LOC105342461 cDNA ORF clone, Crassostrea gigas(Pacific oyster)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105342461
Entrez Gene ID	105342461
Full Name	calmodulin-regulated spectrin-associated protein 1
Gene Type	protein-coding
Organism	Crassostrea gigas(Pacific oyster)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011449422.2	XP_011447724.1	calmodulin-regulated spectrin-associated protein 1 isoform X2
XM_011449420.2	XP_011447722.1	calmodulin-regulated spectrin-associated protein 1 isoform X1
XM_011449423.2	XP_011447725.1	calmodulin-regulated spectrin-associated protein 1 isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC105342461 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105342461 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCr109219	XM_011449420.2 Latest version!	Crassostrea gigas calmodulin-regulated spectrin-associated protein 1 (LOC105342461), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCr109220	XM_011449422.2 Latest version!	Crassostrea gigas calmodulin-regulated spectrin-associated protein 1 (LOC105342461), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCr109221	XM_011449423.2 Latest version!	Crassostrea gigas calmodulin-regulated spectrin-associated protein 1 (LOC105342461), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105342461 ( XM_011449420.2 ) cDNA ORF clone, Crassostrea gigas(Pacific oyster) -> XP_011447722.1 Crassostrea gigas calmodulin-regulated spectrin-associated protein 1 (LOC105342461), transcript variant X1, mRNA.

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CloneID	OCr109219
Clone ID Related Accession (Same CDS sequence)	XM_011449420.2
Accession Version	XM_011449420.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-01
Organism	Crassostrea gigas(Pacific oyster)
Product	calmodulin-regulated spectrin-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011936675.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 2, 2017 this sequence version replaced XM_011449420.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crassostrea gigas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAAAAT CATCAAACAT GAATCAGTCT GTGGAAAGTG CCCCTCCTGT TGATGACATC 
ATCGAAATCA TACCCATGGA CGAGTATGAT AGCAACAAGG CAAAACTTCA GTGCTCTGTA 
TCATGGATTC TCGACAAGGC CTACAGAGAT CAAATACCAA AGGAATTCAA AGACCCATTT 
TACGAGACAC AGGATGGTGT GATGCATATT AAGCCCAAGC TGATGAATCT GATGGTTTCC 
AGTGAGCTTT ACTGTCAGGC CTGCAATAAT ATGTTCGGGG ATTCATCATT ACAATGGCAA 
GGGACATCAG GTCATTCCAG CATCATTCAA GTGATGTCCA GAAAAGGGAT ATATGTTGTT 
GATGATAACG ATAAAGCTGT TACAGAGTCT GTACTTATAA AGTCGGCTCC CTTTGATGTG 
AGTGCACACA TTGCCCTGAT CGAAGCCTTG ATGCTGGCCT ACGTTAGACA GATAGTGACG 
GTGGAGAAAG TGGTACAAAC CGTCCGAAGG TTCGCTGCCT TCAATGCCTC CAGTGAGCTG 
CCGTCCAGTG TGGAGGATGC TCTCCTGTTC TGGATAAACA AAGTCTGTGT GGCCGTACAG 
CTCAGTGTGA ACAAGGAGGA TAACCAGGTG CTGGAAGGGG AGACAAATCA GAAGGTTCGT 
ATTGTGAGTA AGTCTGGGAT GAGCATGAGG GAGTCTGTGA ATATTCCACT GATAGAGGAC 
CTCTCTCAGG ACATTGGTGA TGGCTGTTCT CTGGCTGTTC TAATCAGCTT CTACTGCCCC 
TCCGCCCTCA GTCAGCAAGA TATCTGTTTA AAGTCTAATG TAGGCATAGC TGACTCGCTA 
TATAACCTTC GCCTAGTGAA GGAATTCTGT GATAACTACA TCCCTGGCAA AGCCTTCCAT 
CTGACCTACG AAGACTTCCT GTACATGCAC AACAACCTGC GACAGAACGT GGAGGTGTTT 
CTCGCAGAGC TGTTCTACTG GTTTGAGATT CAGAAGCTGG ACTGTGTGTA TAGTGAGCCA 
GGTTCGCGGC CCACAACAGC CAAGAAAATG ACCAGCAGCG CCATCACAAA TCTTCCTCCG 
ATCAGTAACG TCACCAAGCA GAGTTTCCAC AAGTACTCTG CGGATGATGG GGCGGTTCCT 
CGCGGAGTGA GCCAGATGCC ACCAAGACAA CCCCTCCTGT ATAAACGGCA GCAGGAACCT 
GATGTCAGTC AGCAAGGTCC AGGTGTGGGA CGATCAAGAC CAATGTCTGC TGTGGTCGGG 
AAGGAGAAGA ATGATGTACA TCAGTCAGTG CTGGCCTGGC AGGAGAACAG TCAACAGACT 
GTTCAGCAGA GTGGTGGTAG TAACCTCCTG GCCAATGTCA GCATTGACTC GGAGTTGATG 
GAGAGCTTTT CTGATGATCA GCTGGGAAGT CTCGACATGG ATCTGAGTGA CACCCCCCGG 
CCCACTCTGC CAGCCAATCA AGTGCCAAGA GAATCAGCAT CAAATCTACG TGATCCAAAT 
CATCCGGATT ATATGGAGGT ACAAAGTGTG ACATCAGGAG CTGCCACAAA TAGAACTGTT 
CATCAAGGGG AGGAAACTAG ACCTGATAGT GAGTCTGCTA TACCTGAGAA TCCTCCTTGT 
CATCAAGACT TGCTAAATGA ATTCAGTGCT CAATTAGACC CTAAAGACAA TAAGCAGAGT 
AGAAATGAAT GCTATGAGCC TTTACTACCT GCAAAATTAA AACCTCGGAA AGAAGCAATA 
AATAACCACA GCAAGGAACA AGAGAGGGGA GATAAGTCTT TACTTGCTAA ATCAGTGAAA 
TCTATGGAAG TGGATCTGGG TTCTTCAAGT GTTTTAAGTG AGCGAAGTGA CAGCAGTAGT 
TCTGTTCCCG TCACTCCTCT ACCCGAGGAT CAGTTATCGG ACCAAGATGT TCCCAAACAG 
CCCCGTTATA CAGCATTTAC AGTCAACACA GGCTTTTCAG AAGACATGGA ATCTCCACGA 
AAAAGTGTAC TGTCCACCAT CCGTAGCCAA GGCGATACTG CTGAATCAGT TAAACTGAGT 
TATACTGTCT CCAATGAGGC CACTTCAGAA TCGGCGCGGG CAGCCGGGAT ACCTGTTGTC 
GGGGAGAGTG GCGAACAAGC ATGGAGGAGA TGGGGCTCCA GAGAGGGTAG TGTGAACAGT 
AGGAGTTCTG GGGACAATTC CGATCATGAA TCTCAGAAAA TTCACTACGA CCAGAAACTG 
AGTGAGAACA AAGACAAAAT CCGAGAGGTG TCACCTCCAA AAACTTTTAC TAGATTCGGT 
TCCAAGGACA GTGATGTATT TCAAAAACCG AAAATGATAG TTAGAAGTAA GACAGCTGTT 
TCACCAAAGA AAGAACCCTC CAAACATATG ACAACAAATT TTGCTGAAAT AAAGAGAATG 
AAGAATGTTC AAGGGAATGT TGACAATTCT GGAATGGTTT ACATGCAGCA TGGCCATGAA 
GTGAATAACA AAGCTATGCA AAGCTCAACA ACTCAAATCA ACAATAGGAA CAACCAAAAC 
TCAACGTCAC CTAGCAGAAC AACATGGCAG CAAGCAAGCA CTCTAAAATC TCCTGATCAG 
