insr cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	insr
Entrez Gene ID	100492718
Full Name	insulin receptor
Synonyms	XTK1,XTK1a,XTK1b,Xe-InsR,cd220,hhf5
General protein information	Preferred Names insulin receptor Names insulin receptor
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012953588.2	XP_012809042.1	insulin receptor isoform X2
XM_002934379.5	XP_002934425.2	insulin receptor isoform X1
XM_012953588.3	XP_012809042.1	insulin receptor isoform X2
XM_002934379.4	XP_002934425.2	insulin receptor isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	INSR	XP_002688878.3
Danio rerio (zebrafish)	insra	NP_001136144.1
Homo sapiens (human)	INSR	NP_000199.2
Danio rerio (zebrafish)	insrb	NP_001116701.1
	INSR	XP_001094337.2
Canis lupus familiaris (dog)	INSR	XP_005633279.1
Gallus gallus (chicken)	INSR	XP_001233399.3
Mus musculus (house mouse)	Insr	NP_034698.2
Pan troglodytes (chimpanzee)	INSR	XP_512324.2
Rattus norvegicus (Norway rat)	Insr	NP_058767.2
Xenopus tropicalis (tropical clawed frog)	LOC100492718	XP_002934425.2

Gene Ontology

The following insr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the insr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa04156	XM_002934379.5 Latest version!	Xenopus tropicalis insulin receptor (insr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa35450	XM_012953588.3 Latest version!	Xenopus tropicalis insulin receptor (insr), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OXa35450	XM_012953588.2	Xenopus tropicalis insulin receptor (insr), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa04156	XM_002934379.4	Xenopus tropicalis insulin receptor (insr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

insr ( XM_002934379.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934425.2 Xenopus tropicalis insulin receptor (insr), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa04156
Clone ID Related Accession (Same CDS sequence)	XM_002934379.5 , XM_002934379.4
Accession Version	XM_002934379.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	insulin receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934379.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACAGG GTGTATTGTG GGGTGAGGGG CACCCCAATA ATAACCCCAA GAGCAAGGTG 
GGCTGGAAGT CCATTGCGGG AATATTCATC ATTTTTATGC TCATTCTGTG CGACCAAAAC 
GACGGGAAAA TTTGCCCCAG TATGGATATC CGGAATAACA TTAGTCAGTT CAGTATGCTT 
GAGGACTGTA CAGTAATTGA AGGTCACCTT CAGATCTTGC TTATGTTCAC CAGCAAACCA 
GAGAACTTCC GAGGGTTGAG ATTTCCCAAG CTCACCATGA TCACAGATTA CCTCCTGCTT 
TTCCGGGTCT ATGGCTTAGA AAGTCTAAAG GACCTTTTTC CCAACCTCAC AGTTATCCGT 
GGTACTCACC TCTTTTTCAA CTATGCCCTA GTCATCTTTG AGATGGTCCA CCTGAAGGAG 
ATTGGGCTCT ACAACCTAAT GAACATTACT AGGGGGTCTG TTAGAATTGA GAAAAATAAT 
GAACTTTGTT ATCTTTCTAC GATTGATTGG TCCATAATTC TTGACTCTGT GGAGGATAAC 
TACATTGAAC TCAACAGGGA CAATAAAGAG GAGTGTGGAG ATGTTTGTCC TGGTGCAGGA 
AAAGGCAAGA GCAAATGCAA ACAGATAGTA GTCAATGGAG CATTCATTGA GCGCTGCTGG 
ACACAGGACC ACTGTCAAAC AGTGTGCCCT TCAGAATGCA AGGGAAGTGG TTGTTTGCCA 
GATGGACAGT GCTGCCACTC AGAGTGCCTT GGAAGCTGCA GAAAGCCTAA TGATCCAACT 
GAGTGTACCG CCTGCCGGCA TTACCAGTAT CAGGGTGTGT GTATCAGTGC ATGTCCCGAG 
GGGTCTTACC GGTTCCAGGG TTGGCGGTGC ATTGACTTCA ATACCTGTCA AGAAATGCAC 
AACCGGTGCC CAAATAAGGA AAATTCCTGT CCTCCTTATG TCATCCACAA GGGAGAGTGC 
ATGCCAGATT GCCCATCAGG CTATATTGCC AACTCAACTA CCCGTACTTG TACACCATGC 
GCAGGACCTT GCCCCAAAGT TTGCACCATC TACCAAAGTG TCAAGACCAT AGACTCTGTG 
ACATCTGCTC AAGAATTAAG AGGGTGCACA GTGGTCAACG GTAGTCTTAT CATCAACATC 
CGTGGAGGCA ATAATATTGC AACAGAATTA GAAGCTAACC TGGGCCTCAT TGAGGAGATT 
TCTGGATATC TTAAGATCAG AAGATCATAT GCATTGGTGT CTTTGTCATT CTTCCGCAAG 
CTGCGTTTAA TTAGGGGAGA AGTGTTGGAA GCAGGTAACT ATTCCTTCTA TGCTCTGGAT 
AATCCCAGTC TACGCCAGCT GTGGGACTGG CATAAGCACA ACCTGACCAT CATACATGGC 
AAGCTCTTCT TCCATCACAA CCCACGACTA TGTCTAGCGG AGATTCACCA AATGGAGGAG 
GTTACAGGAA CAAAGGGCCG GCAAGACAAG AATGATATAT CTACAAAGAC CAATGGAGAT 
CAGGCATCTT GTGAGGACAA TTTACTGAAC TTTAACTTTA TAAGGACATC GCATGATATG 
GTGCTGTTAA AGTGGGATCC TTACTGGCCC CCTGATTACA GAGACCTCTT GGGCTTCATG 
GTGCATTATA AGGAAGCACC CTACCAGAAT GTCACAGAAT TTGATGGTCA GGATGCTTGT 
GGTTCCAATA GTTGGACAGT GGTAGATATG GATGCCCCAG AACGAAGTGC TGATGGCAAG 
ACTCTATCTC CGGGATGTTT GCTCCGTAAC CTGAAACCAT GGACTCAGTA TGCAGTGTTT 
GTTAAAACAC TGGTATCCGG ATCAGATGAA GGAAGAACTT ATGGTGCAAA AAGCAAAATT 
ATCTATATTC GTACCAATGA AACCATCCCC TCTGTTCCTC TGGATCCTTT CTCTGTGTCC 
AACTCAACCT CTCAGATTAT TCTAAAATGG AAACCACCTT CTGAGCCCAA TGGAAATGTC 
ACTCACTACC TTGTGTACTG GCAAGAACAA CCTGAAGACA GTGAACTTTA TGAGCTGGAT 
TACTGCAATA AAGGTCTTAA GTTGCCTTCC AGAACTTGGA CTCCTCCCTC GGATATTGAG 
GAAAATGGAA ATATAAATCA GACAGACCAA CCTTCATCCA AGTGCTGTCC CTGTCCCAAA 
ACTGATTTCC AAATCCAGAA AGAACAGGAT GAATCAGCAT TCAGAAAAAC ATTTGAAAAT 
TATCTACATA ATGAAGTTTT TATACCTAGA CCTGTTCGAA AACGGCGTGA ACTATTTGGA 
GTAGCCAATG GAACTCTGCC AGACCCAGTG ACAGCCCCTT CTCTCTTCAA TGCCTCCTCC 
ACCCGTGCTC CGGATGAAAC AGAGCCAAAG ATATATTCAC AGAAAGTTTG GTTTAAGGAG 
TCTCTAATGG TCACTGGCTT AAAGCATTTT ACTGGTTACC GCATTGAGAT TCATGCCTGC 
AACCACGAAC TCTCCATGGG ATGTAGCGTG GCAGCATATG TTAGTGCAAG GACAATGCCA 
GAGGCCACTG CCGACAATGT TGTGGGACCC ATCACCTATG AGTATATTGA ACCTAATATA 
ATCCATTTAA AATGGCAAGA ACCAAAAGAT CCAAATGGGC TAATAGTTTT GTATGAGGTG 
CATTACTCCC GAGTTGGGGG CACAGAGGAA GTGATTAGCT GCGTGTCACA GAAGCAGTAT 
AGCACAGACA AAGGGGCAAA ACTCAGGGTA TTAACCCCAG GGAATTACAG TGTAAAGATC 
CGTGCAACCT CCCTGGCAGG CAATGGATCA TGGACAGAAC CAGCCTACTT CCAAGTGCCA 
GACCACCCTC ACAGCAGCAT AGTGAAAATC GTCACTGGTC CAGTAATTGC AGTCATTCTT 
CTGCTTTTTG TCCTGGTTTA CTGTGTTATT CAGAAGAAAA AAGATGCAGA AGGCCCAGTT 
GGACCACTCT ACGCCTCCTC TAATCCTGAA TATCTTAGTG CAAGTGAAGT CTACATCCCT 
GACGAATGGG AGGTACCACG TGATAAAATC AGCTTACTGC GAGAATTGGG CCAGGGCTCA 
TTTGGAATGG TGTACGAGGG TATTGCCAAA GATATTATCA AGGGAGAGCC AGAAGTTCGT 
GTTGCTGTGA AAACTGTGAA TGAGTCTGCA AGCCTTCGAG AGAGGATAGA ATTCCTAAAT 
GAGGCTTCTG TCATGAAGGC ATTTAGCTGC CACCATGTGG TCCGTCTGCT TGGTGTGGTG 
TCCAAAGGTC AGCCAACACT TGTCATCATG GAGTTAATGA CGCATGGGGA TCTCAAAAGT 
TACCTGCGCT CACTGCGACC TGATGCAGAG AATAACCCAG GACGTCCTGC TCCTACCCTT 
AAAGAAATGA TCCAGATGGC AGCGGAAATT TCAGATGGCA TGGCATATCT CAATGCTAAA 
AAGTTTGTAC ACAGGGATCT GGCTGCTAGG AACTGCATGG TTGCTGAAGA TTATACTGTG 
AAAATTGGAG ATTTTGGAAT GACACGAGAT ATCTATGAGA CAGATTATTA CCGAAAAGGG 
GGAAAGGGGC TGCTGCCTGT TAGATGGATG TCCCCTGAAT CACTGAAAGA TGGAGTGTTC 
ACAACTTTCT CAGATGTCTG GTCCTTTGGA GTTGTTTTAT GGGAAATAAC GAGTTTGGCA 
GAACAGCCAT ATCAGGGACT TTCTAATGAG CAGGTCCTGA AGTTTGTCAT GGATGGAGGT 
TCACTTGACC AACCGGAGAA TTGCCCTCCA AGGCTGCATA GTCTGATGCA GATGTGCTGG 
CAGTATAACC CCAAGATGCG GCCTACCTTC TTGGAGATTA TCGACATGTT AAAAGATGAC 
CTGCATCTTA GTTTTCAGGA AGTCTCATTC TACTACAGTG ACGAGAACAA GCCACCTGAA 
ACAGATGATT TGGAAATTGA TTTTGAAAAT ATGGAGAGCA CACCCCTGGA CCCTTCTTCC 
TGTTCTTTAC GGGATCAGTC GAATCGGGGG AACATTTATG AAGAACACAT CCCCTACACC 
CATATGAATG GTGGCAGAAA AAATGGACGT ATACTGTCTT TGCCACGGTC CAGCCCATCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934425.2
CDS	1017..5099
Translation	MGQGVLWGEGHPNNNPKSKVGWKSIAGIFIIFMLILCDQNDGKICPSMDIRNNISQFSMLEDCTVIEGHLQILLMFTSKPENFRGLRFPKLTMITDYLLLFRVYGLESLKDLFPNLTVIRGTHLFFNYALVIFEMVHLKEIGLYNLMNITRGSVRIEKNNELCYLSTIDWSIILDSVEDNYIELNRDNKEECGDVCPGAGKGKSKCKQIVVNGAFIERCWTQDHCQTVCPSECKGSGCLPDGQCCHSECLGSCRKPNDPTECTACRHYQYQGVCISACPEGSYRFQGWRCIDFNTCQEMHNRCPNKENSCPPYVIHKGECMPDCPSGYIANSTTRTCTPCAGPCPKVCTIYQSVKTIDSVTSAQELRGCTVVNGSLIINIRGGNNIATELEANLGLIEEISGYLKIRRSYALVSLSFFRKLRLIRGEVLEAGNYSFYALDNPSLRQLWDWHKHNLTIIHGKLFFHHNPRLCLAEIHQMEEVTGTKGRQDKNDISTKTNGDQASCEDNLLNFNFIRTSHDMVLLKWDPYWPPDYRDLLGFMVHYKEAPYQNVTEFDGQDACGSNSWTVVDMDAPERSADGKTLSPGCLLRNLKPWTQYAVFVKTLVSGSDEGRTYGAKSKIIYIRTNETIPSVPLDPFSVSNSTSQIILKWKPPSEPNGNVTHYLVYWQEQPEDSELYELDYCNKGLKLPSRTWTPPSDIEENGNINQTDQPSSKCCPCPKTDFQIQKEQDESAFRKTFENYLHNEVFIPRPVRKRRELFGVANGTLPDPVTAPSLFNASSTRAPDETEPKIYSQKVWFKESLMVTGLKHFTGYRIEIHACNHELSMGCSVAAYVSARTMPEATADNVVGPITYEYIEPNIIHLKWQEPKDPNGLIVLYEVHYSRVGGTEEVISCVSQKQYSTDKGAKLRVLTPGNYSVKIRATSLAGNGSWTEPAYFQVPDHPHSSIVKIVTGPVIAVILLLFVLVYCVIQKKKDAEGPVGPLYASSNPEYLSASEVYIPDEWEVPRDKISLLRELGQGSFGMVYEGIAKDIIKGEPEVRVAVKTVNESASLRERIEFLNEASVMKAFSCHHVVRLLGVVSKGQPTLVIMELMTHGDLKSYLRSLRPDAENNPGRPAPTLKEMIQMAAEISDGMAYLNAKKFVHRDLAARNCMVAEDYTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMSPESLKDGVFTTFSDVWSFGVVLWEITSLAEQPYQGLSNEQVLKFVMDGGSLDQPENCPPRLHSLMQMCWQYNPKMRPTFLEIIDMLKDDLHLSFQEVSFYYSDENKPPETDDLEIDFENMESTPLDPSSCSLRDQSNRGNIYEEHIPYTHMNGGRKNGRILSLPRSSPS

