Enzymes Start with A

PCR Cloning

GenScript List of Common Restriction Enzyme Sites

** This list is collected to facilitate your research, and currently GenScript does not have restriction enzymes for sale.

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Enzyme	Prototype	Sequence	Cut Site	Overhang
AaaI	XmaIII	CGGCCG	C^GGCCG GCCGG^C	5' - CCGG
AagI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
AarI		CACCTGC	CACCTGCNNNN^NNNN GTGGACGNNNNNNNN^	5' - NNNN
AasI	DrdI	GACNNNNNNGTC	GACNNNN^NNGTC CTGNN^NNNNCAG	NN - 3'
AatI	StuI	AGGCCT	AGG^CCT TCC^GGA	Blunt
AatII		GACGTC	GACGT^C C^TGCAG	ACGT - 3'
AauI	Bsp1407I	TGTACA	T^GTACA ACATG^T	5' - CATG
AbaI	BclI	TGATCA	T^GATCA ACTAG^T	5' - CTAG
AbeI	BbvCI	CCTCAGC	CC^TCAGC GGAGT^CG	5' - AGT
AbrI	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
AccI		GTMKAC	GT^MKAC CAMK^TG	5' - MK
AccII	FnuDII	CGCG	CG^CG GC^GC	Blunt
AccIII	BspMII	TCCGGA	T^CCGGA AGGCC^T	5' - GGCC
Acc16I	MstI	TGCGCA	TGC^GCA ACG^CGT	Blunt
Acc36I	BspMI	ACCTGC	ACCTGCNNNN^NNNN TGGACGNNNNNNNN^	5' - NNNN
Acc65I	KpnI	GGTACC	G^GTACC CCATG^G	5' - CATG
Acc113I	ScaI	AGTACT	AGT^ACT TCA^TGA	Blunt
AccB1I	HgiCI	GGYRCC	G^GYRCC CCYRG^G	5' - CYRG
AccB2I	HaeII	RGCGCY	RGCGC^Y R^CGCGY	GCGC - 3'
AccB7I	PflMI	CCANNNNNTGG	CCANNNN^NTGG GGTN^NNNNACC	NNN - 3'
AccBSI	BsrBI	CCGCTC	CCG^CTC GGC^GAG	Blunt
AccEBI	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
AceI	TseI	GCWGC	G^CWGC CGWC^G	5' - GWC
AceII	NheI	GCTAGC	GCTAG^C C^GATCG	CTAG - 3'
AceIII		CAGCTC	CAGCTCNNNNNNN^NNNN GTCGAGNNNNNNNNNNN^	5' - NNNN
AciI		CCGC	C^CGC GGC^G	5' - GC
AclI		AACGTT	AA^CGTT TTGC^AA	5' - GC
AclNI	SpeI	ACTAGT	A^CTAGT TGATC^A	5' - GATC
AclWI	BinI	GGATC	GGATCNNNN^N CCTAGNNNNN^	5' - N
AcpI	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
AcpII	PflMI	CCANNNNNTGG	CCANNNN^NTGG GGTN^NNNNACC	NNN - 3'
AcrII	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
AcsI	ApoI	RAATTY	R^AATTY RTTAA^Y	5' - TTAA
AcuI	Eco57I	CTGAAG	CTGAAGNNNNNNNNNNNNNNNN^ GACTTCNNNNNNNNNNNNNN^NN	NN - 3'
AcvI	PmaCI	CACGTG	CAC^GTG GTG^CAC	Blunt
AcyI		GRCGYC	GR^CGYC CRGC^YG	5' - GC
AdeI	DraIII	CACNNNGTG	CACNNN^GTG GTG^NNNCAC	NNN - 3'
AeuI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
AfaI	RsaI	GTAC	GT^AC CA^TG	Blunt
Afa22MI	PvuI	CGATCG	CGAT^CG GC^TAGC	AT - 3'
Afa16RI	PvuI	CGATCG	CGAT^CG GC^TAGC	AT - 3'
AfeI	Eco47III	AGCGCT	AGC^GCT TCG^CGA	Blunt
AflI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
