ESPL1 cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene
Clones
gRNAs

Summary

Gene Symbol	ESPL1
Entrez Gene ID	100444516
Full Name	extra spindle pole bodies like 1, separase
Gene Type	protein-coding
Organism	Pongo abelii(Sumatran orangutan)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024257504.1	XP_024113272.1	separin isoform X1
XM_024257509.1	XP_024113277.1	separin isoform X3
XM_009247812.1	XP_009246087.1	separin
XM_024257505.1	XP_024113273.1	separin isoform X1
XM_024257506.1	XP_024113274.1	separin isoform X1
XM_024257507.1	XP_024113275.1	separin isoform X1
XM_024257508.1	XP_024113276.1	separin isoform X2

Pathways from BioSystems
Gene Ontology

The following ESPL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ESPL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa168216	XM_024257509.1 Latest version!	Pongo abelii extra spindle pole bodies like 1, separase (ESPL1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa168214	XM_024257507.1 Latest version!	Pongo abelii extra spindle pole bodies like 1, separase (ESPL1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa168214	XM_024257504.1 Latest version!	Pongo abelii extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa168214	XM_024257506.1 Latest version!	Pongo abelii extra spindle pole bodies like 1, separase (ESPL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa168214	XM_024257505.1 Latest version!	Pongo abelii extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa168215	XM_024257508.1 Latest version!	Pongo abelii extra spindle pole bodies like 1, separase (ESPL1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSa10466	XM_009247812.1 Latest version!	Pongo abelii extra spindle pole bodies homolog 1 (S. cerevisiae) (ESPL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ESPL1 ( XM_024257509.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024113277.1 Pongo abelii extra spindle pole bodies like 1, separase (ESPL1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OSa168216
Clone ID Related Accession (Same CDS sequence)	XM_024257509.1
Accession Version	XM_024257509.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	separin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036915.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCGGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTGGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC AATCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAGA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTACAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT ACGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATATA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGAGAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACTACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCTGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAACCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA AGAGCTGCAG GCTTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCTGGGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GAGTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGCAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTACTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGCGCCTGA AGCATCTCAA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACTGAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAGCCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CGCCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAGG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGCG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGGTA GTGCCCCTGG GAACCCTGGC CTGAGTGGCA GGAGCTGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCAGCGT CCCCGGAGGG CCAATGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAGGAACTGA CTGACAACTG GAGAGAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGATGGG GAAGACTCAG CCTCAGGTGG GAAGGCTCCA 
GCGCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAACTGC CCAGCCCATG CCCAGACAAG GACAGTGACA AGGACCTTGG TCCTCGGCTC 
CAGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC GTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGACCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GAGGCTGAGC 
CTTCAGGAGA CGCCTAGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGGAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCCTCTG CGTTCAGTCC TGAATGAATT CGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACGGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGAATGTGGC TGGAAATATC CTGACCCCAC TCTGCTGAAA 
ATCATGCTCA GTGGTGCCGG TGCCCTCACC CCTCAGGACA TTCAGGCCCT GGCCTACGGG 
CTGTGCCCAA CCCAGCCAAA GCGAGCCCAG GAGCTCCTGA ATGAGGCAGT AGGACGGCTA 
CAGGGCCTGA CAGTACCAGG CAATAGCCAC CTTGTCTTGG TCCTAGACAA GGACTTGCAG 
AAGCTGCCGT GGGAAAGCAT GCCCAGCCTC CAGGCACTGC CTGTCACCCG GCTGCCCTCC 
TTCCGCTTCC TACTCAGCTA CTCCATCATC AAAGAGTATG GGGCCTCGCC AGTGCTGAGT 
CAAGGGGTGA ATCCGCGAAG TACCTTCTAT GTCCTGAACC CTCACAATAA CCTGTCAAGC 
ACAGAGGAGC AATTTCGAGC CAATTTCAGC AGTGAAGCTG GCTGGAGAGG AGTGGTTGGG 
GAGGTGCCAA GACCTGAACA GGTGCAAGAA GCCCTGACAA AGCATGATTT GTATATCTAT 
GCAGGGCATG GGGCTGGTGC CCGCTTCCTT GACGGGCAGG CTGTCCTGCG GCTGAGCTGT 
CGGGCAGTGG CCCTGCTGTT TGGCTGTAGC AGTGCGGCCC TGGCTGTGCA TGGAAACCTG 
GAGGGGGCTG GCATTGTGCT CAAGTACATC ATGGCTGGTT GCCCCTTGTT TCTGGGTAAT 
CTCTGGGATG TGACTGACCG TGACATTGAC CGCTACACGG AAGCTCTGCT GCAGGGCTGG 
CTTGGAGCGG GCCCAGGGGC CCCCCTTCTC TACTATGTAA ACCAGGCCCG CCAAGCTCCC 
CGACTCAAGT ATCTTATTGG GGCTGCACCT ATAGCCTATG GCTTGCCTGT CTCTCTGCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024113277.1
CDS	138..5360
Translation	MRSFKRVNFGTLLSSRKEAEELLPDLKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATIRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRVVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDRAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQIYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVESCKSTVVWMLEALEGLSGQELTDYMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDNLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPGETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLESVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRAQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASRLKHLNQTTDTYLLLSLTCDLLRSQLYWTHQKVTEGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNSLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTGSAPGNPGLSGRSWRAKKVASRHCEEQRPRRANDQARPGPEIMRTIPEEELTDNWREMSFEILRGSDGEDSASGGKAPAPGPEAASGEWELLRLDSSKKELPSPCPDKDSDKDLGPRLQLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKTQKHRGSLEIADQLQRLSLQETPRDVPLARIQRLFSFRALGSGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLPLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQECGWKYPDPTLLKIMLSGAGALTPQDIQALAYGLCPTQPKRAQELLNEAVGRLQGLTVPGNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVNPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR

Target ORF information:

RefSeq Version	XM_024257509.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii extra spindle pole bodies like 1, separase (ESPL1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024257509.1

ORF Insert Sequence:

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCGGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTGGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC AATCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAGA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTACAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT ACGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATATA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGAGAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACTACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCTGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAACCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA AGAGCTGCAG GCTTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCTGGGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GAGTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGCAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTACTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGCGCCTGA AGCATCTCAA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACTGAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAGCCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CGCCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAGG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGCG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGGTA GTGCCCCTGG GAACCCTGGC CTGAGTGGCA GGAGCTGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCAGCGT CCCCGGAGGG CCAATGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAGGAACTGA CTGACAACTG GAGAGAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGATGGG GAAGACTCAG CCTCAGGTGG GAAGGCTCCA 
GCGCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAACTGC CCAGCCCATG CCCAGACAAG GACAGTGACA AGGACCTTGG TCCTCGGCTC 
CAGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC GTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGACCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GAGGCTGAGC 
CTTCAGGAGA CGCCTAGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGGAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCCTCTG CGTTCAGTCC TGAATGAATT CGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACGGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGAATGTGGC TGGAAATATC CTGACCCCAC TCTGCTGAAA 
ATCATGCTCA GTGGTGCCGG TGCCCTCACC CCTCAGGACA TTCAGGCCCT GGCCTACGGG 
CTGTGCCCAA CCCAGCCAAA GCGAGCCCAG GAGCTCCTGA ATGAGGCAGT AGGACGGCTA 
CAGGGCCTGA CAGTACCAGG CAATAGCCAC CTTGTCTTGG TCCTAGACAA GGACTTGCAG 
AAGCTGCCGT GGGAAAGCAT GCCCAGCCTC CAGGCACTGC CTGTCACCCG GCTGCCCTCC 
TTCCGCTTCC TACTCAGCTA CTCCATCATC AAAGAGTATG GGGCCTCGCC AGTGCTGAGT 
CAAGGGGTGA ATCCGCGAAG TACCTTCTAT GTCCTGAACC CTCACAATAA CCTGTCAAGC 
ACAGAGGAGC AATTTCGAGC CAATTTCAGC AGTGAAGCTG GCTGGAGAGG AGTGGTTGGG 
GAGGTGCCAA GACCTGAACA GGTGCAAGAA GCCCTGACAA AGCATGATTT GTATATCTAT 
GCAGGGCATG GGGCTGGTGC CCGCTTCCTT GACGGGCAGG CTGTCCTGCG GCTGAGCTGT 
CGGGCAGTGG CCCTGCTGTT TGGCTGTAGC AGTGCGGCCC TGGCTGTGCA TGGAAACCTG 
GAGGGGGCTG GCATTGTGCT CAAGTACATC ATGGCTGGTT GCCCCTTGTT TCTGGGTAAT 
CTCTGGGATG TGACTGACCG TGACATTGAC CGCTACACGG AAGCTCTGCT GCAGGGCTGG 
CTTGGAGCGG GCCCAGGGGC CCCCCTTCTC TACTATGTAA ACCAGGCCCG CCAAGCTCCC 
CGACTCAAGT ATCTTATTGG GGCTGCACCT ATAGCCTATG GCTTGCCTGT CTCTCTGCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_024257504.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024113272.1 Pongo abelii extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.

