LOC112286231 cDNA ORF clone,

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gRNAs

The following LOC112286231 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC112286231 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPn51100	XM_024527930.1 Latest version!	Physcomitrella patens probable clathrin assembly protein At4g32285 (LOC112286231), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC112286231 ( XM_024527930.1 ) cDNA ORF clone, -> XP_024383698.1 Physcomitrella patens probable clathrin assembly protein At4g32285 (LOC112286231), mRNA.

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CloneID	OPn51100
Clone ID Related Accession (Same CDS sequence)	XM_024527930.1
Accession Version	XM_024527930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-03
Organism	Physcomitrella patens
Product	probable clathrin assembly protein At4g32285
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037261.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Physcomitrella patens Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATTG CACCCAAAAA GATTCGTAAA GCAATAGGCG GTCTGAAGGA TCAGACCAGC 
ATTGGCTTTG CGAAGGTAGG TGGCGCGAGA GCTGCAGACC TCGACGTCGC ACTTGTGAAG 
GCAACCAGCC ACGATGATTA CTTCGACGAG AAATATGTTC AGGAGATTTT GCACCTCACC 
TCTCACTCCC GCGGCTACGT GAGTGCATGC GTCACCAGCG TGGGCAGGAG ATTGACTAAG 
ACACACGACT GGAATGTGGC TTTGAAGGGT CTCATGCTCT GTCACCGTCT CCTCCGTGAT 
GGTGATCCCA GCTTTGAAAA CGAACTGATG CATGCCACCC GACGTGGTCG CCGCATTCTA 
AACCTCTCAA ACTTTAAGGA TGAAACGCAT TCAAACGCTT GGGATTATAG CTCTTTTGTT 
CGGACATATG GATTGTTTCT CGATGAGCGT TTGGATTGCT CATTGAAAGT TTCCGGCAAG 
AACAAGAACC GTAGAGGTAG AGGCGAGAGA GGAAGCAGGG GCAGGTCTTC ACATAGCAAA 
TCTCCTGTGG AATCGAGCTA TCGAAACTCT CCAGATCGGT ATGCTCGATC TCGGTGTGGT 
GGCTCTCCTG ATTCTCGTGC ATATAGCATA GCAACCACGA ACGATAACCG GCGGCACAGC 
AATTACGACG GGGAACTTTC GCCACGGTAT AGCAATAGGT CAGGGCGCAA AGATTCGTGG 
AAAGATGACA GATCCGATGA CAAGGAAGAA GATTCTGACA ATGTGCCAAT CAAGGAGATG 
AACGTCAAGC AGTTGCTAGT CAATCTTCCT GCCATGCAGA GATTGATGGA CCGGGTGCTT 
GGATGCCGGC CCGCAGGTGC TGCAAAGACA AACCGCTTGG TCCAGCATGC TCTGTACCTT 