AflII		CTTAAG	C^TTAAG GAATT^C	5' - AATT
AflIII		ACRYGT	A^CRYGT TGRYC^A	5' - GRYC
AgeI		ACCGGT	A^CCGGT TGGCC^A	5' - GGCC
AglI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
AhaI	CauII	CCSGG	CC^SGG GGS^CC	5' - S
AhaII	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
AhaIII		TTTAAA	TTT^AAA AAA^TTT	Blunt
AhaB8I	KpnI	GGTACC	G^GTACC CCATG^G	5' - CATG
AhdI	Eam1105I	GACNNNNNGTC	GACNNN^NNGTC CTGNN^NNNCAG	N - 3'
AhlI	SpeI	ACTAGT	A^CTAGT TGATC^A	5' - GATC
AhyI	SmaI	CCCGGG	C^CCGGG GGGCC^C	5' - GGCC
AitI	Eco47III	AGCGCT	AGC^GCT TCG^CGA	Blunt
AjnI	EcoRII	CCWGG	^CCWGG GGWCC^	5' - GGWCC
AjoI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
AleI	OliI	CACNNNNGTG	CACNN^NNGTG GTGNN^NNCAC	Blunt
AlfI		GCANNNNNNTGC	GCANNNNNNTGCNNNNNNNNNNNN^ CGTNNNNNNACGNNNNNNNNNN^NN	NN - 3'
AliI	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
AliAJI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
AloI		GAACNNNNNNTCC	GAACNNNNNNTCCNNNNNNNNNNNN^ CTTGNNNNNNAGGNNNNNNN^NNNNN	NNNNN - 3'
AluI		AGCT	AG^CT TC^GA	Blunt
AlwI	BinI	GGATC	GGATCNNNN^N CCTAGNNNNN^	5' - N
Alw21I	HgiAI	GWGCWC	GWGCW^C C^WCGWG	WGCW - 3'
Alw26I	BsmAI	GTCTC	GTCTCN^NNNN CAGAGNNNNN^	5' - NNNN
Alw44I	ApaLI	GTGCAC	G^TGCAC CACGT^G	5' - ACGT
AlwNI		CAGNNNCTG	CAGNNN^CTG GTC^NNNGAC	NNN - 3'
AlwXI	BbvI	GCAGC	GCAGCNNNNNNNN^NNNN CGTCGNNNNNNNNNNNN^	5' - NNNN
Ama87I	AvaI	CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
AocI	SauI	CCTNAGG	CC^TNAGG GGANT^CC	5' - ANT
AocII	SduI	GDGCHC	GDGCH^C C^DCGHG	DGCH - 3'
AorI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
Aor13HI	BspMII	TCCGGA	T^CCGGA AGGCC^T	5' - GGCC
Aor51HI	Eco47III	AGCGCT	AGC^GCT TCG^CGA	Blunt
AosI	MstI	TGCGCA	TGC^GCA ACG^CGT	Blunt
AosII	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
ApaI		GGGCCC	GGGCC^C C^CCGGG	GGCC - 3'
ApaBI		GCANNNNNTGC	GCANNNNN^TGC CGT^NNNNNACG	NNNNN - 3'
ApaCI	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
ApaLI		GTGCAC	G^TGCAC CACGT^G	5' - ACGT
ApaORI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
ApeKI	TseI	GCWGC	G^CWGC CGWC^G	5' - GWC
ApiI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
ApoI		RAATTY	R^AATTY RTTAA^Y	5' - TTAA
ApyI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
AquI	AvaI	CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
AscI		GGCGCGCC	GG^CGCGCC CCGCGC^GG	5' - GCGC
AseI	VspI	ATTAAT	AT^TAAT TAAT^TA	5' - AT
AseII	CauII	CCSGG	CC^SGG GGS^CC	5' - S
