Fh5 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fh5
Entrez Gene ID	835554
Full Name	formin homology5
Synonyms	ATFH5,FORMIN HOMOLOGY 2 DOMAIN-CONTAINING PROTEIN 5,FORMIN HOMOLOGY 5,MRB17.15,MRB17_15,formin homology5
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_124846.2	NP_200276.1	formin homology5
NM_180860.2	NP_851191.1	formin homology5

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

show that a tip-located, the Arabidopsis formin homology5 (FH5), stimulates actin assembly from the subapical membrane, provides actin filaments for vesicular trafficking to the apical dome, and mediates assembly of the subapical actin structure
Title: A transmembrane formin nucleates subapical actin assembly and controls tip-focused growth in pollen tubes.

AtFH5 is responsible for morphological defects caused by the loss of PcG activity in the seed.
Title: Polycomb group-dependent imprinting of the actin regulator AtFH5 regulates morphogenesis in Arabidopsis thaliana.

The following Fh5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fh5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb21426	NM_124846.2 Latest version!	Arabidopsis thaliana formin homology5 (Fh5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb21426	NM_180860.2 Latest version!	Arabidopsis thaliana formin homology5 (Fh5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fh5 ( NM_124846.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_200276.1 Arabidopsis thaliana formin homology5 (Fh5), mRNA.

Price & Availability↑

CloneID	OAb21426
Clone ID Related Accession (Same CDS sequence)	NM_124846.2 , NM_180860.2
Accession Version	NM_124846.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	formin homology5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On May 13, 2003 this sequence version replaced NM_124846.1.

