NUP214 cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene
Clones
gRNAs

Summary

Gene Symbol	NUP214
Entrez Gene ID	100442200
Full Name	nucleoporin 214
Gene Type	protein-coding
Organism	Pongo abelii(Sumatran orangutan)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024252127.1	XP_024107895.1	nuclear pore complex protein Nup214 isoform X6
XM_024252123.1	XP_024107891.1	nuclear pore complex protein Nup214 isoform X2
XM_024252126.1	XP_024107894.1	nuclear pore complex protein Nup214 isoform X5
XM_024252122.1	XP_024107890.1	nuclear pore complex protein Nup214 isoform X1
XM_024252125.1	XP_024107893.1	nuclear pore complex protein Nup214 isoform X4
XM_002820311.3	XP_002820357.2	nuclear pore complex protein Nup214
XM_024252124.1	XP_024107892.1	nuclear pore complex protein Nup214 isoform X3

Pathways from BioSystems
Gene Ontology

The following NUP214 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NUP214 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa186576	XM_024252122.1 Latest version!	Pongo abelii nucleoporin 214 (NUP214), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa186577	XM_024252123.1 Latest version!	Pongo abelii nucleoporin 214 (NUP214), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa186578	XM_024252124.1 Latest version!	Pongo abelii nucleoporin 214 (NUP214), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa186579	XM_024252125.1 Latest version!	Pongo abelii nucleoporin 214 (NUP214), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa186580	XM_024252126.1 Latest version!	Pongo abelii nucleoporin 214 (NUP214), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa186581	XM_024252127.1 Latest version!	Pongo abelii nucleoporin 214 (NUP214), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa08300	XM_002820311.3 Latest version!	Pongo abelii nucleoporin 214kDa (NUP214), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NUP214 ( XM_024252122.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024107890.1 Pongo abelii nucleoporin 214 (NUP214), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa186576
Clone ID Related Accession (Same CDS sequence)	XM_024252122.1
Accession Version	XM_024252122.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	nuclear pore complex protein Nup214 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036912.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTCGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTTGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAATAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGTGTGC ATGATGTCCG GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGGTTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCAATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTC ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGATTAA TTGGGAATCT 
TGGCTACTGG AGGATTCTAG TCGAGCTGAA TTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GAGTTGCCGT AGACTATACG AACCAAGTGG AAATCACCAT CAGTGATGAA 
AAGACTCTTC CTCCTGCTCC AGTTCTCATG TTACTTTCAA CAGATGGTGT GCTTTGTCCA 
TTTTATATGA TTAATCAAAA TCCTGGGGTT AAGTCTCTCA TCAAAACACC AGAGCGACTT 
TCATTAGAAG GAGAGCGACA GCCCAAGTCA CCAGGAAGTA CTCCCACTAC CCCGACCTCC 
TCTCAAGCCC CGCAGAAACT GGATGCTTCT GCAGCTGCAG CCCCTGCCTC TCTTCCACCT 
TCATCACCCG CTGCTCCCAT TGCCACTTTT TCTTTGCTTC CTGCTGGTGG AGCCCCCACT 
GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG TCATCTGCTG CGGTCACTGG GGAGCCCCCT 
TCATATTCCA GTGGCTCCGA CAGCTCCAAA GCAGCCCCGG GCCCTGGCCC ATCAACCTTC 
TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA GCCCCCACCC CTGCAGCGTC TCCCGTGGCT 
CCATCAGCTG CTTCATTCTC GTTTGGATCA TCTGGTTTTA AGCCTACCCT GGAAAGCACA 
CCAGTGCCAA GTGTGTCTGC TCCAAATATA GCAGTGAAGC CCTCCTTCCC ACCCTCAACC 
TCTGCTGTCA AAGTCAACCT TAGTGAAAAG TTTACTGCCG CAGCTACCTC TACTCCTGTT 
AGTAGCTCCC AGAGCGCACC CTCGATGCCG CCATTCTCTT CTGCCTCCAA GCCAGCTACT 
TCTGGACCGC TCAGCCACCC CACGCCTCTC TCAGCATCAC CTAGTTCCGT GCCATTGAAG 
TCCTCAGTCT TGCCCTCACC ATCAGCAGGA CGATCTGCTC AGGGCAGTCC AAGCCCAGTG 
CCCTCAATGG TACAGAAATC ACCCAGGATA ACCCCTCCAG TGGCAAAGCC AGGCTCTCCC 
CAGGCAAAGT CACTTCAGCC TGCTGTTGCA GAGAAGCAGG GACATCAGTG GAAAGATTCA 
GATCCTGTAA TGGCTGGAAT TGGGGAGGAG ATTGCACACT TTCAGAAGGA GTTGGAAGAG 
TTAAAAGCCC GAACTTCCAA AGCCTGTTTC CAAGTGGGCA CTTCTGAGGA GATGAAGATG 
CTGCGAACAG AATCAGATGA CTTGCATACC TTTCTTTTGG AGATTAAAGA GACCACAGAG 
TCGCTTCATG GAGACATAAG TAGCCTGAAA ACAACTTTAC TTGAGGGCTT TGCTGGTGTT 
GAGGAAGCCA GAGAACAAAA TGAAAGAAAT CGTGACTCTG GTTATCTGCA TTTGCTTTAT 
AAAAGACCAC TGGATCCCAA GAGTGAAGCT CAGCTTCAGG AAATTCGGCG CCTTCATCAG 
TATGTGAAAT TTGCTGTCCA AGATGTGAAT GATGTTCTAG ATTTGGAGTG GGATCGGCAT 
CTGGAACAAA AGAAAAAACA AAGGCACCTG CTTGTGCCAG AGCGAGAGAC ACTGTTTAAC 
ACCCTAGCCA ACAACCGGGA AATCATCAAC CAACAGAGGA AGAGGCTGAA TCACCTGGTG 
GACAGTCTTC AGCAGCTCCG CCTTTACAAC CAGACTTCCC CGTGGAGCCT GCCCTCGGCT 
GTTCCTTCCC AGAGCAGCAT TCACAGTTTT GACAGTGACC TTGAAAGCCT GCGCAATGCT 
TTGTTGAAAA CCACCATAGA ATCTCACACC AAATCCTTGC CCAAAGTACC AGCCAAACTG 
TCCCCCATGA AACAGGCACA ACTGAGAAAC TTCTTGGCCA AGAGGAAGAC CCCACCAGTG 
AGATCCACTG CTCCAGCCAG CCTGTCTCGA TCAGCCTTTC TGTCTCAGAG ATATTATGAA 
GACTTGGATG AAGTCAGCTC AACGTCATCT GTCTCCCAGT CTCTGGAGAG TGAAGATGCA 
CGGACGTCCT ATAAAGATGA GGAGGCAGTG GTTCAGGCCC CTCGGCACGC CCCCGTGGTT 
CGCACTCCTT CCGTCCAGCC CAGTCTCTTG CCCCATGCAG CACCTTTTGC TAAATCTCAC 
CTGGTTCATG GTTCTTCACC TGGTGTGATG GGAACTTCAG TGGCTACATC TGCTAGCAAA 
ATTATTCCTC AAGGGGCCGA TAGCACAATG CTTGCCACGA AAACCGTGAA ACATGGTGCA 
CCTAGTCCTT CCCACCCCAT CTCAGCCCCG CAGGCAGCTG CCGCAGCAGC ACTCAGGCGG 
CAGATGGCCA GTCAGGCACC AGCTGTAAAC ACTTTGACTG AATCAACGTT GAAGAATGTC 
CCTCAAGTGG TAAATGTGCA GGAATTGAAG AATAACCCTG CAACCCCTTC TACAGCCATG 
GGTTCTTCAG TGGCCTACTC CGCAGCCAAA ACACCTCACC CAGTGTTGAC CCCAGTGGCC 
GCTAACCAAG CCAAGCAGGG GTCTCTAATA AATCCCCTTA AGCCATCTGG GCCTACACCA 
GCATCCGGTC AGTTATCATC TGGTGACAAA GCTTCAGGGA CAGCCAAGAT AGAAACAGCT 
GTGACTTCGA CCCCATCTGC TTCTGGGCAG TTCAGCAAGC CTTTCTCATT TTCTCCATCA 
GGGACTGGCT TTAATTTTGG GATAATCACA CCAACACCGT CTTCTAATTT CACTGCTGCA 
CAAGGGGCAA CACCCTCCAC TAAAGAGTCA AGCCAGCTGG ATGCATTCTC ATTTGGTGGG 
GGAAGCAAAC CTTCTTATGA GGCCATTCCT GAAAGCTCAC CTCCCTCAGG AATCACATCC 
GCATCAAACA CCACCCCAGG AGAACCTGCC ACATCTAGCA GCAGACCTGT GGCACCTTCT 
GGAACTGCTC TTTCCACTAC CTCTAGTAAG CTGGAAACCC CACCGTCCAA GCTGGGAGAG 
CTTCTGTTTC CAAGTTCTTT GGCTGGAGAG ACTCTGGGAA GTTTTTCAGG ACTGCGTGTT 
GGCCAAGCAG ATGATTCTAC AAAGCCAACC AATAAGGCTT CATCCACAAG CCTAACTAGT 
ACCCAGCCAA CCAAGACGTC AGGCGTGCCC TCAGGGTTTA ATTTTACTGC CCCCCCAGTG 
TTAGGGAAGC AAACGGAGCC CCCTGTGACC TCCTCTGCAA CCACCACCTC AGTAGCACCA 
CCAGCAGCCA CCAGCACTTC CTCAACTGGC GTTTTTGGCA GTCTGCCGGT CACCAGTGCA 
GGATCCTCTG GGGTCATCAG TTTTGGTGGG ACATCTCTAA GTGGTGGCAA GACTAGTTTT 
TCATTTGGAA GCCAACAGAC CAATAGCACA GTGCCCCCAT CTGCCCCACC GCCTACTACA 
GCTGCCACTC CCCTTCCAAC ATCATTCCCC ACATTGTCGT TCGGTAGCCT CCTGAGTTCA 
GCAACTACCC CCTCCCTGCC TATGTCCTCT GGCAGAAGCA CAGAAGAGGC CACTTCATCA 
GCTTTGCCTG AGAAGCCAGG TGACAGTGAG GTCTCAGCAT CAGCAGCCTC ACTTCTAGAG 
GAGCAACAGT CAGCCCAGCT TCCCCAGGCT CCTCCGCAAA CTTCTGACTC TGTTAAAAAA 
GAACCTGCTC TTGCCCAGCC TGCAGTCAGC AACTCTGGCA CTGCAGCATC TAGTACTAGT 
CTTGTAGCAC TTTCTGCAGA GGCTACCCCA ACCACCACCG GGGTCCCTGA TGCCAGGTCG 
GAGGCAGTAC CACCTGCTTC CTCCTTTTCT GTGCCTGGGC AGACTGCTGT CACAGCAGCT 
GCTATCTCAA GTGCAGGCCC TGTGGCCGTC GAAACATCAA GTGCCCCCAT AACCTCCAGC 
ACCACGTCCG TTGTTGCTCC CGGCCCATCT GCAGAGGCAG CAGCATTTGG TACCGTCACT 
TCTGGCTCAT CAGTCTTTGC TCAGCCCCCT GCTGCCAGTT CTAGCTCAGC TTTCAACCAG 
CTCACCAACA ACACAGCCAC TGCCACTTCT GCCACGCCCG TGTTTGGGCA AGTGGCAGCC 
AGCACTGCAC CAAGTCTGTT TGGGCAGCAG ACTGGTAGCA CAGCCAGCAC AGCAGCTGCC 
ACACCACAGG TCAGCAGCTC AGGGTTTAGC AGCCCAGCTT TTGGCACCAC AGCCCCAGGG 
GTCTTTGGAC AGACAACCTT CGGGCAGGCC TCAGTGTTTG GGCAGTCAGC GAGCAGTGCC 
GCAAGTGGCT TTTCCTTCAG TCAGCCTGGG TTCAGTTCCG TGCCTGCCTT CGGTCAGCCT 
GCTTCCTCCA CCCCCACATC CACCAGTGGA AGTGTCTTTG GTGCCGCCTC AAGTACCAGT 
AGCTCCAGTT CCTTCTCATT TGGACAGTCT TCTGCCAACA CAGGAGGGGG GCTGTTTGGC 
CAAAGCAACG CTCCTGCTTT TGGGCAGAGT CCTGGCTTTG GACAGGGAGG CTCTGTATTT 
GGTGGTACCT CAGCTGCCAC CACAACAGCA GTATCCTCTG GGTTCAGCTT TTGTCAAGCT 
TCAGGTTTTG GGTCTAGTAA TACTGGTTCT GTGTTTGGTC AAGCAGCCAG TACTGGTGGA 
ATAGTCTTTG GCCAGCAGTC ATCGTCTTCC AGTGGTAGCG TGTTTGGGTC TGGAAACACT 
GGAAGAGGGG GAGGTTTCTT CAGTGGCCTT GGAGGAAAAC CCAGTCAGGA TGCAGCCAAC 
AAAAACCCAT TCAGCTCGGC CAGTGGGGGC TTTGGATCCA CAGCTACCTC AAATACCTCT 
AACCTATTTG GAAACAGTGG GGCCAAGACA TTTGGTGGAT TTGCCAGCTC GTCGTTTGGA 
GAGCAGAAAC CCACTGGCAC TTTCAGCTCT GGAGGAGGAA GTGTGGCATC CCAAGGCTTT 
GGGTTTTCCT CTCCAAACAA AACAGGTGGC TTCGGTGCTG CTCCAGTGTT TGGCAGCCCT 
CCTACTTTTG GGGGATCCCC TGGGTTTGGA GGGGTGCCAG CATTCGGTTC AGCCCCAGCC 
TTTACAAGCC CTCTGGGCTC GACGGGAGGC AAAGTGTTCG GAGAGGGCAC TGCGGCTGCC 
AGCGCAGGAG GATTCGGGTT TGGGAGCAGC AGCAACACCA CATCCTTCGG CACGCTCGCG 
AGTCAGAATG CCCCCACTTT CGGATCACTG TCCCAACAGA CTTCTGGTTT TGGGACCCAG 
AGTAGCGGAT TCTCTGGTTT TGGATCAGGC ACAGGAGGGT TCAGCTTTGG ATCAAATAAC 
TCATCTGTCC AGGGTTTTGG TGGCTGGCGA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024107890.1
CDS	139..6414
Translation	MGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSVCMMSGEYGSIIAFFDVRTFSNEAKQQKRPFAYHKLLKDAGGMVVDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQINWESWLLEDSSRAELPVTDKSDDSLPMGVAVDYTNQVEITISDEKTLPPAPVLMLLSTDGVLCPFYMINQNPGVKSLIKTPERLSLEGERQPKSPGSTPTTPTSSQAPQKLDASAAAAPASLPPSSPAAPIATFSLLPAGGAPTVFSFGSSSLKSSAAVTGEPPSYSSGSDSSKAAPGPGPSTFSFVPPSKASLAPTPAASPVAPSAASFSFGSSGFKPTLESTPVPSVSAPNIAVKPSFPPSTSAVKVNLSEKFTAAATSTPVSSSQSAPSMPPFSSASKPATSGPLSHPTPLSASPSSVPLKSSVLPSPSAGRSAQGSPSPVPSMVQKSPRITPPVAKPGSPQAKSLQPAVAEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKARTSKACFQVGTSEEMKMLRTESDDLHTFLLEIKETTESLHGDISSLKTTLLEGFAGVEEAREQNERNRDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDRHLEQKKKQRHLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYNQTSPWSLPSAVPSQSSIHSFDSDLESLRNALLKTTIESHTKSLPKVPAKLSPMKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEVSSTSSVSQSLESEDARTSYKDEEAVVQAPRHAPVVRTPSVQPSLLPHAAPFAKSHLVHGSSPGVMGTSVATSASKIIPQGADSTMLATKTVKHGAPSPSHPISAPQAAAAAALRRQMASQAPAVNTLTESTLKNVPQVVNVQELKNNPATPSTAMGSSVAYSAAKTPHPVLTPVAANQAKQGSLINPLKPSGPTPASGQLSSGDKASGTAKIETAVTSTPSASGQFSKPFSFSPSGTGFNFGIITPTPSSNFTAAQGATPSTKESSQLDAFSFGGGSKPSYEAIPESSPPSGITSASNTTPGEPATSSSRPVAPSGTALSTTSSKLETPPSKLGELLFPSSLAGETLGSFSGLRVGQADDSTKPTNKASSTSLTSTQPTKTSGVPSGFNFTAPPVLGKQTEPPVTSSATTTSVAPPAATSTSSTGVFGSLPVTSAGSSGVISFGGTSLSGGKTSFSFGSQQTNSTVPPSAPPPTTAATPLPTSFPTLSFGSLLSSATTPSLPMSSGRSTEEATSSALPEKPGDSEVSASAASLLEEQQSAQLPQAPPQTSDSVKKEPALAQPAVSNSGTAASSTSLVALSAEATPTTTGVPDARSEAVPPASSFSVPGQTAVTAAAISSAGPVAVETSSAPITSSTTSVVAPGPSAEAAAFGTVTSGSSVFAQPPAASSSSAFNQLTNNTATATSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGFSSPAFGTTAPGVFGQTTFGQASVFGQSASSAASGFSFSQPGFSSVPAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSANTGGGLFGQSNAPAFGQSPGFGQGGSVFGGTSAATTTAVSSGFSFCQASGFGSSNTGSVFGQAASTGGIVFGQQSSSSSGSVFGSGNTGRGGGFFSGLGGKPSQDAANKNPFSSASGGFGSTATSNTSNLFGNSGAKTFGGFASSSFGEQKPTGTFSSGGGSVASQGFGFSSPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSNTTSFGTLASQNAPTFGSLSQQTSGFGTQSSGFSGFGSGTGGFSFGSNNSSVQGFGGWRS

Target ORF information:

RefSeq Version	XM_024252122.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii nucleoporin 214 (NUP214), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024252122.1

ORF Insert Sequence:

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTCGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTTGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAATAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGTGTGC ATGATGTCCG GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGGTTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCAATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTC ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGATTAA TTGGGAATCT 
TGGCTACTGG AGGATTCTAG TCGAGCTGAA TTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GAGTTGCCGT AGACTATACG AACCAAGTGG AAATCACCAT CAGTGATGAA 
AAGACTCTTC CTCCTGCTCC AGTTCTCATG TTACTTTCAA CAGATGGTGT GCTTTGTCCA 
TTTTATATGA TTAATCAAAA TCCTGGGGTT AAGTCTCTCA TCAAAACACC AGAGCGACTT 
TCATTAGAAG GAGAGCGACA GCCCAAGTCA CCAGGAAGTA CTCCCACTAC CCCGACCTCC 
TCTCAAGCCC CGCAGAAACT GGATGCTTCT GCAGCTGCAG CCCCTGCCTC TCTTCCACCT 
TCATCACCCG CTGCTCCCAT TGCCACTTTT TCTTTGCTTC CTGCTGGTGG AGCCCCCACT 
GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG TCATCTGCTG CGGTCACTGG GGAGCCCCCT 
TCATATTCCA GTGGCTCCGA CAGCTCCAAA GCAGCCCCGG GCCCTGGCCC ATCAACCTTC 
TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA GCCCCCACCC CTGCAGCGTC TCCCGTGGCT 
CCATCAGCTG CTTCATTCTC GTTTGGATCA TCTGGTTTTA AGCCTACCCT GGAAAGCACA 
CCAGTGCCAA GTGTGTCTGC TCCAAATATA GCAGTGAAGC CCTCCTTCCC ACCCTCAACC 
TCTGCTGTCA AAGTCAACCT TAGTGAAAAG TTTACTGCCG CAGCTACCTC TACTCCTGTT 
AGTAGCTCCC AGAGCGCACC CTCGATGCCG CCATTCTCTT CTGCCTCCAA GCCAGCTACT 
TCTGGACCGC TCAGCCACCC CACGCCTCTC TCAGCATCAC CTAGTTCCGT GCCATTGAAG 
TCCTCAGTCT TGCCCTCACC ATCAGCAGGA CGATCTGCTC AGGGCAGTCC AAGCCCAGTG 
CCCTCAATGG TACAGAAATC ACCCAGGATA ACCCCTCCAG TGGCAAAGCC AGGCTCTCCC 
CAGGCAAAGT CACTTCAGCC TGCTGTTGCA GAGAAGCAGG GACATCAGTG GAAAGATTCA 
GATCCTGTAA TGGCTGGAAT TGGGGAGGAG ATTGCACACT TTCAGAAGGA GTTGGAAGAG 
TTAAAAGCCC GAACTTCCAA AGCCTGTTTC CAAGTGGGCA CTTCTGAGGA GATGAAGATG 
CTGCGAACAG AATCAGATGA CTTGCATACC TTTCTTTTGG AGATTAAAGA GACCACAGAG 
TCGCTTCATG GAGACATAAG TAGCCTGAAA ACAACTTTAC TTGAGGGCTT TGCTGGTGTT 
GAGGAAGCCA GAGAACAAAA TGAAAGAAAT CGTGACTCTG GTTATCTGCA TTTGCTTTAT 
AAAAGACCAC TGGATCCCAA GAGTGAAGCT CAGCTTCAGG AAATTCGGCG CCTTCATCAG 
TATGTGAAAT TTGCTGTCCA AGATGTGAAT GATGTTCTAG ATTTGGAGTG GGATCGGCAT 
CTGGAACAAA AGAAAAAACA AAGGCACCTG CTTGTGCCAG AGCGAGAGAC ACTGTTTAAC 
ACCCTAGCCA ACAACCGGGA AATCATCAAC CAACAGAGGA AGAGGCTGAA TCACCTGGTG 
GACAGTCTTC AGCAGCTCCG CCTTTACAAC CAGACTTCCC CGTGGAGCCT GCCCTCGGCT 
GTTCCTTCCC AGAGCAGCAT TCACAGTTTT GACAGTGACC TTGAAAGCCT GCGCAATGCT 
TTGTTGAAAA CCACCATAGA ATCTCACACC AAATCCTTGC CCAAAGTACC AGCCAAACTG 
TCCCCCATGA AACAGGCACA ACTGAGAAAC TTCTTGGCCA AGAGGAAGAC CCCACCAGTG 
AGATCCACTG CTCCAGCCAG CCTGTCTCGA TCAGCCTTTC TGTCTCAGAG ATATTATGAA 
GACTTGGATG AAGTCAGCTC AACGTCATCT GTCTCCCAGT CTCTGGAGAG TGAAGATGCA 
CGGACGTCCT ATAAAGATGA GGAGGCAGTG GTTCAGGCCC CTCGGCACGC CCCCGTGGTT 
CGCACTCCTT CCGTCCAGCC CAGTCTCTTG CCCCATGCAG CACCTTTTGC TAAATCTCAC 
CTGGTTCATG GTTCTTCACC TGGTGTGATG GGAACTTCAG TGGCTACATC TGCTAGCAAA 
ATTATTCCTC AAGGGGCCGA TAGCACAATG CTTGCCACGA AAACCGTGAA ACATGGTGCA 
CCTAGTCCTT CCCACCCCAT CTCAGCCCCG CAGGCAGCTG CCGCAGCAGC ACTCAGGCGG 
CAGATGGCCA GTCAGGCACC AGCTGTAAAC ACTTTGACTG AATCAACGTT GAAGAATGTC 
CCTCAAGTGG TAAATGTGCA GGAATTGAAG AATAACCCTG CAACCCCTTC TACAGCCATG 
GGTTCTTCAG TGGCCTACTC CGCAGCCAAA ACACCTCACC CAGTGTTGAC CCCAGTGGCC 
GCTAACCAAG CCAAGCAGGG GTCTCTAATA AATCCCCTTA AGCCATCTGG GCCTACACCA 
GCATCCGGTC AGTTATCATC TGGTGACAAA GCTTCAGGGA CAGCCAAGAT AGAAACAGCT 
GTGACTTCGA CCCCATCTGC TTCTGGGCAG TTCAGCAAGC CTTTCTCATT TTCTCCATCA 
GGGACTGGCT TTAATTTTGG GATAATCACA CCAACACCGT CTTCTAATTT CACTGCTGCA 
CAAGGGGCAA CACCCTCCAC TAAAGAGTCA AGCCAGCTGG ATGCATTCTC ATTTGGTGGG 
GGAAGCAAAC CTTCTTATGA GGCCATTCCT GAAAGCTCAC CTCCCTCAGG AATCACATCC 
GCATCAAACA CCACCCCAGG AGAACCTGCC ACATCTAGCA GCAGACCTGT GGCACCTTCT 
GGAACTGCTC TTTCCACTAC CTCTAGTAAG CTGGAAACCC CACCGTCCAA GCTGGGAGAG 
CTTCTGTTTC CAAGTTCTTT GGCTGGAGAG ACTCTGGGAA GTTTTTCAGG ACTGCGTGTT 
GGCCAAGCAG ATGATTCTAC AAAGCCAACC AATAAGGCTT CATCCACAAG CCTAACTAGT 
ACCCAGCCAA CCAAGACGTC AGGCGTGCCC TCAGGGTTTA ATTTTACTGC CCCCCCAGTG 
TTAGGGAAGC AAACGGAGCC CCCTGTGACC TCCTCTGCAA CCACCACCTC AGTAGCACCA 
CCAGCAGCCA CCAGCACTTC CTCAACTGGC GTTTTTGGCA GTCTGCCGGT CACCAGTGCA 
GGATCCTCTG GGGTCATCAG TTTTGGTGGG ACATCTCTAA GTGGTGGCAA GACTAGTTTT 
TCATTTGGAA GCCAACAGAC CAATAGCACA GTGCCCCCAT CTGCCCCACC GCCTACTACA 
GCTGCCACTC CCCTTCCAAC ATCATTCCCC ACATTGTCGT TCGGTAGCCT CCTGAGTTCA 
GCAACTACCC CCTCCCTGCC TATGTCCTCT GGCAGAAGCA CAGAAGAGGC CACTTCATCA 
GCTTTGCCTG AGAAGCCAGG TGACAGTGAG GTCTCAGCAT CAGCAGCCTC ACTTCTAGAG 
GAGCAACAGT CAGCCCAGCT TCCCCAGGCT CCTCCGCAAA CTTCTGACTC TGTTAAAAAA 
GAACCTGCTC TTGCCCAGCC TGCAGTCAGC AACTCTGGCA CTGCAGCATC TAGTACTAGT 
CTTGTAGCAC TTTCTGCAGA GGCTACCCCA ACCACCACCG GGGTCCCTGA TGCCAGGTCG 
GAGGCAGTAC CACCTGCTTC CTCCTTTTCT GTGCCTGGGC AGACTGCTGT CACAGCAGCT 
GCTATCTCAA GTGCAGGCCC TGTGGCCGTC GAAACATCAA GTGCCCCCAT AACCTCCAGC 
ACCACGTCCG TTGTTGCTCC CGGCCCATCT GCAGAGGCAG CAGCATTTGG TACCGTCACT 
TCTGGCTCAT CAGTCTTTGC TCAGCCCCCT GCTGCCAGTT CTAGCTCAGC TTTCAACCAG 
CTCACCAACA ACACAGCCAC TGCCACTTCT GCCACGCCCG TGTTTGGGCA AGTGGCAGCC 
AGCACTGCAC CAAGTCTGTT TGGGCAGCAG ACTGGTAGCA CAGCCAGCAC AGCAGCTGCC 
ACACCACAGG TCAGCAGCTC AGGGTTTAGC AGCCCAGCTT TTGGCACCAC AGCCCCAGGG 
GTCTTTGGAC AGACAACCTT CGGGCAGGCC TCAGTGTTTG GGCAGTCAGC GAGCAGTGCC 
GCAAGTGGCT TTTCCTTCAG TCAGCCTGGG TTCAGTTCCG TGCCTGCCTT CGGTCAGCCT 
GCTTCCTCCA CCCCCACATC CACCAGTGGA AGTGTCTTTG GTGCCGCCTC AAGTACCAGT 
AGCTCCAGTT CCTTCTCATT TGGACAGTCT TCTGCCAACA CAGGAGGGGG GCTGTTTGGC 
CAAAGCAACG CTCCTGCTTT TGGGCAGAGT CCTGGCTTTG GACAGGGAGG CTCTGTATTT 
GGTGGTACCT CAGCTGCCAC CACAACAGCA GTATCCTCTG GGTTCAGCTT TTGTCAAGCT 
TCAGGTTTTG GGTCTAGTAA TACTGGTTCT GTGTTTGGTC AAGCAGCCAG TACTGGTGGA 
ATAGTCTTTG GCCAGCAGTC ATCGTCTTCC AGTGGTAGCG TGTTTGGGTC TGGAAACACT 
GGAAGAGGGG GAGGTTTCTT CAGTGGCCTT GGAGGAAAAC CCAGTCAGGA TGCAGCCAAC 
AAAAACCCAT TCAGCTCGGC CAGTGGGGGC TTTGGATCCA CAGCTACCTC AAATACCTCT 
AACCTATTTG GAAACAGTGG GGCCAAGACA TTTGGTGGAT TTGCCAGCTC GTCGTTTGGA 
GAGCAGAAAC CCACTGGCAC TTTCAGCTCT GGAGGAGGAA GTGTGGCATC CCAAGGCTTT 
GGGTTTTCCT CTCCAAACAA AACAGGTGGC TTCGGTGCTG CTCCAGTGTT TGGCAGCCCT 
CCTACTTTTG GGGGATCCCC TGGGTTTGGA GGGGTGCCAG CATTCGGTTC AGCCCCAGCC 
TTTACAAGCC CTCTGGGCTC GACGGGAGGC AAAGTGTTCG GAGAGGGCAC TGCGGCTGCC 
AGCGCAGGAG GATTCGGGTT TGGGAGCAGC AGCAACACCA CATCCTTCGG CACGCTCGCG 
AGTCAGAATG CCCCCACTTT CGGATCACTG TCCCAACAGA CTTCTGGTTT TGGGACCCAG 
AGTAGCGGAT TCTCTGGTTT TGGATCAGGC ACAGGAGGGT TCAGCTTTGG ATCAAATAAC 
TCATCTGTCC AGGGTTTTGG TGGCTGGCGA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( XM_024252123.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024107891.1 Pongo abelii nucleoporin 214 (NUP214), transcript variant X2, mRNA.