GCCACAAGTG ATTCCTCAGA AAGCAGTGAC AGTAGTCGCC CCGTGCTGTC TGAGCTAACA 
GAAATCCGGC TTAAGTTGGA GGAAAAGCGT AAACAGATAG AGAAAAAGAA GAACAGAATG 
GAGCTACAGC AACAGAAACA GAGACAGCGG CTAGGGAAGA CAGCTTTTCT TCATGTGCTG 
ACAAAACCCA CTGAGGAGGA GGTGTCTGAT GACTTGAGTT CACAGGGAAG TGAGGCGTCT 
CTACCAGCAA GTGAGGAGGC TGCCTCAAGT TCAACCTCGG ACAAAGGACC AACAGCAGCA 
GATGGAAAAA CTACAGAAGC ACCTCAAAGC AACAAAGCAT TTTCTAGGGA GGGGATACAG 
CAAACAATAG AAAATGTTAG AAAGAAGTGG TTTCAAGAGG ACAAGGATGA GCAACCTGTG 
CAGATGGAGA TAAAACCAGA AGAAATAATG AGTAAGAGTT GTCCAGCCAA AAATGGAGAA 
GTTTTACAAA AACAAAAATC GGCGCTGCCT GCCCAGGAGC AAAATTCAAT GTCAATGATA 
GAAAATATAT CTGACAGAAA ATTATCTGAA GCTCAAGAAT TGGACAGTCC ATCTGTATAC 
AGTCGGAAGC TTGAAAAACT GGATCATGAC CTGACTGATT TGAAAGGGGA GATCCTGAGG 
ATGTCTCTGG AACAGGAGCA GTACAATGGG AGTCCAGCGG CTACTTCCAT GACACACCCA 
TCCACAGCCA CCAGGCCAAC TTATGCAGTG GAAACTCCAG AACGGAAATT CCAGGGAAAA 
CCATTCCATG GATCAGATCT CGCCTCAGTG AATCAGGCAA ACGTAAACAC TCCAGAGAGA 
ATGCAGTATG GAACCCCGGC GGTTTGTCGC CATGCAGGAT ATGCAGGCCC CATTGATCCC 
AGTGTACCCA CCCCAGAGAG GGTTGTGATG GGGGGAGGTA TGTCTCAGTA CCCCCAGGGC 
CTACCCCCAC AGATGCCTAT TACACAACCA TTCCAGCCCC AAGTGATGCC CCACTCTTTA 
CCCTATAATG GACAGAGTCA GTTCAGTCCA ATGCCACAAA CACAGGTGCC ATACAGCGGT 
TTCCAAACTG TTGGTACTAC TCCCTATCCA CAACCCCAGT TCCCTCCCAC CCCACCCCAT 
GGCTACCAAC AGTCCCCTCC CTTCAATCCC AATCTGGTGT CACCCCACGG TCAATATGGG 
ATGCCTGGGG TATATAATAT GACTCCTCAG CAGTACCCTC CCCATTCTCA CAACACATCT 
ACAGTGTCAG CTCATACTCC TGTCTCTTAC ATGTACTCGC CCAATCACAC CGGAGCTCAA 
GTCTTTCAAC CTTCTGCGGA TATTAATAAA ATGCCGCCTG CTTCTCGGGC TGAAATGCCC 
CCCACTTCTA GTGCTTTCAA TGATGGTAAC ATCACCAAAG AAGACACCTC TGCTGTTTCA 
AATATGGGTG TTCATGACTT GTCTCATAAC AAAAGTGAAA TACAAAGTGA CATTTCTAGT 
GACCAGAATG GATTTTTTGT GTCATTTGGA GACAATTCTC CAAAGGTTAA ACCCAAACTT 
TCTAGTGCAA CATCTCAAAA GACGGTAACC TCTGAACCAA CTAGTCTCCA AAACCAAACA 
CAACAGTCAC CTCAGCAGAA CCAGTCTAGT CAAGCCAGCA ACCTGAATGT GTTGGCCGTA 
GATCAGAACA CGTCGGCAAG CTCCCAGCCC GACAACACAT CCCTGCTTGA TACCTCGGGG 
GTCGGGTTTG TGGTGGGAGA AGACCCAGAA ACTATCTCAA AGTCAGTGGA AGAAGAATTG 
AACAGAAAAA AAGAAAAGTT TATACAAATG CAGATCAAAC GCAAAGAAGA ACAAGACAAA 
AAACGTCAAT ATAAAGAGTT GGAAATGTCA CGCAGGAAAG AGCAAGAGAG AATTAAACAA 
GAAGAGGCAG AAAAACGTAA AGCCAGTGAA AAGGCACGAA GAGATGAAAT ATTCCGTCAG 
TACCAGGATA AGAAGCATCA AGAGGAAGAC AACTACGCAC CTCCTGTCCG CAGGAACCGA 
TCCAAAACCC GGGAAAAGAC CCGCCCCAAA TCCATGTTCG ACAAAGGAGA ATCAGGATCA 
GTGGAAGGTT TGCACTCCCA TAGTAGTTCT CAAGAGGATC TCTCTTCTCA AGCTTTTACT 
ATCAGTCCGG GTCACAAAGA ACCTTCGGGT GTTGGTCCTA GAAAACCCCC AAGCTTAGCA 
CCACGCCCAG GAAACATTCG AAAAGCAGTG TCCTGTAACA CCCTGTCCCA ATCCACGGAG 
GGCAAGCTGA CCTACAGGAG ACAGCCTTCT CCAGATCTTG GTCGTTTGAA GAGAGGCAGG 
AACAGTGCAG AGAGCAGTGA ATCTGGATCA GATTATGCAG GACCCAAGTT GTTTGTGAAG 
CCAAGCTCCA AGTCAAACCG CCACATCATC ATCAACGCCA TTAGTCAGTG TGTGCTAGCT 
GGACCCGTGA ACTCCGATCA GAAAAACAAA GTATTGGAGG TTTTGGCTAA ATCAGATGCC 
AAGCACTTCC TGATATTGTT CCGAGACCAC TCCTGTCAGT TCCGGGGACT GTACAGCTTT 
GATCCAGATT CAGAGGACTC CATTAAAATC CATGGAATAG GACCCAAACA AATCACCAGC 
AAAATGATCG AAAGATTTTA CAAGTACAAT TCTGGTGGAA AAAGCTTTAG TGAGGTTACA 
TCTACGAAAC ATTTATCAGT GTCAATAGAC GCAGTAGTTA TACACAATGC CTTGTGGAAA 
ACATCAGCAA AGCCGCCACA AAAAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011447722.1
CDS	229..5598
Translation	MIKSSNMNQSVESAPPVDDIIEIIPMDEYDSNKAKLQCSVSWILDKAYRDQIPKEFKDPFYETQDGVMHIKPKLMNLMVSSELYCQACNNMFGDSSLQWQGTSGHSSIIQVMSRKGIYVVDDNDKAVTESVLIKSAPFDVSAHIALIEALMLAYVRQIVTVEKVVQTVRRFAAFNASSELPSSVEDALLFWINKVCVAVQLSVNKEDNQVLEGETNQKVRIVSKSGMSMRESVNIPLIEDLSQDIGDGCSLAVLISFYCPSALSQQDICLKSNVGIADSLYNLRLVKEFCDNYIPGKAFHLTYEDFLYMHNNLRQNVEVFLAELFYWFEIQKLDCVYSEPGSRPTTAKKMTSSAITNLPPISNVTKQSFHKYSADDGAVPRGVSQMPPRQPLLYKRQQEPDVSQQGPGVGRSRPMSAVVGKEKNDVHQSVLAWQENSQQTVQQSGGSNLLANVSIDSELMESFSDDQLGSLDMDLSDTPRPTLPANQVPRESASNLRDPNHPDYMEVQSVTSGAATNRTVHQGEETRPDSESAIPENPPCHQDLLNEFSAQLDPKDNKQSRNECYEPLLPAKLKPRKEAINNHSKEQERGDKSLLAKSVKSMEVDLGSSSVLSERSDSSSSVPVTPLPEDQLSDQDVPKQPRYTAFTVNTGFSEDMESPRKSVLSTIRSQGDTAESVKLSYTVSNEATSESARAAGIPVVGESGEQAWRRWGSREGSVNSRSSGDNSDHESQKIHYDQKLSENKDKIREVSPPKTFTRFGSKDSDVFQKPKMIVRSKTAVSPKKEPSKHMTTNFAEIKRMKNVQGNVDNSGMVYMQHGHEVNNKAMQSSTTQINNRNNQNSTSPSRTTWQQASTLKSPDQATSDSSESSDSSRPVLSELTEIRLKLEEKRKQIEKKKNRMELQQQKQRQRLGKTAFLHVLTKPTEEEVSDDLSSQGSEASLPASEEAASSSTSDKGPTAADGKTTEAPQSNKAFSREGIQQTIENVRKKWFQEDKDEQPVQMEIKPEEIMSKSCPAKNGEVLQKQKSALPAQEQNSMSMIENISDRKLSEAQELDSPSVYSRKLEKLDHDLTDLKGEILRMSLEQEQYNGSPAATSMTHPSTATRPTYAVETPERKFQGKPFHGSDLASVNQANVNTPERMQYGTPAVCRHAGYAGPIDPSVPTPERVVMGGGMSQYPQGLPPQMPITQPFQPQVMPHSLPYNGQSQFSPMPQTQVPYSGFQTVGTTPYPQPQFPPTPPHGYQQSPPFNPNLVSPHGQYGMPGVYNMTPQQYPPHSHNTSTVSAHTPVSYMYSPNHTGAQVFQPSADINKMPPASRAEMPPTSSAFNDGNITKEDTSAVSNMGVHDLSHNKSEIQSDISSDQNGFFVSFGDNSPKVKPKLSSATSQKTVTSEPTSLQNQTQQSPQQNQSSQASNLNVLAVDQNTSASSQPDNTSLLDTSGVGFVVGEDPETISKSVEEELNRKKEKFIQMQIKRKEEQDKKRQYKELEMSRRKEQERIKQEEAEKRKASEKARRDEIFRQYQDKKHQEEDNYAPPVRRNRSKTREKTRPKSMFDKGESGSVEGLHSHSSSQEDLSSQAFTISPGHKEPSGVGPRKPPSLAPRPGNIRKAVSCNTLSQSTEGKLTYRRQPSPDLGRLKRGRNSAESSESGSDYAGPKLFVKPSSKSNRHIIINAISQCVLAGPVNSDQKNKVLEVLAKSDAKHFLILFRDHSCQFRGLYSFDPDSEDSIKIHGIGPKQITSKMIERFYKYNSGGKSFSEVTSTKHLSVSIDAVVIHNALWKTSAKPPQKR

