGGCCTCTCA GCAGCCTGAC CGATGCAAAC GAGCCCAGGC CTTCATCAGC 
ACACAGGGCC TTAAGCCAGA TACTGTGGCT GAACTCGTGG CAGAAGAGGT GACACGGGAG 
CTGCTTACTT CATCACAGGG AACAGGACAT AAGCAGATGT TCAACCCAAC AGAGGAAAGC 
CAGACATTTC TTCAGCTGAC CACTCTGTGT CAAGACCGCA CATTGGTAGG CATGAAGTTG 
TTGGATAAGA TTTCCTCCGT TCCCCATGGG GAACTGTCTT GCACCACAGA GCTCCTGATC 
CTGGCCCATC ATTGCTTCAC CCTGACGTGC CACATGGAGG GCATCATCCG AGTCCTACAG 
GCCGCCCACA TGCTCACAGA TAACCACCTG GCCCCCAGTG AGGAGTATGG GCTGGTGAGT 
GGTACCCTGA AAACAGCCCT GCTGGACTAC ATCAAACGCT GCCGTCCTGG AGACAGTGAA 
AAGCACAATA TGATTGCCCT GTGCTTCAGC ATGTGCCGGG AGATTGGCGA GAACCACGAG 
GCAGCTGCCC GCATCCAACT GAAATTGATT GAGTCTCAGC CCTGGGAGGA CAGCCTCAAG 
GATGGGCACC AGCTGAAACA ACTGCTGCTG AAGGCCCTGA CTCTGATGTT GGATGCAGCA 
GAGAGTTATG CCAAGGACTC CTGTGTGCGA CAGGCCCAGC ACTGTCAGCG GCTCACCAAG 
TTGATAACTC TGCAGATTCA CTTTCTGAAC ACTGGCCAGA ACACAATGCT CATCAACTTG 
GGCCGCCACA AGCTGATGGA CTGTATTCTG GCCCTACCTC GGTTCTACCA GGCTTCTATT 
GTGGCTGAGG CCTACGATTT TGTTCCAGAT TGGGCTGAAA TTTTATACCA GCAAGTGATT 
CTTAAAGGAG ACTTTAATTA CTTGGAAGAA TTTAAGCAGC AAAGGTTATT AAAGTCCAGT 
ATATTTGAAG AGATTTCCAA AAAATATAAA CAACATCAGC CTACTGACAT GGTCATGGAA 
AACCTGAAGA AATTACTCAC ATATTGTGAA GATGTTTACC TGTATTACAA GTTGGCATAC 
GAACACAAGT TTTATGAAAT TGTAAATGTG CTTCTGAAGG ACCCTCAGAC AGGTTGCTGT 
CTAAAGGACA TGCTAGCAGG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( NM_001160227.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001153699.1 Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant 2, mRNA.

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CloneID	OHu16493
Clone ID Related Accession (Same CDS sequence)	NM_001160227.1 , NM_001160227.2
Accession Version	NM_001160227.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	spatacsin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA926289.1, BC150640.1 and BC094704.1. Transcript Variant: This variant (2) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150640.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG 
CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG 
GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG 
GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA 
AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG 
AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG 
CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC 
AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA 
AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG 
GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC 
TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT 
CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT 
TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA 
GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA 
ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC 
TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC 
CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT 
CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT 
GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG 
AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA 
CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT 
TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG 
CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG 
TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG 
TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC 
AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT 
CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA 
AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG 
GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA 
CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC 
CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG 
AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG 
CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC 
AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA 
ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG 
CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA 
GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC 
TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT 
TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT 
TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA 
CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT 
GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA 
ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT 
GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT 
GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG 
ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA 
GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC 
CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC 
AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT 
CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC 
TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT 
CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT 
GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT 
ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG 
AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA 
ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG 
CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA 
TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA 
CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT 
TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA 
ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA 
GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG 
CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC 
TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA 
CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA 
GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC 
AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT 
TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC 
CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA 
GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC 
GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT 
TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT 
GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC 
AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT 
GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT 
TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC 
CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG 
TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA 
ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT 
CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG 
AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA 
AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT 
ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG 
CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG 
GACAACTTGG TTATTAAAGA GATAACACAG GAAATGCAGA CCCTAAAACA CATTGAACAG 
TGGTCACTAA AACAAGCAAG AATTGACTTC TGGAAAAAAT GCCATGAGAA TTTTAAGAAA 
AATTCAATTT CAAGCAAAGC AGCTTCTTCC TTTTTCTCAA CCCAGGCCCA TGTGGCATGT 
GAGCACCCAA CTGGATGGAG CAGCATGGAG GAGCGCCATC TGCTGCTCAC CTTGGCAGGG 
CACTGGCTTG CCCAGGAGGA CGTGGTGCCC TTGGATAAGC TGGAGGAGCT GGAGAAGCAG 
ATCTGGCTGT GCCGCATCAC CCAGCACACT CTTGGAAGAA ATCAGGAGGA AACAGAGCCC 
AGATTTTCTC GACAGATCTC AACTAGTGGT GAACTTTCCT TTGATAGTTT AGCCAGTGAG 
TTTTCCTTCT CCAAGTTGGC TGCTCTGAAC ACATCAAAAT ACTTAGAACT TAACAGCCTT 
CCATCCAAAG AGACATGCGA GAATAGATTG GATTGGAAAG AGCAGGAGTC ACTAAACTTT 
TTGATTGGGC GCCTACTGGA TGATGGCTGT GTGCATGAAG CAAGTAGAGT ATGCCGGTAT 
TTTCATTTTT ATAATCCAGA TGTCGCCTTG GTATTGCACT GCAGAGCACT GGCCTCAGGG 
GAAGCTAGTA TGGAGGATCT GCACCCAGAG ATCCATGCTC TCCTACAAAG TGCTGAGCTG 
CTTGAGGAAG AAGCACCCGA CATTCCCCTA AGGAGAGTCC ACAGCAGACA TAAGCAGATG 
TTCAACCCAA CAGAGGAAAG CCAGACATTT CTTCAGCTGA CCACTCTGTG TCAAGACCGC 
ACATTGGTAG GCATGAAGTT GTTGGATAAG ATTTCCTCCG TTCCCCATGG GGAACTGTCT 
TGCACCACAG AGCTCCTGAT CCTGGCCCAT CATTGCTTCA CCCTGACGTG CCACATGGAG 
GGCATCATCC GAGTCCTACA GGCCGCCCAC ATGCTCACAG ATAACCACCT GGCCCCCAGT 
GAGGAGTATG GGCTGGTGGT ACGGCTCCTC ACTGGCATTG GAAGGTACAA CGAGATGACA 
TACATATTTG ATTTGCTGCA TAAAAAGCAC TACTTTGAAG TGCTAATGAG GAAGAAGTTG 
GATCCGAGTG GTACCCTGAA AACAGCCCTG CTGGACTACA TCAAACGCTG CCGTCCTGGA 
GACAGTGAAA AGCACAATAT GATTGCCCTG TGCTTCAGCA TGTGCCGGGA GATTGGCGAG 
AACCACGAGG CAGCTGCCCG CATCCAACTG AAATTGATTG AGTCTCAGCC CTGGGAGGAC 
AGCCTCAAGG ATGGGCACCA GCTGAAACAA CTGCTGCTGA AGGCCCTGAC TCTGATGTTG 
GATGCAGCAG AGAGTTATGC CAAGGACTCC TGTGTGCGAC AGGCCCAGCA CTGTCAGCGG 
CTCACCAAGT TGATAACTCT GCAGATTCAC TTTCTGAACA CTGGCCAGAA CACAATGCTC 
ATCAACTTGG GCCGCCACAA GCTGATGGAC TGTATTCTGG CCCTACCTCG GTTCTACCAG 
GCTTCTATTG TGGCTGAGGC CTACGATTTT GTTCCAGATT GGGCTGAAAT TTTATACCAG 
CAAGTGATTC TTAAAGGAGA CTTTAATTAC TTGGAAGAAT TTAAGCAGCA AAGGTTATTA 
AAGTCCAGTA TATTTGAAGA GATTTCCAAA AAATATAAAC AACATCAGCC TACTGACATG 
GTCATGGAAA ACCTGAAGAA ATTACTCACA TATTGTGAAG ATGTTTACCT GTATTACAAG 
TTGGCATACG AACACAAGTT TTATGAAATT GTAAATGTGC TTCTGAAGGA CCCTCAGACA 
GGTTGCTGTC TAAAGGACAT GCTAGCAGGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153699.1
CDS	32..7024
Translation	MAAEEGVASAASAGGSWGTAAMGRVLPMLLVPVPAEAMGQLGSRAQLRTQPEALGSLTAAGSLQVLSLTPGSRGGGRCCLEGPFWHFLWEDSRNSSTPTEKPKLLALGENYELLIYEFNLKDGRCDATILYSCSREALQKLIDDQDISISLLSLRILSFHNNTSLLFINKCVILHIIFPERDAAIRVLNCFTLPLPAQAVDMIIDTQLCRGILFVLSSLGWIYIFDVVDGTYVAHVDLALHKEDMCNEQQQEPAKISSFTSLKVSQDLDVAVIVSSSNSAVALNLNLYFRQHPGHLLCERILEDLPIQGPKGVDEDDPVNSAYNMKLAKFSFQIDRSWKAQLSSLNETIKNSKLEVSCCAPWFQDILHLESPESGNHSTSVQSWAFIPQDIMHGQYNVLQKDHAKTSDPGRSWKIMHISEQEEPIELKCVSVTGFTALFTWEVERMGYTITLWDLETQGMQCFSLGTKCIPVDSSGDQQLCFVLTENGLSLILFGLTQEEFLNRLMIHGSASTVDTLCHLNGWGRCSIPIHALEAGIENRQLDTVNFFLKSKENLFNPSSKSSVSDQFDHLSSHLYLRNVEELIPALDLLCSAIRESYSEPQSKHFSEQLLNLTLSFLNNQIKELFIHTEELDEHLQKGVNILTSYINELRTFMIKFPWKLTDAIDEYDVHENVPKVKESNIWKKLSFEEVIASAILNNKIPEAQTFFRIDSHSAQKLEELIGIGLNLVFDNLKKNNIKEASELLKNMGFDVKGQLLKICFYTTNKNIRDFLVEILKEKNYFSEKEKRTIDFVHQVEKLYLGHFQENMQIQSFPRYWIKEQDFFKHKSVLDSFLKYDCKDEFNKQDHRIVLNWALWWDQLTQESILLPRISPEEYKSYSPEALWRYLTARHDWLNIILWIGEFQTQHSYASLQQNKWPLLTVDVINQNTSCNNYMRNEILDKLARNGVFLASELEDFECFLLRLSRIGGVIQDTLPVQNYKTKEGWDFHSQFILYCLEHSLQHLLYVYLDCYKLSPENCPFLEKKELHEAHPWFEFLVQCRQVASNLTDPKLIFQASLANAQILIPTNQASVSSMLLEGHTLLALATTMYSPGGVSQVVQNEENENCLKKVDPQLLKMALTPYPKLKTALFPQCTPPSVLPSDITIYHLIQSLSPFDPSRLFGWQSANTLAIGDAWSHLPHFSSPDLVNKYAIVERLNFAYYLHNGRPSFAFGTFLVQELIKSKTPKQLIQQVGNEAYVIGLSSFHIPSIGAACVCFLELLGLDSLKLRVDMKVANIILSYKCRNEDAQYSFIRESVAEKLSKLADGEKTTTEELLVLLEEGTWNSIQQQEIKRLSSESSSQWALVVQFCRLHNMKLSISYLRECAKANDWLQFIIHSQLHNYHPAEVKSLIQYFSPVIQDHLRLAFENLPSVPTSKMDSDQVCNKCPQELQGSKQEMTDLFEILLQCSEEPDSWHWLLVEAVKQQAPILSVLASCLQGASAISCLCVWIITSVEDNVATEAMGHIQDSTEDHTWNLEDLSVIWRTLLTRQKSKTLIRGFQLFFKDSPLLLVMEMYELCMFFRNYKEAEAKLLEFQKSLETLNTAATKVHPVIPAMWLEDQVCFLLKLMLQQCKTQYELGKLLQLFVEREHLFSDGPDVKKLCILCQILKDTSIAINHTIITSYSIENLQHECRSILERLQTDGQFALARRVAELAELPVDNLVIKEITQEMQTLKHIEQWSLKQARIDFWKKCHENFKKNSISSKAASSFFSTQAHVACEHPTGWSSMEERHLLLTLAGHWLAQEDVVPLDKLEELEKQIWLCRITQHTLGRNQEETEPRFSRQISTSGELSFDSLASEFSFSKLAALNTSKYLELNSLPSKETCENRLDWKEQESLNFLIGRLLDDGCVHEASRVCRYFHFYNPDVALVLHCRALASGEASMEDLHPEIHALLQSAELLEEEAPDIPLRRVHSRHKQMFNPTEESQTFLQLTTLCQDRTLVGMKLLDKISSVPHGELSCTTELLILAHHCFTLTCHMEGIIRVLQAAHMLTDNHLAPSEEYGLVVRLLTGIGRYNEMTYIFDLLHKKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAARIQLKLIESQPWEDSLKDGHQLKQLLLKALTLMLDAAESYAKDSCVRQAQHCQRLTKLITLQIHFLNTGQNTMLINLGRHKLMDCILALPRFYQASIVAEAYDFVPDWAEILYQQVILKGDFNYLEEFKQQRLLKSSIFEEISKKYKQHQPTDMVMENLKKLLTYCEDVYLYYKLAYEHKFYEIVNVLLKDPQTGCCLKDMLAG