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CloneID	OSa168214
Clone ID Related Accession (Same CDS sequence)	XM_024257504.1 , XM_024257505.1 , XM_024257506.1 , XM_024257507.1
Accession Version	XM_024257504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	separin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCGGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTGGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC AATCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAGA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTACAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT ACGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATATA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGAGAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACTACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCTGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAACCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA AGAGCTGCAG GCTTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCTGGGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GAGTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGCAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTACTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGCGCCTGA AGCATCTCAA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACTGAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAGCCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CGCCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAGG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGCG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA GGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCCGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAGCCA GCCATCAAAC 
AAGAGCCTGC AGAAGGTTCT AGAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAGCT AGCTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCGGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGGTAG CCTCTGCTCC CCTGAGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTTC CCTGCACACC TAAACCCCCA GGCCGAATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAAAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGGCTAAGAG ACGGGGCACT 
GCTTCCCGGG GCCGGGGGCG AGCAAGGAAG GGTATGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAGTGGCA GGAGCTGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCAGCGT CCCCGGAGGG CCAATGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAGGAACTGA CTGACAACTG GAGAGAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGATGGG GAAGACTCAG CCTCAGGTGG GAAGGCTCCA 
GCGCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAACTGC CCAGCCCATG CCCAGACAAG GACAGTGACA AGGACCTTGG TCCTCGGCTC 
CAGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC GTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGACCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GAGGCTGAGC 
CTTCAGGAGA CGCCTAGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGGAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCCTCTG CGTTCAGTCC TGAATGAATT CGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACGGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGAATGTGGC TGGAAATATC CTGACCCCAC TCTGCTGAAA 
ATCATGCTCA GTGGTGCCGG TGCCCTCACC CCTCAGGACA TTCAGGCCCT GGCCTACGGG 
CTGTGCCCAA CCCAGCCAAA GCGAGCCCAG GAGCTCCTGA ATGAGGCAGT AGGACGGCTA 
CAGGGCCTGA CAGTACCAGG CAATAGCCAC CTTGTCTTGG TCCTAGACAA GGACTTGCAG 
AAGCTGCCGT GGGAAAGCAT GCCCAGCCTC CAGGCACTGC CTGTCACCCG GCTGCCCTCC 
TTCCGCTTCC TACTCAGCTA CTCCATCATC AAAGAGTATG GGGCCTCGCC AGTGCTGAGT 
CAAGGGGTGA ATCCGCGAAG TACCTTCTAT GTCCTGAACC CTCACAATAA CCTGTCAAGC 
ACAGAGGAGC AATTTCGAGC CAATTTCAGC AGTGAAGCTG GCTGGAGAGG AGTGGTTGGG 
GAGGTGCCAA GACCTGAACA GGTGCAAGAA GCCCTGACAA AGCATGATTT GTATATCTAT 
GCAGGGCATG GGGCTGGTGC CCGCTTCCTT GACGGGCAGG CTGTCCTGCG GCTGAGCTGT 
CGGGCAGTGG CCCTGCTGTT TGGCTGTAGC AGTGCGGCCC TGGCTGTGCA TGGAAACCTG 
GAGGGGGCTG GCATTGTGCT CAAGTACATC ATGGCTGGTT GCCCCTTGTT TCTGGGTAAT 
CTCTGGGATG TGACTGACCG TGACATTGAC CGCTACACGG AAGCTCTGCT GCAGGGCTGG 
CTTGGAGCGG GCCCAGGGGC CCCCCTTCTC TACTATGTAA ACCAGGCCCG CCAAGCTCCC 
CGACTCAAGT ATCTTATTGG GGCTGCACCT ATAGCCTATG GCTTGCCTGT CTCTCTGCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024113272.1
CDS	138..6500
Translation	MRSFKRVNFGTLLSSRKEAEELLPDLKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATIRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRVVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDRAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQIYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVESCKSTVVWMLEALEGLSGQELTDYMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDNLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPGETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLESVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRAQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASRLKHLNQTTDTYLLLSLTCDLLRSQLYWTHQKVTEGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNSLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLSQPSNKSLQKVLESGLKFVAARIPHLEPWRASLLLIWALTKLAGLSCCTTQLFASSWGWRPPLIKSVPGSEPSKTQGQKRSGRGRQKVASAPLSLNNTSQKGLEGRGLPCTPKPPGRIRQAGPHVPFTVFEEVCPTKSKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEAKRRGTASRGRGRARKGMSLKTDAVVAPGSAPGNPGLSGRSWRAKKVASRHCEEQRPRRANDQARPGPEIMRTIPEEELTDNWREMSFEILRGSDGEDSASGGKAPAPGPEAASGEWELLRLDSSKKELPSPCPDKDSDKDLGPRLQLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKTQKHRGSLEIADQLQRLSLQETPRDVPLARIQRLFSFRALGSGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLPLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQECGWKYPDPTLLKIMLSGAGALTPQDIQALAYGLCPTQPKRAQELLNEAVGRLQGLTVPGNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVNPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR

Target ORF information:

RefSeq Version	XM_024257504.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii extra spindle pole bodies like 1, separase (ESPL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024257504.1

ORF Insert Sequence:

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCGGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTGGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC AATCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAGA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTACAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT ACGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATATA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGAGAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACTACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCTGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAACCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA AGAGCTGCAG GCTTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCTGGGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GAGTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGCAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTACTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGCGCCTGA AGCATCTCAA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACTGAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAGCCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CGCCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAGG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGCG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA GGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCCGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAGCCA GCCATCAAAC 
AAGAGCCTGC AGAAGGTTCT AGAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAGCT AGCTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCGGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGGTAG CCTCTGCTCC CCTGAGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTTC CCTGCACACC TAAACCCCCA GGCCGAATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAAAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGGCTAAGAG ACGGGGCACT 
GCTTCCCGGG GCCGGGGGCG AGCAAGGAAG GGTATGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAGTGGCA GGAGCTGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCAGCGT CCCCGGAGGG CCAATGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAGGAACTGA CTGACAACTG GAGAGAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGATGGG GAAGACTCAG CCTCAGGTGG GAAGGCTCCA 
GCGCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAACTGC CCAGCCCATG CCCAGACAAG GACAGTGACA AGGACCTTGG TCCTCGGCTC 
CAGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC GTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGACCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GAGGCTGAGC 
CTTCAGGAGA CGCCTAGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGGAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCCTCTG CGTTCAGTCC TGAATGAATT CGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACGGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGAATGTGGC TGGAAATATC CTGACCCCAC TCTGCTGAAA 
ATCATGCTCA GTGGTGCCGG TGCCCTCACC CCTCAGGACA TTCAGGCCCT GGCCTACGGG 
CTGTGCCCAA CCCAGCCAAA GCGAGCCCAG GAGCTCCTGA ATGAGGCAGT AGGACGGCTA 
CAGGGCCTGA CAGTACCAGG CAATAGCCAC CTTGTCTTGG TCCTAGACAA GGACTTGCAG 
AAGCTGCCGT GGGAAAGCAT GCCCAGCCTC CAGGCACTGC CTGTCACCCG GCTGCCCTCC 
TTCCGCTTCC TACTCAGCTA CTCCATCATC AAAGAGTATG GGGCCTCGCC AGTGCTGAGT 
CAAGGGGTGA ATCCGCGAAG TACCTTCTAT GTCCTGAACC CTCACAATAA CCTGTCAAGC 
ACAGAGGAGC AATTTCGAGC CAATTTCAGC AGTGAAGCTG GCTGGAGAGG AGTGGTTGGG 
GAGGTGCCAA GACCTGAACA GGTGCAAGAA GCCCTGACAA AGCATGATTT GTATATCTAT 
GCAGGGCATG GGGCTGGTGC CCGCTTCCTT GACGGGCAGG CTGTCCTGCG GCTGAGCTGT 
CGGGCAGTGG CCCTGCTGTT TGGCTGTAGC AGTGCGGCCC TGGCTGTGCA TGGAAACCTG 
GAGGGGGCTG GCATTGTGCT CAAGTACATC ATGGCTGGTT GCCCCTTGTT TCTGGGTAAT 
CTCTGGGATG TGACTGACCG TGACATTGAC CGCTACACGG AAGCTCTGCT GCAGGGCTGG 
CTTGGAGCGG GCCCAGGGGC CCCCCTTCTC TACTATGTAA ACCAGGCCCG CCAAGCTCCC 
CGACTCAAGT ATCTTATTGG GGCTGCACCT ATAGCCTATG GCTTGCCTGT CTCTCTGCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_024257505.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024113273.1 Pongo abelii extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.

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CloneID	OSa168214
Clone ID Related Accession (Same CDS sequence)	XM_024257504.1 , XM_024257505.1 , XM_024257506.1 , XM_024257507.1
Accession Version	XM_024257505.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	separin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCGGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTGGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC AATCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAGA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTACAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT ACGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATATA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGAGAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACTACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCTGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAACCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA AGAGCTGCAG GCTTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCTGGGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GAGTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGCAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTACTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGCGCCTGA AGCATCTCAA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACTGAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAGCCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CGCCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAGG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGCG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA GGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCCGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAGCCA GCCATCAAAC 
AAGAGCCTGC AGAAGGTTCT AGAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAGCT AGCTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCGGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGGTAG CCTCTGCTCC CCTGAGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTTC CCTGCACACC TAAACCCCCA GGCCGAATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAAAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGGCTAAGAG ACGGGGCACT 
GCTTCCCGGG GCCGGGGGCG AGCAAGGAAG GGTATGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAGTGGCA GGAGCTGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCAGCGT CCCCGGAGGG CCAATGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAGGAACTGA CTGACAACTG GAGAGAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGATGGG GAAGACTCAG CCTCAGGTGG GAAGGCTCCA 
GCGCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAACTGC CCAGCCCATG CCCAGACAAG GACAGTGACA AGGACCTTGG TCCTCGGCTC 
CAGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC GTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGACCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GAGGCTGAGC 
CTTCAGGAGA CGCCTAGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGGAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCCTCTG CGTTCAGTCC TGAATGAATT CGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACGGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGAATGTGGC TGGAAATATC CTGACCCCAC TCTGCTGAAA 
ATCATGCTCA GTGGTGCCGG TGCCCTCACC CCTCAGGACA TTCAGGCCCT GGCCTACGGG 
CTGTGCCCAA CCCAGCCAAA GCGAGCCCAG GAGCTCCTGA ATGAGGCAGT AGGACGGCTA 
CAGGGCCTGA CAGTACCAGG CAATAGCCAC CTTGTCTTGG TCCTAGACAA GGACTTGCAG 
AAGCTGCCGT GGGAAAGCAT GCCCAGCCTC CAGGCACTGC CTGTCACCCG GCTGCCCTCC 
TTCCGCTTCC TACTCAGCTA CTCCATCATC AAAGAGTATG GGGCCTCGCC AGTGCTGAGT 
CAAGGGGTGA ATCCGCGAAG TACCTTCTAT GTCCTGAACC CTCACAATAA CCTGTCAAGC 
ACAGAGGAGC AATTTCGAGC CAATTTCAGC AGTGAAGCTG GCTGGAGAGG AGTGGTTGGG 
GAGGTGCCAA GACCTGAACA GGTGCAAGAA GCCCTGACAA AGCATGATTT GTATATCTAT 
GCAGGGCATG GGGCTGGTGC CCGCTTCCTT GACGGGCAGG CTGTCCTGCG GCTGAGCTGT 
CGGGCAGTGG CCCTGCTGTT TGGCTGTAGC AGTGCGGCCC TGGCTGTGCA TGGAAACCTG 
GAGGGGGCTG GCATTGTGCT CAAGTACATC ATGGCTGGTT GCCCCTTGTT TCTGGGTAAT 
CTCTGGGATG TGACTGACCG TGACATTGAC CGCTACACGG AAGCTCTGCT GCAGGGCTGG 
CTTGGAGCGG GCCCAGGGGC CCCCCTTCTC TACTATGTAA ACCAGGCCCG CCAAGCTCCC 
CGACTCAAGT ATCTTATTGG GGCTGCACCT ATAGCCTATG GCTTGCCTGT CTCTCTGCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024113273.1
CDS	134..6496
Translation	MRSFKRVNFGTLLSSRKEAEELLPDLKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATIRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRVVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDRAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQIYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVESCKSTVVWMLEALEGLSGQELTDYMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDNLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPGETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLESVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRAQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASRLKHLNQTTDTYLLLSLTCDLLRSQLYWTHQKVTEGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNSLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLSQPSNKSLQKVLESGLKFVAARIPHLEPWRASLLLIWALTKLAGLSCCTTQLFASSWGWRPPLIKSVPGSEPSKTQGQKRSGRGRQKVASAPLSLNNTSQKGLEGRGLPCTPKPPGRIRQAGPHVPFTVFEEVCPTKSKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEAKRRGTASRGRGRARKGMSLKTDAVVAPGSAPGNPGLSGRSWRAKKVASRHCEEQRPRRANDQARPGPEIMRTIPEEELTDNWREMSFEILRGSDGEDSASGGKAPAPGPEAASGEWELLRLDSSKKELPSPCPDKDSDKDLGPRLQLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKTQKHRGSLEIADQLQRLSLQETPRDVPLARIQRLFSFRALGSGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLPLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQECGWKYPDPTLLKIMLSGAGALTPQDIQALAYGLCPTQPKRAQELLNEAVGRLQGLTVPGNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVNPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR

Target ORF information:

RefSeq Version	XM_024257505.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii extra spindle pole bodies like 1, separase (ESPL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024257505.1

ORF Insert Sequence:

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCGGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTGGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC AATCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAGA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTACAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT ACGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATATA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGAGAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACTACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCTGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAACCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA AGAGCTGCAG GCTTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCTGGGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GAGTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGCAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTACTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGCGCCTGA AGCATCTCAA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACTGAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAGCCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CGCCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAGG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGCG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA GGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCCGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAGCCA GCCATCAAAC 
AAGAGCCTGC AGAAGGTTCT AGAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAGCT AGCTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCGGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGGTAG CCTCTGCTCC CCTGAGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTTC CCTGCACACC TAAACCCCCA GGCCGAATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAAAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGGCTAAGAG ACGGGGCACT 
GCTTCCCGGG GCCGGGGGCG AGCAAGGAAG GGTATGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAGTGGCA GGAGCTGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCAGCGT CCCCGGAGGG CCAATGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAGGAACTGA CTGACAACTG GAGAGAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGATGGG GAAGACTCAG CCTCAGGTGG GAAGGCTCCA 
GCGCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAACTGC CCAGCCCATG CCCAGACAAG GACAGTGACA AGGACCTTGG TCCTCGGCTC 
CAGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC GTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGACCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GAGGCTGAGC 
CTTCAGGAGA CGCCTAGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGGAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCCTCTG CGTTCAGTCC TGAATGAATT CGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACGGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGAATGTGGC TGGAAATATC CTGACCCCAC TCTGCTGAAA 
ATCATGCTCA GTGGTGCCGG TGCCCTCACC CCTCAGGACA TTCAGGCCCT GGCCTACGGG 
CTGTGCCCAA CCCAGCCAAA GCGAGCCCAG GAGCTCCTGA ATGAGGCAGT AGGACGGCTA 
CAGGGCCTGA CAGTACCAGG CAATAGCCAC CTTGTCTTGG TCCTAGACAA GGACTTGCAG 
AAGCTGCCGT GGGAAAGCAT GCCCAGCCTC CAGGCACTGC CTGTCACCCG GCTGCCCTCC 
TTCCGCTTCC TACTCAGCTA CTCCATCATC AAAGAGTATG GGGCCTCGCC AGTGCTGAGT 
CAAGGGGTGA ATCCGCGAAG TACCTTCTAT GTCCTGAACC CTCACAATAA CCTGTCAAGC 
ACAGAGGAGC AATTTCGAGC CAATTTCAGC AGTGAAGCTG GCTGGAGAGG AGTGGTTGGG 
GAGGTGCCAA GACCTGAACA GGTGCAAGAA GCCCTGACAA AGCATGATTT GTATATCTAT 
GCAGGGCATG GGGCTGGTGC CCGCTTCCTT GACGGGCAGG CTGTCCTGCG GCTGAGCTGT 
CGGGCAGTGG CCCTGCTGTT TGGCTGTAGC AGTGCGGCCC TGGCTGTGCA TGGAAACCTG 
GAGGGGGCTG GCATTGTGCT CAAGTACATC ATGGCTGGTT GCCCCTTGTT TCTGGGTAAT 
CTCTGGGATG TGACTGACCG TGACATTGAC CGCTACACGG AAGCTCTGCT GCAGGGCTGG 
CTTGGAGCGG GCCCAGGGGC CCCCCTTCTC TACTATGTAA ACCAGGCCCG CCAAGCTCCC 
CGACTCAAGT ATCTTATTGG GGCTGCACCT ATAGCCTATG GCTTGCCTGT CTCTCTGCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_024257506.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024113274.1 Pongo abelii extra spindle pole bodies like 1, separase (ESPL1), transcript variant X3, mRNA.

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CloneID	OSa168214
Clone ID Related Accession (Same CDS sequence)	XM_024257504.1 , XM_024257505.1 , XM_024257506.1 , XM_024257507.1
Accession Version	XM_024257506.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	separin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCGGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTGGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC AATCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAGA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTACAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT ACGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATATA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGAGAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACTACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCTGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAACCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA AGAGCTGCAG GCTTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCTGGGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GAGTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGCAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTACTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGCGCCTGA AGCATCTCAA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACTGAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAGCCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CGCCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAGG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGCG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA GGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCCGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAGCCA GCCATCAAAC 
AAGAGCCTGC AGAAGGTTCT AGAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAGCT AGCTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCGGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGGTAG CCTCTGCTCC CCTGAGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTTC CCTGCACACC TAAACCCCCA GGCCGAATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAAAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGGCTAAGAG ACGGGGCACT 
GCTTCCCGGG GCCGGGGGCG AGCAAGGAAG GGTATGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAGTGGCA GGAGCTGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCAGCGT CCCCGGAGGG CCAATGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAGGAACTGA CTGACAACTG GAGAGAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGATGGG GAAGACTCAG CCTCAGGTGG GAAGGCTCCA 
GCGCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAACTGC CCAGCCCATG CCCAGACAAG GACAGTGACA AGGACCTTGG TCCTCGGCTC 
CAGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC GTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGACCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GAGGCTGAGC 
CTTCAGGAGA CGCCTAGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGGAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCCTCTG CGTTCAGTCC TGAATGAATT CGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACGGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGAATGTGGC TGGAAATATC CTGACCCCAC TCTGCTGAAA 
ATCATGCTCA GTGGTGCCGG TGCCCTCACC CCTCAGGACA TTCAGGCCCT GGCCTACGGG 
CTGTGCCCAA CCCAGCCAAA GCGAGCCCAG GAGCTCCTGA ATGAGGCAGT AGGACGGCTA 
CAGGGCCTGA CAGTACCAGG CAATAGCCAC CTTGTCTTGG TCCTAGACAA GGACTTGCAG 
AAGCTGCCGT GGGAAAGCAT GCCCAGCCTC CAGGCACTGC CTGTCACCCG GCTGCCCTCC 
TTCCGCTTCC TACTCAGCTA CTCCATCATC AAAGAGTATG GGGCCTCGCC AGTGCTGAGT 
CAAGGGGTGA ATCCGCGAAG TACCTTCTAT GTCCTGAACC CTCACAATAA CCTGTCAAGC 
ACAGAGGAGC AATTTCGAGC CAATTTCAGC AGTGAAGCTG GCTGGAGAGG AGTGGTTGGG 
GAGGTGCCAA GACCTGAACA GGTGCAAGAA GCCCTGACAA AGCATGATTT GTATATCTAT 
GCAGGGCATG GGGCTGGTGC CCGCTTCCTT GACGGGCAGG CTGTCCTGCG GCTGAGCTGT 
CGGGCAGTGG CCCTGCTGTT TGGCTGTAGC AGTGCGGCCC TGGCTGTGCA TGGAAACCTG 
GAGGGGGCTG GCATTGTGCT CAAGTACATC ATGGCTGGTT GCCCCTTGTT TCTGGGTAAT 
CTCTGGGATG TGACTGACCG TGACATTGAC CGCTACACGG AAGCTCTGCT GCAGGGCTGG 
CTTGGAGCGG GCCCAGGGGC CCCCCTTCTC TACTATGTAA ACCAGGCCCG CCAAGCTCCC 
CGACTCAAGT ATCTTATTGG GGCTGCACCT ATAGCCTATG GCTTGCCTGT CTCTCTGCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024113274.1
CDS	361..6723
Translation	MRSFKRVNFGTLLSSRKEAEELLPDLKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATIRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRVVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDRAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQIYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVESCKSTVVWMLEALEGLSGQELTDYMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDNLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPGETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLESVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRAQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASRLKHLNQTTDTYLLLSLTCDLLRSQLYWTHQKVTEGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNSLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLSQPSNKSLQKVLESGLKFVAARIPHLEPWRASLLLIWALTKLAGLSCCTTQLFASSWGWRPPLIKSVPGSEPSKTQGQKRSGRGRQKVASAPLSLNNTSQKGLEGRGLPCTPKPPGRIRQAGPHVPFTVFEEVCPTKSKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEAKRRGTASRGRGRARKGMSLKTDAVVAPGSAPGNPGLSGRSWRAKKVASRHCEEQRPRRANDQARPGPEIMRTIPEEELTDNWREMSFEILRGSDGEDSASGGKAPAPGPEAASGEWELLRLDSSKKELPSPCPDKDSDKDLGPRLQLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKTQKHRGSLEIADQLQRLSLQETPRDVPLARIQRLFSFRALGSGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLPLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQECGWKYPDPTLLKIMLSGAGALTPQDIQALAYGLCPTQPKRAQELLNEAVGRLQGLTVPGNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVNPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR

Target ORF information:

RefSeq Version	XM_024257506.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii extra spindle pole bodies like 1, separase (ESPL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024257506.1

ORF Insert Sequence:

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCGGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTGGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC AATCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAGA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTACAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT ACGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATATA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGAGAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACTACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCTGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAACCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA AGAGCTGCAG GCTTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCTGGGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GAGTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGCAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTACTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGCGCCTGA AGCATCTCAA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACTGAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAGCCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CGCCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAGG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGCG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA GGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCCGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAGCCA GCCATCAAAC 
AAGAGCCTGC AGAAGGTTCT AGAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAGCT AGCTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCGGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGGTAG CCTCTGCTCC CCTGAGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTTC CCTGCACACC TAAACCCCCA GGCCGAATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAAAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGGCTAAGAG ACGGGGCACT 
GCTTCCCGGG GCCGGGGGCG AGCAAGGAAG GGTATGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAGTGGCA GGAGCTGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCAGCGT CCCCGGAGGG CCAATGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAGGAACTGA CTGACAACTG GAGAGAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGATGGG GAAGACTCAG CCTCAGGTGG GAAGGCTCCA 
GCGCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAACTGC CCAGCCCATG CCCAGACAAG GACAGTGACA AGGACCTTGG TCCTCGGCTC 
CAGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC GTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGACCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GAGGCTGAGC 
CTTCAGGAGA CGCCTAGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGGAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCCTCTG CGTTCAGTCC TGAATGAATT CGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACGGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGAATGTGGC TGGAAATATC CTGACCCCAC TCTGCTGAAA 
ATCATGCTCA GTGGTGCCGG TGCCCTCACC CCTCAGGACA TTCAGGCCCT GGCCTACGGG 
CTGTGCCCAA CCCAGCCAAA GCGAGCCCAG GAGCTCCTGA ATGAGGCAGT AGGACGGCTA 
CAGGGCCTGA CAGTACCAGG CAATAGCCAC CTTGTCTTGG TCCTAGACAA GGACTTGCAG 
AAGCTGCCGT GGGAAAGCAT GCCCAGCCTC CAGGCACTGC CTGTCACCCG GCTGCCCTCC 
TTCCGCTTCC TACTCAGCTA CTCCATCATC AAAGAGTATG GGGCCTCGCC AGTGCTGAGT 
CAAGGGGTGA ATCCGCGAAG TACCTTCTAT GTCCTGAACC CTCACAATAA CCTGTCAAGC 
ACAGAGGAGC AATTTCGAGC CAATTTCAGC AGTGAAGCTG GCTGGAGAGG AGTGGTTGGG 
GAGGTGCCAA GACCTGAACA GGTGCAAGAA GCCCTGACAA AGCATGATTT GTATATCTAT 
GCAGGGCATG GGGCTGGTGC CCGCTTCCTT GACGGGCAGG CTGTCCTGCG GCTGAGCTGT 
CGGGCAGTGG CCCTGCTGTT TGGCTGTAGC AGTGCGGCCC TGGCTGTGCA TGGAAACCTG 
GAGGGGGCTG GCATTGTGCT CAAGTACATC ATGGCTGGTT GCCCCTTGTT TCTGGGTAAT 
CTCTGGGATG TGACTGACCG TGACATTGAC CGCTACACGG AAGCTCTGCT GCAGGGCTGG 
CTTGGAGCGG GCCCAGGGGC CCCCCTTCTC TACTATGTAA ACCAGGCCCG CCAAGCTCCC 
CGACTCAAGT ATCTTATTGG GGCTGCACCT ATAGCCTATG GCTTGCCTGT CTCTCTGCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_024257507.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024113275.1 Pongo abelii extra spindle pole bodies like 1, separase (ESPL1), transcript variant X4, mRNA.

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CloneID	OSa168214
Clone ID Related Accession (Same CDS sequence)	XM_024257504.1 , XM_024257505.1 , XM_024257506.1 , XM_024257507.1
Accession Version	XM_024257507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	separin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCGGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTGGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC AATCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAGA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTACAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT ACGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATATA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGAGAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACTACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCTGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAACCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA AGAGCTGCAG GCTTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCTGGGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GAGTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGCAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTACTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGCGCCTGA AGCATCTCAA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACTGAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAGCCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CGCCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAGG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGCG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA GGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCCGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAGCCA GCCATCAAAC 
AAGAGCCTGC AGAAGGTTCT AGAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAGCT AGCTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCGGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGGTAG CCTCTGCTCC CCTGAGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTTC CCTGCACACC TAAACCCCCA GGCCGAATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAAAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGGCTAAGAG ACGGGGCACT 
GCTTCCCGGG GCCGGGGGCG AGCAAGGAAG GGTATGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAGTGGCA GGAGCTGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCAGCGT CCCCGGAGGG CCAATGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAGGAACTGA CTGACAACTG GAGAGAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGATGGG GAAGACTCAG CCTCAGGTGG GAAGGCTCCA 
GCGCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAACTGC CCAGCCCATG CCCAGACAAG GACAGTGACA AGGACCTTGG TCCTCGGCTC 
CAGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC GTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGACCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GAGGCTGAGC 
CTTCAGGAGA CGCCTAGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGGAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCCTCTG CGTTCAGTCC TGAATGAATT CGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACGGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGAATGTGGC TGGAAATATC CTGACCCCAC TCTGCTGAAA 
ATCATGCTCA GTGGTGCCGG TGCCCTCACC CCTCAGGACA TTCAGGCCCT GGCCTACGGG 
CTGTGCCCAA CCCAGCCAAA GCGAGCCCAG GAGCTCCTGA ATGAGGCAGT AGGACGGCTA 
CAGGGCCTGA CAGTACCAGG CAATAGCCAC CTTGTCTTGG TCCTAGACAA GGACTTGCAG 
AAGCTGCCGT GGGAAAGCAT GCCCAGCCTC CAGGCACTGC CTGTCACCCG GCTGCCCTCC 
TTCCGCTTCC TACTCAGCTA CTCCATCATC AAAGAGTATG GGGCCTCGCC AGTGCTGAGT 
CAAGGGGTGA ATCCGCGAAG TACCTTCTAT GTCCTGAACC CTCACAATAA CCTGTCAAGC 
ACAGAGGAGC AATTTCGAGC CAATTTCAGC AGTGAAGCTG GCTGGAGAGG AGTGGTTGGG 
GAGGTGCCAA GACCTGAACA GGTGCAAGAA GCCCTGACAA AGCATGATTT GTATATCTAT 
GCAGGGCATG GGGCTGGTGC CCGCTTCCTT GACGGGCAGG CTGTCCTGCG GCTGAGCTGT 
CGGGCAGTGG CCCTGCTGTT TGGCTGTAGC AGTGCGGCCC TGGCTGTGCA TGGAAACCTG 
GAGGGGGCTG GCATTGTGCT CAAGTACATC ATGGCTGGTT GCCCCTTGTT TCTGGGTAAT 
CTCTGGGATG TGACTGACCG TGACATTGAC CGCTACACGG AAGCTCTGCT GCAGGGCTGG 
CTTGGAGCGG GCCCAGGGGC CCCCCTTCTC TACTATGTAA ACCAGGCCCG CCAAGCTCCC 
CGACTCAAGT ATCTTATTGG GGCTGCACCT ATAGCCTATG GCTTGCCTGT CTCTCTGCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024113275.1
CDS	109..6471
Translation	MRSFKRVNFGTLLSSRKEAEELLPDLKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATIRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRVVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDRAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQIYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVESCKSTVVWMLEALEGLSGQELTDYMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDNLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPGETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLESVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRAQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASRLKHLNQTTDTYLLLSLTCDLLRSQLYWTHQKVTEGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNSLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLSQPSNKSLQKVLESGLKFVAARIPHLEPWRASLLLIWALTKLAGLSCCTTQLFASSWGWRPPLIKSVPGSEPSKTQGQKRSGRGRQKVASAPLSLNNTSQKGLEGRGLPCTPKPPGRIRQAGPHVPFTVFEEVCPTKSKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEAKRRGTASRGRGRARKGMSLKTDAVVAPGSAPGNPGLSGRSWRAKKVASRHCEEQRPRRANDQARPGPEIMRTIPEEELTDNWREMSFEILRGSDGEDSASGGKAPAPGPEAASGEWELLRLDSSKKELPSPCPDKDSDKDLGPRLQLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKTQKHRGSLEIADQLQRLSLQETPRDVPLARIQRLFSFRALGSGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLPLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQECGWKYPDPTLLKIMLSGAGALTPQDIQALAYGLCPTQPKRAQELLNEAVGRLQGLTVPGNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVNPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR

Target ORF information:

RefSeq Version	XM_024257507.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii extra spindle pole bodies like 1, separase (ESPL1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024257507.1

ORF Insert Sequence:

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCGGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTGGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC AATCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAGA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTACAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT ACGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATATA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGAGAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACTACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCTGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAACCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA AGAGCTGCAG GCTTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCTGGGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GAGTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGCAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTACTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGCGCCTGA AGCATCTCAA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACTGAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAGCCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CGCCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAGG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGCG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA GGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCCGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAGCCA GCCATCAAAC 
AAGAGCCTGC AGAAGGTTCT AGAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAGCT AGCTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCGGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGGTAG CCTCTGCTCC CCTGAGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTTC CCTGCACACC TAAACCCCCA GGCCGAATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAAAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGGCTAAGAG ACGGGGCACT 
GCTTCCCGGG GCCGGGGGCG AGCAAGGAAG GGTATGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAGTGGCA GGAGCTGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCAGCGT CCCCGGAGGG CCAATGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAGGAACTGA CTGACAACTG GAGAGAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGATGGG GAAGACTCAG CCTCAGGTGG GAAGGCTCCA 
GCGCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAACTGC CCAGCCCATG CCCAGACAAG GACAGTGACA AGGACCTTGG TCCTCGGCTC 
CAGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC GTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGACCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GAGGCTGAGC 
CTTCAGGAGA CGCCTAGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGGAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCCTCTG CGTTCAGTCC TGAATGAATT CGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACGGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGAATGTGGC TGGAAATATC CTGACCCCAC TCTGCTGAAA 
ATCATGCTCA GTGGTGCCGG TGCCCTCACC CCTCAGGACA TTCAGGCCCT GGCCTACGGG 
CTGTGCCCAA CCCAGCCAAA GCGAGCCCAG GAGCTCCTGA ATGAGGCAGT AGGACGGCTA 
CAGGGCCTGA CAGTACCAGG CAATAGCCAC CTTGTCTTGG TCCTAGACAA GGACTTGCAG 
AAGCTGCCGT GGGAAAGCAT GCCCAGCCTC CAGGCACTGC CTGTCACCCG GCTGCCCTCC 
TTCCGCTTCC TACTCAGCTA CTCCATCATC AAAGAGTATG GGGCCTCGCC AGTGCTGAGT 
CAAGGGGTGA ATCCGCGAAG TACCTTCTAT GTCCTGAACC CTCACAATAA CCTGTCAAGC 
ACAGAGGAGC AATTTCGAGC CAATTTCAGC AGTGAAGCTG GCTGGAGAGG AGTGGTTGGG 
GAGGTGCCAA GACCTGAACA GGTGCAAGAA GCCCTGACAA AGCATGATTT GTATATCTAT 
GCAGGGCATG GGGCTGGTGC CCGCTTCCTT GACGGGCAGG CTGTCCTGCG GCTGAGCTGT 
CGGGCAGTGG CCCTGCTGTT TGGCTGTAGC AGTGCGGCCC TGGCTGTGCA TGGAAACCTG 
GAGGGGGCTG GCATTGTGCT CAAGTACATC ATGGCTGGTT GCCCCTTGTT TCTGGGTAAT 
CTCTGGGATG TGACTGACCG TGACATTGAC CGCTACACGG AAGCTCTGCT GCAGGGCTGG 
CTTGGAGCGG GCCCAGGGGC CCCCCTTCTC TACTATGTAA ACCAGGCCCG CCAAGCTCCC 
CGACTCAAGT ATCTTATTGG GGCTGCACCT ATAGCCTATG GCTTGCCTGT CTCTCTGCGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_024257508.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024113276.1 Pongo abelii extra spindle pole bodies like 1, separase (ESPL1), transcript variant X5, mRNA.

