TSC2 cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene
Clones
gRNAs

Summary

Gene Symbol	TSC2
Entrez Gene ID	100441126
Full Name	TSC complex subunit 2
Gene Type	protein-coding
Organism	Pongo abelii(Sumatran orangutan)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024234089.1	XP_024089857.1	tuberin isoform X4
XM_024234086.1	XP_024089854.1	tuberin isoform X1
XM_009250325.1	XP_009248600.1	LOW QUALITY PROTEIN: tuberin
XM_024234085.1	XP_024089853.1	tuberin isoform X1
XM_024234088.1	XP_024089856.1	tuberin isoform X3
XM_024234087.1	XP_024089855.1	tuberin isoform X2

Pathways from BioSystems
Gene Ontology

The following TSC2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSC2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa171438	XM_024234086.1 Latest version!	Pongo abelii TSC complex subunit 2 (TSC2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa171438	XM_024234085.1 Latest version!	Pongo abelii TSC complex subunit 2 (TSC2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa171439	XM_024234087.1 Latest version!	Pongo abelii TSC complex subunit 2 (TSC2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa171440	XM_024234088.1 Latest version!	Pongo abelii TSC complex subunit 2 (TSC2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa171441	XM_024234089.1 Latest version!	Pongo abelii TSC complex subunit 2 (TSC2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa28464	XM_009250325.1 Latest version!	Pongo abelii tuberous sclerosis 2 (TSC2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TSC2 ( XM_024234086.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024089854.1 Pongo abelii TSC complex subunit 2 (TSC2), transcript variant X2, mRNA.

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CloneID	OSa171438
Clone ID Related Accession (Same CDS sequence)	XM_024234086.1 , XM_024234085.1
Accession Version	XM_024234086.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036919.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT TGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTTTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CACTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAATG AAGACCTTCA CGAAAGGCTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTCTGCGT CCGGACCGCA TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACTCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ATAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTGAGGA ACTCGCCGAC ATCTGTGCTA 
CCATCATTTT ACCAGGCCAT GGTATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTACAGG AAGGAGCTCC AGGTGGTGAC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCGTTG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCGGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGGCGGCCCG CTCCCTCTCC 
CCACCCCCAG AGCTGGAAGA AAGGGATGCG GCCGCACACT CAGCCTCCTT GGAGGATGTG 
AAGACAGCTG TCCTGGGTCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGCGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATTGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTCGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCTC AGGGCCGCCT GGCCCGGCGC CTGCAGGTCC CGCCGTGCGG 
CTGGGTTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CCGAGTCCCT GCGCTACAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACCTGCACC TGGCTGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CCGTCCTGGT GGTGAAGCTC ACACACATCT CGGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG 
GGGTCTGCAG ACGAGAACTC CGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG 
GAGCTCACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT 
GTCCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTGGCGG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA 
CTAGGCCTGG ACTCGGGGGA GTTGCAGTCC GGCCCAGAGT CGAGCTCCAG CCCCGGCGTG 
CATGTGAGAC AGACAAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC 
GCCCTGGACA TGCCGGCCTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT 
GCACCAGCCG CGAAGCCTGA GAAGGCGTCA GCTGGCACCC GGGTTCCTGT GCAGGAGAAG 
ACGAACTTGG CGGCCTACGT ACCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCT 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAACG CCCTCATGGC GGCTGAGCGC 
TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCATTGT CGGTGCCGGC GGCCAGCACG 
GCCAGACCCC CTCCTCTGCC TCGCTCCAAC ACAGACTCCG CTGCGGTCGT GGAGGAGGGA 
AGTCCTGGCG AGGTTCCTGT GCCAGTGGAG CCCCCAGGCT TGGAGGACTT TGAGGCAGCG 
CTAGGCATGG ACAGGCGCAC GGATGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG 
GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GCCAGGGGCA TCCCCATCGA GCGAGCCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC 
AAGTCCAGCT CCTCTCCCGA GCTGCAGACC CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG 
GACGGGGAAA GTGCTGCCTG GTCAGCCTCG GGCGAAGACA GTCGGGGCCA GCCGGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC 
TCCGACTCGG CCCCATCACG GAGGGGCAAG AGGGTAGAGA GGGATGCCTT TAAGAGCAGA 
GCCACAGCCT CCAATGCAGA GAAAGTGCCG GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AACGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTGAAAGACT GCCAGCCCGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAT 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCGACC 
CTGATGCCCA CCAAGGACGT GGACAGGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAT 
GACTTTGTGT CCATTGTCTA CAATGACTCC GGCGAGGACT TTAAGCTCGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTATGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCT TGCACGCAAA TATGGCCTCA 
CAGGTGCACC ATAGCCGCTC CAACCCCACC GACATCTACC CCTCCAAGTG GATTGCCCGG 
CTCCGCCACA TCAAGAGGCT CCGCCAGCGG ATCTGTGAGG AAGCCGCCTA CTCCAACCCC 
AGCCTGCCTC TGGTGCACCC CCCGTCCCAT ACCAAAGCCC CTGCACAGAC TCCAGCCGAG 
CCCACACCTG GCTACGAGAT GGGCCAGCGG AAGCGCCTCG TCTCCTCCGT GGAGGACTTC 
ACCGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024089854.1
CDS	169..5523
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMVCPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTVVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVAARSLSPPPELEERDAAAHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPSGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDMPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTARPPPLPRSNTDSAAVVEEGSPGEVPVPVEPPGLEDFEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVARGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDRHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKAPAQTPAEPTPGYEMGQRKRLVSSVEDFTEFV

Target ORF information:

RefSeq Version	XM_024234086.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii TSC complex subunit 2 (TSC2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024234086.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT TGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTTTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CACTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAATG AAGACCTTCA CGAAAGGCTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTCTGCGT CCGGACCGCA TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACTCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ATAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTGAGGA ACTCGCCGAC ATCTGTGCTA 
CCATCATTTT ACCAGGCCAT GGTATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTACAGG AAGGAGCTCC AGGTGGTGAC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCGTTG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCGGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGGCGGCCCG CTCCCTCTCC 
CCACCCCCAG AGCTGGAAGA AAGGGATGCG GCCGCACACT CAGCCTCCTT GGAGGATGTG 
AAGACAGCTG TCCTGGGTCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGCGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATTGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTCGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCTC AGGGCCGCCT GGCCCGGCGC CTGCAGGTCC CGCCGTGCGG 
CTGGGTTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CCGAGTCCCT GCGCTACAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACCTGCACC TGGCTGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CCGTCCTGGT GGTGAAGCTC ACACACATCT CGGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG 
GGGTCTGCAG ACGAGAACTC CGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG 
GAGCTCACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT 
GTCCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTGGCGG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA 
CTAGGCCTGG ACTCGGGGGA GTTGCAGTCC GGCCCAGAGT CGAGCTCCAG CCCCGGCGTG 
CATGTGAGAC AGACAAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC 
GCCCTGGACA TGCCGGCCTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT 
GCACCAGCCG CGAAGCCTGA GAAGGCGTCA GCTGGCACCC GGGTTCCTGT GCAGGAGAAG 
ACGAACTTGG CGGCCTACGT ACCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCT 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAACG CCCTCATGGC GGCTGAGCGC 
TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCATTGT CGGTGCCGGC GGCCAGCACG 
GCCAGACCCC CTCCTCTGCC TCGCTCCAAC ACAGACTCCG CTGCGGTCGT GGAGGAGGGA 
AGTCCTGGCG AGGTTCCTGT GCCAGTGGAG CCCCCAGGCT TGGAGGACTT TGAGGCAGCG 
CTAGGCATGG ACAGGCGCAC GGATGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG 
GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GCCAGGGGCA TCCCCATCGA GCGAGCCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC 
AAGTCCAGCT CCTCTCCCGA GCTGCAGACC CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG 
GACGGGGAAA GTGCTGCCTG GTCAGCCTCG GGCGAAGACA GTCGGGGCCA GCCGGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC 
TCCGACTCGG CCCCATCACG GAGGGGCAAG AGGGTAGAGA GGGATGCCTT TAAGAGCAGA 
GCCACAGCCT CCAATGCAGA GAAAGTGCCG GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AACGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTGAAAGACT GCCAGCCCGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAT 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCGACC 
CTGATGCCCA CCAAGGACGT GGACAGGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAT 
GACTTTGTGT CCATTGTCTA CAATGACTCC GGCGAGGACT TTAAGCTCGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTATGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCT TGCACGCAAA TATGGCCTCA 
CAGGTGCACC ATAGCCGCTC CAACCCCACC GACATCTACC CCTCCAAGTG GATTGCCCGG 
CTCCGCCACA TCAAGAGGCT CCGCCAGCGG ATCTGTGAGG AAGCCGCCTA CTCCAACCCC 
AGCCTGCCTC TGGTGCACCC CCCGTCCCAT ACCAAAGCCC CTGCACAGAC TCCAGCCGAG 
CCCACACCTG GCTACGAGAT GGGCCAGCGG AAGCGCCTCG TCTCCTCCGT GGAGGACTTC 
ACCGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_024234085.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024089853.1 Pongo abelii TSC complex subunit 2 (TSC2), transcript variant X1, mRNA.