Target ORF information:

RefSeq Version	XM_002934379.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis insulin receptor (insr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934379.5

ORF Insert Sequence:

ATGGGACAGG GTGTATTGTG GGGTGAGGGG CACCCCAATA ATAACCCCAA GAGCAAGGTG 
GGCTGGAAGT CCATTGCGGG AATATTCATC ATTTTTATGC TCATTCTGTG CGACCAAAAC 
GACGGGAAAA TTTGCCCCAG TATGGATATC CGGAATAACA TTAGTCAGTT CAGTATGCTT 
GAGGACTGTA CAGTAATTGA AGGTCACCTT CAGATCTTGC TTATGTTCAC CAGCAAACCA 
GAGAACTTCC GAGGGTTGAG ATTTCCCAAG CTCACCATGA TCACAGATTA CCTCCTGCTT 
TTCCGGGTCT ATGGCTTAGA AAGTCTAAAG GACCTTTTTC CCAACCTCAC AGTTATCCGT 
GGTACTCACC TCTTTTTCAA CTATGCCCTA GTCATCTTTG AGATGGTCCA CCTGAAGGAG 
ATTGGGCTCT ACAACCTAAT GAACATTACT AGGGGGTCTG TTAGAATTGA GAAAAATAAT 
GAACTTTGTT ATCTTTCTAC GATTGATTGG TCCATAATTC TTGACTCTGT GGAGGATAAC 
TACATTGAAC TCAACAGGGA CAATAAAGAG GAGTGTGGAG ATGTTTGTCC TGGTGCAGGA 
AAAGGCAAGA GCAAATGCAA ACAGATAGTA GTCAATGGAG CATTCATTGA GCGCTGCTGG 
ACACAGGACC ACTGTCAAAC AGTGTGCCCT TCAGAATGCA AGGGAAGTGG TTGTTTGCCA 
GATGGACAGT GCTGCCACTC AGAGTGCCTT GGAAGCTGCA GAAAGCCTAA TGATCCAACT 
GAGTGTACCG CCTGCCGGCA TTACCAGTAT CAGGGTGTGT GTATCAGTGC ATGTCCCGAG 
GGGTCTTACC GGTTCCAGGG TTGGCGGTGC ATTGACTTCA ATACCTGTCA AGAAATGCAC 
AACCGGTGCC CAAATAAGGA AAATTCCTGT CCTCCTTATG TCATCCACAA GGGAGAGTGC 
ATGCCAGATT GCCCATCAGG CTATATTGCC AACTCAACTA CCCGTACTTG TACACCATGC 
GCAGGACCTT GCCCCAAAGT TTGCACCATC TACCAAAGTG TCAAGACCAT AGACTCTGTG 
ACATCTGCTC AAGAATTAAG AGGGTGCACA GTGGTCAACG GTAGTCTTAT CATCAACATC 
CGTGGAGGCA ATAATATTGC AACAGAATTA GAAGCTAACC TGGGCCTCAT TGAGGAGATT 
TCTGGATATC TTAAGATCAG AAGATCATAT GCATTGGTGT CTTTGTCATT CTTCCGCAAG 
CTGCGTTTAA TTAGGGGAGA AGTGTTGGAA GCAGGTAACT ATTCCTTCTA TGCTCTGGAT 
AATCCCAGTC TACGCCAGCT GTGGGACTGG CATAAGCACA ACCTGACCAT CATACATGGC 
AAGCTCTTCT TCCATCACAA CCCACGACTA TGTCTAGCGG AGATTCACCA AATGGAGGAG 
GTTACAGGAA CAAAGGGCCG GCAAGACAAG AATGATATAT CTACAAAGAC CAATGGAGAT 
CAGGCATCTT GTGAGGACAA TTTACTGAAC TTTAACTTTA TAAGGACATC GCATGATATG 
GTGCTGTTAA AGTGGGATCC TTACTGGCCC CCTGATTACA GAGACCTCTT GGGCTTCATG 
GTGCATTATA AGGAAGCACC CTACCAGAAT GTCACAGAAT TTGATGGTCA GGATGCTTGT 
GGTTCCAATA GTTGGACAGT GGTAGATATG GATGCCCCAG AACGAAGTGC TGATGGCAAG 
ACTCTATCTC CGGGATGTTT GCTCCGTAAC CTGAAACCAT GGACTCAGTA TGCAGTGTTT 
GTTAAAACAC TGGTATCCGG ATCAGATGAA GGAAGAACTT ATGGTGCAAA AAGCAAAATT 
ATCTATATTC GTACCAATGA AACCATCCCC TCTGTTCCTC TGGATCCTTT CTCTGTGTCC 
AACTCAACCT CTCAGATTAT TCTAAAATGG AAACCACCTT CTGAGCCCAA TGGAAATGTC 
ACTCACTACC TTGTGTACTG GCAAGAACAA CCTGAAGACA GTGAACTTTA TGAGCTGGAT 
TACTGCAATA AAGGTCTTAA GTTGCCTTCC AGAACTTGGA CTCCTCCCTC GGATATTGAG 
GAAAATGGAA ATATAAATCA GACAGACCAA CCTTCATCCA AGTGCTGTCC CTGTCCCAAA 
ACTGATTTCC AAATCCAGAA AGAACAGGAT GAATCAGCAT TCAGAAAAAC ATTTGAAAAT 
TATCTACATA ATGAAGTTTT TATACCTAGA CCTGTTCGAA AACGGCGTGA ACTATTTGGA 
GTAGCCAATG GAACTCTGCC AGACCCAGTG ACAGCCCCTT CTCTCTTCAA TGCCTCCTCC 
ACCCGTGCTC CGGATGAAAC AGAGCCAAAG ATATATTCAC AGAAAGTTTG GTTTAAGGAG 
TCTCTAATGG TCACTGGCTT AAAGCATTTT ACTGGTTACC GCATTGAGAT TCATGCCTGC 
AACCACGAAC TCTCCATGGG ATGTAGCGTG GCAGCATATG TTAGTGCAAG GACAATGCCA 
GAGGCCACTG CCGACAATGT TGTGGGACCC ATCACCTATG AGTATATTGA ACCTAATATA 
ATCCATTTAA AATGGCAAGA ACCAAAAGAT CCAAATGGGC TAATAGTTTT GTATGAGGTG 
CATTACTCCC GAGTTGGGGG CACAGAGGAA GTGATTAGCT GCGTGTCACA GAAGCAGTAT 
AGCACAGACA AAGGGGCAAA ACTCAGGGTA TTAACCCCAG GGAATTACAG TGTAAAGATC 
CGTGCAACCT CCCTGGCAGG CAATGGATCA TGGACAGAAC CAGCCTACTT CCAAGTGCCA 
GACCACCCTC ACAGCAGCAT AGTGAAAATC GTCACTGGTC CAGTAATTGC AGTCATTCTT 
CTGCTTTTTG TCCTGGTTTA CTGTGTTATT CAGAAGAAAA AAGATGCAGA AGGCCCAGTT 
GGACCACTCT ACGCCTCCTC TAATCCTGAA TATCTTAGTG CAAGTGAAGT CTACATCCCT 
GACGAATGGG AGGTACCACG TGATAAAATC AGCTTACTGC GAGAATTGGG CCAGGGCTCA 
TTTGGAATGG TGTACGAGGG TATTGCCAAA GATATTATCA AGGGAGAGCC AGAAGTTCGT 
GTTGCTGTGA AAACTGTGAA TGAGTCTGCA AGCCTTCGAG AGAGGATAGA ATTCCTAAAT 
GAGGCTTCTG TCATGAAGGC ATTTAGCTGC CACCATGTGG TCCGTCTGCT TGGTGTGGTG 
TCCAAAGGTC AGCCAACACT TGTCATCATG GAGTTAATGA CGCATGGGGA TCTCAAAAGT 
TACCTGCGCT CACTGCGACC TGATGCAGAG AATAACCCAG GACGTCCTGC TCCTACCCTT 
AAAGAAATGA TCCAGATGGC AGCGGAAATT TCAGATGGCA TGGCATATCT CAATGCTAAA 
AAGTTTGTAC ACAGGGATCT GGCTGCTAGG AACTGCATGG TTGCTGAAGA TTATACTGTG 
AAAATTGGAG ATTTTGGAAT GACACGAGAT ATCTATGAGA CAGATTATTA CCGAAAAGGG 
GGAAAGGGGC TGCTGCCTGT TAGATGGATG TCCCCTGAAT CACTGAAAGA TGGAGTGTTC 
ACAACTTTCT CAGATGTCTG GTCCTTTGGA GTTGTTTTAT GGGAAATAAC GAGTTTGGCA 
GAACAGCCAT ATCAGGGACT TTCTAATGAG CAGGTCCTGA AGTTTGTCAT GGATGGAGGT 
TCACTTGACC AACCGGAGAA TTGCCCTCCA AGGCTGCATA GTCTGATGCA GATGTGCTGG 
CAGTATAACC CCAAGATGCG GCCTACCTTC TTGGAGATTA TCGACATGTT AAAAGATGAC 
CTGCATCTTA GTTTTCAGGA AGTCTCATTC TACTACAGTG ACGAGAACAA GCCACCTGAA 
ACAGATGATT TGGAAATTGA TTTTGAAAAT ATGGAGAGCA CACCCCTGGA CCCTTCTTCC 
TGTTCTTTAC GGGATCAGTC GAATCGGGGG AACATTTATG AAGAACACAT CCCCTACACC 
CATATGAATG GTGGCAGAAA AAATGGACGT ATACTGTCTT TGCCACGGTC CAGCCCATCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