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CloneID	OSa168215
Clone ID Related Accession (Same CDS sequence)	XM_024257508.1
Accession Version	XM_024257508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6087bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	separin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCGGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTGGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC AATCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAGA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTACAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT ACGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATATA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGAGAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACTACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCTGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAACCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA AGAGCTGCAG GCTTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCTGGGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GAGTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGCAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTACTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGCGCCTGA AGCATCTCAA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACTGAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAGCCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CGCCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAGG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGCG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA GGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCCGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAGCCA GCCATCAAAC 
AAGAGCCTGC AGAAGGTTCT AGAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAGCT AGCTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCGGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGGTAG CCTCTGCTCC CCTGAGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTTC CCTGCACACC TAAACCCCCA GGCCGAATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAAAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGGCTAAGAG ACGGGGCACT 
GCTTCCCGGG GCCGGGGGCG AGCAAGGAAG GGTATGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAGTGGCA GGAGCTGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCAGCGT CCCCGGAGGG CCAATGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAGGAACTGA CTGACAACTG GAGAGAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGATGGG GAAGACTCAG CCTCAGGTGG GAAGGCTCCA 
GCGCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAACTGC CCAGCCCATG CCCAGACAAG GACAGTGACA AGGACCTTGG TCCTCGGCTC 
CAGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC GTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGACCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GAGGCTGAGC 
CTTCAGGAGA CGCCTAGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGGAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCCTCTG CGTTCAGTCC TGAATGAATT CGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACGGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGAATGTGGC TGGAAATATC CTGACCCCAC TCTGCTGAAA 
TATGGGGCCT CGCCAGTGCT GAGTCAAGGG GTGAATCCGC GAAGTACCTT CTATGTCCTG 
AACCCTCACA ATAACCTGTC AAGCACAGAG GAGCAATTTC GAGCCAATTT CAGCAGTGAA 
GCTGGCTGGA GAGGAGTGGT TGGGGAGGTG CCAAGACCTG AACAGGTGCA AGAAGCCCTG 
ACAAAGCATG ATTTGTATAT CTATGCAGGG CATGGGGCTG GTGCCCGCTT CCTTGACGGG 
CAGGCTGTCC TGCGGCTGAG CTGTCGGGCA GTGGCCCTGC TGTTTGGCTG TAGCAGTGCG 
GCCCTGGCTG TGCATGGAAA CCTGGAGGGG GCTGGCATTG TGCTCAAGTA CATCATGGCT 
GGTTGCCCCT TGTTTCTGGG TAATCTCTGG GATGTGACTG ACCGTGACAT TGACCGCTAC 
ACGGAAGCTC TGCTGCAGGG CTGGCTTGGA GCGGGCCCAG GGGCCCCCCT TCTCTACTAT 
GTAAACCAGG CCCGCCAAGC TCCCCGACTC AAGTATCTTA TTGGGGCTGC ACCTATAGCC 
TATGGCTTGC CTGTCTCTCT GCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024113276.1
CDS	138..6224
Translation	MRSFKRVNFGTLLSSRKEAEELLPDLKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATIRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRVVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDRAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQIYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVESCKSTVVWMLEALEGLSGQELTDYMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDNLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPGETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLESVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRAQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKPMQALEVLLLLRIVSERLKDHSKAAGSSCHITQLLLTLGCPSYAQLHLEEAASRLKHLNQTTDTYLLLSLTCDLLRSQLYWTHQKVTEGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNSLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLSQPSNKSLQKVLESGLKFVAARIPHLEPWRASLLLIWALTKLAGLSCCTTQLFASSWGWRPPLIKSVPGSEPSKTQGQKRSGRGRQKVASAPLSLNNTSQKGLEGRGLPCTPKPPGRIRQAGPHVPFTVFEEVCPTKSKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEAKRRGTASRGRGRARKGMSLKTDAVVAPGSAPGNPGLSGRSWRAKKVASRHCEEQRPRRANDQARPGPEIMRTIPEEELTDNWREMSFEILRGSDGEDSASGGKAPAPGPEAASGEWELLRLDSSKKELPSPCPDKDSDKDLGPRLQLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKTQKHRGSLEIADQLQRLSLQETPRDVPLARIQRLFSFRALGSGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLPLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQECGWKYPDPTLLKYGASPVLSQGVNPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR

Target ORF information:

RefSeq Version	XM_024257508.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii extra spindle pole bodies like 1, separase (ESPL1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024257508.1

ORF Insert Sequence:

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCGGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTGGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC AATCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAGA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTACAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT ACGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATATA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGAGAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACTACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCTGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAACCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA AGAGCTGCAG GCTTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCTGGGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GAGTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGCAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGCCCATG CAGGCTCTGG AGGTACTCCT GCTGCTACGG 
ATTGTCTCTG AGAGACTGAA GGACCACTCG AAGGCAGCTG GCTCCTCCTG CCACATCACC 
CAGCTCCTCC TGACCCTCGG CTGTCCCAGC TATGCCCAGT TACACCTGGA AGAGGCAGCA 
TCGCGCCTGA AGCATCTCAA TCAGACCACT GACACATACC TGCTCCTTTC CCTGACCTGT 
GATCTGCTTC GAAGTCAACT CTACTGGACT CACCAGAAGG TGACTGAGGG TGTCTCTCTG 
CTGCTGTCTG TGCTTCGGGA TCCTGCCCTC CAGAAGTCCT CCAAGGCTTG GTACTTGCTG 
CGTGTCCAGG TCCTGCAGCT GGTGGCAGCT TACCTTAGCC TCCCGTCAAA CAGCCTCTCA 
CACTCCCTGT GGGAGCAGCT CTGTGCCCAA GGCTGGCAGA CGCCTGAGAT AGCTCTCATA 
GACTCCCATA AGCTCCTCCG AAGCATCATC CTCCTGCTGA TGGGCAGTGA CATTCTCTCA 
ACTCAGAAGG CAGCTGTGGA GACATCGTTT TTGGACTATG GTGAAAATCT GGTACAAAAA 
TGGCAGGTTC TTTCAGAGGT GCTGAGCTGC TCAGAGAAGC TGGTCTGCCA CCTGGGCCGC 
CTGGGTAGCG TGAGTGAAGC CAAGGCCTTT TGCTTGGAGG CCCTAAAACT TACAACAAAG 
CTGCAGATAC CACGCCAGTG TGCCCTGTTC CTGGTGCTGA AGGGCGAGCT GGAGCTGGCC 
CGCAATGACA TTGATCTCTG TCAGTCGGAC CTGCAGCAGG TTCTGTTCTT GCTTGAGTCT 
TGCACAGAGT TTGGTGGGGT GACTCAGCAC CTGGACTCTG TGAAGAAGGT CCACCTGCAG 
AAGGGGAAGC AGCAGGCCCA GGTCCCCTGT CCTCCACAGC TCCCAGAGGA GGAGCTCTTC 
CTAAGAGGCC CTGCTCTAGA GCTGGTGGCC ACTGTGGCCA AGGAGCCTGG CCCCATAGCA 
CCTTCTACAA ACTCCTCCCC AGTCTTGAAA ACCAAGCCCC AGCCCATACC CAACTTCCTG 
TCCCATTCAC CCACCTGTGA CTGCTCGCTC TGCGCCAGCC CTGTCCTCAC AGCAGTCTGT 
CTGCGCTGGG TATTGGTCAC GGCAGGGGTG AGGCTGGCCA TGGGCCACCA GGCCCAGGGT 
CTGGATCTGC TGCAGGTCGT GCTGAAGGGC TGTCCTGAAG CCGCCGAGCG CCTCACCCAA 
GCTCTCCAAG CTTCCCTGAA TCATAAAACA CCCCCCTCCT TGGTTCCAAG CCTCTTGGAT 
GAGATCTTGG CTCAAGCATA CACACTGTTG GCACTGGAGG GCCTGAGCCA GCCATCAAAC 
AAGAGCCTGC AGAAGGTTCT AGAGTCAGGG CTGAAGTTTG TAGCAGCACG GATACCCCAC 
CTAGAGCCCT GGCGAGCCAG CCTGCTCTTG ATTTGGGCCC TCACAAAGCT AGCTGGCCTC 
AGCTGCTGTA CTACCCAACT TTTTGCAAGC TCCTGGGGCT GGCGGCCACC ATTAATAAAA 
AGTGTCCCTG GCTCAGAGCC CTCTAAGACT CAGGGCCAAA AACGTTCTGG ACGAGGGCGC 
CAAAAGGTAG CCTCTGCTCC CCTGAGCCTC AATAATACCT CTCAGAAAGG TCTGGAAGGT 
AGAGGACTTC CCTGCACACC TAAACCCCCA GGCCGAATCA GGCAAGCTGG CCCTCATGTC 
CCCTTCACGG TGTTTGAGGA AGTCTGCCCT ACAAAGAGCA AGCCTGAAGT TCCCCAGGCC 
CCCAGGGTAC AACAGAGAGT CCAGACGCGC CTCAAGGTGA ACTTCAGTGA TGACAGTGAC 
TTGGAAGACC CTGTCTCAGC TGAGGCCTGG CTGGCAGAGG AGGCTAAGAG ACGGGGCACT 
GCTTCCCGGG GCCGGGGGCG AGCAAGGAAG GGTATGAGCC TAAAGACGGA TGCCGTGGTT 
GCCCCAGGTA GTGCCCCTGG GAACCCTGGC CTGAGTGGCA GGAGCTGGAG GGCCAAGAAG 
GTGGCATCAA GACATTGTGA GGAGCAGCGT CCCCGGAGGG CCAATGACCA GGCCAGGCCT 
GGCCCTGAGA TCATGAGGAC CATCCCTGAG GAGGAACTGA CTGACAACTG GAGAGAAATG 
AGCTTTGAGA TCCTCAGGGG CTCTGATGGG GAAGACTCAG CCTCAGGTGG GAAGGCTCCA 
GCGCCGGGCC CTGAGGCAGC TTCTGGAGAA TGGGAGCTGC TGAGGCTGGA TTCCAGCAAG 
AAGGAACTGC CCAGCCCATG CCCAGACAAG GACAGTGACA AGGACCTTGG TCCTCGGCTC 
CAGCTCCCCT CAGCCCCCGT AGCCACTGGT CTTTCTACCC TGGACTCCAT CTGTGACTCC 
CTGAGTGTTG CTTTCCGGGG CATTAGTCAC TGTCCTCCTA GTGGGCTCTA TGCCCACCTC 
TGCCGCTTCC TGGCCTTGTG CCTGGGCCAC CGGGATCCTT ATGCCACTGC TTTCCTTGTC 
ACCGAGTCTG TCTCCATCAC GTGTCGCCAC CAGCTGCTCA CCCACCTCCA CAGACAGCTC 
AGCAAGACCC AGAAGCACCG AGGATCACTT GAAATAGCAG ACCAGCTGCA GAGGCTGAGC 
CTTCAGGAGA CGCCTAGAGA TGTCCCCCTG GCCCGCATCC AGCGCCTCTT TTCCTTCAGG 
GCTTTGGGAT CTGGCCACTT CCCCCAGCCT GAAAAGGAGA GTTTCCAGGA GCGCCTGGCT 
CTGATCCCCA GTGGGGTGAC TGTGTGTGTG TTGGCCCTGG CCACCCTCCA GCCCGGAACC 
GTGGGCAACA CCCTCCTGCT GACCCGGCTG GAAAAGGACA GTCCCCCAGT CAGTGTGCAG 
ATTCCCACTG GCCAGAACAA GCTTCCTCTG CGTTCAGTCC TGAATGAATT CGATGCCATC 
CAGAAGGCAC AGAAAGAGAA CAGCAGCTGT ACTGACAAGC GAGAATGGTG GACGGGGCGG 
CTGGCACTGG ACCACAGGAT GGAGGTTCTC ATCGCTTCCC TAGAGAAGTC TGTGCTGGGC 
TGCTGGAAGG GGCTGCTGCT GCCGTCCAGT GAGGAGCCCG GCCCTGCCCA GGAGGCCTCC 
CGCCTACAGG AGCTGCTACA GGAATGTGGC TGGAAATATC CTGACCCCAC TCTGCTGAAA 
TATGGGGCCT CGCCAGTGCT GAGTCAAGGG GTGAATCCGC GAAGTACCTT CTATGTCCTG 
AACCCTCACA ATAACCTGTC AAGCACAGAG GAGCAATTTC GAGCCAATTT CAGCAGTGAA 
GCTGGCTGGA GAGGAGTGGT TGGGGAGGTG CCAAGACCTG AACAGGTGCA AGAAGCCCTG 
ACAAAGCATG ATTTGTATAT CTATGCAGGG CATGGGGCTG GTGCCCGCTT CCTTGACGGG 
CAGGCTGTCC TGCGGCTGAG CTGTCGGGCA GTGGCCCTGC TGTTTGGCTG TAGCAGTGCG 
GCCCTGGCTG TGCATGGAAA CCTGGAGGGG GCTGGCATTG TGCTCAAGTA CATCATGGCT 
GGTTGCCCCT TGTTTCTGGG TAATCTCTGG GATGTGACTG ACCGTGACAT TGACCGCTAC 
ACGGAAGCTC TGCTGCAGGG CTGGCTTGGA GCGGGCCCAG GGGCCCCCCT TCTCTACTAT 
GTAAACCAGG CCCGCCAAGC TCCCCGACTC AAGTATCTTA TTGGGGCTGC ACCTATAGCC 
TATGGCTTGC CTGTCTCTCT GCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESPL1 ( XM_009247812.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009246087.1 Pongo abelii extra spindle pole bodies homolog 1 (S. cerevisiae) (ESPL1), mRNA.

