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CRISPR/Cas9-mediated targeted mutagenesis in Nicotiana tabacum.

Plant Mol Biol.. 2014-10; 
Gao J, Wang G, Ma S, Xie X, Wu X, Zhang X, Wu Y, Zhao P, Xia Q. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400715, China.
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Genome editing is one of the most powerful tools for revealing gene function and improving crop plants. Recently, RNA-guided genome editing using the type II clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein (Cas) system has been used as a powerful and efficient tool for genome editing in various organisms. Here, we report genome editing in tobacco (Nicotiana tabacum) mediated by the CRISPR/Cas9 system. Two genes, NtPDS and NtPDR6, were used for targeted mutagenesis. First, we examined the transient genome editing activity of this system in tobacco protoplasts, insertion and deletion (indel) mutations were observed with frequencies of 16.2-20.3 % after transfecting guide RNA ... More


CRISPR/Cas9 system; Nicotiana tabacum; Targeted mutagenesis; Genome editing