Spi1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spi1
Entrez Gene ID	366126
Full Name	Spi-1 proto-oncogene
Synonyms	Pu.1,Sfpi1
General protein information	Preferred Names Spi-1 proto-oncogene Names transcription factor PU.1 SFFV proviral integration 1 hematopoietic transcription factor PU.1 spleen focus forming virus (SFFV) proviral integration oncogene spi1 spleen focus forming virus proviral integration oncogene spi1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_001005892.2	NP_001005892.1	transcription factor PU.1
XM_006234532.3	XP_006234594.1	transcription factor PU.1 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	spi1b	NP_932328.1
Canis lupus familiaris (dog)	SPI1	XP_853990.1
Rattus norvegicus (Norway rat)	Spi1	NP_001005892.1
Homo sapiens (human)	SPI1	NP_001074016.1
Mus musculus (house mouse)	Spi1	NP_035485.1
Xenopus tropicalis (tropical clawed frog)	spi1	NP_001139455.1
Bos taurus (cattle)	SPI1	NP_001179062.1
Gallus gallus (chicken)	SPI1	NP_990354.1

Gene Ontology
Bibliography

Related articles in PubMed

Nrf2 regulates PU.1 expression and activity in the alveolar macrophage.
Staitieh BS, Fan X, Neveu W, Guidot DM
American journal of physiology. Lung cellular and molecular physiology308(10)L1086-93(2015 May)

Playing TETris with DNA modifications.
Delatte B, Deplus R, Fuks F
The EMBO journal33(11)1198-211(2014 Jun)

Hepatitis C virus-induced changes in microRNA 107 (miRNA-107) and miRNA-449a modulate CCL2 by targeting the interleukin-6 receptor complex in hepatitis.
Sarma NJ, Tiriveedhi V, Crippin JS, Chapman WC, Mohanakumar T
Journal of virology88(7)3733-43(2014 Apr)

PU.1-silenced dendritic cells prolong allograft survival in rats receiving intestinal transplantation.
Xu XW, Ding BW, Zhu CR, Ji W, Li JS
World journal of gastroenterology19(43)7766-71(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nrf2 regulates the expression and activity of PU.1 and that antioxidant response and immune activation are coordinately regulated within the AM.
Title: Nrf2 regulates PU.1 expression and activity in the alveolar macrophage.

PU.1-silenced semi-mature dendritic cells induced partial immune tolerance both in vitro and in vivo, which could be used as a new strategy to promote intestinal transplantation tolerance.
Title: PU.1-silenced dendritic cells prolong allograft survival in rats receiving intestinal transplantation.

interaction of mutants with IRF-8
Title: Analysis of PU.1/ICSBP (IRF-8) complex formation with various PU.1 mutants: molecular cloning of rat Icsbp (Irf-8) cDNA.

The following Spi1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spi1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13547	NM_001005892.2 Latest version!	Rattus norvegicus Spi-1 proto-oncogene (Spi1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa26091	XM_006234532.3 Latest version!	Rattus norvegicus Spi-1 proto-oncogene (Spi1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spi1 ( NM_001005892.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001005892.1 Rattus norvegicus Spi-1 proto-oncogene (Spi1), mRNA.

Price & Availability↑

CloneID	ORa13547
Clone ID Related Accession (Same CDS sequence)	NM_001005892.2
Accession Version	NM_001005892.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	transcription factor PU.1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC091185.1. On Mar 27, 2005 this sequence version replaced NM_001005892.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC091185.1, AB154364.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTTACAGG CGTGCAAAAT GGAAGGGTTT CCCCTCGTCG CCCCTCCATC GGATGAGCTG 
GTTACTTATG AATCAGAACT ATACCAACGT CAAACACATG ACTACTATTC CTTGATTGGT 
GGTGATGGAG ACAGCCATAG CGATCACTAT TGGGATTTCT CCACACACCA TGTACACAGC 
GAGTTTGAGA GCTTCCCTGA GAACCACTTC ACGGAGCTGC AGAGCGTGCA GCCCCCGCAG 
CTACAGCAGC TCTACCGCCA CATGGAGCTG GAGCAGATGC ACGTCCTTGA CACCCCCATG 
GCACCGCCCC ATGCCAGCCT CAGTCACCAG GTTTCCTACA TGCCCCGGGT GTGCTTCCCT 
TATCCACCCT TGTCCCCAGC CCACCAGCAG AGCTCAGATG AGGAGGAGGG TGAGAGGCAG 
AGCCCTCCAC TGGAGGTGTC TGATGGAGAA GCTGATGGCT TGGAGCCTGG GCCAGGCCTT 
CTGCATGGGG AGACAGGCAG CAAGAAGAAG ATTCGTTTGT ACCAGTTCCT GCTGGACCTG 
CTGCGTAGCG GAGACATGAA GGACAGCATC TGGTGGGTGG ACAAGGACAA AGGTACCTTC 
CAGTTCTCGT CCAAGCACAA GGAGGCGCTG GCGCACCGCT GGGGCATCCA GAAGGGAAAC 
CGCAAGAAGA TGACCTACCA GAAGATGGCG CGCGCGCTGC GCAACTACGG CAAGACGGGC 
GAGGTGAAGA AAGTCAAGAA GAAGCTCACT TACCAGTTCA GCGGCGAGGT GCTGGGCCGT 
GGGGGCCTGG CTGAGCGGCG CCTCCCGCCC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005892.1
CDS	156..971
Translation	MLQACKMEGFPLVAPPSDELVTYESELYQRQTHDYYSLIGGDGDSHSDHYWDFSTHHVHSEFESFPENHFTELQSVQPPQLQQLYRHMELEQMHVLDTPMAPPHASLSHQVSYMPRVCFPYPPLSPAHQQSSDEEEGERQSPPLEVSDGEADGLEPGPGLLHGETGSKKKIRLYQFLLDLLRSGDMKDSIWWVDKDKGTFQFSSKHKEALAHRWGIQKGNRKKMTYQKMARALRNYGKTGEVKKVKKKLTYQFSGEVLGRGGLAERRLPPH