ATGGTTGGAA TGATTCGAGG AGGAATGGGT GATCAGAATT GGAGTCGTTT AGTTTTCTGG 
TTGATTCTGT TTTCTGGGTT ATTGGTAATA ACTTTGGAAG AGAATCCAGA AAAAGATGAG 
ATTTTTCTTA GTCAATTCAT GGCCCCATCA ACAGGGCAGG TCAATGAACA TATGGAAGAA 
ACATCATGGG CGCAGCGTTG TTGGCAAGAT TCAGATTGTG TGAAGGAAGC TGTGGCAGAA 
TTCAATTTAT GTTTTCCAGG ATCAAAGGAT AGTAGGGAAC TATTTGGTTT GAACCATACC 
AATCTGAAGC AGACTCTTCT TGATTGTATC CAAGAAAAAG GAAAGTTAAA TGGTCATAAC 
CCCAAGTATC TGGAGCTCTT ATCATCTATG CTTGATATTC CGAGGAGAAA TCTAGCTACT 
AAACCCGGCT CTTCCCCTTC CCCTTCACCT TCCCGCCCTC CTAAGCGTAG TCGGGGGCCT 
CCGCGCCCAC CTACTCGACC AAAATCCCCA CCTCCTCGGA AATCATCTTT TCCACCATCA 
AGATCTCCCC CTCCTCCTCC TGCTAAGAAA AACGCTTCTA AAAATTCAAC TTCTGCACCT 
GTTTCACCTG CGAAAAAAAA AGAGGATCAT GAGAAAACAA TCATCATTGC TGTTGTTGTG 
ACTGCAGTTT CTACGTTTTT GTTGGCTGCA TTATTCTTCT TATGCTGCTC CAGGGTTTGT 
GGAAATGGTT CAGGAGGCAG GAAAAACGAT GAAAGGCCTC TTCTCAGTTT AAGCAGTAGT 
GATTACTCTG TTGGTTCCTC CATCAACTAT GGTGGTTCGG TCAAAGGAGA TAAGCAGGGT 
CATCAATCTT TTAATATTTA CTCAAACCAA GGAAAAATGT CATCTTTCGA TGGAAGTAAC 
TCTGACACTT CAGATTCGTT AGAAGAACGG CTATCACACG AAGGCCTGAG AAATAATAGT 
ATTACCAATC ACGGGCTTCC ACCTTTGAAG CCTCCACCTG GAAGAACAGC TTCTGTACTT 
TCAGGGAAGT CTTTTTCTGG AAAAGTGGAG CCTCTTCCAC CGGAACCACC CAAATTTCTT 
AAGGTATCCT CGAAAAAGGC TTCTGCTCCT CCACCGCCAG TTCCAGCACC GCAAATGCCA 
TCTTCTGCTG GTCCTCCACG ACCGCCCCCT CCAGCTCCGC CACCTGGTTC TGGTGGGCCT 
AAGCCACCAC CTCCTCCTGG ACCAAAAGGA CCACGTCCGC CTCCACCAAT GAGTCTGGGC 
CCAAAAGCAC CCCGACCGCC TTCTGGGCCA GCAGATGCAT TAGATGATGA TGCTCCCAAA 
ACAAAGCTGA AGCCATTTTT CTGGGATAAA GTGCAAGCAA ACCCAGAACA TTCAATGGTT 
TGGAATGATA TAAGATCAGG ATCCTTTCAG TTCAATGAAG AGATGATAGA GTCGTTATTT 
GGATATGCAG CAGCAGACAA GAATAAAAAC GACAAGAAGG GGTCCTCTGG ACAAGCTGCT 
TTACCTCAGT TTGTTCAGAT CCTCGAACCA AAGAAAGGAC AAAATCTATC AATTCTTTTG 
CGTGCTTTGA ATGCGACAAC TGAAGAAGTG TGTGATGCAC TTCGCGAAGG AAATGAGCTT 
CCTGTAGAAT TCATTCAGAC TCTGTTAAAG ATGGCACCAA CACCAGAAGA AGAACTAAAA 
CTTAGATTAT ACTGCGGTGA AATAGCTCAA CTGGGATCCG CTGAACGATT CCTGAAAGCA 
GTCGTTGATA TCCCTTTTGC TTTCAAGCGT CTAGAAGCAC TTCTATTTAT GTGTACACTC 
