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PCR Cloning

GenScript List of Common Restriction Enzyme Sites

** This list is collected to facilitate your research, and currently GenScript does not have restriction enzymes for sale.

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Enzyme Prototype Sequence Cut Site Overhang
DdeI CTNAG C^TNAG
GANT^C		 5' - ANT
DmaI PvuII CAGCTG CAG^CTG
GTC^GAC		 Blunt
DpaI ScaI AGTACT AGT^ACT
TCA^TGA		 Blunt
DpnI GATC GA^TC
CT^AG		 Blunt
DpnII MboI GATC ^GATC
CTAG^		 5' - CTAG
DraI AhaIII TTTAAA TTT^AAA
AAA^TTT		 Blunt
DraII RGGNCCY RG^GNCCY
RCCNG^GY		 5' - CNG
DraIII CACNNNGTG CACNNN^GTG
GTG^NNNCAC		 NNN - 3'
DrdI GACNNNNNNGTC GACNNNN^NNGTC
CTGNN^NNNNCAG		 NN - 3'
DriI Eam1105I GACNNNNNGTC GACNNN^NNGTC
CTGNN^NNNCAG		 N - 3'
DsaI CCRYGG C^CRYGG
GGRYC^C		 5' - GRYC
DsaII HaeIII GGCC GG^CC
CC^GG		 Blunt
DsaIII XhoII RGATCY R^GATCY
RCTAG^Y		 5' - CTAG
DsaIV AvaII GGWCC G^GWCC
CCWG^G		 5' - CWG
DsaV ScrFI CCNGG ^CCNGG
GGNCC^		 5' - GGNCC
DseDI DrdI GACNNNNNNGTC GACNNNN^NNGTC
CTGNN^NNNNCAG		 NN - 3'
DinI GGCGCC GGC^GCC
CCG^CGG		 Blunt