DIR1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	DIR1
Entrez Gene ID	834904
Full Name	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein
Synonyms	DEFECTIVE IN INDUCED RESISTANCE 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_124224.3	NP_568699.1	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	DIR1	NP_568699.1
Arabidopsis thaliana (thale cress)	AT5G48490	NP_199660.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

cell-to-cell movement of DIR1 through plasmodesmata is important during long-distance systemic acquired resistance signalling in Arabidopsis.
Title: Plasmodesmata-located protein overexpression negatively impacts the manifestation of systemic acquired resistance and the long-distance movement of Defective in Induced Resistance1 in Arabidopsis.

The lipid transfer proteins DIR1 which is required for G3P- and azelaic acid-induced Systemic acquired resistance, is essential for G3P accumulation.
Title: A feedback regulatory loop between G3P and lipid transfer proteins DIR1 and AZI1 mediates azelaic-acid-induced systemic immunity.

DIR1 together with a glycerol-3-phosphate-derived compound are sufficient for the induction of systemic acquired resistance in wild type plants.
Title: Glycerol-3-phosphate and systemic immunity.

DIR1 contains a signal sequence that directs it to the cell wall, and is upregulated during the basal resistance response to Pseudomonas syringae.
Title: Localization of DIR1 at the tissue, cellular and subcellular levels during Systemic Acquired Resistance in Arabidopsis using DIR1:GUS and DIR1:EGFP reporters.

The structure of DIR1 reveals a new type of lipid transfer protein.
Title: The structure of "defective in induced resistance" protein of Arabidopsis thaliana, DIR1, reveals a new type of lipid transfer protein.

The following DIR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DIR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb20929	NM_124224.3 Latest version!	Arabidopsis thaliana Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein (DIR1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DIR1 ( NM_124224.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_568699.1 Arabidopsis thaliana Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein (DIR1), mRNA.

Price & Availability↑

CloneID	OAb20929
Clone ID Related Accession (Same CDS sequence)	NM_124224.3
Accession Version	NM_124224.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_124224.2.

ATGGCGAGCA AGAAAGCAGC TATGGTTATG ATGGCGATGA TCGTGATAAT GGCTATGTTG 
GTCGATACAT CAGTAGCGAT AGATCTCTGC GGCATGAGCC AGGATGAGTT GAATGAGTGC 
AAACCAGCGG TTAGCAAGGA GAATCCGACG AGCCCATCAC AGCCTTGCTG CACCGCTCTG 
CAACACGCTG ATTTTGCATG TCTTTGTGGT TACAAGAACT CTCCATGGCT CGGTTCTTTC 
GGTGTTGATC CTGAACTCGC TTCTGCTCTC CCCAAACAGT GTGGTCTAGC CAACGCCCCA 
ACTTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_568699.1
CDS	216..524
Translation	MASKKAAMVMMAMIVIMAMLVDTSVAIDLCGMSQDELNECKPAVSKENPTSPSQPCCTALQHADFACLCGYKNSPWLGSFGVDPELASALPKQCGLANAPTC

Target ORF information:

RefSeq Version	NM_124224.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein (DIR1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_124224.3

ORF Insert Sequence:

ATGGCGAGCA AGAAAGCAGC TATGGTTATG ATGGCGATGA TCGTGATAAT GGCTATGTTG 
GTCGATACAT CAGTAGCGAT AGATCTCTGC GGCATGAGCC AGGATGAGTT GAATGAGTGC 
AAACCAGCGG TTAGCAAGGA GAATCCGACG AGCCCATCAC AGCCTTGCTG CACCGCTCTG 
CAACACGCTG ATTTTGCATG TCTTTGTGGT TACAAGAACT CTCCATGGCT CGGTTCTTTC 
GGTGTTGATC CTGAACTCGC TTCTGCTCTC CCCAAACAGT GTGGTCTAGC CAACGCCCCA 
ACTTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
Tabata S,Kaneko T,Nakamura Y,Kotani H,Kato T,Asamizu E,Miyajima N,Sasamoto S,Kimura T,Hosouchi T,Kawashima K,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Naruo K,Okumura S,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Sato S,de la Bastide M,Huang E,Spiegel L,Gnoj L,O'Shaughnessy A,Preston R,Habermann K,Murray J,Johnson D,Rohlfing T,Nelson J,Stoneking T,Pepin K,Spieth J,Sekhon M,Armstrong J,Becker M,Belter E,Cordum H,Cordes M,Courtney L,Courtney W,Dante M,Du H,Edwards J,Fryman J,Haakensen B,Lamar E,Latreille P,Leonard S,Meyer R,Mulvaney E,Ozersky P,Riley A,Strowmatt C,Wagner-McPherson C,Wollam A,Yoakum M,Bell M,Dedhia N,Parnell L,Shah R,Rodriguez M,See LH,Vil D,Baker J,Kirchoff K,Toth K,King L,Bahret A,Miller B,Marra M,Martienssen R,McCombie WR,Wilson RK,Murphy G,Bancroft I,Volckaert G,Wambutt R,D?sterh?ft A,Stiekema W,Pohl T,Entian KD,Terryn N,Hartley N,Bent E,Johnson S,Langham SA,McCullagh B,Robben J,Grymonprez B,Zimmermann W,Ramsperger U,Wedler H,Balke K,Wedler E,Peters S,van Staveren M,Dirkse W,Mooijman P,Lankhorst RK,Weitzenegger T,Bothe G,Rose M,Hauf J,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Villarroel R,Gielen J,Ardiles W,Bents O,Lemcke K,Kolesov G,Mayer K,Rudd S,Schoof H,Schueller C,Zaccaria P,Mewes HW,Bevan M,Fransz P,,,

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DIR1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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