CATGAAGAAA TGGCTTTTGT TAAAGAATCA TTTCAGAAAT TGGAGGTTGC TTGTAAAGAA 
CTTAGGGGAA GTCGGCTGTT CCTGAAGCTC TTAGAGGCAG TTCTTAAGAC CGGAAACCGA 
ATGAATGATG GTACATTTAG GGGTGGTGCT CAAGCTTTCA AGCTGGACAC TCTTTTGAAA 
TTAGCAGACG TCAAGGGAAC AGATGGGAAA ACAACGCTCC TCCACTTTGT TGTTCAAGAG 
ATAATTAGGA CTGAAGGTGT AAGAGCGGCT CGTACTATCA GAGAGAGCCA AAGTTTCTCA 
AGTGTTAAAA CAGAGGATCT CCTAGTCGAG GAAACCTCTG AAGAGTCAGA AGAGAATTAC 
CGTAATCTTG GACTTGAGAA AGTTTCAGGC TTGAGCAGTG AGCTTGAACA TGTAAAGAAA 
TCTGCAAACA TAGATGCCGA TGGTTTAACA GGGACTGTTC TTAAAATGGG TCATGCTTTG 
TCGAAAGCTC GAGACTTTGT CAACTCAGAG ATGAAAAGTT CAGGCGAGGA GAGTGGGTTT 
CGTGAAGCGC TTGAAGATTT TATTCAAAAT GCTGAAGGAA GCATCATGTC CATACTCGAG 
GAAGAGAAGA GAATAATGGC TTTGGTGAAA AGTACAGGGG ACTATTTCCA TGGCAAAGCA 
GGAAAAGACG AAGGGTTGCG TTTGTTTGTG ATTGTGCGCG ATTTTCTGAT AATCTTAGAT 
AAATCCTGCA AAGAAGTCAG AGAAGCACGA GGAAGACCCG TAAGGATGGC CAGAAAACAA 
GGCTCAACAG CCTCAGCATC TTCTGAAACT CCAAGACAAA CGCCTTCATT AGATCCTAGG 
CAGAAGCTGT TTCCAGCTAT TACAGAGAGA CGCGTGGATC AGTCTAGTTC AGATTCAGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_200276.1
CDS	236..2938
Translation	MVGMIRGGMGDQNWSRLVFWLILFSGLLVITLEENPEKDEIFLSQFMAPSTGQVNEHMEETSWAQRCWQDSDCVKEAVAEFNLCFPGSKDSRELFGLNHTNLKQTLLDCIQEKGKLNGHNPKYLELLSSMLDIPRRNLATKPGSSPSPSPSRPPKRSRGPPRPPTRPKSPPPRKSSFPPSRSPPPPPAKKNASKNSTSAPVSPAKKKEDHEKTIIIAVVVTAVSTFLLAALFFLCCSRVCGNGSGGRKNDERPLLSLSSSDYSVGSSINYGGSVKGDKQGHQSFNIYSNQGKMSSFDGSNSDTSDSLEERLSHEGLRNNSITNHGLPPLKPPPGRTASVLSGKSFSGKVEPLPPEPPKFLKVSSKKASAPPPPVPAPQMPSSAGPPRPPPPAPPPGSGGPKPPPPPGPKGPRPPPPMSLGPKAPRPPSGPADALDDDAPKTKLKPFFWDKVQANPEHSMVWNDIRSGSFQFNEEMIESLFGYAAADKNKNDKKGSSGQAALPQFVQILEPKKGQNLSILLRALNATTEEVCDALREGNELPVEFIQTLLKMAPTPEEELKLRLYCGEIAQLGSAERFLKAVVDIPFAFKRLEALLFMCTLHEEMAFVKESFQKLEVACKELRGSRLFLKLLEAVLKTGNRMNDGTFRGGAQAFKLDTLLKLADVKGTDGKTTLLHFVVQEIIRTEGVRAARTIRESQSFSSVKTEDLLVEETSEESEENYRNLGLEKVSGLSSELEHVKKSANIDADGLTGTVLKMGHALSKARDFVNSEMKSSGEESGFREALEDFIQNAEGSIMSILEEEKRIMALVKSTGDYFHGKAGKDEGLRLFVIVRDFLIILDKSCKEVREARGRPVRMARKQGSTASASSETPRQTPSLDPRQKLFPAITERRVDQSSSDSD

