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PCR Cloning

GenScript List of Common Restriction Enzyme Sites

** This list is collected to facilitate your research, and currently GenScript does not have restriction enzymes for sale.

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Enzyme Prototype Sequence Cut Site Overhang
Bac36I AsuI GGNCC G^GNCC
CCNG^G		 5' - CNG
BaeI ACNNNNGTAYC ACNNNNGTAYCNNNNNNNNNNNN^
TGNNNNCATYGNNNNNNN^NNNNN		 NNNNN - 3'
BalI TGGCCA TGG^CCA
ACC^GGT		 Blunt
Bal228I AsuI GGNCC G^GNCC
CCNG^G		 5' - CNG
BamHI GGATCC G^GATCC
CCTAG^G		 5' - CTAG
BamNxI AvaII GGWCC G^GWCC
CCWG^G		 5' - CWG
BanI HgiCI GGYRCC G^GYRCC
CCYRG^G		 5' - CYRG
BanII HgiJII GRGCYC GRGCY^C
C^RCGYG		 RGCY - 3'
BanIII ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
BanAI HaeIII GGCC GG^CC
CC^GG		 Blunt
BasI PflMI CCANNNNNTGG CCANNNN^NTGG
GGTN^NNNNACC		 NNN - 3'
BauI BsiI CACGAG C^ACGAG
GTGCT^C		 5' - TGCT
BavI PvuII CAGCTG CAG^CTG
GTC^GAC		 Blunt
BavAI PvuII CAGCTG CAG^CTG
GTC^GAC		 Blunt
BavAII AsuI GGNCC G^GNCC
CCNG^G		 5' - CNG
BavBI PvuII CAGCTG CAG^CTG
GTC^GAC		 Blunt
BavBII AsuI GGNCC G^GNCC
CCNG^G		 5' - CNG
BavCI ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
BbeI NarI GGCGCC GGCGC^C
C^CGCGG		 GCGC - 3'
BbiII AcyI GRCGYC GR^CGYC
CRGC^YG		 5' - GC
Bbi24I MluI ACGCGT A^CGCGT
TGCGC^A		 5' - GCGC
BbrI HindIII AAGCTT A^AGCTT
TTCGA^A		 5' - TCGA
Bbr7I BbvII GAAGAC GAAGACNNNNNNN^NNNN
CTTCTGNNNNNNNNNNN^		 5' - NNNN
BbrPI PmaCI CACGTG CAC^GTG
GTG^CAC		 Blunt
BbsI BbvII GAAGAC GAAGACNN^NNNN
CTTCTGNNNNNN^		 5' - NNNN
BbuI SphI GCATGC GCATG^C
C^GTACG		 CATG - 3'
BbvI GCAGC GCAGCNNNNNNNN^NNNN
CGTCGNNNNNNNNNNNN^		 5' - NNNN
BbvII GAAGAC GAAGACNN^NNNN
CTTCTGNNNNNN^		 5' - NNNN
Bbv12I HgiAI GWGCWC GWGCW^C
C^WCGWG		 WGCW - 3'
Bbv16II BbvII GAAGAC GAAGACNN^NNNN
CTTCTGNNNNNN^		 5' - NNNN
BbvAI XmnI GAANNNNTTC GAANN^NNTTC
CTTNN^NNAAG		 Blunt
BbvAII ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
BbvAIII BspMII TCCGGA T^CCGGA
AGGCC^T		 5' - GGCC
BbvBI HgiCI GGYRCC G^GYRCC
CCYRG^G		 5' - CYRG
BbvCI CCTCAGC CC^TCAGC
GGAGT^CG		 5' - AGT
Bca77I BetI WCCGGW W^CCGGW
WGGCC^W		 5' - GGCC
BccI CCATC CCATCNNNN^N
GGTAGNNNNN^		 5' - N
Bce4I MwoI GCNNNNNNNGC GCNNNNN^NNGC
CGNN^NNNNNCG		 NNN - 3'
Bce22I AsuI GGNCC G^GNCC
CCNG^G		 5' - CNG
Bce83I CTTGAG CTTGAGNNNNNNNNNNNNNNNN^
GAACTCNNNNNNNNNNNNNN^NN		 NN - 3'
Bce243I MboI GATC ^GATC
CTAG^		 5' - CTAG
Bce751I BamHI GGATCC G^GATCC
CCTAG^G		 5' - CTAG
BceAI BcefI ACGGC ACGGCNNNNNNNNNNNN^NN
TGCCGNNNNNNNNNNNNNN^		 5' - NN
BceBI FnuDII CGCG CG^CG
GC^GC		 Blunt
BceCI MwoI GCNNNNNNNGC GCNNNNN^NNGC
CGNN^NNNNNCG		 NNN - 3'
BcefI ACGGC ACGGCNNNNNNNNNNNN^N
TGCCGNNNNNNNNNNNNN^		 5' - N
BcgI CGANNNNNNTGC CGANNNNNNTGCNNNNNNNNNNNN^
GCTNNNNNNACGNNNNNNNNNN^NN		 NN - 3'
Bci29I ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
BciBI ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