Target ORF information:

RefSeq Version	XM_011449420.2
Organism	Crassostrea gigas(Pacific oyster)
Definition	Crassostrea gigas calmodulin-regulated spectrin-associated protein 1 (LOC105342461), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011449420.2

ORF Insert Sequence:

ATGATAAAAT CATCAAACAT GAATCAGTCT GTGGAAAGTG CCCCTCCTGT TGATGACATC 
ATCGAAATCA TACCCATGGA CGAGTATGAT AGCAACAAGG CAAAACTTCA GTGCTCTGTA 
TCATGGATTC TCGACAAGGC CTACAGAGAT CAAATACCAA AGGAATTCAA AGACCCATTT 
TACGAGACAC AGGATGGTGT GATGCATATT AAGCCCAAGC TGATGAATCT GATGGTTTCC 
AGTGAGCTTT ACTGTCAGGC CTGCAATAAT ATGTTCGGGG ATTCATCATT ACAATGGCAA 
GGGACATCAG GTCATTCCAG CATCATTCAA GTGATGTCCA GAAAAGGGAT ATATGTTGTT 
GATGATAACG ATAAAGCTGT TACAGAGTCT GTACTTATAA AGTCGGCTCC CTTTGATGTG 
AGTGCACACA TTGCCCTGAT CGAAGCCTTG ATGCTGGCCT ACGTTAGACA GATAGTGACG 
GTGGAGAAAG TGGTACAAAC CGTCCGAAGG TTCGCTGCCT TCAATGCCTC CAGTGAGCTG 
CCGTCCAGTG TGGAGGATGC TCTCCTGTTC TGGATAAACA AAGTCTGTGT GGCCGTACAG 
CTCAGTGTGA ACAAGGAGGA TAACCAGGTG CTGGAAGGGG AGACAAATCA GAAGGTTCGT 
ATTGTGAGTA AGTCTGGGAT GAGCATGAGG GAGTCTGTGA ATATTCCACT GATAGAGGAC 
CTCTCTCAGG ACATTGGTGA TGGCTGTTCT CTGGCTGTTC TAATCAGCTT CTACTGCCCC 
TCCGCCCTCA GTCAGCAAGA TATCTGTTTA AAGTCTAATG TAGGCATAGC TGACTCGCTA 
TATAACCTTC GCCTAGTGAA GGAATTCTGT GATAACTACA TCCCTGGCAA AGCCTTCCAT 
CTGACCTACG AAGACTTCCT GTACATGCAC AACAACCTGC GACAGAACGT GGAGGTGTTT 
CTCGCAGAGC TGTTCTACTG GTTTGAGATT CAGAAGCTGG ACTGTGTGTA TAGTGAGCCA 
GGTTCGCGGC CCACAACAGC CAAGAAAATG ACCAGCAGCG CCATCACAAA TCTTCCTCCG 
ATCAGTAACG TCACCAAGCA GAGTTTCCAC AAGTACTCTG CGGATGATGG GGCGGTTCCT 
CGCGGAGTGA GCCAGATGCC ACCAAGACAA CCCCTCCTGT ATAAACGGCA GCAGGAACCT 
GATGTCAGTC AGCAAGGTCC AGGTGTGGGA CGATCAAGAC CAATGTCTGC TGTGGTCGGG 
AAGGAGAAGA ATGATGTACA TCAGTCAGTG CTGGCCTGGC AGGAGAACAG TCAACAGACT 
GTTCAGCAGA GTGGTGGTAG TAACCTCCTG GCCAATGTCA GCATTGACTC GGAGTTGATG 
GAGAGCTTTT CTGATGATCA GCTGGGAAGT CTCGACATGG ATCTGAGTGA CACCCCCCGG 
CCCACTCTGC CAGCCAATCA AGTGCCAAGA GAATCAGCAT CAAATCTACG TGATCCAAAT 
CATCCGGATT ATATGGAGGT ACAAAGTGTG ACATCAGGAG CTGCCACAAA TAGAACTGTT 
CATCAAGGGG AGGAAACTAG ACCTGATAGT GAGTCTGCTA TACCTGAGAA TCCTCCTTGT 
CATCAAGACT TGCTAAATGA ATTCAGTGCT CAATTAGACC CTAAAGACAA TAAGCAGAGT 
AGAAATGAAT GCTATGAGCC TTTACTACCT GCAAAATTAA AACCTCGGAA AGAAGCAATA 
AATAACCACA GCAAGGAACA AGAGAGGGGA GATAAGTCTT TACTTGCTAA ATCAGTGAAA 
TCTATGGAAG TGGATCTGGG TTCTTCAAGT GTTTTAAGTG AGCGAAGTGA CAGCAGTAGT 
TCTGTTCCCG TCACTCCTCT ACCCGAGGAT CAGTTATCGG ACCAAGATGT TCCCAAACAG 
CCCCGTTATA CAGCATTTAC AGTCAACACA GGCTTTTCAG AAGACATGGA ATCTCCACGA 
AAAAGTGTAC TGTCCACCAT CCGTAGCCAA GGCGATACTG CTGAATCAGT TAAACTGAGT 
TATACTGTCT CCAATGAGGC CACTTCAGAA TCGGCGCGGG CAGCCGGGAT ACCTGTTGTC 
GGGGAGAGTG GCGAACAAGC ATGGAGGAGA TGGGGCTCCA GAGAGGGTAG TGTGAACAGT 
AGGAGTTCTG GGGACAATTC CGATCATGAA TCTCAGAAAA TTCACTACGA CCAGAAACTG 
AGTGAGAACA AAGACAAAAT CCGAGAGGTG TCACCTCCAA AAACTTTTAC TAGATTCGGT 
TCCAAGGACA GTGATGTATT TCAAAAACCG AAAATGATAG TTAGAAGTAA GACAGCTGTT 
TCACCAAAGA AAGAACCCTC CAAACATATG ACAACAAATT TTGCTGAAAT AAAGAGAATG 
AAGAATGTTC AAGGGAATGT TGACAATTCT GGAATGGTTT ACATGCAGCA TGGCCATGAA 
GTGAATAACA AAGCTATGCA AAGCTCAACA ACTCAAATCA ACAATAGGAA CAACCAAAAC 
TCAACGTCAC CTAGCAGAAC AACATGGCAG CAAGCAAGCA CTCTAAAATC TCCTGATCAG 
GCCACAAGTG ATTCCTCAGA AAGCAGTGAC AGTAGTCGCC CCGTGCTGTC TGAGCTAACA 
GAAATCCGGC TTAAGTTGGA GGAAAAGCGT AAACAGATAG AGAAAAAGAA GAACAGAATG 
GAGCTACAGC AACAGAAACA GAGACAGCGG CTAGGGAAGA CAGCTTTTCT TCATGTGCTG 
ACAAAACCCA CTGAGGAGGA GGTGTCTGAT GACTTGAGTT CACAGGGAAG TGAGGCGTCT 
CTACCAGCAA GTGAGGAGGC TGCCTCAAGT TCAACCTCGG ACAAAGGACC AACAGCAGCA 
GATGGAAAAA CTACAGAAGC ACCTCAAAGC AACAAAGCAT TTTCTAGGGA GGGGATACAG 
CAAACAATAG AAAATGTTAG AAAGAAGTGG TTTCAAGAGG ACAAGGATGA GCAACCTGTG 
CAGATGGAGA TAAAACCAGA AGAAATAATG AGTAAGAGTT GTCCAGCCAA AAATGGAGAA 
GTTTTACAAA AACAAAAATC GGCGCTGCCT GCCCAGGAGC AAAATTCAAT GTCAATGATA 
GAAAATATAT CTGACAGAAA ATTATCTGAA GCTCAAGAAT TGGACAGTCC ATCTGTATAC 
AGTCGGAAGC TTGAAAAACT GGATCATGAC CTGACTGATT TGAAAGGGGA GATCCTGAGG 
ATGTCTCTGG AACAGGAGCA GTACAATGGG AGTCCAGCGG CTACTTCCAT GACACACCCA 
TCCACAGCCA CCAGGCCAAC TTATGCAGTG GAAACTCCAG AACGGAAATT CCAGGGAAAA 
CCATTCCATG GATCAGATCT CGCCTCAGTG AATCAGGCAA ACGTAAACAC TCCAGAGAGA 
ATGCAGTATG GAACCCCGGC GGTTTGTCGC CATGCAGGAT ATGCAGGCCC CATTGATCCC 
AGTGTACCCA CCCCAGAGAG GGTTGTGATG GGGGGAGGTA TGTCTCAGTA CCCCCAGGGC 
CTACCCCCAC AGATGCCTAT TACACAACCA TTCCAGCCCC AAGTGATGCC CCACTCTTTA 
CCCTATAATG GACAGAGTCA GTTCAGTCCA ATGCCACAAA CACAGGTGCC ATACAGCGGT 
TTCCAAACTG TTGGTACTAC TCCCTATCCA CAACCCCAGT TCCCTCCCAC CCCACCCCAT 
GGCTACCAAC AGTCCCCTCC CTTCAATCCC AATCTGGTGT CACCCCACGG TCAATATGGG 
ATGCCTGGGG TATATAATAT GACTCCTCAG CAGTACCCTC CCCATTCTCA CAACACATCT 
ACAGTGTCAG CTCATACTCC TGTCTCTTAC ATGTACTCGC CCAATCACAC CGGAGCTCAA 
GTCTTTCAAC CTTCTGCGGA TATTAATAAA ATGCCGCCTG CTTCTCGGGC TGAAATGCCC 
CCCACTTCTA GTGCTTTCAA TGATGGTAAC ATCACCAAAG AAGACACCTC TGCTGTTTCA 
AATATGGGTG TTCATGACTT GTCTCATAAC AAAAGTGAAA TACAAAGTGA CATTTCTAGT 
GACCAGAATG GATTTTTTGT GTCATTTGGA GACAATTCTC CAAAGGTTAA ACCCAAACTT 
TCTAGTGCAA CATCTCAAAA GACGGTAACC TCTGAACCAA CTAGTCTCCA AAACCAAACA 
CAACAGTCAC CTCAGCAGAA CCAGTCTAGT CAAGCCAGCA ACCTGAATGT GTTGGCCGTA 
GATCAGAACA CGTCGGCAAG CTCCCAGCCC GACAACACAT CCCTGCTTGA TACCTCGGGG 
GTCGGGTTTG TGGTGGGAGA AGACCCAGAA ACTATCTCAA AGTCAGTGGA AGAAGAATTG 
AACAGAAAAA AAGAAAAGTT TATACAAATG CAGATCAAAC GCAAAGAAGA ACAAGACAAA 
AAACGTCAAT ATAAAGAGTT GGAAATGTCA CGCAGGAAAG AGCAAGAGAG AATTAAACAA 
GAAGAGGCAG AAAAACGTAA AGCCAGTGAA AAGGCACGAA GAGATGAAAT ATTCCGTCAG 
TACCAGGATA AGAAGCATCA AGAGGAAGAC AACTACGCAC CTCCTGTCCG CAGGAACCGA 
TCCAAAACCC GGGAAAAGAC CCGCCCCAAA TCCATGTTCG ACAAAGGAGA ATCAGGATCA 
GTGGAAGGTT TGCACTCCCA TAGTAGTTCT CAAGAGGATC TCTCTTCTCA AGCTTTTACT 
ATCAGTCCGG GTCACAAAGA ACCTTCGGGT GTTGGTCCTA GAAAACCCCC AAGCTTAGCA 
CCACGCCCAG GAAACATTCG AAAAGCAGTG TCCTGTAACA CCCTGTCCCA ATCCACGGAG 
GGCAAGCTGA CCTACAGGAG ACAGCCTTCT CCAGATCTTG GTCGTTTGAA GAGAGGCAGG 
AACAGTGCAG AGAGCAGTGA ATCTGGATCA GATTATGCAG GACCCAAGTT GTTTGTGAAG 
CCAAGCTCCA AGTCAAACCG CCACATCATC ATCAACGCCA TTAGTCAGTG TGTGCTAGCT 
GGACCCGTGA ACTCCGATCA GAAAAACAAA GTATTGGAGG TTTTGGCTAA ATCAGATGCC 
AAGCACTTCC TGATATTGTT CCGAGACCAC TCCTGTCAGT TCCGGGGACT GTACAGCTTT 
GATCCAGATT CAGAGGACTC CATTAAAATC CATGGAATAG GACCCAAACA AATCACCAGC 
AAAATGATCG AAAGATTTTA CAAGTACAAT TCTGGTGGAA AAAGCTTTAG TGAGGTTACA 
TCTACGAAAC ATTTATCAGT GTCAATAGAC GCAGTAGTTA TACACAATGC CTTGTGGAAA 
ACATCAGCAA AGCCGCCACA AAAAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105342461 ( XM_011449422.2 ) cDNA ORF clone, Crassostrea gigas(Pacific oyster) -> XP_011447724.1 Crassostrea gigas calmodulin-regulated spectrin-associated protein 1 (LOC105342461), transcript variant X2, mRNA.

