MEE5 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	MEE5
Entrez Gene ID	837131
Full Name	Ribosomal protein S5/Elongation factor G/III/V family protein
Synonyms	CLO,CLOTHO,F9P14.8,F9P14_8,GAMETOPHYTE FACTOR 1,GFA1,MATERNAL EFFECT EMBRYO ARREST 5
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001331621.1	NP_001321282.1	Ribosomal protein S5/Elongation factor G/III/V family protein
NM_179269.4	NP_849600.1	Ribosomal protein S5/Elongation factor G/III/V family protein
NM_100503.5	NP_172112.1	Ribosomal protein S5/Elongation factor G/III/V family protein

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	EFTUD2	XP_005624439.1
Saccharomyces cerevisiae (baker's yeast)	SNU114	NP_012748.1
Eremothecium gossypii	AGOS_AEL124W	NP_984737.1
Schizosaccharomyces pombe (fission yeast)	cwf10	NP_596689.2
Xenopus tropicalis (tropical clawed frog)	eftud2	NP_001025537.1
Homo sapiens (human)	EFTUD2	NP_001245282.1
Pan troglodytes (chimpanzee)	EFTUD2	XP_001145980.1
Oryza sativa (rice)	Os06g0608300	NP_001058038.1
Rattus norvegicus (Norway rat)	Eftud2	XP_213492.3
Arabidopsis thaliana (thale cress)	MEE5	NP_172112.1
Arabidopsis thaliana (thale cress)	AT5G25230	NP_197905.1
Mus musculus (house mouse)	Eftud2	NP_001103465.1
Danio rerio (zebrafish)	eftud2	NP_956802.2
Drosophila melanogaster (fruit fly)	CG4849	NP_651605.1
	EFTUD2	XP_001114964.1
Bos taurus (cattle)	EFTUD2	NP_001076865.1
Gallus gallus (chicken)	EFTUD2	NP_001026672.1
Caenorhabditis elegans (roundworm)	eftu-2	NP_498308.1
Kluyveromyces lactis	KLLA0A06688g	XP_451298.1
Magnaporthe oryzae (rice blast fungus)	MGG_13500	XP_003716278.1
Neurospora crassa	NCU02572	XP_965703.2

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

interaction with ROOT INITIATION DEFECTIVE 1 is involved in female gametophyte development
Title: Interaction between RNA helicase ROOT INITIATION DEFECTIVE 1 and GAMETOPHYTIC FACTOR 1 is involved in female gametophyte development in Arabidopsis.

GFA1 is involved in mRNA biogenesis through interaction with AtBrr2 and AtPrp8 and functions in megagametogenesis and embryogenesis in plant. [GFA1]
Title: GAMETOPHYTIC FACTOR 1, involved in pre-mRNA splicing, is essential for megagametogenesis and embryogenesis in Arabidopsis.

VAJ/GFA1/CLO co-localized with a serine/arginine-rich (SR) protein SC35 in the nuclei and has a novel role in the directional control of floral organ growth in Arabidopsis.
Title: VAJ/GFA1/CLO is involved in the directional control of floral organ growth.

CLOTHO/GAMETOPHYTIC FACTOR 1 (CLO/GFA1) is necessary for the restricted expression of egg- and central-cell fate. CLO/GFA1 encodes the Arabidopsis homologue of Snu114, a protein that is considered to be an essential component of the spliceosome.
Title: CLO/GFA1 and ATO are novel regulators of gametic cell fate in plants.

The following MEE5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MEE5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb22828	NM_179269.4 Latest version!	Arabidopsis thaliana Ribosomal protein S5/Elongation factor G/III/V family protein (MEE5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb22828	NM_001331621.1 Latest version!	Arabidopsis thaliana Ribosomal protein S5/Elongation factor G/III/V family protein (MEE5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb22828	NM_100503.5 Latest version!	Arabidopsis thaliana Ribosomal protein S5/Elongation factor G/III/V family protein (MEE5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MEE5 ( NM_001331621.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001321282.1 Arabidopsis thaliana Ribosomal protein S5/Elongation factor G/III/V family protein (MEE5), mRNA.

Price & Availability↑

CloneID	OAb22828
Clone ID Related Accession (Same CDS sequence)	NM_001331621.1 , NM_179269.4 , NM_100503.5
Accession Version	NM_001331621.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Ribosomal protein S5/Elongation factor G/III/V family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070).