BciBII EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
BciVI GTATCC GTATCCNNNNNN^
CATAGGNNNNN^N		 N - 3'
BclI TGATCA T^GATCA
ACTAG^T		 5' - CTAG
BcmI ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
BcnI CauII CCSGG CC^SGG
GGS^CC		 5' - S
BcoI AvaI CYCGRG C^YCGRG
GYGCR^C		 5' - YGCR
Bco5I Ksp632I CTCTTC CTCTTCN^NNN
GAGAAGNNNN^		 5' - NNN
Bco27I HpaII CCGG C^CGG
GGC^C		 5' - GC
Bco116I Ksp632I CTCTTC CTCTTCN^NNN
GAGAAGNNNN^		 5' - NNN
Bco118I Cfr10I RCCGGY R^CCGGY
RGGCC^Y		 5' - GGCC
BcoAI PmaCI CACGTG CAC^GTG
GTG^CAC		 Blunt
BcoKI Ksp632I CTCTTC CTCTTCN^NNN
GAGAAGNNNN^		 5' - NNN
BcuI SpeI ACTAGT A^CTAGT
TGATC^A		 5' - GATC
BcuAI AvaII GGWCC G^GWCC
CCWG^G		 5' - CWG
BdiI ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
BdiSI SfeI CTRYAG C^TRYAG
GARYT^C		 5' - ARYT
BecAII HaeIII GGCC GG^CC
CC^GG		 Blunt
BepI FnuDII CGCG CG^CG
GC^GC		 Blunt
BetI WCCGGW W^CCGGW
WGGCC^W		 5' - GGCC
BfaI MaeI CTAG C^TAG
GAT^C		 5' - AT
BfiI ACTGGG ACTGGGNNNNN^
TGACCCNNNN^N		 N - 3'
Bfi57I MboI GATC ^GATC
CTAG^		 5' - CTAG
Bfi89I CfrI YGGCCR Y^GGCCR
YCCGG^R		 5' - CCGG
BflI BsiYI CCNNNNNNNGG CCNNNNN^NNGG
GGNN^NNNNNCC		 NNN - 3'
BfmI SfeI CTRYAG C^TRYAG
GARYT^C		 5' - ARYT
BfrI AflII CTTAAG C^TTAAG
GAATT^C		 5' - AATT
BfrBI AvaIII ATGCAT ATG^CAT
TAC^GTA		 Blunt
BfuI BciVI GTATCC GTATCCNNNNNN^
CATAGGNNNNN^N		 N - 3'
BfuAI BspMI ACCTGC ACCTGCNNNN^NNNN
TGGACGNNNNNNNN^		 5' - NNNN
BfuCI MboI GATC ^GATC
CTAG^		 5' - CTAG
BglI GCCNNNNNGGC GCCNNNN^NGGC
CGGN^NNNNCCG		 NNN - 3'
BglII AGATCT A^GATCT
TCTAG^A		 5' - CTAG
BimI AsuII TTCGAA TT^CGAA
AAGC^TT		 5' - GC
Bim19I AsuII TTCGAA TT^CGAA
AAGC^TT		 5' - GC
Bim19II HaeIII GGCC GG^CC
CC^GG		 Blunt
BinI GGATC GGATCNNNN^N
CCTAGNNNNN^		 5' - N
BlfI BspMII TCCGGA T^CCGGA
AGGCC^T		 5' - GGCC
Bli41I ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
Bli86I ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
Bli736I Eco31I GGTCTC GGTCTCN^NNNN
CCAGAGNNNNN^		 5' - NNNN
BliAI ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
BliHKI SauI CCTNAGG CC^TNAGG
GGANT^CC		 5' - ANT
BliRI ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
BlnI AvrII CCTAGG C^CTAGG
GGATC^C		 5' - GATC
BloHI XhoII RGATCY R^GATCY
RCTAG^Y		 5' - CTAG
BloHII PstI CTGCAG CTGCA^G
G^ACGTC		 TGCA - 3'
BlpI EspI GCTNAGC GC^TNAGC
CGANT^CG		 5' - ANT
BluI XhoI CTCGAG C^TCGAG
GAGCT^C		 5' - AGCT
Bme12I MboI GATC ^GATC
CTAG^		 5' - CTAG
Bme18I AvaII GGWCC G^GWCC
CCWG^G		 5' - CWG
Bme142I HaeII RGCGCY RGC^GCY
RCG^CGY		 Blunt
Bme216I AvaII GGWCC G^GWCC
CCWG^G		 5' - CWG
Bme361I HaeIII GGCC GG^CC
CC^GG		 Blunt
Bme585I FauI CCCGC CCCGCNNNN^NN
GGGCGNNNNNN^		 5' - NN
Bme1390I ScrFI CCNGG CC^NGG
GGN^CC		 5' - N
Bme1580I BseSI GKGCMC GKGCM^C
C^KCGMG		 KGCM - 3'
BmgBI BtrI CACGTC CAC^GTC
GTG^CAG		 Blunt
BmrI BfiI ACTGGG ACTGGGNNNNN^
TGACCCNNNN^N		 N - 3'
BmtI NheI GCTAGC GCTAG^C
C^GATCG		 CTAG - 3'
BmyI SduI GDGCHC GDGCH^C
