Hsc70-4 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsc70-4
Entrez Gene ID	409418
Full Name	heat shock protein cognate 4
Synonyms	GB14852
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001160050.1	NP_001153522.1	heat shock protein cognate 4

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
Elsik CG, Worley KC, Bennett AK, Beye M, Camara F, Childers CP, de Graaf DC, Debyser G, Deng J, Devreese B, Elhaik E, Evans JD, Foster LJ, Graur D, Guigo R, , Hoff KJ, Holder ME, Hudson ME, Hunt GJ, Jiang H, Joshi V, Khetani RS, Kosarev P, Kovar CL, Ma J, Maleszka R, Moritz RF, Munoz-Torres MC, Murphy TD, Muzny DM, Newsham IF, Reese JT, Robertson HM, Robinson GE, Rueppell O, Solovyev V, Stanke M, Stolle E, Tsuruda JM, Vaerenbergh MV, Waterhouse RM, Weaver DB, Whitfield CW, Wu Y, Zdobnov EM, Zhang L, Zhu D, Gibbs RA,
BMC genomics1586(2014 Jan)

Age and natural metabolically-intensive behavior affect oxidative stress and antioxidant mechanisms.
Williams JB, Roberts SP, Elekonich MM
Experimental gerontology43(6)538-49(2008 Jun)

Insights into social insects from the genome of the honeybee Apis mellifera.

Nature443(7114)931-49(2006 Oct)

Annotated expressed sequence tags and cDNA microarrays for studies of brain and behavior in the honey bee.
Whitfield CW, Band MR, Bonaldo MF, Kumar CG, Liu L, Pardinas JR, Robertson HM, Soares MB, Robinson GE
Genome research12(4)555-66(2002 Apr)

Evaluation of differential gene expression during behavioral development in the honeybee using microarrays and northern blots.
Kucharski R, Maleszka R
Genome biology3(2)RESEARCH0007(2002)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hsc70-4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsc70-4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHd00317	NM_001160050.1 Latest version!	Apis mellifera heat shock protein cognate 4 (Hsc70-4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsc70-4 ( NM_001160050.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> NP_001153522.1 Apis mellifera heat shock protein cognate 4 (Hsc70-4), mRNA.

