LOC725665 cDNA ORF clone, Apis mellifera(honey bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC725665
Entrez Gene ID	725665
Full Name	5-oxoprolinase
Gene Type	protein-coding
Organism	Apis mellifera(honey bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016911641.2	XP_016767130.1	5-oxoprolinase isoform X2
XM_006567267.2	XP_006567330.1	5-oxoprolinase isoform X1
XM_016911641.1	XP_016767130.1	5-oxoprolinase isoform X2
XM_006567267.3	XP_006567330.1	5-oxoprolinase isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC725665 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC725665 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHd21416	XM_016911641.1 Latest version!	Apis mellifera 5-oxoprolinase (LOC725665), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OHd30405	XM_006567267.3 Latest version!	Apis mellifera 5-oxoprolinase (LOC725665), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHd30406	XM_016911641.2 Latest version!	Apis mellifera 5-oxoprolinase (LOC725665), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHd05681	XM_006567267.2 Latest version!	Apis mellifera 5-oxoprolinase (LOC725665), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC725665 ( XM_016911641.1 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_016767130.1 Apis mellifera 5-oxoprolinase (LOC725665), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHd21416
Clone ID Related Accession (Same CDS sequence)	XM_016911641.1
Accession Version	XM_016911641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-23
Organism	Apis mellifera(honey bee)
Product	5-oxoprolinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007073.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis mellifera Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAACGA CAGTCGCCAC GAACGCTCTT TTGGAAAGAA AAGGCGCGAA AATGGCGCTG 
TTGATCAACG AAGGATTCAA AGATCTTCTT TTCATCGGGA ATCAAGCACG CCCCGATATA 
TTCGACTTGC AAGTAGTCAC TCCGGAAGTT TTATACGAAA AGGTAATCGA AGTTAGGTGC 
AGAGTGATAC CCGCATCGGT TGGAAAGTGC CAGTTGGATA ACAAGAAATG GCGCAGAGTG 
AAGGGATGTA CGGGGGAGGA TCTGTTCATC ACTCGAGAAC TGGACGAGGA AAAGTTGAGA 
TTAGATCTGG AGGAATTGAA AAGATCGGGA ATCGAGAGTT TAGCCGTTGT CCTCGCGCAC 
AGTTACACAT ACGGCGAGCA CGAAATACGA GTAGGGGAAT TGGCGAAACT CGTTGGCTTC 
ACCCAAGTTT CGCTTTCGCA CGAAATCATG CCTATGGCGA GAATAGTTCC TAGAGGATTC 
ACAGCCTGCG CCGATGCATA CTTGACCCCG CACATTAAAC AATACTTACA GGGATTCTCT 
TCTGGTTTCA AAGATAGATT GAAAGATGTG AACGTGTTGT TCATGCAAAG CGACGGTGGA 
TTGACCCCGA TGAATTCATT CAATGGGTCC CGGGCCATTC TTTCCGGGCC AGCGGGCGGA 
GTCGTCGGTT ATGCGATCAC CACTTACGGC AAAGAGACCG ATTTGCCCGT GATTGGATTC 
GATATGGGCG GCACTTCTAC CGACGTCAGT CGTTTCGGTG GCAGCTACGA GCATGTGTAC 
GAGAGCACAA CCGCAGGCGT CACGATCCAA GCTGCGCAGT TGGACGTGAA CACAGTTGCG 
GCAGGGGGTG GATCGATGCT TTTCTTCAGG TCGGGTCTGT TCGAGGTCGG GCCCGAGAGC 
GCAGGGGCGC ATCCTGGCCC AGCTTGTTAC AAGAAGAACG GCCCTCTGGC TGTCACCGAT 
GCGAATCTCG CTCTCGGCAG ACTTTTGCCC GAATATTTCC CGAAGATATT TGGCCCGAAC 
GAGAACGAAC CGTTGGACAA GTCTCGAACG ATGCAAGCTT TTCGAATTCT CACCAATCAA 
ATAAACGAAT TTCTAGCGAA AGAGGGGCAC GGCGCGAAGA TGAGCATTGA GGAGGTGGCC 
ATGGGATTCG TCAGAGTCGC TAACGAAACT ATGTGTCGGC CGATACGAGC CCTCACTCAG 
GCGAAAGGTT ACGATACATC TCGCCACGTT CTGGCGTGTT TCGGGGGTGC AGGGGGGCAG 
CACGCGTGCG CCATTGCGCG TTCCTTAGGA ATGGGAACTG TATTCGTCCA CAAATATGCC 
GGAATCTTGT CAGCCTACGG AATGGCATTG GCCGACGTCG TGGAAGAGGC CCAAGAACCT 
AGCGCAGAGG TTTACGAACA GGAGTCGTTC GCACGTTTGG ACGAACGATT GGACGCCTTG 
GAGAGGAAAG TTCGCGGAAA ATTGGCTGCC CAAGGATTCC CCGAATCTCA CATAAAAACG 
GAACCTTTCC TGCATTTGCG TTATCAAGGG ACAGACTGCG CTCTAATGTG CACAGCTAGT 
CGAGACACCA AACGCAAGGG CACGAAACAC GGAGATTTTC TCAGCACCTT TTTGGAAAGG 
TACGAAACGG AGTTCGGTTT CACCATGCCC AATCGAAAGA TCCTGGTAAA CGACGTGAGG 
ATAAGGGGTA CGGGGACGAC AGATATCGAG CAAGATCCAA TTTTACCTTC GTCCAACGAG 
CCGCCACAAT TTGAAAAGAC CACCATGGTC TATTTCGAGG ACGGCTACCA GGAGACCAAA 
GTGTACCAAC TGCGCTCCCT GTCGGCCGGC CACGCGATTC GCGGACCGGC GATCATAATG 
GACAGCCTGA GCACTCTTCT GATCGAGCCC GATTGCGAGG CATCGATCAC CTCTCGCGGC 
GACGTGAGGA TAACGATCGG CCAAGGATCG AGAGCCGAGG TCACCACCGA ACTGGACACC 
ATTCAATTGA GCATATTCTC TCACCGTTTC ATGAGCATCG CCGAGCAAAT GGGCAGGATC 
CTTCAGCGTA CCGCCATCTC GACGAATATT AAGGAGCGGC TGGATTTTTC TTGCGCGGTT 
TTCGGCCCGG ACGGCGGCCT CGTTTCCAAC GCCCCCCACA TACCCGTGCA CCTGGGCGCC 
ATGCAGGAGA CGGTTCAATA CCAGATGAAG GCGTTCAAGG GGGAATTCGA GAGGGGAGAC 
GTGATCCTCG CGAATCATCC CCTGGCAGGG GGATCCCATC TTCCCGATCT TACCGTCATC 
ACTCCTGTCT TTTACGGGGA CGTTGAGAAG CCAGTGTTCT TCGTGGCCAG CAGGGGGCAT 
CATGCCGATA TTGGAGGGAT CACGCCTGGT TCCATGCCTC CGCATTCGAC TTGTCTGCTC 
CAGGAAGGGG CGACGTTTAA AAGTTTCCTG TTGGTGCACA GAGGGGTATT CAGGGAGAAA 
GAGTTGACGG AAGCGTTGAT GGAACCGGGA AAAATTGCGG GAAGTTCCGG AACCAGAAAT 
CTGTCCGATA ATCTGAGCGA TCTCAAGGCT CAGATCGCGG CAAATCACAA AGGATCGTTG 
TTGGTCAACG AGTTGATCGA TATATACGGC CTCGACGTGG TGCAAGCTTA CATGGGTCAC 
ATTCAACGCA ACGCCGAAGT TGCCGTGAGA GATATGTTGA GATCTGTCGG GAAAAGGCTG 
CTCGAGCAGA ATGGAAACAC TGTGGCAACT GCGATCGATT ACCTGGACGA TGGCAGCCCT 
ATTCAATTCC TTCTGAACAT CGACGTGGAC AAGGGTGAAG CTGTCTGCGA TTTCTCCGGC 
ACAGGGTACG AAGTGTGGGG CAATTGTAAC GCACCCAGGG CCATCACCCT TTCCGCGTTG 
ATTTATTGTT TGAGATGCAT AGTGGGCAGA GACGTTCCTT TAAATCAGGG TTGTCTGAAG 
CCTGTCAAGG TGATAATTCC AAAAGGATCG CTACTGGATC CTTCGGAGGA GGCAGCGGTG 
GTCGGTGGCA ACGTCCTCAC GTCGCAGCGC GTGGTTGACG TGGTTCTGCG AGCTTTCGGA 
GCTTGTGCAG CGTCGCAAGG GTGCATGAAC AACGTGACGC TCGGCACCGA AGAGTGGGGC 
TATTACGAGA CTGTCGCCGG GGGCAGCGGA GCCGGTCCGA CCTGGGACGG CAGGGGTGGA 
GTGCACACTC ACATGACGAA CACGAGAATC ACCGACCCCG AGATACTGGA ACTTCGTTAC 
CCGGTCATTC TCAATAAGTT TTCTCTTCGA AGCGGAAGCG GTGGAGACGG TGCCCATCGA 
GGAGGCGATG GCGTGGTCCG CGAGACGACG TTCAGAGCGC CTATGACGTT GTCCGTGTTG 
ACGGAAAGAA GGGTGAACAG CCCGCCTGGA TTGGCCGGGG GAGGATCGGG TCGAAAAGGA 
AGGAACACGT TGGTAAAAGC CGACGGTAGA AAGATCAATC TCGGCCCGAA AACAGCGGTA 
TCCGTTTATC CAGGGGACAC GTTTATACTG GAAACTCCCG GAGGCGGTGG CTACGGTCCT 
CCCGGAGTCG AGGGAGAGGG ATCGATTCGG AGCAAACCTC GAGGATTCGT GGAACGTGGG 
AGCGTGTTCG AATACAGGAA AGCTCAAGAA TCTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016767130.1
CDS	184..3822
Translation	MGTTVATNALLERKGAKMALLINEGFKDLLFIGNQARPDIFDLQVVTPEVLYEKVIEVRCRVIPASVGKCQLDNKKWRRVKGCTGEDLFITRELDEEKLRLDLEELKRSGIESLAVVLAHSYTYGEHEIRVGELAKLVGFTQVSLSHEIMPMARIVPRGFTACADAYLTPHIKQYLQGFSSGFKDRLKDVNVLFMQSDGGLTPMNSFNGSRAILSGPAGGVVGYAITTYGKETDLPVIGFDMGGTSTDVSRFGGSYEHVYESTTAGVTIQAAQLDVNTVAAGGGSMLFFRSGLFEVGPESAGAHPGPACYKKNGPLAVTDANLALGRLLPEYFPKIFGPNENEPLDKSRTMQAFRILTNQINEFLAKEGHGAKMSIEEVAMGFVRVANETMCRPIRALTQAKGYDTSRHVLACFGGAGGQHACAIARSLGMGTVFVHKYAGILSAYGMALADVVEEAQEPSAEVYEQESFARLDERLDALERKVRGKLAAQGFPESHIKTEPFLHLRYQGTDCALMCTASRDTKRKGTKHGDFLSTFLERYETEFGFTMPNRKILVNDVRIRGTGTTDIEQDPILPSSNEPPQFEKTTMVYFEDGYQETKVYQLRSLSAGHAIRGPAIIMDSLSTLLIEPDCEASITSRGDVRITIGQGSRAEVTTELDTIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCAVFGPDGGLVSNAPHIPVHLGAMQETVQYQMKAFKGEFERGDVILANHPLAGGSHLPDLTVITPVFYGDVEKPVFFVASRGHHADIGGITPGSMPPHSTCLLQEGATFKSFLLVHRGVFREKELTEALMEPGKIAGSSGTRNLSDNLSDLKAQIAANHKGSLLVNELIDIYGLDVVQAYMGHIQRNAEVAVRDMLRSVGKRLLEQNGNTVATAIDYLDDGSPIQFLLNIDVDKGEAVCDFSGTGYEVWGNCNAPRAITLSALIYCLRCIVGRDVPLNQGCLKPVKVIIPKGSLLDPSEEAAVVGGNVLTSQRVVDVVLRAFGACAASQGCMNNVTLGTEEWGYYETVAGGSGAGPTWDGRGGVHTHMTNTRITDPEILELRYPVILNKFSLRSGSGGDGAHRGGDGVVRETTFRAPMTLSVLTERRVNSPPGLAGGGSGRKGRNTLVKADGRKINLGPKTAVSVYPGDTFILETPGGGGYGPPGVEGEGSIRSKPRGFVERGSVFEYRKAQESV

