ARHGEF12 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARHGEF12
Entrez Gene ID	451618
Full Name	Rho guanine nucleotide exchange factor 12
General protein information	Preferred Names Rho guanine nucleotide exchange factor 12 Names rho guanine nucleotide exchange factor 12 Rho guanine nucleotide exchange factor (GEF) 12
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016922155.1	XP_016777644.1	rho guanine nucleotide exchange factor 12 isoform X1
XM_016922159.2	XP_016777648.1	rho guanine nucleotide exchange factor 12 isoform X4
XM_016922155.2	XP_016777644.1	rho guanine nucleotide exchange factor 12 isoform X1
XM_016922158.2	XP_016777647.1	rho guanine nucleotide exchange factor 12 isoform X3
XM_016922158.1	XP_016777647.1	rho guanine nucleotide exchange factor 12 isoform X3
XM_016922157.1	XP_016777646.1	rho guanine nucleotide exchange factor 12 isoform X2
XM_016922159.1	XP_016777648.1	rho guanine nucleotide exchange factor 12 isoform X4
XM_016922157.2	XP_016777646.1	rho guanine nucleotide exchange factor 12 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ARHGEF12	XP_005619719.1
	ARHGEF12	XP_002799913.1
Gallus gallus (chicken)	ARHGEF12	XP_417890.4
Danio rerio (zebrafish)	arhgef12	XP_005172066.1
Pan troglodytes (chimpanzee)	ARHGEF12	XP_001166830.1
Xenopus tropicalis (tropical clawed frog)	arhgef12	NP_001072496.1
Homo sapiens (human)	ARHGEF12	NP_056128.1
Bos taurus (cattle)	ARHGEF12	NP_001116505.1
Mus musculus (house mouse)	Arhgef12	NP_081420.2
Rattus norvegicus (Norway rat)	Arhgef12	NP_001013264.1
Danio rerio (zebrafish)	LOC562455	XP_003200092.2

Gene Ontology

The following ARHGEF12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGEF12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc64928	XM_016922159.2 Latest version!	Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc64925	XM_016922155.2 Latest version!	Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc64926	XM_016922157.2 Latest version!	Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc64927	XM_016922158.2 Latest version!	Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
View Old Accession Versions >>
OPc64925	XM_016922155.1	Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc64927	XM_016922158.1	Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc64928	XM_016922159.1	Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc64926	XM_016922157.1	Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARHGEF12 ( XM_016922159.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016777648.1 Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPc64928
Clone ID Related Accession (Same CDS sequence)	XM_016922159.2 , XM_016922159.1
Accession Version	XM_016922159.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	rho guanine nucleotide exchange factor 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016922159.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
CACTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG 
TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ACTCACCTGG AGCATCTGGG 
AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTCAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGTTATTG 
CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG 
AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA 
GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA 
GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGTTTC TCGGCCCAGT 
AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC 
CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA 
ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA 
CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT 
CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT 
TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT 
GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA 
ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT 
CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA 
GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC 
ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA 
AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG 
AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC 
CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG 
GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT 
GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC 
AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA 
CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG 
GAGGAATGTG AAGTAGAAAG GGTGACAGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG 
ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT 
TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG 
TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT 
GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG 
ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAGTC TCGTCAGAAA 
AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT 
CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG 
TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CCGTCAAGGA GGCAGAAAAC 
AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA 
GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC ATTGCTGCTG 
GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT 
AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT 
ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA 
GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA 
TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA 
GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA 
GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG 
ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC 
TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG 
GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA 
TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT 
AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA 
ACGTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG 
GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA 
GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCTATGACA 
GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC 
GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG 
GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC 
ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016777648.1
CDS	2069..6394
Translation	MRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDVSRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_016922159.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016922159.2

ORF Insert Sequence:

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
CACTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG 
TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ACTCACCTGG AGCATCTGGG 
AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTCAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGTTATTG 
CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG 
AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA 
GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA 
GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGTTTC TCGGCCCAGT 
AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC 
CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA 
ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA 
CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT 
CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT 
TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT 
GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA 
ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT 
CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA 
GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC 
ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA 
AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG 
AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC 
CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG 
GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT 
GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC 
AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA 
CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG 
GAGGAATGTG AAGTAGAAAG GGTGACAGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG 
ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT 
TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG 
TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT 
GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG 
ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAGTC TCGTCAGAAA 
AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT 
CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG 
TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CCGTCAAGGA GGCAGAAAAC 
AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA 
GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC ATTGCTGCTG 
GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT 
AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT 
ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA 
GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA 
TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA 
GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA 
GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG 
ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC 
TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG 
GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA 
TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT 
AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA 
ACGTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG 
GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA 
GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCTATGACA 
GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC 
GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG 
GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC 
ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_016922159.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016777648.1 Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPc64928
Clone ID Related Accession (Same CDS sequence)	XM_016922159.2 , XM_016922159.1
Accession Version	XM_016922159.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	rho guanine nucleotide exchange factor 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006478.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
CACTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG 
TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ACTCACCTGG AGCATCTGGG 
AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTCAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGTTATTG 
CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG 
AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA 
GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA 
GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGTTTC TCGGCCCAGT 
AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC 
CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA 
ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA 
CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT 
CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT 
TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT 
GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA 
ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT 
CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA 
GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC 
ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA 
AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG 
AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC 
CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG 
GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT 
GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC 
AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA 
CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG 
GAGGAATGTG AAGTAGAAAG GGTGACAGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG 
ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT 
TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG 
TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT 
GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG 
ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAGTC TCGTCAGAAA 
AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT 
CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG 
TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CCGTCAAGGA GGCAGAAAAC 
AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA 
GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC ATTGCTGCTG 
GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT 
AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT 
ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA 
GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA 
TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA 
GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA 
GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG 
ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC 
TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG 
GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA 
TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT 
AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA 
ACGTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG 
GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA 
GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCTATGACA 
GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC 
GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG 
GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC 
ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016777648.1
CDS	2058..6383
Translation	MRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDVSRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_016922159.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016922159.1

ORF Insert Sequence:

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
CACTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG 
TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ACTCACCTGG AGCATCTGGG 
AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTCAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGTTATTG 
CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG 
AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA 
GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA 
GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGTTTC TCGGCCCAGT 
AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC 
CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA 
ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA 
CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT 
CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT 
TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT 
GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA 
ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT 
CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA 
GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC 
ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA 
AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG 
AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC 
CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG 
GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT 
GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC 
AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA 
CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG 
GAGGAATGTG AAGTAGAAAG GGTGACAGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG 
ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT 
TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG 
TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT 
GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG 
ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAGTC TCGTCAGAAA 
AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT 
CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG 
TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CCGTCAAGGA GGCAGAAAAC 
AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA 
GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC ATTGCTGCTG 
GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT 
AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT 
ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA 
GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA 
TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA 
GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA 
GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG 
ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC 
TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG 
GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA 
TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT 
AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA 
ACGTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG 
GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA 
GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCTATGACA 
GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC 
GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG 
GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC 
ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_016922155.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016777644.1 Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA.

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CloneID	OPc64925
Clone ID Related Accession (Same CDS sequence)	XM_016922155.1 , XM_016922155.2
Accession Version	XM_016922155.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	rho guanine nucleotide exchange factor 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006478.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGCGTAA TCATCCAGAA AGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ACTCAAATCA TCTGGAGGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGACTCTGAA 
GTAGAGCCGT CAGTCATTGG ACATATGTCT CCCATCATGA CATCTCCTCA CTCACCTGGA 
GCATCTGGGA ATATGGAGAG AATCACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTCAGA AAAATGTTAC AGAAAGAACA GGAACGGCTA 
CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC ACCCTCCAGA CCTTTAGGGG ACACCCTAAC AGTCAGTGAG 
GCAGAAACAG ATCCTGGAGA TGTACTGGGC AGGACTGACT GTAGCAGTGG AGATGTTTCT 
CGGCCCAGTA GTGACAATGC AGATAGTCCC AAGAGTGGCC CAAAAGAGAG AATTTATCTA 
GAGGAAAACC CAGAGAAAAG TGAAACAATT CAGGACACTG ACACTCAATC ACTTGTCGGA 
AGTCCCTCAA CCCGTATAGC ACCTCATATT ATTGGAGCAG AAGATGATGA TTTTGGTACT 
GAACATGAAC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT ACTAAAATCT 
CGCCCGGCTC ATTTGGCTGT TTTCTTACAC CATGTAGTTT CACAATTTGA CCCTGCGACT 
TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAACATACCA ATTCCAAAGA AACTCGTCGC 
ATCTTCCTTG AGTTTCATCA GTTCTTTCTA GATCGATCAG CACACCTGAA AGTTTCTGTT 
CCTGATGAAA TGTCTGCAGA TCTAGAAAAG AGAAGACCTG AGCTCATTCC TGAGGATCTG 
CATCGCCACT ATATCCAAAC TATGCAAGAA AGAGTCCATC CAGAAGTTCA AAGGCACTTA 
GAAGATTTTC GGCAGAAACG TAGTATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA 
CTTGATGCAG AGCGAGACAA GGACCGATTG ACTTTGGAGA AGGAGCGGAC ATGTGCAGAA 
CAGATTGTTG CCAAAATTGA AGAAGTATTG ATGACTGCTC AGGCTGTAGA GGAAGATAAG 
AGCTCCACCA TGCAGTATGT TATTCTCATG TATATGAAGC ATTTGGGAGT AAAAGTGAAA 
GAGCCTCGAA ATTTGGAGCA CAAACGGGGT CGGATTGGAT TTCTTCCCAA AATCAAGCAA 
AGTATGAAGA AAGATAAAGA AGGGGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATC 
CTGGGACCCC CACGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCCATGGAA 
CTACAGAAGG CGCGCCACCC TAAGCACTTA TCCACACCCT CATCTGTGAG TCCTGAACCT 
CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAAATG AAGGAACAGA CGCTGGATAC 
CTGCCTGCCA ATTCCATGTC TTCTGTAGCT TCAGGGGCCT CTTTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAT CAGCACCTGG AGACACCTTA 
GATGGCACAC CTCGTACTCT CAATACTGTC TTTGATTTCC CACCACCTCC ATTAGACCAA 
GTGCAGGAGG AGGAATGTGA AGTAGAAAGG GTGACAGAAC ATGGGACACC AAAGCCCTTT 
CGAAAGTTTG ACAGTGTAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA ATTTGAAAAT 
GACTTAGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GTCGAGAAGT GTTACTGGGA 
CTAAAACCTT GTGAAATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ATCAGCGAGT ATCCAGAGAA 
GGAATTCTGT CACCCTCAGA GCTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA 
CTTCATATTG GATTGAATGA ACAAATGAAG GCTGTTCGAA AGAGAAATGA GACCTCTGTT 
ATCGATCAGA TTGGGGAAGA TTTGCTGACA TGGTTCAGCG GACCAGGAGA GGAGAAATTG 
AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAGTCT 
CGTCAGAAAA AGGATTCTCG ATTTCAGACT TTTGTGCAAG ATGCTGAAAG TAATCCACTG 
TGTCGTCGTC TTCAACTGAA GGATATTATT CCCACTCAAA TGCAAAGGCT TACTAAGTAC 
CCACTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAACAGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTATG TAAATCAGGC CGTCAAGGAG 
GCAGAAAACA AGCAGCGCCT AGAAGATTAT CAGCGTCGCC TTGATACCTC CAGCCTGAAG 
TTGTCAGAGT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCATGAAG GGCCATTGGT TTGGAAGGTG AATAGAGATA AAACTATTGA TTTATACACA 
TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTTTTAAGG 
TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT 
AAGTTGAGTA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATT 
TCCATGTCAG ACAATGGCGC TCAGATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAGCA ATCCACAAAG 
CCAATTCCAT TACCACAGTC AACACCTGGC GAAGGAGATA ATGATGAAGA AGATCCTTCA 
AAATTAAAAG AGGAGCAGCA TGGCATTTCA GTCACTGGTT TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AATCTACCTT AATATCGTCA AAACCTCAGT CTCATTCACT GAGTACCTCT 
GGGAAATCAG AGGTACGTGA TCTGTTTGTG GCTGAGAGAC AGTTTGCAAA GGAACAACAT 
ACAGATGGGA CACTAAAGGA AGTTGGAGAA GATTATCAAA TCGCAATCCC AGATTCACAC 
CTGCCTGTCT CAGAAGAACG GTGGGCATTG GATGCACTAA GAAATTTGGG TTTGTTGAAG 
CAGTTGCTGG TGCAACAGCT AGGTTTGACT GAGAAGAGCG TTCAGGAAGA CTGGCAACAT 
TTCCCAAGAT ACAGAACAGC CTCTCAGGGG CCGCAGACAG ACAGTGTCAT CCAGAACTCT 
GAAAATATTA AGGCCTATCA TTCTGGTGAA GGACATATGC CCTTTAGAAC TGGAACTGGT 
GACATTGCAA CGTGTTACAG TCCACGGACT TCAACTGAAT CTTTTGCTCC ACGGGATTCA 
GTGGGACTGG CACCCCAGGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTAC CATGGAGCCA GAAGGGGGTC TTGATGACAG TGGAGAGCAC 
TTTTTTGATG CCCGTGAAGC ACATAGTGAT GAGAATCCAT CAGAAGGTGA TGGAGCAGTT 
AACAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTATTTGAT CCTTGATGGC 
TATGACCCAG TGCAGGAGAG TTCCACAGAT GAGGAGGTTG CTTCCTCACT TACCCTGCAG 
CCTATGACAG GCATCCCTGC TGTGGAATCC ACCCACCAGC AGCAACATTC TCCTCAGAAT 
ACTCACTCCG ATGGGGCAAT TTCACCATTC ACCCCCGAAT TTCTGGTCCA GCAGCGCTGG 
GGAGCTATGG AGTATTCCTG TTTTGAGATC CAGAGTCCCT CCTCTTGTGC AGATTCACAG 
AGCCAGATCA TGGAGTACAT TCATAAGATA GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016777644.1
CDS	336..4970
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERIASHDFDPTDSSSKKTKSSSEESRSEIYGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDVSRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_016922155.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016922155.1

