SDHB cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	SDHB
Entrez Gene ID	414412
Full Name	succinate dehydrogenase complex iron sulfur subunit B
Synonyms	Ip
Gene Type	protein-coding
Organism	Sus scrofa(pig)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001104953.1	NP_001098423.1	succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Molecular cloning, sequence identification, and tissue expression profile analysis of three novel porcine genes: SDHB, SNRPA and CRYBB1.
An QC, Liu GY
Molecular biology reports36(4)683-90(2009 Apr)

Preliminary molecular characterization and crystallization of mitochondrial respiratory complex II from porcine heart.
Huo X, Su D, Wang A, Zhai Y, Xu J, Li X, Bartlam M, Sun F, Rao Z
The FEBS journal274(6)1524-9(2007 Mar)

PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences.
Uenishi H, Eguchi-Ogawa T, Shinkai H, Okumura N, Suzuki K, Toki D, Hamasima N, Awata T
Nucleic acids research35(Database issue)D650-3(2007 Jan)

Housekeeping genes for phylogenetic analysis of eutherian relationships.
Kullberg M, Nilsson MA, Arnason U, Harley EH, Janke A
Molecular biology and evolution23(8)1493-503(2006 Aug)

Crystal structure of mitochondrial respiratory membrane protein complex II.
Sun F, Huo X, Zhai Y, Wang A, Xu J, Su D, Bartlam M, Rao Z
Cell121(7)1043-57(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

tissue expression analysis indicated that that swine SDHB, SNRPA and CRYBB1 gene were differentially expressed in tissues including fat, lung, muscle, small intestine, kidney, large intestine, spleen and liver
Title: Molecular cloning, sequence identification, and tissue expression profile analysis of three novel porcine genes: SDHB, SNRPA and CRYBB1.

The gene sequences of each complex II subunit were measured by RT-PCR. N-terminal sequencing work was performed to identify the mitochondrial targeting signal peptide of each subunit.
Title: Preliminary molecular characterization and crystallization of mitochondrial respiratory complex II from porcine heart.

The following SDHB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SDHB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe00564	NM_001104953.1 Latest version!	Sus scrofa succinate dehydrogenase complex iron sulfur subunit B (SDHB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SDHB ( NM_001104953.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_001098423.1 Sus scrofa succinate dehydrogenase complex iron sulfur subunit B (SDHB), mRNA.

Price & Availability↑

CloneID	OSe00564
Clone ID Related Accession (Same CDS sequence)	NM_001104953.1
Accession Version	NM_001104953.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Sus scrofa(pig)
Product	succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ915498.1. ##Evidence-Data-START## Transcript exon combination :: DQ915498.1, AK232406.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02364669, SAMN02364670 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGCGG TGGTCGCGGT CTCCTTGAAA CGCTGGTTCC CGGCCACAAC CCTTGGCGGA 
GCCTGCCTGC AGGCCTGCCG TGGGGCCCAG ACAGCTGCAG CCACAGCTCC CCGAATCAAG 
AAATTTGCCA TCTATCGATG GGACCCAGAC AAGACTGGAG ATAAACCTCA TATGCAAACT 
TATGAAATTG ATTTGAATAA CTGTGGTCCT ATGGTGTTGG ATGCGTTAAT CAAGATTAAG 
AATGAAATTG ATTCTACCTT GACCTTCCGA AGATCGTGTA GAGAAGGCAT CTGCGGCTCC 
TGCGCCATGA ACATCAACGG AGGCAACACT CTGGCTTGCA CCCGAAGAAT TGACACCAAC 
CTCGACAAAG TTTCAAAAAT CTACCCTCTT CCACATATGT ATGTGATAAA GGATCTTGTT 
CCTGATTTGA GCAATTTCTA TGCTCAGTAC AAATCCATCG AGCCTTATCT AAAGAAGAAG 
GATGAATCCC AGGAAGGCAA GCAGCAGTAC CTGCAGTCCA TAGAGGAGCG CGAGAAACTG 
GATGGGCTGT ACGAGTGTAT TCTTTGCGCC TGCTGCAGCA CCAGCTGCCC GAGCTACTGG 
TGGAATGGAG ACAAGTACCT GGGACCCGCA GTCCTCATGC AGGCCTATCG CTGGATGATC 
GACTCCAGAG ATGACTTCAC GGAGGAGCGC CTGGCCAAGC TGCAGGACCC GTTCTCTCTG 
TACCGCTGCC ACACCATCAT GAACTGCACG GGGACCTGTC CCAAGGGGCT GAATCCAGGG 
AAAGCTATTG CTGAAATCAA GAAAATGATG GCAACCTACA AGGAGAAGAA AGCTTCAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001098423.1
CDS	1..843
Translation	MAAVVAVSLKRWFPATTLGGACLQACRGAQTAAATAPRIKKFAIYRWDPDKTGDKPHMQTYEIDLNNCGPMVLDALIKIKNEIDSTLTFRRSCREGICGSCAMNINGGNTLACTRRIDTNLDKVSKIYPLPHMYVIKDLVPDLSNFYAQYKSIEPYLKKKDESQEGKQQYLQSIEEREKLDGLYECILCACCSTSCPSYWWNGDKYLGPAVLMQAYRWMIDSRDDFTEERLAKLQDPFSLYRCHTIMNCTGTCPKGLNPGKAIAEIKKMMATYKEKKASA