ATTATTAAGG AGAGCTTCCA GCTGCATAGG GATATTTGTG ACGGCAGTGC AGTTCTTCTC 
GAGGCTTTTT TCGACATGGA CCAGAAGGAC AGGGTTAAAG CCTACGAAAG CTTCTACACC 
TCTGCCAAAC AAGCGGACGA GCTACATGAG TTTCTCAATT TGTGCAAGCA TCATGGTATT 
GGTCGGTCTT CGGAGTACAT CGAAGTCGCA CCTGTTCCGA AGGAGCAACT GGATAATCTT 
GAGGAATATT TGCGATCAAA CGCACCAACC CGAACCAGGA GCAAGAGCCC CGAGGCTCCG 
ACTTTGCAAC TCGAGTACAA GCCTCGAACT CCTGAGCATT CACCAGAATC TGAACCTGTT 
CCGAAAGAAG ACGCTCCCGA GGTTGTAGTA GAACCAGAGC CAGCGCCAGC CCCTGCACCA 
GCCCCTGTCC CAGCTCCAAT AGCCGCTGTC GGAGATTTGT TAGACATGGA CAATGCAACA 
ATATCTACCG AAGATCACAG CGAGAAACTC GCTTTGGCCC TTTTCTCTAC TTCAACCACC 
ACTAGTACCT GGGAAACCTT CAATTCGGAC GATAAACAAA GTTCCCTTCA AGCATTTAAT 
GCCTCAGAAT CTGGAAAAGC TGGTTGGGAG CTGGCCTTGG TAGAATCTGC AAGCAATCTG 
TCGAAGCCCG CCCCTGACCG ACCCTTGGCT GGTGGTTTTG ACAACCTACT TTTGGACAGC 
ATGTACAACC AGGGTGAAGT TCTGCAGAAA CAAGCTGTCG CTGCAGCTCC AACGGGTAGC 
GCAAGCAGTG TGGCTATTCC TAACCGCCCT TCTTCTGCCT TCCTCGCTTT GCCTGCTCCT 
CCCGGAGCAA TGTCATTGCC TCTGAACGGG GATGACCCCT TCGCAGCATC TGCTGTTGTT 
CCTCCTCCTG CGTATGTGCA GATGGCAGAT CTGGACACGA AGCAACAACT CTTATCTCAA 
GAACAAATCA TGTGGCAACG ATACCAGATG GAGGGTATGC GGGGCGAGGC GACTTTCCAG 
AAAGTGTTGA ACAATCCCTA CGTTTGCATG CCAGGTCCTG GCCCAATGAT GCCACCCTAC 
CAAAACCCTT ACACTGTTGG CATGCCCGTG GGATACCAAA CACCGTATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024383698.1
CDS	514..2544
Translation	MAIAPKKIRKAIGGLKDQTSIGFAKVGGARAADLDVALVKATSHDDYFDEKYVQEILHLTSHSRGYVSACVTSVGRRLTKTHDWNVALKGLMLCHRLLRDGDPSFENELMHATRRGRRILNLSNFKDETHSNAWDYSSFVRTYGLFLDERLDCSLKVSGKNKNRRGRGERGSRGRSSHSKSPVESSYRNSPDRYARSRCGGSPDSRAYSIATTNDNRRHSNYDGELSPRYSNRSGRKDSWKDDRSDDKEEDSDNVPIKEMNVKQLLVNLPAMQRLMDRVLGCRPAGAAKTNRLVQHALYLIIKESFQLHRDICDGSAVLLEAFFDMDQKDRVKAYESFYTSAKQADELHEFLNLCKHHGIGRSSEYIEVAPVPKEQLDNLEEYLRSNAPTRTRSKSPEAPTLQLEYKPRTPEHSPESEPVPKEDAPEVVVEPEPAPAPAPAPVPAPIAAVGDLLDMDNATISTEDHSEKLALALFSTSTTTSTWETFNSDDKQSSLQAFNASESGKAGWELALVESASNLSKPAPDRPLAGGFDNLLLDSMYNQGEVLQKQAVAAAPTGSASSVAIPNRPSSAFLALPAPPGAMSLPLNGDDPFAASAVVPPPAYVQMADLDTKQQLLSQEQIMWQRYQMEGMRGEATFQKVLNNPYVCMPGPGPMMPPYQNPYTVGMPVGYQTPY