Target ORF information:

RefSeq Version	NM_124846.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana formin homology5 (Fh5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_124846.2

ORF Insert Sequence:

ATGGTTGGAA TGATTCGAGG AGGAATGGGT GATCAGAATT GGAGTCGTTT AGTTTTCTGG 
TTGATTCTGT TTTCTGGGTT ATTGGTAATA ACTTTGGAAG AGAATCCAGA AAAAGATGAG 
ATTTTTCTTA GTCAATTCAT GGCCCCATCA ACAGGGCAGG TCAATGAACA TATGGAAGAA 
ACATCATGGG CGCAGCGTTG TTGGCAAGAT TCAGATTGTG TGAAGGAAGC TGTGGCAGAA 
TTCAATTTAT GTTTTCCAGG ATCAAAGGAT AGTAGGGAAC TATTTGGTTT GAACCATACC 
AATCTGAAGC AGACTCTTCT TGATTGTATC CAAGAAAAAG GAAAGTTAAA TGGTCATAAC 
CCCAAGTATC TGGAGCTCTT ATCATCTATG CTTGATATTC CGAGGAGAAA TCTAGCTACT 
AAACCCGGCT CTTCCCCTTC CCCTTCACCT TCCCGCCCTC CTAAGCGTAG TCGGGGGCCT 
CCGCGCCCAC CTACTCGACC AAAATCCCCA CCTCCTCGGA AATCATCTTT TCCACCATCA 
AGATCTCCCC CTCCTCCTCC TGCTAAGAAA AACGCTTCTA AAAATTCAAC TTCTGCACCT 
GTTTCACCTG CGAAAAAAAA AGAGGATCAT GAGAAAACAA TCATCATTGC TGTTGTTGTG 
ACTGCAGTTT CTACGTTTTT GTTGGCTGCA TTATTCTTCT TATGCTGCTC CAGGGTTTGT 
GGAAATGGTT CAGGAGGCAG GAAAAACGAT GAAAGGCCTC TTCTCAGTTT AAGCAGTAGT 
GATTACTCTG TTGGTTCCTC CATCAACTAT GGTGGTTCGG TCAAAGGAGA TAAGCAGGGT 
CATCAATCTT TTAATATTTA CTCAAACCAA GGAAAAATGT CATCTTTCGA TGGAAGTAAC 
TCTGACACTT CAGATTCGTT AGAAGAACGG CTATCACACG AAGGCCTGAG AAATAATAGT 
ATTACCAATC ACGGGCTTCC ACCTTTGAAG CCTCCACCTG GAAGAACAGC TTCTGTACTT 
TCAGGGAAGT CTTTTTCTGG AAAAGTGGAG CCTCTTCCAC CGGAACCACC CAAATTTCTT 
AAGGTATCCT CGAAAAAGGC TTCTGCTCCT CCACCGCCAG TTCCAGCACC GCAAATGCCA 
TCTTCTGCTG GTCCTCCACG ACCGCCCCCT CCAGCTCCGC CACCTGGTTC TGGTGGGCCT 
AAGCCACCAC CTCCTCCTGG ACCAAAAGGA CCACGTCCGC CTCCACCAAT GAGTCTGGGC 
CCAAAAGCAC CCCGACCGCC TTCTGGGCCA GCAGATGCAT TAGATGATGA TGCTCCCAAA 