Target ORF information:

RefSeq Version	NM_001005892.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Spi-1 proto-oncogene (Spi1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005892.2

ORF Insert Sequence:

ATGTTACAGG CGTGCAAAAT GGAAGGGTTT CCCCTCGTCG CCCCTCCATC GGATGAGCTG 
GTTACTTATG AATCAGAACT ATACCAACGT CAAACACATG ACTACTATTC CTTGATTGGT 
GGTGATGGAG ACAGCCATAG CGATCACTAT TGGGATTTCT CCACACACCA TGTACACAGC 
GAGTTTGAGA GCTTCCCTGA GAACCACTTC ACGGAGCTGC AGAGCGTGCA GCCCCCGCAG 
CTACAGCAGC TCTACCGCCA CATGGAGCTG GAGCAGATGC ACGTCCTTGA CACCCCCATG 
GCACCGCCCC ATGCCAGCCT CAGTCACCAG GTTTCCTACA TGCCCCGGGT GTGCTTCCCT 
TATCCACCCT TGTCCCCAGC CCACCAGCAG AGCTCAGATG AGGAGGAGGG TGAGAGGCAG 
AGCCCTCCAC TGGAGGTGTC TGATGGAGAA GCTGATGGCT TGGAGCCTGG GCCAGGCCTT 
CTGCATGGGG AGACAGGCAG CAAGAAGAAG ATTCGTTTGT ACCAGTTCCT GCTGGACCTG 
CTGCGTAGCG GAGACATGAA GGACAGCATC TGGTGGGTGG ACAAGGACAA AGGTACCTTC 
CAGTTCTCGT CCAAGCACAA GGAGGCGCTG GCGCACCGCT GGGGCATCCA GAAGGGAAAC 
CGCAAGAAGA TGACCTACCA GAAGATGGCG CGCGCGCTGC GCAACTACGG CAAGACGGGC 
GAGGTGAAGA AAGTCAAGAA GAAGCTCACT TACCAGTTCA GCGGCGAGGT GCTGGGCCGT 
GGGGGCCTGG CTGAGCGGCG CCTCCCGCCC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spi1 ( XM_006234532.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234594.1 Rattus norvegicus Spi-1 proto-oncogene (Spi1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa26091
Clone ID Related Accession (Same CDS sequence)	XM_006234532.3
Accession Version	XM_006234532.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transcription factor PU.1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234532.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACAGG CGTGCAAAAT GGAAGGGTTT CCCCTCGTCG CCCCTCAGCC ATCGGATGAG 
CTGGTTACTT ATGAATCAGA ACTATACCAA CGTCAAACAC ATGACTACTA TTCCTTGATT 
GGTGGTGATG GAGACAGCCA TAGCGATCAC TATTGGGATT TCTCCACACA CCATGTACAC 
AGCGAGTTTG AGAGCTTCCC TGAGAACCAC TTCACGGAGC TGCAGAGCGT GCAGCCCCCG 
CAGCTACAGC AGCTCTACCG CCACATGGAG CTGGAGCAGA TGCACGTCCT TGACACCCCC 
ATGGCACCGC CCCATGCCAG CCTCAGTCAC CAGGTTTCCT ACATGCCCCG GGTGTGCTTC 
CCTTATCCAC CCTTGTCCCC AGCCCACCAG CAGAGCTCAG ATGAGGAGGA GGGTGAGAGG 
CAGAGCCCTC CACTGGAGGT GTCTGATGGA GAAGCTGATG GCTTGGAGCC TGGGCCAGGC 
CTTCTGCATG GGGAGACAGG CAGCAAGAAG AAGATTCGTT TGTACCAGTT CCTGCTGGAC 
CTGCTGCGTA GCGGAGACAT GAAGGACAGC ATCTGGTGGG TGGACAAGGA CAAAGGTACC 
TTCCAGTTCT CGTCCAAGCA CAAGGAGGCG CTGGCGCACC GCTGGGGCAT CCAGAAGGGA 
AACCGCAAGA AGATGACCTA CCAGAAGATG GCGCGCGCGC TGCGCAACTA CGGCAAGACG 
GGCGAGGTGA AGAAAGTCAA GAAGAAGCTC ACTTACCAGT TCAGCGGCGA GGTGCTGGGC 
CGTGGGGGCC TGGCTGAGCG GCGCCTCCCG CCCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234594.1
CDS	1040..1858
Translation	MLQACKMEGFPLVAPQPSDELVTYESELYQRQTHDYYSLIGGDGDSHSDHYWDFSTHHVHSEFESFPENHFTELQSVQPPQLQQLYRHMELEQMHVLDTPMAPPHASLSHQVSYMPRVCFPYPPLSPAHQQSSDEEEGERQSPPLEVSDGEADGLEPGPGLLHGETGSKKKIRLYQFLLDLLRSGDMKDSIWWVDKDKGTFQFSSKHKEALAHRWGIQKGNRKKMTYQKMARALRNYGKTGEVKKVKKKLTYQFSGEVLGRGGLAERRLPPH