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CloneID	OSa171438
Clone ID Related Accession (Same CDS sequence)	XM_024234086.1 , XM_024234085.1
Accession Version	XM_024234085.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	tuberin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036919.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT TGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTTTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CACTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAATG AAGACCTTCA CGAAAGGCTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTCTGCGT CCGGACCGCA TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACTCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ATAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTGAGGA ACTCGCCGAC ATCTGTGCTA 
CCATCATTTT ACCAGGCCAT GGTATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTACAGG AAGGAGCTCC AGGTGGTGAC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCGTTG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCGGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGGCGGCCCG CTCCCTCTCC 
CCACCCCCAG AGCTGGAAGA AAGGGATGCG GCCGCACACT CAGCCTCCTT GGAGGATGTG 
AAGACAGCTG TCCTGGGTCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGCGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATTGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTCGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCTC AGGGCCGCCT GGCCCGGCGC CTGCAGGTCC CGCCGTGCGG 
CTGGGTTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CCGAGTCCCT GCGCTACAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACCTGCACC TGGCTGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CCGTCCTGGT GGTGAAGCTC ACACACATCT CGGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG 
GGGTCTGCAG ACGAGAACTC CGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG 
GAGCTCACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT 
GTCCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTGGCGG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA 
CTAGGCCTGG ACTCGGGGGA GTTGCAGTCC GGCCCAGAGT CGAGCTCCAG CCCCGGCGTG 
CATGTGAGAC AGACAAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC 
GCCCTGGACA TGCCGGCCTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT 
GCACCAGCCG CGAAGCCTGA GAAGGCGTCA GCTGGCACCC GGGTTCCTGT GCAGGAGAAG 
ACGAACTTGG CGGCCTACGT ACCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCT 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAACG CCCTCATGGC GGCTGAGCGC 
TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCATTGT CGGTGCCGGC GGCCAGCACG 
GCCAGACCCC CTCCTCTGCC TCGCTCCAAC ACAGACTCCG CTGCGGTCGT GGAGGAGGGA 
AGTCCTGGCG AGGTTCCTGT GCCAGTGGAG CCCCCAGGCT TGGAGGACTT TGAGGCAGCG 
CTAGGCATGG ACAGGCGCAC GGATGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG 
GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GCCAGGGGCA TCCCCATCGA GCGAGCCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC 
AAGTCCAGCT CCTCTCCCGA GCTGCAGACC CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG 
GACGGGGAAA GTGCTGCCTG GTCAGCCTCG GGCGAAGACA GTCGGGGCCA GCCGGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC 
TCCGACTCGG CCCCATCACG GAGGGGCAAG AGGGTAGAGA GGGATGCCTT TAAGAGCAGA 
GCCACAGCCT CCAATGCAGA GAAAGTGCCG GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AACGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTGAAAGACT GCCAGCCCGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAT 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCGACC 
CTGATGCCCA CCAAGGACGT GGACAGGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAT 
GACTTTGTGT CCATTGTCTA CAATGACTCC GGCGAGGACT TTAAGCTCGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTATGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCT TGCACGCAAA TATGGCCTCA 
CAGGTGCACC ATAGCCGCTC CAACCCCACC GACATCTACC CCTCCAAGTG GATTGCCCGG 
CTCCGCCACA TCAAGAGGCT CCGCCAGCGG ATCTGTGAGG AAGCCGCCTA CTCCAACCCC 
AGCCTGCCTC TGGTGCACCC CCCGTCCCAT ACCAAAGCCC CTGCACAGAC TCCAGCCGAG 
CCCACACCTG GCTACGAGAT GGGCCAGCGG AAGCGCCTCG TCTCCTCCGT GGAGGACTTC 
ACCGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024089853.1
CDS	194..5548
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMVCPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTVVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVAARSLSPPPELEERDAAAHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPSGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDMPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTARPPPLPRSNTDSAAVVEEGSPGEVPVPVEPPGLEDFEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVARGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDRHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKAPAQTPAEPTPGYEMGQRKRLVSSVEDFTEFV

Target ORF information:

RefSeq Version	XM_024234085.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii TSC complex subunit 2 (TSC2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024234085.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT TGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTTTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CACTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAATG AAGACCTTCA CGAAAGGCTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTCTGCGT CCGGACCGCA TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACTCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ATAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTGAGGA ACTCGCCGAC ATCTGTGCTA 
CCATCATTTT ACCAGGCCAT GGTATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTACAGG AAGGAGCTCC AGGTGGTGAC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCGTTG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCGGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGGCGGCCCG CTCCCTCTCC 
CCACCCCCAG AGCTGGAAGA AAGGGATGCG GCCGCACACT CAGCCTCCTT GGAGGATGTG 
AAGACAGCTG TCCTGGGTCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGCGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATTGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTCGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCTC AGGGCCGCCT GGCCCGGCGC CTGCAGGTCC CGCCGTGCGG 
CTGGGTTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CCGAGTCCCT GCGCTACAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACCTGCACC TGGCTGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CCGTCCTGGT GGTGAAGCTC ACACACATCT CGGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG 
GGGTCTGCAG ACGAGAACTC CGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG 
GAGCTCACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT 
GTCCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTGGCGG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA 
CTAGGCCTGG ACTCGGGGGA GTTGCAGTCC GGCCCAGAGT CGAGCTCCAG CCCCGGCGTG 
CATGTGAGAC AGACAAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGTTCC ATGTCGGGGG GCCATGGTCT TCGAGTTGGC 
GCCCTGGACA TGCCGGCCTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT 
GCACCAGCCG CGAAGCCTGA GAAGGCGTCA GCTGGCACCC GGGTTCCTGT GCAGGAGAAG 
ACGAACTTGG CGGCCTACGT ACCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCT 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAACG CCCTCATGGC GGCTGAGCGC 
TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCATTGT CGGTGCCGGC GGCCAGCACG 
GCCAGACCCC CTCCTCTGCC TCGCTCCAAC ACAGACTCCG CTGCGGTCGT GGAGGAGGGA 
AGTCCTGGCG AGGTTCCTGT GCCAGTGGAG CCCCCAGGCT TGGAGGACTT TGAGGCAGCG 
CTAGGCATGG ACAGGCGCAC GGATGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG 
GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GCCAGGGGCA TCCCCATCGA GCGAGCCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC 
AAGTCCAGCT CCTCTCCCGA GCTGCAGACC CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG 
GACGGGGAAA GTGCTGCCTG GTCAGCCTCG GGCGAAGACA GTCGGGGCCA GCCGGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC 
TCCGACTCGG CCCCATCACG GAGGGGCAAG AGGGTAGAGA GGGATGCCTT TAAGAGCAGA 
GCCACAGCCT CCAATGCAGA GAAAGTGCCG GGCATCAACC CCAGTTTCGT GTTCCTGCAG 
CTCTACCATT CCCCCTTCTT TGGCGACGAG TCAAACAAGC CAATCCTGCT GCCCAATGAG 
TCACAGTCCT TTGAGCGGTC GGTGCAGCTC CTCGACCAGA TCCCATCATA CGACACCCAC 
AAGATCGCCG TCCTGTATGT TGGAGAAGGC CAGAGCAACA GCGAGCTCGC CATCCTGTCC 
AACGAGCATG GCTCCTACAG GTACACGGAG TTCCTGACGG GCCTGGGCCG GCTCATCGAG 
CTGAAAGACT GCCAGCCCGA CAAGGTGTAC CTGGGAGGCC TGGACGTGTG TGGTGAGGAT 
GGCCAGTTCA CCTACTGCTG GCACGATGAC ATCATGCAAG CCGTCTTCCA CATCGCGACC 
CTGATGCCCA CCAAGGACGT GGACAGGCAC CGCTGCGACA AGAAGCGCCA CCTGGGCAAT 
GACTTTGTGT CCATTGTCTA CAATGACTCC GGCGAGGACT TTAAGCTCGG CACCATCAAG 
GGCCAGTTCA ACTTTGTCCA CGTGATCGTC ACCCCGCTGG ACTATGAGTG CAACCTGGTG 
TCCCTGCAGT GCAGGAAAGA CATGGAGGGC CTTGTGGACA CCAGCGTGGC CAAGATCGTG 
TCTGACCGCA ACCTGCCCTT CGTGGCCCGC CAGATGGCCT TGCACGCAAA TATGGCCTCA 
CAGGTGCACC ATAGCCGCTC CAACCCCACC GACATCTACC CCTCCAAGTG GATTGCCCGG 
CTCCGCCACA TCAAGAGGCT CCGCCAGCGG ATCTGTGAGG AAGCCGCCTA CTCCAACCCC 
AGCCTGCCTC TGGTGCACCC CCCGTCCCAT ACCAAAGCCC CTGCACAGAC TCCAGCCGAG 
CCCACACCTG GCTACGAGAT GGGCCAGCGG AAGCGCCTCG TCTCCTCCGT GGAGGACTTC 
ACCGAGTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_024234087.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024089855.1 Pongo abelii TSC complex subunit 2 (TSC2), transcript variant X3, mRNA.

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CloneID	OSa171439
Clone ID Related Accession (Same CDS sequence)	XM_024234087.1
Accession Version	XM_024234087.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	tuberin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036919.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT TGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTTTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CACTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAATG AAGACCTTCA CGAAAGGCTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTCTGCGT CCGGACCGCA TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACTCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ATAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTGAGGA ACTCGCCGAC ATCTGTGCTA 
CCATCATTTT ACCAGGCCAT GGTATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTACAGG AAGGAGCTCC AGGTGGTGAC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCGTTG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCGGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGGCGGCCCG CTCCCTCTCC 
CCACCCCCAG AGCTGGAAGA AAGGGATGCG GCCGCACACT CAGCCTCCTT GGAGGATGTG 
AAGACAGCTG TCCTGGGTCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGCGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATTGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTCGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCTC AGGGCCGCCT GGCCCGGCGC CTGCAGGTCC CGCCGTGCGG 
CTGGGTTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CCGAGTCCCT GCGCTACAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACCTGCACC TGGCTGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CCGTCCTGGT GGTGAAGCTC ACACACATCT CGGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGCAG GATACAGACG TCCCTCACCA GTGCCAGCTT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA ACCTCCACCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCTCGATAC GTCTTCTCCA ACTTCACGGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACAAGCGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGTTGCAGTC CGGCCCAGAG TCGAGCTCCA GCCCCGGCGT GCATGTGAGA 
CAGACAAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC CATGTCGGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAC 
ATGCCGGCCT CCCAGTTCCT GGGCAGTGCC ACTTCTCCAG GACCACGGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCGTC AGCTGGCACC CGGGTTCCTG TGCAGGAGAA GACGAACTTG 
GCGGCCTACG TACCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC TTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CGGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCATTG TCGGTGCCGG CGGCCAGCAC GGCCAGACCC 
CCTCCTCTGC CTCGCTCCAA CACAGACTCC GCTGCGGTCG TGGAGGAGGG AAGTCCTGGC 
GAGGTTCCTG TGCCAGTGGA GCCCCCAGGC TTGGAGGACT TTGAGGCAGC GCTAGGCATG 
GACAGGCGCA CGGATGCCTA CAGCAGGTCG TCCTCAGTCT CCAGCCAGGA GGAGAAGTCG 
CTCCACGCGG AGGAGCTGGT TGCCAGGGGC ATCCCCATCG AGCGAGCCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCGC AGCCCCTGAG CAAGTCCAGC 
TCCTCTCCCG AGCTGCAGAC CCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCCGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGGTCACAGT CAGGGACCCT GGACGGGGAA 
AGTGCTGCCT GGTCAGCCTC GGGCGAAGAC AGTCGGGGCC AGCCGGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCGCC CAGTGGCCTC CGGCCCCGAG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GGAGGGGCAA GAGGGTAGAG AGGGATGCCT TTAAGAGCAG AGCCACAGCC 
TCCAATGCAG AGAAAGTGCC GGGCATCAAC CCCAGTTTCG TGTTCCTGCA GCTCTACCAT 
TCCCCCTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC 
TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC 
GTCCTGTATG TTGGAGAAGG CCAGAGCAAC AGCGAGCTCG CCATCCTGTC CAACGAGCAT 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTGAAAGAC 
TGCCAGCCCG ACAAGGTGTA CCTGGGAGGC CTGGACGTGT GTGGTGAGGA TGGCCAGTTC 
ACCTACTGCT GGCACGATGA CATCATGCAA GCCGTCTTCC ACATCGCGAC CCTGATGCCC 
ACCAAGGACG TGGACAGGCA CCGCTGCGAC AAGAAGCGCC ACCTGGGCAA TGACTTTGTG 
TCCATTGTCT ACAATGACTC CGGCGAGGAC TTTAAGCTCG GCACCATCAA GGGCCAGTTC 
AACTTTGTCC ACGTGATCGT CACCCCGCTG GACTATGAGT GCAACCTGGT GTCCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC 
AACCTGCCCT TCGTGGCCCG CCAGATGGCC TTGCACGCAA ATATGGCCTC ACAGGTGCAC 
CATAGCCGCT CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC 
ATCAAGAGGC TCCGCCAGCG GATCTGTGAG GAAGCCGCCT ACTCCAACCC CAGCCTGCCT 
CTGGTGCACC CCCCGTCCCA TACCAAAGCC CCTGCACAGA CTCCAGCCGA GCCCACACCT 
GGCTACGAGA TGGGCCAGCG GAAGCGCCTC GTCTCCTCCG TGGAGGACTT CACCGAGTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024089855.1
CDS	194..5419
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMVCPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTVVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVAARSLSPPPELEERDAAAHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPSGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDMPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTARPPPLPRSNTDSAAVVEEGSPGEVPVPVEPPGLEDFEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVARGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDRHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKAPAQTPAEPTPGYEMGQRKRLVSSVEDFTEFV

