LOC660294 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC660294
Entrez Gene ID	660294
Full Name	60 kDa heat shock protein, mitochondrial
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_966537.4	XP_971630.1	60 kDa heat shock protein, mitochondrial

Pathways from BioSystems
Gene Ontology

The following LOC660294 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC660294 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTg06437	XM_966537.4 Latest version!	Tribolium castaneum 60 kDa heat shock protein, mitochondrial (LOC660294), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC660294 ( XM_966537.4 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_971630.1 Tribolium castaneum 60 kDa heat shock protein, mitochondrial (LOC660294), mRNA.

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CloneID	OTg06437
Clone ID Related Accession (Same CDS sequence)	XM_966537.4
Accession Version	XM_966537.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-20
Organism	Tribolium castaneum(red flour beetle)
Product	60 kDa heat shock protein, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450165.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_966537.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCGTT TACCGTCGAC CATGCGATCA GTCGCCCTCA GCAAAGCCAA CAGATTATCC 
CAAATCCAGC GTTGGTACGC CAAAGACGTC CGATTCGGCC CCGAAGTCCG GGCCCTGATG 
CTCCAAGGCG TCGACGTTCT TGCAGACGCT GTGGCGGTCA CCATGGGCCC TAAGGGCCGT 
AACGTGATCA TCGAACAGTC GTGGGGCTCC CCAAAGATCA CCAAAGACGG CGTCACCGTC 
GCCAAAGGCG TGGAACTCAA AGACAAGTTC CAGAACATCG GCGCCCGCTT AGTACAGGAC 
GTTGCCAACA ACACCAACGA GGAGGCAGGG GACGGAACCA CTACTGCTAC AGTATTGGCC 
CGCTCTATTG CTAAAGAGGG TTTCGAAAAT CTTGGCAAGG GCGCTAATCC AGTCGAAATT 
CGCAAAGGCA TAATGCTAGC GGTTGAGAAG ATCACGGAAA CGTTGAAAAC TCTCTCAAAG 
CCCGTCACCA CACCTGAGGA GATATGCCAG GTGGCCACGA TTTCGGCTAA TGGGGATCAG 
TCGGTTGGTA ATTTAATTGC CGATGCCATG AAGAAGGTGG GCAAAGAAGG CGTGATTACG 
GTCAAAGATG GGAAAACTCT GCACGATGAG CTTGAAGTTA TTGAAGGGTT CAAGTTTGAC 
CGCGGATACA TTTCCCCATA CTTTGTCAAT ACCTCAAAAG GTGCAAAAGT GGAATACCAG 
GATGCTCTAA TCCTCCTCAG CGAAAAGAAA ATCTCATCGG TTCAAAGCAT TGTCCCCGCC 
TTGGAGCTGG CAAACATGCA AAAGAAACCC TTAATTATCG TTGCTGAAGA CATAGACGGC 
GAAGCTTTGA CCACACTAGT CGTCAACAGA CTGCGTATTG GTCTTCAAGT GGCGGCTGTA 
AAAGCCCCAG GTTTTGGCGA CAACCGCAAA GCCACCCTCC AAGATATGGC CATCGCCACT 
GGAGGTATCG TTTTTGGAGA CGAAGCCAAT ATTGTCAAAC TTGAAGACGT CCAACTGTCC 
GATCTCGGCC AAGTTGGCGA AATTGTCATC ACTAAGGATG ATACACTTAT CTTGAAAGGG 
AAAGGCAAAA AAGACGATAT CTCTAAACGC GCTGAGCAAA TTAAAGATCA GATTGAAAAC 
ACCACTTCTG AATACGAGAA GGAGAAACTT CAGGAACGTT TGGCGAGATT GGCTTCAGGA 
GTGGCTTTGT TGAAAGTGGG AGGCAGTAGC GAGGTTGAAG TTAACGAGAA AAAAGACAGA 
GTGACTGATG CTTTGAATGC GACTAGAGCA GCTGTTGAGG AAGGAATTGT TCCTGGAGGT 
GGTACTGCCT TGCTTAGGTG CTCCGGCAGT TTGGATGGGC TCAAGCCGGG AAACAATGAC 
CAAGCTATTG GAATCGAAAT TGTGAAGAGA GCTTTGAAAG TACCTTGCAT GACCATCGCC 
AAGAACGCTG GCGTAGATGG CGCGACTGTT GTTGCCAAAA TCGAGCAACA ACAAGGTGAC 
TACGGTTACG ATGCTCTTAA CAACGAATAT GTCAATATGT TTGAAAGAGG AATCATTGAT 
CCTACGAAGG TTGTGCGCAC AGCCCTTATT GACGCGTCTG GTGTAGCGTC ACTTTTGACC 
ACCGCCGAGG CAGTAATCAC CGAAATACCA AAGGAAGAAC CGCCAATTCC AAGCGGTGGA 
ATGGGTGGTA TGGGAGGTAT GGGTGGAATG GGCGGAATGA TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_971630.1
CDS	113..1837
Translation	MYRLPSTMRSVALSKANRLSQIQRWYAKDVRFGPEVRALMLQGVDVLADAVAVTMGPKGRNVIIEQSWGSPKITKDGVTVAKGVELKDKFQNIGARLVQDVANNTNEEAGDGTTTATVLARSIAKEGFENLGKGANPVEIRKGIMLAVEKITETLKTLSKPVTTPEEICQVATISANGDQSVGNLIADAMKKVGKEGVITVKDGKTLHDELEVIEGFKFDRGYISPYFVNTSKGAKVEYQDALILLSEKKISSVQSIVPALELANMQKKPLIIVAEDIDGEALTTLVVNRLRIGLQVAAVKAPGFGDNRKATLQDMAIATGGIVFGDEANIVKLEDVQLSDLGQVGEIVITKDDTLILKGKGKKDDISKRAEQIKDQIENTTSEYEKEKLQERLARLASGVALLKVGGSSEVEVNEKKDRVTDALNATRAAVEEGIVPGGGTALLRCSGSLDGLKPGNNDQAIGIEIVKRALKVPCMTIAKNAGVDGATVVAKIEQQQGDYGYDALNNEYVNMFERGIIDPTKVVRTALIDASGVASLLTTAEAVITEIPKEEPPIPSGGMGGMGGMGGMGGMM

