H257_02472 cDNA ORF clone, Aphanomyces astaci

Gene
Clones
gRNAs

Summary

Gene Symbol	H257_02472
Entrez Gene ID	20804468
Full Name	valine-tRNA ligase
Gene Type	protein-coding
Organism	Aphanomyces astaci

mRNA and Protein(s)

mRNA	Protein	Name
XM_009826136.1	XP_009824438.1	valine-tRNA ligase

Pathways from BioSystems
Gene Ontology

The following H257_02472 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H257_02472 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAp98879	XM_009826136.1 Latest version!	Aphanomyces astaci valine-tRNA ligase partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H257_02472 ( XM_009826136.1 ) cDNA ORF clone, Aphanomyces astaci -> XP_009824438.1 Aphanomyces astaci valine-tRNA ligase partial mRNA.

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CloneID	OAp98879
Clone ID Related Accession (Same CDS sequence)	XM_009826136.1
Accession Version	XM_009826136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-20
Organism	Aphanomyces astaci
Product	valine-tRNA ligase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_009798267).

ATGTTGATGC GAGCATGGCA AGCGTCCAAG GGCCGTCCGT TCGTGCGGGT GTCCCGAAGT 
TTGACTTCCC TGCCCGACCA CATCAAGCAG CGGGTGCATG TTGATCCCAT GTCCTCCACG 
TACGATCCAC TTGCTGTCGA GGACGGATGG CAGGCGTTTT GGGAAAGCAA ACTCGTCGAC 
GCCAAGAAAG CCAACCCCGA CGCCCCGCGC TTCACGATGC TTCTGCCACC GCCCAACATC 
ACCGGCGCTC TGCATATAGG CCATGCGCTG ACCGTGACGA TCCAAGATGC GCTAGCTCGG 
TATCATCGTA TGTGTGGTCA TGAAGTGCTG TGGATCCCAG GCCTGGACCA CGCCGGCATC 
GCCACGCAAA GCGTCGTGGA GAAGCAGCTG TGGAAGGAAC ACAAGCTCAC GCGACACGAT 
GTCGGCCGCA GTGCATTTCT AGACCACGTG CATGGTTGGA ACGCTACCTA CGGTAGTCGC 
ATTCTGAACC AATTGGAGCG ACTGGGCGCT CTCTTGAACA AGGACCACAC GTTTTTCACG 
CTCGACGACG CACGGTCGAA GGCCGTGGTG GATGCATTCT GCCGCCTGCA TGCAAAAGGG 
CTGGTGTATC GGCATCGGCG TATGGTCAAC TGGTGCCCGA CACTGCAAAC GGCCATCTCG 
GACATTGAAG TCGACGCCGT GCCACTGACC AAACGCACAA TGCTGCCGCT TCCTGGCCAC 
GCCACGCCAG TGGAATTTGG TGTCATGCAT CGGTTCAAGT ACAAAGTGGA TCGATCCGAC 
TCGGTCGTTG CTGACACTGA GTTTGTCCAC GTCGACACGA CGCGACCGGA AACAATTCTG 
GGGGACGTGG CCCTCGCCAT CCACCCAGAT GACGCGCGGT ACTTGCATTT GCACGGCCAG 
CATGTCGTCC ACCCGTTCAC AAACGAGCGG CTGCCCATCG TTCTGGACGC GCAATTGGTC 
GATCCAACCC TCGGCACTGG CGTCGTCAAG CTCACACCGG CGCACGACGC CAACGACTGG 
GCGTGTGCGC AGCGTCACAA CCTCCCCCAC GTCGTCGTCA TGGACAAACT AGCCAAGATG 
GTCACGCCCA ACGTTCCCGC CTTCCACGGC CTGGATCGGT TTGACGCTCG CGCCGCGATG 
GTCGCCAAAC TGCAAGAGCT CGATCTGTAC GTGGAAAAGC TGGACCATCC GACGTCCGTG 
TCCATCTGTT CGCGGTCAGG GAACGTCGTC GAGCCATATT TAATGCCCCA GTGGTTTGTC 
CAGTGCGACG GCATGGCCGC GGCGTCCGCA CAGGCCGTTC GAAGCAATGC CTTGGCGTTA 
GAACCAGCGA CGCACCACCA CACGTGGTTC CACTACTTGG ACAATATCCA GGACTGGTGT 
GTGTCTCGGC AGTTGTGGTG GGGCCACCGC ATCCCCGCCT ACCGCGTGCA TTTGGCTGGC 
CACCAAGCCT CTGACGACAA TGACGATAAC TTGGAGAACG AGCGGTGGGT CGTAGCTCGC 
AACGAAGTCG AAGCCCGGCA GTTGGCCTTG GAAACGTACG GCGACCATCC GCTGCTGACG 
CTGGAACAAG ACCAAGACGT GCTGGACACG TGGTTCAGCT CGGCGTTGCT GCCGCTGTCG 