ACAAAGCTGA AGCCATTTTT CTGGGATAAA GTGCAAGCAA ACCCAGAACA TTCAATGGTT 
TGGAATGATA TAAGATCAGG ATCCTTTCAG TTCAATGAAG AGATGATAGA GTCGTTATTT 
GGATATGCAG CAGCAGACAA GAATAAAAAC GACAAGAAGG GGTCCTCTGG ACAAGCTGCT 
TTACCTCAGT TTGTTCAGAT CCTCGAACCA AAGAAAGGAC AAAATCTATC AATTCTTTTG 
CGTGCTTTGA ATGCGACAAC TGAAGAAGTG TGTGATGCAC TTCGCGAAGG AAATGAGCTT 
CCTGTAGAAT TCATTCAGAC TCTGTTAAAG ATGGCACCAA CACCAGAAGA AGAACTAAAA 
CTTAGATTAT ACTGCGGTGA AATAGCTCAA CTGGGATCCG CTGAACGATT CCTGAAAGCA 
GTCGTTGATA TCCCTTTTGC TTTCAAGCGT CTAGAAGCAC TTCTATTTAT GTGTACACTC 
CATGAAGAAA TGGCTTTTGT TAAAGAATCA TTTCAGAAAT TGGAGGTTGC TTGTAAAGAA 
CTTAGGGGAA GTCGGCTGTT CCTGAAGCTC TTAGAGGCAG TTCTTAAGAC CGGAAACCGA 
ATGAATGATG GTACATTTAG GGGTGGTGCT CAAGCTTTCA AGCTGGACAC TCTTTTGAAA 
TTAGCAGACG TCAAGGGAAC AGATGGGAAA ACAACGCTCC TCCACTTTGT TGTTCAAGAG 
ATAATTAGGA CTGAAGGTGT AAGAGCGGCT CGTACTATCA GAGAGAGCCA AAGTTTCTCA 
AGTGTTAAAA CAGAGGATCT CCTAGTCGAG GAAACCTCTG AAGAGTCAGA AGAGAATTAC 
CGTAATCTTG GACTTGAGAA AGTTTCAGGC TTGAGCAGTG AGCTTGAACA TGTAAAGAAA 
TCTGCAAACA TAGATGCCGA TGGTTTAACA GGGACTGTTC TTAAAATGGG TCATGCTTTG 
TCGAAAGCTC GAGACTTTGT CAACTCAGAG ATGAAAAGTT CAGGCGAGGA GAGTGGGTTT 
CGTGAAGCGC TTGAAGATTT TATTCAAAAT GCTGAAGGAA GCATCATGTC CATACTCGAG 
GAAGAGAAGA GAATAATGGC TTTGGTGAAA AGTACAGGGG ACTATTTCCA TGGCAAAGCA 
GGAAAAGACG AAGGGTTGCG TTTGTTTGTG ATTGTGCGCG ATTTTCTGAT AATCTTAGAT 
AAATCCTGCA AAGAAGTCAG AGAAGCACGA GGAAGACCCG TAAGGATGGC CAGAAAACAA 
GGCTCAACAG CCTCAGCATC TTCTGAAACT CCAAGACAAA CGCCTTCATT AGATCCTAGG 
CAGAAGCTGT TTCCAGCTAT TACAGAGAGA CGCGTGGATC AGTCTAGTTC AGATTCAGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fh5 ( NM_180860.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_851191.1 Arabidopsis thaliana formin homology5 (Fh5), mRNA.