ATGGAAAGTA GCTTGTATGA TGAGTTTGGT AACTATGTTG GACCTGAGAT TGAGTCTGAC 
AGAGATAGTG ATGATGAAGT AGAAGATGAA GATCTCCAAG ATAAGCATCT TGAAGAAAAT 
GGTTCCGATG GCGAACAAGG GCCTGGGGGT TCTAATGGCT GGATTACCAC TATCAATGAT 
GTTGAGATGG AGAACCAAAT TGTTCTTCCT GAGGATAAGA AGTACTACCC TACTGCTGAG 
GAGGTTTATG GTGAGGATGT TGAGACCTTG GTTATGGATG AAGATGAGCA GCCTCTTGAG 
CAACCCATTA TCAAACCTGT TAGAGATATT AGATTTGAGG TGGGGGTCAA AGATCAAGCA 
ACGTATGTGT CAACACAGTT TCTTATCGGC CTCATGTCTA ATCCTGCGCT TGTGAGGAAT 
GTTGCTCTTG TGGGGCATCT ACAGCACGGC AAAACTGTCT TCATGGATAT GTTGGTAGAG 
CAGACGCATC ATATGTCTAC TTTTAATGCT AAAAACGAGA AGCATATGAA ATATACAGAC 
ACACGAGTCG ATGAGCAGGA GAGAAATATA TCAATCAAGG CGGTTCCAAT GTCTCTTGTC 
CTTGAGGACA GTAGATCCAA ATCATACCTG TGCAATATCA TGGATACCCC TGGACATGTC 
AATTTCTCTG ACGAAATGAC TGCTTCTTTA AGACTTGCTG ATGGTGCTGT TCTGATTGTT 
GATGCTGCTG AAGGAGTGAT GGTTAACACA GAGAGAGCTA TACGGCATGC AATCCAGGAC 
CATCTCCCTA TTGTAGTTGT TATCAATAAG GTTGACAGGC TCATAACTGA GCTCAAACTA 
CCTCCAAGGG ATGCTTACTA CAAGCTGAGG CATACAATTG AAGTCATTAA TAACCACATA 
TCTGCTGCTT CAACAACTGC TGGAGACTTA CCTCTTATAG ACCCTGCAGC TGGAAATGTA 
TGTTTTGCCA GTGGTACAGC CGGATGGTCC TTCACATTAC AATCTTTTGC TAAAATGTAT 
GCCAAGCTTC ATGGTGTGGC CATGGACGTG GATAAGTTTG CATCTCGACT TTGGGGAGAT 
GTATATTATC ACTCTGATAC TAGGGTATTC AAGAGAAGTC CTCCAGTAGG TGGTGGAGAA 
AGAGCATTTG TTCAGTTTAT TCTGGAGCCC CTATACAAGA TATACAGCCA AGTGATAGGT 
GAACATAAGA AGAGTGTAGA GACCACCCTT GCAGAATTAG GAGTGACTCT GAGTAATAGT 
GCATATAAGC TGAACGTCAG ACCTCTACTT AGGTTAGCCT GTAGTTCAGT GTTTGGTTCG 
GCATCAGGGT TCACTGATAT GTTGGTAAAG CACATTCCTT CACCCAGAGA GGCTGCAGCT 
AGGAAGGTCG ACCATTCATA TACCGGAACC AAAGATTCCC CCATTTATGA ATCAATGGTA 
GAATGTGATC CATCTGGACC TCTCATGGTT AATGTGACAA AGTTGTACCC CAAATCAGAT 
ACTAGTGTTT TTGATGTGTT TGGAAGAGTT TATAGTGGTA GGCTTCAAAC AGGGCAAAGC 
GTACGTGTAC TAGGAGAAGG GTATTCACCT GAGGATGAGG AGGACATGAC TATAAAAGAA 
GTGACGAAGC TATGGATATA TCAAGCTAGG TATAGAATAC CAGTAAGCAG TGCCCCTCCC 
GGTTCCTGGG TTTTAATTGA AGGTGTCGAT GCATCCATCA TGAAAACTGC GACTCTGTGT 
AATGCAAGCT ATGATGAAGA TGTCTACATA TTCCGGGCTC TCCAATTTAA TACCCTCCCA 
GTAGTCAAGA CTGCAACTGA GCCTTTGAAT CCTAGTGAGC TGCCTAAAAT GGTGGAAGGG 
CTTAGGAAAA TTAGCAAAAG CTATCCCCTT GCGATTACCA AAGTTGAGGA GTCTGGGGAA 