insr ( XM_012953588.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809042.1 Xenopus tropicalis insulin receptor (insr), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa35450
Clone ID Related Accession (Same CDS sequence)	XM_012953588.2 , XM_012953588.3
Accession Version	XM_012953588.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	insulin receptor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012953588.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATCC GGAATAACAT TAGTCAGTTC AGTATGCTTG AGGACTGTAC AGTAATTGAA 
GGTCACCTTC AGATCTTGCT TATGTTCACC AGCAAACCAG AGAACTTCCG AGGGTTGAGA 
TTTCCCAAGC TCACCATGAT CACAGATTAC CTCCTGCTTT TCCGGGTCTA TGGCTTAGAA 
AGTCTAAAGG ACCTTTTTCC CAACCTCACA GTTATCCGTG GTACTCACCT CTTTTTCAAC 
TATGCCCTAG TCATCTTTGA GATGGTCCAC CTGAAGGAGA TTGGGCTCTA CAACCTAATG 
AACATTACTA GGGGGTCTGT TAGAATTGAG AAAAATAATG AACTTTGTTA TCTTTCTACG 
ATTGATTGGT CCATAATTCT TGACTCTGTG GAGGATAACT ACATTGAACT CAACAGGGAC 
AATAAAGAGG AGTGTGGAGA TGTTTGTCCT GGTGCAGGAA AAGGCAAGAG CAAATGCAAA 
CAGATAGTAG TCAATGGAGC ATTCATTGAG CGCTGCTGGA CACAGGACCA CTGTCAAACA 
GTGTGCCCTT CAGAATGCAA GGGAAGTGGT TGTTTGCCAG ATGGACAGTG CTGCCACTCA 
GAGTGCCTTG GAAGCTGCAG AAAGCCTAAT GATCCAACTG AGTGTACCGC CTGCCGGCAT 
TACCAGTATC AGGGTGTGTG TATCAGTGCA TGTCCCGAGG GGTCTTACCG GTTCCAGGGT 
TGGCGGTGCA TTGACTTCAA TACCTGTCAA GAAATGCACA ACCGGTGCCC AAATAAGGAA 
AATTCCTGTC CTCCTTATGT CATCCACAAG GGAGAGTGCA TGCCAGATTG CCCATCAGGC 
TATATTGCCA ACTCAACTAC CCGTACTTGT ACACCATGCG CAGGACCTTG CCCCAAAGTT 
TGCACCATCT ACCAAAGTGT CAAGACCATA GACTCTGTGA CATCTGCTCA AGAATTAAGA 
GGGTGCACAG TGGTCAACGG TAGTCTTATC ATCAACATCC GTGGAGGCAA TAATATTGCA 
ACAGAATTAG AAGCTAACCT GGGCCTCATT GAGGAGATTT CTGGATATCT TAAGATCAGA 
AGATCATATG CATTGGTGTC TTTGTCATTC TTCCGCAAGC TGCGTTTAAT TAGGGGAGAA 
GTGTTGGAAG CAGGTAACTA TTCCTTCTAT GCTCTGGATA ATCCCAGTCT ACGCCAGCTG 
TGGGACTGGC ATAAGCACAA CCTGACCATC ATACATGGCA AGCTCTTCTT CCATCACAAC 
CCACGACTAT GTCTAGCGGA GATTCACCAA ATGGAGGAGG TTACAGGAAC AAAGGGCCGG 
CAAGACAAGA ATGATATATC TACAAAGACC AATGGAGATC AGGCATCTTG TGAGGACAAT 
TTACTGAACT TTAACTTTAT AAGGACATCG CATGATATGG TGCTGTTAAA GTGGGATCCT 
TACTGGCCCC CTGATTACAG AGACCTCTTG GGCTTCATGG TGCATTATAA GGAAGCACCC 
TACCAGAATG TCACAGAATT TGATGGTCAG GATGCTTGTG GTTCCAATAG TTGGACAGTG 
GTAGATATGG ATGCCCCAGA ACGAAGTGCT GATGGCAAGA CTCTATCTCC GGGATGTTTG 
CTCCGTAACC TGAAACCATG GACTCAGTAT GCAGTGTTTG TTAAAACACT GGTATCCGGA 
TCAGATGAAG GAAGAACTTA TGGTGCAAAA AGCAAAATTA TCTATATTCG TACCAATGAA 
ACCATCCCCT CTGTTCCTCT GGATCCTTTC TCTGTGTCCA ACTCAACCTC TCAGATTATT 
CTAAAATGGA AACCACCTTC TGAGCCCAAT GGAAATGTCA CTCACTACCT TGTGTACTGG 
CAAGAACAAC CTGAAGACAG TGAACTTTAT GAGCTGGATT ACTGCAATAA AGGTCTTAAG 
TTGCCTTCCA GAACTTGGAC TCCTCCCTCG GATATTGAGG AAAATGGAAA TATAAATCAG 
ACAGACCAAC CTTCATCCAA GTGCTGTCCC TGTCCCAAAA CTGATTTCCA AATCCAGAAA 
GAACAGGATG AATCAGCATT CAGAAAAACA TTTGAAAATT ATCTACATAA TGAAGTTTTT 
ATACCTAGAC CTGTTCGAAA ACGGCGTGAA CTATTTGGAG TAGCCAATGG AACTCTGCCA 
GACCCAGTGA CAGCCCCTTC TCTCTTCAAT GCCTCCTCCA CCCGTGCTCC GGATGAAACA 
GAGCCAAAGA TATATTCACA GAAAGTTTGG TTTAAGGAGT CTCTAATGGT CACTGGCTTA 
AAGCATTTTA CTGGTTACCG CATTGAGATT CATGCCTGCA ACCACGAACT CTCCATGGGA 
TGTAGCGTGG CAGCATATGT TAGTGCAAGG ACAATGCCAG AGGCCACTGC CGACAATGTT 
GTGGGACCCA TCACCTATGA GTATATTGAA CCTAATATAA TCCATTTAAA ATGGCAAGAA 
CCAAAAGATC CAAATGGGCT AATAGTTTTG TATGAGGTGC ATTACTCCCG AGTTGGGGGC 
ACAGAGGAAG TGATTAGCTG CGTGTCACAG AAGCAGTATA GCACAGACAA AGGGGCAAAA 
CTCAGGGTAT TAACCCCAGG GAATTACAGT GTAAAGATCC GTGCAACCTC CCTGGCAGGC 
AATGGATCAT GGACAGAACC AGCCTACTTC CAAGTGCCAG ACCACCCTCA CAGCAGCATA 
GTGAAAATCG TCACTGGTCC AGTAATTGCA GTCATTCTTC TGCTTTTTGT CCTGGTTTAC 
TGTGTTATTC AGAAGAAAAA AGATGCAGAA GGCCCAGTTG GACCACTCTA CGCCTCCTCT 
AATCCTGAAT ATCTTAGTGC AAGTGAAGTC TACATCCCTG ACGAATGGGA GGTACCACGT 
GATAAAATCA GCTTACTGCG AGAATTGGGC CAGGGCTCAT TTGGAATGGT GTACGAGGGT 
ATTGCCAAAG ATATTATCAA GGGAGAGCCA GAAGTTCGTG TTGCTGTGAA AACTGTGAAT 
GAGTCTGCAA GCCTTCGAGA GAGGATAGAA TTCCTAAATG AGGCTTCTGT CATGAAGGCA 
TTTAGCTGCC ACCATGTGGT CCGTCTGCTT GGTGTGGTGT CCAAAGGTCA GCCAACACTT 
GTCATCATGG AGTTAATGAC GCATGGGGAT CTCAAAAGTT ACCTGCGCTC ACTGCGACCT 
GATGCAGAGA ATAACCCAGG ACGTCCTGCT CCTACCCTTA AAGAAATGAT CCAGATGGCA 
GCGGAAATTT CAGATGGCAT GGCATATCTC AATGCTAAAA AGTTTGTACA CAGGGATCTG 
GCTGCTAGGA ACTGCATGGT TGCTGAAGAT TATACTGTGA AAATTGGAGA TTTTGGAATG 
ACACGAGATA TCTATGAGAC AGATTATTAC CGAAAAGGGG GAAAGGGGCT GCTGCCTGTT 
AGATGGATGT CCCCTGAATC ACTGAAAGAT GGAGTGTTCA CAACTTTCTC AGATGTCTGG 
TCCTTTGGAG TTGTTTTATG GGAAATAACG AGTTTGGCAG AACAGCCATA TCAGGGACTT 
TCTAATGAGC AGGTCCTGAA GTTTGTCATG GATGGAGGTT CACTTGACCA ACCGGAGAAT 
TGCCCTCCAA GGCTGCATAG TCTGATGCAG ATGTGCTGGC AGTATAACCC CAAGATGCGG 
CCTACCTTCT TGGAGATTAT CGACATGTTA AAAGATGACC TGCATCTTAG TTTTCAGGAA 
GTCTCATTCT ACTACAGTGA CGAGAACAAG CCACCTGAAA CAGATGATTT GGAAATTGAT 
TTTGAAAATA TGGAGAGCAC ACCCCTGGAC CCTTCTTCCT GTTCTTTACG GGATCAGTCG 
AATCGGGGGA ACATTTATGA AGAACACATC CCCTACACCC ATATGAATGG TGGCAGAAAA 
AATGGACGTA TACTGTCTTT GCCACGGTCC AGCCCATCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809042.1
CDS	1517..5458
Translation	MDIRNNISQFSMLEDCTVIEGHLQILLMFTSKPENFRGLRFPKLTMITDYLLLFRVYGLESLKDLFPNLTVIRGTHLFFNYALVIFEMVHLKEIGLYNLMNITRGSVRIEKNNELCYLSTIDWSIILDSVEDNYIELNRDNKEECGDVCPGAGKGKSKCKQIVVNGAFIERCWTQDHCQTVCPSECKGSGCLPDGQCCHSECLGSCRKPNDPTECTACRHYQYQGVCISACPEGSYRFQGWRCIDFNTCQEMHNRCPNKENSCPPYVIHKGECMPDCPSGYIANSTTRTCTPCAGPCPKVCTIYQSVKTIDSVTSAQELRGCTVVNGSLIINIRGGNNIATELEANLGLIEEISGYLKIRRSYALVSLSFFRKLRLIRGEVLEAGNYSFYALDNPSLRQLWDWHKHNLTIIHGKLFFHHNPRLCLAEIHQMEEVTGTKGRQDKNDISTKTNGDQASCEDNLLNFNFIRTSHDMVLLKWDPYWPPDYRDLLGFMVHYKEAPYQNVTEFDGQDACGSNSWTVVDMDAPERSADGKTLSPGCLLRNLKPWTQYAVFVKTLVSGSDEGRTYGAKSKIIYIRTNETIPSVPLDPFSVSNSTSQIILKWKPPSEPNGNVTHYLVYWQEQPEDSELYELDYCNKGLKLPSRTWTPPSDIEENGNINQTDQPSSKCCPCPKTDFQIQKEQDESAFRKTFENYLHNEVFIPRPVRKRRELFGVANGTLPDPVTAPSLFNASSTRAPDETEPKIYSQKVWFKESLMVTGLKHFTGYRIEIHACNHELSMGCSVAAYVSARTMPEATADNVVGPITYEYIEPNIIHLKWQEPKDPNGLIVLYEVHYSRVGGTEEVISCVSQKQYSTDKGAKLRVLTPGNYSVKIRATSLAGNGSWTEPAYFQVPDHPHSSIVKIVTGPVIAVILLLFVLVYCVIQKKKDAEGPVGPLYASSNPEYLSASEVYIPDEWEVPRDKISLLRELGQGSFGMVYEGIAKDIIKGEPEVRVAVKTVNESASLRERIEFLNEASVMKAFSCHHVVRLLGVVSKGQPTLVIMELMTHGDLKSYLRSLRPDAENNPGRPAPTLKEMIQMAAEISDGMAYLNAKKFVHRDLAARNCMVAEDYTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMSPESLKDGVFTTFSDVWSFGVVLWEITSLAEQPYQGLSNEQVLKFVMDGGSLDQPENCPPRLHSLMQMCWQYNPKMRPTFLEIIDMLKDDLHLSFQEVSFYYSDENKPPETDDLEIDFENMESTPLDPSSCSLRDQSNRGNIYEEHIPYTHMNGGRKNGRILSLPRSSPS

