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Transcription Mediated Amplification (TMA)

A target nucleic acid amplification method that uses RNA transcription (RNA Polymerase) and DNA synthesis (reverse transcriptase) to produce RNA amplicon from a target nucleic acid. TMA can be used to target both RNA and DNA.TMA has several other differences in comparison to PCR and LCR:- TMA is isothermal. A water bath or heat block is used instead of a thermal cycler.- TMA produces RNA amplicon rather than DNA amplicon. Since RNA is more labile in the laboratory environment than DNA, this helps reduce the possibility of carry-over contamination.- TMA produces 100-1000 copies PER cycle in contrast to PCR and LCR that produce only two copies PER cycle. This results in a 10 billion fold increase of copies within about 15-30 minutes.From: http://www.gen-probe.com

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