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CloneID	OSa186577
Clone ID Related Accession (Same CDS sequence)	XM_024252123.1
Accession Version	XM_024252123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	nuclear pore complex protein Nup214 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036912.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTCGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTTGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAATAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGTGTGC ATGATGTCCG GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGGTTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCAATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTC ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGATTAA TTGGGAATCT 
TGGCTACTGG AGGATTCTAG TCGAGCTGAA TTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GAGTTGCCGT AGACTATACG AACCAAGTGG AAATCACCAT CAGTGATGAA 
AAGACTCTTC CTCCTGCTCC AGTTCTCATG TTACTTTCAA CAGATGGTGT GCTTTGTCCA 
TTTTATATGA TTAATCAAAA TCCTGGGGTT AAGTCTCTCA TCAAAACACC AGAGCGACTT 
TCATTAGAAG GAGAGCGACA GCCCAAGTCA CCAGGAAGTA CTCCCACTAC CCCGACCTCC 
TCTCAAGCCC CGCAGAAACT GGATGCTTCT GCAGCTGCAG CCCCTGCCTC TCTTCCACCT 
TCATCACCCG CTGCTCCCAT TGCCACTTTT TCTTTGCTTC CTGCTGGTGG AGCCCCCACT 
GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG TCATCTGCTG CGGTCACTGG GGAGCCCCCT 
TCATATTCCA GTGGCTCCGA CAGCTCCAAA GCAGCCCCGG GCCCTGGCCC ATCAACCTTC 
TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA GCCCCCACCC CTGCAGCGTC TCCCGTGGCT 
CCATCAGCTG CTTCATTCTC GTTTGGATCA TCTGGTTTTA AGCCTACCCT GGAAAGCACA 
CCAGTGCCAA GTGTGTCTGC TCCAAATATA GCAGTGAAGC CCTCCTTCCC ACCCTCAACC 
TCTGCTGTCA AAGTCAACCT TAGTGAAAAG TTTACTGCCG CAGCTACCTC TACTCCTGTT 
AGTAGCTCCC AGAGCGCACC CTCGATGCCG CCATTCTCTT CTGCCTCCAA GCCAGCTACT 
TCTGGACCGC TCAGCCACCC CACGCCTCTC TCAGCATCAC CTAGTTCCGT GCCATTGAAG 
TCCTCAGTCT TGCCCTCACC ATCAGGACGA TCTGCTCAGG GCAGTCCAAG CCCAGTGCCC 
TCAATGGTAC AGAAATCACC CAGGATAACC CCTCCAGTGG CAAAGCCAGG CTCTCCCCAG 
GCAAAGTCAC TTCAGCCTGC TGTTGCAGAG AAGCAGGGAC ATCAGTGGAA AGATTCAGAT 
CCTGTAATGG CTGGAATTGG GGAGGAGATT GCACACTTTC AGAAGGAGTT GGAAGAGTTA 
AAAGCCCGAA CTTCCAAAGC CTGTTTCCAA GTGGGCACTT CTGAGGAGAT GAAGATGCTG 
CGAACAGAAT CAGATGACTT GCATACCTTT CTTTTGGAGA TTAAAGAGAC CACAGAGTCG 
CTTCATGGAG ACATAAGTAG CCTGAAAACA ACTTTACTTG AGGGCTTTGC TGGTGTTGAG 
GAAGCCAGAG AACAAAATGA AAGAAATCGT GACTCTGGTT ATCTGCATTT GCTTTATAAA 
AGACCACTGG ATCCCAAGAG TGAAGCTCAG CTTCAGGAAA TTCGGCGCCT TCATCAGTAT 
GTGAAATTTG CTGTCCAAGA TGTGAATGAT GTTCTAGATT TGGAGTGGGA TCGGCATCTG 
GAACAAAAGA AAAAACAAAG GCACCTGCTT GTGCCAGAGC GAGAGACACT GTTTAACACC 
CTAGCCAACA ACCGGGAAAT CATCAACCAA CAGAGGAAGA GGCTGAATCA CCTGGTGGAC 
AGTCTTCAGC AGCTCCGCCT TTACAACCAG ACTTCCCCGT GGAGCCTGCC CTCGGCTGTT 
CCTTCCCAGA GCAGCATTCA CAGTTTTGAC AGTGACCTTG AAAGCCTGCG CAATGCTTTG 
TTGAAAACCA CCATAGAATC TCACACCAAA TCCTTGCCCA AAGTACCAGC CAAACTGTCC 
CCCATGAAAC AGGCACAACT GAGAAACTTC TTGGCCAAGA GGAAGACCCC ACCAGTGAGA 
TCCACTGCTC CAGCCAGCCT GTCTCGATCA GCCTTTCTGT CTCAGAGATA TTATGAAGAC 
TTGGATGAAG TCAGCTCAAC GTCATCTGTC TCCCAGTCTC TGGAGAGTGA AGATGCACGG 
ACGTCCTATA AAGATGAGGA GGCAGTGGTT CAGGCCCCTC GGCACGCCCC CGTGGTTCGC 
ACTCCTTCCG TCCAGCCCAG TCTCTTGCCC CATGCAGCAC CTTTTGCTAA ATCTCACCTG 
GTTCATGGTT CTTCACCTGG TGTGATGGGA ACTTCAGTGG CTACATCTGC TAGCAAAATT 
ATTCCTCAAG GGGCCGATAG CACAATGCTT GCCACGAAAA CCGTGAAACA TGGTGCACCT 
AGTCCTTCCC ACCCCATCTC AGCCCCGCAG GCAGCTGCCG CAGCAGCACT CAGGCGGCAG 
ATGGCCAGTC AGGCACCAGC TGTAAACACT TTGACTGAAT CAACGTTGAA GAATGTCCCT 
CAAGTGGTAA ATGTGCAGGA ATTGAAGAAT AACCCTGCAA CCCCTTCTAC AGCCATGGGT 
TCTTCAGTGG CCTACTCCGC AGCCAAAACA CCTCACCCAG TGTTGACCCC AGTGGCCGCT 
AACCAAGCCA AGCAGGGGTC TCTAATAAAT CCCCTTAAGC CATCTGGGCC TACACCAGCA 
TCCGGTCAGT TATCATCTGG TGACAAAGCT TCAGGGACAG CCAAGATAGA AACAGCTGTG 
ACTTCGACCC CATCTGCTTC TGGGCAGTTC AGCAAGCCTT TCTCATTTTC TCCATCAGGG 
ACTGGCTTTA ATTTTGGGAT AATCACACCA ACACCGTCTT CTAATTTCAC TGCTGCACAA 
GGGGCAACAC CCTCCACTAA AGAGTCAAGC CAGCTGGATG CATTCTCATT TGGTGGGGGA 
AGCAAACCTT CTTATGAGGC CATTCCTGAA AGCTCACCTC CCTCAGGAAT CACATCCGCA 
TCAAACACCA CCCCAGGAGA ACCTGCCACA TCTAGCAGCA GACCTGTGGC ACCTTCTGGA 
ACTGCTCTTT CCACTACCTC TAGTAAGCTG GAAACCCCAC CGTCCAAGCT GGGAGAGCTT 
CTGTTTCCAA GTTCTTTGGC TGGAGAGACT CTGGGAAGTT TTTCAGGACT GCGTGTTGGC 
CAAGCAGATG ATTCTACAAA GCCAACCAAT AAGGCTTCAT CCACAAGCCT AACTAGTACC 
CAGCCAACCA AGACGTCAGG CGTGCCCTCA GGGTTTAATT TTACTGCCCC CCCAGTGTTA 
GGGAAGCAAA CGGAGCCCCC TGTGACCTCC TCTGCAACCA CCACCTCAGT AGCACCACCA 
GCAGCCACCA GCACTTCCTC AACTGGCGTT TTTGGCAGTC TGCCGGTCAC CAGTGCAGGA 
TCCTCTGGGG TCATCAGTTT TGGTGGGACA TCTCTAAGTG GTGGCAAGAC TAGTTTTTCA 
TTTGGAAGCC AACAGACCAA TAGCACAGTG CCCCCATCTG CCCCACCGCC TACTACAGCT 
GCCACTCCCC TTCCAACATC ATTCCCCACA TTGTCGTTCG GTAGCCTCCT GAGTTCAGCA 
ACTACCCCCT CCCTGCCTAT GTCCTCTGGC AGAAGCACAG AAGAGGCCAC TTCATCAGCT 
TTGCCTGAGA AGCCAGGTGA CAGTGAGGTC TCAGCATCAG CAGCCTCACT TCTAGAGGAG 
CAACAGTCAG CCCAGCTTCC CCAGGCTCCT CCGCAAACTT CTGACTCTGT TAAAAAAGAA 
CCTGCTCTTG CCCAGCCTGC AGTCAGCAAC TCTGGCACTG CAGCATCTAG TACTAGTCTT 
GTAGCACTTT CTGCAGAGGC TACCCCAACC ACCACCGGGG TCCCTGATGC CAGGTCGGAG 
GCAGTACCAC CTGCTTCCTC CTTTTCTGTG CCTGGGCAGA CTGCTGTCAC AGCAGCTGCT 
ATCTCAAGTG CAGGCCCTGT GGCCGTCGAA ACATCAAGTG CCCCCATAAC CTCCAGCACC 
ACGTCCGTTG TTGCTCCCGG CCCATCTGCA GAGGCAGCAG CATTTGGTAC CGTCACTTCT 
GGCTCATCAG TCTTTGCTCA GCCCCCTGCT GCCAGTTCTA GCTCAGCTTT CAACCAGCTC 
ACCAACAACA CAGCCACTGC CACTTCTGCC ACGCCCGTGT TTGGGCAAGT GGCAGCCAGC 
ACTGCACCAA GTCTGTTTGG GCAGCAGACT GGTAGCACAG CCAGCACAGC AGCTGCCACA 
CCACAGGTCA GCAGCTCAGG GTTTAGCAGC CCAGCTTTTG GCACCACAGC CCCAGGGGTC 
TTTGGACAGA CAACCTTCGG GCAGGCCTCA GTGTTTGGGC AGTCAGCGAG CAGTGCCGCA 
AGTGGCTTTT CCTTCAGTCA GCCTGGGTTC AGTTCCGTGC CTGCCTTCGG TCAGCCTGCT 
TCCTCCACCC CCACATCCAC CAGTGGAAGT GTCTTTGGTG CCGCCTCAAG TACCAGTAGC 
TCCAGTTCCT TCTCATTTGG ACAGTCTTCT GCCAACACAG GAGGGGGGCT GTTTGGCCAA 
AGCAACGCTC CTGCTTTTGG GCAGAGTCCT GGCTTTGGAC AGGGAGGCTC TGTATTTGGT 
GGTACCTCAG CTGCCACCAC AACAGCAGTA TCCTCTGGGT TCAGCTTTTG TCAAGCTTCA 
GGTTTTGGGT CTAGTAATAC TGGTTCTGTG TTTGGTCAAG CAGCCAGTAC TGGTGGAATA 
GTCTTTGGCC AGCAGTCATC GTCTTCCAGT GGTAGCGTGT TTGGGTCTGG AAACACTGGA 
AGAGGGGGAG GTTTCTTCAG TGGCCTTGGA GGAAAACCCA GTCAGGATGC AGCCAACAAA 
AACCCATTCA GCTCGGCCAG TGGGGGCTTT GGATCCACAG CTACCTCAAA TACCTCTAAC 
CTATTTGGAA ACAGTGGGGC CAAGACATTT GGTGGATTTG CCAGCTCGTC GTTTGGAGAG 
CAGAAACCCA CTGGCACTTT CAGCTCTGGA GGAGGAAGTG TGGCATCCCA AGGCTTTGGG 
TTTTCCTCTC CAAACAAAAC AGGTGGCTTC GGTGCTGCTC CAGTGTTTGG CAGCCCTCCT 
ACTTTTGGGG GATCCCCTGG GTTTGGAGGG GTGCCAGCAT TCGGTTCAGC CCCAGCCTTT 
ACAAGCCCTC TGGGCTCGAC GGGAGGCAAA GTGTTCGGAG AGGGCACTGC GGCTGCCAGC 
GCAGGAGGAT TCGGGTTTGG GAGCAGCAGC AACACCACAT CCTTCGGCAC GCTCGCGAGT 
CAGAATGCCC CCACTTTCGG ATCACTGTCC CAACAGACTT CTGGTTTTGG GACCCAGAGT 
AGCGGATTCT CTGGTTTTGG ATCAGGCACA GGAGGGTTCA GCTTTGGATC AAATAACTCA 
TCTGTCCAGG GTTTTGGTGG CTGGCGAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024107891.1
CDS	139..6411
Translation	MGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSVCMMSGEYGSIIAFFDVRTFSNEAKQQKRPFAYHKLLKDAGGMVVDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQINWESWLLEDSSRAELPVTDKSDDSLPMGVAVDYTNQVEITISDEKTLPPAPVLMLLSTDGVLCPFYMINQNPGVKSLIKTPERLSLEGERQPKSPGSTPTTPTSSQAPQKLDASAAAAPASLPPSSPAAPIATFSLLPAGGAPTVFSFGSSSLKSSAAVTGEPPSYSSGSDSSKAAPGPGPSTFSFVPPSKASLAPTPAASPVAPSAASFSFGSSGFKPTLESTPVPSVSAPNIAVKPSFPPSTSAVKVNLSEKFTAAATSTPVSSSQSAPSMPPFSSASKPATSGPLSHPTPLSASPSSVPLKSSVLPSPSGRSAQGSPSPVPSMVQKSPRITPPVAKPGSPQAKSLQPAVAEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKARTSKACFQVGTSEEMKMLRTESDDLHTFLLEIKETTESLHGDISSLKTTLLEGFAGVEEAREQNERNRDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDRHLEQKKKQRHLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYNQTSPWSLPSAVPSQSSIHSFDSDLESLRNALLKTTIESHTKSLPKVPAKLSPMKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEVSSTSSVSQSLESEDARTSYKDEEAVVQAPRHAPVVRTPSVQPSLLPHAAPFAKSHLVHGSSPGVMGTSVATSASKIIPQGADSTMLATKTVKHGAPSPSHPISAPQAAAAAALRRQMASQAPAVNTLTESTLKNVPQVVNVQELKNNPATPSTAMGSSVAYSAAKTPHPVLTPVAANQAKQGSLINPLKPSGPTPASGQLSSGDKASGTAKIETAVTSTPSASGQFSKPFSFSPSGTGFNFGIITPTPSSNFTAAQGATPSTKESSQLDAFSFGGGSKPSYEAIPESSPPSGITSASNTTPGEPATSSSRPVAPSGTALSTTSSKLETPPSKLGELLFPSSLAGETLGSFSGLRVGQADDSTKPTNKASSTSLTSTQPTKTSGVPSGFNFTAPPVLGKQTEPPVTSSATTTSVAPPAATSTSSTGVFGSLPVTSAGSSGVISFGGTSLSGGKTSFSFGSQQTNSTVPPSAPPPTTAATPLPTSFPTLSFGSLLSSATTPSLPMSSGRSTEEATSSALPEKPGDSEVSASAASLLEEQQSAQLPQAPPQTSDSVKKEPALAQPAVSNSGTAASSTSLVALSAEATPTTTGVPDARSEAVPPASSFSVPGQTAVTAAAISSAGPVAVETSSAPITSSTTSVVAPGPSAEAAAFGTVTSGSSVFAQPPAASSSSAFNQLTNNTATATSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGFSSPAFGTTAPGVFGQTTFGQASVFGQSASSAASGFSFSQPGFSSVPAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSANTGGGLFGQSNAPAFGQSPGFGQGGSVFGGTSAATTTAVSSGFSFCQASGFGSSNTGSVFGQAASTGGIVFGQQSSSSSGSVFGSGNTGRGGGFFSGLGGKPSQDAANKNPFSSASGGFGSTATSNTSNLFGNSGAKTFGGFASSSFGEQKPTGTFSSGGGSVASQGFGFSSPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSNTTSFGTLASQNAPTFGSLSQQTSGFGTQSSGFSGFGSGTGGFSFGSNNSSVQGFGGWRS

Target ORF information:

RefSeq Version	XM_024252123.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii nucleoporin 214 (NUP214), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024252123.1

ORF Insert Sequence:

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTCGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTTGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAATAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGTGTGC ATGATGTCCG GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGGTTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCAATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTC ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGATTAA TTGGGAATCT 
TGGCTACTGG AGGATTCTAG TCGAGCTGAA TTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GAGTTGCCGT AGACTATACG AACCAAGTGG AAATCACCAT CAGTGATGAA 
AAGACTCTTC CTCCTGCTCC AGTTCTCATG TTACTTTCAA CAGATGGTGT GCTTTGTCCA 
TTTTATATGA TTAATCAAAA TCCTGGGGTT AAGTCTCTCA TCAAAACACC AGAGCGACTT 
TCATTAGAAG GAGAGCGACA GCCCAAGTCA CCAGGAAGTA CTCCCACTAC CCCGACCTCC 
TCTCAAGCCC CGCAGAAACT GGATGCTTCT GCAGCTGCAG CCCCTGCCTC TCTTCCACCT 
TCATCACCCG CTGCTCCCAT TGCCACTTTT TCTTTGCTTC CTGCTGGTGG AGCCCCCACT 
GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG TCATCTGCTG CGGTCACTGG GGAGCCCCCT 
TCATATTCCA GTGGCTCCGA CAGCTCCAAA GCAGCCCCGG GCCCTGGCCC ATCAACCTTC 
TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA GCCCCCACCC CTGCAGCGTC TCCCGTGGCT 
CCATCAGCTG CTTCATTCTC GTTTGGATCA TCTGGTTTTA AGCCTACCCT GGAAAGCACA 
CCAGTGCCAA GTGTGTCTGC TCCAAATATA GCAGTGAAGC CCTCCTTCCC ACCCTCAACC 
TCTGCTGTCA AAGTCAACCT TAGTGAAAAG TTTACTGCCG CAGCTACCTC TACTCCTGTT 
AGTAGCTCCC AGAGCGCACC CTCGATGCCG CCATTCTCTT CTGCCTCCAA GCCAGCTACT 
TCTGGACCGC TCAGCCACCC CACGCCTCTC TCAGCATCAC CTAGTTCCGT GCCATTGAAG 
TCCTCAGTCT TGCCCTCACC ATCAGGACGA TCTGCTCAGG GCAGTCCAAG CCCAGTGCCC 
TCAATGGTAC AGAAATCACC CAGGATAACC CCTCCAGTGG CAAAGCCAGG CTCTCCCCAG 
GCAAAGTCAC TTCAGCCTGC TGTTGCAGAG AAGCAGGGAC ATCAGTGGAA AGATTCAGAT 
CCTGTAATGG CTGGAATTGG GGAGGAGATT GCACACTTTC AGAAGGAGTT GGAAGAGTTA 
AAAGCCCGAA CTTCCAAAGC CTGTTTCCAA GTGGGCACTT CTGAGGAGAT GAAGATGCTG 
CGAACAGAAT CAGATGACTT GCATACCTTT CTTTTGGAGA TTAAAGAGAC CACAGAGTCG 
CTTCATGGAG ACATAAGTAG CCTGAAAACA ACTTTACTTG AGGGCTTTGC TGGTGTTGAG 
GAAGCCAGAG AACAAAATGA AAGAAATCGT GACTCTGGTT ATCTGCATTT GCTTTATAAA 
AGACCACTGG ATCCCAAGAG TGAAGCTCAG CTTCAGGAAA TTCGGCGCCT TCATCAGTAT 
GTGAAATTTG CTGTCCAAGA TGTGAATGAT GTTCTAGATT TGGAGTGGGA TCGGCATCTG 
GAACAAAAGA AAAAACAAAG GCACCTGCTT GTGCCAGAGC GAGAGACACT GTTTAACACC 
CTAGCCAACA ACCGGGAAAT CATCAACCAA CAGAGGAAGA GGCTGAATCA CCTGGTGGAC 
AGTCTTCAGC AGCTCCGCCT TTACAACCAG ACTTCCCCGT GGAGCCTGCC CTCGGCTGTT 
CCTTCCCAGA GCAGCATTCA CAGTTTTGAC AGTGACCTTG AAAGCCTGCG CAATGCTTTG 
TTGAAAACCA CCATAGAATC TCACACCAAA TCCTTGCCCA AAGTACCAGC CAAACTGTCC 
CCCATGAAAC AGGCACAACT GAGAAACTTC TTGGCCAAGA GGAAGACCCC ACCAGTGAGA 
TCCACTGCTC CAGCCAGCCT GTCTCGATCA GCCTTTCTGT CTCAGAGATA TTATGAAGAC 
TTGGATGAAG TCAGCTCAAC GTCATCTGTC TCCCAGTCTC TGGAGAGTGA AGATGCACGG 
ACGTCCTATA AAGATGAGGA GGCAGTGGTT CAGGCCCCTC GGCACGCCCC CGTGGTTCGC 
ACTCCTTCCG TCCAGCCCAG TCTCTTGCCC CATGCAGCAC CTTTTGCTAA ATCTCACCTG 
GTTCATGGTT CTTCACCTGG TGTGATGGGA ACTTCAGTGG CTACATCTGC TAGCAAAATT 
ATTCCTCAAG GGGCCGATAG CACAATGCTT GCCACGAAAA CCGTGAAACA TGGTGCACCT 
AGTCCTTCCC ACCCCATCTC AGCCCCGCAG GCAGCTGCCG CAGCAGCACT CAGGCGGCAG 
ATGGCCAGTC AGGCACCAGC TGTAAACACT TTGACTGAAT CAACGTTGAA GAATGTCCCT 
CAAGTGGTAA ATGTGCAGGA ATTGAAGAAT AACCCTGCAA CCCCTTCTAC AGCCATGGGT 
TCTTCAGTGG CCTACTCCGC AGCCAAAACA CCTCACCCAG TGTTGACCCC AGTGGCCGCT 
AACCAAGCCA AGCAGGGGTC TCTAATAAAT CCCCTTAAGC CATCTGGGCC TACACCAGCA 
TCCGGTCAGT TATCATCTGG TGACAAAGCT TCAGGGACAG CCAAGATAGA AACAGCTGTG 
ACTTCGACCC CATCTGCTTC TGGGCAGTTC AGCAAGCCTT TCTCATTTTC TCCATCAGGG 
ACTGGCTTTA ATTTTGGGAT AATCACACCA ACACCGTCTT CTAATTTCAC TGCTGCACAA 
GGGGCAACAC CCTCCACTAA AGAGTCAAGC CAGCTGGATG CATTCTCATT TGGTGGGGGA 
AGCAAACCTT CTTATGAGGC CATTCCTGAA AGCTCACCTC CCTCAGGAAT CACATCCGCA 
TCAAACACCA CCCCAGGAGA ACCTGCCACA TCTAGCAGCA GACCTGTGGC ACCTTCTGGA 
ACTGCTCTTT CCACTACCTC TAGTAAGCTG GAAACCCCAC CGTCCAAGCT GGGAGAGCTT 
CTGTTTCCAA GTTCTTTGGC TGGAGAGACT CTGGGAAGTT TTTCAGGACT GCGTGTTGGC 
CAAGCAGATG ATTCTACAAA GCCAACCAAT AAGGCTTCAT CCACAAGCCT AACTAGTACC 
CAGCCAACCA AGACGTCAGG CGTGCCCTCA GGGTTTAATT TTACTGCCCC CCCAGTGTTA 
GGGAAGCAAA CGGAGCCCCC TGTGACCTCC TCTGCAACCA CCACCTCAGT AGCACCACCA 
GCAGCCACCA GCACTTCCTC AACTGGCGTT TTTGGCAGTC TGCCGGTCAC CAGTGCAGGA 
TCCTCTGGGG TCATCAGTTT TGGTGGGACA TCTCTAAGTG GTGGCAAGAC TAGTTTTTCA 
TTTGGAAGCC AACAGACCAA TAGCACAGTG CCCCCATCTG CCCCACCGCC TACTACAGCT 
GCCACTCCCC TTCCAACATC ATTCCCCACA TTGTCGTTCG GTAGCCTCCT GAGTTCAGCA 
ACTACCCCCT CCCTGCCTAT GTCCTCTGGC AGAAGCACAG AAGAGGCCAC TTCATCAGCT 
TTGCCTGAGA AGCCAGGTGA CAGTGAGGTC TCAGCATCAG CAGCCTCACT TCTAGAGGAG 
CAACAGTCAG CCCAGCTTCC CCAGGCTCCT CCGCAAACTT CTGACTCTGT TAAAAAAGAA 
CCTGCTCTTG CCCAGCCTGC AGTCAGCAAC TCTGGCACTG CAGCATCTAG TACTAGTCTT 
GTAGCACTTT CTGCAGAGGC TACCCCAACC ACCACCGGGG TCCCTGATGC CAGGTCGGAG 
GCAGTACCAC CTGCTTCCTC CTTTTCTGTG CCTGGGCAGA CTGCTGTCAC AGCAGCTGCT 
ATCTCAAGTG CAGGCCCTGT GGCCGTCGAA ACATCAAGTG CCCCCATAAC CTCCAGCACC 
ACGTCCGTTG TTGCTCCCGG CCCATCTGCA GAGGCAGCAG CATTTGGTAC CGTCACTTCT 
GGCTCATCAG TCTTTGCTCA GCCCCCTGCT GCCAGTTCTA GCTCAGCTTT CAACCAGCTC 
ACCAACAACA CAGCCACTGC CACTTCTGCC ACGCCCGTGT TTGGGCAAGT GGCAGCCAGC 
ACTGCACCAA GTCTGTTTGG GCAGCAGACT GGTAGCACAG CCAGCACAGC AGCTGCCACA 
CCACAGGTCA GCAGCTCAGG GTTTAGCAGC CCAGCTTTTG GCACCACAGC CCCAGGGGTC 
TTTGGACAGA CAACCTTCGG GCAGGCCTCA GTGTTTGGGC AGTCAGCGAG CAGTGCCGCA 
AGTGGCTTTT CCTTCAGTCA GCCTGGGTTC AGTTCCGTGC CTGCCTTCGG TCAGCCTGCT 
TCCTCCACCC CCACATCCAC CAGTGGAAGT GTCTTTGGTG CCGCCTCAAG TACCAGTAGC 
TCCAGTTCCT TCTCATTTGG ACAGTCTTCT GCCAACACAG GAGGGGGGCT GTTTGGCCAA 
AGCAACGCTC CTGCTTTTGG GCAGAGTCCT GGCTTTGGAC AGGGAGGCTC TGTATTTGGT 
GGTACCTCAG CTGCCACCAC AACAGCAGTA TCCTCTGGGT TCAGCTTTTG TCAAGCTTCA 
GGTTTTGGGT CTAGTAATAC TGGTTCTGTG TTTGGTCAAG CAGCCAGTAC TGGTGGAATA 
GTCTTTGGCC AGCAGTCATC GTCTTCCAGT GGTAGCGTGT TTGGGTCTGG AAACACTGGA 
AGAGGGGGAG GTTTCTTCAG TGGCCTTGGA GGAAAACCCA GTCAGGATGC AGCCAACAAA 
AACCCATTCA GCTCGGCCAG TGGGGGCTTT GGATCCACAG CTACCTCAAA TACCTCTAAC 
CTATTTGGAA ACAGTGGGGC CAAGACATTT GGTGGATTTG CCAGCTCGTC GTTTGGAGAG 
CAGAAACCCA CTGGCACTTT CAGCTCTGGA GGAGGAAGTG TGGCATCCCA AGGCTTTGGG 
TTTTCCTCTC CAAACAAAAC AGGTGGCTTC GGTGCTGCTC CAGTGTTTGG CAGCCCTCCT 
ACTTTTGGGG GATCCCCTGG GTTTGGAGGG GTGCCAGCAT TCGGTTCAGC CCCAGCCTTT 
ACAAGCCCTC TGGGCTCGAC GGGAGGCAAA GTGTTCGGAG AGGGCACTGC GGCTGCCAGC 
GCAGGAGGAT TCGGGTTTGG GAGCAGCAGC AACACCACAT CCTTCGGCAC GCTCGCGAGT 
CAGAATGCCC CCACTTTCGG ATCACTGTCC CAACAGACTT CTGGTTTTGG GACCCAGAGT 
AGCGGATTCT CTGGTTTTGG ATCAGGCACA GGAGGGTTCA GCTTTGGATC AAATAACTCA 
TCTGTCCAGG GTTTTGGTGG CTGGCGAAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( XM_024252124.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024107892.1 Pongo abelii nucleoporin 214 (NUP214), transcript variant X3, mRNA.

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CloneID	OSa186578
Clone ID Related Accession (Same CDS sequence)	XM_024252124.1
Accession Version	XM_024252124.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	nuclear pore complex protein Nup214 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036912.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTCGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTTGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAATAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGTGTGC ATGATGTCCG GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGGTTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCAATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTC ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGATTAA TTGGGAATCT 
TGGCTACTGG AGGATTCTAG TCGAGCTGAA TTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GAGTTGCCGT AGACTATACG AACCAAGTGG AAATCACCAT CAGTGATGAA 
AAGACTCTTC CTCCTGCTCC AGTTCTCATG TTACTTTCAA CAGATGGTGT GCTTTGTCCA 
TTTTATATGA TTAATCAAAA TCCTGGGGTT AAGTCTCTCA TCAAAACACC AGAGCGACTT 
TCATTAGAAG GAGAGCGACA GCCCAAGTCA CCAGGAAGTA CTCCCACTAC CCCGACCTCC 
TCTCAAGCCC CGCAGAAACT GGATGCTTCT GCAGCTGCAG CCCCTGCCTC TCTTCCACCT 
TCATCACCCG CTGCTCCCAT TGCCACTTTT TCTTTGCTTC CTGCTGGTGG AGCCCCCACT 
GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG TCATCTGCTG CGGTCACTGG GGAGCCCCCT 
TCATATTCCA GTGGCTCCGA CAGCTCCAAA GCAGCCCCGG GCCCTGGCCC ATCAACCTTC 
TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA GCCCCCACCC CTGCAGCGTC TCCCGTGGCT 
CCATCAGCTG CTTCATTCTC GTTTGGATCA TCTGGTTTTA AGCCTACCCT GGAAAGCACA 
CCAGTGCCAA GTGTGTCTGC TCCAAATATA GCAGTGAAGC CCTCCTTCCC ACCCTCAACC 
TCTGCTGTCA AAGTCAACCT TAGTGAAAAG TTTACTGCCG CAGCTACCTC TACTCCTGTT 
AGTAGCTCCC AGAGCGCACC CTCGATGCCG CCATTCTCTT CTGCCTCCAA GCCAGCTACT 
TCTGGACCGC TCAGCCACCC CACGCCTCTC TCAGCATCAC CTAGTTCCGT GCCATTGAAG 
TCCTCAGTCT TGCCCTCACC ATCAGCAGGA CGATCTGCTC AGGGCAGTCC AAGCCCAGTG 
CCCTCAATGG TACAGAAATC ACCCAGGATA ACCCCTCCAG TGGCAAAGCC AGGCTCTCCC 
CAGGCAAAGT CACTTCAGCC TGCTGTTGCA GAGAAGCAGG GACATCAGTG GAAAGATTCA 
GATCCTGTAA TGGCTGGAAT TGGGGAGGAG ATTGCACACT TTCAGAAGGA GTTGGAAGAG 
TTAAAAGCCC GAACTTCCAA AGCCTGTTTC CAAGTGGGCA CTTCTGAGGA GATGAAGATG 
CTGCGAACAG AATCAGATGA CTTGCATACC TTTCTTTTGG AGATTAAAGA GACCACAGAG 
TCGCTTCATG GAGACATAAG TAGCCTGAAA ACAACTTTAC TTGAGGGCTT TGCTGGTGTT 
GAGGAAGCCA GAGAACAAAA TGAAAGAAAT CGTGACTCTG GTTATCTGCA TTTGCTTTAT 
AAAAGACCAC TGGATCCCAA GAGTGAAGCT CAGCTTCAGG AAATTCGGCG CCTTCATCAG 
TATGTGAAAT TTGCTGTCCA AGATGTGAAT GATGTTCTAG ATTTGGAGTG GGATCGGCAT 
CTGGAACAAA AGAAAAAACA AAGGCACCTG CTTGTGCCAG AGCGAGAGAC ACTGTTTAAC 
ACCCTAGCCA ACAACCGGGA AATCATCAAC CAACAGAGGA AGAGGCTGAA TCACCTGGTG 
GACAGTCTTC AGCAGCTCCG CCTTTACAAC CAGACTTCCC CGTGGAGCCT GCCCTCGGCT 
GTTCCTTCCC AGAGCAGCAT TCACAGTTTT GACAGTGACC TTGAAAGCCT GCGCAATGCT 
TTGTTGAAAA CCACCATAGA ATCTCACACC AAATCCTTGC CCAAAGTACC AGCCAAACTG 
TCCCCCATGA AACAGGCACA ACTGAGAAAC TTCTTGGCCA AGAGGAAGAC CCCACCAGTG 
AGATCCACTG CTCCAGCCAG CCTGTCTCGA TCAGCCTTTC TGTCTCAGAG ATATTATGAA 
GACTTGGATG AAGTCAGCTC AACGTCATCT GTCTCCCAGT CTCTGGAGAG TGAAGATGCA 
CGGACGTCCT ATAAAGATGA GGAGGCAGTG GTTCAGGCCC CTCGGCACGC CCCCGTGGTT 
CGCACTCCTT CCGTCCAGCC CAGTCTCTTG CCCCATGCAG CACCTTTTGC TAAATCTCAC 
CTGGTTCATG GTTCTTCACC TGGTGTGATG GGAACTTCAG TGGCTACATC TGCTAGCAAA 
ATTATTCCTC AAGGGGCCGA TAGCACAATG CTTGCCACGA AAACCGTGAA ACATGGTGCA 
CCTAGTCCTT CCCACCCCAT CTCAGCCCCG CAGGCAGCTG CCGCAGCAGC ACTCAGGCGG 
CAGATGGCCA GTCAGGCACC AGCTGTAAAC ACTTTGACTG AATCAACGTT GAAGAATGTC 
CCTCAAGTGG TAAATGTGCA GGAATTGAAG AATAACCCTG CAACCCCTTC TACAGCCATG 
GGTTCTTCAG TGGCCTACTC CGCAGCCAAA ACACCTCACC CAGTGTTGAC CCCAGTGGCC 
GCTAACCAAG CCAAGCAGGG GTCTCTAATA AATCCCCTTA AGCCATCTGG GCCTACACCA 
GCATCCGGTC AGTTATCATC TGGTGACAAA GCTTCAGGGA CAGCCAAGAT AGAAACAGCT 
GTGACTTCGA CCCCATCTGC TTCTGGGCAG TTCAGCAAGC CTTTCTCATT TTCTCCATCA 
GGGACTGGCT TTAATTTTGG GATAATCACA CCAACACCGT CTTCTAATTT CACTGCTGCA 
CAAGGGGCAA CACCCTCCAC TAAAGAGTCA AGCCAGCTGG ATGCATTCTC ATTTGGTGGG 
GGAAGCAAAC CTTCTTATGA GGCCATTCCT GAAAGCTCAC CTCCCTCAGG AATCACATCC 
GCATCAAACA CCACCCCAGG AGAACCTGCC ACATCTAGCA GCAGACCTGT GGCACCTTCT 
GGAACTGCTC TTTCCACTAC CTCTAGTAAG CTGGAAACCC CACCGTCCAA GCTGGGAGAG 
CTTCTGTTTC CAAGTTCTTT GGCTGGAGAG ACTCTGGGAA GTTTTTCAGG ACTGCGTGTT 
GGCCAAGCAG ATGATTCTAC AAAGCCAACC AATAAGGCTT CATCCACAAG CCTAACTAGT 
ACCCAGCCAA CCAAGACGTC AGGCGTGCCC TCAGGGTTTA ATTTTACTGC CCCCCCAGTG 
TTAGGGAAGC AAACGGAGCC CCCTGTGACC TCCTCTGCAA CCACCACCTC AGTAGCACCA 
CCAGCAGCCA CCAGCACTTC CTCAACTGGC GTTTTTGGCA GTCTGCCGGT CACCAGTGCA 
GGATCCTCTG GGGTCATCAG TTTTGGTGGG ACATCTCTAA GTGGTGGCAA GACTAGTTTT 
TCATTTGGAA GCCAACAGAC CAATAGCACA GTGCCCCCAT CTGCCCCACC GCCTACTACA 
GCTGCCACTC CCCTTCCAAC ATCATTCCCC ACATTGTCGT TCGGTAGCCT CCTGAGTTCA 
GCAACTACCC CCTCCCTGCC TATGTCCTCT GGCAGAAGCA CAGAAGAGGC CACTTCATCA 
GCTTTGCCTG AGAAGCCAGG TGACAGTGAG GTCTCAGCAT CAGCAGCCTC ACTTCTAGAG 
GAGCAACAGT CAGCCCAGCT TCCCCAGGCT CCTCCGCAAA CTTCTGACTC TGTTAAAAAA 
GAACCTGCTC TTGCCCAGCC TGCAGTCAGC AACTCTGGCA CTGCAGCATC TAGTACTAGT 
CTTGTAGCAC TTTCTGCAGA GGCTACCCCA ACCACCACCG GGGTCCCTGA TGCCAGGTCG 
GAGGCAGTAC CACCTGCTTC CTCCTTTTCT GTGCCTGGGC AGACTGCTGT CACAGCAGCT 
GCTATCTCAA GTGCAGGCCC TGTGGCCGTC GAAACATCAA GTGCCCCCAT AACCTCCAGC 
ACCACGTCCG TTGTTGCTCC CGGCCCATCT GCAGAGGCAG CAGCATTTGG TACCGTCACT 
TCTGGCTCAT CAGTCTTTGC TCAGCCCCCT GCTGCCAGTT CTAGCTCAGC TTTCAACCAG 
CTCACCAACA ACACAGCCAC TGCCACTTCT GCCACGCCCG TGTTTGGGCA AGTGGCAGCC 
AGCACTGCAC CAAGTCTGTT TGGGCAGCAG ACTGGTAGCA CAGCCAGCAC AGCAGCTGCC 
ACACCACAGG TCAGCAGCTC AGGGTTTAGC AGCCCAGCTT TTGGCACCAC AGCCCCAGGG 
GTCTTTGGAC AGACAACCTT CGGGCAGGCC TCAGTGTTTG GGCAGTCAGC GAGCAGTGCC 
GCAAGTGGCT TTTCCTTCAG TCAGCCTGGG TTCAGTTCCG TGCCTGCCTT CGGTCAGCCT 
GCTTCCTCCA CCCCCACATC CACCAGTGGA AGTGTCTTTG GTGCCGCCTC AAGTACCAGT 
AGCTCCAGTT CCTTCTCATT TGGACAGTCT TCTGCCAACA CAGGAGGGGG GCTGTTTGGC 
CAAAGCAACG CTCCTGCTTT TGGGCAGAGT CCTGGCTTTG GACAGGGAGG CTCTGTATTT 
GGTGGTACCT CAGCTGCCAC CACAACAGCA GTATCCTCTG GGTTCAGCTT TTGTCAAGCT 
TCAGGTTTTG GGTCTAGTAA TACTGGTTCT GTGTTTGGTC AAGCAGCCAG TACTGGTGGA 
ATAGTCTTTG GCCAGCAGTC ATCGTCTTCC AGTGGTAGCG TGTTTGGGTC TGGAAACACT 
GGAAGAGGGG GAGGTTTCTT CAGTGGCCTT GGAGGAAAAC CCAGTCAGGA TGCAGCCAAC 
AAAAACCCAT TCAGCTCGGC CAGTGGGGGC TTTGGATCCA CAGCTACCTC AAATACCTCT 
AACCTATTTG GAAACAGTGG GGCCAAGACA TTTGGTGGAT TTGCCAGCTC GTCGTTTGGA 
GAGCAGAAAC CCACTGGCAC TTTCAGCTCT GGAGGAGGAA GTGTGGCATC CCAAGGCTTT 
GGGTTTTCCT CTCCAAACAA AACAGGTGGC TTCGGTGCTG CTCCAGTGTT TGGCAGCCCT 
CCTACTTTTG GGGGATCCCC TGGGTTTGGA GGGGTGCCAG CATTCGGTTC AGCCCCAGCC 
TTTACAAGCC CTCTGGGCTC GACGGGAGGC AAAGTGTTCG GAGAGGGCAC TGCGGCTGCC 
AGCGCAGGAG GATTCGGGTT TGGGAGCAGC AGCAACACCA CATCCTTCGG CACGCTCGCG 
AGTCAGAATG CCCCCACTTT CGGATCACTG TCCCAACAGA CTTCTGGTTT TGGGACCCAG 
AGTAGCGGAT TCTCTGGTTT TGGATCAGGC ACAGGAGGGT TCAGCTTTGG ATCAAATAAC 
TCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024107892.1
CDS	139..6384
Translation	MGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSVCMMSGEYGSIIAFFDVRTFSNEAKQQKRPFAYHKLLKDAGGMVVDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQINWESWLLEDSSRAELPVTDKSDDSLPMGVAVDYTNQVEITISDEKTLPPAPVLMLLSTDGVLCPFYMINQNPGVKSLIKTPERLSLEGERQPKSPGSTPTTPTSSQAPQKLDASAAAAPASLPPSSPAAPIATFSLLPAGGAPTVFSFGSSSLKSSAAVTGEPPSYSSGSDSSKAAPGPGPSTFSFVPPSKASLAPTPAASPVAPSAASFSFGSSGFKPTLESTPVPSVSAPNIAVKPSFPPSTSAVKVNLSEKFTAAATSTPVSSSQSAPSMPPFSSASKPATSGPLSHPTPLSASPSSVPLKSSVLPSPSAGRSAQGSPSPVPSMVQKSPRITPPVAKPGSPQAKSLQPAVAEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKARTSKACFQVGTSEEMKMLRTESDDLHTFLLEIKETTESLHGDISSLKTTLLEGFAGVEEAREQNERNRDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDRHLEQKKKQRHLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYNQTSPWSLPSAVPSQSSIHSFDSDLESLRNALLKTTIESHTKSLPKVPAKLSPMKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEVSSTSSVSQSLESEDARTSYKDEEAVVQAPRHAPVVRTPSVQPSLLPHAAPFAKSHLVHGSSPGVMGTSVATSASKIIPQGADSTMLATKTVKHGAPSPSHPISAPQAAAAAALRRQMASQAPAVNTLTESTLKNVPQVVNVQELKNNPATPSTAMGSSVAYSAAKTPHPVLTPVAANQAKQGSLINPLKPSGPTPASGQLSSGDKASGTAKIETAVTSTPSASGQFSKPFSFSPSGTGFNFGIITPTPSSNFTAAQGATPSTKESSQLDAFSFGGGSKPSYEAIPESSPPSGITSASNTTPGEPATSSSRPVAPSGTALSTTSSKLETPPSKLGELLFPSSLAGETLGSFSGLRVGQADDSTKPTNKASSTSLTSTQPTKTSGVPSGFNFTAPPVLGKQTEPPVTSSATTTSVAPPAATSTSSTGVFGSLPVTSAGSSGVISFGGTSLSGGKTSFSFGSQQTNSTVPPSAPPPTTAATPLPTSFPTLSFGSLLSSATTPSLPMSSGRSTEEATSSALPEKPGDSEVSASAASLLEEQQSAQLPQAPPQTSDSVKKEPALAQPAVSNSGTAASSTSLVALSAEATPTTTGVPDARSEAVPPASSFSVPGQTAVTAAAISSAGPVAVETSSAPITSSTTSVVAPGPSAEAAAFGTVTSGSSVFAQPPAASSSSAFNQLTNNTATATSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGFSSPAFGTTAPGVFGQTTFGQASVFGQSASSAASGFSFSQPGFSSVPAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSANTGGGLFGQSNAPAFGQSPGFGQGGSVFGGTSAATTTAVSSGFSFCQASGFGSSNTGSVFGQAASTGGIVFGQQSSSSSGSVFGSGNTGRGGGFFSGLGGKPSQDAANKNPFSSASGGFGSTATSNTSNLFGNSGAKTFGGFASSSFGEQKPTGTFSSGGGSVASQGFGFSSPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSNTTSFGTLASQNAPTFGSLSQQTSGFGTQSSGFSGFGSGTGGFSFGSNNS

Target ORF information:

RefSeq Version	XM_024252124.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii nucleoporin 214 (NUP214), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024252124.1

ORF Insert Sequence:

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTCGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTTGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAATAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGTGTGC ATGATGTCCG GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGGTTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCAATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTC ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGATTAA TTGGGAATCT 
TGGCTACTGG AGGATTCTAG TCGAGCTGAA TTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GAGTTGCCGT AGACTATACG AACCAAGTGG AAATCACCAT CAGTGATGAA 
AAGACTCTTC CTCCTGCTCC AGTTCTCATG TTACTTTCAA CAGATGGTGT GCTTTGTCCA 
TTTTATATGA TTAATCAAAA TCCTGGGGTT AAGTCTCTCA TCAAAACACC AGAGCGACTT 
TCATTAGAAG GAGAGCGACA GCCCAAGTCA CCAGGAAGTA CTCCCACTAC CCCGACCTCC 
TCTCAAGCCC CGCAGAAACT GGATGCTTCT GCAGCTGCAG CCCCTGCCTC TCTTCCACCT 
TCATCACCCG CTGCTCCCAT TGCCACTTTT TCTTTGCTTC CTGCTGGTGG AGCCCCCACT 
GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG TCATCTGCTG CGGTCACTGG GGAGCCCCCT 
TCATATTCCA GTGGCTCCGA CAGCTCCAAA GCAGCCCCGG GCCCTGGCCC ATCAACCTTC 
TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA GCCCCCACCC CTGCAGCGTC TCCCGTGGCT 
CCATCAGCTG CTTCATTCTC GTTTGGATCA TCTGGTTTTA AGCCTACCCT GGAAAGCACA 
CCAGTGCCAA GTGTGTCTGC TCCAAATATA GCAGTGAAGC CCTCCTTCCC ACCCTCAACC 
TCTGCTGTCA AAGTCAACCT TAGTGAAAAG TTTACTGCCG CAGCTACCTC TACTCCTGTT 
AGTAGCTCCC AGAGCGCACC CTCGATGCCG CCATTCTCTT CTGCCTCCAA GCCAGCTACT 
TCTGGACCGC TCAGCCACCC CACGCCTCTC TCAGCATCAC CTAGTTCCGT GCCATTGAAG 
TCCTCAGTCT TGCCCTCACC ATCAGCAGGA CGATCTGCTC AGGGCAGTCC AAGCCCAGTG 
CCCTCAATGG TACAGAAATC ACCCAGGATA ACCCCTCCAG TGGCAAAGCC AGGCTCTCCC 
CAGGCAAAGT CACTTCAGCC TGCTGTTGCA GAGAAGCAGG GACATCAGTG GAAAGATTCA 
GATCCTGTAA TGGCTGGAAT TGGGGAGGAG ATTGCACACT TTCAGAAGGA GTTGGAAGAG 
TTAAAAGCCC GAACTTCCAA AGCCTGTTTC CAAGTGGGCA CTTCTGAGGA GATGAAGATG 
CTGCGAACAG AATCAGATGA CTTGCATACC TTTCTTTTGG AGATTAAAGA GACCACAGAG 
TCGCTTCATG GAGACATAAG TAGCCTGAAA ACAACTTTAC TTGAGGGCTT TGCTGGTGTT 
GAGGAAGCCA GAGAACAAAA TGAAAGAAAT CGTGACTCTG GTTATCTGCA TTTGCTTTAT 
AAAAGACCAC TGGATCCCAA GAGTGAAGCT CAGCTTCAGG AAATTCGGCG CCTTCATCAG 
TATGTGAAAT TTGCTGTCCA AGATGTGAAT GATGTTCTAG ATTTGGAGTG GGATCGGCAT 
CTGGAACAAA AGAAAAAACA AAGGCACCTG CTTGTGCCAG AGCGAGAGAC ACTGTTTAAC 
ACCCTAGCCA ACAACCGGGA AATCATCAAC CAACAGAGGA AGAGGCTGAA TCACCTGGTG 
GACAGTCTTC AGCAGCTCCG CCTTTACAAC CAGACTTCCC CGTGGAGCCT GCCCTCGGCT 
GTTCCTTCCC AGAGCAGCAT TCACAGTTTT GACAGTGACC TTGAAAGCCT GCGCAATGCT 
TTGTTGAAAA CCACCATAGA ATCTCACACC AAATCCTTGC CCAAAGTACC AGCCAAACTG 
TCCCCCATGA AACAGGCACA ACTGAGAAAC TTCTTGGCCA AGAGGAAGAC CCCACCAGTG 
AGATCCACTG CTCCAGCCAG CCTGTCTCGA TCAGCCTTTC TGTCTCAGAG ATATTATGAA 
GACTTGGATG AAGTCAGCTC AACGTCATCT GTCTCCCAGT CTCTGGAGAG TGAAGATGCA 
CGGACGTCCT ATAAAGATGA GGAGGCAGTG GTTCAGGCCC CTCGGCACGC CCCCGTGGTT 
CGCACTCCTT CCGTCCAGCC CAGTCTCTTG CCCCATGCAG CACCTTTTGC TAAATCTCAC 
CTGGTTCATG GTTCTTCACC TGGTGTGATG GGAACTTCAG TGGCTACATC TGCTAGCAAA 
ATTATTCCTC AAGGGGCCGA TAGCACAATG CTTGCCACGA AAACCGTGAA ACATGGTGCA 
CCTAGTCCTT CCCACCCCAT CTCAGCCCCG CAGGCAGCTG CCGCAGCAGC ACTCAGGCGG 
CAGATGGCCA GTCAGGCACC AGCTGTAAAC ACTTTGACTG AATCAACGTT GAAGAATGTC 
CCTCAAGTGG TAAATGTGCA GGAATTGAAG AATAACCCTG CAACCCCTTC TACAGCCATG 
GGTTCTTCAG TGGCCTACTC CGCAGCCAAA ACACCTCACC CAGTGTTGAC CCCAGTGGCC 
GCTAACCAAG CCAAGCAGGG GTCTCTAATA AATCCCCTTA AGCCATCTGG GCCTACACCA 
GCATCCGGTC AGTTATCATC TGGTGACAAA GCTTCAGGGA CAGCCAAGAT AGAAACAGCT 
GTGACTTCGA CCCCATCTGC TTCTGGGCAG TTCAGCAAGC CTTTCTCATT TTCTCCATCA 
GGGACTGGCT TTAATTTTGG GATAATCACA CCAACACCGT CTTCTAATTT CACTGCTGCA 
CAAGGGGCAA CACCCTCCAC TAAAGAGTCA AGCCAGCTGG ATGCATTCTC ATTTGGTGGG 
GGAAGCAAAC CTTCTTATGA GGCCATTCCT GAAAGCTCAC CTCCCTCAGG AATCACATCC 
GCATCAAACA CCACCCCAGG AGAACCTGCC ACATCTAGCA GCAGACCTGT GGCACCTTCT 
GGAACTGCTC TTTCCACTAC CTCTAGTAAG CTGGAAACCC CACCGTCCAA GCTGGGAGAG 
CTTCTGTTTC CAAGTTCTTT GGCTGGAGAG ACTCTGGGAA GTTTTTCAGG ACTGCGTGTT 
GGCCAAGCAG ATGATTCTAC AAAGCCAACC AATAAGGCTT CATCCACAAG CCTAACTAGT 
ACCCAGCCAA CCAAGACGTC AGGCGTGCCC TCAGGGTTTA ATTTTACTGC CCCCCCAGTG 
TTAGGGAAGC AAACGGAGCC CCCTGTGACC TCCTCTGCAA CCACCACCTC AGTAGCACCA 
CCAGCAGCCA CCAGCACTTC CTCAACTGGC GTTTTTGGCA GTCTGCCGGT CACCAGTGCA 
GGATCCTCTG GGGTCATCAG TTTTGGTGGG ACATCTCTAA GTGGTGGCAA GACTAGTTTT 
TCATTTGGAA GCCAACAGAC CAATAGCACA GTGCCCCCAT CTGCCCCACC GCCTACTACA 
GCTGCCACTC CCCTTCCAAC ATCATTCCCC ACATTGTCGT TCGGTAGCCT CCTGAGTTCA 
GCAACTACCC CCTCCCTGCC TATGTCCTCT GGCAGAAGCA CAGAAGAGGC CACTTCATCA 
GCTTTGCCTG AGAAGCCAGG TGACAGTGAG GTCTCAGCAT CAGCAGCCTC ACTTCTAGAG 
GAGCAACAGT CAGCCCAGCT TCCCCAGGCT CCTCCGCAAA CTTCTGACTC TGTTAAAAAA 
GAACCTGCTC TTGCCCAGCC TGCAGTCAGC AACTCTGGCA CTGCAGCATC TAGTACTAGT 
CTTGTAGCAC TTTCTGCAGA GGCTACCCCA ACCACCACCG GGGTCCCTGA TGCCAGGTCG 
GAGGCAGTAC CACCTGCTTC CTCCTTTTCT GTGCCTGGGC AGACTGCTGT CACAGCAGCT 
GCTATCTCAA GTGCAGGCCC TGTGGCCGTC GAAACATCAA GTGCCCCCAT AACCTCCAGC 
ACCACGTCCG TTGTTGCTCC CGGCCCATCT GCAGAGGCAG CAGCATTTGG TACCGTCACT 
TCTGGCTCAT CAGTCTTTGC TCAGCCCCCT GCTGCCAGTT CTAGCTCAGC TTTCAACCAG 
CTCACCAACA ACACAGCCAC TGCCACTTCT GCCACGCCCG TGTTTGGGCA AGTGGCAGCC 
AGCACTGCAC CAAGTCTGTT TGGGCAGCAG ACTGGTAGCA CAGCCAGCAC AGCAGCTGCC 
ACACCACAGG TCAGCAGCTC AGGGTTTAGC AGCCCAGCTT TTGGCACCAC AGCCCCAGGG 
GTCTTTGGAC AGACAACCTT CGGGCAGGCC TCAGTGTTTG GGCAGTCAGC GAGCAGTGCC 
GCAAGTGGCT TTTCCTTCAG TCAGCCTGGG TTCAGTTCCG TGCCTGCCTT CGGTCAGCCT 
GCTTCCTCCA CCCCCACATC CACCAGTGGA AGTGTCTTTG GTGCCGCCTC AAGTACCAGT 
AGCTCCAGTT CCTTCTCATT TGGACAGTCT TCTGCCAACA CAGGAGGGGG GCTGTTTGGC 
CAAAGCAACG CTCCTGCTTT TGGGCAGAGT CCTGGCTTTG GACAGGGAGG CTCTGTATTT 
GGTGGTACCT CAGCTGCCAC CACAACAGCA GTATCCTCTG GGTTCAGCTT TTGTCAAGCT 
TCAGGTTTTG GGTCTAGTAA TACTGGTTCT GTGTTTGGTC AAGCAGCCAG TACTGGTGGA 
ATAGTCTTTG GCCAGCAGTC ATCGTCTTCC AGTGGTAGCG TGTTTGGGTC TGGAAACACT 
GGAAGAGGGG GAGGTTTCTT CAGTGGCCTT GGAGGAAAAC CCAGTCAGGA TGCAGCCAAC 
AAAAACCCAT TCAGCTCGGC CAGTGGGGGC TTTGGATCCA CAGCTACCTC AAATACCTCT 
AACCTATTTG GAAACAGTGG GGCCAAGACA TTTGGTGGAT TTGCCAGCTC GTCGTTTGGA 
GAGCAGAAAC CCACTGGCAC TTTCAGCTCT GGAGGAGGAA GTGTGGCATC CCAAGGCTTT 
GGGTTTTCCT CTCCAAACAA AACAGGTGGC TTCGGTGCTG CTCCAGTGTT TGGCAGCCCT 
CCTACTTTTG GGGGATCCCC TGGGTTTGGA GGGGTGCCAG CATTCGGTTC AGCCCCAGCC 
TTTACAAGCC CTCTGGGCTC GACGGGAGGC AAAGTGTTCG GAGAGGGCAC TGCGGCTGCC 
AGCGCAGGAG GATTCGGGTT TGGGAGCAGC AGCAACACCA CATCCTTCGG CACGCTCGCG 
AGTCAGAATG CCCCCACTTT CGGATCACTG TCCCAACAGA CTTCTGGTTT TGGGACCCAG 
AGTAGCGGAT TCTCTGGTTT TGGATCAGGC ACAGGAGGGT TCAGCTTTGG ATCAAATAAC 
TCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( XM_024252125.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024107893.1 Pongo abelii nucleoporin 214 (NUP214), transcript variant X4, mRNA.

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CloneID	OSa186579
Clone ID Related Accession (Same CDS sequence)	XM_024252125.1
Accession Version	XM_024252125.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	nuclear pore complex protein Nup214 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036912.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTCGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTTGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAATAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGTGTGC ATGATGTCCG GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGGTTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCAATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTC ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGATTAA TTGGGAATCT 
TGGCTACTGG AGGATTCTAG TCGAGCTGAA TTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GAGTTGCCGT AGACTATACG AACCAAGTGG AAATCACCAT CAGTGATGAA 
AAGACTCTTC CTCCTGCTCC AGTTCTCATG TTACTTTCAA CAGATGGTGT GCTTTGTCCA 
TTTTATATGA TTAATCAAAA TCCTGGGGTT AAGTCTCTCA TCAAAACACC AGAGCGACTT 
TCATTAGAAG GAGAGCGACA GCCCAAGTCA CCAGGAAGTA CTCCCACTAC CCCGACCTCC 
TCTCAAGCCC CGCAGAAACT GGATGCTTCT GCAGCTGCAG CCCCTGCCTC TCTTCCACCT 
TCATCACCCG CTGCTCCCAT TGCCACTTTT TCTTTGCTTC CTGCTGGTGG AGCCCCCACT 
GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG TCATCTGCTG CGGTCACTGG GGAGCCCCCT 
TCATATTCCA GTGGCTCCGA CAGCTCCAAA GCAGCCCCGG GCCCTGGCCC ATCAACCTTC 
TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA GCCCCCACCC CTGCAGCGTC TCCCGTGGCT 
CCATCAGCTG CTTCATTCTC GTTTGGATCA TCTGGTTTTA AGCCTACCCT GGAAAGCACA 
CCAGTGCCAA GTGTGTCTGC TCCAAATATA GCAGTGAAGC CCTCCTTCCC ACCCTCAACC 
TCTGCTGTCA AAGTCAACCT TAGTGAAAAT AGCTCCCAGA GCGCACCCTC GATGCCGCCA 
TTCTCTTCTG CCTCCAAGCC AGCTACTTCT GGACCGCTCA GCCACCCCAC GCCTCTCTCA 
GCATCACCTA GTTCCGTGCC ATTGAAGTCC TCAGTCTTGC CCTCACCATC AGCAGGACGA 
TCTGCTCAGG GCAGTCCAAG CCCAGTGCCC TCAATGGTAC AGAAATCACC CAGGATAACC 
CCTCCAGTGG CAAAGCCAGG CTCTCCCCAG GCAAAGTCAC TTCAGCCTGC TGTTGCAGAG 
AAGCAGGGAC ATCAGTGGAA AGATTCAGAT CCTGTAATGG CTGGAATTGG GGAGGAGATT 
GCACACTTTC AGAAGGAGTT GGAAGAGTTA AAAGCCCGAA CTTCCAAAGC CTGTTTCCAA 
GTGGGCACTT CTGAGGAGAT GAAGATGCTG CGAACAGAAT CAGATGACTT GCATACCTTT 
CTTTTGGAGA TTAAAGAGAC CACAGAGTCG CTTCATGGAG ACATAAGTAG CCTGAAAACA 
ACTTTACTTG AGGGCTTTGC TGGTGTTGAG GAAGCCAGAG AACAAAATGA AAGAAATCGT 
GACTCTGGTT ATCTGCATTT GCTTTATAAA AGACCACTGG ATCCCAAGAG TGAAGCTCAG 
CTTCAGGAAA TTCGGCGCCT TCATCAGTAT GTGAAATTTG CTGTCCAAGA TGTGAATGAT 
GTTCTAGATT TGGAGTGGGA TCGGCATCTG GAACAAAAGA AAAAACAAAG GCACCTGCTT 
GTGCCAGAGC GAGAGACACT GTTTAACACC CTAGCCAACA ACCGGGAAAT CATCAACCAA 
CAGAGGAAGA GGCTGAATCA CCTGGTGGAC AGTCTTCAGC AGCTCCGCCT TTACAACCAG 
ACTTCCCCGT GGAGCCTGCC CTCGGCTGTT CCTTCCCAGA GCAGCATTCA CAGTTTTGAC 
AGTGACCTTG AAAGCCTGCG CAATGCTTTG TTGAAAACCA CCATAGAATC TCACACCAAA 
TCCTTGCCCA AAGTACCAGC CAAACTGTCC CCCATGAAAC AGGCACAACT GAGAAACTTC 
TTGGCCAAGA GGAAGACCCC ACCAGTGAGA TCCACTGCTC CAGCCAGCCT GTCTCGATCA 
GCCTTTCTGT CTCAGAGATA TTATGAAGAC TTGGATGAAG TCAGCTCAAC GTCATCTGTC 
TCCCAGTCTC TGGAGAGTGA AGATGCACGG ACGTCCTATA AAGATGAGGA GGCAGTGGTT 
CAGGCCCCTC GGCACGCCCC CGTGGTTCGC ACTCCTTCCG TCCAGCCCAG TCTCTTGCCC 
CATGCAGCAC CTTTTGCTAA ATCTCACCTG GTTCATGGTT CTTCACCTGG TGTGATGGGA 
ACTTCAGTGG CTACATCTGC TAGCAAAATT ATTCCTCAAG GGGCCGATAG CACAATGCTT 
GCCACGAAAA CCGTGAAACA TGGTGCACCT AGTCCTTCCC ACCCCATCTC AGCCCCGCAG 
GCAGCTGCCG CAGCAGCACT CAGGCGGCAG ATGGCCAGTC AGGCACCAGC TGTAAACACT 
TTGACTGAAT CAACGTTGAA GAATGTCCCT CAAGTGGTAA ATGTGCAGGA ATTGAAGAAT 
AACCCTGCAA CCCCTTCTAC AGCCATGGGT TCTTCAGTGG CCTACTCCGC AGCCAAAACA 
CCTCACCCAG TGTTGACCCC AGTGGCCGCT AACCAAGCCA AGCAGGGGTC TCTAATAAAT 
CCCCTTAAGC CATCTGGGCC TACACCAGCA TCCGGTCAGT TATCATCTGG TGACAAAGCT 
TCAGGGACAG CCAAGATAGA AACAGCTGTG ACTTCGACCC CATCTGCTTC TGGGCAGTTC 
AGCAAGCCTT TCTCATTTTC TCCATCAGGG ACTGGCTTTA ATTTTGGGAT AATCACACCA 
ACACCGTCTT CTAATTTCAC TGCTGCACAA GGGGCAACAC CCTCCACTAA AGAGTCAAGC 
CAGCTGGATG CATTCTCATT TGGTGGGGGA AGCAAACCTT CTTATGAGGC CATTCCTGAA 
AGCTCACCTC CCTCAGGAAT CACATCCGCA TCAAACACCA CCCCAGGAGA ACCTGCCACA 
TCTAGCAGCA GACCTGTGGC ACCTTCTGGA ACTGCTCTTT CCACTACCTC TAGTAAGCTG 
GAAACCCCAC CGTCCAAGCT GGGAGAGCTT CTGTTTCCAA GTTCTTTGGC TGGAGAGACT 
CTGGGAAGTT TTTCAGGACT GCGTGTTGGC CAAGCAGATG ATTCTACAAA GCCAACCAAT 
AAGGCTTCAT CCACAAGCCT AACTAGTACC CAGCCAACCA AGACGTCAGG CGTGCCCTCA 
GGGTTTAATT TTACTGCCCC CCCAGTGTTA GGGAAGCAAA CGGAGCCCCC TGTGACCTCC 
TCTGCAACCA CCACCTCAGT AGCACCACCA GCAGCCACCA GCACTTCCTC AACTGGCGTT 
TTTGGCAGTC TGCCGGTCAC CAGTGCAGGA TCCTCTGGGG TCATCAGTTT TGGTGGGACA 
TCTCTAAGTG GTGGCAAGAC TAGTTTTTCA TTTGGAAGCC AACAGACCAA TAGCACAGTG 
CCCCCATCTG CCCCACCGCC TACTACAGCT GCCACTCCCC TTCCAACATC ATTCCCCACA 
TTGTCGTTCG GTAGCCTCCT GAGTTCAGCA ACTACCCCCT CCCTGCCTAT GTCCTCTGGC 
AGAAGCACAG AAGAGGCCAC TTCATCAGCT TTGCCTGAGA AGCCAGGTGA CAGTGAGGTC 
TCAGCATCAG CAGCCTCACT TCTAGAGGAG CAACAGTCAG CCCAGCTTCC CCAGGCTCCT 
CCGCAAACTT CTGACTCTGT TAAAAAAGAA CCTGCTCTTG CCCAGCCTGC AGTCAGCAAC 
TCTGGCACTG CAGCATCTAG TACTAGTCTT GTAGCACTTT CTGCAGAGGC TACCCCAACC 
ACCACCGGGG TCCCTGATGC CAGGTCGGAG GCAGTACCAC CTGCTTCCTC CTTTTCTGTG 
CCTGGGCAGA CTGCTGTCAC AGCAGCTGCT ATCTCAAGTG CAGGCCCTGT GGCCGTCGAA 
ACATCAAGTG CCCCCATAAC CTCCAGCACC ACGTCCGTTG TTGCTCCCGG CCCATCTGCA 
GAGGCAGCAG CATTTGGTAC CGTCACTTCT GGCTCATCAG TCTTTGCTCA GCCCCCTGCT 
GCCAGTTCTA GCTCAGCTTT CAACCAGCTC ACCAACAACA CAGCCACTGC CACTTCTGCC 
ACGCCCGTGT TTGGGCAAGT GGCAGCCAGC ACTGCACCAA GTCTGTTTGG GCAGCAGACT 
GGTAGCACAG CCAGCACAGC AGCTGCCACA CCACAGGTCA GCAGCTCAGG GTTTAGCAGC 
CCAGCTTTTG GCACCACAGC CCCAGGGGTC TTTGGACAGA CAACCTTCGG GCAGGCCTCA 
GTGTTTGGGC AGTCAGCGAG CAGTGCCGCA AGTGGCTTTT CCTTCAGTCA GCCTGGGTTC 
AGTTCCGTGC CTGCCTTCGG TCAGCCTGCT TCCTCCACCC CCACATCCAC CAGTGGAAGT 
GTCTTTGGTG CCGCCTCAAG TACCAGTAGC TCCAGTTCCT TCTCATTTGG ACAGTCTTCT 
GCCAACACAG GAGGGGGGCT GTTTGGCCAA AGCAACGCTC CTGCTTTTGG GCAGAGTCCT 
GGCTTTGGAC AGGGAGGCTC TGTATTTGGT GGTACCTCAG CTGCCACCAC AACAGCAGTA 
TCCTCTGGGT TCAGCTTTTG TCAAGCTTCA GGTTTTGGGT CTAGTAATAC TGGTTCTGTG 
TTTGGTCAAG CAGCCAGTAC TGGTGGAATA GTCTTTGGCC AGCAGTCATC GTCTTCCAGT 
GGTAGCGTGT TTGGGTCTGG AAACACTGGA AGAGGGGGAG GTTTCTTCAG TGGCCTTGGA 
GGAAAACCCA GTCAGGATGC AGCCAACAAA AACCCATTCA GCTCGGCCAG TGGGGGCTTT 
GGATCCACAG CTACCTCAAA TACCTCTAAC CTATTTGGAA ACAGTGGGGC CAAGACATTT 
GGTGGATTTG CCAGCTCGTC GTTTGGAGAG CAGAAACCCA CTGGCACTTT CAGCTCTGGA 
GGAGGAAGTG TGGCATCCCA AGGCTTTGGG TTTTCCTCTC CAAACAAAAC AGGTGGCTTC 
GGTGCTGCTC CAGTGTTTGG CAGCCCTCCT ACTTTTGGGG GATCCCCTGG GTTTGGAGGG 
GTGCCAGCAT TCGGTTCAGC CCCAGCCTTT ACAAGCCCTC TGGGCTCGAC GGGAGGCAAA 
GTGTTCGGAG AGGGCACTGC GGCTGCCAGC GCAGGAGGAT TCGGGTTTGG GAGCAGCAGC 
AACACCACAT CCTTCGGCAC GCTCGCGAGT CAGAATGCCC CCACTTTCGG ATCACTGTCC 
CAACAGACTT CTGGTTTTGG GACCCAGAGT AGCGGATTCT CTGGTTTTGG ATCAGGCACA 
GGAGGGTTCA GCTTTGGATC AAATAACTCA TCTGTCCAGG GTTTTGGTGG CTGGCGAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024107893.1
CDS	139..6381
Translation	MGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSVCMMSGEYGSIIAFFDVRTFSNEAKQQKRPFAYHKLLKDAGGMVVDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQINWESWLLEDSSRAELPVTDKSDDSLPMGVAVDYTNQVEITISDEKTLPPAPVLMLLSTDGVLCPFYMINQNPGVKSLIKTPERLSLEGERQPKSPGSTPTTPTSSQAPQKLDASAAAAPASLPPSSPAAPIATFSLLPAGGAPTVFSFGSSSLKSSAAVTGEPPSYSSGSDSSKAAPGPGPSTFSFVPPSKASLAPTPAASPVAPSAASFSFGSSGFKPTLESTPVPSVSAPNIAVKPSFPPSTSAVKVNLSENSSQSAPSMPPFSSASKPATSGPLSHPTPLSASPSSVPLKSSVLPSPSAGRSAQGSPSPVPSMVQKSPRITPPVAKPGSPQAKSLQPAVAEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKARTSKACFQVGTSEEMKMLRTESDDLHTFLLEIKETTESLHGDISSLKTTLLEGFAGVEEAREQNERNRDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDRHLEQKKKQRHLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYNQTSPWSLPSAVPSQSSIHSFDSDLESLRNALLKTTIESHTKSLPKVPAKLSPMKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEVSSTSSVSQSLESEDARTSYKDEEAVVQAPRHAPVVRTPSVQPSLLPHAAPFAKSHLVHGSSPGVMGTSVATSASKIIPQGADSTMLATKTVKHGAPSPSHPISAPQAAAAAALRRQMASQAPAVNTLTESTLKNVPQVVNVQELKNNPATPSTAMGSSVAYSAAKTPHPVLTPVAANQAKQGSLINPLKPSGPTPASGQLSSGDKASGTAKIETAVTSTPSASGQFSKPFSFSPSGTGFNFGIITPTPSSNFTAAQGATPSTKESSQLDAFSFGGGSKPSYEAIPESSPPSGITSASNTTPGEPATSSSRPVAPSGTALSTTSSKLETPPSKLGELLFPSSLAGETLGSFSGLRVGQADDSTKPTNKASSTSLTSTQPTKTSGVPSGFNFTAPPVLGKQTEPPVTSSATTTSVAPPAATSTSSTGVFGSLPVTSAGSSGVISFGGTSLSGGKTSFSFGSQQTNSTVPPSAPPPTTAATPLPTSFPTLSFGSLLSSATTPSLPMSSGRSTEEATSSALPEKPGDSEVSASAASLLEEQQSAQLPQAPPQTSDSVKKEPALAQPAVSNSGTAASSTSLVALSAEATPTTTGVPDARSEAVPPASSFSVPGQTAVTAAAISSAGPVAVETSSAPITSSTTSVVAPGPSAEAAAFGTVTSGSSVFAQPPAASSSSAFNQLTNNTATATSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGFSSPAFGTTAPGVFGQTTFGQASVFGQSASSAASGFSFSQPGFSSVPAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSANTGGGLFGQSNAPAFGQSPGFGQGGSVFGGTSAATTTAVSSGFSFCQASGFGSSNTGSVFGQAASTGGIVFGQQSSSSSGSVFGSGNTGRGGGFFSGLGGKPSQDAANKNPFSSASGGFGSTATSNTSNLFGNSGAKTFGGFASSSFGEQKPTGTFSSGGGSVASQGFGFSSPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSNTTSFGTLASQNAPTFGSLSQQTSGFGTQSSGFSGFGSGTGGFSFGSNNSSVQGFGGWRS