CATACTATTC TAGGCACAGG TGAGTTGTAC TTGGATTCAA TCATGAAGGA CCTCAGGGAG 
CTCTATTCAG AGGTCGAAGT AAAGGTTGCA GATCCGGTGG TCTCCTTCTG TGAGACAGTG 
GTTGAATCTT CGTCGATGAA GTGTTTTGCT GAGACACCAA ACAAAAAGAA CAAAATAACT 
ATGATTGCAG AGCCATTGGA TAGAGGACTT GCAGAGGACA TTGAGAATGG CGTTGTAAGC 
ATTGACTGGA ACAGAAAACA ACTTGGTGAC TTCTTCAGGA CGAAATATGA CTGGGATTTA 
CTTGCTGCAC GTTCCATCTG GGCTTTTGGC CCTGACAAAC AGGGGCCAAA CATATTATTG 
GATGACACCC TTCCAACTGA GGTGGACAGG AACTTGATGA TGGCAGTGAA AGACTCCATT 
GTTCAAGGGT TCCAGTGGGG TGCTCGTGAA GGTCCCCTCT GTGATGAGCC CATCAGAAAT 
GTAAAGTTCA AGATTGTTGA TGCACGGATA GCACCAGAGC CATTGCATAG AGGATCTGGT 
CAGATGATTC CAACAGCAAG ACGTGTTGCA TATTCAGCTT TTCTAATGGC AACTCCAAGG 
CTAATGGAAC CTGTCTACTA TGTGGAGATA CAAACACCAA TCGACTGTGT AACTGCAATC 
TACACAGTTT TGTCACGTAG ACGTGGGCAT GTCACATCTG ATGTTCCTCA ACCCGGTACT 
CCAGCTTATA TCGTGAAGGC CTTTCTGCCT GTGATAGAGT CCTTTGGGTT TGAGACAGAT 
CTGAGGTACC ACACTCAGGG ACAAGCCTTT TGCCTATCTG TGTTTGATCA CTGGGCCATT 
GTGCCAGGAG ACCCTCTAGA CAAAGCCATC CAGTTGCGCC CACTTGAGCC CGCTCCTATT 
CAGCATCTAG CTCGGGAATT CATGGTCAAG ACCCGCCGTA GAAAGGGAAT GAGCGAAGAC 
GTCAGCGGAA ATAAATTCTT TGATGAAGCG ATGATGGTAG AGCTTGCCCA GCAAACAGGC 
GATCTCCATC TGCAGATGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001321282.1
CDS	210..3173
Translation	MESSLYDEFGNYVGPEIESDRDSDDEVEDEDLQDKHLEENGSDGEQGPGGSNGWITTINDVEMENQIVLPEDKKYYPTAEEVYGEDVETLVMDEDEQPLEQPIIKPVRDIRFEVGVKDQATYVSTQFLIGLMSNPALVRNVALVGHLQHGKTVFMDMLVEQTHHMSTFNAKNEKHMKYTDTRVDEQERNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEGVMVNTERAIRHAIQDHLPIVVVINKVDRLITELKLPPRDAYYKLRHTIEVINNHISAASTTAGDLPLIDPAAGNVCFASGTAGWSFTLQSFAKMYAKLHGVAMDVDKFASRLWGDVYYHSDTRVFKRSPPVGGGERAFVQFILEPLYKIYSQVIGEHKKSVETTLAELGVTLSNSAYKLNVRPLLRLACSSVFGSASGFTDMLVKHIPSPREAAARKVDHSYTGTKDSPIYESMVECDPSGPLMVNVTKLYPKSDTSVFDVFGRVYSGRLQTGQSVRVLGEGYSPEDEEDMTIKEVTKLWIYQARYRIPVSSAPPGSWVLIEGVDASIMKTATLCNASYDEDVYIFRALQFNTLPVVKTATEPLNPSELPKMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKVADPVVSFCETVVESSSMKCFAETPNKKNKITMIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIWAFGPDKQGPNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRKGMSEDVSGNKFFDEAMMVELAQQTGDLHLQMI

