GHRL cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	GHRL
Entrez Gene ID	408185
Full Name	ghrelin/obestatin prepropeptide
Synonyms	ghrelin,preproghrelin
General protein information	Preferred Names ghrelin/obestatin prepropeptide Names appetite-regulating hormone ghrelin/obestatin preprohormone
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015293041.1	XP_015148527.1	appetite-regulating hormone isoform X1
NM_001001131.1	NP_001001131.1	appetite-regulating hormone preproprotein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Is really endogenous ghrelin a hunger signal in chickens? Association of GHSR SNPs with increase appetite, growth traits, expression and serum level of GHRL, and GH.
El-Magd MA, Saleh AA, Abdel-Hamid TM, Saleh RM, Afifi MA
General and comparative endocrinology237131-139(2016 Oct)

Obestatin directly controls chicken ovarian cell functions.
Sirotkin AV, M?sz?rosov? M, Harrath AH, Grossmann R
Journal of biological regulators and homeostatic agents30(3)665-674(2016 Jul-Sep)

Interrelationship between feeding level and the metabolic hormones leptin, ghrelin and obestatin in control of chicken egg laying and release of ovarian hormones.
Sirotkin AV, Grossmann R
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology1841-5(2015 Jun)

Correlation of ghrelin concentration and ghrelin, ghrelin-O-acetyltransferase (GOAT) and growth hormone secretagogue receptor 1a mRNAs expression in the proventriculus and brain of the growing chicken.
Kitazawa T, Hiraga T, Teraoka H, Yaosaka N, Kaiya H
Peptides63134-42(2015 Jan)

Gabaergic control of anxiety-like behavior, but not food intake, induced by ghrelin in the intermediate medial mesopallium of the neonatal chick.
Gast?n MS, Schi?th HB, De Barioglio SR, Salvatierra NA
Hormones and behavior6766-72(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This is the first study to demonstrate that single nucleotide polymorphisms in GHSR can increase appetite, growth traits, expression and level of GHRL, suggesting a hunger signal role for endogenous GHRL.
Title: Is really endogenous ghrelin a hunger signal in chickens? Association of GHSR SNPs with increase appetite, growth traits, expression and serum level of GHRL, and GH.

Obestatin can directly control avian ovarian cell proliferation, apoptosis and hormone release. The interrelationship between theca and granulosa cells can determine the characteristics of obestatin action on ovarian secretory activity.
Title: Obestatin directly controls chicken ovarian cell functions.

confirm the involvement of metabolic hormones leptin, ghrelin and obestatin in the control of chicken ovarian hormones output
Title: Interrelationship between feeding level and the metabolic hormones leptin, ghrelin and obestatin in control of chicken egg laying and release of ovarian hormones.

Expression levels of ghrelin, GOAT and GHS-R1a in the brain were independently regulated from that in the proventriculus in male chickens.
Title: Correlation of ghrelin concentration and ghrelin, ghrelin-O-acetyltransferase (GOAT) and growth hormone secretagogue receptor 1a mRNAs expression in the proventriculus and brain of the growing chicken.

results indicate that Ghr plays an important role in the response pattern to acute stressor, involving the possible participation of the GABAergic system.
Title: Gabaergic control of anxiety-like behavior, but not food intake, induced by ghrelin in the intermediate medial mesopallium of the neonatal chick.

The following GHRL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GHRL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa00043	NM_001001131.1 Latest version!	Gallus gallus ghrelin/obestatin prepropeptide (GHRL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OGa41395	XM_015293041.1 Latest version!	Gallus gallus ghrelin/obestatin prepropeptide (GHRL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GHRL ( NM_001001131.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001001131.1 Gallus gallus ghrelin/obestatin prepropeptide (GHRL), mRNA.

Price & Availability↑

CloneID	OGa00043
Clone ID Related Accession (Same CDS sequence)	NM_001001131.1
Accession Version	NM_001001131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-20
Organism	Gallus gallus(chicken)
Product	appetite-regulating hormone preproprotein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY299454.1. On Aug 30, 2004 this sequence version replaced XM_414449.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY299454.1, BX931092.2 [ECO:0000332] ##Evidence-Data-END##

ATGTTTCTCA GAGTTATTCT GCTAGGAATT CTCCTTCTCA GCATCCTTGG GACAGAAACT 
GCTCTGGCTG GCTCTAGTTT TTTAAGCCCC ACATATAAAA ACATACAGCA ACAAAAGGAT 
ACAAGAAAAC CAACAGCAAG ATTACATCGC CGAGGCACAG AAAGCTTTTG GGATACAGAT 
GAAACAGAGG GAGAAGATGA CAATAACAGT GTTGATATCA AGTTTAACGT TCCTTTTGAA 
ATTGGTGTCA AGATAACAGA AAGAGAGTAT CAAGAGTATG GACAAGCACT GGAGAAGATG 
CTACAGGACA TTCTTGCAGA GAATGCTGAA GAAACTCGGA CAAAAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001131.1
CDS	155..505
Translation	MFLRVILLGILLLSILGTETALAGSSFLSPTYKNIQQQKDTRKPTARLHRRGTESFWDTDETEGEDDNNSVDIKFNVPFEIGVKITEREYQEYGQALEKMLQDILAENAEETRTKS