Target ORF information:

RefSeq Version	XM_024252125.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii nucleoporin 214 (NUP214), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024252125.1

ORF Insert Sequence:

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTCGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTTGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAATAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGTGTGC ATGATGTCCG GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGGTTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCAATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTC ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGATTAA TTGGGAATCT 
TGGCTACTGG AGGATTCTAG TCGAGCTGAA TTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GAGTTGCCGT AGACTATACG AACCAAGTGG AAATCACCAT CAGTGATGAA 
AAGACTCTTC CTCCTGCTCC AGTTCTCATG TTACTTTCAA CAGATGGTGT GCTTTGTCCA 
TTTTATATGA TTAATCAAAA TCCTGGGGTT AAGTCTCTCA TCAAAACACC AGAGCGACTT 
TCATTAGAAG GAGAGCGACA GCCCAAGTCA CCAGGAAGTA CTCCCACTAC CCCGACCTCC 
TCTCAAGCCC CGCAGAAACT GGATGCTTCT GCAGCTGCAG CCCCTGCCTC TCTTCCACCT 
TCATCACCCG CTGCTCCCAT TGCCACTTTT TCTTTGCTTC CTGCTGGTGG AGCCCCCACT 
GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG TCATCTGCTG CGGTCACTGG GGAGCCCCCT 
TCATATTCCA GTGGCTCCGA CAGCTCCAAA GCAGCCCCGG GCCCTGGCCC ATCAACCTTC 
TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA GCCCCCACCC CTGCAGCGTC TCCCGTGGCT 
CCATCAGCTG CTTCATTCTC GTTTGGATCA TCTGGTTTTA AGCCTACCCT GGAAAGCACA 
CCAGTGCCAA GTGTGTCTGC TCCAAATATA GCAGTGAAGC CCTCCTTCCC ACCCTCAACC 
TCTGCTGTCA AAGTCAACCT TAGTGAAAAT AGCTCCCAGA GCGCACCCTC GATGCCGCCA 
TTCTCTTCTG CCTCCAAGCC AGCTACTTCT GGACCGCTCA GCCACCCCAC GCCTCTCTCA 
GCATCACCTA GTTCCGTGCC ATTGAAGTCC TCAGTCTTGC CCTCACCATC AGCAGGACGA 
TCTGCTCAGG GCAGTCCAAG CCCAGTGCCC TCAATGGTAC AGAAATCACC CAGGATAACC 
CCTCCAGTGG CAAAGCCAGG CTCTCCCCAG GCAAAGTCAC TTCAGCCTGC TGTTGCAGAG 
AAGCAGGGAC ATCAGTGGAA AGATTCAGAT CCTGTAATGG CTGGAATTGG GGAGGAGATT 
GCACACTTTC AGAAGGAGTT GGAAGAGTTA AAAGCCCGAA CTTCCAAAGC CTGTTTCCAA 
GTGGGCACTT CTGAGGAGAT GAAGATGCTG CGAACAGAAT CAGATGACTT GCATACCTTT 
CTTTTGGAGA TTAAAGAGAC CACAGAGTCG CTTCATGGAG ACATAAGTAG CCTGAAAACA 
ACTTTACTTG AGGGCTTTGC TGGTGTTGAG GAAGCCAGAG AACAAAATGA AAGAAATCGT 
GACTCTGGTT ATCTGCATTT GCTTTATAAA AGACCACTGG ATCCCAAGAG TGAAGCTCAG 
CTTCAGGAAA TTCGGCGCCT TCATCAGTAT GTGAAATTTG CTGTCCAAGA TGTGAATGAT 
GTTCTAGATT TGGAGTGGGA TCGGCATCTG GAACAAAAGA AAAAACAAAG GCACCTGCTT 
GTGCCAGAGC GAGAGACACT GTTTAACACC CTAGCCAACA ACCGGGAAAT CATCAACCAA 
CAGAGGAAGA GGCTGAATCA CCTGGTGGAC AGTCTTCAGC AGCTCCGCCT TTACAACCAG 
ACTTCCCCGT GGAGCCTGCC CTCGGCTGTT CCTTCCCAGA GCAGCATTCA CAGTTTTGAC 
AGTGACCTTG AAAGCCTGCG CAATGCTTTG TTGAAAACCA CCATAGAATC TCACACCAAA 
TCCTTGCCCA AAGTACCAGC CAAACTGTCC CCCATGAAAC AGGCACAACT GAGAAACTTC 
TTGGCCAAGA GGAAGACCCC ACCAGTGAGA TCCACTGCTC CAGCCAGCCT GTCTCGATCA 
GCCTTTCTGT CTCAGAGATA TTATGAAGAC TTGGATGAAG TCAGCTCAAC GTCATCTGTC 
TCCCAGTCTC TGGAGAGTGA AGATGCACGG ACGTCCTATA AAGATGAGGA GGCAGTGGTT 
CAGGCCCCTC GGCACGCCCC CGTGGTTCGC ACTCCTTCCG TCCAGCCCAG TCTCTTGCCC 
CATGCAGCAC CTTTTGCTAA ATCTCACCTG GTTCATGGTT CTTCACCTGG TGTGATGGGA 
ACTTCAGTGG CTACATCTGC TAGCAAAATT ATTCCTCAAG GGGCCGATAG CACAATGCTT 
GCCACGAAAA CCGTGAAACA TGGTGCACCT AGTCCTTCCC ACCCCATCTC AGCCCCGCAG 
GCAGCTGCCG CAGCAGCACT CAGGCGGCAG ATGGCCAGTC AGGCACCAGC TGTAAACACT 
TTGACTGAAT CAACGTTGAA GAATGTCCCT CAAGTGGTAA ATGTGCAGGA ATTGAAGAAT 
AACCCTGCAA CCCCTTCTAC AGCCATGGGT TCTTCAGTGG CCTACTCCGC AGCCAAAACA 
CCTCACCCAG TGTTGACCCC AGTGGCCGCT AACCAAGCCA AGCAGGGGTC TCTAATAAAT 
CCCCTTAAGC CATCTGGGCC TACACCAGCA TCCGGTCAGT TATCATCTGG TGACAAAGCT 
TCAGGGACAG CCAAGATAGA AACAGCTGTG ACTTCGACCC CATCTGCTTC TGGGCAGTTC 
AGCAAGCCTT TCTCATTTTC TCCATCAGGG ACTGGCTTTA ATTTTGGGAT AATCACACCA 
ACACCGTCTT CTAATTTCAC TGCTGCACAA GGGGCAACAC CCTCCACTAA AGAGTCAAGC 
CAGCTGGATG CATTCTCATT TGGTGGGGGA AGCAAACCTT CTTATGAGGC CATTCCTGAA 
AGCTCACCTC CCTCAGGAAT CACATCCGCA TCAAACACCA CCCCAGGAGA ACCTGCCACA 
TCTAGCAGCA GACCTGTGGC ACCTTCTGGA ACTGCTCTTT CCACTACCTC TAGTAAGCTG 
GAAACCCCAC CGTCCAAGCT GGGAGAGCTT CTGTTTCCAA GTTCTTTGGC TGGAGAGACT 
CTGGGAAGTT TTTCAGGACT GCGTGTTGGC CAAGCAGATG ATTCTACAAA GCCAACCAAT 
AAGGCTTCAT CCACAAGCCT AACTAGTACC CAGCCAACCA AGACGTCAGG CGTGCCCTCA 
GGGTTTAATT TTACTGCCCC CCCAGTGTTA GGGAAGCAAA CGGAGCCCCC TGTGACCTCC 
TCTGCAACCA CCACCTCAGT AGCACCACCA GCAGCCACCA GCACTTCCTC AACTGGCGTT 
TTTGGCAGTC TGCCGGTCAC CAGTGCAGGA TCCTCTGGGG TCATCAGTTT TGGTGGGACA 
TCTCTAAGTG GTGGCAAGAC TAGTTTTTCA TTTGGAAGCC AACAGACCAA TAGCACAGTG 
CCCCCATCTG CCCCACCGCC TACTACAGCT GCCACTCCCC TTCCAACATC ATTCCCCACA 
TTGTCGTTCG GTAGCCTCCT GAGTTCAGCA ACTACCCCCT CCCTGCCTAT GTCCTCTGGC 
AGAAGCACAG AAGAGGCCAC TTCATCAGCT TTGCCTGAGA AGCCAGGTGA CAGTGAGGTC 
TCAGCATCAG CAGCCTCACT TCTAGAGGAG CAACAGTCAG CCCAGCTTCC CCAGGCTCCT 
CCGCAAACTT CTGACTCTGT TAAAAAAGAA CCTGCTCTTG CCCAGCCTGC AGTCAGCAAC 
TCTGGCACTG CAGCATCTAG TACTAGTCTT GTAGCACTTT CTGCAGAGGC TACCCCAACC 
ACCACCGGGG TCCCTGATGC CAGGTCGGAG GCAGTACCAC CTGCTTCCTC CTTTTCTGTG 
CCTGGGCAGA CTGCTGTCAC AGCAGCTGCT ATCTCAAGTG CAGGCCCTGT GGCCGTCGAA 
ACATCAAGTG CCCCCATAAC CTCCAGCACC ACGTCCGTTG TTGCTCCCGG CCCATCTGCA 
GAGGCAGCAG CATTTGGTAC CGTCACTTCT GGCTCATCAG TCTTTGCTCA GCCCCCTGCT 
GCCAGTTCTA GCTCAGCTTT CAACCAGCTC ACCAACAACA CAGCCACTGC CACTTCTGCC 
ACGCCCGTGT TTGGGCAAGT GGCAGCCAGC ACTGCACCAA GTCTGTTTGG GCAGCAGACT 
GGTAGCACAG CCAGCACAGC AGCTGCCACA CCACAGGTCA GCAGCTCAGG GTTTAGCAGC 
CCAGCTTTTG GCACCACAGC CCCAGGGGTC TTTGGACAGA CAACCTTCGG GCAGGCCTCA 
GTGTTTGGGC AGTCAGCGAG CAGTGCCGCA AGTGGCTTTT CCTTCAGTCA GCCTGGGTTC 
AGTTCCGTGC CTGCCTTCGG TCAGCCTGCT TCCTCCACCC CCACATCCAC CAGTGGAAGT 
GTCTTTGGTG CCGCCTCAAG TACCAGTAGC TCCAGTTCCT TCTCATTTGG ACAGTCTTCT 
GCCAACACAG GAGGGGGGCT GTTTGGCCAA AGCAACGCTC CTGCTTTTGG GCAGAGTCCT 
GGCTTTGGAC AGGGAGGCTC TGTATTTGGT GGTACCTCAG CTGCCACCAC AACAGCAGTA 
TCCTCTGGGT TCAGCTTTTG TCAAGCTTCA GGTTTTGGGT CTAGTAATAC TGGTTCTGTG 
TTTGGTCAAG CAGCCAGTAC TGGTGGAATA GTCTTTGGCC AGCAGTCATC GTCTTCCAGT 
GGTAGCGTGT TTGGGTCTGG AAACACTGGA AGAGGGGGAG GTTTCTTCAG TGGCCTTGGA 
GGAAAACCCA GTCAGGATGC AGCCAACAAA AACCCATTCA GCTCGGCCAG TGGGGGCTTT 
GGATCCACAG CTACCTCAAA TACCTCTAAC CTATTTGGAA ACAGTGGGGC CAAGACATTT 
GGTGGATTTG CCAGCTCGTC GTTTGGAGAG CAGAAACCCA CTGGCACTTT CAGCTCTGGA 
GGAGGAAGTG TGGCATCCCA AGGCTTTGGG TTTTCCTCTC CAAACAAAAC AGGTGGCTTC 
GGTGCTGCTC CAGTGTTTGG CAGCCCTCCT ACTTTTGGGG GATCCCCTGG GTTTGGAGGG 
GTGCCAGCAT TCGGTTCAGC CCCAGCCTTT ACAAGCCCTC TGGGCTCGAC GGGAGGCAAA 
GTGTTCGGAG AGGGCACTGC GGCTGCCAGC GCAGGAGGAT TCGGGTTTGG GAGCAGCAGC 
AACACCACAT CCTTCGGCAC GCTCGCGAGT CAGAATGCCC CCACTTTCGG ATCACTGTCC 
CAACAGACTT CTGGTTTTGG GACCCAGAGT AGCGGATTCT CTGGTTTTGG ATCAGGCACA 
GGAGGGTTCA GCTTTGGATC AAATAACTCA TCTGTCCAGG GTTTTGGTGG CTGGCGAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( XM_024252126.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024107894.1 Pongo abelii nucleoporin 214 (NUP214), transcript variant X5, mRNA.

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CloneID	OSa186580
Clone ID Related Accession (Same CDS sequence)	XM_024252126.1
Accession Version	XM_024252126.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	nuclear pore complex protein Nup214 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036912.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTCGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTTGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAATAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGTGTGC ATGATGTCCG GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGGTTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCAATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTC ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGATTAA TTGGGAATCT 
TGGCTACTGG AGGATTCTAG TCGAGCTGAA TTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GAGTTGCCGT AGACTATACG AACCAAGTGG AAATCACCAT CAGTGATGAA 
AAGACTCTTC CTCCTGCTCC AGTTCTCATG TTACTTTCAA CAGATGGTGT GCTTTGTCCA 
TTTTATATGA TTAATCAAAA TCCTGGGGTT AAGTCTCTCA TCAAAACACC AGAGCGACTT 
TCATTAGAAG GAGAGCGACA GCCCAAGTCA CCAGGAAGTA CTCCCACTAC CCCGACCTCC 
TCTCAAGCCC CGCAGAAACT GGATGCTTCT GCAGCTGCAG CCCCTGCCTC TCTTCCACCT 
TCATCACCCG CTGCTCCCAT TGCCACTTTT TCTTTGCTTC CTGCTGGTGG AGCCCCCACT 
GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG TCATCTGCTG CGGTCACTGG GGAGCCCCCT 
TCATATTCCA GTGGCTCCGA CAGCTCCAAA GCAGCCCCGG GCCCTGGCCC ATCAACCTTC 
TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA GCCCCCACCC CTGCAGCGTC TCCCGTGGCT 
CCATCAGCTG CTTCATTCTC GTTTGGATCA TCTGGTTTTA AGCCTACCCT GGAAAGCACA 
CCAGTGCCAA GTGTGTCTGC TCCAAATATA GCAGTGAAGC CCTCCTTCCC ACCCTCAACC 
TCTGCTGTCA AAGTCAACCT TAGTGAAAAT AGCTCCCAGA GCGCACCCTC GATGCCGCCA 
TTCTCTTCTG CCTCCAAGCC AGCTACTTCT GGACCGCTCA GCCACCCCAC GCCTCTCTCA 
GCATCACCTA GTTCCGTGCC ATTGAAGTCC TCAGTCTTGC CCTCACCATC AGGACGATCT 
GCTCAGGGCA GTCCAAGCCC AGTGCCCTCA ATGGTACAGA AATCACCCAG GATAACCCCT 
CCAGTGGCAA AGCCAGGCTC TCCCCAGGCA AAGTCACTTC AGCCTGCTGT TGCAGAGAAG 
CAGGGACATC AGTGGAAAGA TTCAGATCCT GTAATGGCTG GAATTGGGGA GGAGATTGCA 
CACTTTCAGA AGGAGTTGGA AGAGTTAAAA GCCCGAACTT CCAAAGCCTG TTTCCAAGTG 
GGCACTTCTG AGGAGATGAA GATGCTGCGA ACAGAATCAG ATGACTTGCA TACCTTTCTT 
TTGGAGATTA AAGAGACCAC AGAGTCGCTT CATGGAGACA TAAGTAGCCT GAAAACAACT 
TTACTTGAGG GCTTTGCTGG TGTTGAGGAA GCCAGAGAAC AAAATGAAAG AAATCGTGAC 
TCTGGTTATC TGCATTTGCT TTATAAAAGA CCACTGGATC CCAAGAGTGA AGCTCAGCTT 
CAGGAAATTC GGCGCCTTCA TCAGTATGTG AAATTTGCTG TCCAAGATGT GAATGATGTT 
CTAGATTTGG AGTGGGATCG GCATCTGGAA CAAAAGAAAA AACAAAGGCA CCTGCTTGTG 
CCAGAGCGAG AGACACTGTT TAACACCCTA GCCAACAACC GGGAAATCAT CAACCAACAG 
AGGAAGAGGC TGAATCACCT GGTGGACAGT CTTCAGCAGC TCCGCCTTTA CAACCAGACT 
TCCCCGTGGA GCCTGCCCTC GGCTGTTCCT TCCCAGAGCA GCATTCACAG TTTTGACAGT 
GACCTTGAAA GCCTGCGCAA TGCTTTGTTG AAAACCACCA TAGAATCTCA CACCAAATCC 
TTGCCCAAAG TACCAGCCAA ACTGTCCCCC ATGAAACAGG CACAACTGAG AAACTTCTTG 
GCCAAGAGGA AGACCCCACC AGTGAGATCC ACTGCTCCAG CCAGCCTGTC TCGATCAGCC 
TTTCTGTCTC AGAGATATTA TGAAGACTTG GATGAAGTCA GCTCAACGTC ATCTGTCTCC 
CAGTCTCTGG AGAGTGAAGA TGCACGGACG TCCTATAAAG ATGAGGAGGC AGTGGTTCAG 
GCCCCTCGGC ACGCCCCCGT GGTTCGCACT CCTTCCGTCC AGCCCAGTCT CTTGCCCCAT 
GCAGCACCTT TTGCTAAATC TCACCTGGTT CATGGTTCTT CACCTGGTGT GATGGGAACT 
TCAGTGGCTA CATCTGCTAG CAAAATTATT CCTCAAGGGG CCGATAGCAC AATGCTTGCC 
ACGAAAACCG TGAAACATGG TGCACCTAGT CCTTCCCACC CCATCTCAGC CCCGCAGGCA 
GCTGCCGCAG CAGCACTCAG GCGGCAGATG GCCAGTCAGG CACCAGCTGT AAACACTTTG 
ACTGAATCAA CGTTGAAGAA TGTCCCTCAA GTGGTAAATG TGCAGGAATT GAAGAATAAC 
CCTGCAACCC CTTCTACAGC CATGGGTTCT TCAGTGGCCT ACTCCGCAGC CAAAACACCT 
CACCCAGTGT TGACCCCAGT GGCCGCTAAC CAAGCCAAGC AGGGGTCTCT AATAAATCCC 
CTTAAGCCAT CTGGGCCTAC ACCAGCATCC GGTCAGTTAT CATCTGGTGA CAAAGCTTCA 
GGGACAGCCA AGATAGAAAC AGCTGTGACT TCGACCCCAT CTGCTTCTGG GCAGTTCAGC 
AAGCCTTTCT CATTTTCTCC ATCAGGGACT GGCTTTAATT TTGGGATAAT CACACCAACA 
CCGTCTTCTA ATTTCACTGC TGCACAAGGG GCAACACCCT CCACTAAAGA GTCAAGCCAG 
CTGGATGCAT TCTCATTTGG TGGGGGAAGC AAACCTTCTT ATGAGGCCAT TCCTGAAAGC 
TCACCTCCCT CAGGAATCAC ATCCGCATCA AACACCACCC CAGGAGAACC TGCCACATCT 
AGCAGCAGAC CTGTGGCACC TTCTGGAACT GCTCTTTCCA CTACCTCTAG TAAGCTGGAA 
ACCCCACCGT CCAAGCTGGG AGAGCTTCTG TTTCCAAGTT CTTTGGCTGG AGAGACTCTG 
GGAAGTTTTT CAGGACTGCG TGTTGGCCAA GCAGATGATT CTACAAAGCC AACCAATAAG 
GCTTCATCCA CAAGCCTAAC TAGTACCCAG CCAACCAAGA CGTCAGGCGT GCCCTCAGGG 
TTTAATTTTA CTGCCCCCCC AGTGTTAGGG AAGCAAACGG AGCCCCCTGT GACCTCCTCT 
GCAACCACCA CCTCAGTAGC ACCACCAGCA GCCACCAGCA CTTCCTCAAC TGGCGTTTTT 
GGCAGTCTGC CGGTCACCAG TGCAGGATCC TCTGGGGTCA TCAGTTTTGG TGGGACATCT 
CTAAGTGGTG GCAAGACTAG TTTTTCATTT GGAAGCCAAC AGACCAATAG CACAGTGCCC 
CCATCTGCCC CACCGCCTAC TACAGCTGCC ACTCCCCTTC CAACATCATT CCCCACATTG 
TCGTTCGGTA GCCTCCTGAG TTCAGCAACT ACCCCCTCCC TGCCTATGTC CTCTGGCAGA 
AGCACAGAAG AGGCCACTTC ATCAGCTTTG CCTGAGAAGC CAGGTGACAG TGAGGTCTCA 
GCATCAGCAG CCTCACTTCT AGAGGAGCAA CAGTCAGCCC AGCTTCCCCA GGCTCCTCCG 
CAAACTTCTG ACTCTGTTAA AAAAGAACCT GCTCTTGCCC AGCCTGCAGT CAGCAACTCT 
GGCACTGCAG CATCTAGTAC TAGTCTTGTA GCACTTTCTG CAGAGGCTAC CCCAACCACC 
ACCGGGGTCC CTGATGCCAG GTCGGAGGCA GTACCACCTG CTTCCTCCTT TTCTGTGCCT 
GGGCAGACTG CTGTCACAGC AGCTGCTATC TCAAGTGCAG GCCCTGTGGC CGTCGAAACA 
TCAAGTGCCC CCATAACCTC CAGCACCACG TCCGTTGTTG CTCCCGGCCC ATCTGCAGAG 
GCAGCAGCAT TTGGTACCGT CACTTCTGGC TCATCAGTCT TTGCTCAGCC CCCTGCTGCC 
AGTTCTAGCT CAGCTTTCAA CCAGCTCACC AACAACACAG CCACTGCCAC TTCTGCCACG 
CCCGTGTTTG GGCAAGTGGC AGCCAGCACT GCACCAAGTC TGTTTGGGCA GCAGACTGGT 
AGCACAGCCA GCACAGCAGC TGCCACACCA CAGGTCAGCA GCTCAGGGTT TAGCAGCCCA 
GCTTTTGGCA CCACAGCCCC AGGGGTCTTT GGACAGACAA CCTTCGGGCA GGCCTCAGTG 
TTTGGGCAGT CAGCGAGCAG TGCCGCAAGT GGCTTTTCCT TCAGTCAGCC TGGGTTCAGT 
TCCGTGCCTG CCTTCGGTCA GCCTGCTTCC TCCACCCCCA CATCCACCAG TGGAAGTGTC 
TTTGGTGCCG CCTCAAGTAC CAGTAGCTCC AGTTCCTTCT CATTTGGACA GTCTTCTGCC 
AACACAGGAG GGGGGCTGTT TGGCCAAAGC AACGCTCCTG CTTTTGGGCA GAGTCCTGGC 
TTTGGACAGG GAGGCTCTGT ATTTGGTGGT ACCTCAGCTG CCACCACAAC AGCAGTATCC 
TCTGGGTTCA GCTTTTGTCA AGCTTCAGGT TTTGGGTCTA GTAATACTGG TTCTGTGTTT 
GGTCAAGCAG CCAGTACTGG TGGAATAGTC TTTGGCCAGC AGTCATCGTC TTCCAGTGGT 
AGCGTGTTTG GGTCTGGAAA CACTGGAAGA GGGGGAGGTT TCTTCAGTGG CCTTGGAGGA 
AAACCCAGTC AGGATGCAGC CAACAAAAAC CCATTCAGCT CGGCCAGTGG GGGCTTTGGA 
TCCACAGCTA CCTCAAATAC CTCTAACCTA TTTGGAAACA GTGGGGCCAA GACATTTGGT 
GGATTTGCCA GCTCGTCGTT TGGAGAGCAG AAACCCACTG GCACTTTCAG CTCTGGAGGA 
GGAAGTGTGG CATCCCAAGG CTTTGGGTTT TCCTCTCCAA ACAAAACAGG TGGCTTCGGT 
GCTGCTCCAG TGTTTGGCAG CCCTCCTACT TTTGGGGGAT CCCCTGGGTT TGGAGGGGTG 
CCAGCATTCG GTTCAGCCCC AGCCTTTACA AGCCCTCTGG GCTCGACGGG AGGCAAAGTG 
TTCGGAGAGG GCACTGCGGC TGCCAGCGCA GGAGGATTCG GGTTTGGGAG CAGCAGCAAC 
ACCACATCCT TCGGCACGCT CGCGAGTCAG AATGCCCCCA CTTTCGGATC ACTGTCCCAA 
CAGACTTCTG GTTTTGGGAC CCAGAGTAGC GGATTCTCTG GTTTTGGATC AGGCACAGGA 
GGGTTCAGCT TTGGATCAAA TAACTCATCT GTCCAGGGTT TTGGTGGCTG GCGAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024107894.1
CDS	139..6378
Translation	MGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSVCMMSGEYGSIIAFFDVRTFSNEAKQQKRPFAYHKLLKDAGGMVVDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQINWESWLLEDSSRAELPVTDKSDDSLPMGVAVDYTNQVEITISDEKTLPPAPVLMLLSTDGVLCPFYMINQNPGVKSLIKTPERLSLEGERQPKSPGSTPTTPTSSQAPQKLDASAAAAPASLPPSSPAAPIATFSLLPAGGAPTVFSFGSSSLKSSAAVTGEPPSYSSGSDSSKAAPGPGPSTFSFVPPSKASLAPTPAASPVAPSAASFSFGSSGFKPTLESTPVPSVSAPNIAVKPSFPPSTSAVKVNLSENSSQSAPSMPPFSSASKPATSGPLSHPTPLSASPSSVPLKSSVLPSPSGRSAQGSPSPVPSMVQKSPRITPPVAKPGSPQAKSLQPAVAEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKARTSKACFQVGTSEEMKMLRTESDDLHTFLLEIKETTESLHGDISSLKTTLLEGFAGVEEAREQNERNRDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDRHLEQKKKQRHLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYNQTSPWSLPSAVPSQSSIHSFDSDLESLRNALLKTTIESHTKSLPKVPAKLSPMKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEVSSTSSVSQSLESEDARTSYKDEEAVVQAPRHAPVVRTPSVQPSLLPHAAPFAKSHLVHGSSPGVMGTSVATSASKIIPQGADSTMLATKTVKHGAPSPSHPISAPQAAAAAALRRQMASQAPAVNTLTESTLKNVPQVVNVQELKNNPATPSTAMGSSVAYSAAKTPHPVLTPVAANQAKQGSLINPLKPSGPTPASGQLSSGDKASGTAKIETAVTSTPSASGQFSKPFSFSPSGTGFNFGIITPTPSSNFTAAQGATPSTKESSQLDAFSFGGGSKPSYEAIPESSPPSGITSASNTTPGEPATSSSRPVAPSGTALSTTSSKLETPPSKLGELLFPSSLAGETLGSFSGLRVGQADDSTKPTNKASSTSLTSTQPTKTSGVPSGFNFTAPPVLGKQTEPPVTSSATTTSVAPPAATSTSSTGVFGSLPVTSAGSSGVISFGGTSLSGGKTSFSFGSQQTNSTVPPSAPPPTTAATPLPTSFPTLSFGSLLSSATTPSLPMSSGRSTEEATSSALPEKPGDSEVSASAASLLEEQQSAQLPQAPPQTSDSVKKEPALAQPAVSNSGTAASSTSLVALSAEATPTTTGVPDARSEAVPPASSFSVPGQTAVTAAAISSAGPVAVETSSAPITSSTTSVVAPGPSAEAAAFGTVTSGSSVFAQPPAASSSSAFNQLTNNTATATSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGFSSPAFGTTAPGVFGQTTFGQASVFGQSASSAASGFSFSQPGFSSVPAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSANTGGGLFGQSNAPAFGQSPGFGQGGSVFGGTSAATTTAVSSGFSFCQASGFGSSNTGSVFGQAASTGGIVFGQQSSSSSGSVFGSGNTGRGGGFFSGLGGKPSQDAANKNPFSSASGGFGSTATSNTSNLFGNSGAKTFGGFASSSFGEQKPTGTFSSGGGSVASQGFGFSSPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSNTTSFGTLASQNAPTFGSLSQQTSGFGTQSSGFSGFGSGTGGFSFGSNNSSVQGFGGWRS