Target ORF information:

RefSeq Version	XM_024527930.1
Organism	Physcomitrella patens
Definition	Physcomitrella patens probable clathrin assembly protein At4g32285 (LOC112286231), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024527930.1

ORF Insert Sequence:

ATGGCGATTG CACCCAAAAA GATTCGTAAA GCAATAGGCG GTCTGAAGGA TCAGACCAGC 
ATTGGCTTTG CGAAGGTAGG TGGCGCGAGA GCTGCAGACC TCGACGTCGC ACTTGTGAAG 
GCAACCAGCC ACGATGATTA CTTCGACGAG AAATATGTTC AGGAGATTTT GCACCTCACC 
TCTCACTCCC GCGGCTACGT GAGTGCATGC GTCACCAGCG TGGGCAGGAG ATTGACTAAG 
ACACACGACT GGAATGTGGC TTTGAAGGGT CTCATGCTCT GTCACCGTCT CCTCCGTGAT 
GGTGATCCCA GCTTTGAAAA CGAACTGATG CATGCCACCC GACGTGGTCG CCGCATTCTA 
AACCTCTCAA ACTTTAAGGA TGAAACGCAT TCAAACGCTT GGGATTATAG CTCTTTTGTT 
CGGACATATG GATTGTTTCT CGATGAGCGT TTGGATTGCT CATTGAAAGT TTCCGGCAAG 
AACAAGAACC GTAGAGGTAG AGGCGAGAGA GGAAGCAGGG GCAGGTCTTC ACATAGCAAA 
TCTCCTGTGG AATCGAGCTA TCGAAACTCT CCAGATCGGT ATGCTCGATC TCGGTGTGGT 
GGCTCTCCTG ATTCTCGTGC ATATAGCATA GCAACCACGA ACGATAACCG GCGGCACAGC 
AATTACGACG GGGAACTTTC GCCACGGTAT AGCAATAGGT CAGGGCGCAA AGATTCGTGG 
AAAGATGACA GATCCGATGA CAAGGAAGAA GATTCTGACA ATGTGCCAAT CAAGGAGATG 
AACGTCAAGC AGTTGCTAGT CAATCTTCCT GCCATGCAGA GATTGATGGA CCGGGTGCTT 
GGATGCCGGC CCGCAGGTGC TGCAAAGACA AACCGCTTGG TCCAGCATGC TCTGTACCTT 
ATTATTAAGG AGAGCTTCCA GCTGCATAGG GATATTTGTG ACGGCAGTGC AGTTCTTCTC 
GAGGCTTTTT TCGACATGGA CCAGAAGGAC AGGGTTAAAG CCTACGAAAG CTTCTACACC 
TCTGCCAAAC AAGCGGACGA GCTACATGAG TTTCTCAATT TGTGCAAGCA TCATGGTATT 
GGTCGGTCTT CGGAGTACAT CGAAGTCGCA CCTGTTCCGA AGGAGCAACT GGATAATCTT 
GAGGAATATT TGCGATCAAA CGCACCAACC CGAACCAGGA GCAAGAGCCC CGAGGCTCCG 
ACTTTGCAAC TCGAGTACAA GCCTCGAACT CCTGAGCATT CACCAGAATC TGAACCTGTT 
CCGAAAGAAG ACGCTCCCGA GGTTGTAGTA GAACCAGAGC CAGCGCCAGC CCCTGCACCA 
GCCCCTGTCC CAGCTCCAAT AGCCGCTGTC GGAGATTTGT TAGACATGGA CAATGCAACA 
ATATCTACCG AAGATCACAG CGAGAAACTC GCTTTGGCCC TTTTCTCTAC TTCAACCACC 
ACTAGTACCT GGGAAACCTT CAATTCGGAC GATAAACAAA GTTCCCTTCA AGCATTTAAT 
GCCTCAGAAT CTGGAAAAGC TGGTTGGGAG CTGGCCTTGG TAGAATCTGC AAGCAATCTG 
TCGAAGCCCG CCCCTGACCG ACCCTTGGCT GGTGGTTTTG ACAACCTACT TTTGGACAGC 
ATGTACAACC AGGGTGAAGT TCTGCAGAAA CAAGCTGTCG CTGCAGCTCC AACGGGTAGC 
GCAAGCAGTG TGGCTATTCC TAACCGCCCT TCTTCTGCCT TCCTCGCTTT GCCTGCTCCT 
CCCGGAGCAA TGTCATTGCC TCTGAACGGG GATGACCCCT TCGCAGCATC TGCTGTTGTT 
CCTCCTCCTG CGTATGTGCA GATGGCAGAT CTGGACACGA AGCAACAACT CTTATCTCAA 
GAACAAATCA TGTGGCAACG ATACCAGATG GAGGGTATGC GGGGCGAGGC GACTTTCCAG 
AAAGTGTTGA ACAATCCCTA CGTTTGCATG CCAGGTCCTG GCCCAATGAT GCCACCCTAC 
CAAAACCCTT ACACTGTTGG CATGCCCGTG GGATACCAAA CACCGTATTA A