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A graphical method for determination of the type of enzyme inhibition and the dissociation constant (Ki) for an enzyme-inhibitor complex. The effect on the enzymic rate (v) is determined at two or more substrate concentrations, and over a range of inhibitor concentrations (I). In a plot of 1/v against I, data for each substrate concentration fall on straight lines that intersect at I=-Ki and 1/v=1/Vmax (competitive inhibition), or that intersect on the abscissa (1/v=0) at I=-Ki (non-competitive inhibition). For uncompetitive inhibition, the lines are parallel. (see also Cornish-Bowden plot; Easson and Stedman plot; Hunter and Downs plot)
Learn more about restriction enzymes.
GenSmart Optimization is a free online tool for performing codon optimization to improve gene expression. GenScript's patented algorithms are integrated into the tool to optimize the computing capability of high-performance sequence generation.
GenSmart™ Design is a free online DNA construct design tool developed by GenScript. GenSmart™ Design has two design modules, the Create Construct module for individual plasmid design and the Create Library module for DNA library design.
This online tool shows commonly used genetic codon frequency table in expression host organisms including Escherichia coli and other common host organisms.
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Mutation and Library Services
Mutagenesis Portfolio offers several mutation and library services to meet various customers’ needs.
Oligo Synthesis
Bridge the gap between chemically synthesized oligos and bological applications.
If you know of any terms that have been omitted from this glossary that you feel would be useful to include, please send detail to the Editorial Office at GenScript: [email protected]