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PCR Cloning

GenScript List of Common Restriction Enzyme Sites

** This list is collected to facilitate your research, and currently GenScript does not have restriction enzymes for sale.

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Enzyme Prototype Sequence Cut Site Overhang
KasI NarI GGCGCC G^GCGCC
CCGCG^G		 5' - CGCG
Kaz48kI DraII RGGNCCY RGGNC^CY
RC^CNGGY		 GNC - 3'
KoxII HgiJII GRGCYC GRGCY^C
C^RCGYG		 RGCY - 3'
KpnI GGTACC GGTAC^C
C^CATGG		 GTAC - 3'
Kpn2I BspMII TCCGGA T^CCGGA
AGGCC^T		 5' - GGCC
Kpn378I SacII CCGCGG CCGC^GG
GG^CGCC		 GC - 3'
Kpn2kI ScrFI CCNGG ^CCNGG
GGNCC^		 5' - GGNCC
Kpn49kI EcoRI GAATTC G^AATTC
CTTAA^G		 5' - TTAA
Kpn49kII CauII CCSGG ^CCSGG
GGSCC^		 5' - GGSCC
KspI SacII CCGCGG CCGC^GG
GG^CGCC		 GC - 3'
Ksp22I BclI TGATCA T^GATCA
ACTAG^T		 5' - CTAG
Ksp632I CTCTTC CTCTTCN^NNN
GAGAAGNNNN^		 5' - NNN
KspAI HpaI GTTAAC GTT^AAC
CAA^TTG		 Blunt
Kzo9I MboI GATC ^GATC
CTAG^		 5' - CTAG
Kzo49I AvaII GGWCC G^GWCC
CCWG^G		 5' - CWG