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CloneID	OCr109220
Clone ID Related Accession (Same CDS sequence)	XM_011449422.2
Accession Version	XM_011449422.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-01
Organism	Crassostrea gigas(Pacific oyster)
Product	calmodulin-regulated spectrin-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011936675.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 2, 2017 this sequence version replaced XM_011449422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crassostrea gigas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAAAAT CATCAAACAT GAATCAGTCT GTGGAAAGTG CCCCTCCTGT TGATGACATC 
ATCGAAATCA TACCCATGGA CGAGTATGAT AGCAACAAGG CAAAACTTCA GTGCTCTGTA 
TCATGGATTC TCGACAAGGC CTACAGAGAT CAAATACCAA AGGAATTCAA AGACCCATTT 
TACGAGACAC AGGATGGTGT GATGCATATT AAGCCCAAGC TGATGAATCT GATGGTTTCC 
AGTGAGCTTT ACTGTCAGGC CTGCAATAAT ATGTTCGGGG ATTCATCATT ACAATGGCAA 
GGGACATCAG GTCATTCCAG CATCATTCAA GTGATGTCCA GAAAAGGGAT ATATGTTGTT 
GATGATAACG ATAAAGCTGT TACAGAGTCT GTACTTATAA AGTCGGCTCC CTTTGATGTG 
AGTGCACACA TTGCCCTGAT CGAAGCCTTG ATGCTGGCCT ACGTTAGACA GATAGTGACG 
GTGGAGAAAG TGGTACAAAC CGTCCGAAGG TTCGCTGCCT TCAATGCCTC CAGTGAGCTG 
CCGTCCAGTG TGGAGGATGC TCTCCTGTTC TGGATAAACA AAGTCTGTGT GGCCGTACAG 
CTCAGTGTGA ACAAGGAGGA TAACCAGGTG CTGGAAGGGG AGACAAATCA GAAGGTTCGT 
ATTGTGAGTA AGTCTGGGAT GAGCATGAGG GAGTCTGTGA ATATTCCACT GATAGAGGAC 
CTCTCTCAGG ACATTGGTGA TGGCTGTTCT CTGGCTGTTC TAATCAGCTT CTACTGCCCC 
TCCGCCCTCA GTCAGCAAGA TATCTGTTTA AAGTCTAATG TAGGCATAGC TGACTCGCTA 
TATAACCTTC GCCTAGTGAA GGAATTCTGT GATAACTACA TCCCTGGCAA AGCCTTCCAT 
CTGACCTACG AAGACTTCCT GTACATGCAC AACAACCTGC GACAGAACGT GGAGGTGTTT 
CTCGCAGAGC TGTTCTACTG GTTTGAGATT CAGAAGCTGG ACTGTGTGTA TAGTGAGCCA 
GGTTCGCGGC CCACAACAGC CAAGAAAATG ACCAGCAGCG CCATCACAAA TCTTCCTCCG 
ATCAGTAACG TCACCAAGCA GAGTTTCCAC AAGTACTCTG CGGATGATGG GGCGGTTCCT 
CGCGGAGTGA GCCAGATGCC ACCAAGACAA CCCCTCCTGT ATAAACGGCA GCAGGAACCT 
GATGTCAGTC AGCAAGGTCC AGGTGTGGGA CGATCAAGAC CAATGTCTGC TGTGGTCGGG 
AAGGAGAAGA ATGATGTACA TCAGTCAGTG CTGGCCTGGC AGGAGAACAG TCAACAGACT 
GTTCAGCAGA GTGGTGGTAG TAACCTCCTG GCCAATGTCA GCATTGACTC GGAGTTGATG 
GAGAGCTTTT CTGATGATCA GCTGGGAAGT CTCGACATGG ATCTGAGTGA CACCCCCCGG 
CCCACTCTGC CAGCCAATCA AGTGCCAAGA GAATCAGCAT CAAATCTACG TGATCCAAAT 
CATCCGGATT ATATGGAGGT ACAAAGTGTG ACATCAGGAG CTGCCACAAA TAGAACTGTT 
CATCAAGGGG AGGAAACTAG ACCTGATAGT GAGTCTGCTA TACCTGAGAA TCCTCCTTGT 
CATCAAGACT TGCTAAATGA ATTCAGTGCT CAATTAGACC CTAAAGACAA TAAGCAGAGT 
AGAAATGAAT GCTATGAGCC TTTACTACCT GCAAAATTAA AACCTCGGAA AGAAGCAATA 
AATAACCACA GCAAGGAACA AGAGAGGGGA GATAAGTCTT TACTTGCTAA ATCAGTGAAA 
TCTATGGAAG TGGATCTGGG TTCTTCAAGT GTTTTAAGTG AGCGAAGTGA CAGCAGTAGT 
TCTGTTCCCG TCACTCCTCT ACCCGAGGAT CAGTTATCGG ACCAAGATGT TCCCAAACAG 
CCCCGTTATA CAGCATTTAC AGTCAACACA GGCTTTTCAG AAGACATGGA ATCTCCACGA 
AAAAGTGTAC TGTCCACCAT CCGTAGCCAA GGCGATACTG CTGAATCAGT TAAACTGAGT 
TATACTGTCT CCAATGAGGC CACTTCAGAA TCGGCGCGGG CAGCCGGGAT ACCTGTTGTC 
GGGGAGAGTG GCGAACAAGC ATGGAGGAGA TGGGGCTCCA GAGAGGGTAG TGTGAACAGT 
AGGAGTTCTG GGGACAATTC CGATCATGAA TCTCAGAAAA TTCACTACGA CCAGAAACTG 
AGTGAGAACA AAGACAAAAT CCGAGAGGTG TCACCTCCAA AAACTTTTAC TAGATTCGGT 
TCCAAGGACA GTGATGTATT TCAAAAACCG AAAATGATAG TTAGAAGTAA GACAGCTGTT 
TCACCAAAGA AAGAACCCTC CAAACATATG ACAACAAATT TTGCTGAAAT AAAGAGAATG 
AAGAATGTTC AAGGGAATGT TGACAATTCT GGAATGGTTT ACATGCAGCA TGGCCATGAA 
GTGAATAACA AAGCTATGCA AAGCTCAACA ACTCAAATCA ACAATAGGAA CAACCAAAAC 
TCAACGTCAC CTAGCAGAAC AACATGGCAG CAAGCAAGCA CTCTAAAATC TCCTGATCAG 
GCCACAAGTG ATTCCTCAGA AAGCAGTGAC AGTAGTCGCC CCGTGCTGTC TGAGCTAACA 
GAAATCCGGC TTAAGTTGGA GGAAAAGCGT AAACAGATAG AGAAAAAGAA GAACAGAATG 
GAGCTACAGC AACAGAAACA GAGACAGCGG CTAGGGAAGA CAGCTTTTCT TCATGTGCTG 
ACAAAACCCA CTGAGGAGGA GGTGTCTGAT GACTTGAGTT CACAGGGAAG TGAGGCGTCT 
CTACCAGCAA GTGAGGAGGC TGCCTCAAGT TCAACCTCGG ACAAAGGACC AACAGCAGCA 
GATGGAAAAA CTACAGAAGC ACCTCAAAGC AACAAAGCAT TTTCTAGGGA GGGGATACAG 
CAAACAATAG AAAATGTTAG AAAGAAGTGG TTTCAAGAGG ACAAGGATGA GCAACCTGTG 
CAGATGGAGA TAAAACCAGA AGAAATAATG AGTAAGAGTT GTCCAGCCAA AAATGGAGAA 
GTTTTACAAA AACAAAAATC GGCGCTGCCT GCCCAGGAGC AAAATTCAAT GTCAATGATA 
GAAAATATAT CTGACAGAAA ATTATCTGAA GCTCAAGAAT TGGACAGTCC ATCTGTATAC 
AGTCGGAAGC TTGAAAAACT GGATCATGAC CTGACTGATT TGAAAGGGGA GATCCTGAGG 