Target ORF information:

RefSeq Version	NM_001331621.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Ribosomal protein S5/Elongation factor G/III/V family protein (MEE5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331621.1

ORF Insert Sequence:

ATGGAAAGTA GCTTGTATGA TGAGTTTGGT AACTATGTTG GACCTGAGAT TGAGTCTGAC 
AGAGATAGTG ATGATGAAGT AGAAGATGAA GATCTCCAAG ATAAGCATCT TGAAGAAAAT 
GGTTCCGATG GCGAACAAGG GCCTGGGGGT TCTAATGGCT GGATTACCAC TATCAATGAT 
GTTGAGATGG AGAACCAAAT TGTTCTTCCT GAGGATAAGA AGTACTACCC TACTGCTGAG 
GAGGTTTATG GTGAGGATGT TGAGACCTTG GTTATGGATG AAGATGAGCA GCCTCTTGAG 
CAACCCATTA TCAAACCTGT TAGAGATATT AGATTTGAGG TGGGGGTCAA AGATCAAGCA 
ACGTATGTGT CAACACAGTT TCTTATCGGC CTCATGTCTA ATCCTGCGCT TGTGAGGAAT 
GTTGCTCTTG TGGGGCATCT ACAGCACGGC AAAACTGTCT TCATGGATAT GTTGGTAGAG 
CAGACGCATC ATATGTCTAC TTTTAATGCT AAAAACGAGA AGCATATGAA ATATACAGAC 
ACACGAGTCG ATGAGCAGGA GAGAAATATA TCAATCAAGG CGGTTCCAAT GTCTCTTGTC 
CTTGAGGACA GTAGATCCAA ATCATACCTG TGCAATATCA TGGATACCCC TGGACATGTC 
AATTTCTCTG ACGAAATGAC TGCTTCTTTA AGACTTGCTG ATGGTGCTGT TCTGATTGTT 
GATGCTGCTG AAGGAGTGAT GGTTAACACA GAGAGAGCTA TACGGCATGC AATCCAGGAC 
CATCTCCCTA TTGTAGTTGT TATCAATAAG GTTGACAGGC TCATAACTGA GCTCAAACTA 
CCTCCAAGGG ATGCTTACTA CAAGCTGAGG CATACAATTG AAGTCATTAA TAACCACATA 
TCTGCTGCTT CAACAACTGC TGGAGACTTA CCTCTTATAG ACCCTGCAGC TGGAAATGTA 
TGTTTTGCCA GTGGTACAGC CGGATGGTCC TTCACATTAC AATCTTTTGC TAAAATGTAT 
GCCAAGCTTC ATGGTGTGGC CATGGACGTG GATAAGTTTG CATCTCGACT TTGGGGAGAT 
GTATATTATC ACTCTGATAC TAGGGTATTC AAGAGAAGTC CTCCAGTAGG TGGTGGAGAA 
AGAGCATTTG TTCAGTTTAT TCTGGAGCCC CTATACAAGA TATACAGCCA AGTGATAGGT 
GAACATAAGA AGAGTGTAGA GACCACCCTT GCAGAATTAG GAGTGACTCT GAGTAATAGT 
GCATATAAGC TGAACGTCAG ACCTCTACTT AGGTTAGCCT GTAGTTCAGT GTTTGGTTCG 
GCATCAGGGT TCACTGATAT GTTGGTAAAG CACATTCCTT CACCCAGAGA GGCTGCAGCT 
AGGAAGGTCG ACCATTCATA TACCGGAACC AAAGATTCCC CCATTTATGA ATCAATGGTA 
GAATGTGATC CATCTGGACC TCTCATGGTT AATGTGACAA AGTTGTACCC CAAATCAGAT 
ACTAGTGTTT TTGATGTGTT TGGAAGAGTT TATAGTGGTA GGCTTCAAAC AGGGCAAAGC 
GTACGTGTAC TAGGAGAAGG GTATTCACCT GAGGATGAGG AGGACATGAC TATAAAAGAA 
GTGACGAAGC TATGGATATA TCAAGCTAGG TATAGAATAC CAGTAAGCAG TGCCCCTCCC 
GGTTCCTGGG TTTTAATTGA AGGTGTCGAT GCATCCATCA TGAAAACTGC GACTCTGTGT 
AATGCAAGCT ATGATGAAGA TGTCTACATA TTCCGGGCTC TCCAATTTAA TACCCTCCCA 
GTAGTCAAGA CTGCAACTGA GCCTTTGAAT CCTAGTGAGC TGCCTAAAAT GGTGGAAGGG 
CTTAGGAAAA TTAGCAAAAG CTATCCCCTT GCGATTACCA AAGTTGAGGA GTCTGGGGAA 
CATACTATTC TAGGCACAGG TGAGTTGTAC TTGGATTCAA TCATGAAGGA CCTCAGGGAG 
CTCTATTCAG AGGTCGAAGT AAAGGTTGCA GATCCGGTGG TCTCCTTCTG TGAGACAGTG 
GTTGAATCTT CGTCGATGAA GTGTTTTGCT GAGACACCAA ACAAAAAGAA CAAAATAACT 
ATGATTGCAG AGCCATTGGA TAGAGGACTT GCAGAGGACA TTGAGAATGG CGTTGTAAGC 
ATTGACTGGA ACAGAAAACA ACTTGGTGAC TTCTTCAGGA CGAAATATGA CTGGGATTTA 
CTTGCTGCAC GTTCCATCTG GGCTTTTGGC CCTGACAAAC AGGGGCCAAA CATATTATTG 
GATGACACCC TTCCAACTGA GGTGGACAGG AACTTGATGA TGGCAGTGAA AGACTCCATT 
GTTCAAGGGT TCCAGTGGGG TGCTCGTGAA GGTCCCCTCT GTGATGAGCC CATCAGAAAT 
GTAAAGTTCA AGATTGTTGA TGCACGGATA GCACCAGAGC CATTGCATAG AGGATCTGGT 
CAGATGATTC CAACAGCAAG ACGTGTTGCA TATTCAGCTT TTCTAATGGC AACTCCAAGG 
CTAATGGAAC CTGTCTACTA TGTGGAGATA CAAACACCAA TCGACTGTGT AACTGCAATC 
TACACAGTTT TGTCACGTAG ACGTGGGCAT GTCACATCTG ATGTTCCTCA ACCCGGTACT 
CCAGCTTATA TCGTGAAGGC CTTTCTGCCT GTGATAGAGT CCTTTGGGTT TGAGACAGAT 
CTGAGGTACC ACACTCAGGG ACAAGCCTTT TGCCTATCTG TGTTTGATCA CTGGGCCATT 
GTGCCAGGAG ACCCTCTAGA CAAAGCCATC CAGTTGCGCC CACTTGAGCC CGCTCCTATT 
CAGCATCTAG CTCGGGAATT CATGGTCAAG ACCCGCCGTA GAAAGGGAAT GAGCGAAGAC 
GTCAGCGGAA ATAAATTCTT TGATGAAGCG ATGATGGTAG AGCTTGCCCA GCAAACAGGC 
GATCTCCATC TGCAGATGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEE5 ( NM_179269.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_849600.1 Arabidopsis thaliana Ribosomal protein S5/Elongation factor G/III/V family protein (MEE5), mRNA.