GCGCTGGAAT GGCCGACCGA TGACACTGTT CCTGAAACTA TCCAACACGT GTACCCGTTG 
AGCGTCATGG AGACTGGAGC CGACATCTTG TTCTTCTGGG TCGCGCGCAT GAGTATGCTG 
TGTACGGCGT TGAGTGGACG CCAGCCGTTT GATAAAGTAT GGTTGCATCC CATGGTGCGT 
GACAAAAGCG GTCGAAAGAT GTCGAAGAGT CTCGGCAATG TCATCGATCC ACTGCACGTG 
ATTGCCGGCA TCGACCTCCC AACCCTCGTC CAAGGCCTCC ACCAAGGCAA CCTCGACCCG 
AAGGAGCTCG TGCGTGCGGA AAAGGACTTG AAGCGCGAGT TTCCGTCTGG GATTCCCAGC 
TGCGGCACGG ACGCGTTGCG GCTCACGCTC TCCACGTACT TGGCGCAAGG ACGCCAAATC 
AACATGGATT TGCAGCGGGT GGTGGCATCG CGGCACTTTT GCAATAAAAT GTGGAACGCA 
TTTCGGTATG CGTTGCCGCT GCTGCCAGCT CCTCATCACT CTGGGCGGCA TGGGGGGGGG 
TCTACGTTGG AGGACCTGCG TCCGCACATG GGGCTTGCCG AGCGCTGGAT TCTCAGTCGC 
ATGGCGGACG CCGTGAGTAA ATCGCACGAG GGATTTGAGT CGTTTCGACT GGCACAAAGC 
GCCGGCGCTG CCCAGCGCTT CTTCTTGCAA GACCTGTGCG ACGTGTACAT TGAATTCTCC 
AAGCCCGTCT TGTACACTGC CAAGAACAAC CATGATGACG GTGGTGGTGG GGATGGTGAT 
GATTTGGTGG TGGCGGCAGC GGATAGAGGC GACGCGGCCC GTCGACAGGA AAGCGCGCAA 
GCCACTCTGC GACAGTGTTT GGACACGTCC GTGCGGCTCT TGCATCCGTT CATGCCGTTC 
GTGACGGAAG AGCTGTGGCA ACGTTTGACG TGGTCCGCCG ATGGTACGAC TACTGGCGGC 
GACTCGCTGA CTCTGGCGGC GTTCCCGTGC AAGCAAGACG TGGCCACATG GCGGGATGTA 
GATGCTGAAG AAAGCATGCA GGCGGTGTTG GACGTCATGC ACGCCATGCG ATCCCTCAAG 
CACACTCGAA AGACGCTTGT GCCAGACCAT GACGACAGCA TGGACACTGG ACACTTGACC 
GTGCACTGTA CCGACCCGAC GCTCACGGCC CTCCTCGAAC GCAACATGGC GGACATTCGC 
ACGCAATGCC GAAGTGGCGT CCACTTGGTG TCGGCGGCGC CGTCGGACGG CACGTACCTG 
TCTCAGTCGA TTTCGACCTC TTGTCAAGTG TGGATGCCAC TGCGCGCGAC GGCCAACACG 
TCGGAACGCC TGGACGCAGA GCTCAGTCGA CTCATGAAGC GGTTGGCCAA GGCGGAAAAG 
ACCAAGGCGA GTCTTGATGC AAAGGTAGCC GACCCACTCT ACACGACCCG CGTGCCCGTC 
GAAATCCAAG CGCAGGACGC CGAGCGCATC CAAGATGCCG CCGTGGAGAT TGCGACCTTG 
ACCGACAGCA CGACCACGTT ATGCACGTTG AAAGTGCAAT TAACCACCAA ATCCACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009824438.1
CDS	1..3180
Translation	MLMRAWQASKGRPFVRVSRSLTSLPDHIKQRVHVDPMSSTYDPLAVEDGWQAFWESKLVDAKKANPDAPRFTMLLPPPNITGALHIGHALTVTIQDALARYHRMCGHEVLWIPGLDHAGIATQSVVEKQLWKEHKLTRHDVGRSAFLDHVHGWNATYGSRILNQLERLGALLNKDHTFFTLDDARSKAVVDAFCRLHAKGLVYRHRRMVNWCPTLQTAISDIEVDAVPLTKRTMLPLPGHATPVEFGVMHRFKYKVDRSDSVVADTEFVHVDTTRPETILGDVALAIHPDDARYLHLHGQHVVHPFTNERLPIVLDAQLVDPTLGTGVVKLTPAHDANDWACAQRHNLPHVVVMDKLAKMVTPNVPAFHGLDRFDARAAMVAKLQELDLYVEKLDHPTSVSICSRSGNVVEPYLMPQWFVQCDGMAAASAQAVRSNALALEPATHHHTWFHYLDNIQDWCVSRQLWWGHRIPAYRVHLAGHQASDDNDDNLENERWVVARNEVEARQLALETYGDHPLLTLEQDQDVLDTWFSSALLPLSALEWPTDDTVPETIQHVYPLSVMETGADILFFWVARMSMLCTALSGRQPFDKVWLHPMVRDKSGRKMSKSLGNVIDPLHVIAGIDLPTLVQGLHQGNLDPKELVRAEKDLKREFPSGIPSCGTDALRLTLSTYLAQGRQINMDLQRVVASRHFCNKMWNAFRYALPLLPAPHHSGRHGGGSTLEDLRPHMGLAERWILSRMADAVSKSHEGFESFRLAQSAGAAQRFFLQDLCDVYIEFSKPVLYTAKNNHDDGGGGDGDDLVVAAADRGDAARRQESAQATLRQCLDTSVRLLHPFMPFVTEELWQRLTWSADGTTTGGDSLTLAAFPCKQDVATWRDVDAEESMQAVLDVMHAMRSLKHTRKTLVPDHDDSMDTGHLTVHCTDPTLTALLERNMADIRTQCRSGVHLVSAAPSDGTYLSQSISTSCQVWMPLRATANTSERLDAELSRLMKRLAKAEKTKASLDAKVADPLYTTRVPVEIQAQDAERIQDAAVEIATLTDSTTTLCTLKVQLTTKST

