LOC663145 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC663145
Entrez Gene ID	663145
Full Name	cathepsin B precursor
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_969205.3	XP_974298.1	cathepsin B

Pathways from BioSystems
Gene Ontology

The following LOC663145 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC663145 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTg03097	XM_969205.3 Latest version!	Tribolium castaneum cathepsin B precursor (LOC663145), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC663145 ( XM_969205.3 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_974298.1 Tribolium castaneum cathepsin B precursor (LOC663145), mRNA.

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CloneID	OTg03097
Clone ID Related Accession (Same CDS sequence)	XM_969205.3
Accession Version	XM_969205.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-20
Organism	Tribolium castaneum(red flour beetle)
Product	cathepsin B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450144.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_969205.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTGCG TCTTGCTTTG TGCTGTTGTT CTCGCAACCA TCGCCCTCTC TTACGGAGGG 
CTGAACCCGC ACCCCCTTTC AGACGAATTC ATCAACGCCA TCAACAGCAA GAAAACCACA 
TGGAAAGCTG GAAGAAACTT TGACATCCAC ACCCCCCTTG CCAACATCAA AAAACTTCTT 
GGAGTTCTTC CCAAGAAGGC AAATGCCCGC CAACTAGAAC TGAAAGTGCA CTCAGTCGAC 
GTTAACGCCA TTCCGGAGTC TTTCGACGCC AGGGAAGCCT GGCCAGAGTG CGCTTCCATC 
ATTGGCGATA TCAGAGATCA AGCCTCTTGT GGTTCCTGCT GGGCCTTTGG CGCCGCCGAA 
GCCATGAGCG ACCGCATCTG CATTCACTCA AACGCCACCG TCAAAGTCAG TATCTCCACC 
GAGGACTTGA ACACTTGCTG CTACGAATGC GGAGACGGTT GCAACGGTGG ATGGCCTGCC 
GAAGCTTGGG CTTACTGGGC AGAAACCGGA ATTGTAACTG GTGGCAAATA CGAGACTAAA 
GACGGTTGCA AAGCTTACAC CGTCCCCCCT TGCGAACACC ACACCGAAGG CGACTTGCCT 
GCGTGTGGAG ACATTGTCCC AACCCCCCAA TGCAAGAAAG AGTGTGACGC CGGTGTTGAT 
ATCGAGTACA AGTCTGATCT GAGAAAAGGG TCTGCGTACC AAACCTCATC TGATGAATCT 
CAAATTCAAA CCGAAATTAT GACAAACGGA CCTGTTGAGG CCGATTTTGA CGTGTATGAA 
GATTTCCTCA ACTACAAAAG TGGGGTCTAC CAACAAACCA CCGGTAATTA TGCCGGTGGA 
CACGCTATTA AGATTTTGGG ATGGGGTGTG GAAGACGGTA CGCCGTACTG GTTGGCCGCT 
AACTCGTGGA ATGAGGACTG GGGCGACAAG GGATACTTCA AAATTTTGAG AGGACAAAAC 
GAATGTGGGA TTGAGAGCGA TATTATCGGC GGAATTCCAG TTGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_974298.1
CDS	108..1115
Translation	MKCVLLCAVVLATIALSYGGLNPHPLSDEFINAINSKKTTWKAGRNFDIHTPLANIKKLLGVLPKKANARQLELKVHSVDVNAIPESFDAREAWPECASIIGDIRDQASCGSCWAFGAAEAMSDRICIHSNATVKVSISTEDLNTCCYECGDGCNGGWPAEAWAYWAETGIVTGGKYETKDGCKAYTVPPCEHHTEGDLPACGDIVPTPQCKKECDAGVDIEYKSDLRKGSAYQTSSDESQIQTEIMTNGPVEADFDVYEDFLNYKSGVYQQTTGNYAGGHAIKILGWGVEDGTPYWLAANSWNEDWGDKGYFKILRGQNECGIESDIIGGIPVV

Target ORF information:

RefSeq Version	XM_969205.3
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum cathepsin B precursor (LOC663145), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_969205.3

ORF Insert Sequence:

ATGAAGTGCG TCTTGCTTTG TGCTGTTGTT CTCGCAACCA TCGCCCTCTC TTACGGAGGG 
CTGAACCCGC ACCCCCTTTC AGACGAATTC ATCAACGCCA TCAACAGCAA GAAAACCACA 
TGGAAAGCTG GAAGAAACTT TGACATCCAC ACCCCCCTTG CCAACATCAA AAAACTTCTT 
GGAGTTCTTC CCAAGAAGGC AAATGCCCGC CAACTAGAAC TGAAAGTGCA CTCAGTCGAC 
GTTAACGCCA TTCCGGAGTC TTTCGACGCC AGGGAAGCCT GGCCAGAGTG CGCTTCCATC 
ATTGGCGATA TCAGAGATCA AGCCTCTTGT GGTTCCTGCT GGGCCTTTGG CGCCGCCGAA 
GCCATGAGCG ACCGCATCTG CATTCACTCA AACGCCACCG TCAAAGTCAG TATCTCCACC 
GAGGACTTGA ACACTTGCTG CTACGAATGC GGAGACGGTT GCAACGGTGG ATGGCCTGCC 
GAAGCTTGGG CTTACTGGGC AGAAACCGGA ATTGTAACTG GTGGCAAATA CGAGACTAAA 
GACGGTTGCA AAGCTTACAC CGTCCCCCCT TGCGAACACC ACACCGAAGG CGACTTGCCT 
GCGTGTGGAG ACATTGTCCC AACCCCCCAA TGCAAGAAAG AGTGTGACGC CGGTGTTGAT 
ATCGAGTACA AGTCTGATCT GAGAAAAGGG TCTGCGTACC AAACCTCATC TGATGAATCT 
CAAATTCAAA CCGAAATTAT GACAAACGGA CCTGTTGAGG CCGATTTTGA CGTGTATGAA 
GATTTCCTCA ACTACAAAAG TGGGGTCTAC CAACAAACCA CCGGTAATTA TGCCGGTGGA 
CACGCTATTA AGATTTTGGG ATGGGGTGTG GAAGACGGTA CGCCGTACTG GTTGGCCGCT 
AACTCGTGGA ATGAGGACTG GGGCGACAAG GGATACTTCA AAATTTTGAG AGGACAAAAC 
GAATGTGGGA TTGAGAGCGA TATTATCGGC GGAATTCCAG TTGTATAA