Target ORF information:

RefSeq Version	XM_024234087.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii TSC complex subunit 2 (TSC2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024234087.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT TGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTTTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CACTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAATG AAGACCTTCA CGAAAGGCTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTCTGCGT CCGGACCGCA TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACTCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ATAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTGAGGA ACTCGCCGAC ATCTGTGCTA 
CCATCATTTT ACCAGGCCAT GGTATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTACAGG AAGGAGCTCC AGGTGGTGAC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCGTTG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCGGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGGCGGCCCG CTCCCTCTCC 
CCACCCCCAG AGCTGGAAGA AAGGGATGCG GCCGCACACT CAGCCTCCTT GGAGGATGTG 
AAGACAGCTG TCCTGGGTCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGCGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATTGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTCGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCTC AGGGCCGCCT GGCCCGGCGC CTGCAGGTCC CGCCGTGCGG 
CTGGGTTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CCGAGTCCCT GCGCTACAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACCTGCACC TGGCTGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CCGTCCTGGT GGTGAAGCTC ACACACATCT CGGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGCAG GATACAGACG TCCCTCACCA GTGCCAGCTT GGGGTCTGCA 
GACGAGAACT CCGTGGCCCA GGCTGACGAT AGCCTGAAAA ACCTCCACCT GGAGCTCACG 
GAAACCTGTC TGGACATGAT GGCTCGATAC GTCTTCTCCA ACTTCACGGC TGTCCCGAAG 
AGGTCTCCTG TGGGCGAGTT CCTCCTGGCG GGTGGCAGGA CCAAAACCTG GCTGGTTGGG 
AACAAGCTTG TCACTGTGAC GACAAGCGTG GGAACCGGGA CCCGGTCGTT ACTAGGCCTG 
GACTCGGGGG AGTTGCAGTC CGGCCCAGAG TCGAGCTCCA GCCCCGGCGT GCATGTGAGA 
CAGACAAAGG AGGCGCCGGC CAAGCTGGAG TCCCAGGCTG GGCAGCAGGT GTCCCGTGGG 
GCCCGGGACC GGGTCCGTTC CATGTCGGGG GGCCATGGTC TTCGAGTTGG CGCCCTGGAC 
ATGCCGGCCT CCCAGTTCCT GGGCAGTGCC ACTTCTCCAG GACCACGGAC TGCACCAGCC 
GCGAAGCCTG AGAAGGCGTC AGCTGGCACC CGGGTTCCTG TGCAGGAGAA GACGAACTTG 
GCGGCCTACG TACCCCTGCT GACCCAGGGC TGGGCGGAGA TCCTGGTCCG GAGGCCCACA 
GGGAACACCA GCTGGCTGAT GAGCCTGGAG AACCCGCTCA GCCCTTTCTC TTCGGACATC 
AACAACATGC CCCTGCAGGA GCTGTCCAAC GCCCTCATGG CGGCTGAGCG CTTCAAGGAG 
CACCGGGACA CAGCCCTGTA CAAGTCATTG TCGGTGCCGG CGGCCAGCAC GGCCAGACCC 
CCTCCTCTGC CTCGCTCCAA CACAGACTCC GCTGCGGTCG TGGAGGAGGG AAGTCCTGGC 
GAGGTTCCTG TGCCAGTGGA GCCCCCAGGC TTGGAGGACT TTGAGGCAGC GCTAGGCATG 
GACAGGCGCA CGGATGCCTA CAGCAGGTCG TCCTCAGTCT CCAGCCAGGA GGAGAAGTCG 
CTCCACGCGG AGGAGCTGGT TGCCAGGGGC ATCCCCATCG AGCGAGCCGT CTCCTCGGAG 
GGTGGCCGGC CCTCTGTGGA CCTCTCCTTC CAGCCCTCGC AGCCCCTGAG CAAGTCCAGC 
TCCTCTCCCG AGCTGCAGAC CCTGCAGGAC ATCCTCGGGG ACCCTGGGGA CAAGGCCGAC 
GTGGGCCGGC TGAGCCCTGA GGTTAAGGCC CGGTCACAGT CAGGGACCCT GGACGGGGAA 
AGTGCTGCCT GGTCAGCCTC GGGCGAAGAC AGTCGGGGCC AGCCGGAGGG TCCCTTGCCT 
TCCAGCTCCC CCCGCTCGCC CAGTGGCCTC CGGCCCCGAG GTTACACCAT CTCCGACTCG 
GCCCCATCAC GGAGGGGCAA GAGGGTAGAG AGGGATGCCT TTAAGAGCAG AGCCACAGCC 
TCCAATGCAG AGAAAGTGCC GGGCATCAAC CCCAGTTTCG TGTTCCTGCA GCTCTACCAT 
TCCCCCTTCT TTGGCGACGA GTCAAACAAG CCAATCCTGC TGCCCAATGA GTCACAGTCC 
TTTGAGCGGT CGGTGCAGCT CCTCGACCAG ATCCCATCAT ACGACACCCA CAAGATCGCC 
GTCCTGTATG TTGGAGAAGG CCAGAGCAAC AGCGAGCTCG CCATCCTGTC CAACGAGCAT 
GGCTCCTACA GGTACACGGA GTTCCTGACG GGCCTGGGCC GGCTCATCGA GCTGAAAGAC 
TGCCAGCCCG ACAAGGTGTA CCTGGGAGGC CTGGACGTGT GTGGTGAGGA TGGCCAGTTC 
ACCTACTGCT GGCACGATGA CATCATGCAA GCCGTCTTCC ACATCGCGAC CCTGATGCCC 
ACCAAGGACG TGGACAGGCA CCGCTGCGAC AAGAAGCGCC ACCTGGGCAA TGACTTTGTG 
TCCATTGTCT ACAATGACTC CGGCGAGGAC TTTAAGCTCG GCACCATCAA GGGCCAGTTC 
AACTTTGTCC ACGTGATCGT CACCCCGCTG GACTATGAGT GCAACCTGGT GTCCCTGCAG 
TGCAGGAAAG ACATGGAGGG CCTTGTGGAC ACCAGCGTGG CCAAGATCGT GTCTGACCGC 
AACCTGCCCT TCGTGGCCCG CCAGATGGCC TTGCACGCAA ATATGGCCTC ACAGGTGCAC 
CATAGCCGCT CCAACCCCAC CGACATCTAC CCCTCCAAGT GGATTGCCCG GCTCCGCCAC 
ATCAAGAGGC TCCGCCAGCG GATCTGTGAG GAAGCCGCCT ACTCCAACCC CAGCCTGCCT 
CTGGTGCACC CCCCGTCCCA TACCAAAGCC CCTGCACAGA CTCCAGCCGA GCCCACACCT 
GGCTACGAGA TGGGCCAGCG GAAGCGCCTC GTCTCCTCCG TGGAGGACTT CACCGAGTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_024234088.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024089856.1 Pongo abelii TSC complex subunit 2 (TSC2), transcript variant X4, mRNA.