Price & Availability↑

CloneID	OAb21426
Clone ID Related Accession (Same CDS sequence)	NM_124846.2 , NM_180860.2
Accession Version	NM_180860.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	formin homology5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_180860.1.

ATGGTTGGAA TGATTCGAGG AGGAATGGGT GATCAGAATT GGAGTCGTTT AGTTTTCTGG 
TTGATTCTGT TTTCTGGGTT ATTGGTAATA ACTTTGGAAG AGAATCCAGA AAAAGATGAG 
ATTTTTCTTA GTCAATTCAT GGCCCCATCA ACAGGGCAGG TCAATGAACA TATGGAAGAA 
ACATCATGGG CGCAGCGTTG TTGGCAAGAT TCAGATTGTG TGAAGGAAGC TGTGGCAGAA 
TTCAATTTAT GTTTTCCAGG ATCAAAGGAT AGTAGGGAAC TATTTGGTTT GAACCATACC 
AATCTGAAGC AGACTCTTCT TGATTGTATC CAAGAAAAAG GAAAGTTAAA TGGTCATAAC 
CCCAAGTATC TGGAGCTCTT ATCATCTATG CTTGATATTC CGAGGAGAAA TCTAGCTACT 
AAACCCGGCT CTTCCCCTTC CCCTTCACCT TCCCGCCCTC CTAAGCGTAG TCGGGGGCCT 
CCGCGCCCAC CTACTCGACC AAAATCCCCA CCTCCTCGGA AATCATCTTT TCCACCATCA 
AGATCTCCCC CTCCTCCTCC TGCTAAGAAA AACGCTTCTA AAAATTCAAC TTCTGCACCT 
GTTTCACCTG CGAAAAAAAA AGAGGATCAT GAGAAAACAA TCATCATTGC TGTTGTTGTG 
ACTGCAGTTT CTACGTTTTT GTTGGCTGCA TTATTCTTCT TATGCTGCTC CAGGGTTTGT 
GGAAATGGTT CAGGAGGCAG GAAAAACGAT GAAAGGCCTC TTCTCAGTTT AAGCAGTAGT 
GATTACTCTG TTGGTTCCTC CATCAACTAT GGTGGTTCGG TCAAAGGAGA TAAGCAGGGT 
CATCAATCTT TTAATATTTA CTCAAACCAA GGAAAAATGT CATCTTTCGA TGGAAGTAAC 
TCTGACACTT CAGATTCGTT AGAAGAACGG CTATCACACG AAGGCCTGAG AAATAATAGT 
ATTACCAATC ACGGGCTTCC ACCTTTGAAG CCTCCACCTG GAAGAACAGC TTCTGTACTT 
TCAGGGAAGT CTTTTTCTGG AAAAGTGGAG CCTCTTCCAC CGGAACCACC CAAATTTCTT 
AAGGTATCCT CGAAAAAGGC TTCTGCTCCT CCACCGCCAG TTCCAGCACC GCAAATGCCA 
TCTTCTGCTG GTCCTCCACG ACCGCCCCCT CCAGCTCCGC CACCTGGTTC TGGTGGGCCT 
AAGCCACCAC CTCCTCCTGG ACCAAAAGGA CCACGTCCGC CTCCACCAAT GAGTCTGGGC 
CCAAAAGCAC CCCGACCGCC TTCTGGGCCA GCAGATGCAT TAGATGATGA TGCTCCCAAA 
ACAAAGCTGA AGCCATTTTT CTGGGATAAA GTGCAAGCAA ACCCAGAACA TTCAATGGTT 
TGGAATGATA TAAGATCAGG ATCCTTTCAG TTCAATGAAG AGATGATAGA GTCGTTATTT 
GGATATGCAG CAGCAGACAA GAATAAAAAC GACAAGAAGG GGTCCTCTGG ACAAGCTGCT 
TTACCTCAGT TTGTTCAGAT CCTCGAACCA AAGAAAGGAC AAAATCTATC AATTCTTTTG 
CGTGCTTTGA ATGCGACAAC TGAAGAAGTG TGTGATGCAC TTCGCGAAGG AAATGAGCTT 
CCTGTAGAAT TCATTCAGAC TCTGTTAAAG ATGGCACCAA CACCAGAAGA AGAACTAAAA 