Target ORF information:

RefSeq Version	XM_012953588.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis insulin receptor (insr), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953588.3

ORF Insert Sequence:

ATGGATATCC GGAATAACAT TAGTCAGTTC AGTATGCTTG AGGACTGTAC AGTAATTGAA 
GGTCACCTTC AGATCTTGCT TATGTTCACC AGCAAACCAG AGAACTTCCG AGGGTTGAGA 
TTTCCCAAGC TCACCATGAT CACAGATTAC CTCCTGCTTT TCCGGGTCTA TGGCTTAGAA 
AGTCTAAAGG ACCTTTTTCC CAACCTCACA GTTATCCGTG GTACTCACCT CTTTTTCAAC 
TATGCCCTAG TCATCTTTGA GATGGTCCAC CTGAAGGAGA TTGGGCTCTA CAACCTAATG 
AACATTACTA GGGGGTCTGT TAGAATTGAG AAAAATAATG AACTTTGTTA TCTTTCTACG 
ATTGATTGGT CCATAATTCT TGACTCTGTG GAGGATAACT ACATTGAACT CAACAGGGAC 
AATAAAGAGG AGTGTGGAGA TGTTTGTCCT GGTGCAGGAA AAGGCAAGAG CAAATGCAAA 
CAGATAGTAG TCAATGGAGC ATTCATTGAG CGCTGCTGGA CACAGGACCA CTGTCAAACA 
GTGTGCCCTT CAGAATGCAA GGGAAGTGGT TGTTTGCCAG ATGGACAGTG CTGCCACTCA 
GAGTGCCTTG GAAGCTGCAG AAAGCCTAAT GATCCAACTG AGTGTACCGC CTGCCGGCAT 
TACCAGTATC AGGGTGTGTG TATCAGTGCA TGTCCCGAGG GGTCTTACCG GTTCCAGGGT 
TGGCGGTGCA TTGACTTCAA TACCTGTCAA GAAATGCACA ACCGGTGCCC AAATAAGGAA 
AATTCCTGTC CTCCTTATGT CATCCACAAG GGAGAGTGCA TGCCAGATTG CCCATCAGGC 
TATATTGCCA ACTCAACTAC CCGTACTTGT ACACCATGCG CAGGACCTTG CCCCAAAGTT 
TGCACCATCT ACCAAAGTGT CAAGACCATA GACTCTGTGA CATCTGCTCA AGAATTAAGA 
GGGTGCACAG TGGTCAACGG TAGTCTTATC ATCAACATCC GTGGAGGCAA TAATATTGCA 
ACAGAATTAG AAGCTAACCT GGGCCTCATT GAGGAGATTT CTGGATATCT TAAGATCAGA 
AGATCATATG CATTGGTGTC TTTGTCATTC TTCCGCAAGC TGCGTTTAAT TAGGGGAGAA 
GTGTTGGAAG CAGGTAACTA TTCCTTCTAT GCTCTGGATA ATCCCAGTCT ACGCCAGCTG 
TGGGACTGGC ATAAGCACAA CCTGACCATC ATACATGGCA AGCTCTTCTT CCATCACAAC 
CCACGACTAT GTCTAGCGGA GATTCACCAA ATGGAGGAGG TTACAGGAAC AAAGGGCCGG 
CAAGACAAGA ATGATATATC TACAAAGACC AATGGAGATC AGGCATCTTG TGAGGACAAT 
TTACTGAACT TTAACTTTAT AAGGACATCG CATGATATGG TGCTGTTAAA GTGGGATCCT 
TACTGGCCCC CTGATTACAG AGACCTCTTG GGCTTCATGG TGCATTATAA GGAAGCACCC 
TACCAGAATG TCACAGAATT TGATGGTCAG GATGCTTGTG GTTCCAATAG TTGGACAGTG 
GTAGATATGG ATGCCCCAGA ACGAAGTGCT GATGGCAAGA CTCTATCTCC GGGATGTTTG 
CTCCGTAACC TGAAACCATG GACTCAGTAT GCAGTGTTTG TTAAAACACT GGTATCCGGA 
TCAGATGAAG GAAGAACTTA TGGTGCAAAA AGCAAAATTA TCTATATTCG TACCAATGAA 
ACCATCCCCT CTGTTCCTCT GGATCCTTTC TCTGTGTCCA ACTCAACCTC TCAGATTATT 
CTAAAATGGA AACCACCTTC TGAGCCCAAT GGAAATGTCA CTCACTACCT TGTGTACTGG 
CAAGAACAAC CTGAAGACAG TGAACTTTAT GAGCTGGATT ACTGCAATAA AGGTCTTAAG 
TTGCCTTCCA GAACTTGGAC TCCTCCCTCG GATATTGAGG AAAATGGAAA TATAAATCAG 
ACAGACCAAC CTTCATCCAA GTGCTGTCCC TGTCCCAAAA CTGATTTCCA AATCCAGAAA 
GAACAGGATG AATCAGCATT CAGAAAAACA TTTGAAAATT ATCTACATAA TGAAGTTTTT 
ATACCTAGAC CTGTTCGAAA ACGGCGTGAA CTATTTGGAG TAGCCAATGG AACTCTGCCA 
GACCCAGTGA CAGCCCCTTC TCTCTTCAAT GCCTCCTCCA CCCGTGCTCC GGATGAAACA 
GAGCCAAAGA TATATTCACA GAAAGTTTGG TTTAAGGAGT CTCTAATGGT CACTGGCTTA 
AAGCATTTTA CTGGTTACCG CATTGAGATT CATGCCTGCA ACCACGAACT CTCCATGGGA 
TGTAGCGTGG CAGCATATGT TAGTGCAAGG ACAATGCCAG AGGCCACTGC CGACAATGTT 
GTGGGACCCA TCACCTATGA GTATATTGAA CCTAATATAA TCCATTTAAA ATGGCAAGAA 
CCAAAAGATC CAAATGGGCT AATAGTTTTG TATGAGGTGC ATTACTCCCG AGTTGGGGGC 
ACAGAGGAAG TGATTAGCTG CGTGTCACAG AAGCAGTATA GCACAGACAA AGGGGCAAAA 
CTCAGGGTAT TAACCCCAGG GAATTACAGT GTAAAGATCC GTGCAACCTC CCTGGCAGGC 
AATGGATCAT GGACAGAACC AGCCTACTTC CAAGTGCCAG ACCACCCTCA CAGCAGCATA 
GTGAAAATCG TCACTGGTCC AGTAATTGCA GTCATTCTTC TGCTTTTTGT CCTGGTTTAC 
TGTGTTATTC AGAAGAAAAA AGATGCAGAA GGCCCAGTTG GACCACTCTA CGCCTCCTCT 
AATCCTGAAT ATCTTAGTGC AAGTGAAGTC TACATCCCTG ACGAATGGGA GGTACCACGT 
GATAAAATCA GCTTACTGCG AGAATTGGGC CAGGGCTCAT TTGGAATGGT GTACGAGGGT 
ATTGCCAAAG ATATTATCAA GGGAGAGCCA GAAGTTCGTG TTGCTGTGAA AACTGTGAAT 
GAGTCTGCAA GCCTTCGAGA GAGGATAGAA TTCCTAAATG AGGCTTCTGT CATGAAGGCA 
TTTAGCTGCC ACCATGTGGT CCGTCTGCTT GGTGTGGTGT CCAAAGGTCA GCCAACACTT 
GTCATCATGG AGTTAATGAC GCATGGGGAT CTCAAAAGTT ACCTGCGCTC ACTGCGACCT 
GATGCAGAGA ATAACCCAGG ACGTCCTGCT CCTACCCTTA AAGAAATGAT CCAGATGGCA 
GCGGAAATTT CAGATGGCAT GGCATATCTC AATGCTAAAA AGTTTGTACA CAGGGATCTG 
GCTGCTAGGA ACTGCATGGT TGCTGAAGAT TATACTGTGA AAATTGGAGA TTTTGGAATG 
ACACGAGATA TCTATGAGAC AGATTATTAC CGAAAAGGGG GAAAGGGGCT GCTGCCTGTT 
AGATGGATGT CCCCTGAATC ACTGAAAGAT GGAGTGTTCA CAACTTTCTC AGATGTCTGG 
TCCTTTGGAG TTGTTTTATG GGAAATAACG AGTTTGGCAG AACAGCCATA TCAGGGACTT 
TCTAATGAGC AGGTCCTGAA GTTTGTCATG GATGGAGGTT CACTTGACCA ACCGGAGAAT 
TGCCCTCCAA GGCTGCATAG TCTGATGCAG ATGTGCTGGC AGTATAACCC CAAGATGCGG 
CCTACCTTCT TGGAGATTAT CGACATGTTA AAAGATGACC TGCATCTTAG TTTTCAGGAA 
GTCTCATTCT ACTACAGTGA CGAGAACAAG CCACCTGAAA CAGATGATTT GGAAATTGAT 
TTTGAAAATA TGGAGAGCAC ACCCCTGGAC CCTTCTTCCT GTTCTTTACG GGATCAGTCG 
AATCGGGGGA ACATTTATGA AGAACACATC CCCTACACCC ATATGAATGG TGGCAGAAAA 
AATGGACGTA TACTGTCTTT GCCACGGTCC AGCCCATCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

insr ( XM_012953588.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809042.1 Xenopus tropicalis insulin receptor (insr), transcript variant X2, mRNA.

