Enzymes Start with P

PCR Cloning

GenScript List of Common Restriction Enzyme Sites

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Enzyme	Prototype	Sequence	Cut Site	Overhang
PacI		TTAATTAA	TTAAT^TAA AAT^TAATT	AT - 3'
Pac25I	SmaI	CCCGGG	C^CCGGG GGGCC^C	5' - GGCC
PaeI	SphI	GCATGC	GCATG^C C^GTACG	CATG - 3'
PaeAI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
PaeBI	SmaI	CCCGGG	CCC^GGG GGG^CCC	Blunt
PaeHI	HgiJII	GRGCYC	GRGCY^C C^RCGYG	RGCY - 3'
PaePI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
PaeQI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
PaeR7I	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
Pae2kI	BglII	AGATCT	A^GATCT TCTAG^A	5' - CTAG
Pae5kI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
Pae14kI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
Pae17kI	PvuII	CAGCTG	CAG^CTG GTC^GAC	Blunt
Pae18kI	BglII	AGATCT	A^GATCT TCTAG^A	5' - CTAG
PagI	BspHI	TCATGA	T^CATGA AGTAC^T	5' - GTAC
PalI	HaeIII	GGCC	GG^CC CC^GG	Blunt
PamI	MstI	TGCGCA	TGC^GCA ACG^CGT	Blunt
PamII	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
PanI	XhoI	CTCGAG	C^TCGAG GAGCT^C	5' - AGCT
PasI		CCCWGGG	CC^CWGGG GGGWC^CC	5' - GWC
PauI	BsePI	GCGCGC	G^CGCGC CGCGC^G	5' - GCGC
PauAI	NspI	RCATGY	RCATG^Y R^GTACY	CATG - 3'
PauAII	AhaIII	TTTAAA	TTT^AAA AAA^TTT	Blunt
PceI	StuI	AGGCCT	AGG^CCT TCC^GGA	Blunt
PciI	BspLU11I	ACATGT	A^CATGT TGTAC^A	5' - GTAC
PctI	BsmI	GAATGC	GAATGCN^ CTTAC^GN	CN - 3'
Pde12I	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
Pde133I	HaeIII	GGCC	GG^CC CC^GG	Blunt
Pde137I	HpaII	CCGG	C^CGG GGC^C	5' - GC
PdiI	NaeI	GCCGGC	GCC^GGC CGG^CCG	Blunt
PdmI	XmnI	GAANNNNTTC	GAANN^NNTTC CTTNN^NNAAG	Blunt
PfaAI	HgiCI	GGYRCC	G^GYRCC CCYRG^G	5' - CYRG
PfaAII	NdeI	CATATG	CA^TATG GTAT^AC	5' - AT
PfaAIII	SphI	GCATGC	GCATG^C C^GTACG	CATG - 3'
PfeI	TfiI	GAWTC	G^AWTC CTWA^G	5' - TWA
Pfl8I	BamHI	GGATCC	G^GATCC CCTAG^G	5' - CTAG
Pfl21I	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
Pfl23II	SplI	CGTACG	C^GTACG GCATG^C	5' - CATG
Pfl27I	PpuMI	RGGWCCY	RG^GWCCY RCCWG^GY	5' - CWG
PflBI	PflMI	CCANNNNNTGG	CCANNNN^NTGG GGTN^NNNNACC	NNN - 3'
PflFI	Tth111I	GACNNNGTC	GACN^NNGTC CTGNN^NCAG	5' - N
PflKI	HaeIII	GGCC	GG^CC CC^GG	Blunt
PflMI		CCANNNNNTGG	CCANNNN^NTGG GGTN^NNNNACC	NNN - 3'
PfoI		TCCNGGA	T^CCNGGA AGGNCC^T	5' - GGNCC
PgaI	ClaI	ATCGAT	AT^CGAT TAGC^TA	5' - GC
PhaI	SfaNI	GCATC	GCATCNNNNN^NNNN CGTAGNNNNNNNNN^	5' - NNNN
PhoI	HaeIII	GGCC	GG^CC CC^GG	Blunt
PinAI	AgeI	ACCGGT	A^CCGGT TGGCC^A	5' - GGCC
PinBI	AvaIII	ATGCAT	ATGCA^T T^ACGTA	TGCA - 3'
PinBII	BspMII	TCCGGA	T^CCGGA AGGCC^T	5' - GGCC
PlaI	HaeIII	GGCC	GG^CC CC^GG	Blunt
PlaII	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
PlaAI	AvaI	CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
PlaAII	RsaI	GTAC	GT^AC CA^TG	Blunt
PleI		GAGTC	GAGTCNNNN^N CTCAGNNNNN^	5' - N
Ple19I	PvuI	CGATCG	CGAT^CG GC^TAGC	AT - 3'
