Enzymes Start with S

PCR Cloning

GenScript List of Common Restriction Enzyme Sites

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Enzyme	Prototype	Sequence	Cut Site	Overhang
SacI		GAGCTC	GAGCT^C C^TCGAG	AGCT - 3'
SacII		CCGCGG	CCGC^GG GG^CGCC	GC - 3'
SacNI	HgiJII	GRGCYC	GRGCY^C C^RCGYG	RGCY - 3'
SalI		GTCGAC	G^TCGAC CAGCT^G	5' - AGCT
SalPI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
SanDI		GGGWCCC	GG^GWCCC CCCWG^GG	5' - CWG
SapI		GCTCTTC	GCTCTTCN^NNN CGAGAAGNNNN^	5' - NNN
SarI	StuI	AGGCCT	AGG^CCT TCC^GGA	Blunt
SatI	Fnu4HI	GCNGC	GC^NGC CGN^CG	5' - N
SauI		CCTNAGG	CC^TNAGG GGANT^CC	5' - ANT
Sau96I	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
Sau3239I	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
Sau3AI	MboI	GATC	^GATC CTAG^	5' - CTAG
SauBMKI	NaeI	GCCGGC	GCC^GGC CGG^CCG	Blunt
SauHPI	NaeI	GCCGGC	GCC^GGC CGG^CCG	Blunt
SauLPI	NaeI	GCCGGC	GCC^GGC CGG^CCG	Blunt
SauLPII	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
SauMI	MboI	GATC	^GATC CTAG^	5' - CTAG
SauNI	NaeI	GCCGGC	GCC^GGC CGG^CCG	Blunt
SauSI	NaeI	GCCGGC	GCC^GGC CGG^CCG	Blunt
SbfI	Sse8387I	CCTGCAGG	CCTGCA^GG GG^ACGTCC	TGCA - 3'
Sbi68I	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
Sbo13I	NruI	TCGCGA	TCG^CGA AGC^GCT	Blunt
SbvI	HaeIII	GGCC	GG^CC CC^GG	Blunt
ScaI		AGTACT	AGT^ACT TCA^TGA	Blunt
SceIII	NaeI	GCCGGC	G^CCGGC CGGCC^G	5' - GGCC
SchI	PleI	GAGTC	GAGTCNNNNN^ CTCAGNNNNN^	Blunt
SchZI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
SciI	XhoI	CTCGAG	CTC^GAG GAG^CTC	Blunt
SciNI	HhaI	GCGC	G^CGC CGC^G	5' - GC
ScrFI		CCNGG	CC^NGG GGN^CC	5' - N
SdaI	Sse8387I	CCTGCAGG	CCTGCA^GG GG^ACGTCC	TGCA - 3'
SdiI	SfiI	GGCCNNNNNGGCC	GGCCNNNN^NGGCC CCGGN^NNNNCCGG	NNN - 3'
SduI		GDGCHC	GDGCH^C C^DCGHG	DGCH - 3'
SecI		CCNNGG	C^CNNGG GGNNC^C	5' - GNNC
SelI	FnuDII	CGCG	^CGCG GCGC^	5' - GCGC
SenPT16I	XmaIII	CGGCCG	C^GGCCG GCCGG^C	5' - CCGG
SenPT14bI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
SepI	AvaIII	ATGCAT	ATGCA^T T^ACGTA	TGCA - 3'
SexAI		ACCWGGT	A^CCWGGT TGGWCC^A	5' - GGWCC
SexBI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
SexCI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
SfaI	HaeIII	GGCC	GG^CC CC^GG	Blunt
SfaNI		GCATC	GCATCNNNNN^NNNN CGTAGNNNNNNNNN^	5' - NNNN
SfcI	SfeI	CTRYAG	C^TRYAG GARYT^C	5' - ARYT
SfeI		CTRYAG	C^TRYAG GARYT^C	5' - ARYT
SfiI		GGCCNNNNNGGCC	GGCCNNNN^NGGCC CCGGN^NNNNCCGG	NNN - 3'
SflI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
SfoI	NarI	GGCGCC	GGC^GCC CCG^CGG	Blunt
Sfr274I	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
Sfr303I	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
SfuI	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
SgfI		GCGATCGC	GCGAT^CGC CGC^TAGCG	AT - 3'
SgrAI		CRCCGGYG	CR^CCGGYG GRGGCC^YC	5' - GGCC
SgrBI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
SimI		GGGTC	GG^GTC CCCAG^	5' - CAG
SinI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
SlaI	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
SleI	EcoRII	CCWGG	^CCWGG GGWCC^	5' - GGWCC
Slu1777I	NaeI	GCCGGC	GCC^GGC CGG^CCG	Blunt
SmaI		CCCGGG	CCC^GGG GGG^CCC	Blunt
SmiI	SwaI	ATTTAAAT	ATTT^AAAT TAAA^TTTA	Blunt
SmiMI	MslI	CAYNNNNRTG	CAYNN^NNRTG GTYNN^NNRAC	Blunt
SmlI		CTYRAG	C^TYRAG GAYRT^C	5' - AYRT
SmuI	FauI	CCCGC	CCCGCNNNN^NN GGGCGNNNNNN^	5' - NN
SmuEI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
SnaBI		TACGTA	TAC^GTA ATG^CAT	Blunt
SniI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