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CloneID	OSa10466
Clone ID Related Accession (Same CDS sequence)	XM_009247812.1
Accession Version	XM_009247812.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	separin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002885841.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCGGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTGGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC AATCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAGA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTACAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT ACGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATATA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGAGAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACTACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCTGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAACCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA AGAGCTGCAG GCTTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCTGGGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GAGTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGCAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGTTACAC CTGGAAGAGG CAGCATCGCG CCTGAAGCAT 
CTCAATCAGA CCACTGACAC ATACCTGCTC CTTTCCCTGA CCTGTGATCT GCTTCGAAGT 
CAACTCTACT GGACTCACCA GAAGGTGACT GAGGGTGTCT CTCTGCTGCT GTCTGTGCTT 
CGGGATCCTG CCCTCCAGAA GTCCTCCAAG GCTTGGTACT TGCTGCGTGT CCAGGTCCTG 
CAGCTGGTGG CAGCTTACCT TAGCCTCCCG TCAAACAGCC TCTCACACTC CCTGTGGGAG 
CAGCTCTGTG CCCAAGGCTG GCAGACGCCT GAGATAGCTC TCATAGACTC CCATAAGCTC 
CTCCGAAGCA TCATCCTCCT GCTGATGGGC AGTGACATTC TCTCAACTCA GAAGGCAGCT 
GTGGAGACAT CGTTTTTGGA CTATGGTGAA AATCTGGTAC AAAAATGGCA GGTTCTTTCA 
GAGGTGCTGA GCTGCTCAGA GAAGCTGGTC TGCCACCTGG GCCGCCTGGG TAGCGTGAGT 
GAAGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACAA CAAAGCTGCA GATACCACGC 
CAGTGTGCCC TGTTCCTGGT GCTGAAGGGC GAGCTGGAGC TGGCCCGCAA TGACATTGAT 
CTCTGTCAGT CGGACCTGCA GCAGGTTCTG TTCTTGCTTG AGTCTTGCAC AGAGTTTGGT 
GGGGTGACTC AGCACCTGGA CTCTGTGAAG AAGGTCCACC TGCAGAAGGG GAAGCAGCAG 
GCCCAGGTCC CCTGTCCTCC ACAGCTCCCA GAGGAGGAGC TCTTCCTAAG AGGCCCTGCT 
CTAGAGCTGG TGGCCACTGT GGCCAAGGAG CCTGGCCCCA TAGCACCTTC TACAAACTCC 
TCCCCAGTCT TGAAAACCAA GCCCCAGCCC ATACCCAACT TCCTGTCCCA TTCACCCACC 
TGTGACTGCT CGCTCTGCGC CAGCCCTGTC CTCACAGCAG TCTGTCTGCG CTGGGTATTG 
GTCACGGCAG GGGTGAGGCT GGCCATGGGC CACCAGGCCC AGGGTCTGGA TCTGCTGCAG 
GTCGTGCTGA AGGGCTGTCC TGAAGCCGCC GAGCGCCTCA CCCAAGCTCT CCAAGCTTCC 
CTGAATCATA AAACACCCCC CTCCTTGGTT CCAAGCCTCT TGGATGAGAT CTTGGCTCAA 
GCATACACAC TGTTGGCACT GGAGGGCCTG AGCCAGCCAT CAAACAAGAG CCTGCAGAAG 
GTTCTAGAGT CAGGGCTGAA GTTTGTAGCA GCACGGATAC CCCACCTAGA GCCCTGGCGA 
GCCAGCCTGC TCTTGATTTG GGCCCTCACA AAGCTAGCTG GCCTCAGCTG CTGTACTACC 
CAACTTTTTG CAAGCTCCTG GGGCTGGCGG CCACCATTAA TAAAAAGTGT CCCTGGCTCA 
GAGCCCTCTA AGACTCAGGG CCAAAAACGT TCTGGACGAG GGCGCCAAAA GGTAGCCTCT 
GCTCCCCTGA GCCTCAATAA TACCTCTCAG AAAGGTCTGG AAGGTAGAGG ACTTCCCTGC 
ACACCTAAAC CCCCAGGCCG AATCAGGCAA GCTGGCCCTC ATGTCCCCTT CACGGTGTTT 
GAGGAAGTCT GCCCTACAAA GAGCAAGCCT GAAGTTCCCC AGGCCCCCAG GGTACAACAG 
AGAGTCCAGA CGCGCCTCAA GGTGAACTTC AGTGATGACA GTGACTTGGA AGACCCTGTC 
TCAGCTGAGG CCTGGCTGGC AGAGGAGGCT AAGAGACGGG GCACTGCTTC CCGGGGCCGG 
GGGCGAGCAA GGAAGGGTAT GAGCCTAAAG ACGGATGCCG TGGTTGCCCC AGGTAGTGCC 
CCTGGGAACC CTGGCCTGAG TGGCAGGAGC TGGAGGGCCA AGAAGGTGGC ATCAAGACAT 
TGTGAGGAGC AGCGTCCCCG GAGGGCCAAT GACCAGGCCA GGCCTGGCCC TGAGATCATG 
AGGACCATCC CTGAGGAGGA ACTGACTGAC AACTGGAGAG AAATGAGCTT TGAGATCCTC 
AGGGGCTCTG ATGGGGAAGA CTCAGCCTCA GGTGGGAAGG CTCCAGCGCC GGGCCCTGAG 
GCAGCTTCTG GAGAATGGGA GCTGCTGAGG CTGGATTCCA GCAAGAAGGA ACTGCCCAGC 
CCATGCCCAG ACAAGGACAG TGACAAGGAC CTTGGTCCTC GGCTCCAGCT CCCCTCAGCC 
CCCGTAGCCA CTGGTCTTTC TACCCTGGAC TCCATCTGTG ACTCCCTGAG TGTTGCTTTC 
CGGGGCATTA GTCACTGTCC TCCTAGTGGG CTCTATGCCC ACCTCTGCCG CTTCCTGGCC 
TTGTGCCTGG GCCACCGGGA TCCTTATGCC ACTGCTTTCC TTGTCACCGA GTCTGTCTCC 
ATCACGTGTC GCCACCAGCT GCTCACCCAC CTCCACAGAC AGCTCAGCAA GACCCAGAAG 
CACCGAGGAT CACTTGAAAT AGCAGACCAG CTGCAGAGGC TGAGCCTTCA GGAGACGCCT 
AGAGATGTCC CCCTGGCCCG CATCCAGCGC CTCTTTTCCT TCAGGGCTTT GGGATCTGGC 
CACTTCCCCC AGCCTGAAAA GGAGAGTTTC CAGGAGCGCC TGGCTCTGAT CCCCAGTGGG 
GTGACTGTGT GTGTGTTGGC CCTGGCCACC CTCCAGCCCG GAACCGTGGG CAACACCCTC 
CTGCTGACCC GGCTGGAAAA GGACAGTCCC CCAGTCAGTG TGCAGATTCC CACTGGCCAG 
AACAAGCTTC CTCTGCGTTC AGTCCTGAAT GAATTCGATG CCATCCAGAA GGCACAGAAA 
GAGAACAGCA GCTGTACTGA CAAGCGAGAA TGGTGGACGG GGCGGCTGGC ACTGGACCAC 
AGGATGGAGG TTCTCATCGC TTCCCTAGAG AAGTCTGTGC TGGGCTGCTG GAAGGGGCTG 
CTGCTGCCGT CCAGTGAGGA GCCCGGCCCT GCCCAGGAGG CCTCCCGCCT ACAGGAGCTG 
CTACAGGAAT GTGGCTGGAA ATATCCTGAC CCCACTCTGC TGAAAATCAT GCTCAGTGGT 
GCCGGTGCCC TCACCCCTCA GGACATTCAG GCCCTGGCCT ACGGGCTGTG CCCAACCCAG 
CCAAAGCGAG CCCAGGAGCT CCTGAATGAG GCAGTAGGAC GGCTACAGGG CCTGACAGTA 
CCAGGCAATA GCCACCTTGT CTTGGTCCTA GACAAGGACT TGCAGAAGCT GCCGTGGGAA 
AGCATGCCCA GCCTCCAGGC ACTGCCTGTC ACCCGGCTGC CCTCCTTCCG CTTCCTACTC 
AGCTACTCCA TCATCAAAGA GTATGGGGCC TCGCCAGTGC TGAGTCAAGG GGTGAATCCG 
CGAAGTACCT TCTATGTCCT GAACCCTCAC AATAACCTGT CAAGCACAGA GGAGCAATTT 
CGAGCCAATT TCAGCAGTGA AGCTGGCTGG AGAGGAGTGG TTGGGGAGGT GCCAAGACCT 
GAACAGGTGC AAGAAGCCCT GACAAAGCAT GATTTGTATA TCTATGCAGG GCATGGGGCT 
GGTGCCCGCT TCCTTGACGG GCAGGCTGTC CTGCGGCTGA GCTGTCGGGC AGTGGCCCTG 
CTGTTTGGCT GTAGCAGTGC GGCCCTGGCT GTGCATGGAA ACCTGGAGGG GGCTGGCATT 
GTGCTCAAGT ACATCATGGC TGGTTGCCCC TTGTTTCTGG GTAATCTCTG GGATGTGACT 
GACCGTGACA TTGACCGCTA CACGGAAGCT CTGCTGCAGG GCTGGCTTGG AGCGGGCCCA 
GGGGCCCCCC TTCTCTACTA TGTAAACCAG GCCCGCCAAG CTCCCCGACT CAAGTATCTT 
ATTGGGGCTG CACCTATAGC CTATGGCTTG CCTGTCTCTC TGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009246087.1
CDS	361..6588
Translation	MRSFKRVNFGTLLSSRKEAEELLPDLKEFLSNPPAGFPSSRSDAERRQACDAILRACNQQLTAKLACPRHLGSLLELAELACDGYLVSTPQRPPLYLERILFVLLRNAAAQGSPEATIRLAQPLHACLVQCSREAAPQDYEAVARGSFSLLWKGAEALLERRAAFAARLKALSFLVLLEDESTPCEVPHFASPTACRVVAAHQLFDASGHGLNEADADFLDDLLSRHVIRALVGERGSSSGLLSPQRALCLLELTLEHCRRFCWSRHHDRAISAVEKAHSYLRNTNLAPSLQLCQLGVKLLQVGEEGPQAVAKLLIKASAVLSKSMEAPSPPLRALYESCQFFLSGLERGTKRRYRLDAILSLFAFLGGYCSLLQQLRDDGVYGGSSKQQQSFLQIYFQGLHLYTVVVYDFAQGCQIVDLADLTQLVESCKSTVVWMLEALEGLSGQELTDYMGMTASYTSNLAYSFYSHKLYAEACAISEPLCQHLGLVKPGTYPEVPPEKLHRCFRLQVESLKKLGKQAQGCKMVILWLAALQPCSPEHMAEPVTFWVRVKMDAARAGDKELQLKTLRDNLSGWDPETLALLLREELQAYKAVRADTGQERFNIICDLLELSPGETPAGAWARATHLVELAQVLCYHDFTQQTNCSALDAIREALQLLESVRPEAQARDQLLDDKAQALLWLYICTLEAKMQEGIERDRRAQAPGNLEEFEVNDLNYEDKLQEDRFLYSNIAFNLAADAAQSKCLDQALALWKELLTKGQAPAVRCLQQTAASLQILAALYQLVAKLHLEEAASRLKHLNQTTDTYLLLSLTCDLLRSQLYWTHQKVTEGVSLLLSVLRDPALQKSSKAWYLLRVQVLQLVAAYLSLPSNSLSHSLWEQLCAQGWQTPEIALIDSHKLLRSIILLLMGSDILSTQKAAVETSFLDYGENLVQKWQVLSEVLSCSEKLVCHLGRLGSVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARNDIDLCQSDLQQVLFLLESCTEFGGVTQHLDSVKKVHLQKGKQQAQVPCPPQLPEEELFLRGPALELVATVAKEPGPIAPSTNSSPVLKTKPQPIPNFLSHSPTCDCSLCASPVLTAVCLRWVLVTAGVRLAMGHQAQGLDLLQVVLKGCPEAAERLTQALQASLNHKTPPSLVPSLLDEILAQAYTLLALEGLSQPSNKSLQKVLESGLKFVAARIPHLEPWRASLLLIWALTKLAGLSCCTTQLFASSWGWRPPLIKSVPGSEPSKTQGQKRSGRGRQKVASAPLSLNNTSQKGLEGRGLPCTPKPPGRIRQAGPHVPFTVFEEVCPTKSKPEVPQAPRVQQRVQTRLKVNFSDDSDLEDPVSAEAWLAEEAKRRGTASRGRGRARKGMSLKTDAVVAPGSAPGNPGLSGRSWRAKKVASRHCEEQRPRRANDQARPGPEIMRTIPEEELTDNWREMSFEILRGSDGEDSASGGKAPAPGPEAASGEWELLRLDSSKKELPSPCPDKDSDKDLGPRLQLPSAPVATGLSTLDSICDSLSVAFRGISHCPPSGLYAHLCRFLALCLGHRDPYATAFLVTESVSITCRHQLLTHLHRQLSKTQKHRGSLEIADQLQRLSLQETPRDVPLARIQRLFSFRALGSGHFPQPEKESFQERLALIPSGVTVCVLALATLQPGTVGNTLLLTRLEKDSPPVSVQIPTGQNKLPLRSVLNEFDAIQKAQKENSSCTDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLPSSEEPGPAQEASRLQELLQECGWKYPDPTLLKIMLSGAGALTPQDIQALAYGLCPTQPKRAQELLNEAVGRLQGLTVPGNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPSFRFLLSYSIIKEYGASPVLSQGVNPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPLLYYVNQARQAPRLKYLIGAAPIAYGLPVSLR