Target ORF information:

RefSeq Version	NM_001160227.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160227.1

ORF Insert Sequence:

ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG 
CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG 
GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG 
GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA 
AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG 
AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG 
CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC 
AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA 
AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG 
GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC 
TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT 
CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT 
TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA 
GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA 
ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC 
TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC 
CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT 
CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT 
GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG 
AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA 
CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT 
TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG 
CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG 
TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG 
TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC 
AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT 
CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA 
AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG 
GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA 
CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC 
CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG 
AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG 
CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC 
AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA 
ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG 
CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA 
GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC 
TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT 
TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT 
TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA 
CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT 
GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA 
ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT 
GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT 
GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG 
ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA 
GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC 
CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC 
AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT 
CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC 
TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT 
CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT 
GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT 
ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG 
AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA 
ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG 
CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA 
TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA 
CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT 
TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA 
ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA 
GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG 
CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC 
TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA 
CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA 
GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC 
AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT 
TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC 
CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA 
GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC 
GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT 
TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT 
GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC 
AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT 
GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT 
TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC 
CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG 
TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA 
ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT 
CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG 
AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA 
AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT 
ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG 
CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG 
GACAACTTGG TTATTAAAGA GATAACACAG GAAATGCAGA CCCTAAAACA CATTGAACAG 
TGGTCACTAA AACAAGCAAG AATTGACTTC TGGAAAAAAT GCCATGAGAA TTTTAAGAAA 
AATTCAATTT CAAGCAAAGC AGCTTCTTCC TTTTTCTCAA CCCAGGCCCA TGTGGCATGT 
GAGCACCCAA CTGGATGGAG CAGCATGGAG GAGCGCCATC TGCTGCTCAC CTTGGCAGGG 
CACTGGCTTG CCCAGGAGGA CGTGGTGCCC TTGGATAAGC TGGAGGAGCT GGAGAAGCAG 
ATCTGGCTGT GCCGCATCAC CCAGCACACT CTTGGAAGAA ATCAGGAGGA AACAGAGCCC 
AGATTTTCTC GACAGATCTC AACTAGTGGT GAACTTTCCT TTGATAGTTT AGCCAGTGAG 
TTTTCCTTCT CCAAGTTGGC TGCTCTGAAC ACATCAAAAT ACTTAGAACT TAACAGCCTT 
CCATCCAAAG AGACATGCGA GAATAGATTG GATTGGAAAG AGCAGGAGTC ACTAAACTTT 
TTGATTGGGC GCCTACTGGA TGATGGCTGT GTGCATGAAG CAAGTAGAGT ATGCCGGTAT 
TTTCATTTTT ATAATCCAGA TGTCGCCTTG GTATTGCACT GCAGAGCACT GGCCTCAGGG 
GAAGCTAGTA TGGAGGATCT GCACCCAGAG ATCCATGCTC TCCTACAAAG TGCTGAGCTG 
CTTGAGGAAG AAGCACCCGA CATTCCCCTA AGGAGAGTCC ACAGCAGACA TAAGCAGATG 
TTCAACCCAA CAGAGGAAAG CCAGACATTT CTTCAGCTGA CCACTCTGTG TCAAGACCGC 
ACATTGGTAG GCATGAAGTT GTTGGATAAG ATTTCCTCCG TTCCCCATGG GGAACTGTCT 
TGCACCACAG AGCTCCTGAT CCTGGCCCAT CATTGCTTCA CCCTGACGTG CCACATGGAG 
GGCATCATCC GAGTCCTACA GGCCGCCCAC ATGCTCACAG ATAACCACCT GGCCCCCAGT 
GAGGAGTATG GGCTGGTGGT ACGGCTCCTC ACTGGCATTG GAAGGTACAA CGAGATGACA 
TACATATTTG ATTTGCTGCA TAAAAAGCAC TACTTTGAAG TGCTAATGAG GAAGAAGTTG 
GATCCGAGTG GTACCCTGAA AACAGCCCTG CTGGACTACA TCAAACGCTG CCGTCCTGGA 
GACAGTGAAA AGCACAATAT GATTGCCCTG TGCTTCAGCA TGTGCCGGGA GATTGGCGAG 
AACCACGAGG CAGCTGCCCG CATCCAACTG AAATTGATTG AGTCTCAGCC CTGGGAGGAC 
AGCCTCAAGG ATGGGCACCA GCTGAAACAA CTGCTGCTGA AGGCCCTGAC TCTGATGTTG 
GATGCAGCAG AGAGTTATGC CAAGGACTCC TGTGTGCGAC AGGCCCAGCA CTGTCAGCGG 
CTCACCAAGT TGATAACTCT GCAGATTCAC TTTCTGAACA CTGGCCAGAA CACAATGCTC 
ATCAACTTGG GCCGCCACAA GCTGATGGAC TGTATTCTGG CCCTACCTCG GTTCTACCAG 
GCTTCTATTG TGGCTGAGGC CTACGATTTT GTTCCAGATT GGGCTGAAAT TTTATACCAG 
CAAGTGATTC TTAAAGGAGA CTTTAATTAC TTGGAAGAAT TTAAGCAGCA AAGGTTATTA 
AAGTCCAGTA TATTTGAAGA GATTTCCAAA AAATATAAAC AACATCAGCC TACTGACATG 
GTCATGGAAA ACCTGAAGAA ATTACTCACA TATTGTGAAG ATGTTTACCT GTATTACAAG 
TTGGCATACG AACACAAGTT TTATGAAATT GTAAATGTGC TTCTGAAGGA CCCTCAGACA 
GGTTGCTGTC TAAAGGACAT GCTAGCAGGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( NM_001160227.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001153699.1 Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant 2, mRNA.

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CloneID	OHu16493
Clone ID Related Accession (Same CDS sequence)	NM_001160227.1 , NM_001160227.2
Accession Version	NM_001160227.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	spatacsin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC150640.1 and BC094704.1. On May 31, 2019 this sequence version replaced NM_001160227.1. Transcript Variant: This variant (2) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150640.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG 
CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG 
GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG 
GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA 
AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG 
AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG 
CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC 
AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA 
AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG 
GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC 
TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT 
CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT 
TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA 
GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA 
ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC 
TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC 
CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT 
CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT 
GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG 
AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA 
CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT 
TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG 
CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG 
TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG 
TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC 
AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT 
CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA 
AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG 
GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA 
CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC 
CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG 
AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG 
CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC 
AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA 
ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG 
CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA 
GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC 
TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT 
TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT 
TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA 
CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT 
GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA 
ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT 
GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT 
GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG 
ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA 
GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC 
CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC 
AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT 
CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC 
TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT 
CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT 
GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT 
ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG 
AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA 
ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG 
CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA 
TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA 
CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT 
TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA 
ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA 
GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG 
CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC 
TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA 
CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA 
GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC 
AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT 
TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC 
CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA 
GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC 
GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT 
TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT 
GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC 
AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT 
GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT 
TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC 
CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG 
TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA 
ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT 
CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG 
AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA 
AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT 
ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG 
CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG 
GACAACTTGG TTATTAAAGA GATAACACAG GAAATGCAGA CCCTAAAACA CATTGAACAG 
TGGTCACTAA AACAAGCAAG AATTGACTTC TGGAAAAAAT GCCATGAGAA TTTTAAGAAA 
AATTCAATTT CAAGCAAAGC AGCTTCTTCC TTTTTCTCAA CCCAGGCCCA TGTGGCATGT 
GAGCACCCAA CTGGATGGAG CAGCATGGAG GAGCGCCATC TGCTGCTCAC CTTGGCAGGG 
CACTGGCTTG CCCAGGAGGA CGTGGTGCCC TTGGATAAGC TGGAGGAGCT GGAGAAGCAG 
ATCTGGCTGT GCCGCATCAC CCAGCACACT CTTGGAAGAA ATCAGGAGGA AACAGAGCCC 
AGATTTTCTC GACAGATCTC AACTAGTGGT GAACTTTCCT TTGATAGTTT AGCCAGTGAG 
TTTTCCTTCT CCAAGTTGGC TGCTCTGAAC ACATCAAAAT ACTTAGAACT TAACAGCCTT 
CCATCCAAAG AGACATGCGA GAATAGATTG GATTGGAAAG AGCAGGAGTC ACTAAACTTT 
TTGATTGGGC GCCTACTGGA TGATGGCTGT GTGCATGAAG CAAGTAGAGT ATGCCGGTAT 
TTTCATTTTT ATAATCCAGA TGTCGCCTTG GTATTGCACT GCAGAGCACT GGCCTCAGGG 
GAAGCTAGTA TGGAGGATCT GCACCCAGAG ATCCATGCTC TCCTACAAAG TGCTGAGCTG 
CTTGAGGAAG AAGCACCCGA CATTCCCCTA AGGAGAGTCC ACAGCAGACA TAAGCAGATG 
TTCAACCCAA CAGAGGAAAG CCAGACATTT CTTCAGCTGA CCACTCTGTG TCAAGACCGC 
ACATTGGTAG GCATGAAGTT GTTGGATAAG ATTTCCTCCG TTCCCCATGG GGAACTGTCT 
TGCACCACAG AGCTCCTGAT CCTGGCCCAT CATTGCTTCA CCCTGACGTG CCACATGGAG 
GGCATCATCC GAGTCCTACA GGCCGCCCAC ATGCTCACAG ATAACCACCT GGCCCCCAGT 
GAGGAGTATG GGCTGGTGGT ACGGCTCCTC ACTGGCATTG GAAGGTACAA CGAGATGACA 
TACATATTTG ATTTGCTGCA TAAAAAGCAC TACTTTGAAG TGCTAATGAG GAAGAAGTTG 
GATCCGAGTG GTACCCTGAA AACAGCCCTG CTGGACTACA TCAAACGCTG CCGTCCTGGA 
GACAGTGAAA AGCACAATAT GATTGCCCTG TGCTTCAGCA TGTGCCGGGA GATTGGCGAG 
AACCACGAGG CAGCTGCCCG CATCCAACTG AAATTGATTG AGTCTCAGCC CTGGGAGGAC 
AGCCTCAAGG ATGGGCACCA GCTGAAACAA CTGCTGCTGA AGGCCCTGAC TCTGATGTTG 
GATGCAGCAG AGAGTTATGC CAAGGACTCC TGTGTGCGAC AGGCCCAGCA CTGTCAGCGG 
CTCACCAAGT TGATAACTCT GCAGATTCAC TTTCTGAACA CTGGCCAGAA CACAATGCTC 
ATCAACTTGG GCCGCCACAA GCTGATGGAC TGTATTCTGG CCCTACCTCG GTTCTACCAG 
GCTTCTATTG TGGCTGAGGC CTACGATTTT GTTCCAGATT GGGCTGAAAT TTTATACCAG 
CAAGTGATTC TTAAAGGAGA CTTTAATTAC TTGGAAGAAT TTAAGCAGCA AAGGTTATTA 
AAGTCCAGTA TATTTGAAGA GATTTCCAAA AAATATAAAC AACATCAGCC TACTGACATG 
GTCATGGAAA ACCTGAAGAA ATTACTCACA TATTGTGAAG ATGTTTACCT GTATTACAAG 
TTGGCATACG AACACAAGTT TTATGAAATT GTAAATGTGC TTCTGAAGGA CCCTCAGACA 
GGTTGCTGTC TAAAGGACAT GCTAGCAGGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153699.1
CDS	16..7008
Translation	MAAEEGVASAASAGGSWGTAAMGRVLPMLLVPVPAEAMGQLGSRAQLRTQPEALGSLTAAGSLQVLSLTPGSRGGGRCCLEGPFWHFLWEDSRNSSTPTEKPKLLALGENYELLIYEFNLKDGRCDATILYSCSREALQKLIDDQDISISLLSLRILSFHNNTSLLFINKCVILHIIFPERDAAIRVLNCFTLPLPAQAVDMIIDTQLCRGILFVLSSLGWIYIFDVVDGTYVAHVDLALHKEDMCNEQQQEPAKISSFTSLKVSQDLDVAVIVSSSNSAVALNLNLYFRQHPGHLLCERILEDLPIQGPKGVDEDDPVNSAYNMKLAKFSFQIDRSWKAQLSSLNETIKNSKLEVSCCAPWFQDILHLESPESGNHSTSVQSWAFIPQDIMHGQYNVLQKDHAKTSDPGRSWKIMHISEQEEPIELKCVSVTGFTALFTWEVERMGYTITLWDLETQGMQCFSLGTKCIPVDSSGDQQLCFVLTENGLSLILFGLTQEEFLNRLMIHGSASTVDTLCHLNGWGRCSIPIHALEAGIENRQLDTVNFFLKSKENLFNPSSKSSVSDQFDHLSSHLYLRNVEELIPALDLLCSAIRESYSEPQSKHFSEQLLNLTLSFLNNQIKELFIHTEELDEHLQKGVNILTSYINELRTFMIKFPWKLTDAIDEYDVHENVPKVKESNIWKKLSFEEVIASAILNNKIPEAQTFFRIDSHSAQKLEELIGIGLNLVFDNLKKNNIKEASELLKNMGFDVKGQLLKICFYTTNKNIRDFLVEILKEKNYFSEKEKRTIDFVHQVEKLYLGHFQENMQIQSFPRYWIKEQDFFKHKSVLDSFLKYDCKDEFNKQDHRIVLNWALWWDQLTQESILLPRISPEEYKSYSPEALWRYLTARHDWLNIILWIGEFQTQHSYASLQQNKWPLLTVDVINQNTSCNNYMRNEILDKLARNGVFLASELEDFECFLLRLSRIGGVIQDTLPVQNYKTKEGWDFHSQFILYCLEHSLQHLLYVYLDCYKLSPENCPFLEKKELHEAHPWFEFLVQCRQVASNLTDPKLIFQASLANAQILIPTNQASVSSMLLEGHTLLALATTMYSPGGVSQVVQNEENENCLKKVDPQLLKMALTPYPKLKTALFPQCTPPSVLPSDITIYHLIQSLSPFDPSRLFGWQSANTLAIGDAWSHLPHFSSPDLVNKYAIVERLNFAYYLHNGRPSFAFGTFLVQELIKSKTPKQLIQQVGNEAYVIGLSSFHIPSIGAACVCFLELLGLDSLKLRVDMKVANIILSYKCRNEDAQYSFIRESVAEKLSKLADGEKTTTEELLVLLEEGTWNSIQQQEIKRLSSESSSQWALVVQFCRLHNMKLSISYLRECAKANDWLQFIIHSQLHNYHPAEVKSLIQYFSPVIQDHLRLAFENLPSVPTSKMDSDQVCNKCPQELQGSKQEMTDLFEILLQCSEEPDSWHWLLVEAVKQQAPILSVLASCLQGASAISCLCVWIITSVEDNVATEAMGHIQDSTEDHTWNLEDLSVIWRTLLTRQKSKTLIRGFQLFFKDSPLLLVMEMYELCMFFRNYKEAEAKLLEFQKSLETLNTAATKVHPVIPAMWLEDQVCFLLKLMLQQCKTQYELGKLLQLFVEREHLFSDGPDVKKLCILCQILKDTSIAINHTIITSYSIENLQHECRSILERLQTDGQFALARRVAELAELPVDNLVIKEITQEMQTLKHIEQWSLKQARIDFWKKCHENFKKNSISSKAASSFFSTQAHVACEHPTGWSSMEERHLLLTLAGHWLAQEDVVPLDKLEELEKQIWLCRITQHTLGRNQEETEPRFSRQISTSGELSFDSLASEFSFSKLAALNTSKYLELNSLPSKETCENRLDWKEQESLNFLIGRLLDDGCVHEASRVCRYFHFYNPDVALVLHCRALASGEASMEDLHPEIHALLQSAELLEEEAPDIPLRRVHSRHKQMFNPTEESQTFLQLTTLCQDRTLVGMKLLDKISSVPHGELSCTTELLILAHHCFTLTCHMEGIIRVLQAAHMLTDNHLAPSEEYGLVVRLLTGIGRYNEMTYIFDLLHKKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAARIQLKLIESQPWEDSLKDGHQLKQLLLKALTLMLDAAESYAKDSCVRQAQHCQRLTKLITLQIHFLNTGQNTMLINLGRHKLMDCILALPRFYQASIVAEAYDFVPDWAEILYQQVILKGDFNYLEEFKQQRLLKSSIFEEISKKYKQHQPTDMVMENLKKLLTYCEDVYLYYKLAYEHKFYEIVNVLLKDPQTGCCLKDMLAG