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CloneID	OSa171440
Clone ID Related Accession (Same CDS sequence)	XM_024234088.1
Accession Version	XM_024234088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	tuberin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036919.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT TGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTTTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CACTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAATG AAGACCTTCA CGAAAGGCTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTCTGCGT CCGGACCGCA TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACTCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ATAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTGAGGA ACTCGCCGAC ATCTGTGCTA 
CCATCATTTT ACCAGGCCAT GGTATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTACAGG AAGGAGCTCC AGGTGGTGAC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCGTTG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCGGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGGCGGCCCG CTCCCTCTCC 
CCACCCCCAG AGCTGGAAGA AAGGGATGCG GCCGCACACT CAGCCTCCTT GGAGGATGTG 
AAGACAGCTG TCCTGGGTCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGCGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATTGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTCGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCTC AGGGCCGCCT GGCCCGGCGC CTGCAGGTCC CGCCGTGCGG 
CTGGGTTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CCGAGTCCCT GCGCTACAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACCTGCACC TGGCTGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CCGTCCTGGT GGTGAAGCTC ACACACATCT CGGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGGAT ACAGACGTCC CTCACCAGTG CCAGCTTGGG GTCTGCAGAC 
GAGAACTCCG TGGCCCAGGC TGACGATAGC CTGAAAAACC TCCACCTGGA GCTCACGGAA 
ACCTGTCTGG ACATGATGGC TCGATACGTC TTCTCCAACT TCACGGCTGT CCCGAAGAGG 
TCTCCTGTGG GCGAGTTCCT CCTGGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC AAGCGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC 
TCGGGGGAGT TGCAGTCCGG CCCAGAGTCG AGCTCCAGCC CCGGCGTGCA TGTGAGACAG 
ACAAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGACCGGG TCCGTTCCAT GTCGGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGACATG 
CCGGCCTCCC AGTTCCTGGG CAGTGCCACT TCTCCAGGAC CACGGACTGC ACCAGCCGCG 
AAGCCTGAGA AGGCGTCAGC TGGCACCCGG GTTCCTGTGC AGGAGAAGAC GAACTTGGCG 
GCCTACGTAC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCTTC GGACATCAAC 
AACATGCCCC TGCAGGAGCT GTCCAACGCC CTCATGGCGG CTGAGCGCTT CAAGGAGCAC 
CGGGACACAG CCCTGTACAA GTCATTGTCG GTGCCGGCGG CCAGCACGGC CAGACCCCCT 
CCTCTGCCTC GCTCCAACAC AGACTCCGCT GCGGTCGTGG AGGAGGGAAG TCCTGGCGAG 
GTTCCTGTGC CAGTGGAGCC CCCAGGCTTG GAGGACTTTG AGGCAGCGCT AGGCATGGAC 
AGGCGCACGG ATGCCTACAG CAGGTCGTCC TCAGTCTCCA GCCAGGAGGA GAAGTCGCTC 
CACGCGGAGG AGCTGGTTGC CAGGGGCATC CCCATCGAGC GAGCCGTCTC CTCGGAGGGT 
GGCCGGCCCT CTGTGGACCT CTCCTTCCAG CCCTCGCAGC CCCTGAGCAA GTCCAGCTCC 
TCTCCCGAGC TGCAGACCCT GCAGGACATC CTCGGGGACC CTGGGGACAA GGCCGACGTG 
GGCCGGCTGA GCCCTGAGGT TAAGGCCCGG TCACAGTCAG GGACCCTGGA CGGGGAAAGT 
GCTGCCTGGT CAGCCTCGGG CGAAGACAGT CGGGGCCAGC CGGAGGGTCC CTTGCCTTCC 
AGCTCCCCCC GCTCGCCCAG TGGCCTCCGG CCCCGAGGTT ACACCATCTC CGACTCGGCC 
CCATCACGGA GGGGCAAGAG GGTAGAGAGG GATGCCTTTA AGAGCAGAGC CACAGCCTCC 
AATGCAGAGA AAGTGCCGGG CATCAACCCC AGTTTCGTGT TCCTGCAGCT CTACCATTCC 
CCCTTCTTTG GCGACGAGTC AAACAAGCCA ATCCTGCTGC CCAATGAGTC ACAGTCCTTT 
GAGCGGTCGG TGCAGCTCCT CGACCAGATC CCATCATACG ACACCCACAA GATCGCCGTC 
CTGTATGTTG GAGAAGGCCA GAGCAACAGC GAGCTCGCCA TCCTGTCCAA CGAGCATGGC 
TCCTACAGGT ACACGGAGTT CCTGACGGGC CTGGGCCGGC TCATCGAGCT GAAAGACTGC 
CAGCCCGACA AGGTGTACCT GGGAGGCCTG GACGTGTGTG GTGAGGATGG CCAGTTCACC 
TACTGCTGGC ACGATGACAT CATGCAAGCC GTCTTCCACA TCGCGACCCT GATGCCCACC 
AAGGACGTGG ACAGGCACCG CTGCGACAAG AAGCGCCACC TGGGCAATGA CTTTGTGTCC 
ATTGTCTACA ATGACTCCGG CGAGGACTTT AAGCTCGGCA CCATCAAGGG CCAGTTCAAC 
TTTGTCCACG TGATCGTCAC CCCGCTGGAC TATGAGTGCA ACCTGGTGTC CCTGCAGTGC 
AGGAAAGACA TGGAGGGCCT TGTGGACACC AGCGTGGCCA AGATCGTGTC TGACCGCAAC 
CTGCCCTTCG TGGCCCGCCA GATGGCCTTG CACGCAAATA TGGCCTCACA GGTGCACCAT 
AGCCGCTCCA ACCCCACCGA CATCTACCCC TCCAAGTGGA TTGCCCGGCT CCGCCACATC 
AAGAGGCTCC GCCAGCGGAT CTGTGAGGAA GCCGCCTACT CCAACCCCAG CCTGCCTCTG 
GTGCACCCCC CGTCCCATAC CAAAGCCCCT GCACAGACTC CAGCCGAGCC CACACCTGGC 
TACGAGATGG GCCAGCGGAA GCGCCTCGTC TCCTCCGTGG AGGACTTCAC CGAGTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024089856.1
CDS	194..5416
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMVCPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTVVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVAARSLSPPPELEERDAAAHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPSGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDMPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTARPPPLPRSNTDSAAVVEEGSPGEVPVPVEPPGLEDFEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVARGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDRHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKAPAQTPAEPTPGYEMGQRKRLVSSVEDFTEFV

Target ORF information:

RefSeq Version	XM_024234088.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii TSC complex subunit 2 (TSC2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024234088.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT TGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTTTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CACTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGCG AGCGTTTGGG GGTCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAATG AAGACCTTCA CGAAAGGCTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTCTGCGT CCGGACCGCA TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACTCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ATAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGACGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTGAGGA ACTCGCCGAC ATCTGTGCTA 
CCATCATTTT ACCAGGCCAT GGTATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTACAGG AAGGAGCTCC AGGTGGTGAC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCGTTG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCGGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCC TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGGCGGCCCG CTCCCTCTCC 
CCACCCCCAG AGCTGGAAGA AAGGGATGCG GCCGCACACT CAGCCTCCTT GGAGGATGTG 
AAGACAGCTG TCCTGGGTCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGCGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATTGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTCGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCTC AGGGCCGCCT GGCCCGGCGC CTGCAGGTCC CGCCGTGCGG 
CTGGGTTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CCGAGTCCCT GCGCTACAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACCTGCACC TGGCTGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CCGTCCTGGT GGTGAAGCTC ACACACATCT CGGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGGAT ACAGACGTCC CTCACCAGTG CCAGCTTGGG GTCTGCAGAC 
GAGAACTCCG TGGCCCAGGC TGACGATAGC CTGAAAAACC TCCACCTGGA GCTCACGGAA 
ACCTGTCTGG ACATGATGGC TCGATACGTC TTCTCCAACT TCACGGCTGT CCCGAAGAGG 
TCTCCTGTGG GCGAGTTCCT CCTGGCGGGT GGCAGGACCA AAACCTGGCT GGTTGGGAAC 
AAGCTTGTCA CTGTGACGAC AAGCGTGGGA ACCGGGACCC GGTCGTTACT AGGCCTGGAC 
TCGGGGGAGT TGCAGTCCGG CCCAGAGTCG AGCTCCAGCC CCGGCGTGCA TGTGAGACAG 
ACAAAGGAGG CGCCGGCCAA GCTGGAGTCC CAGGCTGGGC AGCAGGTGTC CCGTGGGGCC 
CGGGACCGGG TCCGTTCCAT GTCGGGGGGC CATGGTCTTC GAGTTGGCGC CCTGGACATG 
CCGGCCTCCC AGTTCCTGGG CAGTGCCACT TCTCCAGGAC CACGGACTGC ACCAGCCGCG 
AAGCCTGAGA AGGCGTCAGC TGGCACCCGG GTTCCTGTGC AGGAGAAGAC GAACTTGGCG 
GCCTACGTAC CCCTGCTGAC CCAGGGCTGG GCGGAGATCC TGGTCCGGAG GCCCACAGGG 
AACACCAGCT GGCTGATGAG CCTGGAGAAC CCGCTCAGCC CTTTCTCTTC GGACATCAAC 
AACATGCCCC TGCAGGAGCT GTCCAACGCC CTCATGGCGG CTGAGCGCTT CAAGGAGCAC 
CGGGACACAG CCCTGTACAA GTCATTGTCG GTGCCGGCGG CCAGCACGGC CAGACCCCCT 
CCTCTGCCTC GCTCCAACAC AGACTCCGCT GCGGTCGTGG AGGAGGGAAG TCCTGGCGAG 
GTTCCTGTGC CAGTGGAGCC CCCAGGCTTG GAGGACTTTG AGGCAGCGCT AGGCATGGAC 
AGGCGCACGG ATGCCTACAG CAGGTCGTCC TCAGTCTCCA GCCAGGAGGA GAAGTCGCTC 
CACGCGGAGG AGCTGGTTGC CAGGGGCATC CCCATCGAGC GAGCCGTCTC CTCGGAGGGT 
GGCCGGCCCT CTGTGGACCT CTCCTTCCAG CCCTCGCAGC CCCTGAGCAA GTCCAGCTCC 
TCTCCCGAGC TGCAGACCCT GCAGGACATC CTCGGGGACC CTGGGGACAA GGCCGACGTG 
GGCCGGCTGA GCCCTGAGGT TAAGGCCCGG TCACAGTCAG GGACCCTGGA CGGGGAAAGT 
GCTGCCTGGT CAGCCTCGGG CGAAGACAGT CGGGGCCAGC CGGAGGGTCC CTTGCCTTCC 
AGCTCCCCCC GCTCGCCCAG TGGCCTCCGG CCCCGAGGTT ACACCATCTC CGACTCGGCC 
CCATCACGGA GGGGCAAGAG GGTAGAGAGG GATGCCTTTA AGAGCAGAGC CACAGCCTCC 
AATGCAGAGA AAGTGCCGGG CATCAACCCC AGTTTCGTGT TCCTGCAGCT CTACCATTCC 
CCCTTCTTTG GCGACGAGTC AAACAAGCCA ATCCTGCTGC CCAATGAGTC ACAGTCCTTT 
GAGCGGTCGG TGCAGCTCCT CGACCAGATC CCATCATACG ACACCCACAA GATCGCCGTC 
CTGTATGTTG GAGAAGGCCA GAGCAACAGC GAGCTCGCCA TCCTGTCCAA CGAGCATGGC 
TCCTACAGGT ACACGGAGTT CCTGACGGGC CTGGGCCGGC TCATCGAGCT GAAAGACTGC 
CAGCCCGACA AGGTGTACCT GGGAGGCCTG GACGTGTGTG GTGAGGATGG CCAGTTCACC 
TACTGCTGGC ACGATGACAT CATGCAAGCC GTCTTCCACA TCGCGACCCT GATGCCCACC 
AAGGACGTGG ACAGGCACCG CTGCGACAAG AAGCGCCACC TGGGCAATGA CTTTGTGTCC 
ATTGTCTACA ATGACTCCGG CGAGGACTTT AAGCTCGGCA CCATCAAGGG CCAGTTCAAC 
TTTGTCCACG TGATCGTCAC CCCGCTGGAC TATGAGTGCA ACCTGGTGTC CCTGCAGTGC 
AGGAAAGACA TGGAGGGCCT TGTGGACACC AGCGTGGCCA AGATCGTGTC TGACCGCAAC 
CTGCCCTTCG TGGCCCGCCA GATGGCCTTG CACGCAAATA TGGCCTCACA GGTGCACCAT 
AGCCGCTCCA ACCCCACCGA CATCTACCCC TCCAAGTGGA TTGCCCGGCT CCGCCACATC 
AAGAGGCTCC GCCAGCGGAT CTGTGAGGAA GCCGCCTACT CCAACCCCAG CCTGCCTCTG 
GTGCACCCCC CGTCCCATAC CAAAGCCCCT GCACAGACTC CAGCCGAGCC CACACCTGGC 
TACGAGATGG GCCAGCGGAA GCGCCTCGTC TCCTCCGTGG AGGACTTCAC CGAGTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_024234089.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024089857.1 Pongo abelii TSC complex subunit 2 (TSC2), transcript variant X5, mRNA.