Target ORF information:

RefSeq Version	XM_009247812.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii extra spindle pole bodies homolog 1 (S. cerevisiae) (ESPL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009247812.1

ORF Insert Sequence:

ATGAGGAGCT TCAAAAGAGT CAACTTTGGG ACTCTGCTAA GCAGCCGGAA GGAGGCTGAA 
GAGTTGCTGC CCGACTTGAA GGAGTTCCTG TCCAACCCTC CAGCTGGTTT TCCCAGCAGC 
CGATCTGATG CTGAGAGGAG ACAAGCTTGT GATGCCATCC TGAGGGCTTG CAACCAGCAG 
CTGACTGCTA AGCTAGCTTG CCCTAGGCAT CTGGGGAGCC TGCTGGAGCT GGCAGAGCTG 
GCCTGTGATG GCTACTTGGT GTCTACCCCA CAGCGTCCTC CCCTCTACCT GGAACGAATT 
CTCTTCGTCT TACTGCGGAA TGCTGCTGCA CAAGGAAGCC CAGAGGCCAC AATCCGCCTT 
GCTCAGCCCC TCCATGCCTG CTTGGTGCAG TGCTCTCGCG AGGCTGCTCC CCAGGACTAT 
GAGGCCGTGG CTCGGGGCAG CTTTTCTCTG CTTTGGAAGG GGGCAGAAGC CCTGTTGGAA 
CGGCGAGCTG CATTTGCAGC TCGGCTGAAG GCCTTGAGCT TCCTAGTACT CTTGGAGGAT 
GAAAGTACCC CTTGTGAGGT TCCTCACTTT GCTTCTCCAA CAGCCTGTCG AGTGGTAGCT 
GCCCATCAGC TATTTGATGC CAGTGGCCAT GGTCTAAATG AAGCAGATGC TGATTTCCTA 
GATGACCTGC TCTCCAGGCA CGTGATCAGA GCCTTGGTGG GTGAGAGAGG GAGCTCTTCT 
GGGCTTCTTT CTCCCCAGAG GGCCCTCTGC CTCTTGGAGC TCACCTTGGA ACACTGCCGT 
CGCTTTTGCT GGAGCCGCCA CCATGACAGA GCCATCAGCG CAGTGGAGAA GGCTCACAGT 
TACCTAAGGA ACACCAATCT AGCCCCTAGC CTTCAGCTAT GTCAGCTGGG GGTTAAGCTG 
CTGCAGGTTG GGGAGGAAGG ACCTCAGGCA GTGGCCAAGC TTCTGATCAA GGCATCAGCT 
GTCCTGAGCA AGAGTATGGA GGCACCATCA CCCCCACTTC GGGCATTGTA TGAGAGCTGC 
CAGTTCTTCC TTTCAGGCCT GGAACGAGGC ACCAAGAGGC GCTACAGACT TGATGCCATT 
CTGAGCCTCT TTGCTTTTCT TGGAGGGTAC TGCTCTCTTC TGCAGCAGCT ACGGGATGAT 
GGTGTGTATG GGGGCTCCTC CAAGCAACAG CAGTCTTTTC TTCAGATATA CTTTCAGGGA 
CTTCACCTCT ACACTGTGGT GGTTTATGAC TTTGCCCAAG GCTGTCAGAT AGTTGATTTG 
GCTGACCTGA CCCAACTAGT GGAGAGTTGT AAATCTACCG TTGTCTGGAT GCTGGAGGCC 
TTAGAGGGCC TGTCGGGCCA AGAGCTGACG GACTACATGG GGATGACCGC TTCTTACACC 
AGTAATTTGG CCTACAGCTT CTATAGTCAC AAGCTCTATG CCGAGGCCTG TGCCATCTCC 
GAGCCGCTCT GTCAGCACCT GGGTTTGGTG AAGCCAGGCA CTTATCCTGA GGTGCCTCCT 
GAGAAGTTGC ACAGGTGCTT CCGGCTACAA GTAGAGAGTT TGAAGAAACT GGGTAAACAG 
GCCCAGGGCT GCAAGATGGT GATTTTGTGG CTGGCAGCCC TGCAACCCTG TAGCCCTGAA 
CACATGGCTG AGCCAGTCAC TTTCTGGGTT CGGGTCAAGA TGGATGCGGC CAGGGCTGGA 
GACAAGGAGC TACAGCTAAA GACTCTGCGA GACAACCTCA GTGGCTGGGA CCCGGAGACC 
CTGGCCCTCC TGCTGAGGGA AGAGCTGCAG GCTTACAAGG CGGTGCGGGC CGACACTGGA 
CAGGAACGCT TCAACATCAT CTGTGACCTC CTGGAGCTGA GCCCTGGGGA GACACCAGCC 
GGGGCCTGGG CACGAGCCAC CCACCTGGTA GAACTGGCTC AGGTGCTCTG CTACCACGAC 
TTTACGCAGC AGACCAACTG CTCTGCTCTG GATGCTATCC GGGAAGCCCT GCAGCTTCTG 
GAGTCTGTGA GGCCTGAGGC CCAGGCCAGA GATCAGCTTC TGGACGATAA AGCACAGGCC 
TTGCTGTGGC TTTACATCTG TACTCTGGAA GCCAAAATGC AGGAAGGCAT CGAGCGGGAT 
CGGAGAGCCC AGGCCCCTGG TAACTTGGAG GAATTTGAAG TCAATGACCT GAACTATGAA 
GATAAACTCC AGGAAGATCG TTTCCTATAC AGTAACATTG CCTTCAACCT GGCTGCAGAT 
GCTGCTCAGT CCAAATGCCT GGACCAAGCC CTGGCCCTGT GGAAGGAGCT GCTTACAAAG 
GGGCAGGCCC CAGCTGTACG GTGTCTCCAG CAGACAGCAG CCTCACTGCA GATCCTAGCA 
GCCCTCTACC AGCTGGTGGC AAAGTTACAC CTGGAAGAGG CAGCATCGCG CCTGAAGCAT 
CTCAATCAGA CCACTGACAC ATACCTGCTC CTTTCCCTGA CCTGTGATCT GCTTCGAAGT 
CAACTCTACT GGACTCACCA GAAGGTGACT GAGGGTGTCT CTCTGCTGCT GTCTGTGCTT 
CGGGATCCTG CCCTCCAGAA GTCCTCCAAG GCTTGGTACT TGCTGCGTGT CCAGGTCCTG 
CAGCTGGTGG CAGCTTACCT TAGCCTCCCG TCAAACAGCC TCTCACACTC CCTGTGGGAG 
CAGCTCTGTG CCCAAGGCTG GCAGACGCCT GAGATAGCTC TCATAGACTC CCATAAGCTC 
CTCCGAAGCA TCATCCTCCT GCTGATGGGC AGTGACATTC TCTCAACTCA GAAGGCAGCT 
GTGGAGACAT CGTTTTTGGA CTATGGTGAA AATCTGGTAC AAAAATGGCA GGTTCTTTCA 
GAGGTGCTGA GCTGCTCAGA GAAGCTGGTC TGCCACCTGG GCCGCCTGGG TAGCGTGAGT 
GAAGCCAAGG CCTTTTGCTT GGAGGCCCTA AAACTTACAA CAAAGCTGCA GATACCACGC 
CAGTGTGCCC TGTTCCTGGT GCTGAAGGGC GAGCTGGAGC TGGCCCGCAA TGACATTGAT 
CTCTGTCAGT CGGACCTGCA GCAGGTTCTG TTCTTGCTTG AGTCTTGCAC AGAGTTTGGT 