Target ORF information:

RefSeq Version	NM_001160227.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160227.2

ORF Insert Sequence:

ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG 
CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG 
GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG 
GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA 
AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG 
AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG 
CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC 
AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA 
AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG 
GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC 
TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT 
CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT 
TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA 
GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA 
ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC 
TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC 
CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT 
CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT 
GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG 
AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA 
CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT 
TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG 
CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG 
TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG 
TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC 
AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT 
CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA 
AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG 
GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA 
CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC 
CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG 
AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG 
CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC 
AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA 
ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG 
CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA 
GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC 
TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT 
TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT 
TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA 
CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT 
GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA 
ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT 
GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT 
GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG 
ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA 
GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC 
CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC 
AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT 
CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC 
TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT 
CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT 
GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT 
ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG 
AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA 
ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG 
CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA 
TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA 
CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT 
TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA 
ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA 
GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG 
CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC 
TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA 
CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA 
GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC 
AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT 
TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC 
CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA 
GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC 
GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT 
TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT 
GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC 
AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT 
GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT 
TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC 
CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG 
TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA 
ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT 
CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG 
AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA 
AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT 
ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG 
CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG 
GACAACTTGG TTATTAAAGA GATAACACAG GAAATGCAGA CCCTAAAACA CATTGAACAG 
TGGTCACTAA AACAAGCAAG AATTGACTTC TGGAAAAAAT GCCATGAGAA TTTTAAGAAA 
AATTCAATTT CAAGCAAAGC AGCTTCTTCC TTTTTCTCAA CCCAGGCCCA TGTGGCATGT 
GAGCACCCAA CTGGATGGAG CAGCATGGAG GAGCGCCATC TGCTGCTCAC CTTGGCAGGG 
CACTGGCTTG CCCAGGAGGA CGTGGTGCCC TTGGATAAGC TGGAGGAGCT GGAGAAGCAG 
ATCTGGCTGT GCCGCATCAC CCAGCACACT CTTGGAAGAA ATCAGGAGGA AACAGAGCCC 
AGATTTTCTC GACAGATCTC AACTAGTGGT GAACTTTCCT TTGATAGTTT AGCCAGTGAG 
TTTTCCTTCT CCAAGTTGGC TGCTCTGAAC ACATCAAAAT ACTTAGAACT TAACAGCCTT 
CCATCCAAAG AGACATGCGA GAATAGATTG GATTGGAAAG AGCAGGAGTC ACTAAACTTT 
TTGATTGGGC GCCTACTGGA TGATGGCTGT GTGCATGAAG CAAGTAGAGT ATGCCGGTAT 
TTTCATTTTT ATAATCCAGA TGTCGCCTTG GTATTGCACT GCAGAGCACT GGCCTCAGGG 
GAAGCTAGTA TGGAGGATCT GCACCCAGAG ATCCATGCTC TCCTACAAAG TGCTGAGCTG 
CTTGAGGAAG AAGCACCCGA CATTCCCCTA AGGAGAGTCC ACAGCAGACA TAAGCAGATG 
TTCAACCCAA CAGAGGAAAG CCAGACATTT CTTCAGCTGA CCACTCTGTG TCAAGACCGC 
ACATTGGTAG GCATGAAGTT GTTGGATAAG ATTTCCTCCG TTCCCCATGG GGAACTGTCT 
TGCACCACAG AGCTCCTGAT CCTGGCCCAT CATTGCTTCA CCCTGACGTG CCACATGGAG 
GGCATCATCC GAGTCCTACA GGCCGCCCAC ATGCTCACAG ATAACCACCT GGCCCCCAGT 
GAGGAGTATG GGCTGGTGGT ACGGCTCCTC ACTGGCATTG GAAGGTACAA CGAGATGACA 
TACATATTTG ATTTGCTGCA TAAAAAGCAC TACTTTGAAG TGCTAATGAG GAAGAAGTTG 
GATCCGAGTG GTACCCTGAA AACAGCCCTG CTGGACTACA TCAAACGCTG CCGTCCTGGA 
GACAGTGAAA AGCACAATAT GATTGCCCTG TGCTTCAGCA TGTGCCGGGA GATTGGCGAG 
AACCACGAGG CAGCTGCCCG CATCCAACTG AAATTGATTG AGTCTCAGCC CTGGGAGGAC 
AGCCTCAAGG ATGGGCACCA GCTGAAACAA CTGCTGCTGA AGGCCCTGAC TCTGATGTTG 
GATGCAGCAG AGAGTTATGC CAAGGACTCC TGTGTGCGAC AGGCCCAGCA CTGTCAGCGG 
CTCACCAAGT TGATAACTCT GCAGATTCAC TTTCTGAACA CTGGCCAGAA CACAATGCTC 
ATCAACTTGG GCCGCCACAA GCTGATGGAC TGTATTCTGG CCCTACCTCG GTTCTACCAG 
GCTTCTATTG TGGCTGAGGC CTACGATTTT GTTCCAGATT GGGCTGAAAT TTTATACCAG 
CAAGTGATTC TTAAAGGAGA CTTTAATTAC TTGGAAGAAT TTAAGCAGCA AAGGTTATTA 
AAGTCCAGTA TATTTGAAGA GATTTCCAAA AAATATAAAC AACATCAGCC TACTGACATG 
GTCATGGAAA ACCTGAAGAA ATTACTCACA TATTGTGAAG ATGTTTACCT GTATTACAAG 
TTGGCATACG AACACAAGTT TTATGAAATT GTAAATGTGC TTCTGAAGGA CCCTCAGACA 
GGTTGCTGTC TAAAGGACAT GCTAGCAGGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( NM_025137.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_079413.3 Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant 1, mRNA.

