GenScript List of Common Restriction Enzyme Sites

** This list is collected to facilitate your research, and currently GenScript does not have restriction enzymes for sale.

ALL

A B C D E F G H I K L M N O P R S T U V X Y Z

Sort by name start with H

Enzyme Prototype Sequence Cut Site Overhang
HacI MboI GATC ^GATC
CTAG^
5' - CTAG
HaeI WGGCCW WGG^CCW
WCC^GGW
Blunt
HaeII RGCGCY RGCGC^Y
R^CGCGY
GCGC - 3'
HaeIII GGCC GG^CC
CC^GG
Blunt
HaeIV GAYNNNNNRTC GAYNNNNNRTCNNNNNNNNNNNNNN^
CTYNNNNNRAGNNNNNNNNN^NNNNN
NNNNN - 3'
HalI EcoRI GAATTC G^AATTC
CTTAA^G
5' - TTAA
HalII PstI CTGCAG CTGCA^G
G^ACGTC
TGCA - 3'
HapII HpaII CCGG C^CGG
GGC^C
5' - GC
HgaI GACGC GACGCNNNNN^NNNNN
CTGCGNNNNNNNNNN^
5' - NNNNN
HgiI AcyI GRCGYC GR^CGYC
CRGC^YG
5' - GC
HgiAI GWGCWC GWGCW^C
C^WCGWG
WGCW - 3'
HgiBI AvaII GGWCC G^GWCC
CCWG^G
5' - CWG
HgiCI GGYRCC G^GYRCC
CCYRG^G
5' - CYRG
HgiCII AvaII GGWCC G^GWCC
CCWG^G
5' - CWG
HgiCIII SalI GTCGAC G^TCGAC
CAGCT^G
5' - AGCT
HgiDI AcyI GRCGYC GR^CGYC
CRGC^YG
5' - GC
HgiDII SalI GTCGAC G^TCGAC
CAGCT^G
5' - AGCT
HgiEI AvaII GGWCC G^GWCC
CCWG^G
5' - CWG
HgiGI AcyI GRCGYC GR^CGYC
CRGC^YG
5' - GC
HgiHI HgiCI GGYRCC G^GYRCC
CCYRG^G
5' - CYRG
HgiHII AcyI GRCGYC GR^CGYC
CRGC^YG
5' - GC
HgiHIII AvaII GGWCC G^GWCC
CCWG^G
5' - CWG
HgiJI AvaII GGWCC G^GWCC
CCWG^G
5' - CWG
HgiJII GRGCYC GRGCY^C
C^RCGYG
RGCY - 3'
HgiS22I CauII CCSGG CC^SGG
GGS^CC
5' - S
HhaI GCGC GCG^C
C^GCG
CG - 3'
HhaII HinfI GANTC G^ANTC
CTNA^G
5' - TNA
Hin1I AcyI GRCGYC GR^CGYC
CRGC^YG
5' - GC
Hin1II NlaIII CATG CATG^
^GTAC
CATG - 3'
Hin2I HpaII CCGG C^CGG
GGC^C
5' - GC
Hin4I GAYNNNNNVTC GAYNNNNNVTCNNNNNNNNNNNNN^
CTYNNNNNVAGNNNNNNNN^NNNNN
NNNNN - 3'
Hin6I HhaI GCGC G^CGC
CGC^G
5' - GC
HinJCI HindII GTYRAC GTY^RAC
CAY^RTG
Blunt
HinP1I HhaI GCGC G^CGC
CGC^G
5' - GC
HincII HindII GTYRAC GTY^RAC
CAY^RTG
Blunt
HindII GTYRAC GTY^RAC
CAY^RTG
Blunt
HindIII AAGCTT A^AGCTT
TTCGA^A
5' - TCGA
HinfI GANTC G^ANTC
CTNA^G
5' - TNA
HjaI EcoRV GATATC GAT^ATC
CTA^TAG
Blunt
HpaI GTTAAC GTT^AAC
CAA^TTG
Blunt
HpaII CCGG C^CGG
GGC^C
5' - GC
HphI GGTGA GGTGANNNNNNNN^
CCACTNNNNNNN^N
N - 3'
Hpy8I MjaIV GTNNAC GTN^NAC
CAN^NTG
Blunt
Hpy51I Tsp45I GTSAC ^GTSAC
CASTG^
5' - CASTG
Hpy99I CGWCG CGWCG^
^GCWGC
CGWCG - 3'
Hpy178III TCNNGA TC^NNGA
AGNN^CT
5' - NN
Hpy188I TCNGA TCN^GA
AG^NCT
N - 3'
Hpy188III Hpy178III TCNNGA TC^NNGA
AGNN^CT
5' - NN
HpyAV Hin4II CCTTC CCTTCNNNNNN^
GGAAGNNNNN^N
N - 3'
HpyBI RsaI GTAC GT^AC
CA^TG
Blunt
HpyBII MjaIV GTNNAC GTN^NAC
CAN^NTG
Blunt
HpyCI EcoRV GATATC GAT^ATC
CTA^TAG
Blunt
HpyC1I BccI CCATC CCATCNNNN^N
GGTAGNNNNN^
5' - N
HpyCH4I NlaIII CATG CATG^
^GTAC
CATG - 3'
HpyCH4III Tsp4CI ACNGT ACN^GT
TG^NCA
N - 3'
HpyCH4IV MaeII ACGT A^CGT
TGC^A
5' - GC
HpyCH4V CviRI TGCA TG^CA
AC^GT
Blunt
HpyF10VI MwoI GCNNNNNNNGC GCNNNNN^NNGC
CGNN^NNNNNCG
NNN - 3'
HpyF44III CviRI TGCA TG^CA
AC^GT
Blunt
HsoI HhaI GCGC G^CGC
CGC^G
5' - GC
Hsp92I AcyI GRCGYC GR^CGYC
CRGC^YG
5' - GC
Hsp92II NlaIII CATG CATG^
^GTAC
CATG - 3'
HspAI HhaI GCGC G^CGC
CGC^G
5' - GC
HsuI HindIII AAGCTT A^AGCTT
TTCGA^A
5' - TCGA
Hpy166II MjaIV GTNNAC GTN^NAC
CAN^NTG
Blunt
HpyF3I DdeI CTNAG C^TNAG
GANT^C
5' - ANT