Price & Availability↑

CloneID	OAb22828
Clone ID Related Accession (Same CDS sequence)	NM_001331621.1 , NM_179269.4 , NM_100503.5
Accession Version	NM_179269.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Ribosomal protein S5/Elongation factor G/III/V family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_179269.3.

ATGGAAAGTA GCTTGTATGA TGAGTTTGGT AACTATGTTG GACCTGAGAT TGAGTCTGAC 
AGAGATAGTG ATGATGAAGT AGAAGATGAA GATCTCCAAG ATAAGCATCT TGAAGAAAAT 
GGTTCCGATG GCGAACAAGG GCCTGGGGGT TCTAATGGCT GGATTACCAC TATCAATGAT 
GTTGAGATGG AGAACCAAAT TGTTCTTCCT GAGGATAAGA AGTACTACCC TACTGCTGAG 
GAGGTTTATG GTGAGGATGT TGAGACCTTG GTTATGGATG AAGATGAGCA GCCTCTTGAG 
CAACCCATTA TCAAACCTGT TAGAGATATT AGATTTGAGG TGGGGGTCAA AGATCAAGCA 
ACGTATGTGT CAACACAGTT TCTTATCGGC CTCATGTCTA ATCCTGCGCT TGTGAGGAAT 
GTTGCTCTTG TGGGGCATCT ACAGCACGGC AAAACTGTCT TCATGGATAT GTTGGTAGAG 
CAGACGCATC ATATGTCTAC TTTTAATGCT AAAAACGAGA AGCATATGAA ATATACAGAC 
ACACGAGTCG ATGAGCAGGA GAGAAATATA TCAATCAAGG CGGTTCCAAT GTCTCTTGTC 
CTTGAGGACA GTAGATCCAA ATCATACCTG TGCAATATCA TGGATACCCC TGGACATGTC 
AATTTCTCTG ACGAAATGAC TGCTTCTTTA AGACTTGCTG ATGGTGCTGT TCTGATTGTT 
GATGCTGCTG AAGGAGTGAT GGTTAACACA GAGAGAGCTA TACGGCATGC AATCCAGGAC 
CATCTCCCTA TTGTAGTTGT TATCAATAAG GTTGACAGGC TCATAACTGA GCTCAAACTA 
CCTCCAAGGG ATGCTTACTA CAAGCTGAGG CATACAATTG AAGTCATTAA TAACCACATA 
TCTGCTGCTT CAACAACTGC TGGAGACTTA CCTCTTATAG ACCCTGCAGC TGGAAATGTA 
TGTTTTGCCA GTGGTACAGC CGGATGGTCC TTCACATTAC AATCTTTTGC TAAAATGTAT 
GCCAAGCTTC ATGGTGTGGC CATGGACGTG GATAAGTTTG CATCTCGACT TTGGGGAGAT 
GTATATTATC ACTCTGATAC TAGGGTATTC AAGAGAAGTC CTCCAGTAGG TGGTGGAGAA 
AGAGCATTTG TTCAGTTTAT TCTGGAGCCC CTATACAAGA TATACAGCCA AGTGATAGGT 
GAACATAAGA AGAGTGTAGA GACCACCCTT GCAGAATTAG GAGTGACTCT GAGTAATAGT 
GCATATAAGC TGAACGTCAG ACCTCTACTT AGGTTAGCCT GTAGTTCAGT GTTTGGTTCG 
GCATCAGGGT TCACTGATAT GTTGGTAAAG CACATTCCTT CACCCAGAGA GGCTGCAGCT 
AGGAAGGTCG ACCATTCATA TACCGGAACC AAAGATTCCC CCATTTATGA ATCAATGGTA 
GAATGTGATC CATCTGGACC TCTCATGGTT AATGTGACAA AGTTGTACCC CAAATCAGAT 
ACTAGTGTTT TTGATGTGTT TGGAAGAGTT TATAGTGGTA GGCTTCAAAC AGGGCAAAGC 
GTACGTGTAC TAGGAGAAGG GTATTCACCT GAGGATGAGG AGGACATGAC TATAAAAGAA 
GTGACGAAGC TATGGATATA TCAAGCTAGG TATAGAATAC CAGTAAGCAG TGCCCCTCCC 
GGTTCCTGGG TTTTAATTGA AGGTGTCGAT GCATCCATCA TGAAAACTGC GACTCTGTGT 