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CloneID	OHu19157
Clone ID Related Accession (Same CDS sequence)	NM_025137.4 , NM_025137.3
Accession Version	NM_025137.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	spatacsin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009996.7, AB058743.2 and BC094704.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_025137.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC153879.1, AB058743.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000261866.12/ ENSP00000261866.7 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG 
CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG 
GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG 
GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA 
AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG 
AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG 
CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC 
AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA 
AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG 
GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC 
TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT 
CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT 
TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA 
GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA 
ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC 
TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC 
CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT 
CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT 
GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG 
AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA 
CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT 
TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG 
CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG 
TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG 
TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC 
AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT 
CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA 
AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG 
GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA 
CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC 
CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG 
AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG 
CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC 
AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA 
ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG 
CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA 
GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC 
TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT 
TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT 
TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA 
CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT 
GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA 
ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT 
GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT 
GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG 
ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA 
GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC 
CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC 
AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT 
CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC 
TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT 
CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT 
GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT 
ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG 
AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA 
ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG 
CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA 
TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA 
CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT 
TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA 
ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA 
GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG 
CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC 
TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA 
CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA 
GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC 
AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT 
TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC 
CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA 
GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC 
GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT 
TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT 
GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC 
AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT 
GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT 
TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC 
CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG 
TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA 
ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT 
CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG 
AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA 
AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT 
ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG 
CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG 
GACAACTTGG TTATTAAAGA GATAACACAG GAAATGCAGA CCCTAAAACA CATTGAACAG 
TGGTCACTAA AACAAGCAAG AATTGACTTC TGGAAAAAAT GCCATGAGAA TTTTAAGAAA 
AATTCAATTT CAAGCAAAGC AGCTTCTTCC TTTTTCTCAA CCCAGGCCCA TGTGGCATGT 
GAGCACCCAA CTGGATGGAG CAGCATGGAG GAGCGCCATC TGCTGCTCAC CTTGGCAGGG 
CACTGGCTTG CCCAGGAGGA CGTGGTGCCC TTGGATAAGC TGGAGGAGCT GGAGAAGCAG 
ATCTGGCTGT GCCGCATCAC CCAGCACACT CTTGGAAGAA ATCAGGAGGA AACAGAGCCC 
AGATTTTCTC GACAGATCTC AACTAGTGGT GAACTTTCCT TTGATAGTTT AGCCAGTGAG 
TTTTCCTTCT CCAAGTTGGC TGCTCTGAAC ACATCAAAAT ACTTAGAACT TAACAGCCTT 
CCATCCAAAG AGACATGCGA GAATAGATTG GATTGGAAAG AGCAGGAGTC ACTAAACTTT 
TTGATTGGGC GCCTACTGGA TGATGGCTGT GTGCATGAAG CAAGTAGAGT ATGCCGGTAT 
TTTCATTTTT ATAATCCAGA TGTCGCCTTG GTATTGCACT GCAGAGCACT GGCCTCAGGG 
GAAGCTAGTA TGGAGGATCT GCACCCAGAG ATCCATGCTC TCCTACAAAG TGCTGAGCTG 
CTTGAGGAAG AAGCACCCGA CATTCCCCTA AGGAGAGTCC ACAGCACTTC AAGTCTGGAT 
AGTCAGAAGT TTGTGACAGT GCCCTCCAGT AATGAAGTGG TAACTAACCT GGAAGTGCTG 
ACAAGCAAAT GCCTCCATGG GAAGAACTAC TGTCGACAGG TCCTCTGTCT GTATGATCTT 
GCCAAGGAGT TGGGCTGTTC CTACACAGAT GTTGCTGCTC AGGATGGTGA AGCCATGCTC 
CGGAAAATCT TGGCCTCTCA GCAGCCTGAC CGATGCAAAC GAGCCCAGGC CTTCATCAGC 
ACACAGGGCC TTAAGCCAGA TACTGTGGCT GAACTCGTGG CAGAAGAGGT GACACGGGAG 
CTGCTTACTT CATCACAGGG AACAGGACAT AAGCAGATGT TCAACCCAAC AGAGGAAAGC 
CAGACATTTC TTCAGCTGAC CACTCTGTGT CAAGACCGCA CATTGGTAGG CATGAAGTTG 
TTGGATAAGA TTTCCTCCGT TCCCCATGGG GAACTGTCTT GCACCACAGA GCTCCTGATC 
CTGGCCCATC ATTGCTTCAC CCTGACGTGC CACATGGAGG GCATCATCCG AGTCCTACAG 
GCCGCCCACA TGCTCACAGA TAACCACCTG GCCCCCAGTG AGGAGTATGG GCTGGTGGTA 
CGGCTCCTCA CTGGCATTGG AAGGTACAAC GAGATGACAT ACATATTTGA TTTGCTGCAT 
AAAAAGCACT ACTTTGAAGT GCTAATGAGG AAGAAGTTGG ATCCGAGTGG TACCCTGAAA 
ACAGCCCTGC TGGACTACAT CAAACGCTGC CGTCCTGGAG ACAGTGAAAA GCACAATATG 
ATTGCCCTGT GCTTCAGCAT GTGCCGGGAG ATTGGCGAGA ACCACGAGGC AGCTGCCCGC 
ATCCAACTGA AATTGATTGA GTCTCAGCCC TGGGAGGACA GCCTCAAGGA TGGGCACCAG 
CTGAAACAAC TGCTGCTGAA GGCCCTGACT CTGATGTTGG ATGCAGCAGA GAGTTATGCC 
AAGGACTCCT GTGTGCGACA GGCCCAGCAC TGTCAGCGGC TCACCAAGTT GATAACTCTG 
CAGATTCACT TTCTGAACAC TGGCCAGAAC ACAATGCTCA TCAACTTGGG CCGCCACAAG 
CTGATGGACT GTATTCTGGC CCTACCTCGG TTCTACCAGG CTTCTATTGT GGCTGAGGCC 
TACGATTTTG TTCCAGATTG GGCTGAAATT TTATACCAGC AAGTGATTCT TAAAGGAGAC 
TTTAATTACT TGGAAGAATT TAAGCAGCAA AGGTTATTAA AGTCCAGTAT ATTTGAAGAG 
ATTTCCAAAA AATATAAACA ACATCAGCCT ACTGACATGG TCATGGAAAA CCTGAAGAAA 
TTACTCACAT ATTGTGAAGA TGTTTACCTG TATTACAAGT TGGCATACGA ACACAAGTTT 
TATGAAATTG TAAATGTGCT TCTGAAGGAC CCTCAGACAG GTTGCTGTCT AAAGGACATG 
CTAGCAGGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079413.3
CDS	16..7347
Translation	MAAEEGVASAASAGGSWGTAAMGRVLPMLLVPVPAEAMGQLGSRAQLRTQPEALGSLTAAGSLQVLSLTPGSRGGGRCCLEGPFWHFLWEDSRNSSTPTEKPKLLALGENYELLIYEFNLKDGRCDATILYSCSREALQKLIDDQDISISLLSLRILSFHNNTSLLFINKCVILHIIFPERDAAIRVLNCFTLPLPAQAVDMIIDTQLCRGILFVLSSLGWIYIFDVVDGTYVAHVDLALHKEDMCNEQQQEPAKISSFTSLKVSQDLDVAVIVSSSNSAVALNLNLYFRQHPGHLLCERILEDLPIQGPKGVDEDDPVNSAYNMKLAKFSFQIDRSWKAQLSSLNETIKNSKLEVSCCAPWFQDILHLESPESGNHSTSVQSWAFIPQDIMHGQYNVLQKDHAKTSDPGRSWKIMHISEQEEPIELKCVSVTGFTALFTWEVERMGYTITLWDLETQGMQCFSLGTKCIPVDSSGDQQLCFVLTENGLSLILFGLTQEEFLNRLMIHGSASTVDTLCHLNGWGRCSIPIHALEAGIENRQLDTVNFFLKSKENLFNPSSKSSVSDQFDHLSSHLYLRNVEELIPALDLLCSAIRESYSEPQSKHFSEQLLNLTLSFLNNQIKELFIHTEELDEHLQKGVNILTSYINELRTFMIKFPWKLTDAIDEYDVHENVPKVKESNIWKKLSFEEVIASAILNNKIPEAQTFFRIDSHSAQKLEELIGIGLNLVFDNLKKNNIKEASELLKNMGFDVKGQLLKICFYTTNKNIRDFLVEILKEKNYFSEKEKRTIDFVHQVEKLYLGHFQENMQIQSFPRYWIKEQDFFKHKSVLDSFLKYDCKDEFNKQDHRIVLNWALWWDQLTQESILLPRISPEEYKSYSPEALWRYLTARHDWLNIILWIGEFQTQHSYASLQQNKWPLLTVDVINQNTSCNNYMRNEILDKLARNGVFLASELEDFECFLLRLSRIGGVIQDTLPVQNYKTKEGWDFHSQFILYCLEHSLQHLLYVYLDCYKLSPENCPFLEKKELHEAHPWFEFLVQCRQVASNLTDPKLIFQASLANAQILIPTNQASVSSMLLEGHTLLALATTMYSPGGVSQVVQNEENENCLKKVDPQLLKMALTPYPKLKTALFPQCTPPSVLPSDITIYHLIQSLSPFDPSRLFGWQSANTLAIGDAWSHLPHFSSPDLVNKYAIVERLNFAYYLHNGRPSFAFGTFLVQELIKSKTPKQLIQQVGNEAYVIGLSSFHIPSIGAACVCFLELLGLDSLKLRVDMKVANIILSYKCRNEDAQYSFIRESVAEKLSKLADGEKTTTEELLVLLEEGTWNSIQQQEIKRLSSESSSQWALVVQFCRLHNMKLSISYLRECAKANDWLQFIIHSQLHNYHPAEVKSLIQYFSPVIQDHLRLAFENLPSVPTSKMDSDQVCNKCPQELQGSKQEMTDLFEILLQCSEEPDSWHWLLVEAVKQQAPILSVLASCLQGASAISCLCVWIITSVEDNVATEAMGHIQDSTEDHTWNLEDLSVIWRTLLTRQKSKTLIRGFQLFFKDSPLLLVMEMYELCMFFRNYKEAEAKLLEFQKSLETLNTAATKVHPVIPAMWLEDQVCFLLKLMLQQCKTQYELGKLLQLFVEREHLFSDGPDVKKLCILCQILKDTSIAINHTIITSYSIENLQHECRSILERLQTDGQFALARRVAELAELPVDNLVIKEITQEMQTLKHIEQWSLKQARIDFWKKCHENFKKNSISSKAASSFFSTQAHVACEHPTGWSSMEERHLLLTLAGHWLAQEDVVPLDKLEELEKQIWLCRITQHTLGRNQEETEPRFSRQISTSGELSFDSLASEFSFSKLAALNTSKYLELNSLPSKETCENRLDWKEQESLNFLIGRLLDDGCVHEASRVCRYFHFYNPDVALVLHCRALASGEASMEDLHPEIHALLQSAELLEEEAPDIPLRRVHSTSSLDSQKFVTVPSSNEVVTNLEVLTSKCLHGKNYCRQVLCLYDLAKELGCSYTDVAAQDGEAMLRKILASQQPDRCKRAQAFISTQGLKPDTVAELVAEEVTRELLTSSQGTGHKQMFNPTEESQTFLQLTTLCQDRTLVGMKLLDKISSVPHGELSCTTELLILAHHCFTLTCHMEGIIRVLQAAHMLTDNHLAPSEEYGLVVRLLTGIGRYNEMTYIFDLLHKKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAARIQLKLIESQPWEDSLKDGHQLKQLLLKALTLMLDAAESYAKDSCVRQAQHCQRLTKLITLQIHFLNTGQNTMLINLGRHKLMDCILALPRFYQASIVAEAYDFVPDWAEILYQQVILKGDFNYLEEFKQQRLLKSSIFEEISKKYKQHQPTDMVMENLKKLLTYCEDVYLYYKLAYEHKFYEIVNVLLKDPQTGCCLKDMLAG

Target ORF information:

RefSeq Version	NM_025137.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025137.4

ORF Insert Sequence:

ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG 
CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG 
GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG 
GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA 
AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG 
AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG 
CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC 
AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA 
AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG 
GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC 
TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT 
CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT 
TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA 
GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA 
ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC 
TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC 
CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT 
CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT 
GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG 
AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA 
CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT 
TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG 
CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG 
TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG 
TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC 
AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT 
CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA 
AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG 
GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA 
CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC 
CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG 
AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG 
CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC 
AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA 
ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG 
CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA 
GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC 
TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT 
TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT 
TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA 
CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT 
GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA 
ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT 
GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT 
GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG 
ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA 
GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC 
CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC 
AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT 
CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC 
TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT 
CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT 
GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT 
ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG 
AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA 
ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG 
CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA 
TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA 
CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT 
TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA 
ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA 
GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG 
CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC 
TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA 
CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA 
GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC 
AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT 
TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC 
CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA 
GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC 
GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT 
TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT 
GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC 
AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT 
GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT 
TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC 
CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG 
TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA 
ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT 
CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG 
AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA 
AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT 
ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG 
CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG 
GACAACTTGG TTATTAAAGA GATAACACAG GAAATGCAGA CCCTAAAACA CATTGAACAG 
TGGTCACTAA AACAAGCAAG AATTGACTTC TGGAAAAAAT GCCATGAGAA TTTTAAGAAA 
AATTCAATTT CAAGCAAAGC AGCTTCTTCC TTTTTCTCAA CCCAGGCCCA TGTGGCATGT 
GAGCACCCAA CTGGATGGAG CAGCATGGAG GAGCGCCATC TGCTGCTCAC CTTGGCAGGG 
CACTGGCTTG CCCAGGAGGA CGTGGTGCCC TTGGATAAGC TGGAGGAGCT GGAGAAGCAG 
ATCTGGCTGT GCCGCATCAC CCAGCACACT CTTGGAAGAA ATCAGGAGGA AACAGAGCCC 
AGATTTTCTC GACAGATCTC AACTAGTGGT GAACTTTCCT TTGATAGTTT AGCCAGTGAG 
TTTTCCTTCT CCAAGTTGGC TGCTCTGAAC ACATCAAAAT ACTTAGAACT TAACAGCCTT 
CCATCCAAAG AGACATGCGA GAATAGATTG GATTGGAAAG AGCAGGAGTC ACTAAACTTT 
TTGATTGGGC GCCTACTGGA TGATGGCTGT GTGCATGAAG CAAGTAGAGT ATGCCGGTAT 
TTTCATTTTT ATAATCCAGA TGTCGCCTTG GTATTGCACT GCAGAGCACT GGCCTCAGGG 
GAAGCTAGTA TGGAGGATCT GCACCCAGAG ATCCATGCTC TCCTACAAAG TGCTGAGCTG 
CTTGAGGAAG AAGCACCCGA CATTCCCCTA AGGAGAGTCC ACAGCACTTC AAGTCTGGAT 
AGTCAGAAGT TTGTGACAGT GCCCTCCAGT AATGAAGTGG TAACTAACCT GGAAGTGCTG 
ACAAGCAAAT GCCTCCATGG GAAGAACTAC TGTCGACAGG TCCTCTGTCT GTATGATCTT 
GCCAAGGAGT TGGGCTGTTC CTACACAGAT GTTGCTGCTC AGGATGGTGA AGCCATGCTC 
CGGAAAATCT TGGCCTCTCA GCAGCCTGAC CGATGCAAAC GAGCCCAGGC CTTCATCAGC 
ACACAGGGCC TTAAGCCAGA TACTGTGGCT GAACTCGTGG CAGAAGAGGT GACACGGGAG 
CTGCTTACTT CATCACAGGG AACAGGACAT AAGCAGATGT TCAACCCAAC AGAGGAAAGC 
CAGACATTTC TTCAGCTGAC CACTCTGTGT CAAGACCGCA CATTGGTAGG CATGAAGTTG 
TTGGATAAGA TTTCCTCCGT TCCCCATGGG GAACTGTCTT GCACCACAGA GCTCCTGATC 
CTGGCCCATC ATTGCTTCAC CCTGACGTGC CACATGGAGG GCATCATCCG AGTCCTACAG 
GCCGCCCACA TGCTCACAGA TAACCACCTG GCCCCCAGTG AGGAGTATGG GCTGGTGGTA 
CGGCTCCTCA CTGGCATTGG AAGGTACAAC GAGATGACAT ACATATTTGA TTTGCTGCAT 
AAAAAGCACT ACTTTGAAGT GCTAATGAGG AAGAAGTTGG ATCCGAGTGG TACCCTGAAA 
ACAGCCCTGC TGGACTACAT CAAACGCTGC CGTCCTGGAG ACAGTGAAAA GCACAATATG 
ATTGCCCTGT GCTTCAGCAT GTGCCGGGAG ATTGGCGAGA ACCACGAGGC AGCTGCCCGC 
ATCCAACTGA AATTGATTGA GTCTCAGCCC TGGGAGGACA GCCTCAAGGA TGGGCACCAG 
CTGAAACAAC TGCTGCTGAA GGCCCTGACT CTGATGTTGG ATGCAGCAGA GAGTTATGCC 
AAGGACTCCT GTGTGCGACA GGCCCAGCAC TGTCAGCGGC TCACCAAGTT GATAACTCTG 
CAGATTCACT TTCTGAACAC TGGCCAGAAC ACAATGCTCA TCAACTTGGG CCGCCACAAG 
CTGATGGACT GTATTCTGGC CCTACCTCGG TTCTACCAGG CTTCTATTGT GGCTGAGGCC 
TACGATTTTG TTCCAGATTG GGCTGAAATT TTATACCAGC AAGTGATTCT TAAAGGAGAC 
TTTAATTACT TGGAAGAATT TAAGCAGCAA AGGTTATTAA AGTCCAGTAT ATTTGAAGAG 
ATTTCCAAAA AATATAAACA ACATCAGCCT ACTGACATGG TCATGGAAAA CCTGAAGAAA 
TTACTCACAT ATTGTGAAGA TGTTTACCTG TATTACAAGT TGGCATACGA ACACAAGTTT 
TATGAAATTG TAAATGTGCT TCTGAAGGAC CCTCAGACAG GTTGCTGTCT AAAGGACATG 
CTAGCAGGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( NM_025137.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_079413.3 Homo sapiens SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant 1, mRNA.