C^DCGHG		 DGCH - 3'
BnaI BamHI GGATCC G^GATCC
CCTAG^G		 5' - CTAG
BoxI PshAI GACNNNNGTC GACNN^NNGTC
CTGNN^NNCAG		 Blunt
BpcI SfeI CTRYAG C^TRYAG
GARYT^C		 5' - ARYT
BpiI BbvII GAAGAC GAAGACNN^NNNN
CTTCTGNNNNNN^		 5' - NNNN
BplI GAGNNNNNCTC GAGNNNNNCTCNNNNNNNNNNNNN^
CTCNNNNNGAGNNNNNNNN^NNNNN		 NNNNN - 3'
BpmI GsuI CTGGAG CTGGAGNNNNNNNNNNNNNNNN^
GACCTCNNNNNNNNNNNNNN^NN		 NN - 3'
BpoAI VspI ATTAAT AT^TAAT
TAAT^TA		 5' - AT
BptI EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
BpuI HgiJII GRGCYC GRGCY^C
C^RCGYG		 RGCY - 3'
Bpu10I CCTNAGC CC^TNAGC
GGANT^CG		 5' - ANT
Bpu14I AsuII TTCGAA TT^CGAA
AAGC^TT		 5' - GC
Bpu95I FnuDII CGCG CG^CG
GC^GC		 Blunt
Bpu1102I EspI GCTNAGC GC^TNAGC
CGANT^CG		 5' - ANT
BpuAI BbvII GAAGAC GAAGACNN^NNNN
CTTCTGNNNNNN^		 5' - NNNN
BpuAmI SacI GAGCTC GAG^CTC
CTC^GAG		 Blunt
BpuB5I SplI CGTACG C^GTACG
GCATG^C		 5' - CATG
BpuDI Bpu10I CCTNAGC CC^TNAGC
GGANT^CG		 5' - ANT
BpuEI Bce83I CTTGAG CTTGAGNNNNNNNNNNNNNNNN^
GAACTCNNNNNNNNNNNNNN^NN		 NN - 3'
BpuSI FinI GGGAC GGGACNNNNNNNNNN^NNNN
CCCTGNNNNNNNNNNNNNN^		 5' - NNNN
BsaI Eco31I GGTCTC GGTCTCN^NNNN
CCAGAGNNNNN^		 5' - NNNN
Bsa29I ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
BsaAI YACGTR YAC^GTR
YTG^CAR		 Blunt
BsaBI GATNNNNATC GATNN^NNATC
CTANN^NNTAG		 Blunt
BsaHI AcyI GRCGYC GR^CGYC
CRGC^YG		 5' - GC
BsaJI SecI CCNNGG C^CNNGG
GGNNC^C		 5' - GNNC
BsaMI BsmI GAATGC GAATGCN^
CTTAC^GN		 CN - 3'
BsaOI McrI CGRYCG CGRY^CG
GC^RYGC		 RY - 3'
BsaWI BetI WCCGGW W^CCGGW
WGGCC^W		 5' - GGCC
BsaXI ACNNNNNCTCC ACNNNNNCTCCNNNNNNNNNN^
TGNNNNNGAGGNNNNNNN^NNN		 NNN - 3'
BscI ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
Bsc4I BsiYI CCNNNNNNNGG CCNNNNN^NNGG
GGNN^NNNNNCC		 NNN - 3'
Bsc91I BbvII GAAGAC GAAGACNN^NNNN
CTTCTGNNNNNN^		 5' - NNNN
Bsc107I BsiYI CCNNNNNNNGG CCNNNNN^NNGG
GGNN^NNNNNCC		 NNN - 3'
BscAI SfaNI GCATC GCATCNNNN^NN
CGTAGNNNNNN^		 5' - NN
BscBI NlaIV GGNNCC GGN^NCC
CCN^NGG		 Blunt
BscCI BsmI GAATGC GAATGCN^
CTTAC^GN		 CN - 3'
BscFI MboI GATC ^GATC
CTAG^		 5' - CTAG
Bse1I BsrI ACTGG ACTGGN^
TGAC^CN		 GN - 3'
Bse8I BsaBI GATNNNNATC GATNN^NNATC
CTANN^NNTAG		 Blunt
Bse15I AvaI CYCGRG C^YCGRG
GYGCR^C		 5' - YGCR
Bse16I EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
Bse17I EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
Bse21I SauI CCTNAGG CC^TNAGG
GGANT^CC		 5' - ANT
Bse24I EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
Bse64I BstEII GGTNACC G^GTNACC
CCANTG^G		 5' - CANTG
Bse118I Cfr10I RCCGGY R^CCGGY
RGGCC^Y		 5' - GGCC
Bse634I Cfr10I RCCGGY R^CCGGY
RGGCC^Y		 5' - GGCC
BseAI BspMII TCCGGA T^CCGGA
AGGCC^T		 5' - GGCC
BseBI EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
BseCI ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
BseDI SecI CCNNGG C^CNNGG
GGNNC^C		 5' - GNNC
Bse3DI BsrDI GCAATG GCAATGNN^
CGTTAC^NN		 NN - 3'
BseGI FokI GGATG GGATGNN^
CCTAC^NN		 NN - 3'