ORF Insert Sequence:

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGCGTAA TCATCCAGAA AGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ACTCAAATCA TCTGGAGGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGACTCTGAA 
GTAGAGCCGT CAGTCATTGG ACATATGTCT CCCATCATGA CATCTCCTCA CTCACCTGGA 
GCATCTGGGA ATATGGAGAG AATCACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTCAGA AAAATGTTAC AGAAAGAACA GGAACGGCTA 
CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC ACCCTCCAGA CCTTTAGGGG ACACCCTAAC AGTCAGTGAG 
GCAGAAACAG ATCCTGGAGA TGTACTGGGC AGGACTGACT GTAGCAGTGG AGATGTTTCT 
CGGCCCAGTA GTGACAATGC AGATAGTCCC AAGAGTGGCC CAAAAGAGAG AATTTATCTA 
GAGGAAAACC CAGAGAAAAG TGAAACAATT CAGGACACTG ACACTCAATC ACTTGTCGGA 
AGTCCCTCAA CCCGTATAGC ACCTCATATT ATTGGAGCAG AAGATGATGA TTTTGGTACT 
GAACATGAAC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT ACTAAAATCT 
CGCCCGGCTC ATTTGGCTGT TTTCTTACAC CATGTAGTTT CACAATTTGA CCCTGCGACT 
TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAACATACCA ATTCCAAAGA AACTCGTCGC 
ATCTTCCTTG AGTTTCATCA GTTCTTTCTA GATCGATCAG CACACCTGAA AGTTTCTGTT 
CCTGATGAAA TGTCTGCAGA TCTAGAAAAG AGAAGACCTG AGCTCATTCC TGAGGATCTG 
CATCGCCACT ATATCCAAAC TATGCAAGAA AGAGTCCATC CAGAAGTTCA AAGGCACTTA 
GAAGATTTTC GGCAGAAACG TAGTATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA 
CTTGATGCAG AGCGAGACAA GGACCGATTG ACTTTGGAGA AGGAGCGGAC ATGTGCAGAA 
CAGATTGTTG CCAAAATTGA AGAAGTATTG ATGACTGCTC AGGCTGTAGA GGAAGATAAG 
AGCTCCACCA TGCAGTATGT TATTCTCATG TATATGAAGC ATTTGGGAGT AAAAGTGAAA 
GAGCCTCGAA ATTTGGAGCA CAAACGGGGT CGGATTGGAT TTCTTCCCAA AATCAAGCAA 
AGTATGAAGA AAGATAAAGA AGGGGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATC 
CTGGGACCCC CACGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCCATGGAA 
CTACAGAAGG CGCGCCACCC TAAGCACTTA TCCACACCCT CATCTGTGAG TCCTGAACCT 
CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAAATG AAGGAACAGA CGCTGGATAC 
CTGCCTGCCA ATTCCATGTC TTCTGTAGCT TCAGGGGCCT CTTTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAT CAGCACCTGG AGACACCTTA 
GATGGCACAC CTCGTACTCT CAATACTGTC TTTGATTTCC CACCACCTCC ATTAGACCAA 
GTGCAGGAGG AGGAATGTGA AGTAGAAAGG GTGACAGAAC ATGGGACACC AAAGCCCTTT 
CGAAAGTTTG ACAGTGTAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA ATTTGAAAAT 
GACTTAGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GTCGAGAAGT GTTACTGGGA 
CTAAAACCTT GTGAAATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ATCAGCGAGT ATCCAGAGAA 
GGAATTCTGT CACCCTCAGA GCTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA 
CTTCATATTG GATTGAATGA ACAAATGAAG GCTGTTCGAA AGAGAAATGA GACCTCTGTT 
ATCGATCAGA TTGGGGAAGA TTTGCTGACA TGGTTCAGCG GACCAGGAGA GGAGAAATTG 
AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAGTCT 
CGTCAGAAAA AGGATTCTCG ATTTCAGACT TTTGTGCAAG ATGCTGAAAG TAATCCACTG 
TGTCGTCGTC TTCAACTGAA GGATATTATT CCCACTCAAA TGCAAAGGCT TACTAAGTAC 
CCACTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAACAGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTATG TAAATCAGGC CGTCAAGGAG 
GCAGAAAACA AGCAGCGCCT AGAAGATTAT CAGCGTCGCC TTGATACCTC CAGCCTGAAG 
TTGTCAGAGT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCATGAAG GGCCATTGGT TTGGAAGGTG AATAGAGATA AAACTATTGA TTTATACACA 
TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTTTTAAGG 
TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT 
AAGTTGAGTA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATT 
TCCATGTCAG ACAATGGCGC TCAGATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAGCA ATCCACAAAG 
CCAATTCCAT TACCACAGTC AACACCTGGC GAAGGAGATA ATGATGAAGA AGATCCTTCA 
AAATTAAAAG AGGAGCAGCA TGGCATTTCA GTCACTGGTT TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AATCTACCTT AATATCGTCA AAACCTCAGT CTCATTCACT GAGTACCTCT 
GGGAAATCAG AGGTACGTGA TCTGTTTGTG GCTGAGAGAC AGTTTGCAAA GGAACAACAT 
ACAGATGGGA CACTAAAGGA AGTTGGAGAA GATTATCAAA TCGCAATCCC AGATTCACAC 
CTGCCTGTCT CAGAAGAACG GTGGGCATTG GATGCACTAA GAAATTTGGG TTTGTTGAAG 
CAGTTGCTGG TGCAACAGCT AGGTTTGACT GAGAAGAGCG TTCAGGAAGA CTGGCAACAT 
TTCCCAAGAT ACAGAACAGC CTCTCAGGGG CCGCAGACAG ACAGTGTCAT CCAGAACTCT 
GAAAATATTA AGGCCTATCA TTCTGGTGAA GGACATATGC CCTTTAGAAC TGGAACTGGT 
GACATTGCAA CGTGTTACAG TCCACGGACT TCAACTGAAT CTTTTGCTCC ACGGGATTCA 
GTGGGACTGG CACCCCAGGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTAC CATGGAGCCA GAAGGGGGTC TTGATGACAG TGGAGAGCAC 
TTTTTTGATG CCCGTGAAGC ACATAGTGAT GAGAATCCAT CAGAAGGTGA TGGAGCAGTT 
AACAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTATTTGAT CCTTGATGGC 
TATGACCCAG TGCAGGAGAG TTCCACAGAT GAGGAGGTTG CTTCCTCACT TACCCTGCAG 
CCTATGACAG GCATCCCTGC TGTGGAATCC ACCCACCAGC AGCAACATTC TCCTCAGAAT 
ACTCACTCCG ATGGGGCAAT TTCACCATTC ACCCCCGAAT TTCTGGTCCA GCAGCGCTGG 
GGAGCTATGG AGTATTCCTG TTTTGAGATC CAGAGTCCCT CCTCTTGTGC AGATTCACAG 
AGCCAGATCA TGGAGTACAT TCATAAGATA GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_016922155.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016777644.1 Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA.

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CloneID	OPc64925
Clone ID Related Accession (Same CDS sequence)	XM_016922155.1 , XM_016922155.2
Accession Version	XM_016922155.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	rho guanine nucleotide exchange factor 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016922155.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGCGTAA TCATCCAGAA AGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ACTCAAATCA TCTGGAGGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGACTCTGAA 
GTAGAGCCGT CAGTCATTGG ACATATGTCT CCCATCATGA CATCTCCTCA CTCACCTGGA 
GCATCTGGGA ATATGGAGAG AATCACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTCAGA AAAATGTTAC AGAAAGAACA GGAACGGCTA 
CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC ACCCTCCAGA CCTTTAGGGG ACACCCTAAC AGTCAGTGAG 
GCAGAAACAG ATCCTGGAGA TGTACTGGGC AGGACTGACT GTAGCAGTGG AGATGTTTCT 
CGGCCCAGTA GTGACAATGC AGATAGTCCC AAGAGTGGCC CAAAAGAGAG AATTTATCTA 
GAGGAAAACC CAGAGAAAAG TGAAACAATT CAGGACACTG ACACTCAATC ACTTGTCGGA 
AGTCCCTCAA CCCGTATAGC ACCTCATATT ATTGGAGCAG AAGATGATGA TTTTGGTACT 
GAACATGAAC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT ACTAAAATCT 
CGCCCGGCTC ATTTGGCTGT TTTCTTACAC CATGTAGTTT CACAATTTGA CCCTGCGACT 
TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAACATACCA ATTCCAAAGA AACTCGTCGC 
ATCTTCCTTG AGTTTCATCA GTTCTTTCTA GATCGATCAG CACACCTGAA AGTTTCTGTT 
CCTGATGAAA TGTCTGCAGA TCTAGAAAAG AGAAGACCTG AGCTCATTCC TGAGGATCTG 
CATCGCCACT ATATCCAAAC TATGCAAGAA AGAGTCCATC CAGAAGTTCA AAGGCACTTA 
GAAGATTTTC GGCAGAAACG TAGTATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA 
CTTGATGCAG AGCGAGACAA GGACCGATTG ACTTTGGAGA AGGAGCGGAC ATGTGCAGAA 
CAGATTGTTG CCAAAATTGA AGAAGTATTG ATGACTGCTC AGGCTGTAGA GGAAGATAAG 
AGCTCCACCA TGCAGTATGT TATTCTCATG TATATGAAGC ATTTGGGAGT AAAAGTGAAA 
GAGCCTCGAA ATTTGGAGCA CAAACGGGGT CGGATTGGAT TTCTTCCCAA AATCAAGCAA 
AGTATGAAGA AAGATAAAGA AGGGGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATC 
CTGGGACCCC CACGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCCATGGAA 
CTACAGAAGG CGCGCCACCC TAAGCACTTA TCCACACCCT CATCTGTGAG TCCTGAACCT 
CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAAATG AAGGAACAGA CGCTGGATAC 
CTGCCTGCCA ATTCCATGTC TTCTGTAGCT TCAGGGGCCT CTTTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAT CAGCACCTGG AGACACCTTA 
GATGGCACAC CTCGTACTCT CAATACTGTC TTTGATTTCC CACCACCTCC ATTAGACCAA 
GTGCAGGAGG AGGAATGTGA AGTAGAAAGG GTGACAGAAC ATGGGACACC AAAGCCCTTT 
CGAAAGTTTG ACAGTGTAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA ATTTGAAAAT 
GACTTAGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GTCGAGAAGT GTTACTGGGA 
CTAAAACCTT GTGAAATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ATCAGCGAGT ATCCAGAGAA 
GGAATTCTGT CACCCTCAGA GCTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA 
CTTCATATTG GATTGAATGA ACAAATGAAG GCTGTTCGAA AGAGAAATGA GACCTCTGTT 
ATCGATCAGA TTGGGGAAGA TTTGCTGACA TGGTTCAGCG GACCAGGAGA GGAGAAATTG 
AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAGTCT 
CGTCAGAAAA AGGATTCTCG ATTTCAGACT TTTGTGCAAG ATGCTGAAAG TAATCCACTG 
TGTCGTCGTC TTCAACTGAA GGATATTATT CCCACTCAAA TGCAAAGGCT TACTAAGTAC 
CCACTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAACAGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTATG TAAATCAGGC CGTCAAGGAG 
GCAGAAAACA AGCAGCGCCT AGAAGATTAT CAGCGTCGCC TTGATACCTC CAGCCTGAAG 
TTGTCAGAGT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCATGAAG GGCCATTGGT TTGGAAGGTG AATAGAGATA AAACTATTGA TTTATACACA 
TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTTTTAAGG 
TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT 
AAGTTGAGTA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATT 
TCCATGTCAG ACAATGGCGC TCAGATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAGCA ATCCACAAAG 
CCAATTCCAT TACCACAGTC AACACCTGGC GAAGGAGATA ATGATGAAGA AGATCCTTCA 
AAATTAAAAG AGGAGCAGCA TGGCATTTCA GTCACTGGTT TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AATCTACCTT AATATCGTCA AAACCTCAGT CTCATTCACT GAGTACCTCT 
GGGAAATCAG AGGTACGTGA TCTGTTTGTG GCTGAGAGAC AGTTTGCAAA GGAACAACAT 
ACAGATGGGA CACTAAAGGA AGTTGGAGAA GATTATCAAA TCGCAATCCC AGATTCACAC 
CTGCCTGTCT CAGAAGAACG GTGGGCATTG GATGCACTAA GAAATTTGGG TTTGTTGAAG 
CAGTTGCTGG TGCAACAGCT AGGTTTGACT GAGAAGAGCG TTCAGGAAGA CTGGCAACAT 
TTCCCAAGAT ACAGAACAGC CTCTCAGGGG CCGCAGACAG ACAGTGTCAT CCAGAACTCT 
GAAAATATTA AGGCCTATCA TTCTGGTGAA GGACATATGC CCTTTAGAAC TGGAACTGGT 
GACATTGCAA CGTGTTACAG TCCACGGACT TCAACTGAAT CTTTTGCTCC ACGGGATTCA 
GTGGGACTGG CACCCCAGGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTAC CATGGAGCCA GAAGGGGGTC TTGATGACAG TGGAGAGCAC 
TTTTTTGATG CCCGTGAAGC ACATAGTGAT GAGAATCCAT CAGAAGGTGA TGGAGCAGTT 
AACAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTATTTGAT CCTTGATGGC 
TATGACCCAG TGCAGGAGAG TTCCACAGAT GAGGAGGTTG CTTCCTCACT TACCCTGCAG 
CCTATGACAG GCATCCCTGC TGTGGAATCC ACCCACCAGC AGCAACATTC TCCTCAGAAT 
ACTCACTCCG ATGGGGCAAT TTCACCATTC ACCCCCGAAT TTCTGGTCCA GCAGCGCTGG 
GGAGCTATGG AGTATTCCTG TTTTGAGATC CAGAGTCCCT CCTCTTGTGC AGATTCACAG 
AGCCAGATCA TGGAGTACAT TCATAAGATA GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016777644.1
CDS	336..4970
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERIASHDFDPTDSSSKKTKSSSEESRSEIYGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDVSRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_016922155.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016922155.2