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CloneID	OSa171441
Clone ID Related Accession (Same CDS sequence)	XM_024234089.1
Accession Version	XM_024234089.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	tuberin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036919.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATGTG GCCTCAACAA TCGCATCCGG ATGATAGGGC AGATTTGTGA AGTTGCAAAA 
ACCAAGAAAT TTGAAGAGCA CGCAGTGGAA GCACTTTGGA AGGCGGTCGC GGATCTGTTG 
CAGCCGGAGC GGCCACTGGA GGCCCGGCAC GCGGTGCTGG CTCTGCTGAA GGCCATCGTG 
CAGGGACAGG GCGAGCGTTT GGGGGTCCTC AGAGCCCTCT TCTTTAAGGT CATCAAGGAT 
TACCCTTCCA ATGAAGACCT TCACGAAAGG CTGGAAGTTT TCAAGGCCCT CACAGACAAT 
GGGAGACACA TCACCTACTT GGAGGAAGAG CTGGCTGACT TTGTCCTGCA GTGGATGGAT 
GTTGGCTTGT CCTCGGAATT CCTTCTGGTG CTGGTGAACT TGGTCAAATT CAATAGCTGT 
TACCTCGACG AGTACATCGC AAGGATGGTT CAGATGATCT GTCTGCTCTG CGTCCGGACC 
GCATCCTCTG TGGACATAGA GGTCTCCCTG CAGGTGCTGG ACGCTGTGGT CTGCTACAAC 
TGCCTGCCGG CTGAGAGCCT CCCGCTGTTC ATCGTTACTC TCTGTCGCAC CATCAACGTC 
AAGGAGCTCT GCGAGCCTTG CTGGAAGCTG ATGCGGAACC TCCTTGGCAC CCACCTGGGC 
CACAGCGCCA TCTATAACAT GTGCCACCTC ATGGAGGACA GAGCCTACAT GGAGGACGCG 
CCCCTGCTGA GAGGAGCCGT GTTTTTTGTG GGCATGGCTC TCTGGGGAGC CCACCGGCTC 
TATTCTCTGA GGAACTCGCC GACATCTGTG CTACCATCAT TTTACCAGGC CATGGTATGT 
CCGAACGAGG TGGTGTCCTA TGAGATCGTC CTGTCCATCA CCAGGCTCAT CAAGAAGTAC 
AGGAAGGAGC TCCAGGTGGT GACGTGGGAC ATTCTGCTGA ACATCATCGA ACGGCTCCTT 
CAGCAGCTCC AGACCTTGGA CAGCCCGGAG CTCAGGACCG TTGTCCATGA CCTGTTGACC 
ACGGTGGAGG AGCTGTGTGA CCAGAACGAG TTCCACGGGT CTCAGGAGAG ATACTTTGAA 
CTGGTGGAGA GATGTGCGGA CCAGAGGCCG GAGTCCTCCC TCCTGAACCT GATCTCCTAT 
AGAGCGCAGT CCATCCACCC GGCCAAGGAC GGCTGGATTC AGAACCTGCA GGCGCTGATG 
GAGAGATTCT TCAGGAGCGA GTCCCGAGGC GCCGTGCGCA TCAAGGTGCT GGACGTGCTG 
TCCTTTGTGC TGCTCATCAA CAGGCAGTTC TATGAGGAGG AGCTGATTAA CTCAGTGGTC 
ATCTCGCAGC TCTCCCACAT CCCCGAGGAT AAAGACCACC AGGTCCGAAA GCTGGCCACC 
CAGTTGCTGG TGGACCTGGC AGAGGGCTGC CACACACACC ACTTCAACAG CCTGCTGGAC 
ATCATCGAGA AGGTGGCGGC CCGCTCCCTC TCCCCACCCC CAGAGCTGGA AGAAAGGGAT 
GCGGCCGCAC ACTCAGCCTC CTTGGAGGAT GTGAAGACAG CTGTCCTGGG TCTTCTGGTC 
ATCCTTCAGA CCAAGCTGTA CACCCTGCCT GCAAGCCACG CCACGCGCGT GTATGAGATG 
CTGGTCAGCC ACATTCAGCT CCACTACAAG CACAGCTACA CCCTGCCAAT TGCGAGCAGC 
ATCCGGCTGC AGGCCTTTGA CTTCCTGTTG CTGCTGCGGG CCGACTCACT GCACCGCCTC 
GGCCTGCCCA ACAAGGATGG AGTCGTGCGG TTCAGCCCCT ACTGCGTCTG CGACTACATG 
GAGCCAGAGA GAGGCTCTGA GAAGAAGACC AGCGGCCCCC TTTCTCCTCC CTCAGGGCCG 
CCTGGCCCGG CGCCTGCAGG TCCCGCCGTG CGGCTGGGTT CCCTGCCCTA CTCCCTGCTC 
TTCCGTGTCC TGCTGCAGTG CTTGAAGCAG GAGTCTGACT GGAAGGTGCT GAAGCTGGTT 
CTGGGCAGGC TGCCCGAGTC CCTGCGCTAC AAAGTGCTCA TCTTTACTTC CCCTTGCAGT 
GTGGACCAGC TGTGCTCTGC TCTCTGCTCC ATGCTTTCAG GCCCAAAGAC ACTGGAGCGG 
CTCCGAGGCG CCCCAGAAGG CTTCTCCAGA ACTGACCTGC ACCTGGCTGT GGTTCCAGTG 
CTGACAGCAT TAATCTCTTA CCATAACTAC CTGGACAAAA CCAAACAGCG CGAGATGGTC 
TACTGCCTGG AGCAGGGCCT CATCCACCGC TGTGCCAGCC AGTGCGTCGT GGCCTTGTCC 
ATCTGCAGCG TGGAGATGCC TGACATCATC ATCAAGGCGC TGCCCGTCCT GGTGGTGAAG 
CTCACACACA TCTCGGCCAC AGCCAGCATG GCCGTCCCAC TGCTGGAGTT CCTGTCCACT 
CTGGCCAGGC TGCCGCACCT CTACAGGAAC TTTGCCGCGG AGCAGTATGC CAGTGTGTTC 
GCCATCTCCC TGCCGTACAC CAACCCCTCC AAGTTTAATC AGTACATCGT GTGTCTGGCC 
CATCACGTCA TAGCCATGTG GTTCATCAGG TGCCGCCTGC CCTTCCGGAA GGATTTCGTC 
CCTTTCATCA CTAAGGGCCT GCGGTCCAAT GTCCTCTTGT CTTTTGATGA CACCCCCGAG 
AAGGACAGCT TCAGGGCCCG GAGTACTAGT CTCAACGAGA GACCCAAGAG TCTGAGGATA 
GCCAGACCCC CCAAACAAGG CTTGAATAAC TCTCCACCCG TGAAAGAATT CAAGGAGAGC 
TCTGCAGCCG AGGCCTTCCG GTGCCGCAGC ATCAGTGTGT CTGAACATGT GGTCCGCAGC 
AGGATACAGA CGTCCCTCAC CAGTGCCAGC TTGGGGTCTG CAGACGAGAA CTCCGTGGCC 
CAGGCTGACG ATAGCCTGAA AAACCTCCAC CTGGAGCTCA CGGAAACCTG TCTGGACATG 
ATGGCTCGAT ACGTCTTCTC CAACTTCACG GCTGTCCCGA AGAGGTCTCC TGTGGGCGAG 
TTCCTCCTGG CGGGTGGCAG GACCAAAACC TGGCTGGTTG GGAACAAGCT TGTCACTGTG 
ACGACAAGCG TGGGAACCGG GACCCGGTCG TTACTAGGCC TGGACTCGGG GGAGTTGCAG 
TCCGGCCCAG AGTCGAGCTC CAGCCCCGGC GTGCATGTGA GACAGACAAA GGAGGCGCCG 
GCCAAGCTGG AGTCCCAGGC TGGGCAGCAG GTGTCCCGTG GGGCCCGGGA CCGGGTCCGT 
TCCATGTCGG GGGGCCATGG TCTTCGAGTT GGCGCCCTGG ACATGCCGGC CTCCCAGTTC 
CTGGGCAGTG CCACTTCTCC AGGACCACGG ACTGCACCAG CCGCGAAGCC TGAGAAGGCG 
TCAGCTGGCA CCCGGGTTCC TGTGCAGGAG AAGACGAACT TGGCGGCCTA CGTACCCCTG 
CTGACCCAGG GCTGGGCGGA GATCCTGGTC CGGAGGCCCA CAGGGAACAC CAGCTGGCTG 
ATGAGCCTGG AGAACCCGCT CAGCCCTTTC TCTTCGGACA TCAACAACAT GCCCCTGCAG 
GAGCTGTCCA ACGCCCTCAT GGCGGCTGAG CGCTTCAAGG AGCACCGGGA CACAGCCCTG 
TACAAGTCAT TGTCGGTGCC GGCGGCCAGC ACGGCCAGAC CCCCTCCTCT GCCTCGCTCC 
AACACAGACT CCGCTGCGGT CGTGGAGGAG GGAAGTCCTG GCGAGGTTCC TGTGCCAGTG 
GAGCCCCCAG GCTTGGAGGA CTTTGAGGCA GCGCTAGGCA TGGACAGGCG CACGGATGCC 
TACAGCAGGT CGTCCTCAGT CTCCAGCCAG GAGGAGAAGT CGCTCCACGC GGAGGAGCTG 
GTTGCCAGGG GCATCCCCAT CGAGCGAGCC GTCTCCTCGG AGGGTGGCCG GCCCTCTGTG 
GACCTCTCCT TCCAGCCCTC GCAGCCCCTG AGCAAGTCCA GCTCCTCTCC CGAGCTGCAG 
ACCCTGCAGG ACATCCTCGG GGACCCTGGG GACAAGGCCG ACGTGGGCCG GCTGAGCCCT 
GAGGTTAAGG CCCGGTCACA GTCAGGGACC CTGGACGGGG AAAGTGCTGC CTGGTCAGCC 
TCGGGCGAAG ACAGTCGGGG CCAGCCGGAG GGTCCCTTGC CTTCCAGCTC CCCCCGCTCG 
CCCAGTGGCC TCCGGCCCCG AGGTTACACC ATCTCCGACT CGGCCCCATC ACGGAGGGGC 
AAGAGGGTAG AGAGGGATGC CTTTAAGAGC AGAGCCACAG CCTCCAATGC AGAGAAAGTG 
CCGGGCATCA ACCCCAGTTT CGTGTTCCTG CAGCTCTACC ATTCCCCCTT CTTTGGCGAC 
GAGTCAAACA AGCCAATCCT GCTGCCCAAT GAGTCACAGT CCTTTGAGCG GTCGGTGCAG 
CTCCTCGACC AGATCCCATC ATACGACACC CACAAGATCG CCGTCCTGTA TGTTGGAGAA 
GGCCAGAGCA ACAGCGAGCT CGCCATCCTG TCCAACGAGC ATGGCTCCTA CAGGTACACG 
GAGTTCCTGA CGGGCCTGGG CCGGCTCATC GAGCTGAAAG ACTGCCAGCC CGACAAGGTG 
TACCTGGGAG GCCTGGACGT GTGTGGTGAG GATGGCCAGT TCACCTACTG CTGGCACGAT 
GACATCATGC AAGCCGTCTT CCACATCGCG ACCCTGATGC CCACCAAGGA CGTGGACAGG 
CACCGCTGCG ACAAGAAGCG CCACCTGGGC AATGACTTTG TGTCCATTGT CTACAATGAC 
TCCGGCGAGG ACTTTAAGCT CGGCACCATC AAGGGCCAGT TCAACTTTGT CCACGTGATC 
GTCACCCCGC TGGACTATGA GTGCAACCTG GTGTCCCTGC AGTGCAGGAA AGACATGGAG 
GGCCTTGTGG ACACCAGCGT GGCCAAGATC GTGTCTGACC GCAACCTGCC CTTCGTGGCC 
CGCCAGATGG CCTTGCACGC AAATATGGCC TCACAGGTGC ACCATAGCCG CTCCAACCCC 
ACCGACATCT ACCCCTCCAA GTGGATTGCC CGGCTCCGCC ACATCAAGAG GCTCCGCCAG 
CGGATCTGTG AGGAAGCCGC CTACTCCAAC CCCAGCCTGC CTCTGGTGCA CCCCCCGTCC 
CATACCAAAG CCCCTGCACA GACTCCAGCC GAGCCCACAC CTGGCTACGA GATGGGCCAG 
CGGAAGCGCC TCGTCTCCTC CGTGGAGGAC TTCACCGAGT TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024089857.1
CDS	254..5461
Translation	MECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMVCPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTVVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVAARSLSPPPELEERDAAAHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPSGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDMPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTARPPPLPRSNTDSAAVVEEGSPGEVPVPVEPPGLEDFEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVARGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDRHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKAPAQTPAEPTPGYEMGQRKRLVSSVEDFTEFV

