A technique for characterization or preparation of subcellular particles. A swinging-bucket-type centrifuge tube is filled with a sucrose gradient, the bottom of which is most dense and the top least dense. A suspension of the particles is layered over the top of the solution, and centrifugation separates the particles within the gradient according to their density. At the termination of centrifugation the bottom of the tube is pierced and the sucrose, with particles of equal density, drips into a series of receiving tubes.
- Cell Engineering
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