ORF Insert Sequence:

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGCGTAA TCATCCAGAA AGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ACTCAAATCA TCTGGAGGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGACTCTGAA 
GTAGAGCCGT CAGTCATTGG ACATATGTCT CCCATCATGA CATCTCCTCA CTCACCTGGA 
GCATCTGGGA ATATGGAGAG AATCACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTCAGA AAAATGTTAC AGAAAGAACA GGAACGGCTA 
CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC ACCCTCCAGA CCTTTAGGGG ACACCCTAAC AGTCAGTGAG 
GCAGAAACAG ATCCTGGAGA TGTACTGGGC AGGACTGACT GTAGCAGTGG AGATGTTTCT 
CGGCCCAGTA GTGACAATGC AGATAGTCCC AAGAGTGGCC CAAAAGAGAG AATTTATCTA 
GAGGAAAACC CAGAGAAAAG TGAAACAATT CAGGACACTG ACACTCAATC ACTTGTCGGA 
AGTCCCTCAA CCCGTATAGC ACCTCATATT ATTGGAGCAG AAGATGATGA TTTTGGTACT 
GAACATGAAC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT ACTAAAATCT 
CGCCCGGCTC ATTTGGCTGT TTTCTTACAC CATGTAGTTT CACAATTTGA CCCTGCGACT 
TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAACATACCA ATTCCAAAGA AACTCGTCGC 
ATCTTCCTTG AGTTTCATCA GTTCTTTCTA GATCGATCAG CACACCTGAA AGTTTCTGTT 
CCTGATGAAA TGTCTGCAGA TCTAGAAAAG AGAAGACCTG AGCTCATTCC TGAGGATCTG 
CATCGCCACT ATATCCAAAC TATGCAAGAA AGAGTCCATC CAGAAGTTCA AAGGCACTTA 
GAAGATTTTC GGCAGAAACG TAGTATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA 
CTTGATGCAG AGCGAGACAA GGACCGATTG ACTTTGGAGA AGGAGCGGAC ATGTGCAGAA 
CAGATTGTTG CCAAAATTGA AGAAGTATTG ATGACTGCTC AGGCTGTAGA GGAAGATAAG 
AGCTCCACCA TGCAGTATGT TATTCTCATG TATATGAAGC ATTTGGGAGT AAAAGTGAAA 
GAGCCTCGAA ATTTGGAGCA CAAACGGGGT CGGATTGGAT TTCTTCCCAA AATCAAGCAA 
AGTATGAAGA AAGATAAAGA AGGGGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATC 
CTGGGACCCC CACGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCCATGGAA 
CTACAGAAGG CGCGCCACCC TAAGCACTTA TCCACACCCT CATCTGTGAG TCCTGAACCT 
CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAAATG AAGGAACAGA CGCTGGATAC 
CTGCCTGCCA ATTCCATGTC TTCTGTAGCT TCAGGGGCCT CTTTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAT CAGCACCTGG AGACACCTTA 
GATGGCACAC CTCGTACTCT CAATACTGTC TTTGATTTCC CACCACCTCC ATTAGACCAA 
GTGCAGGAGG AGGAATGTGA AGTAGAAAGG GTGACAGAAC ATGGGACACC AAAGCCCTTT 
CGAAAGTTTG ACAGTGTAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA ATTTGAAAAT 
GACTTAGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GTCGAGAAGT GTTACTGGGA 
CTAAAACCTT GTGAAATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ATCAGCGAGT ATCCAGAGAA 
GGAATTCTGT CACCCTCAGA GCTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA 
CTTCATATTG GATTGAATGA ACAAATGAAG GCTGTTCGAA AGAGAAATGA GACCTCTGTT 
ATCGATCAGA TTGGGGAAGA TTTGCTGACA TGGTTCAGCG GACCAGGAGA GGAGAAATTG 
AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAGTCT 
CGTCAGAAAA AGGATTCTCG ATTTCAGACT TTTGTGCAAG ATGCTGAAAG TAATCCACTG 
TGTCGTCGTC TTCAACTGAA GGATATTATT CCCACTCAAA TGCAAAGGCT TACTAAGTAC 
CCACTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAACAGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTATG TAAATCAGGC CGTCAAGGAG 
GCAGAAAACA AGCAGCGCCT AGAAGATTAT CAGCGTCGCC TTGATACCTC CAGCCTGAAG 
TTGTCAGAGT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCATGAAG GGCCATTGGT TTGGAAGGTG AATAGAGATA AAACTATTGA TTTATACACA 
TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTTTTAAGG 
TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT 
AAGTTGAGTA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATT 
TCCATGTCAG ACAATGGCGC TCAGATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAGCA ATCCACAAAG 
CCAATTCCAT TACCACAGTC AACACCTGGC GAAGGAGATA ATGATGAAGA AGATCCTTCA 
AAATTAAAAG AGGAGCAGCA TGGCATTTCA GTCACTGGTT TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AATCTACCTT AATATCGTCA AAACCTCAGT CTCATTCACT GAGTACCTCT 
GGGAAATCAG AGGTACGTGA TCTGTTTGTG GCTGAGAGAC AGTTTGCAAA GGAACAACAT 
ACAGATGGGA CACTAAAGGA AGTTGGAGAA GATTATCAAA TCGCAATCCC AGATTCACAC 
CTGCCTGTCT CAGAAGAACG GTGGGCATTG GATGCACTAA GAAATTTGGG TTTGTTGAAG 
CAGTTGCTGG TGCAACAGCT AGGTTTGACT GAGAAGAGCG TTCAGGAAGA CTGGCAACAT 
TTCCCAAGAT ACAGAACAGC CTCTCAGGGG CCGCAGACAG ACAGTGTCAT CCAGAACTCT 
GAAAATATTA AGGCCTATCA TTCTGGTGAA GGACATATGC CCTTTAGAAC TGGAACTGGT 
GACATTGCAA CGTGTTACAG TCCACGGACT TCAACTGAAT CTTTTGCTCC ACGGGATTCA 
GTGGGACTGG CACCCCAGGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTAC CATGGAGCCA GAAGGGGGTC TTGATGACAG TGGAGAGCAC 
TTTTTTGATG CCCGTGAAGC ACATAGTGAT GAGAATCCAT CAGAAGGTGA TGGAGCAGTT 
AACAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTATTTGAT CCTTGATGGC 
TATGACCCAG TGCAGGAGAG TTCCACAGAT GAGGAGGTTG CTTCCTCACT TACCCTGCAG 
CCTATGACAG GCATCCCTGC TGTGGAATCC ACCCACCAGC AGCAACATTC TCCTCAGAAT 
ACTCACTCCG ATGGGGCAAT TTCACCATTC ACCCCCGAAT TTCTGGTCCA GCAGCGCTGG 
GGAGCTATGG AGTATTCCTG TTTTGAGATC CAGAGTCCCT CCTCTTGTGC AGATTCACAG 
AGCCAGATCA TGGAGTACAT TCATAAGATA GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_016922157.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016777646.1 Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA.