Target ORF information:

RefSeq Version	XM_024252126.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii nucleoporin 214 (NUP214), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024252126.1

ORF Insert Sequence:

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTCGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTTGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAATAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGTGTGC ATGATGTCCG GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGGTTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCAATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTC ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGATTAA TTGGGAATCT 
TGGCTACTGG AGGATTCTAG TCGAGCTGAA TTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GAGTTGCCGT AGACTATACG AACCAAGTGG AAATCACCAT CAGTGATGAA 
AAGACTCTTC CTCCTGCTCC AGTTCTCATG TTACTTTCAA CAGATGGTGT GCTTTGTCCA 
TTTTATATGA TTAATCAAAA TCCTGGGGTT AAGTCTCTCA TCAAAACACC AGAGCGACTT 
TCATTAGAAG GAGAGCGACA GCCCAAGTCA CCAGGAAGTA CTCCCACTAC CCCGACCTCC 
TCTCAAGCCC CGCAGAAACT GGATGCTTCT GCAGCTGCAG CCCCTGCCTC TCTTCCACCT 
TCATCACCCG CTGCTCCCAT TGCCACTTTT TCTTTGCTTC CTGCTGGTGG AGCCCCCACT 
GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG TCATCTGCTG CGGTCACTGG GGAGCCCCCT 
TCATATTCCA GTGGCTCCGA CAGCTCCAAA GCAGCCCCGG GCCCTGGCCC ATCAACCTTC 
TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA GCCCCCACCC CTGCAGCGTC TCCCGTGGCT 
CCATCAGCTG CTTCATTCTC GTTTGGATCA TCTGGTTTTA AGCCTACCCT GGAAAGCACA 
CCAGTGCCAA GTGTGTCTGC TCCAAATATA GCAGTGAAGC CCTCCTTCCC ACCCTCAACC 
TCTGCTGTCA AAGTCAACCT TAGTGAAAAT AGCTCCCAGA GCGCACCCTC GATGCCGCCA 
TTCTCTTCTG CCTCCAAGCC AGCTACTTCT GGACCGCTCA GCCACCCCAC GCCTCTCTCA 
GCATCACCTA GTTCCGTGCC ATTGAAGTCC TCAGTCTTGC CCTCACCATC AGGACGATCT 
GCTCAGGGCA GTCCAAGCCC AGTGCCCTCA ATGGTACAGA AATCACCCAG GATAACCCCT 
CCAGTGGCAA AGCCAGGCTC TCCCCAGGCA AAGTCACTTC AGCCTGCTGT TGCAGAGAAG 
CAGGGACATC AGTGGAAAGA TTCAGATCCT GTAATGGCTG GAATTGGGGA GGAGATTGCA 
CACTTTCAGA AGGAGTTGGA AGAGTTAAAA GCCCGAACTT CCAAAGCCTG TTTCCAAGTG 
GGCACTTCTG AGGAGATGAA GATGCTGCGA ACAGAATCAG ATGACTTGCA TACCTTTCTT 
TTGGAGATTA AAGAGACCAC AGAGTCGCTT CATGGAGACA TAAGTAGCCT GAAAACAACT 
TTACTTGAGG GCTTTGCTGG TGTTGAGGAA GCCAGAGAAC AAAATGAAAG AAATCGTGAC 
TCTGGTTATC TGCATTTGCT TTATAAAAGA CCACTGGATC CCAAGAGTGA AGCTCAGCTT 
CAGGAAATTC GGCGCCTTCA TCAGTATGTG AAATTTGCTG TCCAAGATGT GAATGATGTT 
CTAGATTTGG AGTGGGATCG GCATCTGGAA CAAAAGAAAA AACAAAGGCA CCTGCTTGTG 
CCAGAGCGAG AGACACTGTT TAACACCCTA GCCAACAACC GGGAAATCAT CAACCAACAG 
AGGAAGAGGC TGAATCACCT GGTGGACAGT CTTCAGCAGC TCCGCCTTTA CAACCAGACT 
TCCCCGTGGA GCCTGCCCTC GGCTGTTCCT TCCCAGAGCA GCATTCACAG TTTTGACAGT 
GACCTTGAAA GCCTGCGCAA TGCTTTGTTG AAAACCACCA TAGAATCTCA CACCAAATCC 
TTGCCCAAAG TACCAGCCAA ACTGTCCCCC ATGAAACAGG CACAACTGAG AAACTTCTTG 
GCCAAGAGGA AGACCCCACC AGTGAGATCC ACTGCTCCAG CCAGCCTGTC TCGATCAGCC 
TTTCTGTCTC AGAGATATTA TGAAGACTTG GATGAAGTCA GCTCAACGTC ATCTGTCTCC 
CAGTCTCTGG AGAGTGAAGA TGCACGGACG TCCTATAAAG ATGAGGAGGC AGTGGTTCAG 
GCCCCTCGGC ACGCCCCCGT GGTTCGCACT CCTTCCGTCC AGCCCAGTCT CTTGCCCCAT 
GCAGCACCTT TTGCTAAATC TCACCTGGTT CATGGTTCTT CACCTGGTGT GATGGGAACT 
TCAGTGGCTA CATCTGCTAG CAAAATTATT CCTCAAGGGG CCGATAGCAC AATGCTTGCC 
ACGAAAACCG TGAAACATGG TGCACCTAGT CCTTCCCACC CCATCTCAGC CCCGCAGGCA 
GCTGCCGCAG CAGCACTCAG GCGGCAGATG GCCAGTCAGG CACCAGCTGT AAACACTTTG 
ACTGAATCAA CGTTGAAGAA TGTCCCTCAA GTGGTAAATG TGCAGGAATT GAAGAATAAC 
CCTGCAACCC CTTCTACAGC CATGGGTTCT TCAGTGGCCT ACTCCGCAGC CAAAACACCT 
CACCCAGTGT TGACCCCAGT GGCCGCTAAC CAAGCCAAGC AGGGGTCTCT AATAAATCCC 
CTTAAGCCAT CTGGGCCTAC ACCAGCATCC GGTCAGTTAT CATCTGGTGA CAAAGCTTCA 
GGGACAGCCA AGATAGAAAC AGCTGTGACT TCGACCCCAT CTGCTTCTGG GCAGTTCAGC 
AAGCCTTTCT CATTTTCTCC ATCAGGGACT GGCTTTAATT TTGGGATAAT CACACCAACA 
CCGTCTTCTA ATTTCACTGC TGCACAAGGG GCAACACCCT CCACTAAAGA GTCAAGCCAG 
CTGGATGCAT TCTCATTTGG TGGGGGAAGC AAACCTTCTT ATGAGGCCAT TCCTGAAAGC 
TCACCTCCCT CAGGAATCAC ATCCGCATCA AACACCACCC CAGGAGAACC TGCCACATCT 
AGCAGCAGAC CTGTGGCACC TTCTGGAACT GCTCTTTCCA CTACCTCTAG TAAGCTGGAA 
ACCCCACCGT CCAAGCTGGG AGAGCTTCTG TTTCCAAGTT CTTTGGCTGG AGAGACTCTG 
GGAAGTTTTT CAGGACTGCG TGTTGGCCAA GCAGATGATT CTACAAAGCC AACCAATAAG 
GCTTCATCCA CAAGCCTAAC TAGTACCCAG CCAACCAAGA CGTCAGGCGT GCCCTCAGGG 
TTTAATTTTA CTGCCCCCCC AGTGTTAGGG AAGCAAACGG AGCCCCCTGT GACCTCCTCT 
GCAACCACCA CCTCAGTAGC ACCACCAGCA GCCACCAGCA CTTCCTCAAC TGGCGTTTTT 
GGCAGTCTGC CGGTCACCAG TGCAGGATCC TCTGGGGTCA TCAGTTTTGG TGGGACATCT 
CTAAGTGGTG GCAAGACTAG TTTTTCATTT GGAAGCCAAC AGACCAATAG CACAGTGCCC 
CCATCTGCCC CACCGCCTAC TACAGCTGCC ACTCCCCTTC CAACATCATT CCCCACATTG 
TCGTTCGGTA GCCTCCTGAG TTCAGCAACT ACCCCCTCCC TGCCTATGTC CTCTGGCAGA 
AGCACAGAAG AGGCCACTTC ATCAGCTTTG CCTGAGAAGC CAGGTGACAG TGAGGTCTCA 
GCATCAGCAG CCTCACTTCT AGAGGAGCAA CAGTCAGCCC AGCTTCCCCA GGCTCCTCCG 
CAAACTTCTG ACTCTGTTAA AAAAGAACCT GCTCTTGCCC AGCCTGCAGT CAGCAACTCT 
GGCACTGCAG CATCTAGTAC TAGTCTTGTA GCACTTTCTG CAGAGGCTAC CCCAACCACC 
ACCGGGGTCC CTGATGCCAG GTCGGAGGCA GTACCACCTG CTTCCTCCTT TTCTGTGCCT 
GGGCAGACTG CTGTCACAGC AGCTGCTATC TCAAGTGCAG GCCCTGTGGC CGTCGAAACA 
TCAAGTGCCC CCATAACCTC CAGCACCACG TCCGTTGTTG CTCCCGGCCC ATCTGCAGAG 
GCAGCAGCAT TTGGTACCGT CACTTCTGGC TCATCAGTCT TTGCTCAGCC CCCTGCTGCC 
AGTTCTAGCT CAGCTTTCAA CCAGCTCACC AACAACACAG CCACTGCCAC TTCTGCCACG 
CCCGTGTTTG GGCAAGTGGC AGCCAGCACT GCACCAAGTC TGTTTGGGCA GCAGACTGGT 
AGCACAGCCA GCACAGCAGC TGCCACACCA CAGGTCAGCA GCTCAGGGTT TAGCAGCCCA 
GCTTTTGGCA CCACAGCCCC AGGGGTCTTT GGACAGACAA CCTTCGGGCA GGCCTCAGTG 
TTTGGGCAGT CAGCGAGCAG TGCCGCAAGT GGCTTTTCCT TCAGTCAGCC TGGGTTCAGT 
TCCGTGCCTG CCTTCGGTCA GCCTGCTTCC TCCACCCCCA CATCCACCAG TGGAAGTGTC 
TTTGGTGCCG CCTCAAGTAC CAGTAGCTCC AGTTCCTTCT CATTTGGACA GTCTTCTGCC 
AACACAGGAG GGGGGCTGTT TGGCCAAAGC AACGCTCCTG CTTTTGGGCA GAGTCCTGGC 
TTTGGACAGG GAGGCTCTGT ATTTGGTGGT ACCTCAGCTG CCACCACAAC AGCAGTATCC 
TCTGGGTTCA GCTTTTGTCA AGCTTCAGGT TTTGGGTCTA GTAATACTGG TTCTGTGTTT 
GGTCAAGCAG CCAGTACTGG TGGAATAGTC TTTGGCCAGC AGTCATCGTC TTCCAGTGGT 
AGCGTGTTTG GGTCTGGAAA CACTGGAAGA GGGGGAGGTT TCTTCAGTGG CCTTGGAGGA 
AAACCCAGTC AGGATGCAGC CAACAAAAAC CCATTCAGCT CGGCCAGTGG GGGCTTTGGA 
TCCACAGCTA CCTCAAATAC CTCTAACCTA TTTGGAAACA GTGGGGCCAA GACATTTGGT 
GGATTTGCCA GCTCGTCGTT TGGAGAGCAG AAACCCACTG GCACTTTCAG CTCTGGAGGA 
GGAAGTGTGG CATCCCAAGG CTTTGGGTTT TCCTCTCCAA ACAAAACAGG TGGCTTCGGT 
GCTGCTCCAG TGTTTGGCAG CCCTCCTACT TTTGGGGGAT CCCCTGGGTT TGGAGGGGTG 
CCAGCATTCG GTTCAGCCCC AGCCTTTACA AGCCCTCTGG GCTCGACGGG AGGCAAAGTG 
TTCGGAGAGG GCACTGCGGC TGCCAGCGCA GGAGGATTCG GGTTTGGGAG CAGCAGCAAC 
ACCACATCCT TCGGCACGCT CGCGAGTCAG AATGCCCCCA CTTTCGGATC ACTGTCCCAA 
CAGACTTCTG GTTTTGGGAC CCAGAGTAGC GGATTCTCTG GTTTTGGATC AGGCACAGGA 
GGGTTCAGCT TTGGATCAAA TAACTCATCT GTCCAGGGTT TTGGTGGCTG GCGAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( XM_024252127.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024107895.1 Pongo abelii nucleoporin 214 (NUP214), transcript variant X6, mRNA.

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CloneID	OSa186581
Clone ID Related Accession (Same CDS sequence)	XM_024252127.1
Accession Version	XM_024252127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	nuclear pore complex protein Nup214 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036912.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTCGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTTGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAATAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGTGTGC ATGATGTCCG GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGGTTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCAATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTC ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGATTAA TTGGGAATCT 
TGGCTACTGG AGGATTCTAG TCGAGCTGAA TTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GAGTTGCCGT AGACTATACG AACCAAGTGG AAATCACCAT CAGTGATGAA 
AAGACTCTTC CTCCTGCTCC AGTTCTCATG TTACTTTCAA CAGATGGTGT GCTTTGTCCA 
TTTTATATGA TTAATCAAAA TCCTGGGGTT AAGTCTCTCA TCAAAACACC AGAGCGACTT 
TCATTAGAAG GAGAGCGACA GCCCAAGTCA CCAGGAAGTA CTCCCACTAC CCCGACCTCC 
TCTCAAGCCC CGCAGAAACT GGATGCTTCT GCAGCTGCAG CCCCTGCCTC TCTTCCACCT 
TCATCACCCG CTGCTCCCAT TGCCACTTTT TCTTTGCTTC CTGCTGGTGG AGCCCCCACT 
GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG TCATCTGCTG CGGTCACTGG GGAGCCCCCT 
TCATATTCCA GTGGCTCCGA CAGCTCCAAA GCAGCCCCGG GCCCTGGCCC ATCAACCTTC 
TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA GCCCCCACCC CTGCAGCGTC TCCCGTGGCT 
CCATCAGCTG CTTCATTCTC GTTTGGATCA TCTGGTTTTA AGCCTACCCT GGAAAGCACA 
CCAGTGCCAA GTGTGTCTGC TCCAAATATA GCAGTGAAGC CCTCCTTCCC ACCCTCAACC 
TCTGCTGTCA AAGTCAACCT TAGTGAAAAG TTTACTGCCG CAGCTACCTC TACTCCTGTT 
AGTAGCTCCC AGAGCGCACC CTCGATGCCG CCATTCTCTT CTGCCTCCAA GCCAGCTACT 
TCTGGACCGC TCAGCCACCC CACGCCTCTC TCAGCATCAC CTAGTTCCGT GCCATTGAAG 
TCCTCAGTCT TGCCCTCACC ATCAGCAGGA CGATCTGCTC AGGGCAGTCC AAGCCCAGTG 
CCCTCAATGG TACAGAAATC ACCCAGGATA ACCCCTCCAG TGGCAAAGCC AGGCTCTCCC 
CAGGCAAAGT CACTTCAGCC TGCTGTTGCA GAGAAGCAGG GACATCAGTG GAAAGATTCA 
GATCCTGTAA TGGCTGGAAT TGGGGAGGAG ATTGCACACT TTCAGAAGGA GTTGGAAGAG 
TTAAAAGCCC GAACTTCCAA AGCCTGTTTC CAAGTGGGCA CTTCTGAGGA GATGAAGATG 
CTGCGAACAG AATCAGATGA CTTGCATACC TTTCTTTTGG AGATTAAAGA GACCACAGAG 
TCGCTTCATG GAGACATAAG TAGCCTGAAA ACAACTTTAC TTGAGGGCTT TGCTGGTGTT 
GAGGAAGCCA GAGAACAAAA TGAAAGAAAT CGTGACTCTG GTTATCTGCA TTTGCTTTAT 
AAAAGACCAC TGGATCCCAA GAGTGAAGCT CAGCTTCAGG AAATTCGGCG CCTTCATCAG 
TATGTGAAAT TTGCTGTCCA AGATGTGAAT GATGTTCTAG ATTTGGAGTG GGATCGGCAT 
CTGGAACAAA AGAAAAAACA AAGGCACCTG CTTGTGCCAG AGCGAGAGAC ACTGTTTAAC 
ACCCTAGCCA ACAACCGGGA AATCATCAAC CAACAGAGGA AGAGGCTGAA TCACCTGGTG 
GACAGTCTTC AGCAGCTCCG CCTTTACAAC CAGACTTCCC CGTGGAGCCT GCCCTCGGCT 
GTTCCTTCCC AGAGCAGCAT TCACAGTTTT GACAGTGACC TTGAAAGCCT GCGCAATGCT 
TTGTTGAAAA CCACCATAGA ATCTCACACC AAATCCTTGC CCAAAGTACC AGCCAAACTG 
TCCCCCATGA AACAGGCACA ACTGAGAAAC TTCTTGGCCA AGAGGAAGAC CCCACCAGTG 
AGATCCACTG CTCCAGCCAG CCTGTCTCGA TCAGCCTTTC TGTCTCAGAG ATATTATGAA 
GACTTGGATG AAGTCAGCTC AACGTCATCT GTCTCCCAGT CTCTGGAGAG TGAAGATGCA 
CGGACGTCCT ATAAAGATGA GGAGGCAGTG GTTCAGGCCC CTCGGCACGC CCCCGTGGTT 
CGCACTCCTT CCGTCCAGCC CAGTCTCTTG CCCCATGCAG CACCTTTTGC TAAATCTCAC 
CTGGTTCATG GTTCTTCACC TGGTGTGATG GGAACTTCAG TGGCTACATC TGCTAGCAAA 
ATTATTCCTC AAGGGGCCGA TAGCACAATG CTTGCCACGA AAACCGTGAA ACATGGTGCA 
CCTAGTCCTT CCCACCCCAT CTCAGCCCCG CAGGCAGCTG CCGCAGCAGC ACTCAGGCGG 
CAGATGGCCA GTCAGGCACC AGCTGTAAAC ACTTTGACTG AATCAACGTT GAAGAATGTC 
CCTCAAGTGG TAAATGTGCA GGAATTGAAG AATAACCCTG CAACCCCTTC TACAGCCATG 
GGTTCTTCAG TGGCCTACTC CGCAGCCAAA ACACCTCACC CAGTGTTGAC CCCAGTGGCC 
GCTAACCAAG CCAAGCAGGG GTCTCTAATA AATCCCCTTA AGCCATCTGG GCCTACACCA 
GCATCCGGTC AGTTATCATC TGGTGACAAA GCTTCAGGGG CAACACCCTC CACTAAAGAG 
TCAAGCCAGC TGGATGCATT CTCATTTGGT GGGGGAAGCA AACCTTCTTA TGAGGCCATT 
CCTGAAAGCT CACCTCCCTC AGGAATCACA TCCGCATCAA ACACCACCCC AGGAGAACCT 
GCCACATCTA GCAGCAGACC TGTGGCACCT TCTGGAACTG CTCTTTCCAC TACCTCTAGT 
AAGCTGGAAA CCCCACCGTC CAAGCTGGGA GAGCTTCTGT TTCCAAGTTC TTTGGCTGGA 
GAGACTCTGG GAAGTTTTTC AGGACTGCGT GTTGGCCAAG CAGATGATTC TACAAAGCCA 
ACCAATAAGG CTTCATCCAC AAGCCTAACT AGTACCCAGC CAACCAAGAC GTCAGGCGTG 
CCCTCAGGGT TTAATTTTAC TGCCCCCCCA GTGTTAGGGA AGCAAACGGA GCCCCCTGTG 
ACCTCCTCTG CAACCACCAC CTCAGTAGCA CCACCAGCAG CCACCAGCAC TTCCTCAACT 
GGCGTTTTTG GCAGTCTGCC GGTCACCAGT GCAGGATCCT CTGGGGTCAT CAGTTTTGGT 
GGGACATCTC TAAGTGGTGG CAAGACTAGT TTTTCATTTG GAAGCCAACA GACCAATAGC 
ACAGTGCCCC CATCTGCCCC ACCGCCTACT ACAGCTGCCA CTCCCCTTCC AACATCATTC 
CCCACATTGT CGTTCGGTAG CCTCCTGAGT TCAGCAACTA CCCCCTCCCT GCCTATGTCC 
TCTGGCAGAA GCACAGAAGA GGCCACTTCA TCAGCTTTGC CTGAGAAGCC AGGTGACAGT 
GAGGTCTCAG CATCAGCAGC CTCACTTCTA GAGGAGCAAC AGTCAGCCCA GCTTCCCCAG 
GCTCCTCCGC AAACTTCTGA CTCTGTTAAA AAAGAACCTG CTCTTGCCCA GCCTGCAGTC 
AGCAACTCTG GCACTGCAGC ATCTAGTACT AGTCTTGTAG CACTTTCTGC AGAGGCTACC 
CCAACCACCA CCGGGGTCCC TGATGCCAGG TCGGAGGCAG TACCACCTGC TTCCTCCTTT 
TCTGTGCCTG GGCAGACTGC TGTCACAGCA GCTGCTATCT CAAGTGCAGG CCCTGTGGCC 
GTCGAAACAT CAAGTGCCCC CATAACCTCC AGCACCACGT CCGTTGTTGC TCCCGGCCCA 
TCTGCAGAGG CAGCAGCATT TGGTACCGTC ACTTCTGGCT CATCAGTCTT TGCTCAGCCC 
CCTGCTGCCA GTTCTAGCTC AGCTTTCAAC CAGCTCACCA ACAACACAGC CACTGCCACT 
TCTGCCACGC CCGTGTTTGG GCAAGTGGCA GCCAGCACTG CACCAAGTCT GTTTGGGCAG 
CAGACTGGTA GCACAGCCAG CACAGCAGCT GCCACACCAC AGGTCAGCAG CTCAGGGTTT 
AGCAGCCCAG CTTTTGGCAC CACAGCCCCA GGGGTCTTTG GACAGACAAC CTTCGGGCAG 
GCCTCAGTGT TTGGGCAGTC AGCGAGCAGT GCCGCAAGTG GCTTTTCCTT CAGTCAGCCT 
GGGTTCAGTT CCGTGCCTGC CTTCGGTCAG CCTGCTTCCT CCACCCCCAC ATCCACCAGT 
GGAAGTGTCT TTGGTGCCGC CTCAAGTACC AGTAGCTCCA GTTCCTTCTC ATTTGGACAG 
TCTTCTGCCA ACACAGGAGG GGGGCTGTTT GGCCAAAGCA ACGCTCCTGC TTTTGGGCAG 
AGTCCTGGCT TTGGACAGGG AGGCTCTGTA TTTGGTGGTA CCTCAGCTGC CACCACAACA 
GCAGTATCCT CTGGGTTCAG CTTTTGTCAA GCTTCAGGTT TTGGGTCTAG TAATACTGGT 
TCTGTGTTTG GTCAAGCAGC CAGTACTGGT GGAATAGTCT TTGGCCAGCA GTCATCGTCT 
TCCAGTGGTA GCGTGTTTGG GTCTGGAAAC ACTGGAAGAG GGGGAGGTTT CTTCAGTGGC 
CTTGGAGGAA AACCCAGTCA GGATGCAGCC AACAAAAACC CATTCAGCTC GGCCAGTGGG 
GGCTTTGGAT CCACAGCTAC CTCAAATACC TCTAACCTAT TTGGAAACAG TGGGGCCAAG 
ACATTTGGTG GATTTGCCAG CTCGTCGTTT GGAGAGCAGA AACCCACTGG CACTTTCAGC 
TCTGGAGGAG GAAGTGTGGC ATCCCAAGGC TTTGGGTTTT CCTCTCCAAA CAAAACAGGT 
GGCTTCGGTG CTGCTCCAGT GTTTGGCAGC CCTCCTACTT TTGGGGGATC CCCTGGGTTT 
GGAGGGGTGC CAGCATTCGG TTCAGCCCCA GCCTTTACAA GCCCTCTGGG CTCGACGGGA 
GGCAAAGTGT TCGGAGAGGG CACTGCGGCT GCCAGCGCAG GAGGATTCGG GTTTGGGAGC 
AGCAGCAACA CCACATCCTT CGGCACGCTC GCGAGTCAGA ATGCCCCCAC TTTCGGATCA 
CTGTCCCAAC AGACTTCTGG TTTTGGGACC CAGAGTAGCG GATTCTCTGG TTTTGGATCA 
GGCACAGGAG GGTTCAGCTT TGGATCAAAT AACTCATCTG TCCAGGGTTT TGGTGGCTGG 
CGAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024107895.1
CDS	139..6267
Translation	MGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSVCMMSGEYGSIIAFFDVRTFSNEAKQQKRPFAYHKLLKDAGGMVVDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQINWESWLLEDSSRAELPVTDKSDDSLPMGVAVDYTNQVEITISDEKTLPPAPVLMLLSTDGVLCPFYMINQNPGVKSLIKTPERLSLEGERQPKSPGSTPTTPTSSQAPQKLDASAAAAPASLPPSSPAAPIATFSLLPAGGAPTVFSFGSSSLKSSAAVTGEPPSYSSGSDSSKAAPGPGPSTFSFVPPSKASLAPTPAASPVAPSAASFSFGSSGFKPTLESTPVPSVSAPNIAVKPSFPPSTSAVKVNLSEKFTAAATSTPVSSSQSAPSMPPFSSASKPATSGPLSHPTPLSASPSSVPLKSSVLPSPSAGRSAQGSPSPVPSMVQKSPRITPPVAKPGSPQAKSLQPAVAEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKARTSKACFQVGTSEEMKMLRTESDDLHTFLLEIKETTESLHGDISSLKTTLLEGFAGVEEAREQNERNRDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDRHLEQKKKQRHLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYNQTSPWSLPSAVPSQSSIHSFDSDLESLRNALLKTTIESHTKSLPKVPAKLSPMKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEVSSTSSVSQSLESEDARTSYKDEEAVVQAPRHAPVVRTPSVQPSLLPHAAPFAKSHLVHGSSPGVMGTSVATSASKIIPQGADSTMLATKTVKHGAPSPSHPISAPQAAAAAALRRQMASQAPAVNTLTESTLKNVPQVVNVQELKNNPATPSTAMGSSVAYSAAKTPHPVLTPVAANQAKQGSLINPLKPSGPTPASGQLSSGDKASGATPSTKESSQLDAFSFGGGSKPSYEAIPESSPPSGITSASNTTPGEPATSSSRPVAPSGTALSTTSSKLETPPSKLGELLFPSSLAGETLGSFSGLRVGQADDSTKPTNKASSTSLTSTQPTKTSGVPSGFNFTAPPVLGKQTEPPVTSSATTTSVAPPAATSTSSTGVFGSLPVTSAGSSGVISFGGTSLSGGKTSFSFGSQQTNSTVPPSAPPPTTAATPLPTSFPTLSFGSLLSSATTPSLPMSSGRSTEEATSSALPEKPGDSEVSASAASLLEEQQSAQLPQAPPQTSDSVKKEPALAQPAVSNSGTAASSTSLVALSAEATPTTTGVPDARSEAVPPASSFSVPGQTAVTAAAISSAGPVAVETSSAPITSSTTSVVAPGPSAEAAAFGTVTSGSSVFAQPPAASSSSAFNQLTNNTATATSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGFSSPAFGTTAPGVFGQTTFGQASVFGQSASSAASGFSFSQPGFSSVPAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSANTGGGLFGQSNAPAFGQSPGFGQGGSVFGGTSAATTTAVSSGFSFCQASGFGSSNTGSVFGQAASTGGIVFGQQSSSSSGSVFGSGNTGRGGGFFSGLGGKPSQDAANKNPFSSASGGFGSTATSNTSNLFGNSGAKTFGGFASSSFGEQKPTGTFSSGGGSVASQGFGFSSPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSNTTSFGTLASQNAPTFGSLSQQTSGFGTQSSGFSGFGSGTGGFSFGSNNSSVQGFGGWRS