Price & Availability↑

CloneID	OHd00317
Clone ID Related Accession (Same CDS sequence)	NM_001160050.1
Accession Version	NM_001160050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-18
Organism	Apis mellifera(honey bee)
Product	heat shock protein cognate 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB728522.1, BI510653.1, DB737689.1, BI946540.1, BI504420.1, QIUM02000001.1, DB764715.1 and DB761554.1. On May 10, 2009 this sequence version replaced XM_392933.3. ##Evidence-Data-START## Transcript exon combination :: GAZV01015615.1, HP566771.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00120364, SAMN00996631 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTAAAG CACCTGCAGT TGGTATTGAT CTTGGCACTA CTTATTCCTG TGTTGGTGTA 
TTCCAGCATG GAAAAGTAGA AATCATCGCT AATGATCAAG GAAATCGTAC AACACCTAGC 
TATGTTGCAT TTACGGAAAC TGAACGATTA ATCGGCGATG CTGCCAAGAA CCAAGTTGCC 
ATGAACCCCA ACAACACCAT TTTCGATGCC AAACGTCTTA TTGGTCGTCG TTTTGAAGAT 
CCCACTGTTC AGGCTGATAT GAAGCATTGG CCCTTTACTG TTGTAAATGA TGGTGGCAAG 
CCTAAAATTC AAGTATATTA CAAAGGAGAG GCGAAGACTT TCTTCCCCGA AGAAGTGAGT 
TCAATGGTCC TGGTAAAGAT GAAGGAAACA GCTGAAGCAT ATCTGGGAAA AACTGTCAGT 
AATGCCGTGA TCACTGTACC TGCTTACTTC AACGACTCGC AGCGTCAGGC AACTAAGGAC 
GCTGGTACCA TCTCGGGCTT AAATGTTTTA CGTATTATTA ATGAGCCCAC CGCTGCTGCC 
ATTGCTTATG GCTTGGATAA AAAAACTACC AGCGAACGCA ACGTCCTTAT CTTTGATCTT 
GGTGGTGGCA CCTTTGATGT CTCAATTCTG ACCATTGAAG ATGGCATTTT CGAGGTCAAG 
TCAACAGCAG GAGATACCCA TCTTGGCGGC GAAGATTTTG ATAACCGTAT GGTTAATCAC 
TTTGTACAAG AATTCAAACG GAAATATAAA AAAGATTTAA CTGCAAATAA ACGCGCTCTT 
CGTCGTTTGA GAACAGCTTG TGAGCGTGCG AAACGCACTC TGTCTTCGTC TACTCAAGCT 
AGCATTGAAA TTGATTCGCT CTATGAGGGA ATCGATTTTT ATACATCGAT CACCAGAGCT 
CGATTTGAAG AACTCTGTGC TGATCTCTTC AGAGGAACAC TCGAGCCAGT AGAAAAATCA 
TTACGCGATG CTAAAATGGA TAAAGCTCAG ATTCACGATA TCGTTCTGGT TGGTGGATCC 
ACGCGAATTC CTAAGATTCA AAAACTTCTG CAAGACTTCT TCAATGGAAA GGAATTGAAT 
AAATCTATCA ATCCTGATGA GGCTGTGGCA TATGGAGCTG CTGTGCAAGC TGCCATTCTT 
CATGGTGATA AATCTGAAGA AGTACAAGAT CTATTACTTC TTGATGTAAC ACCTCTATCT 
CTTGGTATTG AAACTGCTGG TGGTGTTATG ACTGCTCTTA TTAAGAGAAA TACTACCATT 
CCAACAAAGC AAACTCAAAC CTTCACTACA TATGCTGATA ATCAACCAGG TGTATTGATT 
CAAGTCTATG AAGGAGAACG TGCTATGACG AAAGATAACA ATCTGTTAGG TAAATTTGAG 
CTCAGCGGCA TTCCTCCAGC TCCTCGAGGA GTTCCTCAAA TTGAAGTCAC CTTTGATATC 
GATGCCAATG GTATTCTCAA TGTCTCTGCA GTTGATAAAT CTACTGGTAA AGAGAACAAA 
ATCACTATTA CCAACGATAA AGGTCGTCTC AGCAAAGAAG ATATCGAAAG GATGGTCAAC 
GAAGCTGAGA AATATCGTAG CGAAGACGAA AAACAAAAAG AAACTATCGC TGCTAAGAAT 
GGTCTTGAGT CTTACTGCTT CAACATGAAG AGTACAGTGG AAGACGAGAA GTTGAAGGAT 
AAAATCAGCG CCAGTGACAA ACAAGTTGTA CTGGATAAAT GCAACGATAT CATTAAATGG 
CTCGATGCTA ACCAATTAGC TGATAAAGAA GAATACGAAC ATAAACAGAA AGAATTAGAA 
GCTATATGCA ATCCTATTGT CACAAAATTG TATCAGGGCA CTGGTGGGAT GCCTGGTGGA 
ATGCCTGGTG GAATGCCTGG TGGCTTCCCA GGAGCTGGTG GCGGTGCTCC TGGTGGTGGT 
GCATCTGGAC CTACCATCGA AGAAGTCGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153522.1
CDS	104..2056
Translation	MAKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTETERLIGDAAKNQVAMNPNNTIFDAKRLIGRRFEDPTVQADMKHWPFTVVNDGGKPKIQVYYKGEAKTFFPEEVSSMVLVKMKETAEAYLGKTVSNAVITVPAYFNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKTTSERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFVQEFKRKYKKDLTANKRALRRLRTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELCADLFRGTLEPVEKSLRDAKMDKAQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILHGDKSEEVQDLLLLDVTPLSLGIETAGGVMTALIKRNTTIPTKQTQTFTTYADNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVNEAEKYRSEDEKQKETIAAKNGLESYCFNMKSTVEDEKLKDKISASDKQVVLDKCNDIIKWLDANQLADKEEYEHKQKELEAICNPIVTKLYQGTGGMPGGMPGGMPGGFPGAGGGAPGGGASGPTIEEVD

Target ORF information:

RefSeq Version	NM_001160050.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera heat shock protein cognate 4 (Hsc70-4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160050.1

ORF Insert Sequence:

ATGGCTAAAG CACCTGCAGT TGGTATTGAT CTTGGCACTA CTTATTCCTG TGTTGGTGTA 
TTCCAGCATG GAAAAGTAGA AATCATCGCT AATGATCAAG GAAATCGTAC AACACCTAGC 
TATGTTGCAT TTACGGAAAC TGAACGATTA ATCGGCGATG CTGCCAAGAA CCAAGTTGCC 
ATGAACCCCA ACAACACCAT TTTCGATGCC AAACGTCTTA TTGGTCGTCG TTTTGAAGAT 
CCCACTGTTC AGGCTGATAT GAAGCATTGG CCCTTTACTG TTGTAAATGA TGGTGGCAAG 
CCTAAAATTC AAGTATATTA CAAAGGAGAG GCGAAGACTT TCTTCCCCGA AGAAGTGAGT 
TCAATGGTCC TGGTAAAGAT GAAGGAAACA GCTGAAGCAT ATCTGGGAAA AACTGTCAGT 
AATGCCGTGA TCACTGTACC TGCTTACTTC AACGACTCGC AGCGTCAGGC AACTAAGGAC 
GCTGGTACCA TCTCGGGCTT AAATGTTTTA CGTATTATTA ATGAGCCCAC CGCTGCTGCC 
ATTGCTTATG GCTTGGATAA AAAAACTACC AGCGAACGCA ACGTCCTTAT CTTTGATCTT 
GGTGGTGGCA CCTTTGATGT CTCAATTCTG ACCATTGAAG ATGGCATTTT CGAGGTCAAG 
TCAACAGCAG GAGATACCCA TCTTGGCGGC GAAGATTTTG ATAACCGTAT GGTTAATCAC 
TTTGTACAAG AATTCAAACG GAAATATAAA AAAGATTTAA CTGCAAATAA ACGCGCTCTT 
CGTCGTTTGA GAACAGCTTG TGAGCGTGCG AAACGCACTC TGTCTTCGTC TACTCAAGCT 
AGCATTGAAA TTGATTCGCT CTATGAGGGA ATCGATTTTT ATACATCGAT CACCAGAGCT 
CGATTTGAAG AACTCTGTGC TGATCTCTTC AGAGGAACAC TCGAGCCAGT AGAAAAATCA 
TTACGCGATG CTAAAATGGA TAAAGCTCAG ATTCACGATA TCGTTCTGGT TGGTGGATCC 
ACGCGAATTC CTAAGATTCA AAAACTTCTG CAAGACTTCT TCAATGGAAA GGAATTGAAT 
AAATCTATCA ATCCTGATGA GGCTGTGGCA TATGGAGCTG CTGTGCAAGC TGCCATTCTT 
CATGGTGATA AATCTGAAGA AGTACAAGAT CTATTACTTC TTGATGTAAC ACCTCTATCT 
CTTGGTATTG AAACTGCTGG TGGTGTTATG ACTGCTCTTA TTAAGAGAAA TACTACCATT 
CCAACAAAGC AAACTCAAAC CTTCACTACA TATGCTGATA ATCAACCAGG TGTATTGATT 
CAAGTCTATG AAGGAGAACG TGCTATGACG AAAGATAACA ATCTGTTAGG TAAATTTGAG 
CTCAGCGGCA TTCCTCCAGC TCCTCGAGGA GTTCCTCAAA TTGAAGTCAC CTTTGATATC 
GATGCCAATG GTATTCTCAA TGTCTCTGCA GTTGATAAAT CTACTGGTAA AGAGAACAAA 
ATCACTATTA CCAACGATAA AGGTCGTCTC AGCAAAGAAG ATATCGAAAG GATGGTCAAC 
GAAGCTGAGA AATATCGTAG CGAAGACGAA AAACAAAAAG AAACTATCGC TGCTAAGAAT 
GGTCTTGAGT CTTACTGCTT CAACATGAAG AGTACAGTGG AAGACGAGAA GTTGAAGGAT 
AAAATCAGCG CCAGTGACAA ACAAGTTGTA CTGGATAAAT GCAACGATAT CATTAAATGG 
CTCGATGCTA ACCAATTAGC TGATAAAGAA GAATACGAAC ATAAACAGAA AGAATTAGAA 
GCTATATGCA ATCCTATTGT CACAAAATTG TATCAGGGCA CTGGTGGGAT GCCTGGTGGA 
ATGCCTGGTG GAATGCCTGG TGGCTTCCCA GGAGCTGGTG GCGGTGCTCC TGGTGGTGGT 
GCATCTGGAC CTACCATCGA AGAAGTCGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Finding the missing honey bee genes: lessons learned from a genome upgrade.
BMC genomics1586(2014 Jan)
Elsik CG,Worley KC,Bennett AK,Beye M,Camara F,Childers CP,de Graaf DC,Debyser G,Deng J,Devreese B,Elhaik E,Evans JD,Foster LJ,Graur D,Guigo R,,Hoff KJ,Holder ME,Hudson ME,Hunt GJ,Jiang H,Joshi V,Khetani RS,Kosarev P,Kovar CL,Ma J,Maleszka R,Moritz RF,Munoz-Torres MC,Murphy TD,Muzny DM,Newsham IF,Reese JT,Robertson HM,Robinson GE,Rueppell O,Solovyev V,Stanke M,Stolle E,Tsuruda JM,Vaerenbergh MV,Waterhouse RM,Weaver DB,Whitfield CW,Wu Y,Zdobnov EM,Zhang L,Zhu D,Gibbs RA,

Age and natural metabolically-intensive behavior affect oxidative stress and antioxidant mechanisms.
Experimental gerontology43(6)538-49(2008 Jun)
Williams JB,Roberts SP,Elekonich MM

Insights into social insects from the genome of the honeybee Apis mellifera.
Nature443(7114)931-49(2006 Oct)

Annotated expressed sequence tags and cDNA microarrays for studies of brain and behavior in the honey bee.
Genome research12(4)555-66(2002 Apr)
Whitfield CW,Band MR,Bonaldo MF,Kumar CG,Liu L,Pardinas JR,Robertson HM,Soares MB,Robinson GE

Evaluation of differential gene expression during behavioral development in the honeybee using microarrays and northern blots.
Genome biology3(2)RESEARCH0007(2002)
Kucharski R,Maleszka R