List by Alphabet: A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
A variant of the Sanger method of DNA sequencing. A primer is extended by a polymerase to generate a population of newly synthesized deoxyribonucleotides of assorted lengths; the unused dNTPs are removed, and polymerization continues in four pairs of plus and minus reaction mixtures; the minus mixtures have three NTPs and the plus mixtures have only one. After a second polymerization, the mixtures are fractionated by gel electrophoresis, and each plus and minus pair is compared to indicate the length of the new polydeoxyribonucleotide (by the mobilities of the bands) and the position at which polymerization had terminated as a result of the absence of the missing dNTP. Wu, R. (1994) Trends Biochem. Sci. 19, 429-433
Check our latest news: Next Generation Sequencing
GenSmart Optimization is a free online tool for performing codon optimization to improve gene expression. GenScript's patented algorithms are integrated into the tool to optimize the computing capability of high-performance sequence generation.
GenSmart™ Design is a free online DNA construct design tool developed by GenScript. GenSmart™ Design has two design modules, the Create Construct module for individual plasmid design and the Create Library module for DNA library design.
This online tool shows commonly used genetic codon frequency table in expression host organisms including Escherichia coli and other common host organisms.
Gene Synthesis
Offer gene synthesis of 100% sequence guarantee, free codon optimization, DNA synthesis in as fast as 4 days.
ORF cDNA Clones and Custom Clones
Largest commercial ORF clones database, 70,000 next-day shipping ORF clones.
Plasmid DNA Preparation
High quality plasmids supported by fully-automated platform, 100% full insert sequence accuracy guaranteed.
Express Cloning & Subcloning
$49 and 2 day turnaround, choose from over 150 IP FREE vectors.
Mutation and Library Services
Mutagenesis Portfolio offers several mutation and library services to meet various customers’ needs.
Oligo Synthesis
Bridge the gap between chemically synthesized oligos and bological applications.
If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: [email protected]