Target ORF information:

RefSeq Version	XM_016911641.1
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera 5-oxoprolinase (LOC725665), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016911641.1

ORF Insert Sequence:

ATGGGAACGA CAGTCGCCAC GAACGCTCTT TTGGAAAGAA AAGGCGCGAA AATGGCGCTG 
TTGATCAACG AAGGATTCAA AGATCTTCTT TTCATCGGGA ATCAAGCACG CCCCGATATA 
TTCGACTTGC AAGTAGTCAC TCCGGAAGTT TTATACGAAA AGGTAATCGA AGTTAGGTGC 
AGAGTGATAC CCGCATCGGT TGGAAAGTGC CAGTTGGATA ACAAGAAATG GCGCAGAGTG 
AAGGGATGTA CGGGGGAGGA TCTGTTCATC ACTCGAGAAC TGGACGAGGA AAAGTTGAGA 
TTAGATCTGG AGGAATTGAA AAGATCGGGA ATCGAGAGTT TAGCCGTTGT CCTCGCGCAC 
AGTTACACAT ACGGCGAGCA CGAAATACGA GTAGGGGAAT TGGCGAAACT CGTTGGCTTC 
ACCCAAGTTT CGCTTTCGCA CGAAATCATG CCTATGGCGA GAATAGTTCC TAGAGGATTC 
ACAGCCTGCG CCGATGCATA CTTGACCCCG CACATTAAAC AATACTTACA GGGATTCTCT 
TCTGGTTTCA AAGATAGATT GAAAGATGTG AACGTGTTGT TCATGCAAAG CGACGGTGGA 
TTGACCCCGA TGAATTCATT CAATGGGTCC CGGGCCATTC TTTCCGGGCC AGCGGGCGGA 
GTCGTCGGTT ATGCGATCAC CACTTACGGC AAAGAGACCG ATTTGCCCGT GATTGGATTC 
GATATGGGCG GCACTTCTAC CGACGTCAGT CGTTTCGGTG GCAGCTACGA GCATGTGTAC 
GAGAGCACAA CCGCAGGCGT CACGATCCAA GCTGCGCAGT TGGACGTGAA CACAGTTGCG 
GCAGGGGGTG GATCGATGCT TTTCTTCAGG TCGGGTCTGT TCGAGGTCGG GCCCGAGAGC 
GCAGGGGCGC ATCCTGGCCC AGCTTGTTAC AAGAAGAACG GCCCTCTGGC TGTCACCGAT 
GCGAATCTCG CTCTCGGCAG ACTTTTGCCC GAATATTTCC CGAAGATATT TGGCCCGAAC 
GAGAACGAAC CGTTGGACAA GTCTCGAACG ATGCAAGCTT TTCGAATTCT CACCAATCAA 
ATAAACGAAT TTCTAGCGAA AGAGGGGCAC GGCGCGAAGA TGAGCATTGA GGAGGTGGCC 
ATGGGATTCG TCAGAGTCGC TAACGAAACT ATGTGTCGGC CGATACGAGC CCTCACTCAG 
GCGAAAGGTT ACGATACATC TCGCCACGTT CTGGCGTGTT TCGGGGGTGC AGGGGGGCAG 
CACGCGTGCG CCATTGCGCG TTCCTTAGGA ATGGGAACTG TATTCGTCCA CAAATATGCC 
GGAATCTTGT CAGCCTACGG AATGGCATTG GCCGACGTCG TGGAAGAGGC CCAAGAACCT 
AGCGCAGAGG TTTACGAACA GGAGTCGTTC GCACGTTTGG ACGAACGATT GGACGCCTTG 
GAGAGGAAAG TTCGCGGAAA ATTGGCTGCC CAAGGATTCC CCGAATCTCA CATAAAAACG 
GAACCTTTCC TGCATTTGCG TTATCAAGGG ACAGACTGCG CTCTAATGTG CACAGCTAGT 
CGAGACACCA AACGCAAGGG CACGAAACAC GGAGATTTTC TCAGCACCTT TTTGGAAAGG 
TACGAAACGG AGTTCGGTTT CACCATGCCC AATCGAAAGA TCCTGGTAAA CGACGTGAGG 
ATAAGGGGTA CGGGGACGAC AGATATCGAG CAAGATCCAA TTTTACCTTC GTCCAACGAG 
CCGCCACAAT TTGAAAAGAC CACCATGGTC TATTTCGAGG ACGGCTACCA GGAGACCAAA 
GTGTACCAAC TGCGCTCCCT GTCGGCCGGC CACGCGATTC GCGGACCGGC GATCATAATG 
GACAGCCTGA GCACTCTTCT GATCGAGCCC GATTGCGAGG CATCGATCAC CTCTCGCGGC 
GACGTGAGGA TAACGATCGG CCAAGGATCG AGAGCCGAGG TCACCACCGA ACTGGACACC 
ATTCAATTGA GCATATTCTC TCACCGTTTC ATGAGCATCG CCGAGCAAAT GGGCAGGATC 
CTTCAGCGTA CCGCCATCTC GACGAATATT AAGGAGCGGC TGGATTTTTC TTGCGCGGTT 
TTCGGCCCGG ACGGCGGCCT CGTTTCCAAC GCCCCCCACA TACCCGTGCA CCTGGGCGCC 
ATGCAGGAGA CGGTTCAATA CCAGATGAAG GCGTTCAAGG GGGAATTCGA GAGGGGAGAC 
GTGATCCTCG CGAATCATCC CCTGGCAGGG GGATCCCATC TTCCCGATCT TACCGTCATC 
ACTCCTGTCT TTTACGGGGA CGTTGAGAAG CCAGTGTTCT TCGTGGCCAG CAGGGGGCAT 
CATGCCGATA TTGGAGGGAT CACGCCTGGT TCCATGCCTC CGCATTCGAC TTGTCTGCTC 
CAGGAAGGGG CGACGTTTAA AAGTTTCCTG TTGGTGCACA GAGGGGTATT CAGGGAGAAA 
GAGTTGACGG AAGCGTTGAT GGAACCGGGA AAAATTGCGG GAAGTTCCGG AACCAGAAAT 
CTGTCCGATA ATCTGAGCGA TCTCAAGGCT CAGATCGCGG CAAATCACAA AGGATCGTTG 
TTGGTCAACG AGTTGATCGA TATATACGGC CTCGACGTGG TGCAAGCTTA CATGGGTCAC 
ATTCAACGCA ACGCCGAAGT TGCCGTGAGA GATATGTTGA GATCTGTCGG GAAAAGGCTG 
CTCGAGCAGA ATGGAAACAC TGTGGCAACT GCGATCGATT ACCTGGACGA TGGCAGCCCT 
ATTCAATTCC TTCTGAACAT CGACGTGGAC AAGGGTGAAG CTGTCTGCGA TTTCTCCGGC 
ACAGGGTACG AAGTGTGGGG CAATTGTAAC GCACCCAGGG CCATCACCCT TTCCGCGTTG 
ATTTATTGTT TGAGATGCAT AGTGGGCAGA GACGTTCCTT TAAATCAGGG TTGTCTGAAG 
CCTGTCAAGG TGATAATTCC AAAAGGATCG CTACTGGATC CTTCGGAGGA GGCAGCGGTG 
GTCGGTGGCA ACGTCCTCAC GTCGCAGCGC GTGGTTGACG TGGTTCTGCG AGCTTTCGGA 
GCTTGTGCAG CGTCGCAAGG GTGCATGAAC AACGTGACGC TCGGCACCGA AGAGTGGGGC 
TATTACGAGA CTGTCGCCGG GGGCAGCGGA GCCGGTCCGA CCTGGGACGG CAGGGGTGGA 
GTGCACACTC ACATGACGAA CACGAGAATC ACCGACCCCG AGATACTGGA ACTTCGTTAC 
CCGGTCATTC TCAATAAGTT TTCTCTTCGA AGCGGAAGCG GTGGAGACGG TGCCCATCGA 
GGAGGCGATG GCGTGGTCCG CGAGACGACG TTCAGAGCGC CTATGACGTT GTCCGTGTTG 
ACGGAAAGAA GGGTGAACAG CCCGCCTGGA TTGGCCGGGG GAGGATCGGG TCGAAAAGGA 
AGGAACACGT TGGTAAAAGC CGACGGTAGA AAGATCAATC TCGGCCCGAA AACAGCGGTA 
TCCGTTTATC CAGGGGACAC GTTTATACTG GAAACTCCCG GAGGCGGTGG CTACGGTCCT 
CCCGGAGTCG AGGGAGAGGG ATCGATTCGG AGCAAACCTC GAGGATTCGT GGAACGTGGG 
AGCGTGTTCG AATACAGGAA AGCTCAAGAA TCTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC725665 ( XM_006567267.3 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_006567330.1 Apis mellifera 5-oxoprolinase (LOC725665), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHd30405
Clone ID Related Accession (Same CDS sequence)	XM_006567267.3
Accession Version	XM_006567267.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-18
Organism	Apis mellifera(honey bee)
Product	5-oxoprolinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037641.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 19, 2018 this sequence version replaced XM_006567267.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGGTC GTGATAAATT CGAGTTCGCC ATAGATCGTG GCGGCACGTT TACCGACGTG 
TACGCTAGGT GTCCAGGCGG GAAAATTCGA GTGATGAAAC TGCTCTCCGT GGATCCTGCC 
AACTACGACG ACGCGCCGCG CGAAGGTATT CGCAGGATTC TCGAACAGGA GACTGGGAAG 
AGGATCGAAG GGGAGATAGA CGCGTCGTTG ATATCGTGGA TTCGAATGGG AACGACAGTC 
GCCACGAACG CTCTTTTGGA AAGAAAAGGC GCGAAAATGG CGCTGTTGAT CAACGAAGGA 
TTCAAAGATC TTCTTTTCAT CGGGAATCAA GCACGCCCCG ATATATTCGA CTTGCAAGTA 