ATGTCTCTGG AACAGGAGCA GTACAATGGG AGTCCAGCGG CTACTTCCAT GACACACCCA 
TCCACAGCCA CCAGGCCAAC TTATGCAGTG GAAACTCCAG AACGGAAATT CCAGGGAAAA 
CCATTCCATG GATCAGATCT CGCCTCAGTG AATCAGGCAA ACGTAAACAC TCCAGAGAGA 
ATGCAGTATG GAACCCCGGC GGTTTGTCGC CATGCAGGAT ATGCAGGCCC CATTGATCCC 
AGTGTACCCA CCCCAGAGAG GGTTGTGATG GGGGGAGGTA TGTCTCAGTA CCCCCAGGGC 
CTACCCCCAC AGATGCCTAT TACACAACCA TTCCAGCCCC AAGTGATGCC CCACTCTTTA 
CCCTATAATG GACAGAGTCA GTTCAGTCCA ATGCCACAAA CACAGGTGCC ATACAGCGGT 
TTCCAAACTG TTGGTACTAC TCCCTATCCA CAACCCCAGT TCCCTCCCAC CCCACCCCAT 
GGCTACCAAC AGTCCCCTCC CTTCAATCCC AATCTGGTGT CACCCCACGG TCAATATGGG 
ATGCCTGGGG TATATAATAT GACTCCTCAG CAGTACCCTC CCCATTCTCA CAACACATCT 
ACAGTGTCAG CTCATACTCC TGTCTCTTAC ATGTACTCGC CCAATCACAC CGGAGCTCAA 
GTCTTTCAAC CTTCTGCGGA TATTAATAAA ATGCCGCCTG CTTCTCGGGC TGAAATGCCC 
CCCACTTCTA GTGCTTTCAA TGATGGTAAC ATCACCAAAG AAGACACCTC TGCTGTTTCA 
AATATGGGTG TTCATGACTT GTCTCATAAC AAAAGTGAAA TACAAAGTGA CATTTCTAGT 
GACCAGAATG GATTTTTTGT GTCATTTGGA GACAATTCTC CAAAGGTTAA ACCCAAACTT 
TCTAGTGCAA CATCTCAAAA GACGGTAACC TCTGAACCAA CTAGTCTCCA AAACCAAACA 
CAACAGTCAC CTCAGCAGAA CCAGTCTAGT CAAGCCAGCA ACCTGAATGT GTTGGCCGTA 
GATCAGAACA CGTCGGCAAG CTCCCAGCCC GACAACACAT CCCTGCTTGA TACCTCGGGG 
GTCGGGTTTG TGGTGGGAGA AGACCCAGAA ACTATCTCAA AGTCAGTGGA AGAAGAATTG 
AACAGAAAAA AAGAAAAGTT TATACAAATG CAGATCAAAC GCAAAGAAGA ACAAGACAAA 
AAACGTCAAT ATAAAGAGTT GGAAATGTCA CGCAGGAAAG AGCAAGAGAG AATTAAACAA 
GAAGAGGCAG AAAAACGTAA AGCCAGTGAA AAGGCACGAA GAGATGAAAT ATTCCGTCAG 
TACCAGGATA AGAAGCATCA AGAGGAAGAC AACTACGCAC CTCCTGTCCG CAGGAACCGA 
TCCAAAACCC GGGAAAAGAC CCGCCCCAAA TCCATGTTCG ACAAAGGAGA ATCAGGATCA 
GTGGAAGGTT TGCACTCCCA TAGTAGTTCT CAAGAGGATC TCTCTTCTCA AGCTTTTACT 
ATCAGTCCGG GTCACAAAGA ACCTTTGTCC TGTAACACCC TGTCCCAATC CACGGAGGGC 
AAGCTGACCT ACAGGAGACA GCCTTCTCCA GATCTTGGTC GTTTGAAGAG AGGCAGGAAC 
AGTGCAGAGA GCAGTGAATC TGGATCAGAT TATGCAGGAC CCAAGTTGTT TGTGAAGCCA 
AGCTCCAAGT CAAACCGCCA CATCATCATC AACGCCATTA GTCAGTGTGT GCTAGCTGGA 
CCCGTGAACT CCGATCAGAA AAACAAAGTA TTGGAGGTTT TGGCTAAATC AGATGCCAAG 
CACTTCCTGA TATTGTTCCG AGACCACTCC TGTCAGTTCC GGGGACTGTA CAGCTTTGAT 
CCAGATTCAG AGGACTCCAT TAAAATCCAT GGAATAGGAC CCAAACAAAT CACCAGCAAA 
ATGATCGAAA GATTTTACAA GTACAATTCT GGTGGAAAAA GCTTTAGTGA GGTTACATCT 
ACGAAACATT TATCAGTGTC AATAGACGCA GTAGTTATAC ACAATGCCTT GTGGAAAACA 
TCAGCAAAGC CGCCACAAAA AAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011447724.1
CDS	229..5535
Translation	MIKSSNMNQSVESAPPVDDIIEIIPMDEYDSNKAKLQCSVSWILDKAYRDQIPKEFKDPFYETQDGVMHIKPKLMNLMVSSELYCQACNNMFGDSSLQWQGTSGHSSIIQVMSRKGIYVVDDNDKAVTESVLIKSAPFDVSAHIALIEALMLAYVRQIVTVEKVVQTVRRFAAFNASSELPSSVEDALLFWINKVCVAVQLSVNKEDNQVLEGETNQKVRIVSKSGMSMRESVNIPLIEDLSQDIGDGCSLAVLISFYCPSALSQQDICLKSNVGIADSLYNLRLVKEFCDNYIPGKAFHLTYEDFLYMHNNLRQNVEVFLAELFYWFEIQKLDCVYSEPGSRPTTAKKMTSSAITNLPPISNVTKQSFHKYSADDGAVPRGVSQMPPRQPLLYKRQQEPDVSQQGPGVGRSRPMSAVVGKEKNDVHQSVLAWQENSQQTVQQSGGSNLLANVSIDSELMESFSDDQLGSLDMDLSDTPRPTLPANQVPRESASNLRDPNHPDYMEVQSVTSGAATNRTVHQGEETRPDSESAIPENPPCHQDLLNEFSAQLDPKDNKQSRNECYEPLLPAKLKPRKEAINNHSKEQERGDKSLLAKSVKSMEVDLGSSSVLSERSDSSSSVPVTPLPEDQLSDQDVPKQPRYTAFTVNTGFSEDMESPRKSVLSTIRSQGDTAESVKLSYTVSNEATSESARAAGIPVVGESGEQAWRRWGSREGSVNSRSSGDNSDHESQKIHYDQKLSENKDKIREVSPPKTFTRFGSKDSDVFQKPKMIVRSKTAVSPKKEPSKHMTTNFAEIKRMKNVQGNVDNSGMVYMQHGHEVNNKAMQSSTTQINNRNNQNSTSPSRTTWQQASTLKSPDQATSDSSESSDSSRPVLSELTEIRLKLEEKRKQIEKKKNRMELQQQKQRQRLGKTAFLHVLTKPTEEEVSDDLSSQGSEASLPASEEAASSSTSDKGPTAADGKTTEAPQSNKAFSREGIQQTIENVRKKWFQEDKDEQPVQMEIKPEEIMSKSCPAKNGEVLQKQKSALPAQEQNSMSMIENISDRKLSEAQELDSPSVYSRKLEKLDHDLTDLKGEILRMSLEQEQYNGSPAATSMTHPSTATRPTYAVETPERKFQGKPFHGSDLASVNQANVNTPERMQYGTPAVCRHAGYAGPIDPSVPTPERVVMGGGMSQYPQGLPPQMPITQPFQPQVMPHSLPYNGQSQFSPMPQTQVPYSGFQTVGTTPYPQPQFPPTPPHGYQQSPPFNPNLVSPHGQYGMPGVYNMTPQQYPPHSHNTSTVSAHTPVSYMYSPNHTGAQVFQPSADINKMPPASRAEMPPTSSAFNDGNITKEDTSAVSNMGVHDLSHNKSEIQSDISSDQNGFFVSFGDNSPKVKPKLSSATSQKTVTSEPTSLQNQTQQSPQQNQSSQASNLNVLAVDQNTSASSQPDNTSLLDTSGVGFVVGEDPETISKSVEEELNRKKEKFIQMQIKRKEEQDKKRQYKELEMSRRKEQERIKQEEAEKRKASEKARRDEIFRQYQDKKHQEEDNYAPPVRRNRSKTREKTRPKSMFDKGESGSVEGLHSHSSSQEDLSSQAFTISPGHKEPLSCNTLSQSTEGKLTYRRQPSPDLGRLKRGRNSAESSESGSDYAGPKLFVKPSSKSNRHIIINAISQCVLAGPVNSDQKNKVLEVLAKSDAKHFLILFRDHSCQFRGLYSFDPDSEDSIKIHGIGPKQITSKMIERFYKYNSGGKSFSEVTSTKHLSVSIDAVVIHNALWKTSAKPPQKR

