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PCR Cloning

GenScript List of Common Restriction Enzyme Sites

** This list is collected to facilitate your research, and currently GenScript does not have restriction enzymes for sale.

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Enzyme Prototype Sequence Cut Site Overhang
XagI EcoNI CCTNNNNNAGG CCTNN^NNNAGG
GGANNN^NNTCC		 5' - N
XapI ApoI RAATTY R^AATTY
RTTAA^Y		 5' - TTAA
XbaI TCTAGA T^CTAGA
AGATC^T		 5' - GATC
XcaI SnaI GTATAC GTA^TAC
CAT^ATG		 Blunt
XceI NspI RCATGY RCATG^Y
R^GTACY		 CATG - 3'
XciI SalI GTCGAC G^TCGAC
CAGCT^G		 5' - AGCT
XcmI CCANNNNNNNNNTGG CCANNNNN^NNNNTGG
GGTNNNN^NNNNNACC		 N - 3'
XcyI SmaI CCCGGG C^CCGGG
GGGCC^C		 5' - GGCC
XhoI CTCGAG C^TCGAG
GAGCT^C		 5' - AGCT
XhoII RGATCY R^GATCY
RCTAG^Y		 5' - CTAG
XmaI SmaI CCCGGG C^CCGGG
GGGCC^C		 5' - GGCC
XmaIII CGGCCG C^GGCCG
GCCGG^C		 5' - CCGG
XmaCI SmaI CCCGGG C^CCGGG
GGGCC^C		 5' - GGCC
XmaJI AvrII CCTAGG C^CTAGG
GGATC^C		 5' - GATC
XmiI AccI GTMKAC GT^MKAC
CAMK^TG		 5' - MK
XmnI GAANNNNTTC GAANN^NNTTC
CTTNN^NNAAG		 Blunt
XorII PvuI CGATCG CGAT^CG
GC^TAGC		 AT - 3'
XpaI XhoI CTCGAG C^TCGAG
GAGCT^C		 5' - AGCT
XspI MaeI CTAG C^TAG
GAT^C		 5' - AT