GTCACTCCGG AAGTTTTATA CGAAAAGGTA ATCGAAGTTA GGTGCAGAGT GATACCCGCA 
TCGGTTGGAA AGTGCCAGTT GGATAACAAG AAATGGCGCA GAGTGAAGGG ATGTACGGGG 
GAGGATCTGT TCATCACTCG AGAACTGGAC GAGGAAAAGT TGAGATTAGA TCTGGAGGAA 
TTGAAAAGAT CGGGAATCGA GAGTTTAGCC GTTGTCCTCG CGCACAGTTA CACATACGGC 
GAGCACGAAA TACGAGTAGG GGAATTGGCG AAACTCGTTG GCTTCACCCA AGTTTCGCTT 
TCGCACGAAA TCATGCCTAT GGCGAGAATA GTTCCTAGAG GATTCACAGC CTGCGCCGAT 
GCATACTTGA CCCCGCACAT TAAACAATAC TTACAGGGAT TCTCTTCTGG TTTCAAAGAT 
AGATTGAAAG ATGTGAACGT GTTGTTCATG CAAAGCGACG GTGGATTGAC CCCGATGAAT 
TCATTCAATG GGTCCCGGGC CATTCTTTCC GGGCCAGCGG GCGGAGTCGT CGGTTATGCG 
ATCACCACTT ACGGCAAAGA GACCGATTTG CCCGTGATTG GATTCGATAT GGGCGGCACT 
TCTACCGACG TCAGTCGTTT CGGTGGCAGC TACGAGCATG TGTACGAGAG CACAACCGCA 
GGCGTCACGA TCCAAGCTGC GCAGTTGGAC GTGAACACAG TTGCGGCAGG GGGTGGATCG 
ATGCTTTTCT TCAGGTCGGG TCTGTTCGAG GTCGGGCCCG AGAGCGCAGG GGCGCATCCT 
GGCCCAGCTT GTTACAAGAA GAACGGCCCT CTGGCTGTCA CCGATGCGAA TCTCGCTCTC 
GGCAGACTTT TGCCCGAATA TTTCCCGAAG ATATTTGGCC CGAACGAGAA CGAACCGTTG 
GACAAGTCTC GAACGATGCA AGCTTTTCGA ATTCTCACCA ATCAAATAAA CGAATTTCTA 
GCGAAAGAGG GGCACGGCGC GAAGATGAGC ATTGAGGAGG TGGCCATGGG ATTCGTCAGA 
GTCGCTAACG AAACTATGTG TCGGCCGATA CGAGCCCTCA CTCAGGCGAA AGGTTACGAT 
ACATCTCGCC ACGTTCTGGC GTGTTTCGGG GGTGCAGGGG GGCAGCACGC GTGCGCCATT 
GCGCGTTCCT TAGGAATGGG AACTGTATTC GTCCACAAAT ATGCCGGAAT CTTGTCAGCC 
TACGGAATGG CATTGGCCGA CGTCGTGGAA GAGGCCCAAG AACCTAGCGC AGAGGTTTAC 
GAACAGGAGT CGTTCGCACG TTTGGACGAA CGATTGGACG CCTTGGAGAG GAAAGTTCGC 
GGAAAATTGG CTGCCCAAGG ATTCCCCGAA TCTCACATAA AAACGGAACC TTTCCTGCAT 
TTGCGTTATC AAGGGACAGA CTGCGCTCTA ATGTGCACAG CTAGTCGAGA CACCAAACGC 
AAGGGCACGA AACACGGAGA TTTTCTCAGC ACCTTTTTGG AAAGGTACGA AACGGAGTTC 
GGTTTCACCA TGCCCAATCG AAAGATCCTG GTAAACGACG TGAGGATAAG GGGTACGGGG 
ACGACAGATA TCGAGCAAGA TCCAATTTTA CCTTCGTCCA ACGAGCCGCC ACAATTTGAA 
AAGACCACCA TGGTCTATTT CGAGGACGGC TACCAGGAGA CCAAAGTGTA CCAACTGCGC 
TCCCTGTCGG CCGGCCACGC GATTCGCGGA CCGGCGATCA TAATGGACAG CCTGAGCACT 
CTTCTGATCG AGCCCGATTG CGAGGCATCG ATCACCTCTC GCGGCGACGT GAGGATAACG 
ATCGGCCAAG GATCGAGAGC CGAGGTCACC ACCGAACTGG ACACCATTCA ATTGAGCATA 
TTCTCTCACC GTTTCATGAG CATCGCCGAG CAAATGGGCA GGATCCTTCA GCGTACCGCC 
ATCTCGACGA ATATTAAGGA ACGGCTGGAT TTTTCTTGCG CGGTTTTCGG CCCGGACGGC 
GGCCTCGTTT CCAACGCCCC CCACATACCC GTGCACCTGG GCGCCATGCA GGAGACGGTT 
CAATACCAGA TGAAGGCGTT CAAGGGGGAA TTCGAGAGGG GAGACGTGAT CCTCGCGAAT 
CATCCCCTGG CAGGGGGATC CCATCTTCCC GATCTTACCG TCATCACTCC TGTCTTTTAC 
GGGGACGTTG AGAAGCCAGT GTTCTTCGTG GCCAGCAGGG GGCATCATGC CGATATTGGA 
GGGATCACGC CTGGTTCCAT GCCTCCGCAT TCGACTTGTC TGCTCCAGGA AGGGGCGACG 
TTTAAAAGTT TCCTGTTGGT GCACAGAGGG GTATTCAGGG AGAAAGAGTT GACGGAAGCG 
TTGATGGAAC CGGGAAAAAT TGCGGGAAGT TCCGGAACCA GAAATCTGTC CGATAATCTG 
AGCGATCTCA AGGCTCAGAT CGCGGCAAAT CACAAAGGAT CGTTGTTGGT CAACGAGTTG 
ATCGATATAT ACGGCCTCGA CGTGGTGCAA GCTTACATGG GTCACATTCA ACGCAACGCC 
GAAGTTGCCG TGAGAGATAT GTTGAGATCT GTCGGGAAAA GGCTGCTCGA GCAGAATGGA 
AACACTGTGG CAACTGCGAT CGATTACCTG GACGATGGCA GCCCTATTCA ATTCCTTCTG 
AACATCGACG TGGACAAGGG TGAAGCTGTC TGCGATTTCT CCGGCACAGG GTACGAAGTG 
TGGGGCAATT GCAACGCACC CAGGGCCATC ACCCTTTCCG CGTTGATTTA TTGTTTGAGA 
TGCATAGTGG GCAGAGACGT TCCTTTAAAT CAGGGTTGTC TGAAGCCTGT CAAGGTGATA 
ATTCCAAAAG GATCGCTACT GGATCCTTCG GAGGAGGCAG CGGTGGTCGG TGGCAACGTC 
CTCACGTCGC AGCGCGTGGT TGACGTGGTT CTGCGAGCTT TCGGAGCTTG TGCAGCGTCG 
CAAGGGTGCA TGAACAACGT GACGCTCGGC ACCGAAGAGT GGGGCTATTA CGAGACTGTC 
GCCGGGGGCA GCGGAGCCGG TCCGACCTGG GACGGCAGGG GTGGAGTGCA CACTCACATG 
ACGAACACGA GAATCACCGA CCCCGAGATA CTGGAACTTC GTTACCCGGT CATTCTCAAT 
AAGTTTTCTC TTCGAAGCGG AAGCGGTGGA GACGGTGCCC ATCGAGGAGG CGATGGCGTG 
GTCCGCGAGA CGACGTTCAG AGCGCCTATG ACGTTGTCCG TGTTGACGGA AAGAAGGGTG 
AACAGCCCGC CTGGATTGGC CGGGGGAGGA TCGGGTCGAA AAGGAAGGAA CACGTTGGTA 
AAAGCCGACG GTAGAAAGAT CAATCTCGGC CCGAAAACAG CGGTATCCGT TTATCCAGGG 
GACACGTTTA TACTGGAAAC TCCCGGAGGC GGTGGCTACG GTCCTCCCGG AGTCGAGGGA 
GAGGGATCGA TTCGGAGCAA ACCTCGAGGA TTCGTGGAAC GTGGGAGCGT GTTCGAATAC 
AGGAAAGCTC AAGAATCTGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006567330.1
CDS	733..4596
Translation	MGGRDKFEFAIDRGGTFTDVYARCPGGKIRVMKLLSVDPANYDDAPREGIRRILEQETGKRIEGEIDASLISWIRMGTTVATNALLERKGAKMALLINEGFKDLLFIGNQARPDIFDLQVVTPEVLYEKVIEVRCRVIPASVGKCQLDNKKWRRVKGCTGEDLFITRELDEEKLRLDLEELKRSGIESLAVVLAHSYTYGEHEIRVGELAKLVGFTQVSLSHEIMPMARIVPRGFTACADAYLTPHIKQYLQGFSSGFKDRLKDVNVLFMQSDGGLTPMNSFNGSRAILSGPAGGVVGYAITTYGKETDLPVIGFDMGGTSTDVSRFGGSYEHVYESTTAGVTIQAAQLDVNTVAAGGGSMLFFRSGLFEVGPESAGAHPGPACYKKNGPLAVTDANLALGRLLPEYFPKIFGPNENEPLDKSRTMQAFRILTNQINEFLAKEGHGAKMSIEEVAMGFVRVANETMCRPIRALTQAKGYDTSRHVLACFGGAGGQHACAIARSLGMGTVFVHKYAGILSAYGMALADVVEEAQEPSAEVYEQESFARLDERLDALERKVRGKLAAQGFPESHIKTEPFLHLRYQGTDCALMCTASRDTKRKGTKHGDFLSTFLERYETEFGFTMPNRKILVNDVRIRGTGTTDIEQDPILPSSNEPPQFEKTTMVYFEDGYQETKVYQLRSLSAGHAIRGPAIIMDSLSTLLIEPDCEASITSRGDVRITIGQGSRAEVTTELDTIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCAVFGPDGGLVSNAPHIPVHLGAMQETVQYQMKAFKGEFERGDVILANHPLAGGSHLPDLTVITPVFYGDVEKPVFFVASRGHHADIGGITPGSMPPHSTCLLQEGATFKSFLLVHRGVFREKELTEALMEPGKIAGSSGTRNLSDNLSDLKAQIAANHKGSLLVNELIDIYGLDVVQAYMGHIQRNAEVAVRDMLRSVGKRLLEQNGNTVATAIDYLDDGSPIQFLLNIDVDKGEAVCDFSGTGYEVWGNCNAPRAITLSALIYCLRCIVGRDVPLNQGCLKPVKVIIPKGSLLDPSEEAAVVGGNVLTSQRVVDVVLRAFGACAASQGCMNNVTLGTEEWGYYETVAGGSGAGPTWDGRGGVHTHMTNTRITDPEILELRYPVILNKFSLRSGSGGDGAHRGGDGVVRETTFRAPMTLSVLTERRVNSPPGLAGGGSGRKGRNTLVKADGRKINLGPKTAVSVYPGDTFILETPGGGGYGPPGVEGEGSIRSKPRGFVERGSVFEYRKAQESV