Target ORF information:

RefSeq Version	XM_009826136.1
Organism	Aphanomyces astaci
Definition	Aphanomyces astaci valine-tRNA ligase partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009826136.1

ORF Insert Sequence:

ATGTTGATGC GAGCATGGCA AGCGTCCAAG GGCCGTCCGT TCGTGCGGGT GTCCCGAAGT 
TTGACTTCCC TGCCCGACCA CATCAAGCAG CGGGTGCATG TTGATCCCAT GTCCTCCACG 
TACGATCCAC TTGCTGTCGA GGACGGATGG CAGGCGTTTT GGGAAAGCAA ACTCGTCGAC 
GCCAAGAAAG CCAACCCCGA CGCCCCGCGC TTCACGATGC TTCTGCCACC GCCCAACATC 
ACCGGCGCTC TGCATATAGG CCATGCGCTG ACCGTGACGA TCCAAGATGC GCTAGCTCGG 
TATCATCGTA TGTGTGGTCA TGAAGTGCTG TGGATCCCAG GCCTGGACCA CGCCGGCATC 
GCCACGCAAA GCGTCGTGGA GAAGCAGCTG TGGAAGGAAC ACAAGCTCAC GCGACACGAT 
GTCGGCCGCA GTGCATTTCT AGACCACGTG CATGGTTGGA ACGCTACCTA CGGTAGTCGC 
ATTCTGAACC AATTGGAGCG ACTGGGCGCT CTCTTGAACA AGGACCACAC GTTTTTCACG 
CTCGACGACG CACGGTCGAA GGCCGTGGTG GATGCATTCT GCCGCCTGCA TGCAAAAGGG 
CTGGTGTATC GGCATCGGCG TATGGTCAAC TGGTGCCCGA CACTGCAAAC GGCCATCTCG 
GACATTGAAG TCGACGCCGT GCCACTGACC AAACGCACAA TGCTGCCGCT TCCTGGCCAC 
GCCACGCCAG TGGAATTTGG TGTCATGCAT CGGTTCAAGT ACAAAGTGGA TCGATCCGAC 
TCGGTCGTTG CTGACACTGA GTTTGTCCAC GTCGACACGA CGCGACCGGA AACAATTCTG 
GGGGACGTGG CCCTCGCCAT CCACCCAGAT GACGCGCGGT ACTTGCATTT GCACGGCCAG 
CATGTCGTCC ACCCGTTCAC AAACGAGCGG CTGCCCATCG TTCTGGACGC GCAATTGGTC 
GATCCAACCC TCGGCACTGG CGTCGTCAAG CTCACACCGG CGCACGACGC CAACGACTGG 
GCGTGTGCGC AGCGTCACAA CCTCCCCCAC GTCGTCGTCA TGGACAAACT AGCCAAGATG 
GTCACGCCCA ACGTTCCCGC CTTCCACGGC CTGGATCGGT TTGACGCTCG CGCCGCGATG 
GTCGCCAAAC TGCAAGAGCT CGATCTGTAC GTGGAAAAGC TGGACCATCC GACGTCCGTG 
TCCATCTGTT CGCGGTCAGG GAACGTCGTC GAGCCATATT TAATGCCCCA GTGGTTTGTC 
CAGTGCGACG GCATGGCCGC GGCGTCCGCA CAGGCCGTTC GAAGCAATGC CTTGGCGTTA 
GAACCAGCGA CGCACCACCA CACGTGGTTC CACTACTTGG ACAATATCCA GGACTGGTGT 
GTGTCTCGGC AGTTGTGGTG GGGCCACCGC ATCCCCGCCT ACCGCGTGCA TTTGGCTGGC 
CACCAAGCCT CTGACGACAA TGACGATAAC TTGGAGAACG AGCGGTGGGT CGTAGCTCGC 