BseJI BsaBI GATNNNNATC GATNN^NNATC
CTANN^NNTAG		 Blunt
BseKI BbvI GCAGC GCAGCNNNNNNNN^NNNN
CGTCGNNNNNNNNNNNN^		 5' - NNNN
BseLI BsiYI CCNNNNNNNGG CCNNNNN^NNGG
GGNN^NNNNNCC		 NNN - 3'
BseMI BsrDI GCAATG GCAATGNN^
CGTTAC^NN		 NN - 3'
BseMII CTCAG CTCAGNNNNNNNNNN^
GAGTCNNNNNNNN^NN		 NN - 3'
BseNI BsrI ACTGG ACTGGN^
TGAC^CN		 GN - 3'
BsePI GCGCGC G^CGCGC
CGCGC^G		 5' - GCGC
BseQI HaeIII GGCC GG^CC
CC^GG		 Blunt
BseRI GAGGAG GAGGAGNNNNNNNNNN^
CTCCTCNNNNNNNN^NN		 NN - 3'
BseSI GKGCMC GKGCM^C
C^KCGMG		 KGCM - 3'
BseT9I BstEII GGTNACC G^GTNACC
CCANTG^G		 5' - CANTG
BseT10I BstEII GGTNACC G^GTNACC
CCANTG^G		 5' - CANTG
BseXI BbvI GCAGC GCAGCNNNNNNNN^NNNN
CGTCGNNNNNNNNNNNN^		 5' - NNNN
BseX3I XmaIII CGGCCG C^GGCCG
GCCGG^C		 5' - CCGG
BseYI CCCAGC C^CCAGC
GGGTC^G		 5' - GGTC
BseZI Ksp632I CTCTTC CTCTTCN^NNN
GAGAAGNNNN^		 5' - NNN
BsgI GTGCAG GTGCAGNNNNNNNNNNNNNNNN^
CACGTCNNNNNNNNNNNNNN^NN		 NN - 3'
BshI HaeIII GGCC GG^CC
CC^GG		 Blunt
Bsh45I HgiAI GWGCWC GWGCW^C
C^WCGWG		 WGCW - 3'
Bsh1236I FnuDII CGCG CG^CG
GC^GC		 Blunt
Bsh1285I McrI CGRYCG CGRY^CG
GC^RYGC		 RY - 3'
Bsh1365I BsaBI GATNNNNATC GATNN^NNATC
CTANN^NNTAG		 Blunt
BshFI HaeIII GGCC GG^CC
CC^GG		 Blunt
BshGI EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
BshKI AsuI GGNCC G^GNCC
CCNG^G		 5' - CNG
BshNI HgiCI GGYRCC G^GYRCC
CCYRG^G		 5' - CYRG
BshTI AgeI ACCGGT A^CCGGT
TGGCC^A		 5' - GGCC
BsiI CACGAG C^ACGAG
GTGCT^C		 5' - TGCT
BsiBI BsaBI GATNNNNATC GATNN^NNATC
CTANN^NNTAG		 Blunt
BsiCI AsuII TTCGAA TT^CGAA
AAGC^TT		 5' - GC
BsiEI McrI CGRYCG CGRY^CG
GC^RYGC		 RY - 3'
BsiHKAI HgiAI GWGCWC GWGCW^C
C^WCGWG		 WGCW - 3'
BsiHKCI AvaI CYCGRG C^YCGRG
GYGCR^C		 5' - YGCR
BsiKI BstEII GGTNACC G^GTNACC
CCANTG^G		 5' - CANTG
BsiLI EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
BsiMI BspMII TCCGGA T^CCGGA
AGGCC^T		 5' - GGCC
BsiQI BclI TGATCA T^GATCA
ACTAG^T		 5' - CTAG
BsiSI HpaII CCGG C^CGG
GGC^C		 5' - GC
BsiWI SplI CGTACG C^GTACG
GCATG^C		 5' - CATG
BsiXI ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
BsiYI CCNNNNNNNGG CCNNNNN^NNGG
GGNN^NNNNNCC		 NNN - 3'
BsiZI AsuI GGNCC G^GNCC
CCNG^G		 5' - CNG
BslI BsiYI CCNNNNNNNGG CCNNNNN^NNGG
GGNN^NNNNNCC		 NNN - 3'
BslFI FinI GGGAC GGGACNNNNNNNNNN^NNNN
CCCTGNNNNNNNNNNNNNN^		 5' - NNNN
BsmI GAATGC GAATGCN^
CTTAC^GN		 CN - 3'
BsmAI GTCTC GTCTCN^NNNN
CAGAGNNNNN^		 5' - NNNN
BsmBI Esp3I CGTCTC CGTCTCN^NNNN
GCAGAGNNNNN^		 5' - NNNN
BsmFI FinI GGGAC GGGACNNNNNNNNNN^NNNN
CCCTGNNNNNNNNNNNNNN^		 5' - NNNN
BsmSI StyI CCWWGG C^CWWGG
GGWWC^C		 5' - GWWC
Bso31I Eco31I GGTCTC GGTCTCN^NNNN
CCAGAGNNNNN^		 5' - NNNN
BsoBI AvaI CYCGRG C^YCGRG
GYGCR^C		 5' - YGCR
BsoCI SduI GDGCHC GDGCH^C
C^DCGHG		 DGCH - 3'
BsoFI Fnu4HI GCNGC GC^NGC
CGN^CG		 5' - N
BsoMAI BsmAI GTCTC GTCTCN^NNNN
CAGAGNNNNN^		 5' - NNNN
Bsp6I Fnu4HI GCNGC GC^NGC
CGN^CG		 5' - N
Bsp13I BspMII TCCGGA T^CCGGA
AGGCC^T		 5' - GGCC
Bsp19I NcoI CCATGG C^CATGG
GGTAC^C		 5' - GTAC