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CloneID	OXa35450
Clone ID Related Accession (Same CDS sequence)	XM_012953588.2 , XM_012953588.3
Accession Version	XM_012953588.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	insulin receptor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012953588.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATCC GGAATAACAT TAGTCAGTTC AGTATGCTTG AGGACTGTAC AGTAATTGAA 
GGTCACCTTC AGATCTTGCT TATGTTCACC AGCAAACCAG AGAACTTCCG AGGGTTGAGA 
TTTCCCAAGC TCACCATGAT CACAGATTAC CTCCTGCTTT TCCGGGTCTA TGGCTTAGAA 
AGTCTAAAGG ACCTTTTTCC CAACCTCACA GTTATCCGTG GTACTCACCT CTTTTTCAAC 
TATGCCCTAG TCATCTTTGA GATGGTCCAC CTGAAGGAGA TTGGGCTCTA CAACCTAATG 
AACATTACTA GGGGGTCTGT TAGAATTGAG AAAAATAATG AACTTTGTTA TCTTTCTACG 
ATTGATTGGT CCATAATTCT TGACTCTGTG GAGGATAACT ACATTGAACT CAACAGGGAC 
AATAAAGAGG AGTGTGGAGA TGTTTGTCCT GGTGCAGGAA AAGGCAAGAG CAAATGCAAA 
CAGATAGTAG TCAATGGAGC ATTCATTGAG CGCTGCTGGA CACAGGACCA CTGTCAAACA 
GTGTGCCCTT CAGAATGCAA GGGAAGTGGT TGTTTGCCAG ATGGACAGTG CTGCCACTCA 
GAGTGCCTTG GAAGCTGCAG AAAGCCTAAT GATCCAACTG AGTGTACCGC CTGCCGGCAT 
TACCAGTATC AGGGTGTGTG TATCAGTGCA TGTCCCGAGG GGTCTTACCG GTTCCAGGGT 
TGGCGGTGCA TTGACTTCAA TACCTGTCAA GAAATGCACA ACCGGTGCCC AAATAAGGAA 
AATTCCTGTC CTCCTTATGT CATCCACAAG GGAGAGTGCA TGCCAGATTG CCCATCAGGC 
TATATTGCCA ACTCAACTAC CCGTACTTGT ACACCATGCG CAGGACCTTG CCCCAAAGTT 
TGCACCATCT ACCAAAGTGT CAAGACCATA GACTCTGTGA CATCTGCTCA AGAATTAAGA 
GGGTGCACAG TGGTCAACGG TAGTCTTATC ATCAACATCC GTGGAGGCAA TAATATTGCA 
ACAGAATTAG AAGCTAACCT GGGCCTCATT GAGGAGATTT CTGGATATCT TAAGATCAGA 
AGATCATATG CATTGGTGTC TTTGTCATTC TTCCGCAAGC TGCGTTTAAT TAGGGGAGAA 
GTGTTGGAAG CAGGTAACTA TTCCTTCTAT GCTCTGGATA ATCCCAGTCT ACGCCAGCTG 
TGGGACTGGC ATAAGCACAA CCTGACCATC ATACATGGCA AGCTCTTCTT CCATCACAAC 
CCACGACTAT GTCTAGCGGA GATTCACCAA ATGGAGGAGG TTACAGGAAC AAAGGGCCGG 
CAAGACAAGA ATGATATATC TACAAAGACC AATGGAGATC AGGCATCTTG TGAGGACAAT 
TTACTGAACT TTAACTTTAT AAGGACATCG CATGATATGG TGCTGTTAAA GTGGGATCCT 
TACTGGCCCC CTGATTACAG AGACCTCTTG GGCTTCATGG TGCATTATAA GGAAGCACCC 
TACCAGAATG TCACAGAATT TGATGGTCAG GATGCTTGTG GTTCCAATAG TTGGACAGTG 
GTAGATATGG ATGCCCCAGA ACGAAGTGCT GATGGCAAGA CTCTATCTCC GGGATGTTTG 
CTCCGTAACC TGAAACCATG GACTCAGTAT GCAGTGTTTG TTAAAACACT GGTATCCGGA 
TCAGATGAAG GAAGAACTTA TGGTGCAAAA AGCAAAATTA TCTATATTCG TACCAATGAA 
ACCATCCCCT CTGTTCCTCT GGATCCTTTC TCTGTGTCCA ACTCAACCTC TCAGATTATT 
CTAAAATGGA AACCACCTTC TGAGCCCAAT GGAAATGTCA CTCACTACCT TGTGTACTGG 
CAAGAACAAC CTGAAGACAG TGAACTTTAT GAGCTGGATT ACTGCAATAA AGGTCTTAAG 
TTGCCTTCCA GAACTTGGAC TCCTCCCTCG GATATTGAGG AAAATGGAAA TATAAATCAG 
ACAGACCAAC CTTCATCCAA GTGCTGTCCC TGTCCCAAAA CTGATTTCCA AATCCAGAAA 
GAACAGGATG AATCAGCATT CAGAAAAACA TTTGAAAATT ATCTACATAA TGAAGTTTTT 
ATACCTAGAC CTGTTCGAAA ACGGCGTGAA CTATTTGGAG TAGCCAATGG AACTCTGCCA 
GACCCAGTGA CAGCCCCTTC TCTCTTCAAT GCCTCCTCCA CCCGTGCTCC GGATGAAACA 
GAGCCAAAGA TATATTCACA GAAAGTTTGG TTTAAGGAGT CTCTAATGGT CACTGGCTTA 
AAGCATTTTA CTGGTTACCG CATTGAGATT CATGCCTGCA ACCACGAACT CTCCATGGGA 
TGTAGCGTGG CAGCATATGT TAGTGCAAGG ACAATGCCAG AGGCCACTGC CGACAATGTT 
GTGGGACCCA TCACCTATGA GTATATTGAA CCTAATATAA TCCATTTAAA ATGGCAAGAA 
CCAAAAGATC CAAATGGGCT AATAGTTTTG TATGAGGTGC ATTACTCCCG AGTTGGGGGC 
ACAGAGGAAG TGATTAGCTG CGTGTCACAG AAGCAGTATA GCACAGACAA AGGGGCAAAA 
CTCAGGGTAT TAACCCCAGG GAATTACAGT GTAAAGATCC GTGCAACCTC CCTGGCAGGC 
AATGGATCAT GGACAGAACC AGCCTACTTC CAAGTGCCAG ACCACCCTCA CAGCAGCATA 
GTGAAAATCG TCACTGGTCC AGTAATTGCA GTCATTCTTC TGCTTTTTGT CCTGGTTTAC 
TGTGTTATTC AGAAGAAAAA AGATGCAGAA GGCCCAGTTG GACCACTCTA CGCCTCCTCT 
AATCCTGAAT ATCTTAGTGC AAGTGAAGTC TACATCCCTG ACGAATGGGA GGTACCACGT 
GATAAAATCA GCTTACTGCG AGAATTGGGC CAGGGCTCAT TTGGAATGGT GTACGAGGGT 
ATTGCCAAAG ATATTATCAA GGGAGAGCCA GAAGTTCGTG TTGCTGTGAA AACTGTGAAT 
GAGTCTGCAA GCCTTCGAGA GAGGATAGAA TTCCTAAATG AGGCTTCTGT CATGAAGGCA 
TTTAGCTGCC ACCATGTGGT CCGTCTGCTT GGTGTGGTGT CCAAAGGTCA GCCAACACTT 
GTCATCATGG AGTTAATGAC GCATGGGGAT CTCAAAAGTT ACCTGCGCTC ACTGCGACCT 
GATGCAGAGA ATAACCCAGG ACGTCCTGCT CCTACCCTTA AAGAAATGAT CCAGATGGCA 
GCGGAAATTT CAGATGGCAT GGCATATCTC AATGCTAAAA AGTTTGTACA CAGGGATCTG 
GCTGCTAGGA ACTGCATGGT TGCTGAAGAT TATACTGTGA AAATTGGAGA TTTTGGAATG 
ACACGAGATA TCTATGAGAC AGATTATTAC CGAAAAGGGG GAAAGGGGCT GCTGCCTGTT 
AGATGGATGT CCCCTGAATC ACTGAAAGAT GGAGTGTTCA CAACTTTCTC AGATGTCTGG 
TCCTTTGGAG TTGTTTTATG GGAAATAACG AGTTTGGCAG AACAGCCATA TCAGGGACTT 
TCTAATGAGC AGGTCCTGAA GTTTGTCATG GATGGAGGTT CACTTGACCA ACCGGAGAAT 
TGCCCTCCAA GGCTGCATAG TCTGATGCAG ATGTGCTGGC AGTATAACCC CAAGATGCGG 
CCTACCTTCT TGGAGATTAT CGACATGTTA AAAGATGACC TGCATCTTAG TTTTCAGGAA 
GTCTCATTCT ACTACAGTGA CGAGAACAAG CCACCTGAAA CAGATGATTT GGAAATTGAT 
TTTGAAAATA TGGAGAGCAC ACCCCTGGAC CCTTCTTCCT GTTCTTTACG GGATCAGTCG 
AATCGGGGGA ACATTTATGA AGAACACATC CCCTACACCC ATATGAATGG TGGCAGAAAA 
AATGGACGTA TACTGTCTTT GCCACGGTCC AGCCCATCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809042.1
CDS	1805..5746
Translation	MDIRNNISQFSMLEDCTVIEGHLQILLMFTSKPENFRGLRFPKLTMITDYLLLFRVYGLESLKDLFPNLTVIRGTHLFFNYALVIFEMVHLKEIGLYNLMNITRGSVRIEKNNELCYLSTIDWSIILDSVEDNYIELNRDNKEECGDVCPGAGKGKSKCKQIVVNGAFIERCWTQDHCQTVCPSECKGSGCLPDGQCCHSECLGSCRKPNDPTECTACRHYQYQGVCISACPEGSYRFQGWRCIDFNTCQEMHNRCPNKENSCPPYVIHKGECMPDCPSGYIANSTTRTCTPCAGPCPKVCTIYQSVKTIDSVTSAQELRGCTVVNGSLIINIRGGNNIATELEANLGLIEEISGYLKIRRSYALVSLSFFRKLRLIRGEVLEAGNYSFYALDNPSLRQLWDWHKHNLTIIHGKLFFHHNPRLCLAEIHQMEEVTGTKGRQDKNDISTKTNGDQASCEDNLLNFNFIRTSHDMVLLKWDPYWPPDYRDLLGFMVHYKEAPYQNVTEFDGQDACGSNSWTVVDMDAPERSADGKTLSPGCLLRNLKPWTQYAVFVKTLVSGSDEGRTYGAKSKIIYIRTNETIPSVPLDPFSVSNSTSQIILKWKPPSEPNGNVTHYLVYWQEQPEDSELYELDYCNKGLKLPSRTWTPPSDIEENGNINQTDQPSSKCCPCPKTDFQIQKEQDESAFRKTFENYLHNEVFIPRPVRKRRELFGVANGTLPDPVTAPSLFNASSTRAPDETEPKIYSQKVWFKESLMVTGLKHFTGYRIEIHACNHELSMGCSVAAYVSARTMPEATADNVVGPITYEYIEPNIIHLKWQEPKDPNGLIVLYEVHYSRVGGTEEVISCVSQKQYSTDKGAKLRVLTPGNYSVKIRATSLAGNGSWTEPAYFQVPDHPHSSIVKIVTGPVIAVILLLFVLVYCVIQKKKDAEGPVGPLYASSNPEYLSASEVYIPDEWEVPRDKISLLRELGQGSFGMVYEGIAKDIIKGEPEVRVAVKTVNESASLRERIEFLNEASVMKAFSCHHVVRLLGVVSKGQPTLVIMELMTHGDLKSYLRSLRPDAENNPGRPAPTLKEMIQMAAEISDGMAYLNAKKFVHRDLAARNCMVAEDYTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMSPESLKDGVFTTFSDVWSFGVVLWEITSLAEQPYQGLSNEQVLKFVMDGGSLDQPENCPPRLHSLMQMCWQYNPKMRPTFLEIIDMLKDDLHLSFQEVSFYYSDENKPPETDDLEIDFENMESTPLDPSSCSLRDQSNRGNIYEEHIPYTHMNGGRKNGRILSLPRSSPS