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CloneID	OHu19157
Clone ID Related Accession (Same CDS sequence)	NM_025137.4 , NM_025137.3
Accession Version	NM_025137.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	spatacsin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009996.7, AB058743.2 and BC094704.1. This sequence is a reference standard in the RefSeqGene project. On Apr 22, 2006 this sequence version replaced NM_025137.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC153879.1, AB058743.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG 
CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG 
GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG 
GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA 
AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG 
AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG 
CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC 
AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA 
AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG 
GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC 
TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT 
CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT 
TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA 
GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA 
ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC 
TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC 
CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT 
CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT 
GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG 
AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA 
CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT 
TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG 
CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG 
TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG 
TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC 
AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT 
CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA 
AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG 
GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA 
CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC 
CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG 
AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG 
CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC 
AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA 
ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG 
CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA 
GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC 
TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT 
TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT 
TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA 
CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT 
GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA 
ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT 
GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT 
GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG 
ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA 
GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC 
CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC 
AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT 
CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC 
TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT 
CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT 
GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT 
ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG 
AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA 
ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG 
CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA 
TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA 
CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT 
TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA 
ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA 
GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG 
CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC 
TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA 
CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA 
GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC 
AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT 
TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC 
CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA 
GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC 
GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT 
TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT 
GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC 
AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT 
GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT 
TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC 
CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG 
TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA 
ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT 
CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG 
AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA 
AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT 
ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG 
CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG 
GACAACTTGG TTATTAAAGA GATAACACAG GAAATGCAGA CCCTAAAACA CATTGAACAG 
TGGTCACTAA AACAAGCAAG AATTGACTTC TGGAAAAAAT GCCATGAGAA TTTTAAGAAA 
AATTCAATTT CAAGCAAAGC AGCTTCTTCC TTTTTCTCAA CCCAGGCCCA TGTGGCATGT 
GAGCACCCAA CTGGATGGAG CAGCATGGAG GAGCGCCATC TGCTGCTCAC CTTGGCAGGG 
CACTGGCTTG CCCAGGAGGA CGTGGTGCCC TTGGATAAGC TGGAGGAGCT GGAGAAGCAG 
ATCTGGCTGT GCCGCATCAC CCAGCACACT CTTGGAAGAA ATCAGGAGGA AACAGAGCCC 
AGATTTTCTC GACAGATCTC AACTAGTGGT GAACTTTCCT TTGATAGTTT AGCCAGTGAG 
TTTTCCTTCT CCAAGTTGGC TGCTCTGAAC ACATCAAAAT ACTTAGAACT TAACAGCCTT 
CCATCCAAAG AGACATGCGA GAATAGATTG GATTGGAAAG AGCAGGAGTC ACTAAACTTT 
TTGATTGGGC GCCTACTGGA TGATGGCTGT GTGCATGAAG CAAGTAGAGT ATGCCGGTAT 
TTTCATTTTT ATAATCCAGA TGTCGCCTTG GTATTGCACT GCAGAGCACT GGCCTCAGGG 
GAAGCTAGTA TGGAGGATCT GCACCCAGAG ATCCATGCTC TCCTACAAAG TGCTGAGCTG 
CTTGAGGAAG AAGCACCCGA CATTCCCCTA AGGAGAGTCC ACAGCACTTC AAGTCTGGAT 
AGTCAGAAGT TTGTGACAGT GCCCTCCAGT AATGAAGTGG TAACTAACCT GGAAGTGCTG 
ACAAGCAAAT GCCTCCATGG GAAGAACTAC TGTCGACAGG TCCTCTGTCT GTATGATCTT 
GCCAAGGAGT TGGGCTGTTC CTACACAGAT GTTGCTGCTC AGGATGGTGA AGCCATGCTC 
CGGAAAATCT TGGCCTCTCA GCAGCCTGAC CGATGCAAAC GAGCCCAGGC CTTCATCAGC 
ACACAGGGCC TTAAGCCAGA TACTGTGGCT GAACTCGTGG CAGAAGAGGT GACACGGGAG 
CTGCTTACTT CATCACAGGG AACAGGACAT AAGCAGATGT TCAACCCAAC AGAGGAAAGC 
CAGACATTTC TTCAGCTGAC CACTCTGTGT CAAGACCGCA CATTGGTAGG CATGAAGTTG 
TTGGATAAGA TTTCCTCCGT TCCCCATGGG GAACTGTCTT GCACCACAGA GCTCCTGATC 
CTGGCCCATC ATTGCTTCAC CCTGACGTGC CACATGGAGG GCATCATCCG AGTCCTACAG 
GCCGCCCACA TGCTCACAGA TAACCACCTG GCCCCCAGTG AGGAGTATGG GCTGGTGGTA 
CGGCTCCTCA CTGGCATTGG AAGGTACAAC GAGATGACAT ACATATTTGA TTTGCTGCAT 
AAAAAGCACT ACTTTGAAGT GCTAATGAGG AAGAAGTTGG ATCCGAGTGG TACCCTGAAA 
ACAGCCCTGC TGGACTACAT CAAACGCTGC CGTCCTGGAG ACAGTGAAAA GCACAATATG 
ATTGCCCTGT GCTTCAGCAT GTGCCGGGAG ATTGGCGAGA ACCACGAGGC AGCTGCCCGC 
ATCCAACTGA AATTGATTGA GTCTCAGCCC TGGGAGGACA GCCTCAAGGA TGGGCACCAG 
CTGAAACAAC TGCTGCTGAA GGCCCTGACT CTGATGTTGG ATGCAGCAGA GAGTTATGCC 
AAGGACTCCT GTGTGCGACA GGCCCAGCAC TGTCAGCGGC TCACCAAGTT GATAACTCTG 
CAGATTCACT TTCTGAACAC TGGCCAGAAC ACAATGCTCA TCAACTTGGG CCGCCACAAG 
CTGATGGACT GTATTCTGGC CCTACCTCGG TTCTACCAGG CTTCTATTGT GGCTGAGGCC 
TACGATTTTG TTCCAGATTG GGCTGAAATT TTATACCAGC AAGTGATTCT TAAAGGAGAC 
TTTAATTACT TGGAAGAATT TAAGCAGCAA AGGTTATTAA AGTCCAGTAT ATTTGAAGAG 
ATTTCCAAAA AATATAAACA ACATCAGCCT ACTGACATGG TCATGGAAAA CCTGAAGAAA 
TTACTCACAT ATTGTGAAGA TGTTTACCTG TATTACAAGT TGGCATACGA ACACAAGTTT 
TATGAAATTG TAAATGTGCT TCTGAAGGAC CCTCAGACAG GTTGCTGTCT AAAGGACATG 
CTAGCAGGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079413.3
CDS	32..7363
Translation	MAAEEGVASAASAGGSWGTAAMGRVLPMLLVPVPAEAMGQLGSRAQLRTQPEALGSLTAAGSLQVLSLTPGSRGGGRCCLEGPFWHFLWEDSRNSSTPTEKPKLLALGENYELLIYEFNLKDGRCDATILYSCSREALQKLIDDQDISISLLSLRILSFHNNTSLLFINKCVILHIIFPERDAAIRVLNCFTLPLPAQAVDMIIDTQLCRGILFVLSSLGWIYIFDVVDGTYVAHVDLALHKEDMCNEQQQEPAKISSFTSLKVSQDLDVAVIVSSSNSAVALNLNLYFRQHPGHLLCERILEDLPIQGPKGVDEDDPVNSAYNMKLAKFSFQIDRSWKAQLSSLNETIKNSKLEVSCCAPWFQDILHLESPESGNHSTSVQSWAFIPQDIMHGQYNVLQKDHAKTSDPGRSWKIMHISEQEEPIELKCVSVTGFTALFTWEVERMGYTITLWDLETQGMQCFSLGTKCIPVDSSGDQQLCFVLTENGLSLILFGLTQEEFLNRLMIHGSASTVDTLCHLNGWGRCSIPIHALEAGIENRQLDTVNFFLKSKENLFNPSSKSSVSDQFDHLSSHLYLRNVEELIPALDLLCSAIRESYSEPQSKHFSEQLLNLTLSFLNNQIKELFIHTEELDEHLQKGVNILTSYINELRTFMIKFPWKLTDAIDEYDVHENVPKVKESNIWKKLSFEEVIASAILNNKIPEAQTFFRIDSHSAQKLEELIGIGLNLVFDNLKKNNIKEASELLKNMGFDVKGQLLKICFYTTNKNIRDFLVEILKEKNYFSEKEKRTIDFVHQVEKLYLGHFQENMQIQSFPRYWIKEQDFFKHKSVLDSFLKYDCKDEFNKQDHRIVLNWALWWDQLTQESILLPRISPEEYKSYSPEALWRYLTARHDWLNIILWIGEFQTQHSYASLQQNKWPLLTVDVINQNTSCNNYMRNEILDKLARNGVFLASELEDFECFLLRLSRIGGVIQDTLPVQNYKTKEGWDFHSQFILYCLEHSLQHLLYVYLDCYKLSPENCPFLEKKELHEAHPWFEFLVQCRQVASNLTDPKLIFQASLANAQILIPTNQASVSSMLLEGHTLLALATTMYSPGGVSQVVQNEENENCLKKVDPQLLKMALTPYPKLKTALFPQCTPPSVLPSDITIYHLIQSLSPFDPSRLFGWQSANTLAIGDAWSHLPHFSSPDLVNKYAIVERLNFAYYLHNGRPSFAFGTFLVQELIKSKTPKQLIQQVGNEAYVIGLSSFHIPSIGAACVCFLELLGLDSLKLRVDMKVANIILSYKCRNEDAQYSFIRESVAEKLSKLADGEKTTTEELLVLLEEGTWNSIQQQEIKRLSSESSSQWALVVQFCRLHNMKLSISYLRECAKANDWLQFIIHSQLHNYHPAEVKSLIQYFSPVIQDHLRLAFENLPSVPTSKMDSDQVCNKCPQELQGSKQEMTDLFEILLQCSEEPDSWHWLLVEAVKQQAPILSVLASCLQGASAISCLCVWIITSVEDNVATEAMGHIQDSTEDHTWNLEDLSVIWRTLLTRQKSKTLIRGFQLFFKDSPLLLVMEMYELCMFFRNYKEAEAKLLEFQKSLETLNTAATKVHPVIPAMWLEDQVCFLLKLMLQQCKTQYELGKLLQLFVEREHLFSDGPDVKKLCILCQILKDTSIAINHTIITSYSIENLQHECRSILERLQTDGQFALARRVAELAELPVDNLVIKEITQEMQTLKHIEQWSLKQARIDFWKKCHENFKKNSISSKAASSFFSTQAHVACEHPTGWSSMEERHLLLTLAGHWLAQEDVVPLDKLEELEKQIWLCRITQHTLGRNQEETEPRFSRQISTSGELSFDSLASEFSFSKLAALNTSKYLELNSLPSKETCENRLDWKEQESLNFLIGRLLDDGCVHEASRVCRYFHFYNPDVALVLHCRALASGEASMEDLHPEIHALLQSAELLEEEAPDIPLRRVHSTSSLDSQKFVTVPSSNEVVTNLEVLTSKCLHGKNYCRQVLCLYDLAKELGCSYTDVAAQDGEAMLRKILASQQPDRCKRAQAFISTQGLKPDTVAELVAEEVTRELLTSSQGTGHKQMFNPTEESQTFLQLTTLCQDRTLVGMKLLDKISSVPHGELSCTTELLILAHHCFTLTCHMEGIIRVLQAAHMLTDNHLAPSEEYGLVVRLLTGIGRYNEMTYIFDLLHKKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAARIQLKLIESQPWEDSLKDGHQLKQLLLKALTLMLDAAESYAKDSCVRQAQHCQRLTKLITLQIHFLNTGQNTMLINLGRHKLMDCILALPRFYQASIVAEAYDFVPDWAEILYQQVILKGDFNYLEEFKQQRLLKSSIFEEISKKYKQHQPTDMVMENLKKLLTYCEDVYLYYKLAYEHKFYEIVNVLLKDPQTGCCLKDMLAG

