Enzymes Start with H

PCR Cloning

GenScript List of Common Restriction Enzyme Sites

** This list is collected to facilitate your research, and currently GenScript does not have restriction enzymes for sale.

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Enzyme	Prototype	Sequence	Cut Site	Overhang
HacI	MboI	GATC	^GATC CTAG^	5' - CTAG
HaeI		WGGCCW	WGG^CCW WCC^GGW	Blunt
HaeII		RGCGCY	RGCGC^Y R^CGCGY	GCGC - 3'
HaeIII		GGCC	GG^CC CC^GG	Blunt
HaeIV		GAYNNNNNRTC	GAYNNNNNRTCNNNNNNNNNNNNNN^ CTYNNNNNRAGNNNNNNNNN^NNNNN	NNNNN - 3'
HalI	EcoRI	GAATTC	G^AATTC CTTAA^G	5' - TTAA
HalII	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
HapII	HpaII	CCGG	C^CGG GGC^C	5' - GC
HgaI		GACGC	GACGCNNNNN^NNNNN CTGCGNNNNNNNNNN^	5' - NNNNN
HgiI	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
HgiAI		GWGCWC	GWGCW^C C^WCGWG	WGCW - 3'
HgiBI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
HgiCI		GGYRCC	G^GYRCC CCYRG^G	5' - CYRG
HgiCII	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
HgiCIII	SalI	GTCGAC	G^TCGAC CAGCT^G	5' - AGCT
HgiDI	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
HgiDII	SalI	GTCGAC	G^TCGAC CAGCT^G	5' - AGCT
HgiEI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
HgiGI	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
HgiHI	HgiCI	GGYRCC	G^GYRCC CCYRG^G	5' - CYRG
HgiHII	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
HgiHIII	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
HgiJI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
HgiJII		GRGCYC	GRGCY^C C^RCGYG	RGCY - 3'
HgiS22I	CauII	CCSGG	CC^SGG GGS^CC	5' - S
HhaI		GCGC	GCG^C C^GCG	CG - 3'
HhaII	HinfI	GANTC	G^ANTC CTNA^G	5' - TNA
Hin1I	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
Hin1II	NlaIII	CATG	CATG^ ^GTAC	CATG - 3'
Hin2I	HpaII	CCGG	C^CGG GGC^C	5' - GC
Hin4I		GAYNNNNNVTC	GAYNNNNNVTCNNNNNNNNNNNNN^ CTYNNNNNVAGNNNNNNNN^NNNNN	NNNNN - 3'
Hin6I	HhaI	GCGC	G^CGC CGC^G	5' - GC
HinJCI	HindII	GTYRAC	GTY^RAC CAY^RTG	Blunt
HinP1I	HhaI	GCGC	G^CGC CGC^G	5' - GC
HincII	HindII	GTYRAC	GTY^RAC CAY^RTG	Blunt
HindII		GTYRAC	GTY^RAC CAY^RTG	Blunt
HindIII		AAGCTT	A^AGCTT TTCGA^A	5' - TCGA
HinfI		GANTC	G^ANTC CTNA^G	5' - TNA
HjaI	EcoRV	GATATC	GAT^ATC CTA^TAG	Blunt
HpaI		GTTAAC	GTT^AAC CAA^TTG	Blunt
HpaII		CCGG	C^CGG GGC^C	5' - GC
HphI		GGTGA	GGTGANNNNNNNN^ CCACTNNNNNNN^N	N - 3'
Hpy8I	MjaIV	GTNNAC	GTN^NAC CAN^NTG	Blunt
Hpy51I	Tsp45I	GTSAC	^GTSAC CASTG^	5' - CASTG
Hpy99I		CGWCG	CGWCG^ ^GCWGC	CGWCG - 3'
Hpy178III		TCNNGA	TC^NNGA AGNN^CT	5' - NN
Hpy188I		TCNGA	TCN^GA AG^NCT	N - 3'
Hpy188III	Hpy178III	TCNNGA	TC^NNGA AGNN^CT	5' - NN
HpyAV	Hin4II	CCTTC	CCTTCNNNNNN^ GGAAGNNNNN^N	N - 3'
HpyBI	RsaI	GTAC	GT^AC CA^TG	Blunt
HpyBII	MjaIV	GTNNAC	GTN^NAC CAN^NTG	Blunt
HpyCI	EcoRV	GATATC	GAT^ATC CTA^TAG	Blunt
HpyC1I	BccI	CCATC	CCATCNNNN^N GGTAGNNNNN^	5' - N
HpyCH4I	NlaIII	CATG	CATG^ ^GTAC	CATG - 3'
HpyCH4III	Tsp4CI	ACNGT	ACN^GT TG^NCA	N - 3'
HpyCH4IV	MaeII	ACGT	A^CGT TGC^A	5' - GC
HpyCH4V	CviRI	TGCA	TG^CA AC^GT	Blunt
HpyF10VI	MwoI	GCNNNNNNNGC	GCNNNNN^NNGC CGNN^NNNNNCG	NNN - 3'
HpyF44III	CviRI	TGCA	TG^CA AC^GT	Blunt
HsoI	HhaI	GCGC	G^CGC CGC^G	5' - GC
Hsp92I	AcyI	GRCGYC	GR^CGYC CRGC^YG	5' - GC
Hsp92II	NlaIII	CATG	CATG^ ^GTAC	CATG - 3'
HspAI	HhaI	GCGC	G^CGC CGC^G	5' - GC
HsuI	HindIII	AAGCTT	A^AGCTT TTCGA^A	5' - TCGA
Hpy166II	MjaIV	GTNNAC	GTN^NAC CAN^NTG	Blunt
HpyF3I	DdeI	CTNAG	C^TNAG GANT^C	5' - ANT