Maxam-Gilbert method (chemical cleavage method)
A technique for sequence analysis of DNA in which four chemical reactions are applied separately, each to cleave the polynucleotide randomly at one of the four bases. Subsequent polyacrylamide-gel electrophoresis separates the products according to chain length, and reveals the distance from the radiolabelled 3'- or 5'-end to the chemically modified base. (see also Sanger method (dideoxynucleotide sequencing))Maxam, A.M. and Gilbert, W. (1980) Methods Enzymol. 65, 499-560
- Molecular Biology
Related Biology Tools
GenSmart™ Codon Optimization
GenSmart Optimization is a free online tool for performing codon optimization to improve gene expression. GenScript's patented algorithms are integrated into the tool to optimize the computing capability of high-performance sequence generation.
DNA Construct Design Tool
GenSmart™ Design is a free online DNA construct design tool developed by GenScript. GenSmart™ Design has two design modules, the Create Construct module for individual plasmid design and the Create Library module for DNA library design.
Codon Frequency Tables
This online tool shows commonly used genetic codon frequency table in expression host organisms including Escherichia coli and other common host organisms.
Service and Products
Offer gene synthesis of 100% sequence guarantee, free codon optimization, DNA synthesis in as fast as 4 days.
ORF cDNA Clones and Custom Clones
Largest commercial ORF clones database, 70,000 next-day shipping ORF clones.
Plasmid DNA Preparation
High quality plasmids supported by fully-automated platform, 100% full insert sequence accuracy guaranteed.
Express Cloning & Subcloning
and 2 day turnaround, choose from over 150 IP FREE vectors.
Mutation and Library Services
Mutagenesis Portfolio offers several mutation and library services to meet various customers’ needs.
Bridge the gap between chemically synthesized oligos and bological applications.
If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: [email protected]