AATGCAAGCT ATGATGAAGA TGTCTACATA TTCCGGGCTC TCCAATTTAA TACCCTCCCA 
GTAGTCAAGA CTGCAACTGA GCCTTTGAAT CCTAGTGAGC TGCCTAAAAT GGTGGAAGGG 
CTTAGGAAAA TTAGCAAAAG CTATCCCCTT GCGATTACCA AAGTTGAGGA GTCTGGGGAA 
CATACTATTC TAGGCACAGG TGAGTTGTAC TTGGATTCAA TCATGAAGGA CCTCAGGGAG 
CTCTATTCAG AGGTCGAAGT AAAGGTTGCA GATCCGGTGG TCTCCTTCTG TGAGACAGTG 
GTTGAATCTT CGTCGATGAA GTGTTTTGCT GAGACACCAA ACAAAAAGAA CAAAATAACT 
ATGATTGCAG AGCCATTGGA TAGAGGACTT GCAGAGGACA TTGAGAATGG CGTTGTAAGC 
ATTGACTGGA ACAGAAAACA ACTTGGTGAC TTCTTCAGGA CGAAATATGA CTGGGATTTA 
CTTGCTGCAC GTTCCATCTG GGCTTTTGGC CCTGACAAAC AGGGGCCAAA CATATTATTG 
GATGACACCC TTCCAACTGA GGTGGACAGG AACTTGATGA TGGCAGTGAA AGACTCCATT 
GTTCAAGGGT TCCAGTGGGG TGCTCGTGAA GGTCCCCTCT GTGATGAGCC CATCAGAAAT 
GTAAAGTTCA AGATTGTTGA TGCACGGATA GCACCAGAGC CATTGCATAG AGGATCTGGT 
CAGATGATTC CAACAGCAAG ACGTGTTGCA TATTCAGCTT TTCTAATGGC AACTCCAAGG 
CTAATGGAAC CTGTCTACTA TGTGGAGATA CAAACACCAA TCGACTGTGT AACTGCAATC 
TACACAGTTT TGTCACGTAG ACGTGGGCAT GTCACATCTG ATGTTCCTCA ACCCGGTACT 
CCAGCTTATA TCGTGAAGGC CTTTCTGCCT GTGATAGAGT CCTTTGGGTT TGAGACAGAT 
CTGAGGTACC ACACTCAGGG ACAAGCCTTT TGCCTATCTG TGTTTGATCA CTGGGCCATT 
GTGCCAGGAG ACCCTCTAGA CAAAGCCATC CAGTTGCGCC CACTTGAGCC CGCTCCTATT 
CAGCATCTAG CTCGGGAATT CATGGTCAAG ACCCGCCGTA GAAAGGGAAT GAGCGAAGAC 
GTCAGCGGAA ATAAATTCTT TGATGAAGCG ATGATGGTAG AGCTTGCCCA GCAAACAGGC 
GATCTCCATC TGCAGATGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_849600.1
CDS	303..3266
Translation	MESSLYDEFGNYVGPEIESDRDSDDEVEDEDLQDKHLEENGSDGEQGPGGSNGWITTINDVEMENQIVLPEDKKYYPTAEEVYGEDVETLVMDEDEQPLEQPIIKPVRDIRFEVGVKDQATYVSTQFLIGLMSNPALVRNVALVGHLQHGKTVFMDMLVEQTHHMSTFNAKNEKHMKYTDTRVDEQERNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEGVMVNTERAIRHAIQDHLPIVVVINKVDRLITELKLPPRDAYYKLRHTIEVINNHISAASTTAGDLPLIDPAAGNVCFASGTAGWSFTLQSFAKMYAKLHGVAMDVDKFASRLWGDVYYHSDTRVFKRSPPVGGGERAFVQFILEPLYKIYSQVIGEHKKSVETTLAELGVTLSNSAYKLNVRPLLRLACSSVFGSASGFTDMLVKHIPSPREAAARKVDHSYTGTKDSPIYESMVECDPSGPLMVNVTKLYPKSDTSVFDVFGRVYSGRLQTGQSVRVLGEGYSPEDEEDMTIKEVTKLWIYQARYRIPVSSAPPGSWVLIEGVDASIMKTATLCNASYDEDVYIFRALQFNTLPVVKTATEPLNPSELPKMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKVADPVVSFCETVVESSSMKCFAETPNKKNKITMIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIWAFGPDKQGPNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRKGMSEDVSGNKFFDEAMMVELAQQTGDLHLQMI