Target ORF information:

RefSeq Version	XM_012953588.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis insulin receptor (insr), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953588.2

ORF Insert Sequence:

ATGGATATCC GGAATAACAT TAGTCAGTTC AGTATGCTTG AGGACTGTAC AGTAATTGAA 
GGTCACCTTC AGATCTTGCT TATGTTCACC AGCAAACCAG AGAACTTCCG AGGGTTGAGA 
TTTCCCAAGC TCACCATGAT CACAGATTAC CTCCTGCTTT TCCGGGTCTA TGGCTTAGAA 
AGTCTAAAGG ACCTTTTTCC CAACCTCACA GTTATCCGTG GTACTCACCT CTTTTTCAAC 
TATGCCCTAG TCATCTTTGA GATGGTCCAC CTGAAGGAGA TTGGGCTCTA CAACCTAATG 
AACATTACTA GGGGGTCTGT TAGAATTGAG AAAAATAATG AACTTTGTTA TCTTTCTACG 
ATTGATTGGT CCATAATTCT TGACTCTGTG GAGGATAACT ACATTGAACT CAACAGGGAC 
AATAAAGAGG AGTGTGGAGA TGTTTGTCCT GGTGCAGGAA AAGGCAAGAG CAAATGCAAA 
CAGATAGTAG TCAATGGAGC ATTCATTGAG CGCTGCTGGA CACAGGACCA CTGTCAAACA 
GTGTGCCCTT CAGAATGCAA GGGAAGTGGT TGTTTGCCAG ATGGACAGTG CTGCCACTCA 
GAGTGCCTTG GAAGCTGCAG AAAGCCTAAT GATCCAACTG AGTGTACCGC CTGCCGGCAT 
TACCAGTATC AGGGTGTGTG TATCAGTGCA TGTCCCGAGG GGTCTTACCG GTTCCAGGGT 
TGGCGGTGCA TTGACTTCAA TACCTGTCAA GAAATGCACA ACCGGTGCCC AAATAAGGAA 
AATTCCTGTC CTCCTTATGT CATCCACAAG GGAGAGTGCA TGCCAGATTG CCCATCAGGC 
TATATTGCCA ACTCAACTAC CCGTACTTGT ACACCATGCG CAGGACCTTG CCCCAAAGTT 
TGCACCATCT ACCAAAGTGT CAAGACCATA GACTCTGTGA CATCTGCTCA AGAATTAAGA 
GGGTGCACAG TGGTCAACGG TAGTCTTATC ATCAACATCC GTGGAGGCAA TAATATTGCA 
ACAGAATTAG AAGCTAACCT GGGCCTCATT GAGGAGATTT CTGGATATCT TAAGATCAGA 
AGATCATATG CATTGGTGTC TTTGTCATTC TTCCGCAAGC TGCGTTTAAT TAGGGGAGAA 
GTGTTGGAAG CAGGTAACTA TTCCTTCTAT GCTCTGGATA ATCCCAGTCT ACGCCAGCTG 
TGGGACTGGC ATAAGCACAA CCTGACCATC ATACATGGCA AGCTCTTCTT CCATCACAAC 
CCACGACTAT GTCTAGCGGA GATTCACCAA ATGGAGGAGG TTACAGGAAC AAAGGGCCGG 
CAAGACAAGA ATGATATATC TACAAAGACC AATGGAGATC AGGCATCTTG TGAGGACAAT 
TTACTGAACT TTAACTTTAT AAGGACATCG CATGATATGG TGCTGTTAAA GTGGGATCCT 
TACTGGCCCC CTGATTACAG AGACCTCTTG GGCTTCATGG TGCATTATAA GGAAGCACCC 
TACCAGAATG TCACAGAATT TGATGGTCAG GATGCTTGTG GTTCCAATAG TTGGACAGTG 
GTAGATATGG ATGCCCCAGA ACGAAGTGCT GATGGCAAGA CTCTATCTCC GGGATGTTTG 
CTCCGTAACC TGAAACCATG GACTCAGTAT GCAGTGTTTG TTAAAACACT GGTATCCGGA 
TCAGATGAAG GAAGAACTTA TGGTGCAAAA AGCAAAATTA TCTATATTCG TACCAATGAA 
ACCATCCCCT CTGTTCCTCT GGATCCTTTC TCTGTGTCCA ACTCAACCTC TCAGATTATT 
CTAAAATGGA AACCACCTTC TGAGCCCAAT GGAAATGTCA CTCACTACCT TGTGTACTGG 
CAAGAACAAC CTGAAGACAG TGAACTTTAT GAGCTGGATT ACTGCAATAA AGGTCTTAAG 
TTGCCTTCCA GAACTTGGAC TCCTCCCTCG GATATTGAGG AAAATGGAAA TATAAATCAG 
ACAGACCAAC CTTCATCCAA GTGCTGTCCC TGTCCCAAAA CTGATTTCCA AATCCAGAAA 
GAACAGGATG AATCAGCATT CAGAAAAACA TTTGAAAATT ATCTACATAA TGAAGTTTTT 
ATACCTAGAC CTGTTCGAAA ACGGCGTGAA CTATTTGGAG TAGCCAATGG AACTCTGCCA 
GACCCAGTGA CAGCCCCTTC TCTCTTCAAT GCCTCCTCCA CCCGTGCTCC GGATGAAACA 
GAGCCAAAGA TATATTCACA GAAAGTTTGG TTTAAGGAGT CTCTAATGGT CACTGGCTTA 
AAGCATTTTA CTGGTTACCG CATTGAGATT CATGCCTGCA ACCACGAACT CTCCATGGGA 
TGTAGCGTGG CAGCATATGT TAGTGCAAGG ACAATGCCAG AGGCCACTGC CGACAATGTT 
GTGGGACCCA TCACCTATGA GTATATTGAA CCTAATATAA TCCATTTAAA ATGGCAAGAA 
CCAAAAGATC CAAATGGGCT AATAGTTTTG TATGAGGTGC ATTACTCCCG AGTTGGGGGC 
ACAGAGGAAG TGATTAGCTG CGTGTCACAG AAGCAGTATA GCACAGACAA AGGGGCAAAA 
CTCAGGGTAT TAACCCCAGG GAATTACAGT GTAAAGATCC GTGCAACCTC CCTGGCAGGC 
AATGGATCAT GGACAGAACC AGCCTACTTC CAAGTGCCAG ACCACCCTCA CAGCAGCATA 
GTGAAAATCG TCACTGGTCC AGTAATTGCA GTCATTCTTC TGCTTTTTGT CCTGGTTTAC 
TGTGTTATTC AGAAGAAAAA AGATGCAGAA GGCCCAGTTG GACCACTCTA CGCCTCCTCT 
AATCCTGAAT ATCTTAGTGC AAGTGAAGTC TACATCCCTG ACGAATGGGA GGTACCACGT 
GATAAAATCA GCTTACTGCG AGAATTGGGC CAGGGCTCAT TTGGAATGGT GTACGAGGGT 
ATTGCCAAAG ATATTATCAA GGGAGAGCCA GAAGTTCGTG TTGCTGTGAA AACTGTGAAT 
GAGTCTGCAA GCCTTCGAGA GAGGATAGAA TTCCTAAATG AGGCTTCTGT CATGAAGGCA 
TTTAGCTGCC ACCATGTGGT CCGTCTGCTT GGTGTGGTGT CCAAAGGTCA GCCAACACTT 
GTCATCATGG AGTTAATGAC GCATGGGGAT CTCAAAAGTT ACCTGCGCTC ACTGCGACCT 
GATGCAGAGA ATAACCCAGG ACGTCCTGCT CCTACCCTTA AAGAAATGAT CCAGATGGCA 
GCGGAAATTT CAGATGGCAT GGCATATCTC AATGCTAAAA AGTTTGTACA CAGGGATCTG 
GCTGCTAGGA ACTGCATGGT TGCTGAAGAT TATACTGTGA AAATTGGAGA TTTTGGAATG 
ACACGAGATA TCTATGAGAC AGATTATTAC CGAAAAGGGG GAAAGGGGCT GCTGCCTGTT 
AGATGGATGT CCCCTGAATC ACTGAAAGAT GGAGTGTTCA CAACTTTCTC AGATGTCTGG 
TCCTTTGGAG TTGTTTTATG GGAAATAACG AGTTTGGCAG AACAGCCATA TCAGGGACTT 
TCTAATGAGC AGGTCCTGAA GTTTGTCATG GATGGAGGTT CACTTGACCA ACCGGAGAAT 
TGCCCTCCAA GGCTGCATAG TCTGATGCAG ATGTGCTGGC AGTATAACCC CAAGATGCGG 
CCTACCTTCT TGGAGATTAT CGACATGTTA AAAGATGACC TGCATCTTAG TTTTCAGGAA 
GTCTCATTCT ACTACAGTGA CGAGAACAAG CCACCTGAAA CAGATGATTT GGAAATTGAT 
TTTGAAAATA TGGAGAGCAC ACCCCTGGAC CCTTCTTCCT GTTCTTTACG GGATCAGTCG 
AATCGGGGGA ACATTTATGA AGAACACATC CCCTACACCC ATATGAATGG TGGCAGAAAA 
AATGGACGTA TACTGTCTTT GCCACGGTCC AGCCCATCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

insr ( XM_002934379.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934425.2 Xenopus tropicalis insulin receptor (insr), transcript variant X1, mRNA.