Target ORF information:

RefSeq Version	XM_966537.4
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum 60 kDa heat shock protein, mitochondrial (LOC660294), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_966537.4

ORF Insert Sequence:

ATGTATCGTT TACCGTCGAC CATGCGATCA GTCGCCCTCA GCAAAGCCAA CAGATTATCC 
CAAATCCAGC GTTGGTACGC CAAAGACGTC CGATTCGGCC CCGAAGTCCG GGCCCTGATG 
CTCCAAGGCG TCGACGTTCT TGCAGACGCT GTGGCGGTCA CCATGGGCCC TAAGGGCCGT 
AACGTGATCA TCGAACAGTC GTGGGGCTCC CCAAAGATCA CCAAAGACGG CGTCACCGTC 
GCCAAAGGCG TGGAACTCAA AGACAAGTTC CAGAACATCG GCGCCCGCTT AGTACAGGAC 
GTTGCCAACA ACACCAACGA GGAGGCAGGG GACGGAACCA CTACTGCTAC AGTATTGGCC 
CGCTCTATTG CTAAAGAGGG TTTCGAAAAT CTTGGCAAGG GCGCTAATCC AGTCGAAATT 
CGCAAAGGCA TAATGCTAGC GGTTGAGAAG ATCACGGAAA CGTTGAAAAC TCTCTCAAAG 
CCCGTCACCA CACCTGAGGA GATATGCCAG GTGGCCACGA TTTCGGCTAA TGGGGATCAG 
TCGGTTGGTA ATTTAATTGC CGATGCCATG AAGAAGGTGG GCAAAGAAGG CGTGATTACG 
GTCAAAGATG GGAAAACTCT GCACGATGAG CTTGAAGTTA TTGAAGGGTT CAAGTTTGAC 
CGCGGATACA TTTCCCCATA CTTTGTCAAT ACCTCAAAAG GTGCAAAAGT GGAATACCAG 
GATGCTCTAA TCCTCCTCAG CGAAAAGAAA ATCTCATCGG TTCAAAGCAT TGTCCCCGCC 
TTGGAGCTGG CAAACATGCA AAAGAAACCC TTAATTATCG TTGCTGAAGA CATAGACGGC 
GAAGCTTTGA CCACACTAGT CGTCAACAGA CTGCGTATTG GTCTTCAAGT GGCGGCTGTA 
AAAGCCCCAG GTTTTGGCGA CAACCGCAAA GCCACCCTCC AAGATATGGC CATCGCCACT 
GGAGGTATCG TTTTTGGAGA CGAAGCCAAT ATTGTCAAAC TTGAAGACGT CCAACTGTCC 
GATCTCGGCC AAGTTGGCGA AATTGTCATC ACTAAGGATG ATACACTTAT CTTGAAAGGG 
AAAGGCAAAA AAGACGATAT CTCTAAACGC GCTGAGCAAA TTAAAGATCA GATTGAAAAC 
ACCACTTCTG AATACGAGAA GGAGAAACTT CAGGAACGTT TGGCGAGATT GGCTTCAGGA 
GTGGCTTTGT TGAAAGTGGG AGGCAGTAGC GAGGTTGAAG TTAACGAGAA AAAAGACAGA 
GTGACTGATG CTTTGAATGC GACTAGAGCA GCTGTTGAGG AAGGAATTGT TCCTGGAGGT 
GGTACTGCCT TGCTTAGGTG CTCCGGCAGT TTGGATGGGC TCAAGCCGGG AAACAATGAC 
CAAGCTATTG GAATCGAAAT TGTGAAGAGA GCTTTGAAAG TACCTTGCAT GACCATCGCC 
AAGAACGCTG GCGTAGATGG CGCGACTGTT GTTGCCAAAA TCGAGCAACA ACAAGGTGAC 
TACGGTTACG ATGCTCTTAA CAACGAATAT GTCAATATGT TTGAAAGAGG AATCATTGAT 
CCTACGAAGG TTGTGCGCAC AGCCCTTATT GACGCGTCTG GTGTAGCGTC ACTTTTGACC 
ACCGCCGAGG CAGTAATCAC CGAAATACCA AAGGAAGAAC CGCCAATTCC AAGCGGTGGA 
ATGGGTGGTA TGGGAGGTAT GGGTGGAATG GGCGGAATGA TGTAA