AsiI	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
AsiAI	AgeI	ACCGGT	A^CCGGT TGGCC^A	5' - GGCC
AsiSI	SgfI	GCGATCGC	GCGAT^CGC CGC^TAGCG	AT - 3'
AsnI	VspI	ATTAAT	AT^TAAT TAAT^TA	5' - AT
AspI	Tth111I	GACNNNGTC	GACN^NNGTC CTGNN^NCAG	5' - N
Asp700I	XmnI	GAANNNNTTC	GAANN^NNTTC CTTNN^NNAAG	Blunt
Asp713I	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
Asp718I	KpnI	GGTACC	G^GTACC CCATG^G	5' - CATG
Asp745I	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
AspAI	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
AspA2I	AvrII	CCTAGG	C^CTAGG GGATC^C	5' - GATC
AspEI	Eam1105I	GACNNNNNGTC	GACNNN^NNGTC CTGNN^NNNCAG	N - 3'
AspHI	HgiAI	GWGCWC	GWGCW^C C^WCGWG	WGCW - 3'
Asp10HI	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
Asp10HII	PflMI	CCANNNNNTGG	CCANNNN^NTGG GGTN^NNNNACC	NNN - 3'
Asp26HI	BsmI	GAATGC	GAATGCN^ CTTAC^GN	CN - 3'
Asp27HI	BsmI	GAATGC	GAATGCN^ CTTAC^GN	CN - 3'
Asp35HI	BsmI	GAATGC	GAATGCN^ CTTAC^GN	CN - 3'
Asp36HI	BsmI	GAATGC	GAATGCN^ CTTAC^GN	CN - 3'
Asp40HI	BsmI	GAATGC	GAATGCN^ CTTAC^GN	CN - 3'
Asp50HI	BsmI	GAATGC	GAATGCN^ CTTAC^GN	CN - 3'
AspLEI	HhaI	GCGC	GCG^C C^GCG	CG - 3'
AspMI	StuI	AGGCCT	AGG^CCT TCC^GGA	Blunt
AspMDI	MboI	GATC	^GATC CTAG^	5' - CTAG
AspNI	NlaIV	GGNNCC	GGN^NCC CCN^NGG	Blunt
AspS9I	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
AssI	ScaI	AGTACT	AGT^ACT TCA^TGA	Blunt
AstWI	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
AsuI		GGNCC	G^GNCC CCNG^G	5' - CNG
AsuII		TTCGAA	TT^CGAA AAGC^TT	5' - GC
AsuIII	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
AsuC2I	CauII	CCSGG	CC^SGG GGS^CC	5' - S
AsuHPI	HphI	GGTGA	GGTGANNNNNNNN^ CCACTNNNNNNN^N	N - 3'
AsuNHI	NheI	GCTAGC	G^CTAGC CGATC^G	5' - GATC
AtsI	Tth111I	GACNNNGTC	GACN^NNGTC CTGNN^NCAG	5' - N
AvaI		CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
AvaII		GGWCC	G^GWCC CCWG^G	5' - CWG
AvcI	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
AviII	MstI	TGCGCA	TGC^GCA ACG^CGT	Blunt
AvrII		CCTAGG	C^CTAGG GGATC^C	5' - GATC
AvrBII	AvrII	CCTAGG	C^CTAGG GGATC^C	5' - GATC
AxyI	SauI	CCTNAGG	CC^TNAGG GGANT^CC	5' - ANT
AanI	PsiI	TTATAA	TTA^TAA AAT^ATT	Blunt
AbsI		CCTCGAGG	CC^TCGAGG GGAGCT^CC	5' - AGCT
AcoI	CfrI	YGGCCR	Y^GGCCR YCCGG^R	5' - CCGG
AfiI		GCNNNNNNNGG	GCNNNNN^NNGG CGNN^NNNNNCC	NNN - 3'
AjiI	BtrI	CACGTC	CAC^GTC GTG^CAG	Blunt
AjuI			^ ^	Blunt
AluBI	AluI	AGCT	AG^CT TC^GA	Blunt
AsiGI	AgeI	ACCGGT	A^CCGGT TGGCC^A	5' - GGCC