Target ORF information:

RefSeq Version	XM_006567267.3
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera 5-oxoprolinase (LOC725665), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006567267.3

ORF Insert Sequence:

ATGGGTGGTC GTGATAAATT CGAGTTCGCC ATAGATCGTG GCGGCACGTT TACCGACGTG 
TACGCTAGGT GTCCAGGCGG GAAAATTCGA GTGATGAAAC TGCTCTCCGT GGATCCTGCC 
AACTACGACG ACGCGCCGCG CGAAGGTATT CGCAGGATTC TCGAACAGGA GACTGGGAAG 
AGGATCGAAG GGGAGATAGA CGCGTCGTTG ATATCGTGGA TTCGAATGGG AACGACAGTC 
GCCACGAACG CTCTTTTGGA AAGAAAAGGC GCGAAAATGG CGCTGTTGAT CAACGAAGGA 
TTCAAAGATC TTCTTTTCAT CGGGAATCAA GCACGCCCCG ATATATTCGA CTTGCAAGTA 
GTCACTCCGG AAGTTTTATA CGAAAAGGTA ATCGAAGTTA GGTGCAGAGT GATACCCGCA 
TCGGTTGGAA AGTGCCAGTT GGATAACAAG AAATGGCGCA GAGTGAAGGG ATGTACGGGG 
GAGGATCTGT TCATCACTCG AGAACTGGAC GAGGAAAAGT TGAGATTAGA TCTGGAGGAA 
TTGAAAAGAT CGGGAATCGA GAGTTTAGCC GTTGTCCTCG CGCACAGTTA CACATACGGC 
GAGCACGAAA TACGAGTAGG GGAATTGGCG AAACTCGTTG GCTTCACCCA AGTTTCGCTT 
TCGCACGAAA TCATGCCTAT GGCGAGAATA GTTCCTAGAG GATTCACAGC CTGCGCCGAT 
GCATACTTGA CCCCGCACAT TAAACAATAC TTACAGGGAT TCTCTTCTGG TTTCAAAGAT 
AGATTGAAAG ATGTGAACGT GTTGTTCATG CAAAGCGACG GTGGATTGAC CCCGATGAAT 
TCATTCAATG GGTCCCGGGC CATTCTTTCC GGGCCAGCGG GCGGAGTCGT CGGTTATGCG 
ATCACCACTT ACGGCAAAGA GACCGATTTG CCCGTGATTG GATTCGATAT GGGCGGCACT 
TCTACCGACG TCAGTCGTTT CGGTGGCAGC TACGAGCATG TGTACGAGAG CACAACCGCA 
GGCGTCACGA TCCAAGCTGC GCAGTTGGAC GTGAACACAG TTGCGGCAGG GGGTGGATCG 
ATGCTTTTCT TCAGGTCGGG TCTGTTCGAG GTCGGGCCCG AGAGCGCAGG GGCGCATCCT 
GGCCCAGCTT GTTACAAGAA GAACGGCCCT CTGGCTGTCA CCGATGCGAA TCTCGCTCTC 
GGCAGACTTT TGCCCGAATA TTTCCCGAAG ATATTTGGCC CGAACGAGAA CGAACCGTTG 
GACAAGTCTC GAACGATGCA AGCTTTTCGA ATTCTCACCA ATCAAATAAA CGAATTTCTA 
GCGAAAGAGG GGCACGGCGC GAAGATGAGC ATTGAGGAGG TGGCCATGGG ATTCGTCAGA 
GTCGCTAACG AAACTATGTG TCGGCCGATA CGAGCCCTCA CTCAGGCGAA AGGTTACGAT 
ACATCTCGCC ACGTTCTGGC GTGTTTCGGG GGTGCAGGGG GGCAGCACGC GTGCGCCATT 
GCGCGTTCCT TAGGAATGGG AACTGTATTC GTCCACAAAT ATGCCGGAAT CTTGTCAGCC 
TACGGAATGG CATTGGCCGA CGTCGTGGAA GAGGCCCAAG AACCTAGCGC AGAGGTTTAC 
GAACAGGAGT CGTTCGCACG TTTGGACGAA CGATTGGACG CCTTGGAGAG GAAAGTTCGC 
GGAAAATTGG CTGCCCAAGG ATTCCCCGAA TCTCACATAA AAACGGAACC TTTCCTGCAT 
TTGCGTTATC AAGGGACAGA CTGCGCTCTA ATGTGCACAG CTAGTCGAGA CACCAAACGC 
AAGGGCACGA AACACGGAGA TTTTCTCAGC ACCTTTTTGG AAAGGTACGA AACGGAGTTC 
GGTTTCACCA TGCCCAATCG AAAGATCCTG GTAAACGACG TGAGGATAAG GGGTACGGGG 
ACGACAGATA TCGAGCAAGA TCCAATTTTA CCTTCGTCCA ACGAGCCGCC ACAATTTGAA 
AAGACCACCA TGGTCTATTT CGAGGACGGC TACCAGGAGA CCAAAGTGTA CCAACTGCGC 
TCCCTGTCGG CCGGCCACGC GATTCGCGGA CCGGCGATCA TAATGGACAG CCTGAGCACT 
CTTCTGATCG AGCCCGATTG CGAGGCATCG ATCACCTCTC GCGGCGACGT GAGGATAACG 
ATCGGCCAAG GATCGAGAGC CGAGGTCACC ACCGAACTGG ACACCATTCA ATTGAGCATA 
TTCTCTCACC GTTTCATGAG CATCGCCGAG CAAATGGGCA GGATCCTTCA GCGTACCGCC 
ATCTCGACGA ATATTAAGGA ACGGCTGGAT TTTTCTTGCG CGGTTTTCGG CCCGGACGGC 
GGCCTCGTTT CCAACGCCCC CCACATACCC GTGCACCTGG GCGCCATGCA GGAGACGGTT 
CAATACCAGA TGAAGGCGTT CAAGGGGGAA TTCGAGAGGG GAGACGTGAT CCTCGCGAAT 
CATCCCCTGG CAGGGGGATC CCATCTTCCC GATCTTACCG TCATCACTCC TGTCTTTTAC 
GGGGACGTTG AGAAGCCAGT GTTCTTCGTG GCCAGCAGGG GGCATCATGC CGATATTGGA 
GGGATCACGC CTGGTTCCAT GCCTCCGCAT TCGACTTGTC TGCTCCAGGA AGGGGCGACG 
TTTAAAAGTT TCCTGTTGGT GCACAGAGGG GTATTCAGGG AGAAAGAGTT GACGGAAGCG 
TTGATGGAAC CGGGAAAAAT TGCGGGAAGT TCCGGAACCA GAAATCTGTC CGATAATCTG 
AGCGATCTCA AGGCTCAGAT CGCGGCAAAT CACAAAGGAT CGTTGTTGGT CAACGAGTTG 
ATCGATATAT ACGGCCTCGA CGTGGTGCAA GCTTACATGG GTCACATTCA ACGCAACGCC 
GAAGTTGCCG TGAGAGATAT GTTGAGATCT GTCGGGAAAA GGCTGCTCGA GCAGAATGGA 
AACACTGTGG CAACTGCGAT CGATTACCTG GACGATGGCA GCCCTATTCA ATTCCTTCTG 
AACATCGACG TGGACAAGGG TGAAGCTGTC TGCGATTTCT CCGGCACAGG GTACGAAGTG 
TGGGGCAATT GCAACGCACC CAGGGCCATC ACCCTTTCCG CGTTGATTTA TTGTTTGAGA 
TGCATAGTGG GCAGAGACGT TCCTTTAAAT CAGGGTTGTC TGAAGCCTGT CAAGGTGATA 
ATTCCAAAAG GATCGCTACT GGATCCTTCG GAGGAGGCAG CGGTGGTCGG TGGCAACGTC 
CTCACGTCGC AGCGCGTGGT TGACGTGGTT CTGCGAGCTT TCGGAGCTTG TGCAGCGTCG 
CAAGGGTGCA TGAACAACGT GACGCTCGGC ACCGAAGAGT GGGGCTATTA CGAGACTGTC 
GCCGGGGGCA GCGGAGCCGG TCCGACCTGG GACGGCAGGG GTGGAGTGCA CACTCACATG 
ACGAACACGA GAATCACCGA CCCCGAGATA CTGGAACTTC GTTACCCGGT CATTCTCAAT 
AAGTTTTCTC TTCGAAGCGG AAGCGGTGGA GACGGTGCCC ATCGAGGAGG CGATGGCGTG 
GTCCGCGAGA CGACGTTCAG AGCGCCTATG ACGTTGTCCG TGTTGACGGA AAGAAGGGTG 
AACAGCCCGC CTGGATTGGC CGGGGGAGGA TCGGGTCGAA AAGGAAGGAA CACGTTGGTA 
AAAGCCGACG GTAGAAAGAT CAATCTCGGC CCGAAAACAG CGGTATCCGT TTATCCAGGG 
GACACGTTTA TACTGGAAAC TCCCGGAGGC GGTGGCTACG GTCCTCCCGG AGTCGAGGGA 
GAGGGATCGA TTCGGAGCAA ACCTCGAGGA TTCGTGGAAC GTGGGAGCGT GTTCGAATAC 
AGGAAAGCTC AAGAATCTGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC725665 ( XM_016911641.2 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_016767130.1 Apis mellifera 5-oxoprolinase (LOC725665), transcript variant X2, mRNA.

