A purification procedure to determine if two different molecules (usually proteins) interact. An antibody specific to the protein of interest is added to a cell lysis. Then the antibody-protein complex is pelleted usually using protein-G sepharose which binds most antibodies. If there are any protein/molecules that bind to the first protein, they will also be pelleted. Identification of proteins in the pellet can be determined by Western blot (if an antibody exist) or by sequencing a purified protein band.
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GenSmart™ Codon Optimization
GenSmart Optimization is a free online tool for performing codon optimization to improve gene expression. GenScript's patented algorithms are integrated into the tool to optimize the computing capability of high-performance sequence generation.
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If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: [email protected]