SnoI	ApaLI	GTGCAC	G^TGCAC CACGT^G	5' - ACGT
SolI	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
Sol10179I	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
SpaHI	SphI	GCATGC	GCATG^C C^GTACG	CATG - 3'
SpeI		ACTAGT	A^CTAGT TGATC^A	5' - GATC
SphI		GCATGC	GCATG^C C^GTACG	CATG - 3'
SplI		CGTACG	C^GTACG GCATG^C	5' - CATG
SpmI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
SpoI	NruI	TCGCGA	TCG^CGA AGC^GCT	Blunt
SpuI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
SrfI		GCCCGGGC	GCCC^GGGC CGGG^CCCG	Blunt
SrlI	NaeI	GCCGGC	G^CCGGC CGGCC^G	5' - GGCC
Srl5DI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
Srl32DII	EcoRI	GAATTC	G^AATTC CTTAA^G	5' - TTAA
Srl55DI	EcoRI	GAATTC	G^AATTC CTTAA^G	5' - TTAA
Srl56DI	SfeI	CTRYAG	C^TRYAG GARYT^C	5' - ARYT
SruI	AhaIII	TTTAAA	TTT^AAA AAA^TTT	Blunt
Sru4DI	VspI	ATTAAT	AT^TAAT TAAT^TA	5' - AT
Sru30DI	StuI	AGGCCT	AGG^CCT TCC^GGA	Blunt
SsbI	HindIII	AAGCTT	A^AGCTT TTCGA^A	5' - TCGA
SscL1I	HinfI	GANTC	G^ANTC CTNA^G	5' - TNA
Sse9I	TspEI	AATT	^AATT TTAA^	5' - TTAA
Sse232I		CGCCGGCG	CG^CCGGCG GCGGCC^GC	5' - GGCC
Sse1825I	SanDI	GGGWCCC	GG^GWCCC CCCWG^GG	5' - CWG
Sse8387I		CCTGCAGG	CCTGCA^GG GG^ACGTCC	TGCA - 3'
Sse8647I		AGGWCCT	AG^GWCCT TCCWG^GA	5' - CWG
SseAI	NarI	GGCGCC	GG^CGCC CCGC^GG	5' - GC
SseBI	StuI	AGGCCT	AGG^CCT TCC^GGA	Blunt
SshAI	SauI	CCTNAGG	CC^TNAGG GGANT^CC	5' - ANT
SsiI	AciI	CCGC	C^CGC GGC^G	5' - GC
SsiAI	MboI	GATC	^GATC CTAG^	5' - CTAG
SsiBI	MboI	GATC	^GATC CTAG^	5' - CTAG
SslI	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
SsoI	EcoRI	GAATTC	G^AATTC CTTAA^G	5' - TTAA
SsoII	ScrFI	CCNGG	^CCNGG GGNCC^	5' - GGNCC
SspI		AATATT	AAT^ATT TTA^TAA	Blunt
Ssp1I	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
Ssp4800I	Bsp1407I	TGTACA	T^GTACA ACATG^T	5' - CATG
Ssp5230I	AatII	GACGTC	GACGT^C C^TGCAG	ACGT - 3'
Ssp27144I	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
SspAI	EcoRII	CCWGG	^CCWGG GGWCC^	5' - GGWCC
SspBI	Bsp1407I	TGTACA	T^GTACA ACATG^T	5' - CATG
SspCI	NaeI	GCCGGC	GCC^GGC CGG^CCG	Blunt
SspD5I	HphI	GGTGA	GGTGANNNNNNNN^ CCACTNNNNNNNN^	Blunt
SspD5II	AvaIII	ATGCAT	ATGCA^T T^ACGTA	TGCA - 3'
SspRFI	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
SsrI	HpaI	GTTAAC	GTT^AAC CAA^TTG	Blunt
SstI	SacI	GAGCTC	GAGCT^C C^TCGAG	AGCT - 3'
SstII	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
Sst12I	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
SteI	StuI	AGGCCT	AGG^CCT TCC^GGA	Blunt
SthI	KpnI	GGTACC	G^GTACC CCATG^G	5' - CATG
Sth117I	EcoRII	CCWGG	CC^WGG GGW^CC	5' - W
Sth132I		CCCG	CCCGNNNN^NNNN GGGCNNNNNNNN^	5' - NNNN
Sth134I	HpaII	CCGG	C^CGG GGC^C	5' - GC
Sth368I	MboI	GATC	^GATC CTAG^	5' - CTAG
StrI	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
StsI	FokI	GGATG	GGATGNNNNNNNNNN^NNNN CCTACNNNNNNNNNNNNNN^	5' - NNNN
StuI		AGGCCT	AGG^CCT TCC^GGA	Blunt
StyI		CCWWGG	C^CWWGG GGWWC^C	5' - GWWC
StyD4I	ScrFI	CCNGG	^CCNGG GGNCC^	5' - GGNCC
SuaI	HaeIII	GGCC	GG^CC CC^GG	Blunt
SunI	SplI	CGTACG	C^GTACG GCATG^C	5' - CATG
SurI	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
SviI	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
SwaI		ATTTAAAT	ATTT^AAAT TAAA^TTTA	Blunt
SetI		ASST	ASST^ ^TSSA	ASST - 3'
SfaAI		GCGATCGC	GCGAT^CGC CGC^TAGCG	AT - 3'
SgrDI		CGTCGACG	CG^TCGACG GCAGCT^GC	5' - AGCT
SgsI		GGCGCGCC	GG^CGCGCC CCGCGC^GG	5' - GCGC
SmoI		CTYRAG	C^TYRAG GAYRT^C	5' - AYRT
SspDI		GGCGCC	G^GCGCC CCGCG^G	5' - CGCG