Target ORF information:

RefSeq Version	XM_006234532.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Spi-1 proto-oncogene (Spi1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234532.3

ORF Insert Sequence:

ATGTTACAGG CGTGCAAAAT GGAAGGGTTT CCCCTCGTCG CCCCTCAGCC ATCGGATGAG 
CTGGTTACTT ATGAATCAGA ACTATACCAA CGTCAAACAC ATGACTACTA TTCCTTGATT 
GGTGGTGATG GAGACAGCCA TAGCGATCAC TATTGGGATT TCTCCACACA CCATGTACAC 
AGCGAGTTTG AGAGCTTCCC TGAGAACCAC TTCACGGAGC TGCAGAGCGT GCAGCCCCCG 
CAGCTACAGC AGCTCTACCG CCACATGGAG CTGGAGCAGA TGCACGTCCT TGACACCCCC 
ATGGCACCGC CCCATGCCAG CCTCAGTCAC CAGGTTTCCT ACATGCCCCG GGTGTGCTTC 
CCTTATCCAC CCTTGTCCCC AGCCCACCAG CAGAGCTCAG ATGAGGAGGA GGGTGAGAGG 
CAGAGCCCTC CACTGGAGGT GTCTGATGGA GAAGCTGATG GCTTGGAGCC TGGGCCAGGC 
CTTCTGCATG GGGAGACAGG CAGCAAGAAG AAGATTCGTT TGTACCAGTT CCTGCTGGAC 
CTGCTGCGTA GCGGAGACAT GAAGGACAGC ATCTGGTGGG TGGACAAGGA CAAAGGTACC 
TTCCAGTTCT CGTCCAAGCA CAAGGAGGCG CTGGCGCACC GCTGGGGCAT CCAGAAGGGA 
AACCGCAAGA AGATGACCTA CCAGAAGATG GCGCGCGCGC TGCGCAACTA CGGCAAGACG 
GGCGAGGTGA AGAAAGTCAA GAAGAAGCTC ACTTACCAGT TCAGCGGCGA GGTGCTGGGC 
CGTGGGGGCC TGGCTGAGCG GCGCCTCCCG CCCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nrf2 regulates PU.1 expression and activity in the alveolar macrophage.
American journal of physiology. Lung cellular and molecular physiology308(10)L1086-93(2015 May)
Staitieh BS,Fan X,Neveu W,Guidot DM

Playing TETris with DNA modifications.
The EMBO journal33(11)1198-211(2014 Jun)
Delatte B,Deplus R,Fuks F

Hepatitis C virus-induced changes in microRNA 107 (miRNA-107) and miRNA-449a modulate CCL2 by targeting the interleukin-6 receptor complex in hepatitis.
Journal of virology88(7)3733-43(2014 Apr)
Sarma NJ,Tiriveedhi V,Crippin JS,Chapman WC,Mohanakumar T

PU.1-silenced dendritic cells prolong allograft survival in rats receiving intestinal transplantation.
World journal of gastroenterology19(43)7766-71(2013 Nov)
Xu XW,Ding BW,Zhu CR,Ji W,Li JS