Target ORF information:

RefSeq Version	XM_011449422.2
Organism	Crassostrea gigas(Pacific oyster)
Definition	Crassostrea gigas calmodulin-regulated spectrin-associated protein 1 (LOC105342461), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011449422.2

ORF Insert Sequence:

ATGATAAAAT CATCAAACAT GAATCAGTCT GTGGAAAGTG CCCCTCCTGT TGATGACATC 
ATCGAAATCA TACCCATGGA CGAGTATGAT AGCAACAAGG CAAAACTTCA GTGCTCTGTA 
TCATGGATTC TCGACAAGGC CTACAGAGAT CAAATACCAA AGGAATTCAA AGACCCATTT 
TACGAGACAC AGGATGGTGT GATGCATATT AAGCCCAAGC TGATGAATCT GATGGTTTCC 
AGTGAGCTTT ACTGTCAGGC CTGCAATAAT ATGTTCGGGG ATTCATCATT ACAATGGCAA 
GGGACATCAG GTCATTCCAG CATCATTCAA GTGATGTCCA GAAAAGGGAT ATATGTTGTT 
GATGATAACG ATAAAGCTGT TACAGAGTCT GTACTTATAA AGTCGGCTCC CTTTGATGTG 
AGTGCACACA TTGCCCTGAT CGAAGCCTTG ATGCTGGCCT ACGTTAGACA GATAGTGACG 
GTGGAGAAAG TGGTACAAAC CGTCCGAAGG TTCGCTGCCT TCAATGCCTC CAGTGAGCTG 
CCGTCCAGTG TGGAGGATGC TCTCCTGTTC TGGATAAACA AAGTCTGTGT GGCCGTACAG 
CTCAGTGTGA ACAAGGAGGA TAACCAGGTG CTGGAAGGGG AGACAAATCA GAAGGTTCGT 
ATTGTGAGTA AGTCTGGGAT GAGCATGAGG GAGTCTGTGA ATATTCCACT GATAGAGGAC 
CTCTCTCAGG ACATTGGTGA TGGCTGTTCT CTGGCTGTTC TAATCAGCTT CTACTGCCCC 
TCCGCCCTCA GTCAGCAAGA TATCTGTTTA AAGTCTAATG TAGGCATAGC TGACTCGCTA 
TATAACCTTC GCCTAGTGAA GGAATTCTGT GATAACTACA TCCCTGGCAA AGCCTTCCAT 
CTGACCTACG AAGACTTCCT GTACATGCAC AACAACCTGC GACAGAACGT GGAGGTGTTT 
CTCGCAGAGC TGTTCTACTG GTTTGAGATT CAGAAGCTGG ACTGTGTGTA TAGTGAGCCA 
GGTTCGCGGC CCACAACAGC CAAGAAAATG ACCAGCAGCG CCATCACAAA TCTTCCTCCG 
ATCAGTAACG TCACCAAGCA GAGTTTCCAC AAGTACTCTG CGGATGATGG GGCGGTTCCT 
CGCGGAGTGA GCCAGATGCC ACCAAGACAA CCCCTCCTGT ATAAACGGCA GCAGGAACCT 
GATGTCAGTC AGCAAGGTCC AGGTGTGGGA CGATCAAGAC CAATGTCTGC TGTGGTCGGG 
AAGGAGAAGA ATGATGTACA TCAGTCAGTG CTGGCCTGGC AGGAGAACAG TCAACAGACT 
GTTCAGCAGA GTGGTGGTAG TAACCTCCTG GCCAATGTCA GCATTGACTC GGAGTTGATG 
GAGAGCTTTT CTGATGATCA GCTGGGAAGT CTCGACATGG ATCTGAGTGA CACCCCCCGG 
CCCACTCTGC CAGCCAATCA AGTGCCAAGA GAATCAGCAT CAAATCTACG TGATCCAAAT 
CATCCGGATT ATATGGAGGT ACAAAGTGTG ACATCAGGAG CTGCCACAAA TAGAACTGTT 
CATCAAGGGG AGGAAACTAG ACCTGATAGT GAGTCTGCTA TACCTGAGAA TCCTCCTTGT 
CATCAAGACT TGCTAAATGA ATTCAGTGCT CAATTAGACC CTAAAGACAA TAAGCAGAGT 
AGAAATGAAT GCTATGAGCC TTTACTACCT GCAAAATTAA AACCTCGGAA AGAAGCAATA 
AATAACCACA GCAAGGAACA AGAGAGGGGA GATAAGTCTT TACTTGCTAA ATCAGTGAAA 
TCTATGGAAG TGGATCTGGG TTCTTCAAGT GTTTTAAGTG AGCGAAGTGA CAGCAGTAGT 
TCTGTTCCCG TCACTCCTCT ACCCGAGGAT CAGTTATCGG ACCAAGATGT TCCCAAACAG 
CCCCGTTATA CAGCATTTAC AGTCAACACA GGCTTTTCAG AAGACATGGA ATCTCCACGA 
AAAAGTGTAC TGTCCACCAT CCGTAGCCAA GGCGATACTG CTGAATCAGT TAAACTGAGT 
TATACTGTCT CCAATGAGGC CACTTCAGAA TCGGCGCGGG CAGCCGGGAT ACCTGTTGTC 
GGGGAGAGTG GCGAACAAGC ATGGAGGAGA TGGGGCTCCA GAGAGGGTAG TGTGAACAGT 
AGGAGTTCTG GGGACAATTC CGATCATGAA TCTCAGAAAA TTCACTACGA CCAGAAACTG 
AGTGAGAACA AAGACAAAAT CCGAGAGGTG TCACCTCCAA AAACTTTTAC TAGATTCGGT 
TCCAAGGACA GTGATGTATT TCAAAAACCG AAAATGATAG TTAGAAGTAA GACAGCTGTT 
TCACCAAAGA AAGAACCCTC CAAACATATG ACAACAAATT TTGCTGAAAT AAAGAGAATG 
AAGAATGTTC AAGGGAATGT TGACAATTCT GGAATGGTTT ACATGCAGCA TGGCCATGAA 
GTGAATAACA AAGCTATGCA AAGCTCAACA ACTCAAATCA ACAATAGGAA CAACCAAAAC 
TCAACGTCAC CTAGCAGAAC AACATGGCAG CAAGCAAGCA CTCTAAAATC TCCTGATCAG 
GCCACAAGTG ATTCCTCAGA AAGCAGTGAC AGTAGTCGCC CCGTGCTGTC TGAGCTAACA 
GAAATCCGGC TTAAGTTGGA GGAAAAGCGT AAACAGATAG AGAAAAAGAA GAACAGAATG 
GAGCTACAGC AACAGAAACA GAGACAGCGG CTAGGGAAGA CAGCTTTTCT TCATGTGCTG 
ACAAAACCCA CTGAGGAGGA GGTGTCTGAT GACTTGAGTT CACAGGGAAG TGAGGCGTCT 
CTACCAGCAA GTGAGGAGGC TGCCTCAAGT TCAACCTCGG ACAAAGGACC AACAGCAGCA 
GATGGAAAAA CTACAGAAGC ACCTCAAAGC AACAAAGCAT TTTCTAGGGA GGGGATACAG 
CAAACAATAG AAAATGTTAG AAAGAAGTGG TTTCAAGAGG ACAAGGATGA GCAACCTGTG 
CAGATGGAGA TAAAACCAGA AGAAATAATG AGTAAGAGTT GTCCAGCCAA AAATGGAGAA 
GTTTTACAAA AACAAAAATC GGCGCTGCCT GCCCAGGAGC AAAATTCAAT GTCAATGATA 
GAAAATATAT CTGACAGAAA ATTATCTGAA GCTCAAGAAT TGGACAGTCC ATCTGTATAC 
AGTCGGAAGC TTGAAAAACT GGATCATGAC CTGACTGATT TGAAAGGGGA GATCCTGAGG 
ATGTCTCTGG AACAGGAGCA GTACAATGGG AGTCCAGCGG CTACTTCCAT GACACACCCA 
TCCACAGCCA CCAGGCCAAC TTATGCAGTG GAAACTCCAG AACGGAAATT CCAGGGAAAA 
CCATTCCATG GATCAGATCT CGCCTCAGTG AATCAGGCAA ACGTAAACAC TCCAGAGAGA 
ATGCAGTATG GAACCCCGGC GGTTTGTCGC CATGCAGGAT ATGCAGGCCC CATTGATCCC 
AGTGTACCCA CCCCAGAGAG GGTTGTGATG GGGGGAGGTA TGTCTCAGTA CCCCCAGGGC 
CTACCCCCAC AGATGCCTAT TACACAACCA TTCCAGCCCC AAGTGATGCC CCACTCTTTA 
CCCTATAATG GACAGAGTCA GTTCAGTCCA ATGCCACAAA CACAGGTGCC ATACAGCGGT 
TTCCAAACTG TTGGTACTAC TCCCTATCCA CAACCCCAGT TCCCTCCCAC CCCACCCCAT 
GGCTACCAAC AGTCCCCTCC CTTCAATCCC AATCTGGTGT CACCCCACGG TCAATATGGG 
ATGCCTGGGG TATATAATAT GACTCCTCAG CAGTACCCTC CCCATTCTCA CAACACATCT 
ACAGTGTCAG CTCATACTCC TGTCTCTTAC ATGTACTCGC CCAATCACAC CGGAGCTCAA 
GTCTTTCAAC CTTCTGCGGA TATTAATAAA ATGCCGCCTG CTTCTCGGGC TGAAATGCCC 
CCCACTTCTA GTGCTTTCAA TGATGGTAAC ATCACCAAAG AAGACACCTC TGCTGTTTCA 
AATATGGGTG TTCATGACTT GTCTCATAAC AAAAGTGAAA TACAAAGTGA CATTTCTAGT 
GACCAGAATG GATTTTTTGT GTCATTTGGA GACAATTCTC CAAAGGTTAA ACCCAAACTT 
TCTAGTGCAA CATCTCAAAA GACGGTAACC TCTGAACCAA CTAGTCTCCA AAACCAAACA 
CAACAGTCAC CTCAGCAGAA CCAGTCTAGT CAAGCCAGCA ACCTGAATGT GTTGGCCGTA 
GATCAGAACA CGTCGGCAAG CTCCCAGCCC GACAACACAT CCCTGCTTGA TACCTCGGGG 
GTCGGGTTTG TGGTGGGAGA AGACCCAGAA ACTATCTCAA AGTCAGTGGA AGAAGAATTG 
AACAGAAAAA AAGAAAAGTT TATACAAATG CAGATCAAAC GCAAAGAAGA ACAAGACAAA 
AAACGTCAAT ATAAAGAGTT GGAAATGTCA CGCAGGAAAG AGCAAGAGAG AATTAAACAA 
GAAGAGGCAG AAAAACGTAA AGCCAGTGAA AAGGCACGAA GAGATGAAAT ATTCCGTCAG 
TACCAGGATA AGAAGCATCA AGAGGAAGAC AACTACGCAC CTCCTGTCCG CAGGAACCGA 
TCCAAAACCC GGGAAAAGAC CCGCCCCAAA TCCATGTTCG ACAAAGGAGA ATCAGGATCA 
GTGGAAGGTT TGCACTCCCA TAGTAGTTCT CAAGAGGATC TCTCTTCTCA AGCTTTTACT 
ATCAGTCCGG GTCACAAAGA ACCTTTGTCC TGTAACACCC TGTCCCAATC CACGGAGGGC 
AAGCTGACCT ACAGGAGACA GCCTTCTCCA GATCTTGGTC GTTTGAAGAG AGGCAGGAAC 
AGTGCAGAGA GCAGTGAATC TGGATCAGAT TATGCAGGAC CCAAGTTGTT TGTGAAGCCA 
AGCTCCAAGT CAAACCGCCA CATCATCATC AACGCCATTA GTCAGTGTGT GCTAGCTGGA 
CCCGTGAACT CCGATCAGAA AAACAAAGTA TTGGAGGTTT TGGCTAAATC AGATGCCAAG 
CACTTCCTGA TATTGTTCCG AGACCACTCC TGTCAGTTCC GGGGACTGTA CAGCTTTGAT 
CCAGATTCAG AGGACTCCAT TAAAATCCAT GGAATAGGAC CCAAACAAAT CACCAGCAAA 
ATGATCGAAA GATTTTACAA GTACAATTCT GGTGGAAAAA GCTTTAGTGA GGTTACATCT 
ACGAAACATT TATCAGTGTC AATAGACGCA GTAGTTATAC ACAATGCCTT GTGGAAAACA 
TCAGCAAAGC CGCCACAAAA AAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105342461 ( XM_011449423.2 ) cDNA ORF clone, Crassostrea gigas(Pacific oyster) -> XP_011447725.1 Crassostrea gigas calmodulin-regulated spectrin-associated protein 1 (LOC105342461), transcript variant X3, mRNA.