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CloneID	OXa04156
Clone ID Related Accession (Same CDS sequence)	XM_002934379.5 , XM_002934379.4
Accession Version	XM_002934379.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	insulin receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934379.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACAGG GTGTATTGTG GGGTGAGGGG CACCCCAATA ATAACCCCAA GAGCAAGGTG 
GGCTGGAAGT CCATTGCGGG AATATTCATC ATTTTTATGC TCATTCTGTG CGACCAAAAC 
GACGGGAAAA TTTGCCCCAG TATGGATATC CGGAATAACA TTAGTCAGTT CAGTATGCTT 
GAGGACTGTA CAGTAATTGA AGGTCACCTT CAGATCTTGC TTATGTTCAC CAGCAAACCA 
GAGAACTTCC GAGGGTTGAG ATTTCCCAAG CTCACCATGA TCACAGATTA CCTCCTGCTT 
TTCCGGGTCT ATGGCTTAGA AAGTCTAAAG GACCTTTTTC CCAACCTCAC AGTTATCCGT 
GGTACTCACC TCTTTTTCAA CTATGCCCTA GTCATCTTTG AGATGGTCCA CCTGAAGGAG 
ATTGGGCTCT ACAACCTAAT GAACATTACT AGGGGGTCTG TTAGAATTGA GAAAAATAAT 
GAACTTTGTT ATCTTTCTAC GATTGATTGG TCCATAATTC TTGACTCTGT GGAGGATAAC 
TACATTGAAC TCAACAGGGA CAATAAAGAG GAGTGTGGAG ATGTTTGTCC TGGTGCAGGA 
AAAGGCAAGA GCAAATGCAA ACAGATAGTA GTCAATGGAG CATTCATTGA GCGCTGCTGG 
ACACAGGACC ACTGTCAAAC AGTGTGCCCT TCAGAATGCA AGGGAAGTGG TTGTTTGCCA 
GATGGACAGT GCTGCCACTC AGAGTGCCTT GGAAGCTGCA GAAAGCCTAA TGATCCAACT 
GAGTGTACCG CCTGCCGGCA TTACCAGTAT CAGGGTGTGT GTATCAGTGC ATGTCCCGAG 
GGGTCTTACC GGTTCCAGGG TTGGCGGTGC ATTGACTTCA ATACCTGTCA AGAAATGCAC 
AACCGGTGCC CAAATAAGGA AAATTCCTGT CCTCCTTATG TCATCCACAA GGGAGAGTGC 
ATGCCAGATT GCCCATCAGG CTATATTGCC AACTCAACTA CCCGTACTTG TACACCATGC 
GCAGGACCTT GCCCCAAAGT TTGCACCATC TACCAAAGTG TCAAGACCAT AGACTCTGTG 
ACATCTGCTC AAGAATTAAG AGGGTGCACA GTGGTCAACG GTAGTCTTAT CATCAACATC 
CGTGGAGGCA ATAATATTGC AACAGAATTA GAAGCTAACC TGGGCCTCAT TGAGGAGATT 
TCTGGATATC TTAAGATCAG AAGATCATAT GCATTGGTGT CTTTGTCATT CTTCCGCAAG 
CTGCGTTTAA TTAGGGGAGA AGTGTTGGAA GCAGGTAACT ATTCCTTCTA TGCTCTGGAT 
AATCCCAGTC TACGCCAGCT GTGGGACTGG CATAAGCACA ACCTGACCAT CATACATGGC 
AAGCTCTTCT TCCATCACAA CCCACGACTA TGTCTAGCGG AGATTCACCA AATGGAGGAG 
GTTACAGGAA CAAAGGGCCG GCAAGACAAG AATGATATAT CTACAAAGAC CAATGGAGAT 
CAGGCATCTT GTGAGGACAA TTTACTGAAC TTTAACTTTA TAAGGACATC GCATGATATG 
GTGCTGTTAA AGTGGGATCC TTACTGGCCC CCTGATTACA GAGACCTCTT GGGCTTCATG 
GTGCATTATA AGGAAGCACC CTACCAGAAT GTCACAGAAT TTGATGGTCA GGATGCTTGT 
GGTTCCAATA GTTGGACAGT GGTAGATATG GATGCCCCAG AACGAAGTGC TGATGGCAAG 
ACTCTATCTC CGGGATGTTT GCTCCGTAAC CTGAAACCAT GGACTCAGTA TGCAGTGTTT 
GTTAAAACAC TGGTATCCGG ATCAGATGAA GGAAGAACTT ATGGTGCAAA AAGCAAAATT 
ATCTATATTC GTACCAATGA AACCATCCCC TCTGTTCCTC TGGATCCTTT CTCTGTGTCC 
AACTCAACCT CTCAGATTAT TCTAAAATGG AAACCACCTT CTGAGCCCAA TGGAAATGTC 
ACTCACTACC TTGTGTACTG GCAAGAACAA CCTGAAGACA GTGAACTTTA TGAGCTGGAT 
TACTGCAATA AAGGTCTTAA GTTGCCTTCC AGAACTTGGA CTCCTCCCTC GGATATTGAG 
GAAAATGGAA ATATAAATCA GACAGACCAA CCTTCATCCA AGTGCTGTCC CTGTCCCAAA 
ACTGATTTCC AAATCCAGAA AGAACAGGAT GAATCAGCAT TCAGAAAAAC ATTTGAAAAT 
TATCTACATA ATGAAGTTTT TATACCTAGA CCTGTTCGAA AACGGCGTGA ACTATTTGGA 
GTAGCCAATG GAACTCTGCC AGACCCAGTG ACAGCCCCTT CTCTCTTCAA TGCCTCCTCC 
ACCCGTGCTC CGGATGAAAC AGAGCCAAAG ATATATTCAC AGAAAGTTTG GTTTAAGGAG 
TCTCTAATGG TCACTGGCTT AAAGCATTTT ACTGGTTACC GCATTGAGAT TCATGCCTGC 
AACCACGAAC TCTCCATGGG ATGTAGCGTG GCAGCATATG TTAGTGCAAG GACAATGCCA 
GAGGCCACTG CCGACAATGT TGTGGGACCC ATCACCTATG AGTATATTGA ACCTAATATA 
ATCCATTTAA AATGGCAAGA ACCAAAAGAT CCAAATGGGC TAATAGTTTT GTATGAGGTG 
CATTACTCCC GAGTTGGGGG CACAGAGGAA GTGATTAGCT GCGTGTCACA GAAGCAGTAT 
AGCACAGACA AAGGGGCAAA ACTCAGGGTA TTAACCCCAG GGAATTACAG TGTAAAGATC 
CGTGCAACCT CCCTGGCAGG CAATGGATCA TGGACAGAAC CAGCCTACTT CCAAGTGCCA 
GACCACCCTC ACAGCAGCAT AGTGAAAATC GTCACTGGTC CAGTAATTGC AGTCATTCTT 
CTGCTTTTTG TCCTGGTTTA CTGTGTTATT CAGAAGAAAA AAGATGCAGA AGGCCCAGTT 
GGACCACTCT ACGCCTCCTC TAATCCTGAA TATCTTAGTG CAAGTGAAGT CTACATCCCT 
GACGAATGGG AGGTACCACG TGATAAAATC AGCTTACTGC GAGAATTGGG CCAGGGCTCA 
TTTGGAATGG TGTACGAGGG TATTGCCAAA GATATTATCA AGGGAGAGCC AGAAGTTCGT 
GTTGCTGTGA AAACTGTGAA TGAGTCTGCA AGCCTTCGAG AGAGGATAGA ATTCCTAAAT 
GAGGCTTCTG TCATGAAGGC ATTTAGCTGC CACCATGTGG TCCGTCTGCT TGGTGTGGTG 
TCCAAAGGTC AGCCAACACT TGTCATCATG GAGTTAATGA CGCATGGGGA TCTCAAAAGT 
TACCTGCGCT CACTGCGACC TGATGCAGAG AATAACCCAG GACGTCCTGC TCCTACCCTT 
AAAGAAATGA TCCAGATGGC AGCGGAAATT TCAGATGGCA TGGCATATCT CAATGCTAAA 
AAGTTTGTAC ACAGGGATCT GGCTGCTAGG AACTGCATGG TTGCTGAAGA TTATACTGTG 
AAAATTGGAG ATTTTGGAAT GACACGAGAT ATCTATGAGA CAGATTATTA CCGAAAAGGG 
GGAAAGGGGC TGCTGCCTGT TAGATGGATG TCCCCTGAAT CACTGAAAGA TGGAGTGTTC 
ACAACTTTCT CAGATGTCTG GTCCTTTGGA GTTGTTTTAT GGGAAATAAC GAGTTTGGCA 
GAACAGCCAT ATCAGGGACT TTCTAATGAG CAGGTCCTGA AGTTTGTCAT GGATGGAGGT 
TCACTTGACC AACCGGAGAA TTGCCCTCCA AGGCTGCATA GTCTGATGCA GATGTGCTGG 
CAGTATAACC CCAAGATGCG GCCTACCTTC TTGGAGATTA TCGACATGTT AAAAGATGAC 
CTGCATCTTA GTTTTCAGGA AGTCTCATTC TACTACAGTG ACGAGAACAA GCCACCTGAA 
ACAGATGATT TGGAAATTGA TTTTGAAAAT ATGGAGAGCA CACCCCTGGA CCCTTCTTCC 
TGTTCTTTAC GGGATCAGTC GAATCGGGGG AACATTTATG AAGAACACAT CCCCTACACC 
CATATGAATG GTGGCAGAAA AAATGGACGT ATACTGTCTT TGCCACGGTC CAGCCCATCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934425.2
CDS	256..4338
Translation	MGQGVLWGEGHPNNNPKSKVGWKSIAGIFIIFMLILCDQNDGKICPSMDIRNNISQFSMLEDCTVIEGHLQILLMFTSKPENFRGLRFPKLTMITDYLLLFRVYGLESLKDLFPNLTVIRGTHLFFNYALVIFEMVHLKEIGLYNLMNITRGSVRIEKNNELCYLSTIDWSIILDSVEDNYIELNRDNKEECGDVCPGAGKGKSKCKQIVVNGAFIERCWTQDHCQTVCPSECKGSGCLPDGQCCHSECLGSCRKPNDPTECTACRHYQYQGVCISACPEGSYRFQGWRCIDFNTCQEMHNRCPNKENSCPPYVIHKGECMPDCPSGYIANSTTRTCTPCAGPCPKVCTIYQSVKTIDSVTSAQELRGCTVVNGSLIINIRGGNNIATELEANLGLIEEISGYLKIRRSYALVSLSFFRKLRLIRGEVLEAGNYSFYALDNPSLRQLWDWHKHNLTIIHGKLFFHHNPRLCLAEIHQMEEVTGTKGRQDKNDISTKTNGDQASCEDNLLNFNFIRTSHDMVLLKWDPYWPPDYRDLLGFMVHYKEAPYQNVTEFDGQDACGSNSWTVVDMDAPERSADGKTLSPGCLLRNLKPWTQYAVFVKTLVSGSDEGRTYGAKSKIIYIRTNETIPSVPLDPFSVSNSTSQIILKWKPPSEPNGNVTHYLVYWQEQPEDSELYELDYCNKGLKLPSRTWTPPSDIEENGNINQTDQPSSKCCPCPKTDFQIQKEQDESAFRKTFENYLHNEVFIPRPVRKRRELFGVANGTLPDPVTAPSLFNASSTRAPDETEPKIYSQKVWFKESLMVTGLKHFTGYRIEIHACNHELSMGCSVAAYVSARTMPEATADNVVGPITYEYIEPNIIHLKWQEPKDPNGLIVLYEVHYSRVGGTEEVISCVSQKQYSTDKGAKLRVLTPGNYSVKIRATSLAGNGSWTEPAYFQVPDHPHSSIVKIVTGPVIAVILLLFVLVYCVIQKKKDAEGPVGPLYASSNPEYLSASEVYIPDEWEVPRDKISLLRELGQGSFGMVYEGIAKDIIKGEPEVRVAVKTVNESASLRERIEFLNEASVMKAFSCHHVVRLLGVVSKGQPTLVIMELMTHGDLKSYLRSLRPDAENNPGRPAPTLKEMIQMAAEISDGMAYLNAKKFVHRDLAARNCMVAEDYTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMSPESLKDGVFTTFSDVWSFGVVLWEITSLAEQPYQGLSNEQVLKFVMDGGSLDQPENCPPRLHSLMQMCWQYNPKMRPTFLEIIDMLKDDLHLSFQEVSFYYSDENKPPETDDLEIDFENMESTPLDPSSCSLRDQSNRGNIYEEHIPYTHMNGGRKNGRILSLPRSSPS