CTTAGATTAT ACTGCGGTGA AATAGCTCAA CTGGGATCCG CTGAACGATT CCTGAAAGCA 
GTCGTTGATA TCCCTTTTGC TTTCAAGCGT CTAGAAGCAC TTCTATTTAT GTGTACACTC 
CATGAAGAAA TGGCTTTTGT TAAAGAATCA TTTCAGAAAT TGGAGGTTGC TTGTAAAGAA 
CTTAGGGGAA GTCGGCTGTT CCTGAAGCTC TTAGAGGCAG TTCTTAAGAC CGGAAACCGA 
ATGAATGATG GTACATTTAG GGGTGGTGCT CAAGCTTTCA AGCTGGACAC TCTTTTGAAA 
TTAGCAGACG TCAAGGGAAC AGATGGGAAA ACAACGCTCC TCCACTTTGT TGTTCAAGAG 
ATAATTAGGA CTGAAGGTGT AAGAGCGGCT CGTACTATCA GAGAGAGCCA AAGTTTCTCA 
AGTGTTAAAA CAGAGGATCT CCTAGTCGAG GAAACCTCTG AAGAGTCAGA AGAGAATTAC 
CGTAATCTTG GACTTGAGAA AGTTTCAGGC TTGAGCAGTG AGCTTGAACA TGTAAAGAAA 
TCTGCAAACA TAGATGCCGA TGGTTTAACA GGGACTGTTC TTAAAATGGG TCATGCTTTG 
TCGAAAGCTC GAGACTTTGT CAACTCAGAG ATGAAAAGTT CAGGCGAGGA GAGTGGGTTT 
CGTGAAGCGC TTGAAGATTT TATTCAAAAT GCTGAAGGAA GCATCATGTC CATACTCGAG 
GAAGAGAAGA GAATAATGGC TTTGGTGAAA AGTACAGGGG ACTATTTCCA TGGCAAAGCA 
GGAAAAGACG AAGGGTTGCG TTTGTTTGTG ATTGTGCGCG ATTTTCTGAT AATCTTAGAT 
AAATCCTGCA AAGAAGTCAG AGAAGCACGA GGAAGACCCG TAAGGATGGC CAGAAAACAA 
GGCTCAACAG CCTCAGCATC TTCTGAAACT CCAAGACAAA CGCCTTCATT AGATCCTAGG 
CAGAAGCTGT TTCCAGCTAT TACAGAGAGA CGCGTGGATC AGTCTAGTTC AGATTCAGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851191.1
CDS	479..3181
Translation	MVGMIRGGMGDQNWSRLVFWLILFSGLLVITLEENPEKDEIFLSQFMAPSTGQVNEHMEETSWAQRCWQDSDCVKEAVAEFNLCFPGSKDSRELFGLNHTNLKQTLLDCIQEKGKLNGHNPKYLELLSSMLDIPRRNLATKPGSSPSPSPSRPPKRSRGPPRPPTRPKSPPPRKSSFPPSRSPPPPPAKKNASKNSTSAPVSPAKKKEDHEKTIIIAVVVTAVSTFLLAALFFLCCSRVCGNGSGGRKNDERPLLSLSSSDYSVGSSINYGGSVKGDKQGHQSFNIYSNQGKMSSFDGSNSDTSDSLEERLSHEGLRNNSITNHGLPPLKPPPGRTASVLSGKSFSGKVEPLPPEPPKFLKVSSKKASAPPPPVPAPQMPSSAGPPRPPPPAPPPGSGGPKPPPPPGPKGPRPPPPMSLGPKAPRPPSGPADALDDDAPKTKLKPFFWDKVQANPEHSMVWNDIRSGSFQFNEEMIESLFGYAAADKNKNDKKGSSGQAALPQFVQILEPKKGQNLSILLRALNATTEEVCDALREGNELPVEFIQTLLKMAPTPEEELKLRLYCGEIAQLGSAERFLKAVVDIPFAFKRLEALLFMCTLHEEMAFVKESFQKLEVACKELRGSRLFLKLLEAVLKTGNRMNDGTFRGGAQAFKLDTLLKLADVKGTDGKTTLLHFVVQEIIRTEGVRAARTIRESQSFSSVKTEDLLVEETSEESEENYRNLGLEKVSGLSSELEHVKKSANIDADGLTGTVLKMGHALSKARDFVNSEMKSSGEESGFREALEDFIQNAEGSIMSILEEEKRIMALVKSTGDYFHGKAGKDEGLRLFVIVRDFLIILDKSCKEVREARGRPVRMARKQGSTASASSETPRQTPSLDPRQKLFPAITERRVDQSSSDSD