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CloneID	OHd30406
Clone ID Related Accession (Same CDS sequence)	XM_016911641.2
Accession Version	XM_016911641.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-18
Organism	Apis mellifera(honey bee)
Product	5-oxoprolinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037641.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 19, 2018 this sequence version replaced XM_016911641.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Apis mellifera Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAACGA CAGTCGCCAC GAACGCTCTT TTGGAAAGAA AAGGCGCGAA AATGGCGCTG 
TTGATCAACG AAGGATTCAA AGATCTTCTT TTCATCGGGA ATCAAGCACG CCCCGATATA 
TTCGACTTGC AAGTAGTCAC TCCGGAAGTT TTATACGAAA AGGTAATCGA AGTTAGGTGC 
AGAGTGATAC CCGCATCGGT TGGAAAGTGC CAGTTGGATA ACAAGAAATG GCGCAGAGTG 
AAGGGATGTA CGGGGGAGGA TCTGTTCATC ACTCGAGAAC TGGACGAGGA AAAGTTGAGA 
TTAGATCTGG AGGAATTGAA AAGATCGGGA ATCGAGAGTT TAGCCGTTGT CCTCGCGCAC 
AGTTACACAT ACGGCGAGCA CGAAATACGA GTAGGGGAAT TGGCGAAACT CGTTGGCTTC 
ACCCAAGTTT CGCTTTCGCA CGAAATCATG CCTATGGCGA GAATAGTTCC TAGAGGATTC 
ACAGCCTGCG CCGATGCATA CTTGACCCCG CACATTAAAC AATACTTACA GGGATTCTCT 
TCTGGTTTCA AAGATAGATT GAAAGATGTG AACGTGTTGT TCATGCAAAG CGACGGTGGA 
TTGACCCCGA TGAATTCATT CAATGGGTCC CGGGCCATTC TTTCCGGGCC AGCGGGCGGA 
GTCGTCGGTT ATGCGATCAC CACTTACGGC AAAGAGACCG ATTTGCCCGT GATTGGATTC 
GATATGGGCG GCACTTCTAC CGACGTCAGT CGTTTCGGTG GCAGCTACGA GCATGTGTAC 
GAGAGCACAA CCGCAGGCGT CACGATCCAA GCTGCGCAGT TGGACGTGAA CACAGTTGCG 
GCAGGGGGTG GATCGATGCT TTTCTTCAGG TCGGGTCTGT TCGAGGTCGG GCCCGAGAGC 
GCAGGGGCGC ATCCTGGCCC AGCTTGTTAC AAGAAGAACG GCCCTCTGGC TGTCACCGAT 
GCGAATCTCG CTCTCGGCAG ACTTTTGCCC GAATATTTCC CGAAGATATT TGGCCCGAAC 
GAGAACGAAC CGTTGGACAA GTCTCGAACG ATGCAAGCTT TTCGAATTCT CACCAATCAA 
ATAAACGAAT TTCTAGCGAA AGAGGGGCAC GGCGCGAAGA TGAGCATTGA GGAGGTGGCC 
ATGGGATTCG TCAGAGTCGC TAACGAAACT ATGTGTCGGC CGATACGAGC CCTCACTCAG 
GCGAAAGGTT ACGATACATC TCGCCACGTT CTGGCGTGTT TCGGGGGTGC AGGGGGGCAG 
CACGCGTGCG CCATTGCGCG TTCCTTAGGA ATGGGAACTG TATTCGTCCA CAAATATGCC 
GGAATCTTGT CAGCCTACGG AATGGCATTG GCCGACGTCG TGGAAGAGGC CCAAGAACCT 
AGCGCAGAGG TTTACGAACA GGAGTCGTTC GCACGTTTGG ACGAACGATT GGACGCCTTG 
GAGAGGAAAG TTCGCGGAAA ATTGGCTGCC CAAGGATTCC CCGAATCTCA CATAAAAACG 
GAACCTTTCC TGCATTTGCG TTATCAAGGG ACAGACTGCG CTCTAATGTG CACAGCTAGT 
CGAGACACCA AACGCAAGGG CACGAAACAC GGAGATTTTC TCAGCACCTT TTTGGAAAGG 
TACGAAACGG AGTTCGGTTT CACCATGCCC AATCGAAAGA TCCTGGTAAA CGACGTGAGG 
ATAAGGGGTA CGGGGACGAC AGATATCGAG CAAGATCCAA TTTTACCTTC GTCCAACGAG 
CCGCCACAAT TTGAAAAGAC CACCATGGTC TATTTCGAGG ACGGCTACCA GGAGACCAAA 
GTGTACCAAC TGCGCTCCCT GTCGGCCGGC CACGCGATTC GCGGACCGGC GATCATAATG 
GACAGCCTGA GCACTCTTCT GATCGAGCCC GATTGCGAGG CATCGATCAC CTCTCGCGGC 
GACGTGAGGA TAACGATCGG CCAAGGATCG AGAGCCGAGG TCACCACCGA ACTGGACACC 
ATTCAATTGA GCATATTCTC TCACCGTTTC ATGAGCATCG CCGAGCAAAT GGGCAGGATC 
CTTCAGCGTA CCGCCATCTC GACGAATATT AAGGAACGGC TGGATTTTTC TTGCGCGGTT 
TTCGGCCCGG ACGGCGGCCT CGTTTCCAAC GCCCCCCACA TACCCGTGCA CCTGGGCGCC 
ATGCAGGAGA CGGTTCAATA CCAGATGAAG GCGTTCAAGG GGGAATTCGA GAGGGGAGAC 
GTGATCCTCG CGAATCATCC CCTGGCAGGG GGATCCCATC TTCCCGATCT TACCGTCATC 
ACTCCTGTCT TTTACGGGGA CGTTGAGAAG CCAGTGTTCT TCGTGGCCAG CAGGGGGCAT 
CATGCCGATA TTGGAGGGAT CACGCCTGGT TCCATGCCTC CGCATTCGAC TTGTCTGCTC 
CAGGAAGGGG CGACGTTTAA AAGTTTCCTG TTGGTGCACA GAGGGGTATT CAGGGAGAAA 
GAGTTGACGG AAGCGTTGAT GGAACCGGGA AAAATTGCGG GAAGTTCCGG AACCAGAAAT 
CTGTCCGATA ATCTGAGCGA TCTCAAGGCT CAGATCGCGG CAAATCACAA AGGATCGTTG 
TTGGTCAACG AGTTGATCGA TATATACGGC CTCGACGTGG TGCAAGCTTA CATGGGTCAC 
ATTCAACGCA ACGCCGAAGT TGCCGTGAGA GATATGTTGA GATCTGTCGG GAAAAGGCTG 
CTCGAGCAGA ATGGAAACAC TGTGGCAACT GCGATCGATT ACCTGGACGA TGGCAGCCCT 
ATTCAATTCC TTCTGAACAT CGACGTGGAC AAGGGTGAAG CTGTCTGCGA TTTCTCCGGC 
ACAGGGTACG AAGTGTGGGG CAATTGCAAC GCACCCAGGG CCATCACCCT TTCCGCGTTG 
ATTTATTGTT TGAGATGCAT AGTGGGCAGA GACGTTCCTT TAAATCAGGG TTGTCTGAAG 
CCTGTCAAGG TGATAATTCC AAAAGGATCG CTACTGGATC CTTCGGAGGA GGCAGCGGTG 
GTCGGTGGCA ACGTCCTCAC GTCGCAGCGC GTGGTTGACG TGGTTCTGCG AGCTTTCGGA 
GCTTGTGCAG CGTCGCAAGG GTGCATGAAC AACGTGACGC TCGGCACCGA AGAGTGGGGC 
TATTACGAGA CTGTCGCCGG GGGCAGCGGA GCCGGTCCGA CCTGGGACGG CAGGGGTGGA 
GTGCACACTC ACATGACGAA CACGAGAATC ACCGACCCCG AGATACTGGA ACTTCGTTAC 
CCGGTCATTC TCAATAAGTT TTCTCTTCGA AGCGGAAGCG GTGGAGACGG TGCCCATCGA 
GGAGGCGATG GCGTGGTCCG CGAGACGACG TTCAGAGCGC CTATGACGTT GTCCGTGTTG 
ACGGAAAGAA GGGTGAACAG CCCGCCTGGA TTGGCCGGGG GAGGATCGGG TCGAAAAGGA 
AGGAACACGT TGGTAAAAGC CGACGGTAGA AAGATCAATC TCGGCCCGAA AACAGCGGTA 
TCCGTTTATC CAGGGGACAC GTTTATACTG GAAACTCCCG GAGGCGGTGG CTACGGTCCT 
CCCGGAGTCG AGGGAGAGGG ATCGATTCGG AGCAAACCTC GAGGATTCGT GGAACGTGGG 
AGCGTGTTCG AATACAGGAA AGCTCAAGAA TCTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016767130.1
CDS	209..3847
Translation	MGTTVATNALLERKGAKMALLINEGFKDLLFIGNQARPDIFDLQVVTPEVLYEKVIEVRCRVIPASVGKCQLDNKKWRRVKGCTGEDLFITRELDEEKLRLDLEELKRSGIESLAVVLAHSYTYGEHEIRVGELAKLVGFTQVSLSHEIMPMARIVPRGFTACADAYLTPHIKQYLQGFSSGFKDRLKDVNVLFMQSDGGLTPMNSFNGSRAILSGPAGGVVGYAITTYGKETDLPVIGFDMGGTSTDVSRFGGSYEHVYESTTAGVTIQAAQLDVNTVAAGGGSMLFFRSGLFEVGPESAGAHPGPACYKKNGPLAVTDANLALGRLLPEYFPKIFGPNENEPLDKSRTMQAFRILTNQINEFLAKEGHGAKMSIEEVAMGFVRVANETMCRPIRALTQAKGYDTSRHVLACFGGAGGQHACAIARSLGMGTVFVHKYAGILSAYGMALADVVEEAQEPSAEVYEQESFARLDERLDALERKVRGKLAAQGFPESHIKTEPFLHLRYQGTDCALMCTASRDTKRKGTKHGDFLSTFLERYETEFGFTMPNRKILVNDVRIRGTGTTDIEQDPILPSSNEPPQFEKTTMVYFEDGYQETKVYQLRSLSAGHAIRGPAIIMDSLSTLLIEPDCEASITSRGDVRITIGQGSRAEVTTELDTIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCAVFGPDGGLVSNAPHIPVHLGAMQETVQYQMKAFKGEFERGDVILANHPLAGGSHLPDLTVITPVFYGDVEKPVFFVASRGHHADIGGITPGSMPPHSTCLLQEGATFKSFLLVHRGVFREKELTEALMEPGKIAGSSGTRNLSDNLSDLKAQIAANHKGSLLVNELIDIYGLDVVQAYMGHIQRNAEVAVRDMLRSVGKRLLEQNGNTVATAIDYLDDGSPIQFLLNIDVDKGEAVCDFSGTGYEVWGNCNAPRAITLSALIYCLRCIVGRDVPLNQGCLKPVKVIIPKGSLLDPSEEAAVVGGNVLTSQRVVDVVLRAFGACAASQGCMNNVTLGTEEWGYYETVAGGSGAGPTWDGRGGVHTHMTNTRITDPEILELRYPVILNKFSLRSGSGGDGAHRGGDGVVRETTFRAPMTLSVLTERRVNSPPGLAGGGSGRKGRNTLVKADGRKINLGPKTAVSVYPGDTFILETPGGGGYGPPGVEGEGSIRSKPRGFVERGSVFEYRKAQESV