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CloneID	OPc64926
Clone ID Related Accession (Same CDS sequence)	XM_016922157.1 , XM_016922157.2
Accession Version	XM_016922157.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	rho guanine nucleotide exchange factor 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006478.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGGTCTTGTT CAGCGTTGCG TAATCATCCA GAAAGATGAC 
AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA 
GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA 
ACTCTGGTGA CTCACTCAAA TCATCTGGAG GTGGTGAAGC TAATCAAATC TGGTTCCTAT 
GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT TGCCGACTCT 
GAAGTAGAGC CGTCAGTCAT TGGACATATG TCTCCCATCA TGACATCTCC TCACTCACCT 
GGAGCATCTG GGAATATGGA GAGAATCACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT 
GTGGTTCATA ACCAGAAAGT AGAAATTCTC AGAAAAATGT TACAGAAAGA ACAGGAACGG 
CTACAGTTAT TGCAGGAAGA TTACAACCGA ACACCTGCCC AAAGATTGCT AAAAGAGATC 
CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT 
GCTCAGGATG GAGCTGTAGT TACACCCTCC AGACCTTTAG GGGACACCCT AACAGTCAGT 
GAGGCAGAAA CAGATCCTGG AGATGTACTG GGCAGGACTG ACTGTAGCAG TGGAGATGTT 
TCTCGGCCCA GTAGTGACAA TGCAGATAGT CCCAAGAGTG GCCCAAAAGA GAGAATTTAT 
CTAGAGGAAA ACCCAGAGAA AAGTGAAACA ATTCAGGACA CTGACACTCA ATCACTTGTC 
GGAAGTCCCT CAACCCGTAT AGCACCTCAT ATTATTGGAG CAGAAGATGA TGATTTTGGT 
ACTGAACATG AACAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTACTAAAA 
TCTCGCCCGG CTCATTTGGC TGTTTTCTTA CACCATGTAG TTTCACAATT TGACCCTGCG 
ACTTTGCTCT GTTATCTCTA TTCAGACCTG TATAAACATA CCAATTCCAA AGAAACTCGT 
CGCATCTTCC TTGAGTTTCA TCAGTTCTTT CTAGATCGAT CAGCACACCT GAAAGTTTCT 
GTTCCTGATG AAATGTCTGC AGATCTAGAA AAGAGAAGAC CTGAGCTCAT TCCTGAGGAT 
CTGCATCGCC ACTATATCCA AACTATGCAA GAAAGAGTCC ATCCAGAAGT TCAAAGGCAC 
TTAGAAGATT TTCGGCAGAA ACGTAGTATG GGACTGACCT TGGCTGAAAG CGAGCTGACT 
AAACTTGATG CAGAGCGAGA CAAGGACCGA TTGACTTTGG AGAAGGAGCG GACATGTGCA 
GAACAGATTG TTGCCAAAAT TGAAGAAGTA TTGATGACTG CTCAGGCTGT AGAGGAAGAT 
AAGAGCTCCA CCATGCAGTA TGTTATTCTC ATGTATATGA AGCATTTGGG AGTAAAAGTG 
AAAGAGCCTC GAAATTTGGA GCACAAACGG GGTCGGATTG GATTTCTTCC CAAAATCAAG 
CAAAGTATGA AGAAAGATAA AGAAGGGGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC 
ATCCTGGGAC CCCCACGGAG ACCAAGCCGT CATGACAACA GTGCAATTGG CAGAGCCATG 
GAACTACAGA AGGCGCGCCA CCCTAAGCAC TTATCCACAC CCTCATCTGT GAGTCCTGAA 
CCTCAGGACT CTGCCAAGTT GCGCCAGAGT GGGTTAGCAA ATGAAGGAAC AGACGCTGGA 
TACCTGCCTG CCAATTCCAT GTCTTCTGTA GCTTCAGGGG CCTCTTTTTC CCAGGAAGGA 
GGGAAAGAGA ATGATACAGG ATCAAAGCAA GTTGGAGAAA CATCAGCACC TGGAGACACC 
TTAGATGGCA CACCTCGTAC TCTCAATACT GTCTTTGATT TCCCACCACC TCCATTAGAC 
CAAGTGCAGG AGGAGGAATG TGAAGTAGAA AGGGTGACAG AACATGGGAC ACCAAAGCCC 
TTTCGAAAGT TTGACAGTGT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAATTTGAA 
AATGACTTAG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGTCGAGA AGTGTTACTG 
GGACTAAAAC CTTGTGAAAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAA 
AGAGCTCATG TTCGAACACT GAAGGTTCTT GATCAAGTGT TCTATCAGCG AGTATCCAGA 
GAAGGAATTC TGTCACCCTC AGAGCTACGG AAAATTTTTT CAAACTTGGA AGATATTCTT 
CAACTTCATA TTGGATTGAA TGAACAAATG AAGGCTGTTC GAAAGAGAAA TGAGACCTCT 
GTTATCGATC AGATTGGGGA AGATTTGCTG ACATGGTTCA GCGGACCAGG AGAGGAGAAA 
TTGAAACATG CTGCTGCTAC CTTTTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAG 
TCTCGTCAGA AAAAGGATTC TCGATTTCAG ACTTTTGTGC AAGATGCTGA AAGTAATCCA 
CTGTGTCGTC GTCTTCAACT GAAGGATATT ATTCCCACTC AAATGCAAAG GCTTACTAAG 
TACCCACTTC TGTTGGATAA TATTGCCAAA TACACAGAAT GGCCAACAGA AAGGGAGAAG 
GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCCGTCAAG 
GAGGCAGAAA ACAAGCAGCG CCTAGAAGAT TATCAGCGTC GCCTTGATAC CTCCAGCCTG 
AAGTTGTCAG AGTACCCAAA TGTTGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG 
ATGATTCATG AAGGGCCATT GGTTTGGAAG GTGAATAGAG ATAAAACTAT TGATTTATAC 
ACATTGCTGC TGGAAGACAT TCTTGTATTG TTACAAAAGC AGGATGATAG ACTGGTTTTA 
AGGTGTCATA GTAAGATTCT GGCATCTACA GCTGATAGCA AACACACGTT TAGCCCTGTC 
ATTAAGTTGA GTACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTCGTC 
ATTTCCATGT CAGACAATGG CGCTCAGATT TATGAACTGG TGGCACAGAC AGTTTCTGAA 
AAGACTGTCT GGCAGGACCT AATCTGTCGG ATGGCTGCAT CAGTGAAGGA GCAATCCACA 
AAGCCAATTC CATTACCACA GTCAACACCT GGCGAAGGAG ATAATGATGA AGAAGATCCT 
TCAAAATTAA AAGAGGAGCA GCATGGCATT TCAGTCACTG GTTTGCAGAG TCCAGACAGA 
GATTTGGGAT TAGAATCTAC CTTAATATCG TCAAAACCTC AGTCTCATTC ACTGAGTACC 
TCTGGGAAAT CAGAGGTACG TGATCTGTTT GTGGCTGAGA GACAGTTTGC AAAGGAACAA 
CATACAGATG GGACACTAAA GGAAGTTGGA GAAGATTATC AAATCGCAAT CCCAGATTCA 
CACCTGCCTG TCTCAGAAGA ACGGTGGGCA TTGGATGCAC TAAGAAATTT GGGTTTGTTG 
AAGCAGTTGC TGGTGCAACA GCTAGGTTTG ACTGAGAAGA GCGTTCAGGA AGACTGGCAA 
CATTTCCCAA GATACAGAAC AGCCTCTCAG GGGCCGCAGA CAGACAGTGT CATCCAGAAC 
TCTGAAAATA TTAAGGCCTA TCATTCTGGT GAAGGACATA TGCCCTTTAG AACTGGAACT 
GGTGACATTG CAACGTGTTA CAGTCCACGG ACTTCAACTG AATCTTTTGC TCCACGGGAT 
TCAGTGGGAC TGGCACCCCA GGATAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG 
ATTATGACCC CAGAGATGCC TACCATGGAG CCAGAAGGGG GTCTTGATGA CAGTGGAGAG 
CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGAGAATC CATCAGAAGG TGATGGAGCA 
GTTAACAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTATTT GATCCTTGAT 
GGCTATGACC CAGTGCAGGA GAGTTCCACA GATGAGGAGG TTGCTTCCTC ACTTACCCTG 
CAGCCTATGA CAGGCATCCC TGCTGTGGAA TCCACCCACC AGCAGCAACA TTCTCCTCAG 
AATACTCACT CCGATGGGGC AATTTCACCA TTCACCCCCG AATTTCTGGT CCAGCAGCGC 
TGGGGAGCTA TGGAGTATTC CTGTTTTGAG ATCCAGAGTC CCTCCTCTTG TGCAGATTCA 
CAGAGCCAGA TCATGGAGTA CATTCATAAG ATAGAGGCTG ACCTTGAACA CTTAAAGAAG 
GTGGAGGAAA GTTACACCAT TCTTTGCCAA AGGCTGGCTG GATCAGCCCT CACAGACAAG 
CACTCAGATA AAAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016777646.1
CDS	339..4916
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERIASHDFDPTGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDVSRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_016922157.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016922157.1

ORF Insert Sequence:

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGGTCTTGTT CAGCGTTGCG TAATCATCCA GAAAGATGAC 
AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA 
GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA 
ACTCTGGTGA CTCACTCAAA TCATCTGGAG GTGGTGAAGC TAATCAAATC TGGTTCCTAT 
GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT TGCCGACTCT 
GAAGTAGAGC CGTCAGTCAT TGGACATATG TCTCCCATCA TGACATCTCC TCACTCACCT 
GGAGCATCTG GGAATATGGA GAGAATCACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT 
GTGGTTCATA ACCAGAAAGT AGAAATTCTC AGAAAAATGT TACAGAAAGA ACAGGAACGG 
CTACAGTTAT TGCAGGAAGA TTACAACCGA ACACCTGCCC AAAGATTGCT AAAAGAGATC 
CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT 
GCTCAGGATG GAGCTGTAGT TACACCCTCC AGACCTTTAG GGGACACCCT AACAGTCAGT 
GAGGCAGAAA CAGATCCTGG AGATGTACTG GGCAGGACTG ACTGTAGCAG TGGAGATGTT 
TCTCGGCCCA GTAGTGACAA TGCAGATAGT CCCAAGAGTG GCCCAAAAGA GAGAATTTAT 
CTAGAGGAAA ACCCAGAGAA AAGTGAAACA ATTCAGGACA CTGACACTCA ATCACTTGTC 
GGAAGTCCCT CAACCCGTAT AGCACCTCAT ATTATTGGAG CAGAAGATGA TGATTTTGGT 
ACTGAACATG AACAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTACTAAAA 
TCTCGCCCGG CTCATTTGGC TGTTTTCTTA CACCATGTAG TTTCACAATT TGACCCTGCG 
ACTTTGCTCT GTTATCTCTA TTCAGACCTG TATAAACATA CCAATTCCAA AGAAACTCGT 
CGCATCTTCC TTGAGTTTCA TCAGTTCTTT CTAGATCGAT CAGCACACCT GAAAGTTTCT 
GTTCCTGATG AAATGTCTGC AGATCTAGAA AAGAGAAGAC CTGAGCTCAT TCCTGAGGAT 
CTGCATCGCC ACTATATCCA AACTATGCAA GAAAGAGTCC ATCCAGAAGT TCAAAGGCAC 
TTAGAAGATT TTCGGCAGAA ACGTAGTATG GGACTGACCT TGGCTGAAAG CGAGCTGACT 
AAACTTGATG CAGAGCGAGA CAAGGACCGA TTGACTTTGG AGAAGGAGCG GACATGTGCA 
GAACAGATTG TTGCCAAAAT TGAAGAAGTA TTGATGACTG CTCAGGCTGT AGAGGAAGAT 
AAGAGCTCCA CCATGCAGTA TGTTATTCTC ATGTATATGA AGCATTTGGG AGTAAAAGTG 
AAAGAGCCTC GAAATTTGGA GCACAAACGG GGTCGGATTG GATTTCTTCC CAAAATCAAG 
CAAAGTATGA AGAAAGATAA AGAAGGGGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC 
ATCCTGGGAC CCCCACGGAG ACCAAGCCGT CATGACAACA GTGCAATTGG CAGAGCCATG 
GAACTACAGA AGGCGCGCCA CCCTAAGCAC TTATCCACAC CCTCATCTGT GAGTCCTGAA 
CCTCAGGACT CTGCCAAGTT GCGCCAGAGT GGGTTAGCAA ATGAAGGAAC AGACGCTGGA 
TACCTGCCTG CCAATTCCAT GTCTTCTGTA GCTTCAGGGG CCTCTTTTTC CCAGGAAGGA 
GGGAAAGAGA ATGATACAGG ATCAAAGCAA GTTGGAGAAA CATCAGCACC TGGAGACACC 
TTAGATGGCA CACCTCGTAC TCTCAATACT GTCTTTGATT TCCCACCACC TCCATTAGAC 
CAAGTGCAGG AGGAGGAATG TGAAGTAGAA AGGGTGACAG AACATGGGAC ACCAAAGCCC 
TTTCGAAAGT TTGACAGTGT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAATTTGAA 
AATGACTTAG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGTCGAGA AGTGTTACTG 
GGACTAAAAC CTTGTGAAAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAA 
AGAGCTCATG TTCGAACACT GAAGGTTCTT GATCAAGTGT TCTATCAGCG AGTATCCAGA 
GAAGGAATTC TGTCACCCTC AGAGCTACGG AAAATTTTTT CAAACTTGGA AGATATTCTT 
CAACTTCATA TTGGATTGAA TGAACAAATG AAGGCTGTTC GAAAGAGAAA TGAGACCTCT 
GTTATCGATC AGATTGGGGA AGATTTGCTG ACATGGTTCA GCGGACCAGG AGAGGAGAAA 
TTGAAACATG CTGCTGCTAC CTTTTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAG 
TCTCGTCAGA AAAAGGATTC TCGATTTCAG ACTTTTGTGC AAGATGCTGA AAGTAATCCA 
CTGTGTCGTC GTCTTCAACT GAAGGATATT ATTCCCACTC AAATGCAAAG GCTTACTAAG 
TACCCACTTC TGTTGGATAA TATTGCCAAA TACACAGAAT GGCCAACAGA AAGGGAGAAG 
GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCCGTCAAG 
GAGGCAGAAA ACAAGCAGCG CCTAGAAGAT TATCAGCGTC GCCTTGATAC CTCCAGCCTG 
AAGTTGTCAG AGTACCCAAA TGTTGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG 
ATGATTCATG AAGGGCCATT GGTTTGGAAG GTGAATAGAG ATAAAACTAT TGATTTATAC 
ACATTGCTGC TGGAAGACAT TCTTGTATTG TTACAAAAGC AGGATGATAG ACTGGTTTTA 
AGGTGTCATA GTAAGATTCT GGCATCTACA GCTGATAGCA AACACACGTT TAGCCCTGTC 
ATTAAGTTGA GTACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTCGTC 
ATTTCCATGT CAGACAATGG CGCTCAGATT TATGAACTGG TGGCACAGAC AGTTTCTGAA 
AAGACTGTCT GGCAGGACCT AATCTGTCGG ATGGCTGCAT CAGTGAAGGA GCAATCCACA 
AAGCCAATTC CATTACCACA GTCAACACCT GGCGAAGGAG ATAATGATGA AGAAGATCCT 
TCAAAATTAA AAGAGGAGCA GCATGGCATT TCAGTCACTG GTTTGCAGAG TCCAGACAGA 
GATTTGGGAT TAGAATCTAC CTTAATATCG TCAAAACCTC AGTCTCATTC ACTGAGTACC 
TCTGGGAAAT CAGAGGTACG TGATCTGTTT GTGGCTGAGA GACAGTTTGC AAAGGAACAA 
CATACAGATG GGACACTAAA GGAAGTTGGA GAAGATTATC AAATCGCAAT CCCAGATTCA 
CACCTGCCTG TCTCAGAAGA ACGGTGGGCA TTGGATGCAC TAAGAAATTT GGGTTTGTTG 
AAGCAGTTGC TGGTGCAACA GCTAGGTTTG ACTGAGAAGA GCGTTCAGGA AGACTGGCAA 
CATTTCCCAA GATACAGAAC AGCCTCTCAG GGGCCGCAGA CAGACAGTGT CATCCAGAAC 
TCTGAAAATA TTAAGGCCTA TCATTCTGGT GAAGGACATA TGCCCTTTAG AACTGGAACT 
GGTGACATTG CAACGTGTTA CAGTCCACGG ACTTCAACTG AATCTTTTGC TCCACGGGAT 
TCAGTGGGAC TGGCACCCCA GGATAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG 
ATTATGACCC CAGAGATGCC TACCATGGAG CCAGAAGGGG GTCTTGATGA CAGTGGAGAG 
CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGAGAATC CATCAGAAGG TGATGGAGCA 
GTTAACAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTATTT GATCCTTGAT 
GGCTATGACC CAGTGCAGGA GAGTTCCACA GATGAGGAGG TTGCTTCCTC ACTTACCCTG 
CAGCCTATGA CAGGCATCCC TGCTGTGGAA TCCACCCACC AGCAGCAACA TTCTCCTCAG 
AATACTCACT CCGATGGGGC AATTTCACCA TTCACCCCCG AATTTCTGGT CCAGCAGCGC 
TGGGGAGCTA TGGAGTATTC CTGTTTTGAG ATCCAGAGTC CCTCCTCTTG TGCAGATTCA 
CAGAGCCAGA TCATGGAGTA CATTCATAAG ATAGAGGCTG ACCTTGAACA CTTAAAGAAG 
GTGGAGGAAA GTTACACCAT TCTTTGCCAA AGGCTGGCTG GATCAGCCCT CACAGACAAG 
CACTCAGATA AAAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_016922157.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016777646.1 Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc64926
Clone ID Related Accession (Same CDS sequence)	XM_016922157.1 , XM_016922157.2
Accession Version	XM_016922157.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	rho guanine nucleotide exchange factor 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016922157.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGGTCTTGTT CAGCGTTGCG TAATCATCCA GAAAGATGAC 
AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA 
GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA 
ACTCTGGTGA CTCACTCAAA TCATCTGGAG GTGGTGAAGC TAATCAAATC TGGTTCCTAT 
GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT TGCCGACTCT 
GAAGTAGAGC CGTCAGTCAT TGGACATATG TCTCCCATCA TGACATCTCC TCACTCACCT 
GGAGCATCTG GGAATATGGA GAGAATCACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT 
GTGGTTCATA ACCAGAAAGT AGAAATTCTC AGAAAAATGT TACAGAAAGA ACAGGAACGG 
CTACAGTTAT TGCAGGAAGA TTACAACCGA ACACCTGCCC AAAGATTGCT AAAAGAGATC 
CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT 
GCTCAGGATG GAGCTGTAGT TACACCCTCC AGACCTTTAG GGGACACCCT AACAGTCAGT 
GAGGCAGAAA CAGATCCTGG AGATGTACTG GGCAGGACTG ACTGTAGCAG TGGAGATGTT 
TCTCGGCCCA GTAGTGACAA TGCAGATAGT CCCAAGAGTG GCCCAAAAGA GAGAATTTAT 
CTAGAGGAAA ACCCAGAGAA AAGTGAAACA ATTCAGGACA CTGACACTCA ATCACTTGTC 
GGAAGTCCCT CAACCCGTAT AGCACCTCAT ATTATTGGAG CAGAAGATGA TGATTTTGGT 
ACTGAACATG AACAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTACTAAAA 
TCTCGCCCGG CTCATTTGGC TGTTTTCTTA CACCATGTAG TTTCACAATT TGACCCTGCG 
ACTTTGCTCT GTTATCTCTA TTCAGACCTG TATAAACATA CCAATTCCAA AGAAACTCGT 
CGCATCTTCC TTGAGTTTCA TCAGTTCTTT CTAGATCGAT CAGCACACCT GAAAGTTTCT 
GTTCCTGATG AAATGTCTGC AGATCTAGAA AAGAGAAGAC CTGAGCTCAT TCCTGAGGAT 
CTGCATCGCC ACTATATCCA AACTATGCAA GAAAGAGTCC ATCCAGAAGT TCAAAGGCAC 
TTAGAAGATT TTCGGCAGAA ACGTAGTATG GGACTGACCT TGGCTGAAAG CGAGCTGACT 
AAACTTGATG CAGAGCGAGA CAAGGACCGA TTGACTTTGG AGAAGGAGCG GACATGTGCA 
GAACAGATTG TTGCCAAAAT TGAAGAAGTA TTGATGACTG CTCAGGCTGT AGAGGAAGAT 
AAGAGCTCCA CCATGCAGTA TGTTATTCTC ATGTATATGA AGCATTTGGG AGTAAAAGTG 
AAAGAGCCTC GAAATTTGGA GCACAAACGG GGTCGGATTG GATTTCTTCC CAAAATCAAG 
CAAAGTATGA AGAAAGATAA AGAAGGGGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC 
ATCCTGGGAC CCCCACGGAG ACCAAGCCGT CATGACAACA GTGCAATTGG CAGAGCCATG 
GAACTACAGA AGGCGCGCCA CCCTAAGCAC TTATCCACAC CCTCATCTGT GAGTCCTGAA 
CCTCAGGACT CTGCCAAGTT GCGCCAGAGT GGGTTAGCAA ATGAAGGAAC AGACGCTGGA 
TACCTGCCTG CCAATTCCAT GTCTTCTGTA GCTTCAGGGG CCTCTTTTTC CCAGGAAGGA 
GGGAAAGAGA ATGATACAGG ATCAAAGCAA GTTGGAGAAA CATCAGCACC TGGAGACACC 
TTAGATGGCA CACCTCGTAC TCTCAATACT GTCTTTGATT TCCCACCACC TCCATTAGAC 
CAAGTGCAGG AGGAGGAATG TGAAGTAGAA AGGGTGACAG AACATGGGAC ACCAAAGCCC 
TTTCGAAAGT TTGACAGTGT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAATTTGAA 
AATGACTTAG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGTCGAGA AGTGTTACTG 
GGACTAAAAC CTTGTGAAAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAA 
AGAGCTCATG TTCGAACACT GAAGGTTCTT GATCAAGTGT TCTATCAGCG AGTATCCAGA 
GAAGGAATTC TGTCACCCTC AGAGCTACGG AAAATTTTTT CAAACTTGGA AGATATTCTT 
CAACTTCATA TTGGATTGAA TGAACAAATG AAGGCTGTTC GAAAGAGAAA TGAGACCTCT 
GTTATCGATC AGATTGGGGA AGATTTGCTG ACATGGTTCA GCGGACCAGG AGAGGAGAAA 
TTGAAACATG CTGCTGCTAC CTTTTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAG 
TCTCGTCAGA AAAAGGATTC TCGATTTCAG ACTTTTGTGC AAGATGCTGA AAGTAATCCA 
CTGTGTCGTC GTCTTCAACT GAAGGATATT ATTCCCACTC AAATGCAAAG GCTTACTAAG 
TACCCACTTC TGTTGGATAA TATTGCCAAA TACACAGAAT GGCCAACAGA AAGGGAGAAG 
GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCCGTCAAG 
GAGGCAGAAA ACAAGCAGCG CCTAGAAGAT TATCAGCGTC GCCTTGATAC CTCCAGCCTG 
AAGTTGTCAG AGTACCCAAA TGTTGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG 
ATGATTCATG AAGGGCCATT GGTTTGGAAG GTGAATAGAG ATAAAACTAT TGATTTATAC 
ACATTGCTGC TGGAAGACAT TCTTGTATTG TTACAAAAGC AGGATGATAG ACTGGTTTTA 
AGGTGTCATA GTAAGATTCT GGCATCTACA GCTGATAGCA AACACACGTT TAGCCCTGTC 
ATTAAGTTGA GTACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTCGTC 
ATTTCCATGT CAGACAATGG CGCTCAGATT TATGAACTGG TGGCACAGAC AGTTTCTGAA 
AAGACTGTCT GGCAGGACCT AATCTGTCGG ATGGCTGCAT CAGTGAAGGA GCAATCCACA 
AAGCCAATTC CATTACCACA GTCAACACCT GGCGAAGGAG ATAATGATGA AGAAGATCCT 
TCAAAATTAA AAGAGGAGCA GCATGGCATT TCAGTCACTG GTTTGCAGAG TCCAGACAGA 
GATTTGGGAT TAGAATCTAC CTTAATATCG TCAAAACCTC AGTCTCATTC ACTGAGTACC 
TCTGGGAAAT CAGAGGTACG TGATCTGTTT GTGGCTGAGA GACAGTTTGC AAAGGAACAA 
CATACAGATG GGACACTAAA GGAAGTTGGA GAAGATTATC AAATCGCAAT CCCAGATTCA 
CACCTGCCTG TCTCAGAAGA ACGGTGGGCA TTGGATGCAC TAAGAAATTT GGGTTTGTTG 
AAGCAGTTGC TGGTGCAACA GCTAGGTTTG ACTGAGAAGA GCGTTCAGGA AGACTGGCAA 
CATTTCCCAA GATACAGAAC AGCCTCTCAG GGGCCGCAGA CAGACAGTGT CATCCAGAAC 
TCTGAAAATA TTAAGGCCTA TCATTCTGGT GAAGGACATA TGCCCTTTAG AACTGGAACT 
GGTGACATTG CAACGTGTTA CAGTCCACGG ACTTCAACTG AATCTTTTGC TCCACGGGAT 
TCAGTGGGAC TGGCACCCCA GGATAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG 
ATTATGACCC CAGAGATGCC TACCATGGAG CCAGAAGGGG GTCTTGATGA CAGTGGAGAG 
CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGAGAATC CATCAGAAGG TGATGGAGCA 
GTTAACAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTATTT GATCCTTGAT 
GGCTATGACC CAGTGCAGGA GAGTTCCACA GATGAGGAGG TTGCTTCCTC ACTTACCCTG 
CAGCCTATGA CAGGCATCCC TGCTGTGGAA TCCACCCACC AGCAGCAACA TTCTCCTCAG 
AATACTCACT CCGATGGGGC AATTTCACCA TTCACCCCCG AATTTCTGGT CCAGCAGCGC 
TGGGGAGCTA TGGAGTATTC CTGTTTTGAG ATCCAGAGTC CCTCCTCTTG TGCAGATTCA 
CAGAGCCAGA TCATGGAGTA CATTCATAAG ATAGAGGCTG ACCTTGAACA CTTAAAGAAG 
GTGGAGGAAA GTTACACCAT TCTTTGCCAA AGGCTGGCTG GATCAGCCCT CACAGACAAG 
CACTCAGATA AAAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016777646.1
CDS	339..4916
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERIASHDFDPTGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDVSRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_016922157.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016922157.2