Target ORF information:

RefSeq Version	XM_024252127.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii nucleoporin 214 (NUP214), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024252127.1

ORF Insert Sequence:

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTCGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTTGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAATAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGTGTGC ATGATGTCCG GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGGTTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCAATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTC ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGATTAA TTGGGAATCT 
TGGCTACTGG AGGATTCTAG TCGAGCTGAA TTGCCTGTGA CAGACAAGAG TGATGACTCC 
TTGCCCATGG GAGTTGCCGT AGACTATACG AACCAAGTGG AAATCACCAT CAGTGATGAA 
AAGACTCTTC CTCCTGCTCC AGTTCTCATG TTACTTTCAA CAGATGGTGT GCTTTGTCCA 
TTTTATATGA TTAATCAAAA TCCTGGGGTT AAGTCTCTCA TCAAAACACC AGAGCGACTT 
TCATTAGAAG GAGAGCGACA GCCCAAGTCA CCAGGAAGTA CTCCCACTAC CCCGACCTCC 
TCTCAAGCCC CGCAGAAACT GGATGCTTCT GCAGCTGCAG CCCCTGCCTC TCTTCCACCT 
TCATCACCCG CTGCTCCCAT TGCCACTTTT TCTTTGCTTC CTGCTGGTGG AGCCCCCACT 
GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG TCATCTGCTG CGGTCACTGG GGAGCCCCCT 
TCATATTCCA GTGGCTCCGA CAGCTCCAAA GCAGCCCCGG GCCCTGGCCC ATCAACCTTC 
TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA GCCCCCACCC CTGCAGCGTC TCCCGTGGCT 
CCATCAGCTG CTTCATTCTC GTTTGGATCA TCTGGTTTTA AGCCTACCCT GGAAAGCACA 
CCAGTGCCAA GTGTGTCTGC TCCAAATATA GCAGTGAAGC CCTCCTTCCC ACCCTCAACC 
TCTGCTGTCA AAGTCAACCT TAGTGAAAAG TTTACTGCCG CAGCTACCTC TACTCCTGTT 
AGTAGCTCCC AGAGCGCACC CTCGATGCCG CCATTCTCTT CTGCCTCCAA GCCAGCTACT 
TCTGGACCGC TCAGCCACCC CACGCCTCTC TCAGCATCAC CTAGTTCCGT GCCATTGAAG 
TCCTCAGTCT TGCCCTCACC ATCAGCAGGA CGATCTGCTC AGGGCAGTCC AAGCCCAGTG 
CCCTCAATGG TACAGAAATC ACCCAGGATA ACCCCTCCAG TGGCAAAGCC AGGCTCTCCC 
CAGGCAAAGT CACTTCAGCC TGCTGTTGCA GAGAAGCAGG GACATCAGTG GAAAGATTCA 
GATCCTGTAA TGGCTGGAAT TGGGGAGGAG ATTGCACACT TTCAGAAGGA GTTGGAAGAG 
TTAAAAGCCC GAACTTCCAA AGCCTGTTTC CAAGTGGGCA CTTCTGAGGA GATGAAGATG 
CTGCGAACAG AATCAGATGA CTTGCATACC TTTCTTTTGG AGATTAAAGA GACCACAGAG 
TCGCTTCATG GAGACATAAG TAGCCTGAAA ACAACTTTAC TTGAGGGCTT TGCTGGTGTT 
GAGGAAGCCA GAGAACAAAA TGAAAGAAAT CGTGACTCTG GTTATCTGCA TTTGCTTTAT 
AAAAGACCAC TGGATCCCAA GAGTGAAGCT CAGCTTCAGG AAATTCGGCG CCTTCATCAG 
TATGTGAAAT TTGCTGTCCA AGATGTGAAT GATGTTCTAG ATTTGGAGTG GGATCGGCAT 
CTGGAACAAA AGAAAAAACA AAGGCACCTG CTTGTGCCAG AGCGAGAGAC ACTGTTTAAC 
ACCCTAGCCA ACAACCGGGA AATCATCAAC CAACAGAGGA AGAGGCTGAA TCACCTGGTG 
GACAGTCTTC AGCAGCTCCG CCTTTACAAC CAGACTTCCC CGTGGAGCCT GCCCTCGGCT 
GTTCCTTCCC AGAGCAGCAT TCACAGTTTT GACAGTGACC TTGAAAGCCT GCGCAATGCT 
TTGTTGAAAA CCACCATAGA ATCTCACACC AAATCCTTGC CCAAAGTACC AGCCAAACTG 
TCCCCCATGA AACAGGCACA ACTGAGAAAC TTCTTGGCCA AGAGGAAGAC CCCACCAGTG 
AGATCCACTG CTCCAGCCAG CCTGTCTCGA TCAGCCTTTC TGTCTCAGAG ATATTATGAA 
GACTTGGATG AAGTCAGCTC AACGTCATCT GTCTCCCAGT CTCTGGAGAG TGAAGATGCA 
CGGACGTCCT ATAAAGATGA GGAGGCAGTG GTTCAGGCCC CTCGGCACGC CCCCGTGGTT 
CGCACTCCTT CCGTCCAGCC CAGTCTCTTG CCCCATGCAG CACCTTTTGC TAAATCTCAC 
CTGGTTCATG GTTCTTCACC TGGTGTGATG GGAACTTCAG TGGCTACATC TGCTAGCAAA 
ATTATTCCTC AAGGGGCCGA TAGCACAATG CTTGCCACGA AAACCGTGAA ACATGGTGCA 
CCTAGTCCTT CCCACCCCAT CTCAGCCCCG CAGGCAGCTG CCGCAGCAGC ACTCAGGCGG 
CAGATGGCCA GTCAGGCACC AGCTGTAAAC ACTTTGACTG AATCAACGTT GAAGAATGTC 
CCTCAAGTGG TAAATGTGCA GGAATTGAAG AATAACCCTG CAACCCCTTC TACAGCCATG 
GGTTCTTCAG TGGCCTACTC CGCAGCCAAA ACACCTCACC CAGTGTTGAC CCCAGTGGCC 
GCTAACCAAG CCAAGCAGGG GTCTCTAATA AATCCCCTTA AGCCATCTGG GCCTACACCA 
GCATCCGGTC AGTTATCATC TGGTGACAAA GCTTCAGGGG CAACACCCTC CACTAAAGAG 
TCAAGCCAGC TGGATGCATT CTCATTTGGT GGGGGAAGCA AACCTTCTTA TGAGGCCATT 
CCTGAAAGCT CACCTCCCTC AGGAATCACA TCCGCATCAA ACACCACCCC AGGAGAACCT 
GCCACATCTA GCAGCAGACC TGTGGCACCT TCTGGAACTG CTCTTTCCAC TACCTCTAGT 
AAGCTGGAAA CCCCACCGTC CAAGCTGGGA GAGCTTCTGT TTCCAAGTTC TTTGGCTGGA 
GAGACTCTGG GAAGTTTTTC AGGACTGCGT GTTGGCCAAG CAGATGATTC TACAAAGCCA 
ACCAATAAGG CTTCATCCAC AAGCCTAACT AGTACCCAGC CAACCAAGAC GTCAGGCGTG 
CCCTCAGGGT TTAATTTTAC TGCCCCCCCA GTGTTAGGGA AGCAAACGGA GCCCCCTGTG 
ACCTCCTCTG CAACCACCAC CTCAGTAGCA CCACCAGCAG CCACCAGCAC TTCCTCAACT 
GGCGTTTTTG GCAGTCTGCC GGTCACCAGT GCAGGATCCT CTGGGGTCAT CAGTTTTGGT 
GGGACATCTC TAAGTGGTGG CAAGACTAGT TTTTCATTTG GAAGCCAACA GACCAATAGC 
ACAGTGCCCC CATCTGCCCC ACCGCCTACT ACAGCTGCCA CTCCCCTTCC AACATCATTC 
CCCACATTGT CGTTCGGTAG CCTCCTGAGT TCAGCAACTA CCCCCTCCCT GCCTATGTCC 
TCTGGCAGAA GCACAGAAGA GGCCACTTCA TCAGCTTTGC CTGAGAAGCC AGGTGACAGT 
GAGGTCTCAG CATCAGCAGC CTCACTTCTA GAGGAGCAAC AGTCAGCCCA GCTTCCCCAG 
GCTCCTCCGC AAACTTCTGA CTCTGTTAAA AAAGAACCTG CTCTTGCCCA GCCTGCAGTC 
AGCAACTCTG GCACTGCAGC ATCTAGTACT AGTCTTGTAG CACTTTCTGC AGAGGCTACC 
CCAACCACCA CCGGGGTCCC TGATGCCAGG TCGGAGGCAG TACCACCTGC TTCCTCCTTT 
TCTGTGCCTG GGCAGACTGC TGTCACAGCA GCTGCTATCT CAAGTGCAGG CCCTGTGGCC 
GTCGAAACAT CAAGTGCCCC CATAACCTCC AGCACCACGT CCGTTGTTGC TCCCGGCCCA 
TCTGCAGAGG CAGCAGCATT TGGTACCGTC ACTTCTGGCT CATCAGTCTT TGCTCAGCCC 
CCTGCTGCCA GTTCTAGCTC AGCTTTCAAC CAGCTCACCA ACAACACAGC CACTGCCACT 
TCTGCCACGC CCGTGTTTGG GCAAGTGGCA GCCAGCACTG CACCAAGTCT GTTTGGGCAG 
CAGACTGGTA GCACAGCCAG CACAGCAGCT GCCACACCAC AGGTCAGCAG CTCAGGGTTT 
AGCAGCCCAG CTTTTGGCAC CACAGCCCCA GGGGTCTTTG GACAGACAAC CTTCGGGCAG 
GCCTCAGTGT TTGGGCAGTC AGCGAGCAGT GCCGCAAGTG GCTTTTCCTT CAGTCAGCCT 
GGGTTCAGTT CCGTGCCTGC CTTCGGTCAG CCTGCTTCCT CCACCCCCAC ATCCACCAGT 
GGAAGTGTCT TTGGTGCCGC CTCAAGTACC AGTAGCTCCA GTTCCTTCTC ATTTGGACAG 
TCTTCTGCCA ACACAGGAGG GGGGCTGTTT GGCCAAAGCA ACGCTCCTGC TTTTGGGCAG 
AGTCCTGGCT TTGGACAGGG AGGCTCTGTA TTTGGTGGTA CCTCAGCTGC CACCACAACA 
GCAGTATCCT CTGGGTTCAG CTTTTGTCAA GCTTCAGGTT TTGGGTCTAG TAATACTGGT 
TCTGTGTTTG GTCAAGCAGC CAGTACTGGT GGAATAGTCT TTGGCCAGCA GTCATCGTCT 
TCCAGTGGTA GCGTGTTTGG GTCTGGAAAC ACTGGAAGAG GGGGAGGTTT CTTCAGTGGC 
CTTGGAGGAA AACCCAGTCA GGATGCAGCC AACAAAAACC CATTCAGCTC GGCCAGTGGG 
GGCTTTGGAT CCACAGCTAC CTCAAATACC TCTAACCTAT TTGGAAACAG TGGGGCCAAG 
ACATTTGGTG GATTTGCCAG CTCGTCGTTT GGAGAGCAGA AACCCACTGG CACTTTCAGC 
TCTGGAGGAG GAAGTGTGGC ATCCCAAGGC TTTGGGTTTT CCTCTCCAAA CAAAACAGGT 
GGCTTCGGTG CTGCTCCAGT GTTTGGCAGC CCTCCTACTT TTGGGGGATC CCCTGGGTTT 
GGAGGGGTGC CAGCATTCGG TTCAGCCCCA GCCTTTACAA GCCCTCTGGG CTCGACGGGA 
GGCAAAGTGT TCGGAGAGGG CACTGCGGCT GCCAGCGCAG GAGGATTCGG GTTTGGGAGC 
AGCAGCAACA CCACATCCTT CGGCACGCTC GCGAGTCAGA ATGCCCCCAC TTTCGGATCA 
CTGTCCCAAC AGACTTCTGG TTTTGGGACC CAGAGTAGCG GATTCTCTGG TTTTGGATCA 
GGCACAGGAG GGTTCAGCTT TGGATCAAAT AACTCATCTG TCCAGGGTTT TGGTGGCTGG 
CGAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUP214 ( XM_002820311.3 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_002820357.2 Pongo abelii nucleoporin 214kDa (NUP214), mRNA.