Target ORF information:

RefSeq Version	NM_179269.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Ribosomal protein S5/Elongation factor G/III/V family protein (MEE5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_179269.4

ORF Insert Sequence:

ATGGAAAGTA GCTTGTATGA TGAGTTTGGT AACTATGTTG GACCTGAGAT TGAGTCTGAC 
AGAGATAGTG ATGATGAAGT AGAAGATGAA GATCTCCAAG ATAAGCATCT TGAAGAAAAT 
GGTTCCGATG GCGAACAAGG GCCTGGGGGT TCTAATGGCT GGATTACCAC TATCAATGAT 
GTTGAGATGG AGAACCAAAT TGTTCTTCCT GAGGATAAGA AGTACTACCC TACTGCTGAG 
GAGGTTTATG GTGAGGATGT TGAGACCTTG GTTATGGATG AAGATGAGCA GCCTCTTGAG 
CAACCCATTA TCAAACCTGT TAGAGATATT AGATTTGAGG TGGGGGTCAA AGATCAAGCA 
ACGTATGTGT CAACACAGTT TCTTATCGGC CTCATGTCTA ATCCTGCGCT TGTGAGGAAT 
GTTGCTCTTG TGGGGCATCT ACAGCACGGC AAAACTGTCT TCATGGATAT GTTGGTAGAG 
CAGACGCATC ATATGTCTAC TTTTAATGCT AAAAACGAGA AGCATATGAA ATATACAGAC 
ACACGAGTCG ATGAGCAGGA GAGAAATATA TCAATCAAGG CGGTTCCAAT GTCTCTTGTC 
CTTGAGGACA GTAGATCCAA ATCATACCTG TGCAATATCA TGGATACCCC TGGACATGTC 
AATTTCTCTG ACGAAATGAC TGCTTCTTTA AGACTTGCTG ATGGTGCTGT TCTGATTGTT 
GATGCTGCTG AAGGAGTGAT GGTTAACACA GAGAGAGCTA TACGGCATGC AATCCAGGAC 
CATCTCCCTA TTGTAGTTGT TATCAATAAG GTTGACAGGC TCATAACTGA GCTCAAACTA 
CCTCCAAGGG ATGCTTACTA CAAGCTGAGG CATACAATTG AAGTCATTAA TAACCACATA 
TCTGCTGCTT CAACAACTGC TGGAGACTTA CCTCTTATAG ACCCTGCAGC TGGAAATGTA 
TGTTTTGCCA GTGGTACAGC CGGATGGTCC TTCACATTAC AATCTTTTGC TAAAATGTAT 
GCCAAGCTTC ATGGTGTGGC CATGGACGTG GATAAGTTTG CATCTCGACT TTGGGGAGAT 
GTATATTATC ACTCTGATAC TAGGGTATTC AAGAGAAGTC CTCCAGTAGG TGGTGGAGAA 
AGAGCATTTG TTCAGTTTAT TCTGGAGCCC CTATACAAGA TATACAGCCA AGTGATAGGT 
GAACATAAGA AGAGTGTAGA GACCACCCTT GCAGAATTAG GAGTGACTCT GAGTAATAGT 
GCATATAAGC TGAACGTCAG ACCTCTACTT AGGTTAGCCT GTAGTTCAGT GTTTGGTTCG 
GCATCAGGGT TCACTGATAT GTTGGTAAAG CACATTCCTT CACCCAGAGA GGCTGCAGCT 
AGGAAGGTCG ACCATTCATA TACCGGAACC AAAGATTCCC CCATTTATGA ATCAATGGTA 
GAATGTGATC CATCTGGACC TCTCATGGTT AATGTGACAA AGTTGTACCC CAAATCAGAT 
ACTAGTGTTT TTGATGTGTT TGGAAGAGTT TATAGTGGTA GGCTTCAAAC AGGGCAAAGC 
GTACGTGTAC TAGGAGAAGG GTATTCACCT GAGGATGAGG AGGACATGAC TATAAAAGAA 
GTGACGAAGC TATGGATATA TCAAGCTAGG TATAGAATAC CAGTAAGCAG TGCCCCTCCC 
GGTTCCTGGG TTTTAATTGA AGGTGTCGAT GCATCCATCA TGAAAACTGC GACTCTGTGT 