Bsp24I GACNNNNNNTGG GACNNNNNNTGGNNNNNNNNNNNN^
CTGNNNNNNACCNNNNNNN^NNNNN		 NNNNN - 3'
Bsp50I FnuDII CGCG CG^CG
GC^GC		 Blunt
Bsp63I PstI CTGCAG CTGCA^G
G^ACGTC		 TGCA - 3'
Bsp67I MboI GATC ^GATC
CTAG^		 5' - CTAG
Bsp68I NruI TCGCGA TCG^CGA
AGC^GCT		 Blunt
Bsp98I BamHI GGATCC G^GATCC
CCTAG^G		 5' - CTAG
Bsp105I MboI GATC ^GATC
CTAG^		 5' - CTAG
Bsp106I ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
Bsp119I AsuII TTCGAA TT^CGAA
AAGC^TT		 5' - GC
Bsp120I ApaI GGGCCC G^GGCCC
CCCGG^G		 5' - CCGG
Bsp123I FnuDII CGCG CG^CG
GC^GC		 Blunt
Bsp143I MboI GATC ^GATC
CTAG^		 5' - CTAG
Bsp143II HaeII RGCGCY RGCGC^Y
R^CGCGY		 GCGC - 3'
Bsp211I HaeIII GGCC GG^CC
CC^GG		 Blunt
Bsp423I BbvI GCAGC GCAGCNNNNNNNN^NNNN
CGTCGNNNNNNNNNNNN^		 5' - NNNN
Bsp519I HgiJII GRGCYC GRGCY^C
C^RCGYG		 RGCY - 3'
Bsp1286I SduI GDGCHC GDGCH^C
C^DCGHG		 DGCH - 3'
Bsp1407I TGTACA T^GTACA
ACATG^T		 5' - CATG
Bsp1720I EspI GCTNAGC GC^TNAGC
CGANT^CG		 5' - ANT
Bsp1894I AsuI GGNCC G^GNCC
CCNG^G		 5' - CNG
Bsp2095I MboI GATC ^GATC
CTAG^		 5' - CTAG
Bsp4009I BamHI GGATCC G^GATCC
CCTAG^G		 5' - CTAG
BspAI MboI GATC ^GATC
CTAG^		 5' - CTAG
BspA2I AvrII CCTAGG C^CTAGG
GGATC^C		 5' - GATC
Bsp153AI PvuII CAGCTG CAG^CTG
GTC^GAC		 Blunt
BspAAI XhoI CTCGAG C^TCGAG
GAGCT^C		 5' - AGCT
BspAAII XbaI TCTAGA T^CTAGA
AGATC^T		 5' - GATC
BspAAIII BamHI GGATCC G^GATCC
CCTAG^G		 5' - CTAG
BspANI HaeIII GGCC GG^CC
CC^GG		 Blunt
BspBI PstI CTGCAG CTGCA^G
G^ACGTC		 TGCA - 3'
BspBII AsuI GGNCC G^GNCC
CCNG^G		 5' - CNG
BspBRI HaeIII GGCC GG^CC
CC^GG		 Blunt
BspBS31I BbvII GAAGAC GAAGACNN^NNNN
CTTCTGNNNNNN^		 5' - NNNN
BspCI PvuI CGATCG CGAT^CG
GC^TAGC		 AT - 3'
BspCNI BseMII CTCAG CTCAGNNNNNNNNN^
GAGTCNNNNNNN^NN		 NN - 3'
BspDI ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
BspEI BspMII TCCGGA T^CCGGA
AGGCC^T		 5' - GGCC
BspFI MboI GATC ^GATC
CTAG^		 5' - CTAG
BspF4I AsuI GGNCC G^GNCC
CCNG^G		 5' - CNG
BspHI TCATGA T^CATGA
AGTAC^T		 5' - GTAC
BspIS4I BbvII GAAGAC GAAGACNN^NNNN
CTTCTGNNNNNN^		 5' - NNNN
BspJI MboI GATC ^GATC
CTAG^		 5' - CTAG
BspJII ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
BspKI HaeIII GGCC GG^CC
CC^GG		 Blunt
BspKT5I Eco57I CTGAAG CTGAAGNNNNNNNNNNNNNNNN^
GACTTCNNNNNNNNNNNNNN^NN		 NN - 3'
BspKT6I MboI GATC GAT^C
C^TAG		 AT - 3'
BspKT8I HindIII AAGCTT A^AGCTT
TTCGA^A		 5' - TCGA
BspLI NlaIV GGNNCC GGN^NCC
CCN^NGG		 Blunt
BspLAI HhaI GCGC GCG^C
C^GCG		 CG - 3'
BspLAII AsuII TTCGAA TT^CGAA
AAGC^TT		 5' - GC
BspLAIII HindIII AAGCTT A^AGCTT
TTCGA^A		 5' - TCGA
BspLS2I SduI GDGCHC GDGCH^C
C^DCGHG		 DGCH - 3'
BspLU4I AvaI CYCGRG C^YCGRG
GYGCR^C		 5' - YGCR
BspLU11I ACATGT A^CATGT
TGTAC^A		 5' - GTAC
BspLU11III FinI GGGAC GGGACNNNNNNNNNN^NNNN
CCCTGNNNNNNNNNNNNNN^		 5' - NNNN
BspMI ACCTGC ACCTGCNNNN^NNNN
TGGACGNNNNNNNN^		 5' - NNNN
BspMII TCCGGA T^CCGGA
AGGCC^T		 5' - GGCC
BspM39I PvuII CAGCTG CAG^CTG
GTC^GAC		 Blunt