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CloneID	OSa08300
Clone ID Related Accession (Same CDS sequence)	XM_002820311.3
Accession Version	XM_002820311.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	nuclear pore complex protein Nup214
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002883812.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 23, 2014 this sequence version replaced XM_002820311.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTCGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTTGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAATAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGTGTGC ATGATGTCCG GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGGTTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCAATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTC ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGAAATA CTTTCTGTTT 
GACTTTGAAA AACTGTCAGA AAGAGCAAAG CACCTTTGCA TACAAGAACC TTCTTTCACG 
TTGTCTTTCT TGCAGGGACA TGGTGATGAA AAGACTCTTC CTCCTGCTCC AGTTCTCATG 
TTACTTTCAA CAGATGGTGT GCTTTGTCCA TTTTATATGA TTAATCAAAA TCCTGGGGTT 
AAGTCTCTCA TCAAAACACC AGAGCGACTT TCATTAGAAG GAGAGCGACA GCCCAAGTCA 
CCAGGAAGTA CTCCCACTAC CCCGACCTCC TCTCAAGCCC CGCAGAAACT GGATGCTTCT 
GCAGCTGCAG CCCCTGCCTC TCTTCCACCT TCATCACCCG CTGCTCCCAT TGCCACTTTT 
TCTTTGCTTC CTGCTGGTGG AGCCCCCACT GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG 
TCATCTGCTG CGGTCACTGG GGAGCCCCCT TCATATTCCA GTGGCTCCGA CAGCTCCAAA 
GCAGCCCCGG GCCCTGGCCC ATCAACCTTC TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA 
GCCCCCACCC CTGCAGCGTC TCCCGTGGCT CCATCAGCTG CTTCATTCTC GTTTGGATCA 
TCTGGTTTTA AGCCTACCCT GGAAAGCACA CCAGTGCCAA GTGTGTCTGC TCCAAATATA 
GCAGTGAAGC CCTCCTTCCC ACCCTCAACC TCTGCTGTCA AAGTCAACCT TAGTGAAAAG 
TTTACTGCCG CAGCTACCTC TACTCCTGTT AGTAGCTCCC AGAGCGCACC CTCGATGCCG 
CCATTCTCTT CTGCCTCCAA GCCAGCTACT TCTGGACCGC TCAGCCACCC CACGCCTCTC 
TCAGCATCAC CTAGTTCCGT GCCATTGAAG TCCTCAGTCT TGCCCTCACC ATCAGGACGA 
TCTGCTCAGG GCAGTCCAAG CCCAGTGCCC TCAATGGTAC AGAAATCACC CAGGATAACC 
CCTCCAGTGG CAAAGCCAGG CTCTCCCCAG GCAAAGTCAC TTCAGCCTGC TGTTGCAGAG 
AAGCAGGGAC ATCAGTGGAA AGATTCAGAT CCTGTAATGG CTGGAATTGG GGAGGAGATT 
GCACACTTTC AGAAGGAGTT GGAAGAGTTA AAAGCCCGAA CTTCCAAAGC CTGTTTCCAA 
GTGGGCACTT CTGAGGAGAT GAAGATGCTG CGAACAGAAT CAGATGACTT GCATACCTTT 
CTTTTGGAGA TTAAAGAGAC CACAGAGTCG CTTCATGGAG ACATAAGTAG CCTGAAAACA 
ACTTTACTTG AGGGCTTTGC TGGTGTTGAG GAAGCCAGAG AACAAAATGA AAGAAATCGT 
GACTCTGGTT ATCTGCATTT GCTTTATAAA AGACCACTGG ATCCCAAGAG TGAAGCTCAG 
CTTCAGGAAA TTCGGCGCCT TCATCAGTAT GTGAAATTTG CTGTCCAAGA TGTGAATGAT 
GTTCTAGATT TGGAGTGGGA TCGGCATCTG GAACAAAAGA AAAAACAAAG GCACTTGCTT 
GTGCCAGAGC GAGAGACACT GTTTAACACC CTAGCCAACA ACCGGGAAAT CATCAACCAA 
CAGAGGAAGA GGCTGAATCA CCTGGTGGAC AGTCTTCAGC AGCTCCGCCT TTACAACCAG 
ACTTCCCCGT GGAGCCTGCC CTCGGCTGTT CCTTCCCAGA GCAGCATTCA CAGTTTTGAC 
AGTGACCTTG AAAGCCTGCG CAATGCTTTG TTGAAAACCA CCATAGAATC TCACACCAAA 
TCCTTGCCCA AAGTACCAGC CAAACTGTCC CCCATGAAAC AGGCACAACT GAGAAACTTC 
TTGGCCAAGA GGAAGACCCC ACCAGTGAGA TCCACTGCTC CAGCCAGCCT GTCTCGATCA 
GCCTTTCTGT CTCAGAGATA TTATGAAGAC TTGGATGAAG TCAGCTCAAC GTCATCTGTC 
TCCCAGTCTC TGGAGAGTGA AGATGCACGG ACGTCCTATA AAGATGAGGA GGCAGTGGTT 
CAGGCCCCTC GGCACGCCCC CGTGGTTCGC ACTCCTTCTG TCCAGCCCAG TCTCTTGCCC 
CATGCAGCAC CTTTTGCTAA ATCTCACCTG GTTCATGGTT CTTCACCTGG TGTGATGGGA 
ACTTCAGTGG CTACATCTGC TAGCAAAATT ATTCCTCAAG GGGCCGATAG CACAATGCTT 
GCCACGAAAA CCGTGAAACA TGGTGCACCT AGTCCTTCCC ACCCCATCTC AGCCCCGCAG 
GCAGCTGCAG CAGCAGCACT CAGGCGGCAG ATGGCCAGTC AGGCACCAGC TGTAAACACT 
TTGACTGAAT CAACGTTGAA GAATGTCCCT CAAGTGGTAA ATGTGCAGGA ATTGAAGAAT 
AACCCTGCAA CCCCTTCTAC AGCCATGGGT TCTTCAGTGG CCTACTCCGC AGCCAAAACA 
CCTCACCCAG TGTTGACCCC AGTGGCCGCT AACCAAGCCA AGCAGGGGTC TCTAATAAAT 
CCCCTTAAGC CATCTGGGCC TACACCAGCA TCCGGTCAGT TATCATCTGG TGACAAAGCT 
TCAGGGACAG CCAAGATAGA AACAGCTGTG ACTTCGACCC CATCTGCTTC TGGGCAGTTC 
AGCAAGCCTT TCTCATTTTC TCCATCAGGG ACTGGCTTTA ATTTTGGGAT AATCACACCA 
ACACCGTCTT CTAATTTCAC TGCTGCACAA GGGGCAACAC CCTCCACTAA AGAGTCAAGC 
CAGCTGGATG CATTCTCATT TGGTGGGGGA AGCAAACCTT CTTATGAGGC CATTCCTGAA 
AGCTCACCTC CCTCAGGAAT CACATCCGCA TCAAACACCA CCCCAGGAGA ACCTGCCACA 
TCTAGCAGCA GACCTGTGGC ACCTTCTGGA ACTGCTCTTT CCACTACCTC TAGTAAGCTG 
GAAACCCCAC CGTCCAAGCT GGGAGAGCTT CTGTTTCCAA GTTCTTTGGC TGGAGAGACT 
CTGGGAAGTT TTTCAGGACT GCGTGTTGGC CAAGCAGATG ATTCTACAAA GCCAACCAAT 
AAGGCTTCAT CCACAAGCCT AACTAGTACC CAGCCAACCA AGACGTCAGG CGTGCCCTCA 
GGGTTTAATT TTACTGCCCC CCCAGTGTTA GGGAAGCAAA CGGAGCCCCC TGTGACCTCC 
TCTGCAACCA CCACCTCAGT AGCACCACCA GCAGCCACCA GCACTTCCTC AACTGGCGTT 
TTTGGCAGTC TGCCGGTCAC CAGTGCAGGA TCCTCTGGGG TCATCAGTTT TGGTGGGACA 
TCTCTAAGTG GTGGCAAGAC TAGTTTTTCA TTTGGAAGCC AACAGACCAA TAGCACAGTG 
CCCCCATCTG CCCCACCGCC TACTACAGCT GCCACTCCCC TTCCAACATC ATTCCCCACA 
TTGTCGTTCG GTAGCCTCCT GAGTTCAGCA ACTACCCCCT CCCTGCCTAT GTCCTCTGGC 
AGAAGCACAG AAGAGGCCAC TTCATCAGCT TTGCCTGAGA AGCCAGGTGA CAGTGAGGTC 
TCAGCATCAG CAGCCTCACT TCTAGAGGAG CAACAGTCAG CCCAGCTTCC CCAGGCTCCT 
CCGCAAACTT CTGACTCTGT TAAAAAAGAA CCTGCTCTTG CCCAGCCTGC AGTCAGCAAC 
TCTGGCACTG CAGCATCTAG TACTAGTCTT GTAGCACTTT CTGCAGAGGC TACCCCAACC 
ACCACCGGGG TCCCTGATGC CAGGTCGGAG GCAGTACCAC CTGCTTCCTC CTTTTCTGTG 
CCTGGGCAGA CTGCTGTCAC AGCAGCTGCT ATCTCAAGTG CAGGCCCTGT GGCCGTCGAA 
ACATCAAGTG CCCCCATAAC CTCCAGCACC ACGTCCGTTG TTGCTCCCGG CCCATCTGCA 
GAGGCAGCAG CATTTGGTAC CGTCACTTCT GGCTCATCAG TCTTTGCTCA GCCCCCTGCT 
GCCAGTTCTA GCTCAGCTTT CAACCAGCTC ACCAACAACA CAGCCACTGC CACTTCTGCC 
ACGCCCGTGT TTGGGCAAGT GGCAGCCAGC ACTGCACCAA GTCTGTTTGG GCAGCAGACT 
GGTAGCACAG CCAGCACAGC AGCTGCCACA CCACAGGTCA GCAGCTCAGG GTTTAGCAGC 
CCAGCTTTTG GCACCACAGC CCCAGGGGTC TTTGGACAGA CAACCTTCGG GCAGGCCTCA 
GTGTTTGGGC AGTCAGCGAG CAGTGCCGCA AGTGGCTTTT CCTTCAGTCA GCCTGGGTTC 
AGTTCCGTGC CTGCCTTCGG TCAGCCTGCT TCCTCCACCC CCACATCCAC CAGTGGAAGT 
GTCTTTGGTG CCGCCTCAAG TACCAGTAGC TCCAGTTCCT TCTCATTTGG ACAGTCTTCT 
GCCAACACAG GAGGGGGGCT GTTTGGCCAA AGCAACGCTC CTGCTTTTGG GCAGAGTCCT 
GGCTTTGGAC AGGGAGGCTC TGTATTTGGT GGTACCTCAG CTGCCACCAC AACAGCAGTA 
TCCTCTGGGT TCAGCTTTTG TCAAGCTTCA GGTTTTGGGT CTAGTAATAC TGGTTCTGTG 
TTTGGTCAAG CAGCCAGTAC TGGTGGAATA GTCTTTGGCC AGCAGTCATC GTCTTCCAGT 
GGTAGCGTGT TTGGGTCTGG AAACACTGGA AGAGGGGGAG GTTTCTTCAG TGGCCTTGGA 
GGAAAACCCA GTCAGGATGC AGCCAACAAA AACCCATTCA GCTCGGCCAG TGGGGGCTTT 
GGATCCACAG CTACCTCAAA TACCTCTAAC CTATTTGGAA ACAGTGGGGC CAAGACATTT 
GGTGGATTTG CCAGCTCGTC GTTTGGAGAG CAGAAACCCA CTGGCACTTT CAGCTCTGGA 
GGAGGAAGTG TGGCATCCCA AGGCTTTGGG TTTTCCTCTC CAAACAAAAC AGGTGGCTTC 
GGTGCTGCTC CAGTGTTTGG CAGCCCTCCT ACTTTTGGGG GATCCCCTGG GTTTGGAGGG 
GTGCCAGCAT TCGGTTCAGC CCCAGCCTTT ACAAGCCCTC TGGGCTCGAC GGGAGGCAAA 
GTGTTCGGAG AGGGCACTGC GGCTGCCAGC GCAGGAGGAT TCGGGTTTGG GAGCAGCAGC 
AACACCACAT CCTTCGGCAC GCTCGCGAGT CAGAATGCCC CCACTTTCGG ATCACTGTCC 
CAACAGACTT CTGGTTTTGG GACCCAGAGT AGCGGATTCT CTGGTTTTGG ATCAGGCACA 
GGAGGGTTCA GCTTTGGATC AAATAACTCA TCTGTCCAGG GTTTTGGTGG CTGGCGAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002820357.2
CDS	139..6381
Translation	MGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSVCMMSGEYGSIIAFFDVRTFSNEAKQQKRPFAYHKLLKDAGGMVVDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQKYFLFDFEKLSERAKHLCIQEPSFTLSFLQGHGDEKTLPPAPVLMLLSTDGVLCPFYMINQNPGVKSLIKTPERLSLEGERQPKSPGSTPTTPTSSQAPQKLDASAAAAPASLPPSSPAAPIATFSLLPAGGAPTVFSFGSSSLKSSAAVTGEPPSYSSGSDSSKAAPGPGPSTFSFVPPSKASLAPTPAASPVAPSAASFSFGSSGFKPTLESTPVPSVSAPNIAVKPSFPPSTSAVKVNLSEKFTAAATSTPVSSSQSAPSMPPFSSASKPATSGPLSHPTPLSASPSSVPLKSSVLPSPSGRSAQGSPSPVPSMVQKSPRITPPVAKPGSPQAKSLQPAVAEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKARTSKACFQVGTSEEMKMLRTESDDLHTFLLEIKETTESLHGDISSLKTTLLEGFAGVEEAREQNERNRDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDRHLEQKKKQRHLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYNQTSPWSLPSAVPSQSSIHSFDSDLESLRNALLKTTIESHTKSLPKVPAKLSPMKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEVSSTSSVSQSLESEDARTSYKDEEAVVQAPRHAPVVRTPSVQPSLLPHAAPFAKSHLVHGSSPGVMGTSVATSASKIIPQGADSTMLATKTVKHGAPSPSHPISAPQAAAAAALRRQMASQAPAVNTLTESTLKNVPQVVNVQELKNNPATPSTAMGSSVAYSAAKTPHPVLTPVAANQAKQGSLINPLKPSGPTPASGQLSSGDKASGTAKIETAVTSTPSASGQFSKPFSFSPSGTGFNFGIITPTPSSNFTAAQGATPSTKESSQLDAFSFGGGSKPSYEAIPESSPPSGITSASNTTPGEPATSSSRPVAPSGTALSTTSSKLETPPSKLGELLFPSSLAGETLGSFSGLRVGQADDSTKPTNKASSTSLTSTQPTKTSGVPSGFNFTAPPVLGKQTEPPVTSSATTTSVAPPAATSTSSTGVFGSLPVTSAGSSGVISFGGTSLSGGKTSFSFGSQQTNSTVPPSAPPPTTAATPLPTSFPTLSFGSLLSSATTPSLPMSSGRSTEEATSSALPEKPGDSEVSASAASLLEEQQSAQLPQAPPQTSDSVKKEPALAQPAVSNSGTAASSTSLVALSAEATPTTTGVPDARSEAVPPASSFSVPGQTAVTAAAISSAGPVAVETSSAPITSSTTSVVAPGPSAEAAAFGTVTSGSSVFAQPPAASSSSAFNQLTNNTATATSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGFSSPAFGTTAPGVFGQTTFGQASVFGQSASSAASGFSFSQPGFSSVPAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSANTGGGLFGQSNAPAFGQSPGFGQGGSVFGGTSAATTTAVSSGFSFCQASGFGSSNTGSVFGQAASTGGIVFGQQSSSSSGSVFGSGNTGRGGGFFSGLGGKPSQDAANKNPFSSASGGFGSTATSNTSNLFGNSGAKTFGGFASSSFGEQKPTGTFSSGGGSVASQGFGFSSPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSNTTSFGTLASQNAPTFGSLSQQTSGFGTQSSGFSGFGSGTGGFSFGSNNSSVQGFGGWRS

Target ORF information:

RefSeq Version	XM_002820311.3
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii nucleoporin 214kDa (NUP214), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002820311.3

ORF Insert Sequence:

ATGGGAGACG AGATGGATGC CATGATTCCC GAGCGGGAGA TGAAGGATTT TCAGTTTAGA 
GCGCTAAAGA AGGTGAGAAT CTTCGACTCC CCTGAGGAAT TGCCCAAGGA ACGCTCGAGT 
CTGCTTGCTG TGTCCAACAA ATATGGTCTG GTCTTTGCTG GTGGAGCCAG TGGCTTGCAG 
ATTTTTCCTA CTAAAAATCT TCTTATTCAA AATAAACCCG GAGATGATCC CAATAAAATA 
GTTGATAAAG TCCAAGGCTT GCTAGTTCCT ATGAAATTCC CAATCCATCA CCTGGCCTTG 
AGCTGTGATA ACCTCACACT CTCTGTGTGC ATGATGTCCG GTGAATATGG TTCCATTATT 
GCTTTTTTTG ATGTTCGCAC ATTCTCAAAT GAGGCTAAAC AGCAAAAACG CCCATTTGCC 
TATCATAAGC TTTTGAAAGA TGCAGGAGGC ATGGTGGTTG ATATGAAGTG GAACCCCACT 
GTCCCCTCCA TGGTGGCAGT TTGTCTGGCT GATGGTAGTA TTGCTGTCCT GCAAGTCACG 
GAAACAGTGA AAGTATGTGC AACTCTTCCT TCCACGGTAG CAGTAACCTC TGTGTGCTGG 
AGCCCCAAAG GAAAGCAGCT GGCAGTGGGA AAACAGAATG GAACTGTGGT CCAGTATCTT 
CCTACTTTGC AGGAAAAAAA AGTCATTCCT TGTCCTCCGT TTTATGAGTC AGATCATCCT 
GTCAGAGTTC TGGATGTGCT GTGGATTGGT ACCTACGTCT TCGCAATAGT GTATGCTGCT 
GCAGATGGGA CCCTGGAAAC GTCTCCAGAT GTGGTGATGG CTCTACTACC GAAAAAAGAA 
GAAAAGCACC CAGAGATATT TGTGAACTTC ATGGAGCCCT GTTATGGCAG CTGCACGGAG 
AGACAGCATC ATTACTACCT CAGTTACATT GAGGAATGGG ATTTAGTGCT GGCAGCATCT 
GCGGCTTCAA CAGAAGTTAG TATCCTTGCT CGACAAAGTG ATCAGAAATA CTTTCTGTTT 
GACTTTGAAA AACTGTCAGA AAGAGCAAAG CACCTTTGCA TACAAGAACC TTCTTTCACG 
TTGTCTTTCT TGCAGGGACA TGGTGATGAA AAGACTCTTC CTCCTGCTCC AGTTCTCATG 
TTACTTTCAA CAGATGGTGT GCTTTGTCCA TTTTATATGA TTAATCAAAA TCCTGGGGTT 
AAGTCTCTCA TCAAAACACC AGAGCGACTT TCATTAGAAG GAGAGCGACA GCCCAAGTCA 
CCAGGAAGTA CTCCCACTAC CCCGACCTCC TCTCAAGCCC CGCAGAAACT GGATGCTTCT 
GCAGCTGCAG CCCCTGCCTC TCTTCCACCT TCATCACCCG CTGCTCCCAT TGCCACTTTT 
TCTTTGCTTC CTGCTGGTGG AGCCCCCACT GTGTTCTCCT TTGGTTCTTC ATCTTTGAAG 
TCATCTGCTG CGGTCACTGG GGAGCCCCCT TCATATTCCA GTGGCTCCGA CAGCTCCAAA 
GCAGCCCCGG GCCCTGGCCC ATCAACCTTC TCTTTTGTTC CCCCTTCTAA AGCCTCCCTA 
GCCCCCACCC CTGCAGCGTC TCCCGTGGCT CCATCAGCTG CTTCATTCTC GTTTGGATCA 
TCTGGTTTTA AGCCTACCCT GGAAAGCACA CCAGTGCCAA GTGTGTCTGC TCCAAATATA 
GCAGTGAAGC CCTCCTTCCC ACCCTCAACC TCTGCTGTCA AAGTCAACCT TAGTGAAAAG 
TTTACTGCCG CAGCTACCTC TACTCCTGTT AGTAGCTCCC AGAGCGCACC CTCGATGCCG 
CCATTCTCTT CTGCCTCCAA GCCAGCTACT TCTGGACCGC TCAGCCACCC CACGCCTCTC 
TCAGCATCAC CTAGTTCCGT GCCATTGAAG TCCTCAGTCT TGCCCTCACC ATCAGGACGA 
TCTGCTCAGG GCAGTCCAAG CCCAGTGCCC TCAATGGTAC AGAAATCACC CAGGATAACC 
CCTCCAGTGG CAAAGCCAGG CTCTCCCCAG GCAAAGTCAC TTCAGCCTGC TGTTGCAGAG 
AAGCAGGGAC ATCAGTGGAA AGATTCAGAT CCTGTAATGG CTGGAATTGG GGAGGAGATT 
GCACACTTTC AGAAGGAGTT GGAAGAGTTA AAAGCCCGAA CTTCCAAAGC CTGTTTCCAA 
GTGGGCACTT CTGAGGAGAT GAAGATGCTG CGAACAGAAT CAGATGACTT GCATACCTTT 
CTTTTGGAGA TTAAAGAGAC CACAGAGTCG CTTCATGGAG ACATAAGTAG CCTGAAAACA 
ACTTTACTTG AGGGCTTTGC TGGTGTTGAG GAAGCCAGAG AACAAAATGA AAGAAATCGT 
GACTCTGGTT ATCTGCATTT GCTTTATAAA AGACCACTGG ATCCCAAGAG TGAAGCTCAG 
CTTCAGGAAA TTCGGCGCCT TCATCAGTAT GTGAAATTTG CTGTCCAAGA TGTGAATGAT 
GTTCTAGATT TGGAGTGGGA TCGGCATCTG GAACAAAAGA AAAAACAAAG GCACTTGCTT 
GTGCCAGAGC GAGAGACACT GTTTAACACC CTAGCCAACA ACCGGGAAAT CATCAACCAA 
CAGAGGAAGA GGCTGAATCA CCTGGTGGAC AGTCTTCAGC AGCTCCGCCT TTACAACCAG 
ACTTCCCCGT GGAGCCTGCC CTCGGCTGTT CCTTCCCAGA GCAGCATTCA CAGTTTTGAC 
AGTGACCTTG AAAGCCTGCG CAATGCTTTG TTGAAAACCA CCATAGAATC TCACACCAAA 
TCCTTGCCCA AAGTACCAGC CAAACTGTCC CCCATGAAAC AGGCACAACT GAGAAACTTC 
TTGGCCAAGA GGAAGACCCC ACCAGTGAGA TCCACTGCTC CAGCCAGCCT GTCTCGATCA 
GCCTTTCTGT CTCAGAGATA TTATGAAGAC TTGGATGAAG TCAGCTCAAC GTCATCTGTC 
TCCCAGTCTC TGGAGAGTGA AGATGCACGG ACGTCCTATA AAGATGAGGA GGCAGTGGTT 
CAGGCCCCTC GGCACGCCCC CGTGGTTCGC ACTCCTTCTG TCCAGCCCAG TCTCTTGCCC 
CATGCAGCAC CTTTTGCTAA ATCTCACCTG GTTCATGGTT CTTCACCTGG TGTGATGGGA 
ACTTCAGTGG CTACATCTGC TAGCAAAATT ATTCCTCAAG GGGCCGATAG CACAATGCTT 
GCCACGAAAA CCGTGAAACA TGGTGCACCT AGTCCTTCCC ACCCCATCTC AGCCCCGCAG 
GCAGCTGCAG CAGCAGCACT CAGGCGGCAG ATGGCCAGTC AGGCACCAGC TGTAAACACT 
TTGACTGAAT CAACGTTGAA GAATGTCCCT CAAGTGGTAA ATGTGCAGGA ATTGAAGAAT 
AACCCTGCAA CCCCTTCTAC AGCCATGGGT TCTTCAGTGG CCTACTCCGC AGCCAAAACA 
CCTCACCCAG TGTTGACCCC AGTGGCCGCT AACCAAGCCA AGCAGGGGTC TCTAATAAAT 
CCCCTTAAGC CATCTGGGCC TACACCAGCA TCCGGTCAGT TATCATCTGG TGACAAAGCT 
TCAGGGACAG CCAAGATAGA AACAGCTGTG ACTTCGACCC CATCTGCTTC TGGGCAGTTC 
AGCAAGCCTT TCTCATTTTC TCCATCAGGG ACTGGCTTTA ATTTTGGGAT AATCACACCA 
ACACCGTCTT CTAATTTCAC TGCTGCACAA GGGGCAACAC CCTCCACTAA AGAGTCAAGC 
CAGCTGGATG CATTCTCATT TGGTGGGGGA AGCAAACCTT CTTATGAGGC CATTCCTGAA 
AGCTCACCTC CCTCAGGAAT CACATCCGCA TCAAACACCA CCCCAGGAGA ACCTGCCACA 
TCTAGCAGCA GACCTGTGGC ACCTTCTGGA ACTGCTCTTT CCACTACCTC TAGTAAGCTG 
GAAACCCCAC CGTCCAAGCT GGGAGAGCTT CTGTTTCCAA GTTCTTTGGC TGGAGAGACT 
CTGGGAAGTT TTTCAGGACT GCGTGTTGGC CAAGCAGATG ATTCTACAAA GCCAACCAAT 
AAGGCTTCAT CCACAAGCCT AACTAGTACC CAGCCAACCA AGACGTCAGG CGTGCCCTCA 
GGGTTTAATT TTACTGCCCC CCCAGTGTTA GGGAAGCAAA CGGAGCCCCC TGTGACCTCC 
TCTGCAACCA CCACCTCAGT AGCACCACCA GCAGCCACCA GCACTTCCTC AACTGGCGTT 
TTTGGCAGTC TGCCGGTCAC CAGTGCAGGA TCCTCTGGGG TCATCAGTTT TGGTGGGACA 
TCTCTAAGTG GTGGCAAGAC TAGTTTTTCA TTTGGAAGCC AACAGACCAA TAGCACAGTG 
CCCCCATCTG CCCCACCGCC TACTACAGCT GCCACTCCCC TTCCAACATC ATTCCCCACA 
TTGTCGTTCG GTAGCCTCCT GAGTTCAGCA ACTACCCCCT CCCTGCCTAT GTCCTCTGGC 
AGAAGCACAG AAGAGGCCAC TTCATCAGCT TTGCCTGAGA AGCCAGGTGA CAGTGAGGTC 
TCAGCATCAG CAGCCTCACT TCTAGAGGAG CAACAGTCAG CCCAGCTTCC CCAGGCTCCT 
CCGCAAACTT CTGACTCTGT TAAAAAAGAA CCTGCTCTTG CCCAGCCTGC AGTCAGCAAC 
TCTGGCACTG CAGCATCTAG TACTAGTCTT GTAGCACTTT CTGCAGAGGC TACCCCAACC 
ACCACCGGGG TCCCTGATGC CAGGTCGGAG GCAGTACCAC CTGCTTCCTC CTTTTCTGTG 
CCTGGGCAGA CTGCTGTCAC AGCAGCTGCT ATCTCAAGTG CAGGCCCTGT GGCCGTCGAA 
ACATCAAGTG CCCCCATAAC CTCCAGCACC ACGTCCGTTG TTGCTCCCGG CCCATCTGCA 
GAGGCAGCAG CATTTGGTAC CGTCACTTCT GGCTCATCAG TCTTTGCTCA GCCCCCTGCT 
GCCAGTTCTA GCTCAGCTTT CAACCAGCTC ACCAACAACA CAGCCACTGC CACTTCTGCC 
ACGCCCGTGT TTGGGCAAGT GGCAGCCAGC ACTGCACCAA GTCTGTTTGG GCAGCAGACT 
GGTAGCACAG CCAGCACAGC AGCTGCCACA CCACAGGTCA GCAGCTCAGG GTTTAGCAGC 
CCAGCTTTTG GCACCACAGC CCCAGGGGTC TTTGGACAGA CAACCTTCGG GCAGGCCTCA 
GTGTTTGGGC AGTCAGCGAG CAGTGCCGCA AGTGGCTTTT CCTTCAGTCA GCCTGGGTTC 
AGTTCCGTGC CTGCCTTCGG TCAGCCTGCT TCCTCCACCC CCACATCCAC CAGTGGAAGT 
GTCTTTGGTG CCGCCTCAAG TACCAGTAGC TCCAGTTCCT TCTCATTTGG ACAGTCTTCT 
GCCAACACAG GAGGGGGGCT GTTTGGCCAA AGCAACGCTC CTGCTTTTGG GCAGAGTCCT 
GGCTTTGGAC AGGGAGGCTC TGTATTTGGT GGTACCTCAG CTGCCACCAC AACAGCAGTA 
TCCTCTGGGT TCAGCTTTTG TCAAGCTTCA GGTTTTGGGT CTAGTAATAC TGGTTCTGTG 
TTTGGTCAAG CAGCCAGTAC TGGTGGAATA GTCTTTGGCC AGCAGTCATC GTCTTCCAGT 
GGTAGCGTGT TTGGGTCTGG AAACACTGGA AGAGGGGGAG GTTTCTTCAG TGGCCTTGGA 
GGAAAACCCA GTCAGGATGC AGCCAACAAA AACCCATTCA GCTCGGCCAG TGGGGGCTTT 
GGATCCACAG CTACCTCAAA TACCTCTAAC CTATTTGGAA ACAGTGGGGC CAAGACATTT 
GGTGGATTTG CCAGCTCGTC GTTTGGAGAG CAGAAACCCA CTGGCACTTT CAGCTCTGGA 
GGAGGAAGTG TGGCATCCCA AGGCTTTGGG TTTTCCTCTC CAAACAAAAC AGGTGGCTTC 
GGTGCTGCTC CAGTGTTTGG CAGCCCTCCT ACTTTTGGGG GATCCCCTGG GTTTGGAGGG 
GTGCCAGCAT TCGGTTCAGC CCCAGCCTTT ACAAGCCCTC TGGGCTCGAC GGGAGGCAAA 
GTGTTCGGAG AGGGCACTGC GGCTGCCAGC GCAGGAGGAT TCGGGTTTGG GAGCAGCAGC 
AACACCACAT CCTTCGGCAC GCTCGCGAGT CAGAATGCCC CCACTTTCGG ATCACTGTCC 
CAACAGACTT CTGGTTTTGG GACCCAGAGT AGCGGATTCT CTGGTTTTGG ATCAGGCACA 
GGAGGGTTCA GCTTTGGATC AAATAACTCA TCTGTCCAGG GTTTTGGTGG CTGGCGAAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NUP214 cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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