ORF Insert Sequence:

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGGTCTTGTT CAGCGTTGCG TAATCATCCA GAAAGATGAC 
AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA 
GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA 
ACTCTGGTGA CTCACTCAAA TCATCTGGAG GTGGTGAAGC TAATCAAATC TGGTTCCTAT 
GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT TGCCGACTCT 
GAAGTAGAGC CGTCAGTCAT TGGACATATG TCTCCCATCA TGACATCTCC TCACTCACCT 
GGAGCATCTG GGAATATGGA GAGAATCACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT 
GTGGTTCATA ACCAGAAAGT AGAAATTCTC AGAAAAATGT TACAGAAAGA ACAGGAACGG 
CTACAGTTAT TGCAGGAAGA TTACAACCGA ACACCTGCCC AAAGATTGCT AAAAGAGATC 
CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT 
GCTCAGGATG GAGCTGTAGT TACACCCTCC AGACCTTTAG GGGACACCCT AACAGTCAGT 
GAGGCAGAAA CAGATCCTGG AGATGTACTG GGCAGGACTG ACTGTAGCAG TGGAGATGTT 
TCTCGGCCCA GTAGTGACAA TGCAGATAGT CCCAAGAGTG GCCCAAAAGA GAGAATTTAT 
CTAGAGGAAA ACCCAGAGAA AAGTGAAACA ATTCAGGACA CTGACACTCA ATCACTTGTC 
GGAAGTCCCT CAACCCGTAT AGCACCTCAT ATTATTGGAG CAGAAGATGA TGATTTTGGT 
ACTGAACATG AACAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTACTAAAA 
TCTCGCCCGG CTCATTTGGC TGTTTTCTTA CACCATGTAG TTTCACAATT TGACCCTGCG 
ACTTTGCTCT GTTATCTCTA TTCAGACCTG TATAAACATA CCAATTCCAA AGAAACTCGT 
CGCATCTTCC TTGAGTTTCA TCAGTTCTTT CTAGATCGAT CAGCACACCT GAAAGTTTCT 
GTTCCTGATG AAATGTCTGC AGATCTAGAA AAGAGAAGAC CTGAGCTCAT TCCTGAGGAT 
CTGCATCGCC ACTATATCCA AACTATGCAA GAAAGAGTCC ATCCAGAAGT TCAAAGGCAC 
TTAGAAGATT TTCGGCAGAA ACGTAGTATG GGACTGACCT TGGCTGAAAG CGAGCTGACT 
AAACTTGATG CAGAGCGAGA CAAGGACCGA TTGACTTTGG AGAAGGAGCG GACATGTGCA 
GAACAGATTG TTGCCAAAAT TGAAGAAGTA TTGATGACTG CTCAGGCTGT AGAGGAAGAT 
AAGAGCTCCA CCATGCAGTA TGTTATTCTC ATGTATATGA AGCATTTGGG AGTAAAAGTG 
AAAGAGCCTC GAAATTTGGA GCACAAACGG GGTCGGATTG GATTTCTTCC CAAAATCAAG 
CAAAGTATGA AGAAAGATAA AGAAGGGGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC 
ATCCTGGGAC CCCCACGGAG ACCAAGCCGT CATGACAACA GTGCAATTGG CAGAGCCATG 
GAACTACAGA AGGCGCGCCA CCCTAAGCAC TTATCCACAC CCTCATCTGT GAGTCCTGAA 
CCTCAGGACT CTGCCAAGTT GCGCCAGAGT GGGTTAGCAA ATGAAGGAAC AGACGCTGGA 
TACCTGCCTG CCAATTCCAT GTCTTCTGTA GCTTCAGGGG CCTCTTTTTC CCAGGAAGGA 
GGGAAAGAGA ATGATACAGG ATCAAAGCAA GTTGGAGAAA CATCAGCACC TGGAGACACC 
TTAGATGGCA CACCTCGTAC TCTCAATACT GTCTTTGATT TCCCACCACC TCCATTAGAC 
CAAGTGCAGG AGGAGGAATG TGAAGTAGAA AGGGTGACAG AACATGGGAC ACCAAAGCCC 
TTTCGAAAGT TTGACAGTGT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAATTTGAA 
AATGACTTAG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGTCGAGA AGTGTTACTG 
GGACTAAAAC CTTGTGAAAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAA 
AGAGCTCATG TTCGAACACT GAAGGTTCTT GATCAAGTGT TCTATCAGCG AGTATCCAGA 
GAAGGAATTC TGTCACCCTC AGAGCTACGG AAAATTTTTT CAAACTTGGA AGATATTCTT 
CAACTTCATA TTGGATTGAA TGAACAAATG AAGGCTGTTC GAAAGAGAAA TGAGACCTCT 
GTTATCGATC AGATTGGGGA AGATTTGCTG ACATGGTTCA GCGGACCAGG AGAGGAGAAA 
TTGAAACATG CTGCTGCTAC CTTTTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAG 
TCTCGTCAGA AAAAGGATTC TCGATTTCAG ACTTTTGTGC AAGATGCTGA AAGTAATCCA 
CTGTGTCGTC GTCTTCAACT GAAGGATATT ATTCCCACTC AAATGCAAAG GCTTACTAAG 
TACCCACTTC TGTTGGATAA TATTGCCAAA TACACAGAAT GGCCAACAGA AAGGGAGAAG 
GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCCGTCAAG 
GAGGCAGAAA ACAAGCAGCG CCTAGAAGAT TATCAGCGTC GCCTTGATAC CTCCAGCCTG 
AAGTTGTCAG AGTACCCAAA TGTTGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG 
ATGATTCATG AAGGGCCATT GGTTTGGAAG GTGAATAGAG ATAAAACTAT TGATTTATAC 
ACATTGCTGC TGGAAGACAT TCTTGTATTG TTACAAAAGC AGGATGATAG ACTGGTTTTA 
AGGTGTCATA GTAAGATTCT GGCATCTACA GCTGATAGCA AACACACGTT TAGCCCTGTC 
ATTAAGTTGA GTACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTCGTC 
ATTTCCATGT CAGACAATGG CGCTCAGATT TATGAACTGG TGGCACAGAC AGTTTCTGAA 
AAGACTGTCT GGCAGGACCT AATCTGTCGG ATGGCTGCAT CAGTGAAGGA GCAATCCACA 
AAGCCAATTC CATTACCACA GTCAACACCT GGCGAAGGAG ATAATGATGA AGAAGATCCT 
TCAAAATTAA AAGAGGAGCA GCATGGCATT TCAGTCACTG GTTTGCAGAG TCCAGACAGA 
GATTTGGGAT TAGAATCTAC CTTAATATCG TCAAAACCTC AGTCTCATTC ACTGAGTACC 
TCTGGGAAAT CAGAGGTACG TGATCTGTTT GTGGCTGAGA GACAGTTTGC AAAGGAACAA 
CATACAGATG GGACACTAAA GGAAGTTGGA GAAGATTATC AAATCGCAAT CCCAGATTCA 
CACCTGCCTG TCTCAGAAGA ACGGTGGGCA TTGGATGCAC TAAGAAATTT GGGTTTGTTG 
AAGCAGTTGC TGGTGCAACA GCTAGGTTTG ACTGAGAAGA GCGTTCAGGA AGACTGGCAA 
CATTTCCCAA GATACAGAAC AGCCTCTCAG GGGCCGCAGA CAGACAGTGT CATCCAGAAC 
TCTGAAAATA TTAAGGCCTA TCATTCTGGT GAAGGACATA TGCCCTTTAG AACTGGAACT 
GGTGACATTG CAACGTGTTA CAGTCCACGG ACTTCAACTG AATCTTTTGC TCCACGGGAT 
TCAGTGGGAC TGGCACCCCA GGATAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG 
ATTATGACCC CAGAGATGCC TACCATGGAG CCAGAAGGGG GTCTTGATGA CAGTGGAGAG 
CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGAGAATC CATCAGAAGG TGATGGAGCA 
GTTAACAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTATTT GATCCTTGAT 
GGCTATGACC CAGTGCAGGA GAGTTCCACA GATGAGGAGG TTGCTTCCTC ACTTACCCTG 
CAGCCTATGA CAGGCATCCC TGCTGTGGAA TCCACCCACC AGCAGCAACA TTCTCCTCAG 
AATACTCACT CCGATGGGGC AATTTCACCA TTCACCCCCG AATTTCTGGT CCAGCAGCGC 
TGGGGAGCTA TGGAGTATTC CTGTTTTGAG ATCCAGAGTC CCTCCTCTTG TGCAGATTCA 
CAGAGCCAGA TCATGGAGTA CATTCATAAG ATAGAGGCTG ACCTTGAACA CTTAAAGAAG 
GTGGAGGAAA GTTACACCAT TCTTTGCCAA AGGCTGGCTG GATCAGCCCT CACAGACAAG 
CACTCAGATA AAAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_016922158.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016777647.1 Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPc64927
Clone ID Related Accession (Same CDS sequence)	XM_016922158.2 , XM_016922158.1
Accession Version	XM_016922158.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	rho guanine nucleotide exchange factor 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036890.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016922158.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGCGTAA TCATCCAGAA AGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ACTCAAATCA TCTGGAGGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGACTCTGAA 
GTAGAGCCGT CAGTCATTGG ACATATGTCT CCCATCATGA CATCTCCTCA CTCACCTGGA 
GCATCTGGGA ATATGGAGAG AATCACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTCAGA AAAATGTTAC AGAAAGAACA GGAACGGCTA 
CAGGATGGAG CTGTAGTTAC ACCCTCCAGA CCTTTAGGGG ACACCCTAAC AGTCAGTGAG 
GCAGAAACAG ATCCTGGAGA TGTACTGGGC AGGACTGACT GTAGCAGTGG AGATGTTTCT 
CGGCCCAGTA GTGACAATGC AGATAGTCCC AAGAGTGGCC CAAAAGAGAG AATTTATCTA 
GAGGAAAACC CAGAGAAAAG TGAAACAATT CAGGACACTG ACACTCAATC ACTTGTCGGA 
AGTCCCTCAA CCCGTATAGC ACCTCATATT ATTGGAGCAG AAGATGATGA TTTTGGTACT 
GAACATGAAC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT ACTAAAATCT 
CGCCCGGCTC ATTTGGCTGT TTTCTTACAC CATGTAGTTT CACAATTTGA CCCTGCGACT 
TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAACATACCA ATTCCAAAGA AACTCGTCGC 
ATCTTCCTTG AGTTTCATCA GTTCTTTCTA GATCGATCAG CACACCTGAA AGTTTCTGTT 
CCTGATGAAA TGTCTGCAGA TCTAGAAAAG AGAAGACCTG AGCTCATTCC TGAGGATCTG 
CATCGCCACT ATATCCAAAC TATGCAAGAA AGAGTCCATC CAGAAGTTCA AAGGCACTTA 
GAAGATTTTC GGCAGAAACG TAGTATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA 
CTTGATGCAG AGCGAGACAA GGACCGATTG ACTTTGGAGA AGGAGCGGAC ATGTGCAGAA 
CAGATTGTTG CCAAAATTGA AGAAGTATTG ATGACTGCTC AGGCTGTAGA GGAAGATAAG 
AGCTCCACCA TGCAGTATGT TATTCTCATG TATATGAAGC ATTTGGGAGT AAAAGTGAAA 
GAGCCTCGAA ATTTGGAGCA CAAACGGGGT CGGATTGGAT TTCTTCCCAA AATCAAGCAA 
AGTATGAAGA AAGATAAAGA AGGGGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATC 
CTGGGACCCC CACGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCCATGGAA 
CTACAGAAGG CGCGCCACCC TAAGCACTTA TCCACACCCT CATCTGTGAG TCCTGAACCT 
CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAAATG AAGGAACAGA CGCTGGATAC 
CTGCCTGCCA ATTCCATGTC TTCTGTAGCT TCAGGGGCCT CTTTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAT CAGCACCTGG AGACACCTTA 
GATGGCACAC CTCGTACTCT CAATACTGTC TTTGATTTCC CACCACCTCC ATTAGACCAA 
GTGCAGGAGG AGGAATGTGA AGTAGAAAGG GTGACAGAAC ATGGGACACC AAAGCCCTTT 
CGAAAGTTTG ACAGTGTAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA ATTTGAAAAT 
GACTTAGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GTCGAGAAGT GTTACTGGGA 
CTAAAACCTT GTGAAATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ATCAGCGAGT ATCCAGAGAA 
GGAATTCTGT CACCCTCAGA GCTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA 
CTTCATATTG GATTGAATGA ACAAATGAAG GCTGTTCGAA AGAGAAATGA GACCTCTGTT 
ATCGATCAGA TTGGGGAAGA TTTGCTGACA TGGTTCAGCG GACCAGGAGA GGAGAAATTG 
AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAGTCT 
CGTCAGAAAA AGGATTCTCG ATTTCAGACT TTTGTGCAAG ATGCTGAAAG TAATCCACTG 
TGTCGTCGTC TTCAACTGAA GGATATTATT CCCACTCAAA TGCAAAGGCT TACTAAGTAC 
CCACTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAACAGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTATG TAAATCAGGC CGTCAAGGAG 
GCAGAAAACA AGCAGCGCCT AGAAGATTAT CAGCGTCGCC TTGATACCTC CAGCCTGAAG 
TTGTCAGAGT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCATGAAG GGCCATTGGT TTGGAAGGTG AATAGAGATA AAACTATTGA TTTATACACA 
TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTTTTAAGG 
TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT 
AAGTTGAGTA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATT 
TCCATGTCAG ACAATGGCGC TCAGATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAGCA ATCCACAAAG 
CCAATTCCAT TACCACAGTC AACACCTGGC GAAGGAGATA ATGATGAAGA AGATCCTTCA 
AAATTAAAAG AGGAGCAGCA TGGCATTTCA GTCACTGGTT TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AATCTACCTT AATATCGTCA AAACCTCAGT CTCATTCACT GAGTACCTCT 
GGGAAATCAG AGGTACGTGA TCTGTTTGTG GCTGAGAGAC AGTTTGCAAA GGAACAACAT 
ACAGATGGGA CACTAAAGGA AGTTGGAGAA GATTATCAAA TCGCAATCCC AGATTCACAC 
CTGCCTGTCT CAGAAGAACG GTGGGCATTG GATGCACTAA GAAATTTGGG TTTGTTGAAG 
CAGTTGCTGG TGCAACAGCT AGGTTTGACT GAGAAGAGCG TTCAGGAAGA CTGGCAACAT 
TTCCCAAGAT ACAGAACAGC CTCTCAGGGG CCGCAGACAG ACAGTGTCAT CCAGAACTCT 
GAAAATATTA AGGCCTATCA TTCTGGTGAA GGACATATGC CCTTTAGAAC TGGAACTGGT 
GACATTGCAA CGTGTTACAG TCCACGGACT TCAACTGAAT CTTTTGCTCC ACGGGATTCA 
GTGGGACTGG CACCCCAGGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTAC CATGGAGCCA GAAGGGGGTC TTGATGACAG TGGAGAGCAC 
TTTTTTGATG CCCGTGAAGC ACATAGTGAT GAGAATCCAT CAGAAGGTGA TGGAGCAGTT 
AACAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTATTTGAT CCTTGATGGC 
TATGACCCAG TGCAGGAGAG TTCCACAGAT GAGGAGGTTG CTTCCTCACT TACCCTGCAG 
CCTATGACAG GCATCCCTGC TGTGGAATCC ACCCACCAGC AGCAACATTC TCCTCAGAAT 
ACTCACTCCG ATGGGGCAAT TTCACCATTC ACCCCCGAAT TTCTGGTCCA GCAGCGCTGG 
GGAGCTATGG AGTATTCCTG TTTTGAGATC CAGAGTCCCT CCTCTTGTGC AGATTCACAG 
AGCCAGATCA TGGAGTACAT TCATAAGATA GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016777647.1
CDS	285..4799
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERIASHDFDPTDSSSKKTKSSSEESRSEIYGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDVSRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_016922158.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016922158.2