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CloneID	OCr109221
Clone ID Related Accession (Same CDS sequence)	XM_011449423.2
Accession Version	XM_011449423.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-01
Organism	Crassostrea gigas(Pacific oyster)
Product	calmodulin-regulated spectrin-associated protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011936675.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 2, 2017 this sequence version replaced XM_011449423.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Crassostrea gigas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATAAAAT CATCAAACAT GAATCAGTCT GTGGAAAGTG CCCCTCCTGT TGATGACATC 
ATCGAAATCA TACCCATGGA CGAGTATGAT AGCAACAAGG CAAAACTTCA GTGCTCTGTA 
TCATGGATTC TCGACAAGGC CTACAGAGAT CAAATACCAA AGGAATTCAA AGACCCATTT 
TACGAGACAC AGGATGGTGT GATGCATATT AAGCCCAAGC TGATGAATCT GATGGTTTCC 
AGTGAGCTTT ACTGTCAGGC CTGCAATAAT ATGTTCGGGG ATTCATCATT ACAATGGCAA 
GGGACATCAG GTCATTCCAG CATCATTCAA GTGATGTCCA GAAAAGGGAT ATATGTTGTT 
GATGATAACG ATAAAGCTGT TACAGAGTCT GTACTTATAA AGTCGGCTCC CTTTGATGTG 
AGTGCACACA TTGCCCTGAT CGAAGCCTTG ATGCTGGCCT ACGTTAGACA GATAGTGACG 
GTGGAGAAAG TGGTACAAAC CGTCCGAAGG TTCGCTGCCT TCAATGCCTC CAGTGAGCTG 
CCGTCCAGTG TGGAGGATGC TCTCCTGTTC TGGATAAACA AAGTCTGTGT GGCCGTACAG 
CTCAGTGTGA ACAAGGAGGA TAACCAGGTG CTGGAAGGGG AGACAAATCA GAAGGTTCGT 
ATTGTGAGTA AGTCTGGGAT GAGCATGAGG GAGTCTGTGA ATATTCCACT GATAGAGGAC 
CTCTCTCAGG ACATTGGTGA TGGCTGTTCT CTGGCTGTTC TAATCAGCTT CTACTGCCCC 
TCCGCCCTCA GTCAGCAAGA TATCTGTTTA AAGTCTAATG TAGGCATAGC TGACTCGCTA 
TATAACCTTC GCCTAGTGAA GGAATTCTGT GATAACTACA TCCCTGGCAA AGCCTTCCAT 
CTGACCTACG AAGACTTCCT GTACATGCAC AACAACCTGC GACAGAACGT GGAGGTGTTT 
CTCGCAGAGC TGTTCTACTG GTTTGAGATT CAGAAGCTGG ACTGTGTGTA TAGTGAGCCA 
GGTTCGCGGC CCACAACAGC CAAGAAAATG ACCAGCAGCG CCATCACAAA TCTTCCTCCG 
ATCAGTAACG TCACCAAGCA GAGTTTCCAC AAGTACTCTG CGGATGATGG GGCGGTTCCT 
CGCGGAGTGA GCCAGATGCC ACCAAGACAA CCCCTCCTGT ATAAACGGCA GCAGGAACCT 
GATGTCAGTC AGCAAGGTCC AGGTGTGGGA CGATCAAGAC CAATGTCTGC TGTGGTCGGG 
AAGGAGAAGA ATGATGTACA TCAGTCAGTG CTGGCCTGGC AGGAGAACAG TCAACAGACT 
GTTCAGCAGA GTGGTGGTAG TAACCTCCTG GCCAATGTCA GCATTGACTC GGAGTTGATG 
GAGAGCTTTT CTGATGATCA GCTGGGAAGT CTCGACATGG ATCTGAGTGA CACCCCCCGG 
CCCACTCTGC CAGCCAATCA AGTGCCAAGA GAATCAGCAT CAAATCTACG TGATCCAAAT 
CATCCGGATT ATATGGAGGT ACAAAGTGTG ACATCAGGAG CTGCCACAAA TAGAACTGTT 
CATCAAGGGG AGGAAACTAG ACCTGATAGT GAGTCTGCTA TACCTGAGAA TCCTCCTTGT 
CATCAAGACT TGCTAAATGA ATTCAGTGCT CAATTAGACC CTAAAGACAA TAAGCAGAGT 
AGAAATGAAT GCTATGAGCC TTTACTACCT GCAAAATTAA AACCTCGGAA AGAAGCAATA 
AATAACCACA GCAAGGAACA AGAGAGGGGA GATAAGTCTT TACTTGCTAA ATCAGTGAAA 
TCTATGGAAG TGGATCTGGG TTCTTCAAGT GTTTTAAGTG AGCGAAGTGA CAGCAGTAGT 
TCTGTTCCCG TCACTCCTCT ACCCGAGGAT CAGTTATCGG ACCAAGATGT TCCCAAACAG 
CCCCGTTATA CAGCATTTAC AGTCAACACA GGCTTTTCAG AAGACATGGA ATCTCCACGA 
AAAAGTGTAC TGTCCACCAT CCGTAGCCAA GGCGATACTG CTGAATCAGT TAAACTGAGT 
TATACTGTCT CCAATGAGGC CACTTCAGAA TCGGCGCGGG CAGCCGGGAT ACCTGTTGTC 
GGGGAGAGTG GCGAACAAGC ATGGAGGAGA TGGGGCTCCA GAGAGGGTAG TGTGAACAGT 
AGGAGTTCTG GGGACAATTC CGATCATGAA TCTCAGAAAA TTCACTACGA CCAGAAACTG 
AGTGAGAACA AAGACAAAAT CCGAGAGGTG TCACCTCCAA AAACTTTTAC TAGATTCGGT 
TCCAAGGACA GTGATGTATT TCAAAAACCG AAAATGATAG TTAGAAGTAA GACAGCTGTT 
TCACCAAAGA AAGAACCCTC CAAACATATG ACAACAAATT TTGCTGAAAT AAAGAGAATG 
AAGAATGTTC AAGGGAATGT TGACAATTCT GGAATGGTTT ACATGCAGCA TGGCCATGAA 
GTGAATAACA AAGCTATGCA AAGCTCAACA ACTCAAATCA ACAATAGGAA CAACCAAAAC 
TCAACGTCAC CTAGCAGAAC AACATGGCAG CAAGCAAGCA CTCTAAAATC TCCTGATCAG 
GCCACAAGTG ATTCCTCAGA AAGCAGTGAC AGTAGTCGCC CCGTGCTGTC TGAGCTAACA 
GAAATCCGGC TTAAGTTGGA GGAAAAGCGT AAACAGATAG AGAAAAAGAA GAACAGAATG 
GAGCTACAGC AACAGAAACA GAGACAGCGG CTAGGGAAGA CAGCTTTTCT TCATGTGCTG 
ACAAAACCCA CTGAGGAGGA GGTGTCTGAT GACTTGAGTT CACAGGGAAG TGAGGCGTCT 
CTACCAGCAA GTGAGGAGGC TGCCTCAAGT TCAACCTCGG ACAAAGGACC AACAGCAGCA 
GATGGAAAAA CTACAGAAGC ACCTCAAAGC AACAAAGCAT TTTCTAGGGA GGGGATACAG 
CAAACAATAG AAAATGTTAG AAAGAAGTGG TTTCAAGAGG ACAAGGATGA GCAACCTGTG 
CAGATGGAGA TAAAACCAGA AGAAATAATG AGTAAGAGTT GTCCAGCCAA AAATGGAGAA 
GTTTTACAAA AACAAAAATC GGCGCTGCCT GCCCAGGAGC AAAATTCAAT GTCAATGATA 
GAAAATATAT CTGACAGAAA ATTATCTGAA GCTCAAGAAT TGGACAGTCC ATCTGTATAC 
AGTCGGAAGC TTGAAAAACT GGATCATGAC CTGACTGATT TGAAAGGGGA GATCCTGAGG 
ATGTCTCTGG AACAGGAGCA GTACAATGGG AGTCCAGCGG CTACTTCCAT GACACACCCA 
TCCACAGCCA CCAGGCCAAC TTATGCAGTG GAAACTCCAG AACGGAAATT CCAGGGAAAA 
CCATTCCATG GATCAGATCT CGCCTCAGTG AATCAGGCAA ACGTAAACAC TCCAGAGAGA 
ATGCAGTATG GAACCCCGGC GGTTTGTCGC CATGCAGGAT ATGCAGGCCC CATTGATCCC 
AGTGTACCCA CCCCAGAGAG GGTTGTGATG GGGGGAGGTA TGTCTCAGTA CCCCCAGGGC 
CTACCCCCAC AGATGCCTAT TACACAACCA TTCCAGCCCC AAGTGATGCC CCACTCTTTA 
CCCTATAATG GACAGAGTCA GTTCAGTCCA ATGCCACAAA CACAGGTGCC ATACAGCGGT 
TTCCAAACTG TTGGTACTAC TCCCTATCCA CAACCCCAGT TCCCTCCCAC CCCACCCCAT 
GGCTACCAAC AGTCCCCTCC CTTCAATCCC AATCTGGTGT CACCCCACGG TCAATATGGG 
ATGCCTGGGG TATATAATAT GACTCCTCAG CAGTACCCTC CCCATTCTCA CAACACATCT 
ACAGTGTCAG CTCATACTCC TGTCTCTTAC ATGTACTCGC CCAATCACAC CGGAGCTCAA 
GTCTTTCAAC CTTCTGCGGA TATTAATAAA ATGCCGCCTG CTTCTCGGGC TGAAATGCCC 
CCCACTTCTA GTGCTTTCAA TGATGGTAAC ATCACCAAAG AAGACACCTC TGCTGTTTCA 
AATATGGGTG TTCATGACTT GTCTCATAAC AAAAGTGAAA TACAAAGTGA CATTTCTAGT 
GACCAGAATG GATTTTTTGT GTCATTTGGA GACAATTCTC CAAAGGTTAA ACCCAAACTT 
TCTAGTGCAA CATCTCAAAA GACGGTAACC TCTGAACCAA CTAGTCTCCA AAACCAAACA 
CAACAGTCAC CTCAGCAGAA CCAGTCTAGT CAAGCCAGCA ACCTGAATGT GTTGGCCGTA 
GATCAGAACA CGTCGGCAAG CTCCCAGCCC GACAACACAT CCCTGCTTGA TACCTCGGGG 
GTCGGGTTTG TGGTGGGAGA AGACCCAGAA ACTATCTCAA AGTCAGTGGA AGAAGAATTG 
AACAGAAAAA AAGAAAAGTT TATACAAATG CAGATCAAAC GCAAAGAAGA ACAAGACAAA 
AAACGTCAAT ATAAAGAGTT GGAAATGTCA CGCAGGAAAG AGCAAGAGAG AATTAAACAA 
GAAGAGGCAG AAAAACGTAA AGCCAGTGAA AAGGCACGAA GAGATGAAAT ATTCCGTCAG 
TACCAGGATA AGAAGCATCA AGAGGAAGAC AACTACGCAC CTCCTGTCCG CAGGAACCGA 
TCCAAAACCC GGGAAAAGAC CCGCCCCAAA TCCATGTTCG ACAAAGGAGA ATCAGGATCA 
GTGGAAGCGG GTGTTGGTCC TAGAAAACCC CCAAGCTTAG CACCACGCCC AGGAAACATT 
CGAAAAGCAG TGTCCTGTAA CACCCTGTCC CAATCCACGG AGGGCAAGCT GACCTACAGG 
AGACAGCCTT CTCCAGATCT TGGTCGTTTG AAGAGAGGCA GGAACAGTGC AGAGAGCAGT 
GAATCTGGAT CAGATTATGC AGGACCCAAG TTGTTTGTGA AGCCAAGCTC CAAGTCAAAC 
CGCCACATCA TCATCAACGC CATTAGTCAG TGTGTGCTAG CTGGACCCGT GAACTCCGAT 
CAGAAAAACA AAGTATTGGA GGTTTTGGCT AAATCAGATG CCAAGCACTT CCTGATATTG 
TTCCGAGACC ACTCCTGTCA GTTCCGGGGA CTGTACAGCT TTGATCCAGA TTCAGAGGAC 
TCCATTAAAA TCCATGGAAT AGGACCCAAA CAAATCACCA GCAAAATGAT CGAAAGATTT 
TACAAGTACA ATTCTGGTGG AAAAAGCTTT AGTGAGGTTA CATCTACGAA ACATTTATCA 
GTGTCAATAG ACGCAGTAGT TATACACAAT GCCTTGTGGA AAACATCAGC AAAGCCGCCA 
CAAAAAAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011447725.1
CDS	229..5520
Translation	MIKSSNMNQSVESAPPVDDIIEIIPMDEYDSNKAKLQCSVSWILDKAYRDQIPKEFKDPFYETQDGVMHIKPKLMNLMVSSELYCQACNNMFGDSSLQWQGTSGHSSIIQVMSRKGIYVVDDNDKAVTESVLIKSAPFDVSAHIALIEALMLAYVRQIVTVEKVVQTVRRFAAFNASSELPSSVEDALLFWINKVCVAVQLSVNKEDNQVLEGETNQKVRIVSKSGMSMRESVNIPLIEDLSQDIGDGCSLAVLISFYCPSALSQQDICLKSNVGIADSLYNLRLVKEFCDNYIPGKAFHLTYEDFLYMHNNLRQNVEVFLAELFYWFEIQKLDCVYSEPGSRPTTAKKMTSSAITNLPPISNVTKQSFHKYSADDGAVPRGVSQMPPRQPLLYKRQQEPDVSQQGPGVGRSRPMSAVVGKEKNDVHQSVLAWQENSQQTVQQSGGSNLLANVSIDSELMESFSDDQLGSLDMDLSDTPRPTLPANQVPRESASNLRDPNHPDYMEVQSVTSGAATNRTVHQGEETRPDSESAIPENPPCHQDLLNEFSAQLDPKDNKQSRNECYEPLLPAKLKPRKEAINNHSKEQERGDKSLLAKSVKSMEVDLGSSSVLSERSDSSSSVPVTPLPEDQLSDQDVPKQPRYTAFTVNTGFSEDMESPRKSVLSTIRSQGDTAESVKLSYTVSNEATSESARAAGIPVVGESGEQAWRRWGSREGSVNSRSSGDNSDHESQKIHYDQKLSENKDKIREVSPPKTFTRFGSKDSDVFQKPKMIVRSKTAVSPKKEPSKHMTTNFAEIKRMKNVQGNVDNSGMVYMQHGHEVNNKAMQSSTTQINNRNNQNSTSPSRTTWQQASTLKSPDQATSDSSESSDSSRPVLSELTEIRLKLEEKRKQIEKKKNRMELQQQKQRQRLGKTAFLHVLTKPTEEEVSDDLSSQGSEASLPASEEAASSSTSDKGPTAADGKTTEAPQSNKAFSREGIQQTIENVRKKWFQEDKDEQPVQMEIKPEEIMSKSCPAKNGEVLQKQKSALPAQEQNSMSMIENISDRKLSEAQELDSPSVYSRKLEKLDHDLTDLKGEILRMSLEQEQYNGSPAATSMTHPSTATRPTYAVETPERKFQGKPFHGSDLASVNQANVNTPERMQYGTPAVCRHAGYAGPIDPSVPTPERVVMGGGMSQYPQGLPPQMPITQPFQPQVMPHSLPYNGQSQFSPMPQTQVPYSGFQTVGTTPYPQPQFPPTPPHGYQQSPPFNPNLVSPHGQYGMPGVYNMTPQQYPPHSHNTSTVSAHTPVSYMYSPNHTGAQVFQPSADINKMPPASRAEMPPTSSAFNDGNITKEDTSAVSNMGVHDLSHNKSEIQSDISSDQNGFFVSFGDNSPKVKPKLSSATSQKTVTSEPTSLQNQTQQSPQQNQSSQASNLNVLAVDQNTSASSQPDNTSLLDTSGVGFVVGEDPETISKSVEEELNRKKEKFIQMQIKRKEEQDKKRQYKELEMSRRKEQERIKQEEAEKRKASEKARRDEIFRQYQDKKHQEEDNYAPPVRRNRSKTREKTRPKSMFDKGESGSVEAGVGPRKPPSLAPRPGNIRKAVSCNTLSQSTEGKLTYRRQPSPDLGRLKRGRNSAESSESGSDYAGPKLFVKPSSKSNRHIIINAISQCVLAGPVNSDQKNKVLEVLAKSDAKHFLILFRDHSCQFRGLYSFDPDSEDSIKIHGIGPKQITSKMIERFYKYNSGGKSFSEVTSTKHLSVSIDAVVIHNALWKTSAKPPQKR