Target ORF information:

RefSeq Version	NM_025137.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SPG11, spatacsin vesicle trafficking associated (SPG11), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025137.3

ORF Insert Sequence:

ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG 
CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG 
GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG 
GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA 
AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG 
AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG 
CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC 
AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA 
AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG 
GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC 
TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT 
CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT 
TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA 
GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA 
ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC 
TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC 
CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT 
CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT 
GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG 
AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA 
CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT 
TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG 
CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG 
TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG 
TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC 
AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT 
CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA 
AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG 
GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA 
CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC 
CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG 
AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG 
CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC 
AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA 
ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG 
CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA 
GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC 
TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT 
TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT 
TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA 
CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT 
GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA 
ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT 
GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT 
GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG 
ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA 
GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC 
CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC 
AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT 
CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC 
TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT 
CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT 
GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT 
ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG 
AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA 
ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG 
CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA 
TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA 
CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT 
TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA 
ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA 
GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG 
CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC 
TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA 
CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA 
GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC 
AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT 
TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC 
CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA 
GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC 
GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT 
TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT 
GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC 
AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT 
GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT 
TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC 
CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG 
TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA 
ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT 
CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG 
AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA 
AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT 
ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG 
CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG 
GACAACTTGG TTATTAAAGA GATAACACAG GAAATGCAGA CCCTAAAACA CATTGAACAG 
TGGTCACTAA AACAAGCAAG AATTGACTTC TGGAAAAAAT GCCATGAGAA TTTTAAGAAA 
AATTCAATTT CAAGCAAAGC AGCTTCTTCC TTTTTCTCAA CCCAGGCCCA TGTGGCATGT 
GAGCACCCAA CTGGATGGAG CAGCATGGAG GAGCGCCATC TGCTGCTCAC CTTGGCAGGG 
CACTGGCTTG CCCAGGAGGA CGTGGTGCCC TTGGATAAGC TGGAGGAGCT GGAGAAGCAG 
ATCTGGCTGT GCCGCATCAC CCAGCACACT CTTGGAAGAA ATCAGGAGGA AACAGAGCCC 
AGATTTTCTC GACAGATCTC AACTAGTGGT GAACTTTCCT TTGATAGTTT AGCCAGTGAG 
TTTTCCTTCT CCAAGTTGGC TGCTCTGAAC ACATCAAAAT ACTTAGAACT TAACAGCCTT 
CCATCCAAAG AGACATGCGA GAATAGATTG GATTGGAAAG AGCAGGAGTC ACTAAACTTT 
TTGATTGGGC GCCTACTGGA TGATGGCTGT GTGCATGAAG CAAGTAGAGT ATGCCGGTAT 
TTTCATTTTT ATAATCCAGA TGTCGCCTTG GTATTGCACT GCAGAGCACT GGCCTCAGGG 
GAAGCTAGTA TGGAGGATCT GCACCCAGAG ATCCATGCTC TCCTACAAAG TGCTGAGCTG 
CTTGAGGAAG AAGCACCCGA CATTCCCCTA AGGAGAGTCC ACAGCACTTC AAGTCTGGAT 
AGTCAGAAGT TTGTGACAGT GCCCTCCAGT AATGAAGTGG TAACTAACCT GGAAGTGCTG 
ACAAGCAAAT GCCTCCATGG GAAGAACTAC TGTCGACAGG TCCTCTGTCT GTATGATCTT 
GCCAAGGAGT TGGGCTGTTC CTACACAGAT GTTGCTGCTC AGGATGGTGA AGCCATGCTC 
CGGAAAATCT TGGCCTCTCA GCAGCCTGAC CGATGCAAAC GAGCCCAGGC CTTCATCAGC 
ACACAGGGCC TTAAGCCAGA TACTGTGGCT GAACTCGTGG CAGAAGAGGT GACACGGGAG 
CTGCTTACTT CATCACAGGG AACAGGACAT AAGCAGATGT TCAACCCAAC AGAGGAAAGC 
CAGACATTTC TTCAGCTGAC CACTCTGTGT CAAGACCGCA CATTGGTAGG CATGAAGTTG 
TTGGATAAGA TTTCCTCCGT TCCCCATGGG GAACTGTCTT GCACCACAGA GCTCCTGATC 
CTGGCCCATC ATTGCTTCAC CCTGACGTGC CACATGGAGG GCATCATCCG AGTCCTACAG 
GCCGCCCACA TGCTCACAGA TAACCACCTG GCCCCCAGTG AGGAGTATGG GCTGGTGGTA 
CGGCTCCTCA CTGGCATTGG AAGGTACAAC GAGATGACAT ACATATTTGA TTTGCTGCAT 
AAAAAGCACT ACTTTGAAGT GCTAATGAGG AAGAAGTTGG ATCCGAGTGG TACCCTGAAA 
ACAGCCCTGC TGGACTACAT CAAACGCTGC CGTCCTGGAG ACAGTGAAAA GCACAATATG 
ATTGCCCTGT GCTTCAGCAT GTGCCGGGAG ATTGGCGAGA ACCACGAGGC AGCTGCCCGC 
ATCCAACTGA AATTGATTGA GTCTCAGCCC TGGGAGGACA GCCTCAAGGA TGGGCACCAG 
CTGAAACAAC TGCTGCTGAA GGCCCTGACT CTGATGTTGG ATGCAGCAGA GAGTTATGCC 
AAGGACTCCT GTGTGCGACA GGCCCAGCAC TGTCAGCGGC TCACCAAGTT GATAACTCTG 
CAGATTCACT TTCTGAACAC TGGCCAGAAC ACAATGCTCA TCAACTTGGG CCGCCACAAG 
CTGATGGACT GTATTCTGGC CCTACCTCGG TTCTACCAGG CTTCTATTGT GGCTGAGGCC 
TACGATTTTG TTCCAGATTG GGCTGAAATT TTATACCAGC AAGTGATTCT TAAAGGAGAC 
TTTAATTACT TGGAAGAATT TAAGCAGCAA AGGTTATTAA AGTCCAGTAT ATTTGAAGAG 
ATTTCCAAAA AATATAAACA ACATCAGCCT ACTGACATGG TCATGGAAAA CCTGAAGAAA 
TTACTCACAT ATTGTGAAGA TGTTTACCTG TATTACAAGT TGGCATACGA ACACAAGTTT 
TATGAAATTG TAAATGTGCT TCTGAAGGAC CCTCAGACAG GTTGCTGTCT AAAGGACATG 
CTAGCAGGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPG11 ( XM_006720700.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006720763.1 Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X2, mRNA.

