A method to construct a site-specific mutated gene and to select it away from the wild-type. A strain of E. coli with only weak dUTPase activity has high levels of dUTP and consequently incorporates dUTP into a plasmid DNA in competition with dTTP. This plasmid is used in an in vitro system as a template for DNA synthesis with a primer into which the desired mutation has been incorporated. The duplex, containing the wild-type sequence with Us replacing Ts (U-DNA) complementary to a strand with the usual DNA bases and incorporating the mutation, is introduced into a wild-type bacterium that can remove the inappropriate bases from, and thus inactivate, the U-DNA strand and leave the mutated strand to be replicated.
GenSmart Optimization is a free online tool for performing codon optimization to improve gene expression. GenScript's patented algorithms are integrated into the tool to optimize the computing capability of high-performance sequence generation.
GenSmart™ Design is a free online DNA construct design tool developed by GenScript. GenSmart™ Design has two design modules, the Create Construct module for individual plasmid design and the Create Library module for DNA library design.
This online tool shows commonly used genetic codon frequency table in expression host organisms including Escherichia coli and other common host organisms.
Offer gene synthesis of 100% sequence guarantee, free codon optimization, DNA synthesis in as fast as 4 days.
ORF cDNA Clones and Custom Clones
Largest commercial ORF clones database, 70,000 next-day shipping ORF clones.
Plasmid DNA Preparation
High quality plasmids supported by fully-automated platform, 100% full insert sequence accuracy guaranteed.
Express Cloning & Subcloning
$49 and 2 day turnaround, choose from over 150 IP FREE vectors.
Mutation and Library Services
Mutagenesis Portfolio offers several mutation and library services to meet various customers’ needs.
Bridge the gap between chemically synthesized oligos and bological applications.
If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: [email protected]