Target ORF information:

RefSeq Version	XM_016911641.2
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera 5-oxoprolinase (LOC725665), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016911641.2

ORF Insert Sequence:

ATGGGAACGA CAGTCGCCAC GAACGCTCTT TTGGAAAGAA AAGGCGCGAA AATGGCGCTG 
TTGATCAACG AAGGATTCAA AGATCTTCTT TTCATCGGGA ATCAAGCACG CCCCGATATA 
TTCGACTTGC AAGTAGTCAC TCCGGAAGTT TTATACGAAA AGGTAATCGA AGTTAGGTGC 
AGAGTGATAC CCGCATCGGT TGGAAAGTGC CAGTTGGATA ACAAGAAATG GCGCAGAGTG 
AAGGGATGTA CGGGGGAGGA TCTGTTCATC ACTCGAGAAC TGGACGAGGA AAAGTTGAGA 
TTAGATCTGG AGGAATTGAA AAGATCGGGA ATCGAGAGTT TAGCCGTTGT CCTCGCGCAC 
AGTTACACAT ACGGCGAGCA CGAAATACGA GTAGGGGAAT TGGCGAAACT CGTTGGCTTC 
ACCCAAGTTT CGCTTTCGCA CGAAATCATG CCTATGGCGA GAATAGTTCC TAGAGGATTC 
ACAGCCTGCG CCGATGCATA CTTGACCCCG CACATTAAAC AATACTTACA GGGATTCTCT 
TCTGGTTTCA AAGATAGATT GAAAGATGTG AACGTGTTGT TCATGCAAAG CGACGGTGGA 
TTGACCCCGA TGAATTCATT CAATGGGTCC CGGGCCATTC TTTCCGGGCC AGCGGGCGGA 
GTCGTCGGTT ATGCGATCAC CACTTACGGC AAAGAGACCG ATTTGCCCGT GATTGGATTC 
GATATGGGCG GCACTTCTAC CGACGTCAGT CGTTTCGGTG GCAGCTACGA GCATGTGTAC 
GAGAGCACAA CCGCAGGCGT CACGATCCAA GCTGCGCAGT TGGACGTGAA CACAGTTGCG 
GCAGGGGGTG GATCGATGCT TTTCTTCAGG TCGGGTCTGT TCGAGGTCGG GCCCGAGAGC 
GCAGGGGCGC ATCCTGGCCC AGCTTGTTAC AAGAAGAACG GCCCTCTGGC TGTCACCGAT 
GCGAATCTCG CTCTCGGCAG ACTTTTGCCC GAATATTTCC CGAAGATATT TGGCCCGAAC 
GAGAACGAAC CGTTGGACAA GTCTCGAACG ATGCAAGCTT TTCGAATTCT CACCAATCAA 
ATAAACGAAT TTCTAGCGAA AGAGGGGCAC GGCGCGAAGA TGAGCATTGA GGAGGTGGCC 
ATGGGATTCG TCAGAGTCGC TAACGAAACT ATGTGTCGGC CGATACGAGC CCTCACTCAG 
GCGAAAGGTT ACGATACATC TCGCCACGTT CTGGCGTGTT TCGGGGGTGC AGGGGGGCAG 
CACGCGTGCG CCATTGCGCG TTCCTTAGGA ATGGGAACTG TATTCGTCCA CAAATATGCC 
GGAATCTTGT CAGCCTACGG AATGGCATTG GCCGACGTCG TGGAAGAGGC CCAAGAACCT 
AGCGCAGAGG TTTACGAACA GGAGTCGTTC GCACGTTTGG ACGAACGATT GGACGCCTTG 
GAGAGGAAAG TTCGCGGAAA ATTGGCTGCC CAAGGATTCC CCGAATCTCA CATAAAAACG 
GAACCTTTCC TGCATTTGCG TTATCAAGGG ACAGACTGCG CTCTAATGTG CACAGCTAGT 
CGAGACACCA AACGCAAGGG CACGAAACAC GGAGATTTTC TCAGCACCTT TTTGGAAAGG 
TACGAAACGG AGTTCGGTTT CACCATGCCC AATCGAAAGA TCCTGGTAAA CGACGTGAGG 
ATAAGGGGTA CGGGGACGAC AGATATCGAG CAAGATCCAA TTTTACCTTC GTCCAACGAG 
CCGCCACAAT TTGAAAAGAC CACCATGGTC TATTTCGAGG ACGGCTACCA GGAGACCAAA 
GTGTACCAAC TGCGCTCCCT GTCGGCCGGC CACGCGATTC GCGGACCGGC GATCATAATG 
GACAGCCTGA GCACTCTTCT GATCGAGCCC GATTGCGAGG CATCGATCAC CTCTCGCGGC 
GACGTGAGGA TAACGATCGG CCAAGGATCG AGAGCCGAGG TCACCACCGA ACTGGACACC 
ATTCAATTGA GCATATTCTC TCACCGTTTC ATGAGCATCG CCGAGCAAAT GGGCAGGATC 
CTTCAGCGTA CCGCCATCTC GACGAATATT AAGGAACGGC TGGATTTTTC TTGCGCGGTT 
TTCGGCCCGG ACGGCGGCCT CGTTTCCAAC GCCCCCCACA TACCCGTGCA CCTGGGCGCC 
ATGCAGGAGA CGGTTCAATA CCAGATGAAG GCGTTCAAGG GGGAATTCGA GAGGGGAGAC 
GTGATCCTCG CGAATCATCC CCTGGCAGGG GGATCCCATC TTCCCGATCT TACCGTCATC 
ACTCCTGTCT TTTACGGGGA CGTTGAGAAG CCAGTGTTCT TCGTGGCCAG CAGGGGGCAT 
CATGCCGATA TTGGAGGGAT CACGCCTGGT TCCATGCCTC CGCATTCGAC TTGTCTGCTC 
CAGGAAGGGG CGACGTTTAA AAGTTTCCTG TTGGTGCACA GAGGGGTATT CAGGGAGAAA 
GAGTTGACGG AAGCGTTGAT GGAACCGGGA AAAATTGCGG GAAGTTCCGG AACCAGAAAT 
CTGTCCGATA ATCTGAGCGA TCTCAAGGCT CAGATCGCGG CAAATCACAA AGGATCGTTG 
TTGGTCAACG AGTTGATCGA TATATACGGC CTCGACGTGG TGCAAGCTTA CATGGGTCAC 
ATTCAACGCA ACGCCGAAGT TGCCGTGAGA GATATGTTGA GATCTGTCGG GAAAAGGCTG 
CTCGAGCAGA ATGGAAACAC TGTGGCAACT GCGATCGATT ACCTGGACGA TGGCAGCCCT 
ATTCAATTCC TTCTGAACAT CGACGTGGAC AAGGGTGAAG CTGTCTGCGA TTTCTCCGGC 
ACAGGGTACG AAGTGTGGGG CAATTGCAAC GCACCCAGGG CCATCACCCT TTCCGCGTTG 
ATTTATTGTT TGAGATGCAT AGTGGGCAGA GACGTTCCTT TAAATCAGGG TTGTCTGAAG 
CCTGTCAAGG TGATAATTCC AAAAGGATCG CTACTGGATC CTTCGGAGGA GGCAGCGGTG 
GTCGGTGGCA ACGTCCTCAC GTCGCAGCGC GTGGTTGACG TGGTTCTGCG AGCTTTCGGA 
GCTTGTGCAG CGTCGCAAGG GTGCATGAAC AACGTGACGC TCGGCACCGA AGAGTGGGGC 
TATTACGAGA CTGTCGCCGG GGGCAGCGGA GCCGGTCCGA CCTGGGACGG CAGGGGTGGA 
GTGCACACTC ACATGACGAA CACGAGAATC ACCGACCCCG AGATACTGGA ACTTCGTTAC 
CCGGTCATTC TCAATAAGTT TTCTCTTCGA AGCGGAAGCG GTGGAGACGG TGCCCATCGA 
GGAGGCGATG GCGTGGTCCG CGAGACGACG TTCAGAGCGC CTATGACGTT GTCCGTGTTG 
ACGGAAAGAA GGGTGAACAG CCCGCCTGGA TTGGCCGGGG GAGGATCGGG TCGAAAAGGA 
AGGAACACGT TGGTAAAAGC CGACGGTAGA AAGATCAATC TCGGCCCGAA AACAGCGGTA 
TCCGTTTATC CAGGGGACAC GTTTATACTG GAAACTCCCG GAGGCGGTGG CTACGGTCCT 
CCCGGAGTCG AGGGAGAGGG ATCGATTCGG AGCAAACCTC GAGGATTCGT GGAACGTGGG 
AGCGTGTTCG AATACAGGAA AGCTCAAGAA TCTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC725665 ( XM_006567267.2 ) cDNA ORF clone, Apis mellifera(honey bee) -> XP_006567330.1 Apis mellifera 5-oxoprolinase (LOC725665), transcript variant X1, mRNA.