Target ORF information:

RefSeq Version	XM_011449423.2
Organism	Crassostrea gigas(Pacific oyster)
Definition	Crassostrea gigas calmodulin-regulated spectrin-associated protein 1 (LOC105342461), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011449423.2

ORF Insert Sequence:

ATGATAAAAT CATCAAACAT GAATCAGTCT GTGGAAAGTG CCCCTCCTGT TGATGACATC 
ATCGAAATCA TACCCATGGA CGAGTATGAT AGCAACAAGG CAAAACTTCA GTGCTCTGTA 
TCATGGATTC TCGACAAGGC CTACAGAGAT CAAATACCAA AGGAATTCAA AGACCCATTT 
TACGAGACAC AGGATGGTGT GATGCATATT AAGCCCAAGC TGATGAATCT GATGGTTTCC 
AGTGAGCTTT ACTGTCAGGC CTGCAATAAT ATGTTCGGGG ATTCATCATT ACAATGGCAA 
GGGACATCAG GTCATTCCAG CATCATTCAA GTGATGTCCA GAAAAGGGAT ATATGTTGTT 
GATGATAACG ATAAAGCTGT TACAGAGTCT GTACTTATAA AGTCGGCTCC CTTTGATGTG 
AGTGCACACA TTGCCCTGAT CGAAGCCTTG ATGCTGGCCT ACGTTAGACA GATAGTGACG 
GTGGAGAAAG TGGTACAAAC CGTCCGAAGG TTCGCTGCCT TCAATGCCTC CAGTGAGCTG 
CCGTCCAGTG TGGAGGATGC TCTCCTGTTC TGGATAAACA AAGTCTGTGT GGCCGTACAG 
CTCAGTGTGA ACAAGGAGGA TAACCAGGTG CTGGAAGGGG AGACAAATCA GAAGGTTCGT 
ATTGTGAGTA AGTCTGGGAT GAGCATGAGG GAGTCTGTGA ATATTCCACT GATAGAGGAC 
CTCTCTCAGG ACATTGGTGA TGGCTGTTCT CTGGCTGTTC TAATCAGCTT CTACTGCCCC 
TCCGCCCTCA GTCAGCAAGA TATCTGTTTA AAGTCTAATG TAGGCATAGC TGACTCGCTA 
TATAACCTTC GCCTAGTGAA GGAATTCTGT GATAACTACA TCCCTGGCAA AGCCTTCCAT 
CTGACCTACG AAGACTTCCT GTACATGCAC AACAACCTGC GACAGAACGT GGAGGTGTTT 
CTCGCAGAGC TGTTCTACTG GTTTGAGATT CAGAAGCTGG ACTGTGTGTA TAGTGAGCCA 
GGTTCGCGGC CCACAACAGC CAAGAAAATG ACCAGCAGCG CCATCACAAA TCTTCCTCCG 
ATCAGTAACG TCACCAAGCA GAGTTTCCAC AAGTACTCTG CGGATGATGG GGCGGTTCCT 
CGCGGAGTGA GCCAGATGCC ACCAAGACAA CCCCTCCTGT ATAAACGGCA GCAGGAACCT 
GATGTCAGTC AGCAAGGTCC AGGTGTGGGA CGATCAAGAC CAATGTCTGC TGTGGTCGGG 
AAGGAGAAGA ATGATGTACA TCAGTCAGTG CTGGCCTGGC AGGAGAACAG TCAACAGACT 
GTTCAGCAGA GTGGTGGTAG TAACCTCCTG GCCAATGTCA GCATTGACTC GGAGTTGATG 
GAGAGCTTTT CTGATGATCA GCTGGGAAGT CTCGACATGG ATCTGAGTGA CACCCCCCGG 
CCCACTCTGC CAGCCAATCA AGTGCCAAGA GAATCAGCAT CAAATCTACG TGATCCAAAT 
CATCCGGATT ATATGGAGGT ACAAAGTGTG ACATCAGGAG CTGCCACAAA TAGAACTGTT 
CATCAAGGGG AGGAAACTAG ACCTGATAGT GAGTCTGCTA TACCTGAGAA TCCTCCTTGT 
CATCAAGACT TGCTAAATGA ATTCAGTGCT CAATTAGACC CTAAAGACAA TAAGCAGAGT 
AGAAATGAAT GCTATGAGCC TTTACTACCT GCAAAATTAA AACCTCGGAA AGAAGCAATA 
AATAACCACA GCAAGGAACA AGAGAGGGGA GATAAGTCTT TACTTGCTAA ATCAGTGAAA 
TCTATGGAAG TGGATCTGGG TTCTTCAAGT GTTTTAAGTG AGCGAAGTGA CAGCAGTAGT 
TCTGTTCCCG TCACTCCTCT ACCCGAGGAT CAGTTATCGG ACCAAGATGT TCCCAAACAG 
CCCCGTTATA CAGCATTTAC AGTCAACACA GGCTTTTCAG AAGACATGGA ATCTCCACGA 
AAAAGTGTAC TGTCCACCAT CCGTAGCCAA GGCGATACTG CTGAATCAGT TAAACTGAGT 
TATACTGTCT CCAATGAGGC CACTTCAGAA TCGGCGCGGG CAGCCGGGAT ACCTGTTGTC 
GGGGAGAGTG GCGAACAAGC ATGGAGGAGA TGGGGCTCCA GAGAGGGTAG TGTGAACAGT 
AGGAGTTCTG GGGACAATTC CGATCATGAA TCTCAGAAAA TTCACTACGA CCAGAAACTG 
AGTGAGAACA AAGACAAAAT CCGAGAGGTG TCACCTCCAA AAACTTTTAC TAGATTCGGT 
TCCAAGGACA GTGATGTATT TCAAAAACCG AAAATGATAG TTAGAAGTAA GACAGCTGTT 
TCACCAAAGA AAGAACCCTC CAAACATATG ACAACAAATT TTGCTGAAAT AAAGAGAATG 
AAGAATGTTC AAGGGAATGT TGACAATTCT GGAATGGTTT ACATGCAGCA TGGCCATGAA 
GTGAATAACA AAGCTATGCA AAGCTCAACA ACTCAAATCA ACAATAGGAA CAACCAAAAC 
TCAACGTCAC CTAGCAGAAC AACATGGCAG CAAGCAAGCA CTCTAAAATC TCCTGATCAG 
GCCACAAGTG ATTCCTCAGA AAGCAGTGAC AGTAGTCGCC CCGTGCTGTC TGAGCTAACA 
GAAATCCGGC TTAAGTTGGA GGAAAAGCGT AAACAGATAG AGAAAAAGAA GAACAGAATG 
GAGCTACAGC AACAGAAACA GAGACAGCGG CTAGGGAAGA CAGCTTTTCT TCATGTGCTG 
ACAAAACCCA CTGAGGAGGA GGTGTCTGAT GACTTGAGTT CACAGGGAAG TGAGGCGTCT 
CTACCAGCAA GTGAGGAGGC TGCCTCAAGT TCAACCTCGG ACAAAGGACC AACAGCAGCA 
GATGGAAAAA CTACAGAAGC ACCTCAAAGC AACAAAGCAT TTTCTAGGGA GGGGATACAG 
CAAACAATAG AAAATGTTAG AAAGAAGTGG TTTCAAGAGG ACAAGGATGA GCAACCTGTG 
CAGATGGAGA TAAAACCAGA AGAAATAATG AGTAAGAGTT GTCCAGCCAA AAATGGAGAA 
GTTTTACAAA AACAAAAATC GGCGCTGCCT GCCCAGGAGC AAAATTCAAT GTCAATGATA 
GAAAATATAT CTGACAGAAA ATTATCTGAA GCTCAAGAAT TGGACAGTCC ATCTGTATAC 
AGTCGGAAGC TTGAAAAACT GGATCATGAC CTGACTGATT TGAAAGGGGA GATCCTGAGG 
ATGTCTCTGG AACAGGAGCA GTACAATGGG AGTCCAGCGG CTACTTCCAT GACACACCCA 
TCCACAGCCA CCAGGCCAAC TTATGCAGTG GAAACTCCAG AACGGAAATT CCAGGGAAAA 
CCATTCCATG GATCAGATCT CGCCTCAGTG AATCAGGCAA ACGTAAACAC TCCAGAGAGA 
ATGCAGTATG GAACCCCGGC GGTTTGTCGC CATGCAGGAT ATGCAGGCCC CATTGATCCC 
AGTGTACCCA CCCCAGAGAG GGTTGTGATG GGGGGAGGTA TGTCTCAGTA CCCCCAGGGC 
CTACCCCCAC AGATGCCTAT TACACAACCA TTCCAGCCCC AAGTGATGCC CCACTCTTTA 
CCCTATAATG GACAGAGTCA GTTCAGTCCA ATGCCACAAA CACAGGTGCC ATACAGCGGT 
TTCCAAACTG TTGGTACTAC TCCCTATCCA CAACCCCAGT TCCCTCCCAC CCCACCCCAT 
GGCTACCAAC AGTCCCCTCC CTTCAATCCC AATCTGGTGT CACCCCACGG TCAATATGGG 
ATGCCTGGGG TATATAATAT GACTCCTCAG CAGTACCCTC CCCATTCTCA CAACACATCT 
ACAGTGTCAG CTCATACTCC TGTCTCTTAC ATGTACTCGC CCAATCACAC CGGAGCTCAA 
GTCTTTCAAC CTTCTGCGGA TATTAATAAA ATGCCGCCTG CTTCTCGGGC TGAAATGCCC 
CCCACTTCTA GTGCTTTCAA TGATGGTAAC ATCACCAAAG AAGACACCTC TGCTGTTTCA 
AATATGGGTG TTCATGACTT GTCTCATAAC AAAAGTGAAA TACAAAGTGA CATTTCTAGT 
GACCAGAATG GATTTTTTGT GTCATTTGGA GACAATTCTC CAAAGGTTAA ACCCAAACTT 
TCTAGTGCAA CATCTCAAAA GACGGTAACC TCTGAACCAA CTAGTCTCCA AAACCAAACA 
CAACAGTCAC CTCAGCAGAA CCAGTCTAGT CAAGCCAGCA ACCTGAATGT GTTGGCCGTA 
GATCAGAACA CGTCGGCAAG CTCCCAGCCC GACAACACAT CCCTGCTTGA TACCTCGGGG 
GTCGGGTTTG TGGTGGGAGA AGACCCAGAA ACTATCTCAA AGTCAGTGGA AGAAGAATTG 
AACAGAAAAA AAGAAAAGTT TATACAAATG CAGATCAAAC GCAAAGAAGA ACAAGACAAA 
AAACGTCAAT ATAAAGAGTT GGAAATGTCA CGCAGGAAAG AGCAAGAGAG AATTAAACAA 
GAAGAGGCAG AAAAACGTAA AGCCAGTGAA AAGGCACGAA GAGATGAAAT ATTCCGTCAG 
TACCAGGATA AGAAGCATCA AGAGGAAGAC AACTACGCAC CTCCTGTCCG CAGGAACCGA 
TCCAAAACCC GGGAAAAGAC CCGCCCCAAA TCCATGTTCG ACAAAGGAGA ATCAGGATCA 
GTGGAAGCGG GTGTTGGTCC TAGAAAACCC CCAAGCTTAG CACCACGCCC AGGAAACATT 
CGAAAAGCAG TGTCCTGTAA CACCCTGTCC CAATCCACGG AGGGCAAGCT GACCTACAGG 
AGACAGCCTT CTCCAGATCT TGGTCGTTTG AAGAGAGGCA GGAACAGTGC AGAGAGCAGT 
GAATCTGGAT CAGATTATGC AGGACCCAAG TTGTTTGTGA AGCCAAGCTC CAAGTCAAAC 
CGCCACATCA TCATCAACGC CATTAGTCAG TGTGTGCTAG CTGGACCCGT GAACTCCGAT 
CAGAAAAACA AAGTATTGGA GGTTTTGGCT AAATCAGATG CCAAGCACTT CCTGATATTG 
TTCCGAGACC ACTCCTGTCA GTTCCGGGGA CTGTACAGCT TTGATCCAGA TTCAGAGGAC 
TCCATTAAAA TCCATGGAAT AGGACCCAAA CAAATCACCA GCAAAATGAT CGAAAGATTT 
TACAAGTACA ATTCTGGTGG AAAAAGCTTT AGTGAGGTTA CATCTACGAA ACATTTATCA 
GTGTCAATAG ACGCAGTAGT TATACACAAT GCCTTGTGGA AAACATCAGC AAAGCCGCCA 
CAAAAAAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC105342461 cDNA ORF clone, Crassostrea gigas(Pacific oyster)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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