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CloneID	OHu48595
Clone ID Related Accession (Same CDS sequence)	XM_006720700.1
Accession Version	XM_006720700.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spatacsin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG 
CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG 
GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG 
GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA 
AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG 
AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG 
CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC 
AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA 
AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG 
GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC 
TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT 
CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT 
TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA 
GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA 
ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC 
TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC 
CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT 
CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT 
GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG 
AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA 
CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT 
TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG 
CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG 
TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG 
TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC 
AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT 
CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA 
AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG 
GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA 
CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC 
CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG 
AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG 
CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC 
AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA 
ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG 
CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA 
GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC 
TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT 
TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT 
TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA 
CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT 
GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA 
ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT 
GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT 
GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG 
ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA 
GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC 
CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC 
AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT 
CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC 
TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT 
CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT 
GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT 
ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG 
AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA 
ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG 
CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA 
TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA 
CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT 
TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA 
ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA 
GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG 
CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC 
TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA 
CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA 
GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC 
AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT 
TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC 
CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA 
GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC 
GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT 
TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT 
GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC 
AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT 
GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT 
TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC 
CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG 
TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA 
ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT 
CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG 
AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA 
AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT 
ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG 
CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG 
GACAACTTGG TTATTAAAGA GGCCCATGTG GCATGTGAGC ACCCAACTGG ATGGAGCAGC 
ATGGAGGAGC GCCATCTGCT GCTCACCTTG GCAGGGCACT GGCTTGCCCA GGAGGACGTG 
GTGCCCTTGG ATAAGCTGGA GGAGCTGGAG AAGCAGATCT GGCTGTGCCG CATCACCCAG 
CACACTCTTG GAAGAAATCA GGAGGAAACA GAGCCCAGAT TTTCTCGACA GATCTCAACT 
AGTGGTGAAC TTTCCTTTGA TAGTTTAGCC AGTGAGTTTT CCTTCTCCAA GTTGGCTGCT 
CTGAACACAT CAAAATACTT AGAACTTAAC AGCCTTCCAT CCAAAGAGAC ATGCGAGAAT 
AGATTGGATT GGAAAGAGCA GGAGTCACTA AACTTTTTGA TTGGGCGCCT ACTGGATGAT 
GGCTGTGTGC ATGAAGCAAG TAGAGTATGC CGGTATTTTC ATTTTTATAA TCCAGATGTC 
GCCTTGGTAT TGCACTGCAG AGCACTGGCC TCAGGGGAAG CTAGTATGGA GGATCTGCAC 
CCAGAGATCC ATGCTCTCCT ACAAAGTGCT GAGCTGCTTG AGGAAGAAGC ACCCGACATT 
CCCCTAAGGA GAGTCCACAG CACTTCAAGT CTGGATAGTC AGAAGTTTGT GACAGTGCCC 
TCCAGTAATG AAGTGGTAAC TAACCTGGAA GTGCTGACAA GCAAATGCCT CCATGGGAAG 
AACTACTGTC GACAGGTCCT CTGTCTGTAT GATCTTGCCA AGGAGTTGGG CTGTTCCTAC 
ACAGATGTTG CTGCTCAGGA TGGTGAAGCC ATGCTCCGGA AAATCTTGGC CTCTCAGCAG 
CCTGACCGAT GCAAACGAGC CCAGGCCTTC ATCAGCACAC AGGGCCTTAA GCCAGATACT 
GTGGCTGAAC TCGTGGCAGA AGAGGTGACA CGGGAGCTGC TTACTTCATC ACAGGGAACA 
GGACATAAGC AGATGTTCAA CCCAACAGAG GAAAGCCAGA CATTTCTTCA GCTGACCACT 
CTGTGTCAAG ACCGCACATT GGTAGGCATG AAGTTGTTGG ATAAGATTTC CTCCGTTCCC 
CATGGGGAAC TGTCTTGCAC CACAGAGCTC CTGATCCTGG CCCATCATTG CTTCACCCTG 
ACGTGCCACA TGGAGGGCAT CATCCGAGTC CTACAGGCCG CCCACATGCT CACAGATAAC 
CACCTGGCCC CCAGTGAGGA GTATGGGCTG GTGGTACGGC TCCTCACTGG CATTGGAAGG 
TACAACGAGA TGACATACAT ATTTGATTTG CTGCATAAAA AGCACTACTT TGAAGTGCTA 
ATGAGGAAGA AGTTGGATCC GAGTGGTACC CTGAAAACAG CCCTGCTGGA CTACATCAAA 
CGCTGCCGTC CTGGAGACAG TGAAAAGCAC AATATGATTG CCCTGTGCTT CAGCATGTGC 
CGGGAGATTG GCGAGAACCA CGAGGCAGCT GCCCGCATCC AACTGAAATT GATTGAGTCT 
CAGCCCTGGG AGGACAGCCT CAAGGATGGG CACCAGCTGA AACAACTGCT GCTGAAGGCC 
CTGACTCTGA TGTTGGATGC AGCAGAGAGT TATGCCAAGG ACTCCTGTGT GCGACAGGCC 
CAGCACTGTC AGCGGCTCAC CAAGTTGATA ACTCTGCAGA TTCACTTTCT GAACACTGGC 
CAGAACACAA TGCTCATCAA CTTGGGCCGC CACAAGCTGA TGGACTGTAT TCTGGCCCTA 
CCTCGGTTCT ACCAGGCTTC TATTGTGGCT GAGGCCTACG ATTTTGTTCC AGATTGGGCT 
GAAATTTTAT ACCAGCAAGT GATTCTTAAA GGAGACTTTA ATTACTTGGA AGAATTTAAG 
CAGCAAAGGT TATTAAAGTC CAGTATATTT GAAGAGATTT CCAAAAAATA TAAACAACAT 
CAGCCTACTG ACATGGTCAT GGAAAACCTG AAGAAATTAC TCACATATTG TGAAGATGTT 
TACCTGTATT ACAAGTTGGC ATACGAACAC AAGTTTTATG AAATTGTAAA TGTGCTTCTG 
AAGGACCCTC AGACAGGTTG CTGTCTAAAG GACATGCTAG CAGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006720763.1
CDS	32..7219
Translation	MAAEEGVASAASAGGSWGTAAMGRVLPMLLVPVPAEAMGQLGSRAQLRTQPEALGSLTAAGSLQVLSLTPGSRGGGRCCLEGPFWHFLWEDSRNSSTPTEKPKLLALGENYELLIYEFNLKDGRCDATILYSCSREALQKLIDDQDISISLLSLRILSFHNNTSLLFINKCVILHIIFPERDAAIRVLNCFTLPLPAQAVDMIIDTQLCRGILFVLSSLGWIYIFDVVDGTYVAHVDLALHKEDMCNEQQQEPAKISSFTSLKVSQDLDVAVIVSSSNSAVALNLNLYFRQHPGHLLCERILEDLPIQGPKGVDEDDPVNSAYNMKLAKFSFQIDRSWKAQLSSLNETIKNSKLEVSCCAPWFQDILHLESPESGNHSTSVQSWAFIPQDIMHGQYNVLQKDHAKTSDPGRSWKIMHISEQEEPIELKCVSVTGFTALFTWEVERMGYTITLWDLETQGMQCFSLGTKCIPVDSSGDQQLCFVLTENGLSLILFGLTQEEFLNRLMIHGSASTVDTLCHLNGWGRCSIPIHALEAGIENRQLDTVNFFLKSKENLFNPSSKSSVSDQFDHLSSHLYLRNVEELIPALDLLCSAIRESYSEPQSKHFSEQLLNLTLSFLNNQIKELFIHTEELDEHLQKGVNILTSYINELRTFMIKFPWKLTDAIDEYDVHENVPKVKESNIWKKLSFEEVIASAILNNKIPEAQTFFRIDSHSAQKLEELIGIGLNLVFDNLKKNNIKEASELLKNMGFDVKGQLLKICFYTTNKNIRDFLVEILKEKNYFSEKEKRTIDFVHQVEKLYLGHFQENMQIQSFPRYWIKEQDFFKHKSVLDSFLKYDCKDEFNKQDHRIVLNWALWWDQLTQESILLPRISPEEYKSYSPEALWRYLTARHDWLNIILWIGEFQTQHSYASLQQNKWPLLTVDVINQNTSCNNYMRNEILDKLARNGVFLASELEDFECFLLRLSRIGGVIQDTLPVQNYKTKEGWDFHSQFILYCLEHSLQHLLYVYLDCYKLSPENCPFLEKKELHEAHPWFEFLVQCRQVASNLTDPKLIFQASLANAQILIPTNQASVSSMLLEGHTLLALATTMYSPGGVSQVVQNEENENCLKKVDPQLLKMALTPYPKLKTALFPQCTPPSVLPSDITIYHLIQSLSPFDPSRLFGWQSANTLAIGDAWSHLPHFSSPDLVNKYAIVERLNFAYYLHNGRPSFAFGTFLVQELIKSKTPKQLIQQVGNEAYVIGLSSFHIPSIGAACVCFLELLGLDSLKLRVDMKVANIILSYKCRNEDAQYSFIRESVAEKLSKLADGEKTTTEELLVLLEEGTWNSIQQQEIKRLSSESSSQWALVVQFCRLHNMKLSISYLRECAKANDWLQFIIHSQLHNYHPAEVKSLIQYFSPVIQDHLRLAFENLPSVPTSKMDSDQVCNKCPQELQGSKQEMTDLFEILLQCSEEPDSWHWLLVEAVKQQAPILSVLASCLQGASAISCLCVWIITSVEDNVATEAMGHIQDSTEDHTWNLEDLSVIWRTLLTRQKSKTLIRGFQLFFKDSPLLLVMEMYELCMFFRNYKEAEAKLLEFQKSLETLNTAATKVHPVIPAMWLEDQVCFLLKLMLQQCKTQYELGKLLQLFVEREHLFSDGPDVKKLCILCQILKDTSIAINHTIITSYSIENLQHECRSILERLQTDGQFALARRVAELAELPVDNLVIKEAHVACEHPTGWSSMEERHLLLTLAGHWLAQEDVVPLDKLEELEKQIWLCRITQHTLGRNQEETEPRFSRQISTSGELSFDSLASEFSFSKLAALNTSKYLELNSLPSKETCENRLDWKEQESLNFLIGRLLDDGCVHEASRVCRYFHFYNPDVALVLHCRALASGEASMEDLHPEIHALLQSAELLEEEAPDIPLRRVHSTSSLDSQKFVTVPSSNEVVTNLEVLTSKCLHGKNYCRQVLCLYDLAKELGCSYTDVAAQDGEAMLRKILASQQPDRCKRAQAFISTQGLKPDTVAELVAEEVTRELLTSSQGTGHKQMFNPTEESQTFLQLTTLCQDRTLVGMKLLDKISSVPHGELSCTTELLILAHHCFTLTCHMEGIIRVLQAAHMLTDNHLAPSEEYGLVVRLLTGIGRYNEMTYIFDLLHKKHYFEVLMRKKLDPSGTLKTALLDYIKRCRPGDSEKHNMIALCFSMCREIGENHEAAARIQLKLIESQPWEDSLKDGHQLKQLLLKALTLMLDAAESYAKDSCVRQAQHCQRLTKLITLQIHFLNTGQNTMLINLGRHKLMDCILALPRFYQASIVAEAYDFVPDWAEILYQQVILKGDFNYLEEFKQQRLLKSSIFEEISKKYKQHQPTDMVMENLKKLLTYCEDVYLYYKLAYEHKFYEIVNVLLKDPQTGCCLKDMLAG

Target ORF information:

RefSeq Version	XM_006720700.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SPG11 vesicle trafficking associated, spatacsin (SPG11), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006720700.1

ORF Insert Sequence:

ATGGCTGCAG AGGAAGGGGT CGCGAGTGCT GCTTCCGCCG GCGGTAGCTG GGGCACCGCG 
GCCATGGGGC GGGTTCTACC GATGCTGTTG GTGCCAGTCC CCGCCGAGGC GATGGGGCAG 
CTCGGCTCCC GGGCGCAGCT GCGCACACAG CCGGAGGCTC TGGGGAGCCT GACGGCTGCG 
GGCAGCCTCC AAGTGCTTTC TTTGACGCCT GGCAGCCGGG GCGGGGGTCG CTGCTGCCTG 
GAGGGCCCCT TCTGGCACTT TCTATGGGAG GATTCTCGTA ACAGCAGCAC ACCAACTGAA 
AAGCCCAAAC TGCTCGCTCT TGGTGAAAAT TATGAACTGC TTATCTATGA ATTTAATTTG 
AAAGATGGAA GATGTGATGC AACCATTTTG TATAGCTGTA GTAGGGAGGC ATTGCAAAAG 
CTCATTGACG ATCAAGATAT CAGTATTTCC TTATTGTCTT TGAGAATCCT GTCATTTCAC 
AATAACACAT CATTACTGTT CATCAACAAA TGTGTCATCC TACATATTAT ATTTCCTGAA 
AGAGATGCTG CAATTAGAGT ACTCAACTGT TTCACACTTC CCTTGCCTGC ACAGGCAGTG 
GACATGATTA TTGACACGCA GCTCTGCAGA GGAATTCTTT TTGTTTTGAG TAGTTTAGGC 
TGGATCTACA TTTTTGATGT TGTGGATGGT ACATATGTAG CTCATGTGGA TTTAGCACTT 
CACAAAGAAG ACATGTGTAA TGAGCAGCAA CAGGAGCCAG CCAAGATTTC TTCATTTACT 
TCACTGAAAG TTTCTCAAGA CCTCGATGTT GCAGTGATTG TCAGCTCCTC CAACTCCGCA 
GTTGCTCTTA ACTTAAATTT GTATTTCAGG CAACACCCAG GACACCTACT GTGTGAAAGA 
ATACTAGAAG ATCTTCCTAT TCAAGGACCT AAGGGCGTAG ATGAAGATGA TCCTGTTAAC 
TCTGCCTACA ACATGAAACT GGCCAAGTTT TCCTTCCAAA TTGATAGGTC TTGGAAAGCC 
CAGCTATCAT CATTGAATGA AACAATAAAG AACTCCAAAC TGGAGGTTTC CTGTTGTGCT 
CCATGGTTCC AGGATATTTT GCATTTGGAG TCACCTGAAT CTGGTAACCA CAGTACAAGT 
GTGCAGAGCT GGGCCTTCAT TCCACAGGAC ATAATGCATG GGCAATATAA TGTTCTACAG 
AAAGATCATG CCAAGACCAG TGATCCAGGA AGATCATGGA AAATAATGCA CATCAGTGAA 
CAAGAGGAAC CCATAGAGCT TAAATGTGTG TCTGTGACAG GATTCACTGC ACTGTTTACT 
TGGGAAGTGG AAAGGATGGG CTATACCATT ACCCTCTGGG ATTTGGAGAC CCAGGGCATG 
CAGTGTTTTT CCCTTGGCAC AAAGTGTATT CCTGTAGACA GTAGTGGAGA CCAGCAGCTG 
TGCTTTGTTT TGACAGAGAA TGGACTCTCT CTGATTTTGT TTGGTTTGAC TCAAGAAGAG 
TTTTTAAACA GACTCATGAT CCATGGAAGT GCCAGCACTG TGGACACTCT TTGTCATCTC 
AATGGCTGGG GAAGGTGCTC AATTCCCATA CATGCACTAG AGGCCGGGAT AGAAAATCGT 
CAGCTGGACA CAGTAAATTT CTTTTTGAAG AGCAAGGAAA ATCTTTTTAA TCCATCCTCA 
AAATCTTCTG TATCTGATCA GTTTGATCAC TTGTCATCCC ATTTATATTT AAGAAATGTG 
GAAGAGCTGA TACCAGCATT GGATTTACTT TGCTCGGCAA TTAGAGAAAG TTATTCTGAA 
CCCCAAAGCA AACACTTTTC AGAACAATTG CTTAATCTTA CACTGTCTTT CCTTAACAAC 
CAAATAAAGG AGCTTTTCAT TCACACTGAA GAACTAGATG AACATCTGCA AAAAGGAGTG 
AACATTTTGA CTAGCTACAT TAATGAACTT CGAACCTTCA TGATAAAGTT TCCTTGGAAG 
CTAACAGATG CTATAGATGA ATATGATGTA CATGAAAATG TCCCCAAAGT AAAGGAGAGC 
AATATATGGA AGAAACTCAG CTTTGAGGAA GTTATTGCCA GCGCCATTTT AAACAACAAA 
ATACCAGAGG CACAGACTTT CTTCAGGATT GATAGTCATT CTGCTCAAAA ACTTGAGGAG 
CTTATTGGCA TAGGCCTAAA TTTGGTCTTT GACAATTTAA AAAAGAACAA TATAAAGGAA 
GCCTCTGAAC TTTTGAAGAA TATGGGGTTT GATGTAAAAG GCCAATTGCT CAAGATCTGC 
TTCTATACAA CTAATAAAAA TATACGTGAC TTTTTGGTTG AAATTTTAAA AGAAAAAAAT 
TATTTTTCTG AAAAAGAGAA AAGAACTATA GACTTCGTGC ATCAAGTTGA GAAGCTTTAT 
TTGGGACATT TCCAAGAAAA TATGCAAATC CAGTCATTTC CCAGGTACTG GATAAAGGAA 
CAAGATTTTT TCAAGCACAA GTCTGTTTTG GACTCATTCC TGAAATATGA TTGTAAAGAT 
GAATTTAACA AACAGGACCA TAGAATTGTG TTAAATTGGG CTCTGTGGTG GGATCAACTA 
ACACAAGAAT CCATCCTTCT CCCCAGGATA AGTCCAGAAG AATACAAATC ATATTCCCCT 
GAAGCCCTCT GGAGATACCT CACAGCTCGC CATGATTGGT TAAACATTAT CTTATGGATT 
GGAGAATTTC AAACCCAGCA TAGTTATGCT TCACTTCAGC AGAACAAATG GCCCCTTCTG 
ACTGTTGATG TTATTAACCA GAATACTTCC TGTAACAACT ACATGAGGAA TGAAATTTTA 
GATAAGCTGG CCAGGAATGG GGTTTTTTTG GCATCTGAAC TGGAAGACTT TGAATGCTTC 
CTCCTAAGAC TGAGCCGTAT TGGAGGTGTA ATACAGGATA CCCTCCCTGT TCAAAACTAC 
AAGACCAAAG AAGGTTGGGA TTTCCATTCT CAATTCATTC TCTATTGTTT GGAGCACAGT 
CTGCAGCATC TTCTTTATGT CTACCTTGAC TGTTACAAAC TTAGTCCTGA AAATTGTCCC 
TTTTTGGAAA AAAAAGAGTT ACATGAAGCA CACCCTTGGT TTGAATTTTT AGTTCAGTGT 
CGACAAGTTG CCAGTAACTT AACAGATCCC AAACTGATCT TCCAGGCTAG CCTTGCAAAT 
GCTCAGATTT TGATTCCCAC CAATCAGGCC AGTGTAAGCA GTATGCTATT GGAAGGACAT 
ACCCTCCTGG CCCTTGCTAC TACAATGTAT TCTCCTGGGG GTGTCAGTCA GGTTGTTCAG 
AATGAAGAAA ATGAAAACTG TTTGAAGAAA GTGGATCCCC AGCTATTGAA GATGGCATTA 
ACTCCTTACC CCAAGCTAAA AACTGCTCTC TTCCCACAGT GCACTCCTCC TAGTGTCCTG 
CCATCTGATA TTACAATCTA CCACCTTATT CAGTCATTAT CACCCTTTGA TCCTAGCAGA 
TTGTTTGGCT GGCAGTCTGC TAACACACTA GCTATAGGAG ATGCATGGAG TCATCTCCCA 
CATTTCTCTA GCCCTGACCT GGTTAATAAA TATGCTATAG TGGAACGTCT GAATTTTGCT 
TATTATTTAC ATAATGGGCG GCCATCATTT GCATTTGGTA CTTTTCTGGT CCAGGAATTA 
ATCAAGAGCA AGACTCCCAA GCAGCTGATC CAGCAAGTAG GCAATGAAGC CTATGTTATA 
GGGCTCTCCT CCTTCCACAT ACCTTCAATA GGAGCTGCAT GTGTTTGTTT CTTAGAATTG 
CTTGGCCTTG ACAGCCTCAA GCTCAGAGTT GATATGAAAG TGGCCAATAT AATTTTGAGC 
TACAAGTGCA GAAATGAAGA TGCTCAGTAC AGCTTTATCA GAGAGTCTGT AGCCGAAAAA 
CTATCTAAAC TAGCTGATGG TGAAAAGACA ACCACAGAAG AATTGCTTGT TCTCTTAGAA 
GAAGGTACAT GGAACAGCAT TCAGCAACAG GAAATAAAGA GGTTATCCAG TGAATCTAGC 
AGCCAATGGG CATTAGTGGT GCAGTTCTGC AGGCTACACA ATATGAAACT AAGCATATCT 
TACCTTAGAG AATGTGCCAA AGCAAATGAT TGGCTGCAGT TCATTATTCA CAGCCAACTC 
CACAACTACC ACCCAGCAGA GGTGAAATCC CTTATCCAGT ACTTCAGCCC AGTCATTCAA 
GACCACTTAA GGCTGGCTTT TGAGAACTTG CCCTCAGTGC CCACCTCCAA AATGGACAGC 
GATCAAGTCT GCAATAAGTG CCCCCAGGAA CTTCAAGGAA GCAAACAAGA GATGACCGAT 
TTATTTGAAA TTCTGCTCCA ATGCTCAGAG GAGCCAGACT CCTGGCACTG GCTTCTGGTT 
GAAGCAGTGA AACAACAGGC CCCTATCCTC AGTGTTCTGG CCTCATGTCT CCAGGGTGCC 
AGTGCCATTT CTTGTCTCTG TGTTTGGATC ATCACTTCTG TGGAGGACAA TGTTGCAACT 
GAAGCAATGG GACACATTCA GGACTCAACA GAGGACCATA CCTGGAACCT TGAGGATCTT 
TCAGTCATCT GGAGAACATT ATTAACAAGA CAAAAGAGCA AAACTCTCAT CAGAGGTTTC 
CAGCTTTTCT TTAAGGATTC CCCGTTACTA CTGGTGATGG AGATGTATGA ACTGTGTATG 
TTCTTCAGGA ATTATAAAGA AGCTGAAGCT AAACTTCTGG AGTTTCAGAA GAGCCTTGAA 
ACGCTTAACA CAGCAGCCAC AAAGGTCCAC CCTGTCATCC CTGCCATGTG GCTGGAGGAT 
CAGGTGTGTT TCCTTTTGAA GCTTATGCTA CAGCAGTGTA AGACCCAGTA TGAGCTGGGG 
AAGCTTTTAC AGCTCTTTGT TGAAAGAGAG CATCTCTTCT CTGATGGTCC AGATGTGAAA 
AAGCTTTGCA TCCTTTGCCA GATTTTGAAG GATACATCCA TAGCCATTAA TCATACAATT 
ATTACCAGCT ACAGCATTGA GAATCTTCAG CATGAATGTA GATCTATTTT GGAAAGACTG 
CAGACAGATG GACAATTCGC TTTGGCCAGG AGGGTAGCAG AATTAGCTGA GTTACCTGTG 
GACAACTTGG TTATTAAAGA GGCCCATGTG GCATGTGAGC ACCCAACTGG ATGGAGCAGC 
ATGGAGGAGC GCCATCTGCT GCTCACCTTG GCAGGGCACT GGCTTGCCCA GGAGGACGTG 
GTGCCCTTGG ATAAGCTGGA GGAGCTGGAG AAGCAGATCT GGCTGTGCCG CATCACCCAG 
CACACTCTTG GAAGAAATCA GGAGGAAACA GAGCCCAGAT TTTCTCGACA GATCTCAACT 
AGTGGTGAAC TTTCCTTTGA TAGTTTAGCC AGTGAGTTTT CCTTCTCCAA GTTGGCTGCT 
CTGAACACAT CAAAATACTT AGAACTTAAC AGCCTTCCAT CCAAAGAGAC ATGCGAGAAT 
AGATTGGATT GGAAAGAGCA GGAGTCACTA AACTTTTTGA TTGGGCGCCT ACTGGATGAT 
GGCTGTGTGC ATGAAGCAAG TAGAGTATGC CGGTATTTTC ATTTTTATAA TCCAGATGTC 
GCCTTGGTAT TGCACTGCAG AGCACTGGCC TCAGGGGAAG CTAGTATGGA GGATCTGCAC 
CCAGAGATCC ATGCTCTCCT ACAAAGTGCT GAGCTGCTTG AGGAAGAAGC ACCCGACATT 
CCCCTAAGGA GAGTCCACAG CACTTCAAGT CTGGATAGTC AGAAGTTTGT GACAGTGCCC 
TCCAGTAATG AAGTGGTAAC TAACCTGGAA GTGCTGACAA GCAAATGCCT CCATGGGAAG 
AACTACTGTC GACAGGTCCT CTGTCTGTAT GATCTTGCCA AGGAGTTGGG CTGTTCCTAC 
ACAGATGTTG CTGCTCAGGA TGGTGAAGCC ATGCTCCGGA AAATCTTGGC CTCTCAGCAG 
CCTGACCGAT GCAAACGAGC CCAGGCCTTC ATCAGCACAC AGGGCCTTAA GCCAGATACT 
GTGGCTGAAC TCGTGGCAGA AGAGGTGACA CGGGAGCTGC TTACTTCATC ACAGGGAACA 
GGACATAAGC AGATGTTCAA CCCAACAGAG GAAAGCCAGA CATTTCTTCA GCTGACCACT 
CTGTGTCAAG ACCGCACATT GGTAGGCATG AAGTTGTTGG ATAAGATTTC CTCCGTTCCC 
CATGGGGAAC TGTCTTGCAC CACAGAGCTC CTGATCCTGG CCCATCATTG CTTCACCCTG 
ACGTGCCACA TGGAGGGCAT CATCCGAGTC CTACAGGCCG CCCACATGCT CACAGATAAC 
CACCTGGCCC CCAGTGAGGA GTATGGGCTG GTGGTACGGC TCCTCACTGG CATTGGAAGG 
TACAACGAGA TGACATACAT ATTTGATTTG CTGCATAAAA AGCACTACTT TGAAGTGCTA 
ATGAGGAAGA AGTTGGATCC GAGTGGTACC CTGAAAACAG CCCTGCTGGA CTACATCAAA 
CGCTGCCGTC CTGGAGACAG TGAAAAGCAC AATATGATTG CCCTGTGCTT CAGCATGTGC 
CGGGAGATTG GCGAGAACCA CGAGGCAGCT GCCCGCATCC AACTGAAATT GATTGAGTCT 
CAGCCCTGGG AGGACAGCCT CAAGGATGGG CACCAGCTGA AACAACTGCT GCTGAAGGCC 
CTGACTCTGA TGTTGGATGC AGCAGAGAGT TATGCCAAGG ACTCCTGTGT GCGACAGGCC 
CAGCACTGTC AGCGGCTCAC CAAGTTGATA ACTCTGCAGA TTCACTTTCT GAACACTGGC 
CAGAACACAA TGCTCATCAA CTTGGGCCGC CACAAGCTGA TGGACTGTAT TCTGGCCCTA 
CCTCGGTTCT ACCAGGCTTC TATTGTGGCT GAGGCCTACG ATTTTGTTCC AGATTGGGCT 
GAAATTTTAT ACCAGCAAGT GATTCTTAAA GGAGACTTTA ATTACTTGGA AGAATTTAAG 
CAGCAAAGGT TATTAAAGTC CAGTATATTT GAAGAGATTT CCAAAAAATA TAAACAACAT 
CAGCCTACTG ACATGGTCAT GGAAAACCTG AAGAAATTAC TCACATATTG TGAAGATGTT 
TACCTGTATT ACAAGTTGGC ATACGAACAC AAGTTTTATG AAATTGTAAA TGTGCTTCTG 
AAGGACCCTC AGACAGGTTG CTGTCTAAAG GACATGCTAG CAGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SPG11
Entrez Gene ID	80208
Full Name	SPG11, spatacsin vesicle trafficking associated
Synonyms	ALS5,CMT2X,KIAA1840
General protein information	Preferred Names SPG11, spatacsin vesicle trafficking associated Names spatacsin colorectal carcinoma-associated protein spastic paraplegia 11 (autosomal recessive) spastic paraplegia 11 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q21.1
Summary	The protein encoded by this gene is a potential transmembrane protein that is phosphorylated upon DNA damage. Defects in this gene are a cause of spastic paraplegia type 11 (SPG11). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009].
Disorder MIM:	610844
Disorder Html:	Spastic paraplegia 11, autosomal recessive, 604360 (3); Amyotrophic lateral sclerosis 5, juvenile, 602099 (3); Charcot-Marie-Tooth disease, axonal, type 2X, 616668 (3)

Rattus norvegicus (Norway rat)	Spg11	NP_001178500.1
Homo sapiens (human)	SPG11	NP_079413.3
Canis lupus familiaris (dog)	SPG11	XP_003640073.2
Bos taurus (cattle)	SPG11	XP_002691138.2
Mus musculus (house mouse)	Spg11	NP_663506.2
	LOC712221	XP_002808495.1
Gallus gallus (chicken)	SPG11	XP_003641879.2
Danio rerio (zebrafish)	spg11	NP_001181923.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SPG11 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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