** This list is collected to facilitate your research, and currently GenScript does not have restriction enzymes for sale.
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| Enzyme | Prototype | Sequence | Cut Site | Overhang |
|---|---|---|---|---|
| ItaI | Fnu4HI | GCNGC | GC^NGC CGN^CG |
5' - N |
| I-CeuI | TAACTATAACGGTCC TAAGGTAGCGA |
TAACTATAACGGTCCTAA^GGTAGCGA ATTGATATTGCCAG^GATTCCATCGCT |
CTAA - 3' | |
| I-PpoI | TAACTATGACTCTCT TAAGGTAGCCAAAT |
TAACTATGACT^CTCTTAAGGTAGCCAAAT ATTGATACTGAGAGAATT^CCATCGGTTTA |
5' - GAGAATT | |
| I-SceI | TAGGGATAACAGGGT AAT |
TAGGGATAA^CAGGGTAAT ATCCC^TATTGTCCCATTA |
ATAA - 3' |