PmaCI		CACGTG	CAC^GTG GTG^CAC	Blunt
PmeI		GTTTAAAC	GTTT^AAAC CAAA^TTTG	Blunt
Pme55I	StuI	AGGCCT	AGG^CCT TCC^GGA	Blunt
PmlI	PmaCI	CACGTG	CAC^GTG GTG^CAC	Blunt
PpaAI	AsuII	TTCGAA	TT^CGAA AAGC^TT	5' - GC
PpaAII	TaqI	TCGA	T^CGA AGC^T	5' - GC
PpeI	ApaI	GGGCCC	GGGCC^C C^CCGGG	GGCC - 3'
PpiI		GAACNNNNNCTC	GAACNNNNNCTCNNNNNNNNNNNNN^ CTTGNNNNNGAGNNNNNNNN^NNNNN	NNNNN - 3'
PpsI	PleI	GAGTC	GAGTCNNNN^N CTCAGNNNNN^	5' - N
Ppu10I	AvaIII	ATGCAT	A^TGCAT TACGT^A	5' - ACGT
Ppu111I	EcoRI	GAATTC	G^AATTC CTTAA^G	5' - TTAA
PpuAI	SplI	CGTACG	C^GTACG GCATG^C	5' - CATG
PpuMI		RGGWCCY	RG^GWCCY RCCWG^GY	5' - CWG
PpuXI	PpuMI	RGGWCCY	RG^GWCCY RCCWG^GY	5' - CWG
PshAI		GACNNNNGTC	GACNN^NNGTC CTGNN^NNCAG	Blunt
PshBI	VspI	ATTAAT	AT^TAAT TAAT^TA	5' - AT
PsiI		TTATAA	TTA^TAA AAT^ATT	Blunt
Psp03I	AvaII	GGWCC	GGWC^C C^CWGG	GWC - 3'
Psp5II	PpuMI	RGGWCCY	RG^GWCCY RCCWG^GY	5' - CWG
Psp6I	EcoRII	CCWGG	^CCWGG GGWCC^	5' - GGWCC
Psp23I	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
Psp1406I	AclI	AACGTT	AA^CGTT TTGC^AA	5' - GC
PspAI	SmaI	CCCGGG	C^CCGGG GGGCC^C	5' - GGCC
PspALI	SmaI	CCCGGG	CCC^GGG GGG^CCC	Blunt
Psp124BI	SacI	GAGCTC	GAGCT^C C^TCGAG	AGCT - 3'
PspCI	PmaCI	CACGTG	CAC^GTG GTG^CAC	Blunt
PspEI	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
PspGI	EcoRII	CCWGG	^CCWGG GGWCC^	5' - GGWCC
PspLI	SplI	CGTACG	C^GTACG GCATG^C	5' - CATG
PspN4I	NlaIV	GGNNCC	GGN^NCC CCN^NGG	Blunt
PspOMI	ApaI	GGGCCC	G^GGCCC CCCGG^G	5' - CCGG
PspPI	AsuI	GGNCC	G^GNCC CCNG^G	5' - CNG
PspPPI	PpuMI	RGGWCCY	RG^GWCCY RCCWG^GY	5' - CWG
PspXI		VCTCGAGB	VC^TCGAGB VGAGCT^CB	5' - AGCT
PsrI		GAACNNNNNNTAC	GAACNNNNNNTACNNNNNNNNNNNN^ CTTGNNNNNNATGNNNNNNN^NNNNN	NNNNN - 3'
PssI	DraII	RGGNCCY	RGGNC^CY RC^CNGGY	GNC - 3'
PstI		CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
PstNHI	NheI	GCTAGC	G^CTAGC CGATC^G	5' - GATC
PsuI	XhoII	RGATCY	R^GATCY RCTAG^Y	5' - CTAG
Psu161I	PvuI	CGATCG	CGAT^CG GC^TAGC	AT - 3'
PsuAI	BsaAI	YACGTR	YAC^GTR YTG^CAR	Blunt
PsyI	Tth111I	GACNNNGTC	GACN^NNGTC CTGNN^NCAG	5' - N
PtaI	BspMII	TCCGGA	T^CCGGA AGGCC^T	5' - GGCC
Pun14627I	MstI	TGCGCA	TGC^GCA ACG^CGT	Blunt
Pun14627II	PvuII	CAGCTG	CAG^CTG GTC^GAC	Blunt
PunAI	AvaI	CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
PunAII	NspI	RCATGY	RCATG^Y R^GTACY	CATG - 3'
PvuI		CGATCG	CGAT^CG GC^TAGC	AT - 3'
PvuII		CAGCTG	CAG^CTG GTC^GAC	Blunt
Pvu84II	PvuII	CAGCTG	CAG^CTG GTC^GAC	Blunt
PI-SceI		ATCTATGTCGGGTGC GGAGAAAGAGGTAAT GAAATGGCA	ATCTATGTCGGGTGC^GGAGAAAGAGGTAATGAAATGGCA TAGATACAGCC^CACGCCTCTTTCTCCATTACTTTACCGT	GTGC - 3'
PalAI	AscI	GGCGCGCC	GG^CGCGCC CCGCGC^GG	5' - GCGC
PciSI	SapI	GCTCTTC	GCTCTTCN^NNN CGAGAAGNNNN^	5' - NNN
PI-PspI		TGGCAAACAGCTATT ATGGGTATTATGGGT	TGGCAAACAGCTATTAT^GGGTATTATGGGT ACCGTTTGTCGAT^AATACCCATAATACCCA	TTAT - 3'
PluTI	NarI	GGCGCC	GGCGC^C C^CGCGG	GCGC - 3'
Ppu21I		YACGTR	YAC^GTR YTG^CAR	Blunt
PscI		ACATGT	A^CATGT TGTAC^A	5' - GTAC
PspFI		CCCAGC	CCCAG^C G^GGTCG	CCAG - 3'