Target ORF information:

RefSeq Version	NM_001001131.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ghrelin/obestatin prepropeptide (GHRL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001131.1

ORF Insert Sequence:

ATGTTTCTCA GAGTTATTCT GCTAGGAATT CTCCTTCTCA GCATCCTTGG GACAGAAACT 
GCTCTGGCTG GCTCTAGTTT TTTAAGCCCC ACATATAAAA ACATACAGCA ACAAAAGGAT 
ACAAGAAAAC CAACAGCAAG ATTACATCGC CGAGGCACAG AAAGCTTTTG GGATACAGAT 
GAAACAGAGG GAGAAGATGA CAATAACAGT GTTGATATCA AGTTTAACGT TCCTTTTGAA 
ATTGGTGTCA AGATAACAGA AAGAGAGTAT CAAGAGTATG GACAAGCACT GGAGAAGATG 
CTACAGGACA TTCTTGCAGA GAATGCTGAA GAAACTCGGA CAAAAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GHRL ( XM_015293041.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148527.1 Gallus gallus ghrelin/obestatin prepropeptide (GHRL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa41395
Clone ID Related Accession (Same CDS sequence)	XM_015293041.1
Accession Version	XM_015293041.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	appetite-regulating hormone isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTCA GAGTTATTCT GCTAGGAATT CTCCTTCTCA GCATCCTTGG GACAGAAACT 
GCTCTGGCTG GCTCTAGTTT TTTAAGCCCC ACATATAAAA ACATACAGCA ACAAAAGGAT 
ACAAGAAAAC CAACAGCAAG ATTACATCGC CGAGGCACAG AAAGCTTTTG GGATACAGAT 
GAAACAGAGG GAGAAGATGA CAATAACAGT GTTGATATCA AGTTTAACGT TCCTTTTGAA 
ATTGGTGTCA AGATAACAGA AAGAGAGTAT CAAGAGTATG GACAAGCACT GGAGAAGATG 
CTACAGGACA TTCTTGCAGA GAATGCTGAA GAAACTCAGA CAAAAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148527.1
CDS	1661..2011
Translation	MFLRVILLGILLLSILGTETALAGSSFLSPTYKNIQQQKDTRKPTARLHRRGTESFWDTDETEGEDDNNSVDIKFNVPFEIGVKITEREYQEYGQALEKMLQDILAENAEETQTKS

Target ORF information:

RefSeq Version	XM_015293041.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus ghrelin/obestatin prepropeptide (GHRL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293041.1

ORF Insert Sequence:

ATGTTTCTCA GAGTTATTCT GCTAGGAATT CTCCTTCTCA GCATCCTTGG GACAGAAACT 
GCTCTGGCTG GCTCTAGTTT TTTAAGCCCC ACATATAAAA ACATACAGCA ACAAAAGGAT 
ACAAGAAAAC CAACAGCAAG ATTACATCGC CGAGGCACAG AAAGCTTTTG GGATACAGAT 
GAAACAGAGG GAGAAGATGA CAATAACAGT GTTGATATCA AGTTTAACGT TCCTTTTGAA 
ATTGGTGTCA AGATAACAGA AAGAGAGTAT CAAGAGTATG GACAAGCACT GGAGAAGATG 
CTACAGGACA TTCTTGCAGA GAATGCTGAA GAAACTCAGA CAAAAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Is really endogenous ghrelin a hunger signal in chickens? Association of GHSR SNPs with increase appetite, growth traits, expression and serum level of GHRL, and GH.
General and comparative endocrinology237131-139(2016 Oct)
El-Magd MA,Saleh AA,Abdel-Hamid TM,Saleh RM,Afifi MA

Obestatin directly controls chicken ovarian cell functions.
Journal of biological regulators and homeostatic agents30(3)665-674(2016 Jul-Sep)
Sirotkin AV,M?sz?rosov? M,Harrath AH,Grossmann R

Interrelationship between feeding level and the metabolic hormones leptin, ghrelin and obestatin in control of chicken egg laying and release of ovarian hormones.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology1841-5(2015 Jun)
Sirotkin AV,Grossmann R

Correlation of ghrelin concentration and ghrelin, ghrelin-O-acetyltransferase (GOAT) and growth hormone secretagogue receptor 1a mRNAs expression in the proventriculus and brain of the growing chicken.
Peptides63134-42(2015 Jan)
Kitazawa T,Hiraga T,Teraoka H,Yaosaka N,Kaiya H

Gabaergic control of anxiety-like behavior, but not food intake, induced by ghrelin in the intermediate medial mesopallium of the neonatal chick.
Hormones and behavior6766-72(2015 Jan)
Gast?n MS,Schi?th HB,De Barioglio SR,Salvatierra NA