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CloneID	OHd05681
Clone ID Related Accession (Same CDS sequence)	XM_006567267.2
Accession Version	XM_006567267.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-23
Organism	Apis mellifera(honey bee)
Product	5-oxoprolinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007073.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 24, 2016 this sequence version replaced XM_006567267.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apis mellifera Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGGTC GTGATAAATT CGAGTTCGCC ATAGATCGTG GCGGCACGTT TACCGACGTG 
TACGCTAGGT GTCCAGGCGG GAAAATTCGA GTGATGAAAC TGCTCTCCGT GGATCCTGCC 
AACTACGACG ACGCGCCGCG CGAAGGTATT CGCAGGATTC TCGAACAGGA GACTGGGAAG 
AGGATCGAAG GGGAGATAGA CGCGTCGTTG ATATCGTGGA TTCGAATGGG AACGACAGTC 
GCCACGAACG CTCTTTTGGA AAGAAAAGGC GCGAAAATGG CGCTGTTGAT CAACGAAGGA 
TTCAAAGATC TTCTTTTCAT CGGGAATCAA GCACGCCCCG ATATATTCGA CTTGCAAGTA 
GTCACTCCGG AAGTTTTATA CGAAAAGGTA ATCGAAGTTA GGTGCAGAGT GATACCCGCA 
TCGGTTGGAA AGTGCCAGTT GGATAACAAG AAATGGCGCA GAGTGAAGGG ATGTACGGGG 
GAGGATCTGT TCATCACTCG AGAACTGGAC GAGGAAAAGT TGAGATTAGA TCTGGAGGAA 
TTGAAAAGAT CGGGAATCGA GAGTTTAGCC GTTGTCCTCG CGCACAGTTA CACATACGGC 
GAGCACGAAA TACGAGTAGG GGAATTGGCG AAACTCGTTG GCTTCACCCA AGTTTCGCTT 
TCGCACGAAA TCATGCCTAT GGCGAGAATA GTTCCTAGAG GATTCACAGC CTGCGCCGAT 
GCATACTTGA CCCCGCACAT TAAACAATAC TTACAGGGAT TCTCTTCTGG TTTCAAAGAT 
AGATTGAAAG ATGTGAACGT GTTGTTCATG CAAAGCGACG GTGGATTGAC CCCGATGAAT 
TCATTCAATG GGTCCCGGGC CATTCTTTCC GGGCCAGCGG GCGGAGTCGT CGGTTATGCG 
ATCACCACTT ACGGCAAAGA GACCGATTTG CCCGTGATTG GATTCGATAT GGGCGGCACT 
TCTACCGACG TCAGTCGTTT CGGTGGCAGC TACGAGCATG TGTACGAGAG CACAACCGCA 
GGCGTCACGA TCCAAGCTGC GCAGTTGGAC GTGAACACAG TTGCGGCAGG GGGTGGATCG 
ATGCTTTTCT TCAGGTCGGG TCTGTTCGAG GTCGGGCCCG AGAGCGCAGG GGCGCATCCT 
GGCCCAGCTT GTTACAAGAA GAACGGCCCT CTGGCTGTCA CCGATGCGAA TCTCGCTCTC 
GGCAGACTTT TGCCCGAATA TTTCCCGAAG ATATTTGGCC CGAACGAGAA CGAACCGTTG 
GACAAGTCTC GAACGATGCA AGCTTTTCGA ATTCTCACCA ATCAAATAAA CGAATTTCTA 
GCGAAAGAGG GGCACGGCGC GAAGATGAGC ATTGAGGAGG TGGCCATGGG ATTCGTCAGA 
GTCGCTAACG AAACTATGTG TCGGCCGATA CGAGCCCTCA CTCAGGCGAA AGGTTACGAT 
ACATCTCGCC ACGTTCTGGC GTGTTTCGGG GGTGCAGGGG GGCAGCACGC GTGCGCCATT 
GCGCGTTCCT TAGGAATGGG AACTGTATTC GTCCACAAAT ATGCCGGAAT CTTGTCAGCC 
TACGGAATGG CATTGGCCGA CGTCGTGGAA GAGGCCCAAG AACCTAGCGC AGAGGTTTAC 
GAACAGGAGT CGTTCGCACG TTTGGACGAA CGATTGGACG CCTTGGAGAG GAAAGTTCGC 
GGAAAATTGG CTGCCCAAGG ATTCCCCGAA TCTCACATAA AAACGGAACC TTTCCTGCAT 
TTGCGTTATC AAGGGACAGA CTGCGCTCTA ATGTGCACAG CTAGTCGAGA CACCAAACGC 
AAGGGCACGA AACACGGAGA TTTTCTCAGC ACCTTTTTGG AAAGGTACGA AACGGAGTTC 
GGTTTCACCA TGCCCAATCG AAAGATCCTG GTAAACGACG TGAGGATAAG GGGTACGGGG 
ACGACAGATA TCGAGCAAGA TCCAATTTTA CCTTCGTCCA ACGAGCCGCC ACAATTTGAA 
AAGACCACCA TGGTCTATTT CGAGGACGGC TACCAGGAGA CCAAAGTGTA CCAACTGCGC 
TCCCTGTCGG CCGGCCACGC GATTCGCGGA CCGGCGATCA TAATGGACAG CCTGAGCACT 
CTTCTGATCG AGCCCGATTG CGAGGCATCG ATCACCTCTC GCGGCGACGT GAGGATAACG 
ATCGGCCAAG GATCGAGAGC CGAGGTCACC ACCGAACTGG ACACCATTCA ATTGAGCATA 
TTCTCTCACC GTTTCATGAG CATCGCCGAG CAAATGGGCA GGATCCTTCA GCGTACCGCC 
ATCTCGACGA ATATTAAGGA GCGGCTGGAT TTTTCTTGCG CGGTTTTCGG CCCGGACGGC 
GGCCTCGTTT CCAACGCCCC CCACATACCC GTGCACCTGG GCGCCATGCA GGAGACGGTT 
CAATACCAGA TGAAGGCGTT CAAGGGGGAA TTCGAGAGGG GAGACGTGAT CCTCGCGAAT 
CATCCCCTGG CAGGGGGATC CCATCTTCCC GATCTTACCG TCATCACTCC TGTCTTTTAC 
GGGGACGTTG AGAAGCCAGT GTTCTTCGTG GCCAGCAGGG GGCATCATGC CGATATTGGA 
GGGATCACGC CTGGTTCCAT GCCTCCGCAT TCGACTTGTC TGCTCCAGGA AGGGGCGACG 
TTTAAAAGTT TCCTGTTGGT GCACAGAGGG GTATTCAGGG AGAAAGAGTT GACGGAAGCG 
TTGATGGAAC CGGGAAAAAT TGCGGGAAGT TCCGGAACCA GAAATCTGTC CGATAATCTG 
AGCGATCTCA AGGCTCAGAT CGCGGCAAAT CACAAAGGAT CGTTGTTGGT CAACGAGTTG 
ATCGATATAT ACGGCCTCGA CGTGGTGCAA GCTTACATGG GTCACATTCA ACGCAACGCC 
GAAGTTGCCG TGAGAGATAT GTTGAGATCT GTCGGGAAAA GGCTGCTCGA GCAGAATGGA 
AACACTGTGG CAACTGCGAT CGATTACCTG GACGATGGCA GCCCTATTCA ATTCCTTCTG 
AACATCGACG TGGACAAGGG TGAAGCTGTC TGCGATTTCT CCGGCACAGG GTACGAAGTG 
TGGGGCAATT GTAACGCACC CAGGGCCATC ACCCTTTCCG CGTTGATTTA TTGTTTGAGA 
TGCATAGTGG GCAGAGACGT TCCTTTAAAT CAGGGTTGTC TGAAGCCTGT CAAGGTGATA 
ATTCCAAAAG GATCGCTACT GGATCCTTCG GAGGAGGCAG CGGTGGTCGG TGGCAACGTC 
CTCACGTCGC AGCGCGTGGT TGACGTGGTT CTGCGAGCTT TCGGAGCTTG TGCAGCGTCG 
CAAGGGTGCA TGAACAACGT GACGCTCGGC ACCGAAGAGT GGGGCTATTA CGAGACTGTC 
GCCGGGGGCA GCGGAGCCGG TCCGACCTGG GACGGCAGGG GTGGAGTGCA CACTCACATG 
ACGAACACGA GAATCACCGA CCCCGAGATA CTGGAACTTC GTTACCCGGT CATTCTCAAT 
AAGTTTTCTC TTCGAAGCGG AAGCGGTGGA GACGGTGCCC ATCGAGGAGG CGATGGCGTG 
GTCCGCGAGA CGACGTTCAG AGCGCCTATG ACGTTGTCCG TGTTGACGGA AAGAAGGGTG 
AACAGCCCGC CTGGATTGGC CGGGGGAGGA TCGGGTCGAA AAGGAAGGAA CACGTTGGTA 
AAAGCCGACG GTAGAAAGAT CAATCTCGGC CCGAAAACAG CGGTATCCGT TTATCCAGGG 
GACACGTTTA TACTGGAAAC TCCCGGAGGC GGTGGCTACG GTCCTCCCGG AGTCGAGGGA 
GAGGGATCGA TTCGGAGCAA ACCTCGAGGA TTCGTGGAAC GTGGGAGCGT GTTCGAATAC 
AGGAAAGCTC AAGAATCTGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006567330.1
CDS	519..4382
Translation	MGGRDKFEFAIDRGGTFTDVYARCPGGKIRVMKLLSVDPANYDDAPREGIRRILEQETGKRIEGEIDASLISWIRMGTTVATNALLERKGAKMALLINEGFKDLLFIGNQARPDIFDLQVVTPEVLYEKVIEVRCRVIPASVGKCQLDNKKWRRVKGCTGEDLFITRELDEEKLRLDLEELKRSGIESLAVVLAHSYTYGEHEIRVGELAKLVGFTQVSLSHEIMPMARIVPRGFTACADAYLTPHIKQYLQGFSSGFKDRLKDVNVLFMQSDGGLTPMNSFNGSRAILSGPAGGVVGYAITTYGKETDLPVIGFDMGGTSTDVSRFGGSYEHVYESTTAGVTIQAAQLDVNTVAAGGGSMLFFRSGLFEVGPESAGAHPGPACYKKNGPLAVTDANLALGRLLPEYFPKIFGPNENEPLDKSRTMQAFRILTNQINEFLAKEGHGAKMSIEEVAMGFVRVANETMCRPIRALTQAKGYDTSRHVLACFGGAGGQHACAIARSLGMGTVFVHKYAGILSAYGMALADVVEEAQEPSAEVYEQESFARLDERLDALERKVRGKLAAQGFPESHIKTEPFLHLRYQGTDCALMCTASRDTKRKGTKHGDFLSTFLERYETEFGFTMPNRKILVNDVRIRGTGTTDIEQDPILPSSNEPPQFEKTTMVYFEDGYQETKVYQLRSLSAGHAIRGPAIIMDSLSTLLIEPDCEASITSRGDVRITIGQGSRAEVTTELDTIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCAVFGPDGGLVSNAPHIPVHLGAMQETVQYQMKAFKGEFERGDVILANHPLAGGSHLPDLTVITPVFYGDVEKPVFFVASRGHHADIGGITPGSMPPHSTCLLQEGATFKSFLLVHRGVFREKELTEALMEPGKIAGSSGTRNLSDNLSDLKAQIAANHKGSLLVNELIDIYGLDVVQAYMGHIQRNAEVAVRDMLRSVGKRLLEQNGNTVATAIDYLDDGSPIQFLLNIDVDKGEAVCDFSGTGYEVWGNCNAPRAITLSALIYCLRCIVGRDVPLNQGCLKPVKVIIPKGSLLDPSEEAAVVGGNVLTSQRVVDVVLRAFGACAASQGCMNNVTLGTEEWGYYETVAGGSGAGPTWDGRGGVHTHMTNTRITDPEILELRYPVILNKFSLRSGSGGDGAHRGGDGVVRETTFRAPMTLSVLTERRVNSPPGLAGGGSGRKGRNTLVKADGRKINLGPKTAVSVYPGDTFILETPGGGGYGPPGVEGEGSIRSKPRGFVERGSVFEYRKAQESV