BspM90I SnaI GTATAC GTA^TAC
CAT^ATG		 Blunt
BspMAI PstI CTGCAG CTGCA^G
G^ACGTC		 TGCA - 3'
BspMKI SalI GTCGAC G^TCGAC
CAGCT^G		 5' - AGCT
BspNI EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
BspO4I PvuII CAGCTG CAG^CTG
GTC^GAC		 Blunt
BspOVI Eam1105I GACNNNNNGTC GACNNN^NNGTC
CTGNN^NNNCAG		 N - 3'
BspOVII ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
BspPI BinI GGATC GGATCNNNN^N
CCTAGNNNNN^		 5' - N
BspRI HaeIII GGCC GG^CC
CC^GG		 Blunt
BspR7I SauI CCTNAGG CC^TNAGG
GGANT^CC		 5' - ANT
BspST5I SfaNI GCATC GCATCNNNNN^NNNN
CGTAGNNNNNNNNN^		 5' - NNNN
BspTI AflII CTTAAG C^TTAAG
GAATT^C		 5' - AATT
BspT104I AsuII TTCGAA TT^CGAA
AAGC^TT		 5' - GC
BspT107I HgiCI GGYRCC G^GYRCC
CCYRG^G		 5' - CYRG
BspTNI Eco31I GGTCTC GGTCTCN^NNNN
CCAGAGNNNNN^		 5' - NNNN
BspTS514I BbvII GAAGAC GAAGACNN^NNNN
CTTCTGNNNNNN^		 5' - NNNN
BspWI MwoI GCNNNNNNNGC GCNNNNN^NNGC
CGNN^NNNNNCG		 NNN - 3'
BspXI ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
BspXII BclI TGATCA T^GATCA
ACTAG^T		 5' - CTAG
BspZEI ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
BsrI ACTGG ACTGGN^
TGAC^CN		 GN - 3'
BsrAI AvaII GGWCC G^GWCC
CCWG^G		 5' - CWG
BsrBI CCGCTC CCG^CTC
GGC^GAG		 Blunt
BsrBRI BsaBI GATNNNNATC GATNN^NNATC
CTANN^NNTAG		 Blunt
BsrDI GCAATG GCAATGNN^
CGTTAC^NN		 NN - 3'
BsrFI Cfr10I RCCGGY R^CCGGY
RGGCC^Y		 5' - GGCC
BsrGI Bsp1407I TGTACA T^GTACA
ACATG^T		 5' - CATG
BsrSI BsrI ACTGG ACTGGN^
TGAC^CN		 GN - 3'
BssAI Cfr10I RCCGGY R^CCGGY
RGGCC^Y		 5' - GGCC
BssECI SecI CCNNGG C^CNNGG
GGNNC^C		 5' - GNNC
BssHI XhoI CTCGAG C^TCGAG
GAGCT^C		 5' - AGCT
BssHII BsePI GCGCGC G^CGCGC
CGCGC^G		 5' - GCGC
BssIMI SimI GGGTC GG^GTC
CCCAG^		 5' - CAG
BssKI ScrFI CCNGG ^CCNGG
GGNCC^		 5' - GGNCC
BssNAI SnaI GTATAC GTA^TAC
CAT^ATG		 Blunt
BssSI BsiI CACGAG C^ACGAG
GTGCT^C		 5' - TGCT
BssT1I StyI CCWWGG C^CWWGG
GGWWC^C		 5' - GWWC
BstI BamHI GGATCC G^GATCC
CCTAG^G		 5' - CTAG
Bst1I EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
Bst2I EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
Bst6I Ksp632I CTCTTC CTCTTCN^NNN
GAGAAGNNNN^		 5' - NNN
Bst11I BsrI ACTGG ACTGGN^
TGAC^CN		 GN - 3'
Bst12I BbvI GCAGC GCAGCNNNNNNNN^NNNN
CGTCGNNNNNNNNNNNN^		 5' - NNNN
Bst19I SfaNI GCATC GCATCNNNN^NN
CGTAGNNNNNN^		 5' - NN
Bst19II MboI GATC ^GATC
CTAG^		 5' - CTAG
Bst28I ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
Bst38I EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
Bst40I HpaII CCGG C^CGG
GGC^C		 5' - GC
Bst71I BbvI GCAGC GCAGCNNNNNNNN^NNNN
CGTCGNNNNNNNNNNNN^		 5' - NNNN
Bst98I AflII CTTAAG C^TTAAG
GAATT^C		 5' - AATT
Bst100I EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
Bst1107I SnaI GTATAC GTA^TAC
CAT^ATG		 Blunt
BstACI AcyI GRCGYC GR^CGYC
CRGC^YG		 5' - GC
BstAPI ApaBI GCANNNNNTGC GCANNNN^NTGC
CGTN^NNNNACG		 NNN - 3'
BstAUI Bsp1407I TGTACA T^GTACA
ACATG^T		 5' - CATG
BstBI AsuII TTCGAA TT^CGAA
AAGC^TT		 5' - GC