ORF Insert Sequence:

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGCGTAA TCATCCAGAA AGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ACTCAAATCA TCTGGAGGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGACTCTGAA 
GTAGAGCCGT CAGTCATTGG ACATATGTCT CCCATCATGA CATCTCCTCA CTCACCTGGA 
GCATCTGGGA ATATGGAGAG AATCACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTCAGA AAAATGTTAC AGAAAGAACA GGAACGGCTA 
CAGGATGGAG CTGTAGTTAC ACCCTCCAGA CCTTTAGGGG ACACCCTAAC AGTCAGTGAG 
GCAGAAACAG ATCCTGGAGA TGTACTGGGC AGGACTGACT GTAGCAGTGG AGATGTTTCT 
CGGCCCAGTA GTGACAATGC AGATAGTCCC AAGAGTGGCC CAAAAGAGAG AATTTATCTA 
GAGGAAAACC CAGAGAAAAG TGAAACAATT CAGGACACTG ACACTCAATC ACTTGTCGGA 
AGTCCCTCAA CCCGTATAGC ACCTCATATT ATTGGAGCAG AAGATGATGA TTTTGGTACT 
GAACATGAAC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT ACTAAAATCT 
CGCCCGGCTC ATTTGGCTGT TTTCTTACAC CATGTAGTTT CACAATTTGA CCCTGCGACT 
TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAACATACCA ATTCCAAAGA AACTCGTCGC 
ATCTTCCTTG AGTTTCATCA GTTCTTTCTA GATCGATCAG CACACCTGAA AGTTTCTGTT 
CCTGATGAAA TGTCTGCAGA TCTAGAAAAG AGAAGACCTG AGCTCATTCC TGAGGATCTG 
CATCGCCACT ATATCCAAAC TATGCAAGAA AGAGTCCATC CAGAAGTTCA AAGGCACTTA 
GAAGATTTTC GGCAGAAACG TAGTATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA 
CTTGATGCAG AGCGAGACAA GGACCGATTG ACTTTGGAGA AGGAGCGGAC ATGTGCAGAA 
CAGATTGTTG CCAAAATTGA AGAAGTATTG ATGACTGCTC AGGCTGTAGA GGAAGATAAG 
AGCTCCACCA TGCAGTATGT TATTCTCATG TATATGAAGC ATTTGGGAGT AAAAGTGAAA 
GAGCCTCGAA ATTTGGAGCA CAAACGGGGT CGGATTGGAT TTCTTCCCAA AATCAAGCAA 
AGTATGAAGA AAGATAAAGA AGGGGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATC 
CTGGGACCCC CACGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCCATGGAA 
CTACAGAAGG CGCGCCACCC TAAGCACTTA TCCACACCCT CATCTGTGAG TCCTGAACCT 
CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAAATG AAGGAACAGA CGCTGGATAC 
CTGCCTGCCA ATTCCATGTC TTCTGTAGCT TCAGGGGCCT CTTTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAT CAGCACCTGG AGACACCTTA 
GATGGCACAC CTCGTACTCT CAATACTGTC TTTGATTTCC CACCACCTCC ATTAGACCAA 
GTGCAGGAGG AGGAATGTGA AGTAGAAAGG GTGACAGAAC ATGGGACACC AAAGCCCTTT 
CGAAAGTTTG ACAGTGTAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA ATTTGAAAAT 
GACTTAGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GTCGAGAAGT GTTACTGGGA 
CTAAAACCTT GTGAAATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ATCAGCGAGT ATCCAGAGAA 
GGAATTCTGT CACCCTCAGA GCTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA 
CTTCATATTG GATTGAATGA ACAAATGAAG GCTGTTCGAA AGAGAAATGA GACCTCTGTT 
ATCGATCAGA TTGGGGAAGA TTTGCTGACA TGGTTCAGCG GACCAGGAGA GGAGAAATTG 
AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAGTCT 
CGTCAGAAAA AGGATTCTCG ATTTCAGACT TTTGTGCAAG ATGCTGAAAG TAATCCACTG 
TGTCGTCGTC TTCAACTGAA GGATATTATT CCCACTCAAA TGCAAAGGCT TACTAAGTAC 
CCACTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAACAGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTATG TAAATCAGGC CGTCAAGGAG 
GCAGAAAACA AGCAGCGCCT AGAAGATTAT CAGCGTCGCC TTGATACCTC CAGCCTGAAG 
TTGTCAGAGT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCATGAAG GGCCATTGGT TTGGAAGGTG AATAGAGATA AAACTATTGA TTTATACACA 
TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTTTTAAGG 
TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT 
AAGTTGAGTA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATT 
TCCATGTCAG ACAATGGCGC TCAGATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAGCA ATCCACAAAG 
CCAATTCCAT TACCACAGTC AACACCTGGC GAAGGAGATA ATGATGAAGA AGATCCTTCA 
AAATTAAAAG AGGAGCAGCA TGGCATTTCA GTCACTGGTT TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AATCTACCTT AATATCGTCA AAACCTCAGT CTCATTCACT GAGTACCTCT 
GGGAAATCAG AGGTACGTGA TCTGTTTGTG GCTGAGAGAC AGTTTGCAAA GGAACAACAT 
ACAGATGGGA CACTAAAGGA AGTTGGAGAA GATTATCAAA TCGCAATCCC AGATTCACAC 
CTGCCTGTCT CAGAAGAACG GTGGGCATTG GATGCACTAA GAAATTTGGG TTTGTTGAAG 
CAGTTGCTGG TGCAACAGCT AGGTTTGACT GAGAAGAGCG TTCAGGAAGA CTGGCAACAT 
TTCCCAAGAT ACAGAACAGC CTCTCAGGGG CCGCAGACAG ACAGTGTCAT CCAGAACTCT 
GAAAATATTA AGGCCTATCA TTCTGGTGAA GGACATATGC CCTTTAGAAC TGGAACTGGT 
GACATTGCAA CGTGTTACAG TCCACGGACT TCAACTGAAT CTTTTGCTCC ACGGGATTCA 
GTGGGACTGG CACCCCAGGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTAC CATGGAGCCA GAAGGGGGTC TTGATGACAG TGGAGAGCAC 
TTTTTTGATG CCCGTGAAGC ACATAGTGAT GAGAATCCAT CAGAAGGTGA TGGAGCAGTT 
AACAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTATTTGAT CCTTGATGGC 
TATGACCCAG TGCAGGAGAG TTCCACAGAT GAGGAGGTTG CTTCCTCACT TACCCTGCAG 
CCTATGACAG GCATCCCTGC TGTGGAATCC ACCCACCAGC AGCAACATTC TCCTCAGAAT 
ACTCACTCCG ATGGGGCAAT TTCACCATTC ACCCCCGAAT TTCTGGTCCA GCAGCGCTGG 
GGAGCTATGG AGTATTCCTG TTTTGAGATC CAGAGTCCCT CCTCTTGTGC AGATTCACAG 
AGCCAGATCA TGGAGTACAT TCATAAGATA GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_016922158.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016777647.1 Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA.