Target ORF information:

RefSeq Version	XM_006567267.2
Organism	Apis mellifera(honey bee)
Definition	Apis mellifera 5-oxoprolinase (LOC725665), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006567267.2

ORF Insert Sequence:

ATGGGTGGTC GTGATAAATT CGAGTTCGCC ATAGATCGTG GCGGCACGTT TACCGACGTG 
TACGCTAGGT GTCCAGGCGG GAAAATTCGA GTGATGAAAC TGCTCTCCGT GGATCCTGCC 
AACTACGACG ACGCGCCGCG CGAAGGTATT CGCAGGATTC TCGAACAGGA GACTGGGAAG 
AGGATCGAAG GGGAGATAGA CGCGTCGTTG ATATCGTGGA TTCGAATGGG AACGACAGTC 
GCCACGAACG CTCTTTTGGA AAGAAAAGGC GCGAAAATGG CGCTGTTGAT CAACGAAGGA 
TTCAAAGATC TTCTTTTCAT CGGGAATCAA GCACGCCCCG ATATATTCGA CTTGCAAGTA 
GTCACTCCGG AAGTTTTATA CGAAAAGGTA ATCGAAGTTA GGTGCAGAGT GATACCCGCA 
TCGGTTGGAA AGTGCCAGTT GGATAACAAG AAATGGCGCA GAGTGAAGGG ATGTACGGGG 
GAGGATCTGT TCATCACTCG AGAACTGGAC GAGGAAAAGT TGAGATTAGA TCTGGAGGAA 
TTGAAAAGAT CGGGAATCGA GAGTTTAGCC GTTGTCCTCG CGCACAGTTA CACATACGGC 
GAGCACGAAA TACGAGTAGG GGAATTGGCG AAACTCGTTG GCTTCACCCA AGTTTCGCTT 
TCGCACGAAA TCATGCCTAT GGCGAGAATA GTTCCTAGAG GATTCACAGC CTGCGCCGAT 
GCATACTTGA CCCCGCACAT TAAACAATAC TTACAGGGAT TCTCTTCTGG TTTCAAAGAT 
AGATTGAAAG ATGTGAACGT GTTGTTCATG CAAAGCGACG GTGGATTGAC CCCGATGAAT 
TCATTCAATG GGTCCCGGGC CATTCTTTCC GGGCCAGCGG GCGGAGTCGT CGGTTATGCG 
ATCACCACTT ACGGCAAAGA GACCGATTTG CCCGTGATTG GATTCGATAT GGGCGGCACT 
TCTACCGACG TCAGTCGTTT CGGTGGCAGC TACGAGCATG TGTACGAGAG CACAACCGCA 
GGCGTCACGA TCCAAGCTGC GCAGTTGGAC GTGAACACAG TTGCGGCAGG GGGTGGATCG 
ATGCTTTTCT TCAGGTCGGG TCTGTTCGAG GTCGGGCCCG AGAGCGCAGG GGCGCATCCT 
GGCCCAGCTT GTTACAAGAA GAACGGCCCT CTGGCTGTCA CCGATGCGAA TCTCGCTCTC 
GGCAGACTTT TGCCCGAATA TTTCCCGAAG ATATTTGGCC CGAACGAGAA CGAACCGTTG 
GACAAGTCTC GAACGATGCA AGCTTTTCGA ATTCTCACCA ATCAAATAAA CGAATTTCTA 
GCGAAAGAGG GGCACGGCGC GAAGATGAGC ATTGAGGAGG TGGCCATGGG ATTCGTCAGA 
GTCGCTAACG AAACTATGTG TCGGCCGATA CGAGCCCTCA CTCAGGCGAA AGGTTACGAT 
ACATCTCGCC ACGTTCTGGC GTGTTTCGGG GGTGCAGGGG GGCAGCACGC GTGCGCCATT 
GCGCGTTCCT TAGGAATGGG AACTGTATTC GTCCACAAAT ATGCCGGAAT CTTGTCAGCC 
TACGGAATGG CATTGGCCGA CGTCGTGGAA GAGGCCCAAG AACCTAGCGC AGAGGTTTAC 
GAACAGGAGT CGTTCGCACG TTTGGACGAA CGATTGGACG CCTTGGAGAG GAAAGTTCGC 
GGAAAATTGG CTGCCCAAGG ATTCCCCGAA TCTCACATAA AAACGGAACC TTTCCTGCAT 
TTGCGTTATC AAGGGACAGA CTGCGCTCTA ATGTGCACAG CTAGTCGAGA CACCAAACGC 
AAGGGCACGA AACACGGAGA TTTTCTCAGC ACCTTTTTGG AAAGGTACGA AACGGAGTTC 
GGTTTCACCA TGCCCAATCG AAAGATCCTG GTAAACGACG TGAGGATAAG GGGTACGGGG 
ACGACAGATA TCGAGCAAGA TCCAATTTTA CCTTCGTCCA ACGAGCCGCC ACAATTTGAA 
AAGACCACCA TGGTCTATTT CGAGGACGGC TACCAGGAGA CCAAAGTGTA CCAACTGCGC 
TCCCTGTCGG CCGGCCACGC GATTCGCGGA CCGGCGATCA TAATGGACAG CCTGAGCACT 
CTTCTGATCG AGCCCGATTG CGAGGCATCG ATCACCTCTC GCGGCGACGT GAGGATAACG 
ATCGGCCAAG GATCGAGAGC CGAGGTCACC ACCGAACTGG ACACCATTCA ATTGAGCATA 
TTCTCTCACC GTTTCATGAG CATCGCCGAG CAAATGGGCA GGATCCTTCA GCGTACCGCC 
ATCTCGACGA ATATTAAGGA GCGGCTGGAT TTTTCTTGCG CGGTTTTCGG CCCGGACGGC 
GGCCTCGTTT CCAACGCCCC CCACATACCC GTGCACCTGG GCGCCATGCA GGAGACGGTT 
CAATACCAGA TGAAGGCGTT CAAGGGGGAA TTCGAGAGGG GAGACGTGAT CCTCGCGAAT 
CATCCCCTGG CAGGGGGATC CCATCTTCCC GATCTTACCG TCATCACTCC TGTCTTTTAC 
GGGGACGTTG AGAAGCCAGT GTTCTTCGTG GCCAGCAGGG GGCATCATGC CGATATTGGA 
GGGATCACGC CTGGTTCCAT GCCTCCGCAT TCGACTTGTC TGCTCCAGGA AGGGGCGACG 
TTTAAAAGTT TCCTGTTGGT GCACAGAGGG GTATTCAGGG AGAAAGAGTT GACGGAAGCG 
TTGATGGAAC CGGGAAAAAT TGCGGGAAGT TCCGGAACCA GAAATCTGTC CGATAATCTG 
AGCGATCTCA AGGCTCAGAT CGCGGCAAAT CACAAAGGAT CGTTGTTGGT CAACGAGTTG 
ATCGATATAT ACGGCCTCGA CGTGGTGCAA GCTTACATGG GTCACATTCA ACGCAACGCC 
GAAGTTGCCG TGAGAGATAT GTTGAGATCT GTCGGGAAAA GGCTGCTCGA GCAGAATGGA 
AACACTGTGG CAACTGCGAT CGATTACCTG GACGATGGCA GCCCTATTCA ATTCCTTCTG 
AACATCGACG TGGACAAGGG TGAAGCTGTC TGCGATTTCT CCGGCACAGG GTACGAAGTG 
TGGGGCAATT GTAACGCACC CAGGGCCATC ACCCTTTCCG CGTTGATTTA TTGTTTGAGA 
TGCATAGTGG GCAGAGACGT TCCTTTAAAT CAGGGTTGTC TGAAGCCTGT CAAGGTGATA 
ATTCCAAAAG GATCGCTACT GGATCCTTCG GAGGAGGCAG CGGTGGTCGG TGGCAACGTC 
CTCACGTCGC AGCGCGTGGT TGACGTGGTT CTGCGAGCTT TCGGAGCTTG TGCAGCGTCG 
CAAGGGTGCA TGAACAACGT GACGCTCGGC ACCGAAGAGT GGGGCTATTA CGAGACTGTC 
GCCGGGGGCA GCGGAGCCGG TCCGACCTGG GACGGCAGGG GTGGAGTGCA CACTCACATG 
ACGAACACGA GAATCACCGA CCCCGAGATA CTGGAACTTC GTTACCCGGT CATTCTCAAT 
AAGTTTTCTC TTCGAAGCGG AAGCGGTGGA GACGGTGCCC ATCGAGGAGG CGATGGCGTG 
GTCCGCGAGA CGACGTTCAG AGCGCCTATG ACGTTGTCCG TGTTGACGGA AAGAAGGGTG 
AACAGCCCGC CTGGATTGGC CGGGGGAGGA TCGGGTCGAA AAGGAAGGAA CACGTTGGTA 
AAAGCCGACG GTAGAAAGAT CAATCTCGGC CCGAAAACAG CGGTATCCGT TTATCCAGGG 
GACACGTTTA TACTGGAAAC TCCCGGAGGC GGTGGCTACG GTCCTCCCGG AGTCGAGGGA 
GAGGGATCGA TTCGGAGCAA ACCTCGAGGA TTCGTGGAAC GTGGGAGCGT GTTCGAATAC 
AGGAAAGCTC AAGAATCTGT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC725665 cDNA ORF clone, Apis mellifera(honey bee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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