Bst2BI BsiI CACGAG C^ACGAG
GTGCT^C		 5' - TGCT
BstBAI BsaAI YACGTR YAC^GTR
YTG^CAR		 Blunt
BstBSI SnaI GTATAC GTA^TAC
CAT^ATG		 Blunt
BstB7SI Cfr10I RCCGGY R^CCGGY
RGGCC^Y		 5' - GGCC
BstBS32I BbvII GAAGAC GAAGACNN^NNNN
CTTCTGNNNNNN^		 5' - NNNN
BstBZ153I BsePI GCGCGC G^CGCGC
CGCGC^G		 5' - GCGC
Bst4CI Tsp4CI ACNGT ACN^GT
TG^NCA		 N - 3'
BstC8I Cac8I GCNNGC GCN^NGC
CGN^NCG		 Blunt
BstD102I BsrBI CCGCTC CCG^CTC
GGC^GAG		 Blunt
BstDEI DdeI CTNAG C^TNAG
GANT^C		 5' - ANT
BstDSI DsaI CCRYGG C^CRYGG
GGRYC^C		 5' - GRYC
BstEII GGTNACC G^GTNACC
CCANTG^G		 5' - CANTG
BstENI EcoNI CCTNNNNNAGG CCTNN^NNNAGG
GGANNN^NNTCC		 5' - N
BstENII MboI GATC ^GATC
CTAG^		 5' - CTAG
BstEZ359I HpaI GTTAAC GTT^AAC
CAA^TTG		 Blunt
BstFI HindIII AAGCTT A^AGCTT
TTCGA^A		 5' - TCGA
BstF5I FokI GGATG GGATGNN^
CCTAC^NN		 NN - 3'
BstFNI FnuDII CGCG CG^CG
GC^GC		 Blunt
BstFZ438I FauI CCCGC CCCGCNNNN^NN
GGGCGNNNNNN^		 5' - NN
BstGZ53I Esp3I CGTCTC CGTCTCN^NNNN
GCAGAGNNNNN^		 5' - NNNN
BstH2I HaeII RGCGCY RGCGC^Y
R^CGCGY		 GCGC - 3'
BstH9I BinI GGATC GGATCNNNN^N
CCTAGNNNNN^		 5' - N
BstHHI HhaI GCGC GCG^C
C^GCG		 CG - 3'
BstHPI HpaI GTTAAC GTT^AAC
CAA^TTG		 Blunt
BstHZ55I BstXI CCANNNNNNTGG CCANNNNN^NTGG
GGTN^NNNNNACC		 NNNN - 3'
BstIZ316I DraIII CACNNNGTG CACNNN^GTG
GTG^NNNCAC		 NNN - 3'
BstJZ301I DdeI CTNAG C^TNAG
GANT^C		 5' - ANT
BstKTI MboI GATC GAT^C
C^TAG		 AT - 3'
BstM6I EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
BstMBI MboI GATC ^GATC
CTAG^		 5' - CTAG
BstMCI McrI CGRYCG CGRY^CG
GC^RYGC		 RY - 3'
BstMWI MwoI GCNNNNNNNGC GCNNNNN^NNGC
CGNN^NNNNNCG		 NNN - 3'
BstMZ611I ScrFI CCNGG ^CCNGG
GGNCC^		 5' - GGNCC
BstNI EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
Bst31NI BsrBI CCGCTC CCG^CTC
GGC^GAG		 Blunt
BstNSI NspI RCATGY RCATG^Y
R^GTACY		 CATG - 3'
BstNZ169I ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
BstOI EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
BstOZ616I FinI GGGAC GGGACNNNNNNNNNN^NNNN
CCCTGNNNNNNNNNNNNNN^		 5' - NNNN
BstPI BstEII GGTNACC G^GTNACC
CCANTG^G		 5' - CANTG
BstPAI PshAI GACNNNNGTC GACNN^NNGTC
CTGNN^NNCAG		 Blunt
BstPZ418I FokI GGATG GGATGNNNNNNNNN^NNNN
CCTACNNNNNNNNNNNNN^		 5' - NNNN
BstPZ740I AflII CTTAAG C^TTAAG
GAATT^C		 5' - AATT
BstRZ246I SwaI ATTTAAAT ATTT^AAAT
TAAA^TTTA		 Blunt
BstSI AvaI CYCGRG C^YCGRG
GYGCR^C		 5' - YGCR
BstSCI ScrFI CCNGG ^CCNGG
GGNCC^		 5' - GGNCC
BstSFI SfeI CTRYAG C^TRYAG
GARYT^C		 5' - ARYT
BstSNI SnaBI TACGTA TAC^GTA
ATG^CAT		 Blunt
BstSWI SwaI ATTTAAAT ATTT^AAAT
TAAA^TTTA		 Blunt
BstT7I BclI TGATCA T^GATCA
ACTAG^T		 5' - CTAG
BstT9I BstEII GGTNACC G^GTNACC
CCANTG^G		 5' - CANTG
BstT10I BstEII GGTNACC G^GTNACC
CCANTG^G		 5' - CANTG
Bst31TI BinI GGATC GGATCNNNN^N
CCTAGNNNNN^		 5' - N
BstTS5I BbvII GAAGAC GAAGACNN^NNNN
CTTCTGNNNNNN^		 5' - NNNN
BstUI FnuDII CGCG CG^CG
GC^GC		 Blunt
Bst2UI EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
BstVI XhoI CTCGAG C^TCGAG
