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pegRNA, or prime editing guide RNA, is a specialized RNA molecule used in prime editing, a next-generation genome-editing technology. Prime editing offers a more precise and flexible method for introducing specific genetic changes, including base insertions, deletions, and substitutions, without creating double-stranded breaks in the DNA, as is the case with CRISPR-Cas9. The pegRNA guides the prime editor to the target DNA sequence and also encodes the desired edit.
pegRNA consists of two key components:
In prime editing, pegRNA works alongside a prime editor enzyme, which is a fusion of a Cas9 nickase (a mutated form of Cas9 that only cuts one strand of DNA) and a reverse transcriptase. The pegRNA directs the prime editor to the target site, where the reverse transcriptase uses the pegRNA’s template to synthesize the new DNA strand, introducing the intended edit with high precision.
Prime editing, utilizing pegRNA, is a valuable technique for precise genome engineering. It is used in:
The innovative tool of pegRNA is expanding the possibilities for genetic research, therapeutic development, and synthetic biology.
GenSmart Optimization is a free online tool for performing codon optimization to improve gene expression. GenScript's patented algorithms are integrated into the tool to optimize the computing capability of high-performance sequence generation.
GenSmart™ Design is a free online DNA construct design tool developed by GenScript. GenSmart™ Design has two design modules, the Create Construct module for individual plasmid design and the Create Library module for DNA library design.
This online tool shows commonly used genetic codon frequency table in expression host organisms including Escherichia coli and other common host organisms.
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If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: [email protected]