AACGAAGTCG AAGCCCGGCA GTTGGCCTTG GAAACGTACG GCGACCATCC GCTGCTGACG 
CTGGAACAAG ACCAAGACGT GCTGGACACG TGGTTCAGCT CGGCGTTGCT GCCGCTGTCG 
GCGCTGGAAT GGCCGACCGA TGACACTGTT CCTGAAACTA TCCAACACGT GTACCCGTTG 
AGCGTCATGG AGACTGGAGC CGACATCTTG TTCTTCTGGG TCGCGCGCAT GAGTATGCTG 
TGTACGGCGT TGAGTGGACG CCAGCCGTTT GATAAAGTAT GGTTGCATCC CATGGTGCGT 
GACAAAAGCG GTCGAAAGAT GTCGAAGAGT CTCGGCAATG TCATCGATCC ACTGCACGTG 
ATTGCCGGCA TCGACCTCCC AACCCTCGTC CAAGGCCTCC ACCAAGGCAA CCTCGACCCG 
AAGGAGCTCG TGCGTGCGGA AAAGGACTTG AAGCGCGAGT TTCCGTCTGG GATTCCCAGC 
TGCGGCACGG ACGCGTTGCG GCTCACGCTC TCCACGTACT TGGCGCAAGG ACGCCAAATC 
AACATGGATT TGCAGCGGGT GGTGGCATCG CGGCACTTTT GCAATAAAAT GTGGAACGCA 
TTTCGGTATG CGTTGCCGCT GCTGCCAGCT CCTCATCACT CTGGGCGGCA TGGGGGGGGG 
TCTACGTTGG AGGACCTGCG TCCGCACATG GGGCTTGCCG AGCGCTGGAT TCTCAGTCGC 
ATGGCGGACG CCGTGAGTAA ATCGCACGAG GGATTTGAGT CGTTTCGACT GGCACAAAGC 
GCCGGCGCTG CCCAGCGCTT CTTCTTGCAA GACCTGTGCG ACGTGTACAT TGAATTCTCC 
AAGCCCGTCT TGTACACTGC CAAGAACAAC CATGATGACG GTGGTGGTGG GGATGGTGAT 
GATTTGGTGG TGGCGGCAGC GGATAGAGGC GACGCGGCCC GTCGACAGGA AAGCGCGCAA 
GCCACTCTGC GACAGTGTTT GGACACGTCC GTGCGGCTCT TGCATCCGTT CATGCCGTTC 
GTGACGGAAG AGCTGTGGCA ACGTTTGACG TGGTCCGCCG ATGGTACGAC TACTGGCGGC 
GACTCGCTGA CTCTGGCGGC GTTCCCGTGC AAGCAAGACG TGGCCACATG GCGGGATGTA 
GATGCTGAAG AAAGCATGCA GGCGGTGTTG GACGTCATGC ACGCCATGCG ATCCCTCAAG 
CACACTCGAA AGACGCTTGT GCCAGACCAT GACGACAGCA TGGACACTGG ACACTTGACC 
GTGCACTGTA CCGACCCGAC GCTCACGGCC CTCCTCGAAC GCAACATGGC GGACATTCGC 
ACGCAATGCC GAAGTGGCGT CCACTTGGTG TCGGCGGCGC CGTCGGACGG CACGTACCTG 
TCTCAGTCGA TTTCGACCTC TTGTCAAGTG TGGATGCCAC TGCGCGCGAC GGCCAACACG 
TCGGAACGCC TGGACGCAGA GCTCAGTCGA CTCATGAAGC GGTTGGCCAA GGCGGAAAAG 
ACCAAGGCGA GTCTTGATGC AAAGGTAGCC GACCCACTCT ACACGACCCG CGTGCCCGTC 
GAAATCCAAG CGCAGGACGC CGAGCGCATC CAAGATGCCG CCGTGGAGAT TGCGACCTTG 
ACCGACAGCA CGACCACGTT ATGCACGTTG AAAGTGCAAT TAACCACCAA ATCCACGTAG