Target ORF information:

RefSeq Version	XM_024234089.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii TSC complex subunit 2 (TSC2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024234089.1

ORF Insert Sequence:

ATGGAATGTG GCCTCAACAA TCGCATCCGG ATGATAGGGC AGATTTGTGA AGTTGCAAAA 
ACCAAGAAAT TTGAAGAGCA CGCAGTGGAA GCACTTTGGA AGGCGGTCGC GGATCTGTTG 
CAGCCGGAGC GGCCACTGGA GGCCCGGCAC GCGGTGCTGG CTCTGCTGAA GGCCATCGTG 
CAGGGACAGG GCGAGCGTTT GGGGGTCCTC AGAGCCCTCT TCTTTAAGGT CATCAAGGAT 
TACCCTTCCA ATGAAGACCT TCACGAAAGG CTGGAAGTTT TCAAGGCCCT CACAGACAAT 
GGGAGACACA TCACCTACTT GGAGGAAGAG CTGGCTGACT TTGTCCTGCA GTGGATGGAT 
GTTGGCTTGT CCTCGGAATT CCTTCTGGTG CTGGTGAACT TGGTCAAATT CAATAGCTGT 
TACCTCGACG AGTACATCGC AAGGATGGTT CAGATGATCT GTCTGCTCTG CGTCCGGACC 
GCATCCTCTG TGGACATAGA GGTCTCCCTG CAGGTGCTGG ACGCTGTGGT CTGCTACAAC 
TGCCTGCCGG CTGAGAGCCT CCCGCTGTTC ATCGTTACTC TCTGTCGCAC CATCAACGTC 
AAGGAGCTCT GCGAGCCTTG CTGGAAGCTG ATGCGGAACC TCCTTGGCAC CCACCTGGGC 
CACAGCGCCA TCTATAACAT GTGCCACCTC ATGGAGGACA GAGCCTACAT GGAGGACGCG 
CCCCTGCTGA GAGGAGCCGT GTTTTTTGTG GGCATGGCTC TCTGGGGAGC CCACCGGCTC 
TATTCTCTGA GGAACTCGCC GACATCTGTG CTACCATCAT TTTACCAGGC CATGGTATGT 
CCGAACGAGG TGGTGTCCTA TGAGATCGTC CTGTCCATCA CCAGGCTCAT CAAGAAGTAC 
AGGAAGGAGC TCCAGGTGGT GACGTGGGAC ATTCTGCTGA ACATCATCGA ACGGCTCCTT 
CAGCAGCTCC AGACCTTGGA CAGCCCGGAG CTCAGGACCG TTGTCCATGA CCTGTTGACC 
ACGGTGGAGG AGCTGTGTGA CCAGAACGAG TTCCACGGGT CTCAGGAGAG ATACTTTGAA 
CTGGTGGAGA GATGTGCGGA CCAGAGGCCG GAGTCCTCCC TCCTGAACCT GATCTCCTAT 
AGAGCGCAGT CCATCCACCC GGCCAAGGAC GGCTGGATTC AGAACCTGCA GGCGCTGATG 
GAGAGATTCT TCAGGAGCGA GTCCCGAGGC GCCGTGCGCA TCAAGGTGCT GGACGTGCTG 
TCCTTTGTGC TGCTCATCAA CAGGCAGTTC TATGAGGAGG AGCTGATTAA CTCAGTGGTC 
ATCTCGCAGC TCTCCCACAT CCCCGAGGAT AAAGACCACC AGGTCCGAAA GCTGGCCACC 
CAGTTGCTGG TGGACCTGGC AGAGGGCTGC CACACACACC ACTTCAACAG CCTGCTGGAC 
ATCATCGAGA AGGTGGCGGC CCGCTCCCTC TCCCCACCCC CAGAGCTGGA AGAAAGGGAT 
GCGGCCGCAC ACTCAGCCTC CTTGGAGGAT GTGAAGACAG CTGTCCTGGG TCTTCTGGTC 
ATCCTTCAGA CCAAGCTGTA CACCCTGCCT GCAAGCCACG CCACGCGCGT GTATGAGATG 
CTGGTCAGCC ACATTCAGCT CCACTACAAG CACAGCTACA CCCTGCCAAT TGCGAGCAGC 
ATCCGGCTGC AGGCCTTTGA CTTCCTGTTG CTGCTGCGGG CCGACTCACT GCACCGCCTC 
GGCCTGCCCA ACAAGGATGG AGTCGTGCGG TTCAGCCCCT ACTGCGTCTG CGACTACATG 
GAGCCAGAGA GAGGCTCTGA GAAGAAGACC AGCGGCCCCC TTTCTCCTCC CTCAGGGCCG 
CCTGGCCCGG CGCCTGCAGG TCCCGCCGTG CGGCTGGGTT CCCTGCCCTA CTCCCTGCTC 
TTCCGTGTCC TGCTGCAGTG CTTGAAGCAG GAGTCTGACT GGAAGGTGCT GAAGCTGGTT 
CTGGGCAGGC TGCCCGAGTC CCTGCGCTAC AAAGTGCTCA TCTTTACTTC CCCTTGCAGT 
GTGGACCAGC TGTGCTCTGC TCTCTGCTCC ATGCTTTCAG GCCCAAAGAC ACTGGAGCGG 
CTCCGAGGCG CCCCAGAAGG CTTCTCCAGA ACTGACCTGC ACCTGGCTGT GGTTCCAGTG 
CTGACAGCAT TAATCTCTTA CCATAACTAC CTGGACAAAA CCAAACAGCG CGAGATGGTC 
TACTGCCTGG AGCAGGGCCT CATCCACCGC TGTGCCAGCC AGTGCGTCGT GGCCTTGTCC 
ATCTGCAGCG TGGAGATGCC TGACATCATC ATCAAGGCGC TGCCCGTCCT GGTGGTGAAG 
CTCACACACA TCTCGGCCAC AGCCAGCATG GCCGTCCCAC TGCTGGAGTT CCTGTCCACT 
CTGGCCAGGC TGCCGCACCT CTACAGGAAC TTTGCCGCGG AGCAGTATGC CAGTGTGTTC 
GCCATCTCCC TGCCGTACAC CAACCCCTCC AAGTTTAATC AGTACATCGT GTGTCTGGCC 
CATCACGTCA TAGCCATGTG GTTCATCAGG TGCCGCCTGC CCTTCCGGAA GGATTTCGTC 
CCTTTCATCA CTAAGGGCCT GCGGTCCAAT GTCCTCTTGT CTTTTGATGA CACCCCCGAG 
AAGGACAGCT TCAGGGCCCG GAGTACTAGT CTCAACGAGA GACCCAAGAG TCTGAGGATA 
GCCAGACCCC CCAAACAAGG CTTGAATAAC TCTCCACCCG TGAAAGAATT CAAGGAGAGC 
TCTGCAGCCG AGGCCTTCCG GTGCCGCAGC ATCAGTGTGT CTGAACATGT GGTCCGCAGC 
AGGATACAGA CGTCCCTCAC CAGTGCCAGC TTGGGGTCTG CAGACGAGAA CTCCGTGGCC 
CAGGCTGACG ATAGCCTGAA AAACCTCCAC CTGGAGCTCA CGGAAACCTG TCTGGACATG 
ATGGCTCGAT ACGTCTTCTC CAACTTCACG GCTGTCCCGA AGAGGTCTCC TGTGGGCGAG 
TTCCTCCTGG CGGGTGGCAG GACCAAAACC TGGCTGGTTG GGAACAAGCT TGTCACTGTG 
ACGACAAGCG TGGGAACCGG GACCCGGTCG TTACTAGGCC TGGACTCGGG GGAGTTGCAG 
TCCGGCCCAG AGTCGAGCTC CAGCCCCGGC GTGCATGTGA GACAGACAAA GGAGGCGCCG 
GCCAAGCTGG AGTCCCAGGC TGGGCAGCAG GTGTCCCGTG GGGCCCGGGA CCGGGTCCGT 
TCCATGTCGG GGGGCCATGG TCTTCGAGTT GGCGCCCTGG ACATGCCGGC CTCCCAGTTC 
CTGGGCAGTG CCACTTCTCC AGGACCACGG ACTGCACCAG CCGCGAAGCC TGAGAAGGCG 
TCAGCTGGCA CCCGGGTTCC TGTGCAGGAG AAGACGAACT TGGCGGCCTA CGTACCCCTG 
CTGACCCAGG GCTGGGCGGA GATCCTGGTC CGGAGGCCCA CAGGGAACAC CAGCTGGCTG 
ATGAGCCTGG AGAACCCGCT CAGCCCTTTC TCTTCGGACA TCAACAACAT GCCCCTGCAG 
GAGCTGTCCA ACGCCCTCAT GGCGGCTGAG CGCTTCAAGG AGCACCGGGA CACAGCCCTG 
TACAAGTCAT TGTCGGTGCC GGCGGCCAGC ACGGCCAGAC CCCCTCCTCT GCCTCGCTCC 
AACACAGACT CCGCTGCGGT CGTGGAGGAG GGAAGTCCTG GCGAGGTTCC TGTGCCAGTG 
GAGCCCCCAG GCTTGGAGGA CTTTGAGGCA GCGCTAGGCA TGGACAGGCG CACGGATGCC 
TACAGCAGGT CGTCCTCAGT CTCCAGCCAG GAGGAGAAGT CGCTCCACGC GGAGGAGCTG 
GTTGCCAGGG GCATCCCCAT CGAGCGAGCC GTCTCCTCGG AGGGTGGCCG GCCCTCTGTG 
GACCTCTCCT TCCAGCCCTC GCAGCCCCTG AGCAAGTCCA GCTCCTCTCC CGAGCTGCAG 
ACCCTGCAGG ACATCCTCGG GGACCCTGGG GACAAGGCCG ACGTGGGCCG GCTGAGCCCT 
GAGGTTAAGG CCCGGTCACA GTCAGGGACC CTGGACGGGG AAAGTGCTGC CTGGTCAGCC 
TCGGGCGAAG ACAGTCGGGG CCAGCCGGAG GGTCCCTTGC CTTCCAGCTC CCCCCGCTCG 
CCCAGTGGCC TCCGGCCCCG AGGTTACACC ATCTCCGACT CGGCCCCATC ACGGAGGGGC 
AAGAGGGTAG AGAGGGATGC CTTTAAGAGC AGAGCCACAG CCTCCAATGC AGAGAAAGTG 
CCGGGCATCA ACCCCAGTTT CGTGTTCCTG CAGCTCTACC ATTCCCCCTT CTTTGGCGAC 
GAGTCAAACA AGCCAATCCT GCTGCCCAAT GAGTCACAGT CCTTTGAGCG GTCGGTGCAG 
CTCCTCGACC AGATCCCATC ATACGACACC CACAAGATCG CCGTCCTGTA TGTTGGAGAA 
GGCCAGAGCA ACAGCGAGCT CGCCATCCTG TCCAACGAGC ATGGCTCCTA CAGGTACACG 
GAGTTCCTGA CGGGCCTGGG CCGGCTCATC GAGCTGAAAG ACTGCCAGCC CGACAAGGTG 
TACCTGGGAG GCCTGGACGT GTGTGGTGAG GATGGCCAGT TCACCTACTG CTGGCACGAT 
GACATCATGC AAGCCGTCTT CCACATCGCG ACCCTGATGC CCACCAAGGA CGTGGACAGG 
CACCGCTGCG ACAAGAAGCG CCACCTGGGC AATGACTTTG TGTCCATTGT CTACAATGAC 
TCCGGCGAGG ACTTTAAGCT CGGCACCATC AAGGGCCAGT TCAACTTTGT CCACGTGATC 
GTCACCCCGC TGGACTATGA GTGCAACCTG GTGTCCCTGC AGTGCAGGAA AGACATGGAG 
GGCCTTGTGG ACACCAGCGT GGCCAAGATC GTGTCTGACC GCAACCTGCC CTTCGTGGCC 
CGCCAGATGG CCTTGCACGC AAATATGGCC TCACAGGTGC ACCATAGCCG CTCCAACCCC 
ACCGACATCT ACCCCTCCAA GTGGATTGCC CGGCTCCGCC ACATCAAGAG GCTCCGCCAG 
CGGATCTGTG AGGAAGCCGC CTACTCCAAC CCCAGCCTGC CTCTGGTGCA CCCCCCGTCC 
CATACCAAAG CCCCTGCACA GACTCCAGCC GAGCCCACAC CTGGCTACGA GATGGGCCAG 
CGGAAGCGCC TCGTCTCCTC CGTGGAGGAC TTCACCGAGT TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSC2 ( XM_009250325.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009248600.1 Pongo abelii tuberous sclerosis 2 (TSC2), mRNA.