GAGCT^C		 5' - AGCT
BstV1I BbvI GCAGC GCAGCNNNNNNNN^NNNN
CGTCGNNNNNNNNNNNN^		 5' - NNNN
BstV2I BbvII GAAGAC GAAGACNN^NNNN
CTTCTGNNNNNN^		 5' - NNNN
BstXI CCANNNNNNTGG CCANNNNN^NTGG
GGTN^NNNNNACC		 NNNN - 3'
BstX2I XhoII RGATCY R^GATCY
RCTAG^Y		 5' - CTAG
BstYI XhoII RGATCY R^GATCY
RCTAG^Y		 5' - CTAG
BstZI XmaIII CGGCCG C^GGCCG
GCCGG^C		 5' - CCGG
BstZ17I SnaI GTATAC GTA^TAC
CAT^ATG		 Blunt
Bsu6I Ksp632I CTCTTC CTCTTCN^NNN
GAGAAGNNNN^		 5' - NNN
Bsu15I ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
Bsu23I BspMII TCCGGA T^CCGGA
AGGCC^T		 5' - GGCC
Bsu36I SauI CCTNAGG CC^TNAGG
GGANT^CC		 5' - ANT
Bsu54I AsuI GGNCC G^GNCC
CCNG^G		 5' - CNG
Bsu1532I FnuDII CGCG CG^CG
GC^GC		 Blunt
Bsu1854I HgiJII GRGCYC GRGCY^C
C^RCGYG		 RGCY - 3'
BsuBI PstI CTGCAG CTGCA^G
G^ACGTC		 TGCA - 3'
BsuFI HpaII CCGG C^CGG
GGC^C		 5' - GC
BsuRI HaeIII GGCC GG^CC
CC^GG		 Blunt
BsuTUI ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
BteI HaeIII GGCC GG^CC
CC^GG		 Blunt
BtgI DsaI CCRYGG C^CRYGG
GGRYC^C		 5' - GRYC
BtgZI GCGATG GCGATGNNNNNNNNNN^NNNN
CGCTACNNNNNNNNNNNNNN^		 5' - NNNN
BthAI AvaII GGWCC G^GWCC
CCWG^G		 5' - CWG
BthCI Fnu4HI GCNGC GCNG^C
C^GNCG		 CNG - 3'
BthDI EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
BthEI EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
BtkI FnuDII CGCG CG^CG
GC^GC		 Blunt
BtkII MboI GATC ^GATC
CTAG^		 5' - CTAG
BtrI CACGTC CAC^GTC
GTG^CAG		 Blunt
BtsI GCAGTG GCAGTGNN^
CGTCAC^NN		 NN - 3'
BveI BspMI ACCTGC ACCTGCNNNN^NNNN
TGGACGNNNNNNNN^		 5' - NNNN
BvuI HgiJII GRGCYC GRGCY^C
C^RCGYG		 RGCY - 3'
BvuBI SplI CGTACG C^GTACG
GCATG^C		 5' - CATG
BaeGI BseSI GKGCMC GKGCM^C
C^KCGMG		 KGCM - 3'
BarI ^
^		 Blunt
BciT130I EcoRII CCWGG CC^WGG
GGW^CC		 5' - W
BcoDI BsmAI GTCTC GTCTCN^NNNN
CAGAGNNNNN^		 5' - NNNN
BdaI ^
^		 Blunt
BisI Fnu4HI GCNGC GC^NGC
CGN^CG		 5' - N
BlsI GCNGC GCN^GC
CG^NCG		 N - 3'
BmcAI ScaI AGTACT AGT^ACT
TCA^TGA		 Blunt
BmeRI AhdI GACNNNNNGTC GACNNN^NNGTC
CTGNN^NNNCAG		 N - 3'
BmeT110I CYCGRG CY^CGRG
GYGC^RC		 5' - GC
BmgT120I GGNCC GG^NCC
CCN^GG		 5' - N
BmiI GGNNCC GGN^NCC
CCN^NGG		 Blunt
BmrFI CCNGG CC^NGG
GGN^CC		 5' - N
BmuI ACTGGG（NNNN�
�N
 ACTGGG（NN^NN）N
TGACCC（N^NNN）N		 N - 3'
BpuMI CauII CCSGG CC^SGG
GGS^CC		 5' - S
BpvUI PvuI CGATCG CGAT^CG
GC^TAGC		 AT - 3'
BshVI ClaI ATCGAT AT^CGAT
TAGC^TA		 5' - GC
BsnI HaeIII GGCC GG^CC
CC^GG		 Blunt
BspACI AciI CCGC C^CGC
GGC^G		 5' - GC
BspFNI FnuDII CGCG CG^CG
GC^GC		 Blunt
BspOI GCTAGC GCTAG^C
C^GATCG 		CTAG - 3'
BspQI GCTCTTCNNNN GCTCTTCN^NNN
CGAGAAGNNNN^		 5' - NNN
BssMI MboI GATC ^GATC
CTAG^		 5' - CTAG
BssNI GRCGYC GR^CGYC
CRGC^YG		 5' - GC
BstAFI CTTAAG C^TTAAG
GAATT^C		 5' - AATT
BstMAI GTCTCNNNNN GTCTCN^NNNN
CAGAGNNNNN^		 5' - NNNN
BstSLI GKGCMC GKGCM^C
C^KCGMG		 KGCM - 3'
BtsCI GGATGNN GGATGNN^
CCTAC^NN		 NN - 3'
BtsIMutI CAGTG CAGTGNN^
GTCAC^NN		 NN - 3'
BtuMI tcgcga tcg^cga
tcg^cga		 Blunt