Target ORF information:

RefSeq Version	NM_001104953.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa succinate dehydrogenase complex iron sulfur subunit B (SDHB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104953.1

ORF Insert Sequence:

ATGGCGGCGG TGGTCGCGGT CTCCTTGAAA CGCTGGTTCC CGGCCACAAC CCTTGGCGGA 
GCCTGCCTGC AGGCCTGCCG TGGGGCCCAG ACAGCTGCAG CCACAGCTCC CCGAATCAAG 
AAATTTGCCA TCTATCGATG GGACCCAGAC AAGACTGGAG ATAAACCTCA TATGCAAACT 
TATGAAATTG ATTTGAATAA CTGTGGTCCT ATGGTGTTGG ATGCGTTAAT CAAGATTAAG 
AATGAAATTG ATTCTACCTT GACCTTCCGA AGATCGTGTA GAGAAGGCAT CTGCGGCTCC 
TGCGCCATGA ACATCAACGG AGGCAACACT CTGGCTTGCA CCCGAAGAAT TGACACCAAC 
CTCGACAAAG TTTCAAAAAT CTACCCTCTT CCACATATGT ATGTGATAAA GGATCTTGTT 
CCTGATTTGA GCAATTTCTA TGCTCAGTAC AAATCCATCG AGCCTTATCT AAAGAAGAAG 
GATGAATCCC AGGAAGGCAA GCAGCAGTAC CTGCAGTCCA TAGAGGAGCG CGAGAAACTG 
GATGGGCTGT ACGAGTGTAT TCTTTGCGCC TGCTGCAGCA CCAGCTGCCC GAGCTACTGG 
TGGAATGGAG ACAAGTACCT GGGACCCGCA GTCCTCATGC AGGCCTATCG CTGGATGATC 
GACTCCAGAG ATGACTTCAC GGAGGAGCGC CTGGCCAAGC TGCAGGACCC GTTCTCTCTG 
TACCGCTGCC ACACCATCAT GAACTGCACG GGGACCTGTC CCAAGGGGCT GAATCCAGGG 
AAAGCTATTG CTGAAATCAA GAAAATGATG GCAACCTACA AGGAGAAGAA AGCTTCAGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular cloning, sequence identification, and tissue expression profile analysis of three novel porcine genes: SDHB, SNRPA and CRYBB1.
Molecular biology reports36(4)683-90(2009 Apr)
An QC,Liu GY

Preliminary molecular characterization and crystallization of mitochondrial respiratory complex II from porcine heart.
The FEBS journal274(6)1524-9(2007 Mar)
Huo X,Su D,Wang A,Zhai Y,Xu J,Li X,Bartlam M,Sun F,Rao Z

PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences.
Nucleic acids research35(Database issue)D650-3(2007 Jan)
Uenishi H,Eguchi-Ogawa T,Shinkai H,Okumura N,Suzuki K,Toki D,Hamasima N,Awata T

Housekeeping genes for phylogenetic analysis of eutherian relationships.
Molecular biology and evolution23(8)1493-503(2006 Aug)
Kullberg M,Nilsson MA,Arnason U,Harley EH,Janke A

Crystal structure of mitochondrial respiratory membrane protein complex II.
Cell121(7)1043-57(2005 Jul)
Sun F,Huo X,Zhai Y,Wang A,Xu J,Su D,Bartlam M,Rao Z