GGGGTGACTC AGCACCTGGA CTCTGTGAAG AAGGTCCACC TGCAGAAGGG GAAGCAGCAG 
GCCCAGGTCC CCTGTCCTCC ACAGCTCCCA GAGGAGGAGC TCTTCCTAAG AGGCCCTGCT 
CTAGAGCTGG TGGCCACTGT GGCCAAGGAG CCTGGCCCCA TAGCACCTTC TACAAACTCC 
TCCCCAGTCT TGAAAACCAA GCCCCAGCCC ATACCCAACT TCCTGTCCCA TTCACCCACC 
TGTGACTGCT CGCTCTGCGC CAGCCCTGTC CTCACAGCAG TCTGTCTGCG CTGGGTATTG 
GTCACGGCAG GGGTGAGGCT GGCCATGGGC CACCAGGCCC AGGGTCTGGA TCTGCTGCAG 
GTCGTGCTGA AGGGCTGTCC TGAAGCCGCC GAGCGCCTCA CCCAAGCTCT CCAAGCTTCC 
CTGAATCATA AAACACCCCC CTCCTTGGTT CCAAGCCTCT TGGATGAGAT CTTGGCTCAA 
GCATACACAC TGTTGGCACT GGAGGGCCTG AGCCAGCCAT CAAACAAGAG CCTGCAGAAG 
GTTCTAGAGT CAGGGCTGAA GTTTGTAGCA GCACGGATAC CCCACCTAGA GCCCTGGCGA 
GCCAGCCTGC TCTTGATTTG GGCCCTCACA AAGCTAGCTG GCCTCAGCTG CTGTACTACC 
CAACTTTTTG CAAGCTCCTG GGGCTGGCGG CCACCATTAA TAAAAAGTGT CCCTGGCTCA 
GAGCCCTCTA AGACTCAGGG CCAAAAACGT TCTGGACGAG GGCGCCAAAA GGTAGCCTCT 
GCTCCCCTGA GCCTCAATAA TACCTCTCAG AAAGGTCTGG AAGGTAGAGG ACTTCCCTGC 
ACACCTAAAC CCCCAGGCCG AATCAGGCAA GCTGGCCCTC ATGTCCCCTT CACGGTGTTT 
GAGGAAGTCT GCCCTACAAA GAGCAAGCCT GAAGTTCCCC AGGCCCCCAG GGTACAACAG 
AGAGTCCAGA CGCGCCTCAA GGTGAACTTC AGTGATGACA GTGACTTGGA AGACCCTGTC 
TCAGCTGAGG CCTGGCTGGC AGAGGAGGCT AAGAGACGGG GCACTGCTTC CCGGGGCCGG 
GGGCGAGCAA GGAAGGGTAT GAGCCTAAAG ACGGATGCCG TGGTTGCCCC AGGTAGTGCC 
CCTGGGAACC CTGGCCTGAG TGGCAGGAGC TGGAGGGCCA AGAAGGTGGC ATCAAGACAT 
TGTGAGGAGC AGCGTCCCCG GAGGGCCAAT GACCAGGCCA GGCCTGGCCC TGAGATCATG 
AGGACCATCC CTGAGGAGGA ACTGACTGAC AACTGGAGAG AAATGAGCTT TGAGATCCTC 
AGGGGCTCTG ATGGGGAAGA CTCAGCCTCA GGTGGGAAGG CTCCAGCGCC GGGCCCTGAG 
GCAGCTTCTG GAGAATGGGA GCTGCTGAGG CTGGATTCCA GCAAGAAGGA ACTGCCCAGC 
CCATGCCCAG ACAAGGACAG TGACAAGGAC CTTGGTCCTC GGCTCCAGCT CCCCTCAGCC 
CCCGTAGCCA CTGGTCTTTC TACCCTGGAC TCCATCTGTG ACTCCCTGAG TGTTGCTTTC 
CGGGGCATTA GTCACTGTCC TCCTAGTGGG CTCTATGCCC ACCTCTGCCG CTTCCTGGCC 
TTGTGCCTGG GCCACCGGGA TCCTTATGCC ACTGCTTTCC TTGTCACCGA GTCTGTCTCC 
ATCACGTGTC GCCACCAGCT GCTCACCCAC CTCCACAGAC AGCTCAGCAA GACCCAGAAG 
CACCGAGGAT CACTTGAAAT AGCAGACCAG CTGCAGAGGC TGAGCCTTCA GGAGACGCCT 
AGAGATGTCC CCCTGGCCCG CATCCAGCGC CTCTTTTCCT TCAGGGCTTT GGGATCTGGC 
CACTTCCCCC AGCCTGAAAA GGAGAGTTTC CAGGAGCGCC TGGCTCTGAT CCCCAGTGGG 
GTGACTGTGT GTGTGTTGGC CCTGGCCACC CTCCAGCCCG GAACCGTGGG CAACACCCTC 
CTGCTGACCC GGCTGGAAAA GGACAGTCCC CCAGTCAGTG TGCAGATTCC CACTGGCCAG 
AACAAGCTTC CTCTGCGTTC AGTCCTGAAT GAATTCGATG CCATCCAGAA GGCACAGAAA 
GAGAACAGCA GCTGTACTGA CAAGCGAGAA TGGTGGACGG GGCGGCTGGC ACTGGACCAC 
AGGATGGAGG TTCTCATCGC TTCCCTAGAG AAGTCTGTGC TGGGCTGCTG GAAGGGGCTG 
CTGCTGCCGT CCAGTGAGGA GCCCGGCCCT GCCCAGGAGG CCTCCCGCCT ACAGGAGCTG 
CTACAGGAAT GTGGCTGGAA ATATCCTGAC CCCACTCTGC TGAAAATCAT GCTCAGTGGT 
GCCGGTGCCC TCACCCCTCA GGACATTCAG GCCCTGGCCT ACGGGCTGTG CCCAACCCAG 
CCAAAGCGAG CCCAGGAGCT CCTGAATGAG GCAGTAGGAC GGCTACAGGG CCTGACAGTA 
CCAGGCAATA GCCACCTTGT CTTGGTCCTA GACAAGGACT TGCAGAAGCT GCCGTGGGAA 
AGCATGCCCA GCCTCCAGGC ACTGCCTGTC ACCCGGCTGC CCTCCTTCCG CTTCCTACTC 
AGCTACTCCA TCATCAAAGA GTATGGGGCC TCGCCAGTGC TGAGTCAAGG GGTGAATCCG 
CGAAGTACCT TCTATGTCCT GAACCCTCAC AATAACCTGT CAAGCACAGA GGAGCAATTT 
CGAGCCAATT TCAGCAGTGA AGCTGGCTGG AGAGGAGTGG TTGGGGAGGT GCCAAGACCT 
GAACAGGTGC AAGAAGCCCT GACAAAGCAT GATTTGTATA TCTATGCAGG GCATGGGGCT 
GGTGCCCGCT TCCTTGACGG GCAGGCTGTC CTGCGGCTGA GCTGTCGGGC AGTGGCCCTG 
CTGTTTGGCT GTAGCAGTGC GGCCCTGGCT GTGCATGGAA ACCTGGAGGG GGCTGGCATT 
GTGCTCAAGT ACATCATGGC TGGTTGCCCC TTGTTTCTGG GTAATCTCTG GGATGTGACT 
GACCGTGACA TTGACCGCTA CACGGAAGCT CTGCTGCAGG GCTGGCTTGG AGCGGGCCCA 
GGGGCCCCCC TTCTCTACTA TGTAAACCAG GCCCGCCAAG CTCCCCGACT CAAGTATCTT 
ATTGGGGCTG CACCTATAGC CTATGGCTTG CCTGTCTCTC TGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ESPL1 cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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