Enzymes Start with E

PCR Cloning

GenScript List of Common Restriction Enzyme Sites

** This list is collected to facilitate your research, and currently GenScript does not have restriction enzymes for sale.

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Enzyme	Prototype	Sequence	Cut Site	Overhang
EacI	BinI	GGATC	GGATCNNNN^N CCTAGNNNNN^	5' - N
EaeI	CfrI	YGGCCR	Y^GGCCR YCCGG^R	5' - CCGG
Eae46I	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
EaeAI	SmaI	CCCGGG	C^CCGGG GGGCC^C	5' - GGCC
EagI	XmaIII	CGGCCG	C^GGCCG GCCGG^C	5' - CCGG
EagBI	PvuI	CGATCG	CGAT^CG GC^TAGC	AT - 3'
EagMI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
Eam1104I	Ksp632I	CTCTTC	CTCTTCN^NNN GAGAAGNNNN^	5' - NNN
Eam1105I		GACNNNNNGTC	GACNNN^NNGTC CTGNN^NNNCAG	N - 3'
EarI	Ksp632I	CTCTTC	CTCTTCN^NNN GAGAAGNNNN^	5' - NNN
EcaI	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
EciI		GGCGGA	GGCGGANNNNNNNNNNN^ CCGCCTNNNNNNNNN^NN	NN - 3'
Eci125I	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
EclI	PvuII	CAGCTG	CAG^CTG GTC^GAC	Blunt
Ecl136II	SacI	GAGCTC	GAG^CTC CTC^GAG	Blunt
EclHKI	Eam1105I	GACNNNNNGTC	GACNNN^NNGTC CTGNN^NNNCAG	N - 3'
EclRI	SmaI	CCCGGG	C^CCGGG GGGCC^C	5' - GGCC
EclXI	XmaIII	CGGCCG	C^GGCCG GCCGG^C	5' - CCGG
Ecl18kI	ScrFI	CCNGG	^CCNGG GGNCC^	5' - GGNCC
Ecl37kI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
Ecl2zI	PstI	CTGCAG	CTGCA^G G^ACGTC	TGCA - 3'
Eco24I	HgiJII	GRGCYC	GRGCY^C C^RCGYG	RGCY - 3'
Eco31I		GGTCTC	GGTCTCN^NNNN CCAGAGNNNNN^	5' - NNNN
Eco32I	EcoRV	GATATC	GAT^ATC CTA^TAG	Blunt
Eco47I	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
Eco47III		AGCGCT	AGC^GCT TCG^CGA	Blunt
Eco52I	XmaIII	CGGCCG	C^GGCCG GCCGG^C	5' - CCGG
Eco56I	NaeI	GCCGGC	G^CCGGC CGGCC^G	5' - GGCC
Eco57I		CTGAAG	CTGAAGNNNNNNNNNNNNNNNN^ GACTTCNNNNNNNNNNNNNN^NN	NN - 3'
Eco64I	HgiCI	GGYRCC	G^GYRCC CCYRG^G	5' - CYRG
Eco72I	PmaCI	CACGTG	CAC^GTG GTG^CAC	Blunt
Eco78I	NarI	GGCGCC	GGC^GCC CCG^CGG	Blunt
Eco81I	SauI	CCTNAGG	CC^TNAGG GGANT^CC	5' - ANT
Eco88I	AvaI	CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