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CloneID	OPc64927
Clone ID Related Accession (Same CDS sequence)	XM_016922158.2 , XM_016922158.1
Accession Version	XM_016922158.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	rho guanine nucleotide exchange factor 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006478.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGCGTAA TCATCCAGAA AGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ACTCAAATCA TCTGGAGGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGACTCTGAA 
GTAGAGCCGT CAGTCATTGG ACATATGTCT CCCATCATGA CATCTCCTCA CTCACCTGGA 
GCATCTGGGA ATATGGAGAG AATCACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTCAGA AAAATGTTAC AGAAAGAACA GGAACGGCTA 
CAGGATGGAG CTGTAGTTAC ACCCTCCAGA CCTTTAGGGG ACACCCTAAC AGTCAGTGAG 
GCAGAAACAG ATCCTGGAGA TGTACTGGGC AGGACTGACT GTAGCAGTGG AGATGTTTCT 
CGGCCCAGTA GTGACAATGC AGATAGTCCC AAGAGTGGCC CAAAAGAGAG AATTTATCTA 
GAGGAAAACC CAGAGAAAAG TGAAACAATT CAGGACACTG ACACTCAATC ACTTGTCGGA 
AGTCCCTCAA CCCGTATAGC ACCTCATATT ATTGGAGCAG AAGATGATGA TTTTGGTACT 
GAACATGAAC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT ACTAAAATCT 
CGCCCGGCTC ATTTGGCTGT TTTCTTACAC CATGTAGTTT CACAATTTGA CCCTGCGACT 
TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAACATACCA ATTCCAAAGA AACTCGTCGC 
ATCTTCCTTG AGTTTCATCA GTTCTTTCTA GATCGATCAG CACACCTGAA AGTTTCTGTT 
CCTGATGAAA TGTCTGCAGA TCTAGAAAAG AGAAGACCTG AGCTCATTCC TGAGGATCTG 
CATCGCCACT ATATCCAAAC TATGCAAGAA AGAGTCCATC CAGAAGTTCA AAGGCACTTA 
GAAGATTTTC GGCAGAAACG TAGTATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA 
CTTGATGCAG AGCGAGACAA GGACCGATTG ACTTTGGAGA AGGAGCGGAC ATGTGCAGAA 
CAGATTGTTG CCAAAATTGA AGAAGTATTG ATGACTGCTC AGGCTGTAGA GGAAGATAAG 
AGCTCCACCA TGCAGTATGT TATTCTCATG TATATGAAGC ATTTGGGAGT AAAAGTGAAA 
GAGCCTCGAA ATTTGGAGCA CAAACGGGGT CGGATTGGAT TTCTTCCCAA AATCAAGCAA 
AGTATGAAGA AAGATAAAGA AGGGGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATC 
CTGGGACCCC CACGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCCATGGAA 
CTACAGAAGG CGCGCCACCC TAAGCACTTA TCCACACCCT CATCTGTGAG TCCTGAACCT 
CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAAATG AAGGAACAGA CGCTGGATAC 
CTGCCTGCCA ATTCCATGTC TTCTGTAGCT TCAGGGGCCT CTTTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAT CAGCACCTGG AGACACCTTA 
GATGGCACAC CTCGTACTCT CAATACTGTC TTTGATTTCC CACCACCTCC ATTAGACCAA 
GTGCAGGAGG AGGAATGTGA AGTAGAAAGG GTGACAGAAC ATGGGACACC AAAGCCCTTT 
CGAAAGTTTG ACAGTGTAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA ATTTGAAAAT 
GACTTAGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GTCGAGAAGT GTTACTGGGA 
CTAAAACCTT GTGAAATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ATCAGCGAGT ATCCAGAGAA 
GGAATTCTGT CACCCTCAGA GCTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA 
CTTCATATTG GATTGAATGA ACAAATGAAG GCTGTTCGAA AGAGAAATGA GACCTCTGTT 
ATCGATCAGA TTGGGGAAGA TTTGCTGACA TGGTTCAGCG GACCAGGAGA GGAGAAATTG 
AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAGTCT 
CGTCAGAAAA AGGATTCTCG ATTTCAGACT TTTGTGCAAG ATGCTGAAAG TAATCCACTG 
TGTCGTCGTC TTCAACTGAA GGATATTATT CCCACTCAAA TGCAAAGGCT TACTAAGTAC 
CCACTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAACAGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTATG TAAATCAGGC CGTCAAGGAG 
GCAGAAAACA AGCAGCGCCT AGAAGATTAT CAGCGTCGCC TTGATACCTC CAGCCTGAAG 
TTGTCAGAGT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCATGAAG GGCCATTGGT TTGGAAGGTG AATAGAGATA AAACTATTGA TTTATACACA 
TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTTTTAAGG 
TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT 
AAGTTGAGTA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATT 
TCCATGTCAG ACAATGGCGC TCAGATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAGCA ATCCACAAAG 
CCAATTCCAT TACCACAGTC AACACCTGGC GAAGGAGATA ATGATGAAGA AGATCCTTCA 
AAATTAAAAG AGGAGCAGCA TGGCATTTCA GTCACTGGTT TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AATCTACCTT AATATCGTCA AAACCTCAGT CTCATTCACT GAGTACCTCT 
GGGAAATCAG AGGTACGTGA TCTGTTTGTG GCTGAGAGAC AGTTTGCAAA GGAACAACAT 
ACAGATGGGA CACTAAAGGA AGTTGGAGAA GATTATCAAA TCGCAATCCC AGATTCACAC 
CTGCCTGTCT CAGAAGAACG GTGGGCATTG GATGCACTAA GAAATTTGGG TTTGTTGAAG 
CAGTTGCTGG TGCAACAGCT AGGTTTGACT GAGAAGAGCG TTCAGGAAGA CTGGCAACAT 
TTCCCAAGAT ACAGAACAGC CTCTCAGGGG CCGCAGACAG ACAGTGTCAT CCAGAACTCT 
GAAAATATTA AGGCCTATCA TTCTGGTGAA GGACATATGC CCTTTAGAAC TGGAACTGGT 
GACATTGCAA CGTGTTACAG TCCACGGACT TCAACTGAAT CTTTTGCTCC ACGGGATTCA 
GTGGGACTGG CACCCCAGGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTAC CATGGAGCCA GAAGGGGGTC TTGATGACAG TGGAGAGCAC 
TTTTTTGATG CCCGTGAAGC ACATAGTGAT GAGAATCCAT CAGAAGGTGA TGGAGCAGTT 
AACAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTATTTGAT CCTTGATGGC 
TATGACCCAG TGCAGGAGAG TTCCACAGAT GAGGAGGTTG CTTCCTCACT TACCCTGCAG 
CCTATGACAG GCATCCCTGC TGTGGAATCC ACCCACCAGC AGCAACATTC TCCTCAGAAT 
ACTCACTCCG ATGGGGCAAT TTCACCATTC ACCCCCGAAT TTCTGGTCCA GCAGCGCTGG 
GGAGCTATGG AGTATTCCTG TTTTGAGATC CAGAGTCCCT CCTCTTGTGC AGATTCACAG 
AGCCAGATCA TGGAGTACAT TCATAAGATA GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016777647.1
CDS	285..4799
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERIASHDFDPTDSSSKKTKSSSEESRSEIYGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDVSRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_016922158.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016922158.1

ORF Insert Sequence:

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGCGTAA TCATCCAGAA AGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ACTCAAATCA TCTGGAGGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGACTCTGAA 
GTAGAGCCGT CAGTCATTGG ACATATGTCT CCCATCATGA CATCTCCTCA CTCACCTGGA 
GCATCTGGGA ATATGGAGAG AATCACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTCAGA AAAATGTTAC AGAAAGAACA GGAACGGCTA 
CAGGATGGAG CTGTAGTTAC ACCCTCCAGA CCTTTAGGGG ACACCCTAAC AGTCAGTGAG 
GCAGAAACAG ATCCTGGAGA TGTACTGGGC AGGACTGACT GTAGCAGTGG AGATGTTTCT 
CGGCCCAGTA GTGACAATGC AGATAGTCCC AAGAGTGGCC CAAAAGAGAG AATTTATCTA 
GAGGAAAACC CAGAGAAAAG TGAAACAATT CAGGACACTG ACACTCAATC ACTTGTCGGA 
AGTCCCTCAA CCCGTATAGC ACCTCATATT ATTGGAGCAG AAGATGATGA TTTTGGTACT 
GAACATGAAC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT ACTAAAATCT 
CGCCCGGCTC ATTTGGCTGT TTTCTTACAC CATGTAGTTT CACAATTTGA CCCTGCGACT 
TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAACATACCA ATTCCAAAGA AACTCGTCGC 
ATCTTCCTTG AGTTTCATCA GTTCTTTCTA GATCGATCAG CACACCTGAA AGTTTCTGTT 
CCTGATGAAA TGTCTGCAGA TCTAGAAAAG AGAAGACCTG AGCTCATTCC TGAGGATCTG 
CATCGCCACT ATATCCAAAC TATGCAAGAA AGAGTCCATC CAGAAGTTCA AAGGCACTTA 
GAAGATTTTC GGCAGAAACG TAGTATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA 
CTTGATGCAG AGCGAGACAA GGACCGATTG ACTTTGGAGA AGGAGCGGAC ATGTGCAGAA 
CAGATTGTTG CCAAAATTGA AGAAGTATTG ATGACTGCTC AGGCTGTAGA GGAAGATAAG 
AGCTCCACCA TGCAGTATGT TATTCTCATG TATATGAAGC ATTTGGGAGT AAAAGTGAAA 
GAGCCTCGAA ATTTGGAGCA CAAACGGGGT CGGATTGGAT TTCTTCCCAA AATCAAGCAA 
AGTATGAAGA AAGATAAAGA AGGGGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATC 
CTGGGACCCC CACGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCCATGGAA 
CTACAGAAGG CGCGCCACCC TAAGCACTTA TCCACACCCT CATCTGTGAG TCCTGAACCT 
CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAAATG AAGGAACAGA CGCTGGATAC 
CTGCCTGCCA ATTCCATGTC TTCTGTAGCT TCAGGGGCCT CTTTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAT CAGCACCTGG AGACACCTTA 
GATGGCACAC CTCGTACTCT CAATACTGTC TTTGATTTCC CACCACCTCC ATTAGACCAA 
GTGCAGGAGG AGGAATGTGA AGTAGAAAGG GTGACAGAAC ATGGGACACC AAAGCCCTTT 
CGAAAGTTTG ACAGTGTAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA ATTTGAAAAT 
GACTTAGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GTCGAGAAGT GTTACTGGGA 
CTAAAACCTT GTGAAATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ATCAGCGAGT ATCCAGAGAA 
GGAATTCTGT CACCCTCAGA GCTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA 
CTTCATATTG GATTGAATGA ACAAATGAAG GCTGTTCGAA AGAGAAATGA GACCTCTGTT 
ATCGATCAGA TTGGGGAAGA TTTGCTGACA TGGTTCAGCG GACCAGGAGA GGAGAAATTG 
AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAGTCT 
CGTCAGAAAA AGGATTCTCG ATTTCAGACT TTTGTGCAAG ATGCTGAAAG TAATCCACTG 
TGTCGTCGTC TTCAACTGAA GGATATTATT CCCACTCAAA TGCAAAGGCT TACTAAGTAC 
CCACTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAACAGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTATG TAAATCAGGC CGTCAAGGAG 
GCAGAAAACA AGCAGCGCCT AGAAGATTAT CAGCGTCGCC TTGATACCTC CAGCCTGAAG 
TTGTCAGAGT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCATGAAG GGCCATTGGT TTGGAAGGTG AATAGAGATA AAACTATTGA TTTATACACA 
TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTTTTAAGG 
TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT 
AAGTTGAGTA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATT 
TCCATGTCAG ACAATGGCGC TCAGATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAGCA ATCCACAAAG 
CCAATTCCAT TACCACAGTC AACACCTGGC GAAGGAGATA ATGATGAAGA AGATCCTTCA 
AAATTAAAAG AGGAGCAGCA TGGCATTTCA GTCACTGGTT TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AATCTACCTT AATATCGTCA AAACCTCAGT CTCATTCACT GAGTACCTCT 
GGGAAATCAG AGGTACGTGA TCTGTTTGTG GCTGAGAGAC AGTTTGCAAA GGAACAACAT 
ACAGATGGGA CACTAAAGGA AGTTGGAGAA GATTATCAAA TCGCAATCCC AGATTCACAC 
CTGCCTGTCT CAGAAGAACG GTGGGCATTG GATGCACTAA GAAATTTGGG TTTGTTGAAG 
CAGTTGCTGG TGCAACAGCT AGGTTTGACT GAGAAGAGCG TTCAGGAAGA CTGGCAACAT 
TTCCCAAGAT ACAGAACAGC CTCTCAGGGG CCGCAGACAG ACAGTGTCAT CCAGAACTCT 
GAAAATATTA AGGCCTATCA TTCTGGTGAA GGACATATGC CCTTTAGAAC TGGAACTGGT 
GACATTGCAA CGTGTTACAG TCCACGGACT TCAACTGAAT CTTTTGCTCC ACGGGATTCA 
GTGGGACTGG CACCCCAGGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTAC CATGGAGCCA GAAGGGGGTC TTGATGACAG TGGAGAGCAC 
TTTTTTGATG CCCGTGAAGC ACATAGTGAT GAGAATCCAT CAGAAGGTGA TGGAGCAGTT 
AACAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTATTTGAT CCTTGATGGC 
TATGACCCAG TGCAGGAGAG TTCCACAGAT GAGGAGGTTG CTTCCTCACT TACCCTGCAG 
CCTATGACAG GCATCCCTGC TGTGGAATCC ACCCACCAGC AGCAACATTC TCCTCAGAAT 
ACTCACTCCG ATGGGGCAAT TTCACCATTC ACCCCCGAAT TTCTGGTCCA GCAGCGCTGG 
GGAGCTATGG AGTATTCCTG TTTTGAGATC CAGAGTCCCT CCTCTTGTGC AGATTCACAG 
AGCCAGATCA TGGAGTACAT TCATAAGATA GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ARHGEF12 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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