Target ORF information:

RefSeq Version	NM_180860.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana formin homology5 (Fh5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_180860.2

ORF Insert Sequence:

ATGGTTGGAA TGATTCGAGG AGGAATGGGT GATCAGAATT GGAGTCGTTT AGTTTTCTGG 
TTGATTCTGT TTTCTGGGTT ATTGGTAATA ACTTTGGAAG AGAATCCAGA AAAAGATGAG 
ATTTTTCTTA GTCAATTCAT GGCCCCATCA ACAGGGCAGG TCAATGAACA TATGGAAGAA 
ACATCATGGG CGCAGCGTTG TTGGCAAGAT TCAGATTGTG TGAAGGAAGC TGTGGCAGAA 
TTCAATTTAT GTTTTCCAGG ATCAAAGGAT AGTAGGGAAC TATTTGGTTT GAACCATACC 
AATCTGAAGC AGACTCTTCT TGATTGTATC CAAGAAAAAG GAAAGTTAAA TGGTCATAAC 
CCCAAGTATC TGGAGCTCTT ATCATCTATG CTTGATATTC CGAGGAGAAA TCTAGCTACT 
AAACCCGGCT CTTCCCCTTC CCCTTCACCT TCCCGCCCTC CTAAGCGTAG TCGGGGGCCT 
CCGCGCCCAC CTACTCGACC AAAATCCCCA CCTCCTCGGA AATCATCTTT TCCACCATCA 
AGATCTCCCC CTCCTCCTCC TGCTAAGAAA AACGCTTCTA AAAATTCAAC TTCTGCACCT 
GTTTCACCTG CGAAAAAAAA AGAGGATCAT GAGAAAACAA TCATCATTGC TGTTGTTGTG 
ACTGCAGTTT CTACGTTTTT GTTGGCTGCA TTATTCTTCT TATGCTGCTC CAGGGTTTGT 
GGAAATGGTT CAGGAGGCAG GAAAAACGAT GAAAGGCCTC TTCTCAGTTT AAGCAGTAGT 
GATTACTCTG TTGGTTCCTC CATCAACTAT GGTGGTTCGG TCAAAGGAGA TAAGCAGGGT 
CATCAATCTT TTAATATTTA CTCAAACCAA GGAAAAATGT CATCTTTCGA TGGAAGTAAC 
TCTGACACTT CAGATTCGTT AGAAGAACGG CTATCACACG AAGGCCTGAG AAATAATAGT 
ATTACCAATC ACGGGCTTCC ACCTTTGAAG CCTCCACCTG GAAGAACAGC TTCTGTACTT 
TCAGGGAAGT CTTTTTCTGG AAAAGTGGAG CCTCTTCCAC CGGAACCACC CAAATTTCTT 
AAGGTATCCT CGAAAAAGGC TTCTGCTCCT CCACCGCCAG TTCCAGCACC GCAAATGCCA 
TCTTCTGCTG GTCCTCCACG ACCGCCCCCT CCAGCTCCGC CACCTGGTTC TGGTGGGCCT 
AAGCCACCAC CTCCTCCTGG ACCAAAAGGA CCACGTCCGC CTCCACCAAT GAGTCTGGGC 
CCAAAAGCAC CCCGACCGCC TTCTGGGCCA GCAGATGCAT TAGATGATGA TGCTCCCAAA 
ACAAAGCTGA AGCCATTTTT CTGGGATAAA GTGCAAGCAA ACCCAGAACA TTCAATGGTT 
TGGAATGATA TAAGATCAGG ATCCTTTCAG TTCAATGAAG AGATGATAGA GTCGTTATTT 
GGATATGCAG CAGCAGACAA GAATAAAAAC GACAAGAAGG GGTCCTCTGG ACAAGCTGCT 
TTACCTCAGT TTGTTCAGAT CCTCGAACCA AAGAAAGGAC AAAATCTATC AATTCTTTTG 
CGTGCTTTGA ATGCGACAAC TGAAGAAGTG TGTGATGCAC TTCGCGAAGG AAATGAGCTT 
CCTGTAGAAT TCATTCAGAC TCTGTTAAAG ATGGCACCAA CACCAGAAGA AGAACTAAAA 
CTTAGATTAT ACTGCGGTGA AATAGCTCAA CTGGGATCCG CTGAACGATT CCTGAAAGCA 
GTCGTTGATA TCCCTTTTGC TTTCAAGCGT CTAGAAGCAC TTCTATTTAT GTGTACACTC 
CATGAAGAAA TGGCTTTTGT TAAAGAATCA TTTCAGAAAT TGGAGGTTGC TTGTAAAGAA 
CTTAGGGGAA GTCGGCTGTT CCTGAAGCTC TTAGAGGCAG TTCTTAAGAC CGGAAACCGA 
ATGAATGATG GTACATTTAG GGGTGGTGCT CAAGCTTTCA AGCTGGACAC TCTTTTGAAA 
TTAGCAGACG TCAAGGGAAC AGATGGGAAA ACAACGCTCC TCCACTTTGT TGTTCAAGAG 
ATAATTAGGA CTGAAGGTGT AAGAGCGGCT CGTACTATCA GAGAGAGCCA AAGTTTCTCA 
AGTGTTAAAA CAGAGGATCT CCTAGTCGAG GAAACCTCTG AAGAGTCAGA AGAGAATTAC 
CGTAATCTTG GACTTGAGAA AGTTTCAGGC TTGAGCAGTG AGCTTGAACA TGTAAAGAAA 
TCTGCAAACA TAGATGCCGA TGGTTTAACA GGGACTGTTC TTAAAATGGG TCATGCTTTG 
TCGAAAGCTC GAGACTTTGT CAACTCAGAG ATGAAAAGTT CAGGCGAGGA GAGTGGGTTT 
CGTGAAGCGC TTGAAGATTT TATTCAAAAT GCTGAAGGAA GCATCATGTC CATACTCGAG 
GAAGAGAAGA GAATAATGGC TTTGGTGAAA AGTACAGGGG ACTATTTCCA TGGCAAAGCA 
GGAAAAGACG AAGGGTTGCG TTTGTTTGTG ATTGTGCGCG ATTTTCTGAT AATCTTAGAT 
AAATCCTGCA AAGAAGTCAG AGAAGCACGA GGAAGACCCG TAAGGATGGC CAGAAAACAA 
GGCTCAACAG CCTCAGCATC TTCTGAAACT CCAAGACAAA CGCCTTCATT AGATCCTAGG 
CAGAAGCTGT TTCCAGCTAT TACAGAGAGA CGCGTGGATC AGTCTAGTTC AGATTCAGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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Fh5 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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