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CloneID	OSa28464
Clone ID Related Accession (Same CDS sequence)	XM_009250325.1
Accession Version	XM_009250325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	LOW QUALITY PROTEIN: tuberin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002888003.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT TGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTTTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CACTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGCG AGCGTTTGGG GATCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAATG AAGACCTTCA CGAAAGGCTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTCTGCGT CCGGACCGCA TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACTCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ATAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGATGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTGAGGA ACTCGCCGAC ATCTGTGCTA 
CCATCATTTT ACCAGGCCAT GGTATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTACAGG AAGGAGCTCC AGGTGGTGAC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCGTTG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCGGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCT TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGGCGGCCCG CTCCCTCTCC 
CCACCCCCAG AGCTGGAAGA AAGGGATGCG GCCGCACACT CAGCCTCCTT GGAGGATGTG 
AAGACAGCTG TCCTGGGTCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGCGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATTGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTCGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCTC AGGGCCGCCT GGCCCGGCGC CTGCAGGTCC CGCCGTGCGG 
CTGGGTTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CCGAGTCCCT GCGCTACAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACCTGCACC TGGCTGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CCGTCCTGGT GGTGAAGCTC ACACACATCT CGGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG 
GGGTCTGCAG ACGAGAACTC CGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG 
GAGCTCACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT 
GTCCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTGGCGG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA 
CTAGGCCTGG ACTCGGGGGA GTTGCAGTCC GGCCCAGAGT CGAGCTCCAG CCCCGGCGTG 
CATGTGAGAC AGACAAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGTTCC ATGTCAGGGG GCCATGGTCT TCGAGTTGGC 
GCCCTGGACA TGCCGGCCTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT 
GCACCAGCCG CGAAGCCTGA GAAGGCGTCA GCTGGCACCC GGGTTCCTGT GCAGGAGAAG 
ACGAACTTGG CGGCCTACGT ACCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCT 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAACG CCCTCATGGC GGCTGAGCGC 
TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCATTGT CGGTGCCGGC GGCCAGCACG 
GCCAGACCCC CTCCTCTGCC TCGCTCCAAC ACAGACTCCG CTGCGGTCGT GGAGGAGGGA 
AGTCCTGGCG AGGTTCCTGT GCCAGTGGAG CCCCCAGGCT TGGAGGACTT TGAGGCAGCG 
CTAGGCATGG ACAGGCGCAC GGATGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG 
GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GCCAGGGGCA TCCCCATCGA GCGAGCCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC 
AAGTCCAGCT CCTCTCCCGA GCTGCAGACC CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG 
GACGGGGAAA GTGCTGCCTG GTCAGCCTCG GGCGAAGACA GTCGGGGCCA GCCGGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC 
TCCGACTCGG CCCCATCACG GAGGGGCAAG AGGGTAGAGA GGGATGCCTT TAAGAGCAGA 
GCCACAGCCT CCAATGCAGA GAAAGTGCCG GGCATCAACC CCAGGTGGGC CTCTTGCTTC 
CGGGTGGGGC TCCTGACACC TGCGTCTGGG GCTCAGGCAG GACTCTGTGT GCTACAGTCA 
CAGTCCTTTG AGCGGTCGGT GCAGCTCCTC GACCAGATCC CATCATACGA CACCCACAAG 
ATCGCCGTCC TGTATGTTGG AGAAGGCCAG AGCAACAGCG AGCTCGCCAT CCTGTCCAAC 
GAGCATGGCT CCTACAGGTA CACGGAGTTC CTGACGGGCC TGGGCCGGCT CATCGAGCTG 
AAAGACTGCC AGCCCGACAA GGTGTACCTG GGAGGCCTGG ACGTGTGTGG TGAGGATGGT 
CAGTTCACCT ACTGCTGGCA CGATGACATC ATGCATGGTA CGGCCTGGCT CCCACACATG 
CTGCCCGTGG AGCCGACAGG CGTCTAGCAG TGCAACCAGG CGGTGGCCGA GAGCAGCCTT 
CAGCACATGC TGTGTGTGGG GATGACCCCC CTTCTCTTGT CCGGGCAGGG CCAGTTCAAC 
TTTGTCCACG TGATCGTCAC CCCGCTGGAC TATGAGTGCA ACCTGGTGTC CCTGCAGTGC 
AGGAAAGACA TGGAGGGCCT TGTGGACACC AGCGTGGCCA AGATCGTGTC TGACCGCAAC 
CTGCCCTTCG TGGCCCGCCA GATGGCCCTG CACGCAAATA TGGCCTCACA GGTGCACCAT 
AGCCGCTCCA ACCCCACCGA CATCTACCCC TCCAAGTGGA TTGCCCGGCT CCGCCACATC 
AAGAGGCTCC GCCAGCGGAT CTGTGAGGAA GCCGCCTACT CCAACCCCAG CCTGCCTCTG 
GTGCACCCCC CGTCCCATAC CAAAGCCCCT GCACAGACTC CAGCCGAGCC CACACCTGGC 
TACGAGATGG GCCAGCGGAA GCGCCTCGTC TCCTCCGTGG AGGACTTCAC CGAGTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009248600.1
CDS	194..5536
Translation	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGILRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMVCPNEVVSYEIVLSITRLIKKYRKELQVVTWDILLNIIERLLQQLQTLDSPELRTVVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVAARSLSPPPELEERDAAAHSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPSGPPGPAPAGPAVRLGSLPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDMPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTARPPPLPRSNTDSAAVVEEGSPGEVPVPVEPPGLEDFEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVARGIPIERAVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDAFKSRATASNAEKVPGINPRWASCFRVGLLTPASGAQAGLCVLQSQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMHGTAWLPHMLPVEPTGV*QCNQAVAESSLQHMLCVGMTPLLLSGQGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHTKAPAQTPAEPTPGYEMGQRKRLVSSVEDFTEFV

Target ORF information:

RefSeq Version	XM_009250325.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii tuberous sclerosis 2 (TSC2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009250325.1

ORF Insert Sequence:

ATGGCCAAAC CAACAAGCAA AGATTCAGGC TTGAAGGAGA AGTTTAAGAT TCTGTTGGGA 
CTGGGAACAC CGAGGCCAAA TCCCAGGTCT GCAGAGGGTA AACAGACGGA GTTTATCATC 
ACCGCGGAAA TACTGAGAGA ACTGAGCATG GAATGTGGCC TCAACAATCG CATCCGGATG 
ATAGGGCAGA TTTGTGAAGT TGCAAAAACC AAGAAATTTG AAGAGCACGC AGTGGAAGCA 
CTTTGGAAGG CGGTCGCGGA TCTGTTGCAG CCGGAGCGGC CACTGGAGGC CCGGCACGCG 
GTGCTGGCTC TGCTGAAGGC CATCGTGCAG GGACAGGGCG AGCGTTTGGG GATCCTCAGA 
GCCCTCTTCT TTAAGGTCAT CAAGGATTAC CCTTCCAATG AAGACCTTCA CGAAAGGCTG 
GAAGTTTTCA AGGCCCTCAC AGACAATGGG AGACACATCA CCTACTTGGA GGAAGAGCTG 
GCTGACTTTG TCCTGCAGTG GATGGATGTT GGCTTGTCCT CGGAATTCCT TCTGGTGCTG 
GTGAACTTGG TCAAATTCAA TAGCTGTTAC CTCGACGAGT ACATCGCAAG GATGGTTCAG 
ATGATCTGTC TGCTCTGCGT CCGGACCGCA TCCTCTGTGG ACATAGAGGT CTCCCTGCAG 
GTGCTGGACG CTGTGGTCTG CTACAACTGC CTGCCGGCTG AGAGCCTCCC GCTGTTCATC 
GTTACTCTCT GTCGCACCAT CAACGTCAAG GAGCTCTGCG AGCCTTGCTG GAAGCTGATG 
CGGAACCTCC TTGGCACCCA CCTGGGCCAC AGCGCCATCT ATAACATGTG CCACCTCATG 
GAGGACAGAG CCTACATGGA GGATGCGCCC CTGCTGAGAG GAGCCGTGTT TTTTGTGGGC 
ATGGCTCTCT GGGGAGCCCA CCGGCTCTAT TCTCTGAGGA ACTCGCCGAC ATCTGTGCTA 
CCATCATTTT ACCAGGCCAT GGTATGTCCG AACGAGGTGG TGTCCTATGA GATCGTCCTG 
TCCATCACCA GGCTCATCAA GAAGTACAGG AAGGAGCTCC AGGTGGTGAC GTGGGACATT 
CTGCTGAACA TCATCGAACG GCTCCTTCAG CAGCTCCAGA CCTTGGACAG CCCGGAGCTC 
AGGACCGTTG TCCATGACCT GTTGACCACG GTGGAGGAGC TGTGTGACCA GAACGAGTTC 
CACGGGTCTC AGGAGAGATA CTTTGAACTG GTGGAGAGAT GTGCGGACCA GAGGCCGGAG 
TCCTCCCTCC TGAACCTGAT CTCCTATAGA GCGCAGTCCA TCCACCCGGC CAAGGACGGC 
TGGATTCAGA ACCTGCAGGC GCTGATGGAG AGATTCTTCA GGAGCGAGTC CCGAGGCGCC 
GTGCGCATCA AGGTGCTGGA CGTGCTGTCT TTTGTGCTGC TCATCAACAG GCAGTTCTAT 
GAGGAGGAGC TGATTAACTC AGTGGTCATC TCGCAGCTCT CCCACATCCC CGAGGATAAA 
GACCACCAGG TCCGAAAGCT GGCCACCCAG TTGCTGGTGG ACCTGGCAGA GGGCTGCCAC 
ACACACCACT TCAACAGCCT GCTGGACATC ATCGAGAAGG TGGCGGCCCG CTCCCTCTCC 
CCACCCCCAG AGCTGGAAGA AAGGGATGCG GCCGCACACT CAGCCTCCTT GGAGGATGTG 
AAGACAGCTG TCCTGGGTCT TCTGGTCATC CTTCAGACCA AGCTGTACAC CCTGCCTGCA 
AGCCACGCCA CGCGCGTGTA TGAGATGCTG GTCAGCCACA TTCAGCTCCA CTACAAGCAC 
AGCTACACCC TGCCAATTGC GAGCAGCATC CGGCTGCAGG CCTTTGACTT CCTGTTGCTG 
CTGCGGGCCG ACTCACTGCA CCGCCTCGGC CTGCCCAACA AGGATGGAGT CGTGCGGTTC 
AGCCCCTACT GCGTCTGCGA CTACATGGAG CCAGAGAGAG GCTCTGAGAA GAAGACCAGC 
GGCCCCCTTT CTCCTCCCTC AGGGCCGCCT GGCCCGGCGC CTGCAGGTCC CGCCGTGCGG 
CTGGGTTCCC TGCCCTACTC CCTGCTCTTC CGTGTCCTGC TGCAGTGCTT GAAGCAGGAG 
TCTGACTGGA AGGTGCTGAA GCTGGTTCTG GGCAGGCTGC CCGAGTCCCT GCGCTACAAA 
GTGCTCATCT TTACTTCCCC TTGCAGTGTG GACCAGCTGT GCTCTGCTCT CTGCTCCATG 
CTTTCAGGCC CAAAGACACT GGAGCGGCTC CGAGGCGCCC CAGAAGGCTT CTCCAGAACT 
GACCTGCACC TGGCTGTGGT TCCAGTGCTG ACAGCATTAA TCTCTTACCA TAACTACCTG 
GACAAAACCA AACAGCGCGA GATGGTCTAC TGCCTGGAGC AGGGCCTCAT CCACCGCTGT 
GCCAGCCAGT GCGTCGTGGC CTTGTCCATC TGCAGCGTGG AGATGCCTGA CATCATCATC 
AAGGCGCTGC CCGTCCTGGT GGTGAAGCTC ACACACATCT CGGCCACAGC CAGCATGGCC 
GTCCCACTGC TGGAGTTCCT GTCCACTCTG GCCAGGCTGC CGCACCTCTA CAGGAACTTT 
GCCGCGGAGC AGTATGCCAG TGTGTTCGCC ATCTCCCTGC CGTACACCAA CCCCTCCAAG 
TTTAATCAGT ACATCGTGTG TCTGGCCCAT CACGTCATAG CCATGTGGTT CATCAGGTGC 
CGCCTGCCCT TCCGGAAGGA TTTCGTCCCT TTCATCACTA AGGGCCTGCG GTCCAATGTC 
CTCTTGTCTT TTGATGACAC CCCCGAGAAG GACAGCTTCA GGGCCCGGAG TACTAGTCTC 
AACGAGAGAC CCAAGAGTCT GAGGATAGCC AGACCCCCCA AACAAGGCTT GAATAACTCT 
CCACCCGTGA AAGAATTCAA GGAGAGCTCT GCAGCCGAGG CCTTCCGGTG CCGCAGCATC 
AGTGTGTCTG AACATGTGGT CCGCAGCAGG ATACAGACGT CCCTCACCAG TGCCAGCTTG 
GGGTCTGCAG ACGAGAACTC CGTGGCCCAG GCTGACGATA GCCTGAAAAA CCTCCACCTG 
GAGCTCACGG AAACCTGTCT GGACATGATG GCTCGATACG TCTTCTCCAA CTTCACGGCT 
GTCCCGAAGA GGTCTCCTGT GGGCGAGTTC CTCCTGGCGG GTGGCAGGAC CAAAACCTGG 
CTGGTTGGGA ACAAGCTTGT CACTGTGACG ACAAGCGTGG GAACCGGGAC CCGGTCGTTA 
CTAGGCCTGG ACTCGGGGGA GTTGCAGTCC GGCCCAGAGT CGAGCTCCAG CCCCGGCGTG 
CATGTGAGAC AGACAAAGGA GGCGCCGGCC AAGCTGGAGT CCCAGGCTGG GCAGCAGGTG 
TCCCGTGGGG CCCGGGACCG GGTCCGTTCC ATGTCAGGGG GCCATGGTCT TCGAGTTGGC 
GCCCTGGACA TGCCGGCCTC CCAGTTCCTG GGCAGTGCCA CTTCTCCAGG ACCACGGACT 
GCACCAGCCG CGAAGCCTGA GAAGGCGTCA GCTGGCACCC GGGTTCCTGT GCAGGAGAAG 
ACGAACTTGG CGGCCTACGT ACCCCTGCTG ACCCAGGGCT GGGCGGAGAT CCTGGTCCGG 
AGGCCCACAG GGAACACCAG CTGGCTGATG AGCCTGGAGA ACCCGCTCAG CCCTTTCTCT 
TCGGACATCA ACAACATGCC CCTGCAGGAG CTGTCCAACG CCCTCATGGC GGCTGAGCGC 
TTCAAGGAGC ACCGGGACAC AGCCCTGTAC AAGTCATTGT CGGTGCCGGC GGCCAGCACG 
GCCAGACCCC CTCCTCTGCC TCGCTCCAAC ACAGACTCCG CTGCGGTCGT GGAGGAGGGA 
AGTCCTGGCG AGGTTCCTGT GCCAGTGGAG CCCCCAGGCT TGGAGGACTT TGAGGCAGCG 
CTAGGCATGG ACAGGCGCAC GGATGCCTAC AGCAGGTCGT CCTCAGTCTC CAGCCAGGAG 
GAGAAGTCGC TCCACGCGGA GGAGCTGGTT GCCAGGGGCA TCCCCATCGA GCGAGCCGTC 
TCCTCGGAGG GTGGCCGGCC CTCTGTGGAC CTCTCCTTCC AGCCCTCGCA GCCCCTGAGC 
AAGTCCAGCT CCTCTCCCGA GCTGCAGACC CTGCAGGACA TCCTCGGGGA CCCTGGGGAC 
AAGGCCGACG TGGGCCGGCT GAGCCCTGAG GTTAAGGCCC GGTCACAGTC AGGGACCCTG 
GACGGGGAAA GTGCTGCCTG GTCAGCCTCG GGCGAAGACA GTCGGGGCCA GCCGGAGGGT 
CCCTTGCCTT CCAGCTCCCC CCGCTCGCCC AGTGGCCTCC GGCCCCGAGG TTACACCATC 
TCCGACTCGG CCCCATCACG GAGGGGCAAG AGGGTAGAGA GGGATGCCTT TAAGAGCAGA 
GCCACAGCCT CCAATGCAGA GAAAGTGCCG GGCATCAACC CCAGGTGGGC CTCTTGCTTC 
CGGGTGGGGC TCCTGACACC TGCGTCTGGG GCTCAGGCAG GACTCTGTGT GCTACAGTCA 
CAGTCCTTTG AGCGGTCGGT GCAGCTCCTC GACCAGATCC CATCATACGA CACCCACAAG 
ATCGCCGTCC TGTATGTTGG AGAAGGCCAG AGCAACAGCG AGCTCGCCAT CCTGTCCAAC 
GAGCATGGCT CCTACAGGTA CACGGAGTTC CTGACGGGCC TGGGCCGGCT CATCGAGCTG 
AAAGACTGCC AGCCCGACAA GGTGTACCTG GGAGGCCTGG ACGTGTGTGG TGAGGATGGT 
CAGTTCACCT ACTGCTGGCA CGATGACATC ATGCATGGTA CGGCCTGGCT CCCACACATG 
CTGCCCGTGG AGCCGACAGG CGTCTAGCAG TGCAACCAGG CGGTGGCCGA GAGCAGCCTT 
CAGCACATGC TGTGTGTGGG GATGACCCCC CTTCTCTTGT CCGGGCAGGG CCAGTTCAAC 
TTTGTCCACG TGATCGTCAC CCCGCTGGAC TATGAGTGCA ACCTGGTGTC CCTGCAGTGC 
AGGAAAGACA TGGAGGGCCT TGTGGACACC AGCGTGGCCA AGATCGTGTC TGACCGCAAC 
CTGCCCTTCG TGGCCCGCCA GATGGCCCTG CACGCAAATA TGGCCTCACA GGTGCACCAT 
AGCCGCTCCA ACCCCACCGA CATCTACCCC TCCAAGTGGA TTGCCCGGCT CCGCCACATC 
AAGAGGCTCC GCCAGCGGAT CTGTGAGGAA GCCGCCTACT CCAACCCCAG CCTGCCTCTG 
GTGCACCCCC CGTCCCATAC CAAAGCCCCT GCACAGACTC CAGCCGAGCC CACACCTGGC 
TACGAGATGG GCCAGCGGAA GCGCCTCGTC TCCTCCGTGG AGGACTTCAC CGAGTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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1-732-210-0262

TSC2 cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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