Target ORF information:

RefSeq Version	XM_002934379.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis insulin receptor (insr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934379.4

ORF Insert Sequence:

ATGGGACAGG GTGTATTGTG GGGTGAGGGG CACCCCAATA ATAACCCCAA GAGCAAGGTG 
GGCTGGAAGT CCATTGCGGG AATATTCATC ATTTTTATGC TCATTCTGTG CGACCAAAAC 
GACGGGAAAA TTTGCCCCAG TATGGATATC CGGAATAACA TTAGTCAGTT CAGTATGCTT 
GAGGACTGTA CAGTAATTGA AGGTCACCTT CAGATCTTGC TTATGTTCAC CAGCAAACCA 
GAGAACTTCC GAGGGTTGAG ATTTCCCAAG CTCACCATGA TCACAGATTA CCTCCTGCTT 
TTCCGGGTCT ATGGCTTAGA AAGTCTAAAG GACCTTTTTC CCAACCTCAC AGTTATCCGT 
GGTACTCACC TCTTTTTCAA CTATGCCCTA GTCATCTTTG AGATGGTCCA CCTGAAGGAG 
ATTGGGCTCT ACAACCTAAT GAACATTACT AGGGGGTCTG TTAGAATTGA GAAAAATAAT 
GAACTTTGTT ATCTTTCTAC GATTGATTGG TCCATAATTC TTGACTCTGT GGAGGATAAC 
TACATTGAAC TCAACAGGGA CAATAAAGAG GAGTGTGGAG ATGTTTGTCC TGGTGCAGGA 
AAAGGCAAGA GCAAATGCAA ACAGATAGTA GTCAATGGAG CATTCATTGA GCGCTGCTGG 
ACACAGGACC ACTGTCAAAC AGTGTGCCCT TCAGAATGCA AGGGAAGTGG TTGTTTGCCA 
GATGGACAGT GCTGCCACTC AGAGTGCCTT GGAAGCTGCA GAAAGCCTAA TGATCCAACT 
GAGTGTACCG CCTGCCGGCA TTACCAGTAT CAGGGTGTGT GTATCAGTGC ATGTCCCGAG 
GGGTCTTACC GGTTCCAGGG TTGGCGGTGC ATTGACTTCA ATACCTGTCA AGAAATGCAC 
AACCGGTGCC CAAATAAGGA AAATTCCTGT CCTCCTTATG TCATCCACAA GGGAGAGTGC 
ATGCCAGATT GCCCATCAGG CTATATTGCC AACTCAACTA CCCGTACTTG TACACCATGC 
GCAGGACCTT GCCCCAAAGT TTGCACCATC TACCAAAGTG TCAAGACCAT AGACTCTGTG 
ACATCTGCTC AAGAATTAAG AGGGTGCACA GTGGTCAACG GTAGTCTTAT CATCAACATC 
CGTGGAGGCA ATAATATTGC AACAGAATTA GAAGCTAACC TGGGCCTCAT TGAGGAGATT 
TCTGGATATC TTAAGATCAG AAGATCATAT GCATTGGTGT CTTTGTCATT CTTCCGCAAG 
CTGCGTTTAA TTAGGGGAGA AGTGTTGGAA GCAGGTAACT ATTCCTTCTA TGCTCTGGAT 
AATCCCAGTC TACGCCAGCT GTGGGACTGG CATAAGCACA ACCTGACCAT CATACATGGC 
AAGCTCTTCT TCCATCACAA CCCACGACTA TGTCTAGCGG AGATTCACCA AATGGAGGAG 
GTTACAGGAA CAAAGGGCCG GCAAGACAAG AATGATATAT CTACAAAGAC CAATGGAGAT 
CAGGCATCTT GTGAGGACAA TTTACTGAAC TTTAACTTTA TAAGGACATC GCATGATATG 
GTGCTGTTAA AGTGGGATCC TTACTGGCCC CCTGATTACA GAGACCTCTT GGGCTTCATG 
GTGCATTATA AGGAAGCACC CTACCAGAAT GTCACAGAAT TTGATGGTCA GGATGCTTGT 
GGTTCCAATA GTTGGACAGT GGTAGATATG GATGCCCCAG AACGAAGTGC TGATGGCAAG 
ACTCTATCTC CGGGATGTTT GCTCCGTAAC CTGAAACCAT GGACTCAGTA TGCAGTGTTT 
GTTAAAACAC TGGTATCCGG ATCAGATGAA GGAAGAACTT ATGGTGCAAA AAGCAAAATT 
ATCTATATTC GTACCAATGA AACCATCCCC TCTGTTCCTC TGGATCCTTT CTCTGTGTCC 
AACTCAACCT CTCAGATTAT TCTAAAATGG AAACCACCTT CTGAGCCCAA TGGAAATGTC 
ACTCACTACC TTGTGTACTG GCAAGAACAA CCTGAAGACA GTGAACTTTA TGAGCTGGAT 
TACTGCAATA AAGGTCTTAA GTTGCCTTCC AGAACTTGGA CTCCTCCCTC GGATATTGAG 
GAAAATGGAA ATATAAATCA GACAGACCAA CCTTCATCCA AGTGCTGTCC CTGTCCCAAA 
ACTGATTTCC AAATCCAGAA AGAACAGGAT GAATCAGCAT TCAGAAAAAC ATTTGAAAAT 
TATCTACATA ATGAAGTTTT TATACCTAGA CCTGTTCGAA AACGGCGTGA ACTATTTGGA 
GTAGCCAATG GAACTCTGCC AGACCCAGTG ACAGCCCCTT CTCTCTTCAA TGCCTCCTCC 
ACCCGTGCTC CGGATGAAAC AGAGCCAAAG ATATATTCAC AGAAAGTTTG GTTTAAGGAG 
TCTCTAATGG TCACTGGCTT AAAGCATTTT ACTGGTTACC GCATTGAGAT TCATGCCTGC 
AACCACGAAC TCTCCATGGG ATGTAGCGTG GCAGCATATG TTAGTGCAAG GACAATGCCA 
GAGGCCACTG CCGACAATGT TGTGGGACCC ATCACCTATG AGTATATTGA ACCTAATATA 
ATCCATTTAA AATGGCAAGA ACCAAAAGAT CCAAATGGGC TAATAGTTTT GTATGAGGTG 
CATTACTCCC GAGTTGGGGG CACAGAGGAA GTGATTAGCT GCGTGTCACA GAAGCAGTAT 
AGCACAGACA AAGGGGCAAA ACTCAGGGTA TTAACCCCAG GGAATTACAG TGTAAAGATC 
CGTGCAACCT CCCTGGCAGG CAATGGATCA TGGACAGAAC CAGCCTACTT CCAAGTGCCA 
GACCACCCTC ACAGCAGCAT AGTGAAAATC GTCACTGGTC CAGTAATTGC AGTCATTCTT 
CTGCTTTTTG TCCTGGTTTA CTGTGTTATT CAGAAGAAAA AAGATGCAGA AGGCCCAGTT 
GGACCACTCT ACGCCTCCTC TAATCCTGAA TATCTTAGTG CAAGTGAAGT CTACATCCCT 
GACGAATGGG AGGTACCACG TGATAAAATC AGCTTACTGC GAGAATTGGG CCAGGGCTCA 
TTTGGAATGG TGTACGAGGG TATTGCCAAA GATATTATCA AGGGAGAGCC AGAAGTTCGT 
GTTGCTGTGA AAACTGTGAA TGAGTCTGCA AGCCTTCGAG AGAGGATAGA ATTCCTAAAT 
GAGGCTTCTG TCATGAAGGC ATTTAGCTGC CACCATGTGG TCCGTCTGCT TGGTGTGGTG 
TCCAAAGGTC AGCCAACACT TGTCATCATG GAGTTAATGA CGCATGGGGA TCTCAAAAGT 
TACCTGCGCT CACTGCGACC TGATGCAGAG AATAACCCAG GACGTCCTGC TCCTACCCTT 
AAAGAAATGA TCCAGATGGC AGCGGAAATT TCAGATGGCA TGGCATATCT CAATGCTAAA 
AAGTTTGTAC ACAGGGATCT GGCTGCTAGG AACTGCATGG TTGCTGAAGA TTATACTGTG 
AAAATTGGAG ATTTTGGAAT GACACGAGAT ATCTATGAGA CAGATTATTA CCGAAAAGGG 
GGAAAGGGGC TGCTGCCTGT TAGATGGATG TCCCCTGAAT CACTGAAAGA TGGAGTGTTC 
ACAACTTTCT CAGATGTCTG GTCCTTTGGA GTTGTTTTAT GGGAAATAAC GAGTTTGGCA 
GAACAGCCAT ATCAGGGACT TTCTAATGAG CAGGTCCTGA AGTTTGTCAT GGATGGAGGT 
TCACTTGACC AACCGGAGAA TTGCCCTCCA AGGCTGCATA GTCTGATGCA GATGTGCTGG 
CAGTATAACC CCAAGATGCG GCCTACCTTC TTGGAGATTA TCGACATGTT AAAAGATGAC 
CTGCATCTTA GTTTTCAGGA AGTCTCATTC TACTACAGTG ACGAGAACAA GCCACCTGAA 
ACAGATGATT TGGAAATTGA TTTTGAAAAT ATGGAGAGCA CACCCCTGGA CCCTTCTTCC 
TGTTCTTTAC GGGATCAGTC GAATCGGGGG AACATTTATG AAGAACACAT CCCCTACACC 
CATATGAATG GTGGCAGAAA AAATGGACGT ATACTGTCTT TGCCACGGTC CAGCCCATCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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insr cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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