Eco91I	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
Eco105I	SnaBI	TACGTA	TAC^GTA ATG^CAT	Blunt
Eco130I	StyI	CCWWGG	C^CWWGG GGWWC^C	5' - GWWC
Eco147I	StuI	AGGCCT	AGG^CCT TCC^GGA	Blunt
Eco255I	ScaI	AGTACT	AGT^ACT TCA^TGA	Blunt
Eco1831I	CauII	CCSGG	^CCSGG GGSCC^	5' - GGSCC
EcoA4I	Eco31I	GGTCTC	GGTCTCN^NNNN CCAGAGNNNNN^	5' - NNNN
EcoHI	CauII	CCSGG	^CCSGG GGSCC^	5' - GGSCC
EcoHK31I	CfrI	YGGCCR	Y^GGCCR YCCGG^R	5' - CCGG
EcoICRI	SacI	GAGCTC	GAG^CTC CTC^GAG	Blunt
Eco75KI	HgiJII	GRGCYC	GRGCY^C C^RCGYG	RGCY - 3'
Eco57MI		CTGRAG	CTGRAGNNNNNNNNNNNNNNNN^ GACRTCNNNNNNNNNNNNNN^NN	NN - 3'
EcoNI		CCTNNNNNAGG	CCTNN^NNNAGG GGANNN^NNTCC	5' - N
EcoO44I	Eco31I	GGTCTC	GGTCTCN^NNNN CCAGAGNNNNN^	5' - NNNN
EcoO65I	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
EcoO109I	DraII	RGGNCCY	RG^GNCCY RCCNG^GY	5' - CNG
EcoO128I	BstEII	GGTNACC	G^GTNACC CCANTG^G	5' - CANTG
EcoRI		GAATTC	G^AATTC CTTAA^G	5' - TTAA
EcoRII		CCWGG	^CCWGG GGWCC^	5' - GGWCC
EcoRV		GATATC	GAT^ATC CTA^TAG	Blunt
EcoT14I	StyI	CCWWGG	C^CWWGG GGWWC^C	5' - GWWC
EcoT22I	AvaIII	ATGCAT	ATGCA^T T^ACGTA	TGCA - 3'
EcoT38I	HgiJII	GRGCYC	GRGCY^C C^RCGYG	RGCY - 3'
EcoVIII	HindIII	AAGCTT	A^AGCTT TTCGA^A	5' - TCGA
Eco13kI	ScrFI	CCNGG	^CCNGG GGNCC^	5' - GGNCC
Eco21kI	ScrFI	CCNGG	^CCNGG GGNCC^	5' - GGNCC
Eco27kI	AvaI	CYCGRG	C^YCGRG GYGCR^C	5' - YGCR
Eco29kI	SacII	CCGCGG	CCGC^GG GG^CGCC	GC - 3'
Eco53kI	SacI	GAGCTC	GAG^CTC CTC^GAG	Blunt
Eco137kI	ScrFI	CCNGG	^CCNGG GGNCC^	5' - GGNCC
EgeI	NarI	GGCGCC	GGC^GCC CCG^CGG	Blunt
EheI	NarI	GGCGCC	GGC^GCC CCG^CGG	Blunt
ErhI	StyI	CCWWGG	C^CWWGG GGWWC^C	5' - GWWC
ErhB9I	PvuI	CGATCG	CGAT^CG GC^TAGC	AT - 3'
ErhB9II	StyI	CCWWGG	C^CWWGG GGWWC^C	5' - GWWC
ErpI	AvaII	GGWCC	G^GWCC CCWG^G	5' - CWG
EsaBC3I	TaqI	TCGA	TC^GA AG^CT	Blunt
EsaBC4I	HaeIII	GGCC	GG^CC CC^GG	Blunt
EspI		GCTNAGC	GC^TNAGC CGANT^CG	5' - ANT
Esp3I		CGTCTC	CGTCTCN^NNNN GCAGAGNNNNN^	5' - NNNN
Esp4I	AflII	CTTAAG	C^TTAAG GAATT^C	5' - AATT
Esp1396I	PflMI	CCANNNNNTGG	CCANNNN^NTGG GGTN^NNNNACC	NNN - 3'
EcoP15I		CAGCAG	CAGCAGNNNNNNNNNNNNNNNNNNNNNNNNN^NN GTCGTCNNNNNNNNNNNNNNNNNNNNNNNNNNN^	5' - NN