AATGCAAGCT ATGATGAAGA TGTCTACATA TTCCGGGCTC TCCAATTTAA TACCCTCCCA 
GTAGTCAAGA CTGCAACTGA GCCTTTGAAT CCTAGTGAGC TGCCTAAAAT GGTGGAAGGG 
CTTAGGAAAA TTAGCAAAAG CTATCCCCTT GCGATTACCA AAGTTGAGGA GTCTGGGGAA 
CATACTATTC TAGGCACAGG TGAGTTGTAC TTGGATTCAA TCATGAAGGA CCTCAGGGAG 
CTCTATTCAG AGGTCGAAGT AAAGGTTGCA GATCCGGTGG TCTCCTTCTG TGAGACAGTG 
GTTGAATCTT CGTCGATGAA GTGTTTTGCT GAGACACCAA ACAAAAAGAA CAAAATAACT 
ATGATTGCAG AGCCATTGGA TAGAGGACTT GCAGAGGACA TTGAGAATGG CGTTGTAAGC 
ATTGACTGGA ACAGAAAACA ACTTGGTGAC TTCTTCAGGA CGAAATATGA CTGGGATTTA 
CTTGCTGCAC GTTCCATCTG GGCTTTTGGC CCTGACAAAC AGGGGCCAAA CATATTATTG 
GATGACACCC TTCCAACTGA GGTGGACAGG AACTTGATGA TGGCAGTGAA AGACTCCATT 
GTTCAAGGGT TCCAGTGGGG TGCTCGTGAA GGTCCCCTCT GTGATGAGCC CATCAGAAAT 
GTAAAGTTCA AGATTGTTGA TGCACGGATA GCACCAGAGC CATTGCATAG AGGATCTGGT 
CAGATGATTC CAACAGCAAG ACGTGTTGCA TATTCAGCTT TTCTAATGGC AACTCCAAGG 
CTAATGGAAC CTGTCTACTA TGTGGAGATA CAAACACCAA TCGACTGTGT AACTGCAATC 
TACACAGTTT TGTCACGTAG ACGTGGGCAT GTCACATCTG ATGTTCCTCA ACCCGGTACT 
CCAGCTTATA TCGTGAAGGC CTTTCTGCCT GTGATAGAGT CCTTTGGGTT TGAGACAGAT 
CTGAGGTACC ACACTCAGGG ACAAGCCTTT TGCCTATCTG TGTTTGATCA CTGGGCCATT 
GTGCCAGGAG ACCCTCTAGA CAAAGCCATC CAGTTGCGCC CACTTGAGCC CGCTCCTATT 
CAGCATCTAG CTCGGGAATT CATGGTCAAG ACCCGCCGTA GAAAGGGAAT GAGCGAAGAC 
GTCAGCGGAA ATAAATTCTT TGATGAAGCG ATGATGGTAG AGCTTGCCCA GCAAACAGGC 
GATCTCCATC TGCAGATGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEE5 ( NM_100503.5 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_172112.1 Arabidopsis thaliana Ribosomal protein S5/Elongation factor G/III/V family protein (MEE5), mRNA.

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CloneID	OAb22828
Clone ID Related Accession (Same CDS sequence)	NM_001331621.1 , NM_179269.4 , NM_100503.5
Accession Version	NM_100503.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	Ribosomal protein S5/Elongation factor G/III/V family protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_100503.4.

ATGGAAAGTA GCTTGTATGA TGAGTTTGGT AACTATGTTG GACCTGAGAT TGAGTCTGAC 
AGAGATAGTG ATGATGAAGT AGAAGATGAA GATCTCCAAG ATAAGCATCT TGAAGAAAAT 
GGTTCCGATG GCGAACAAGG GCCTGGGGGT TCTAATGGCT GGATTACCAC TATCAATGAT 
GTTGAGATGG AGAACCAAAT TGTTCTTCCT GAGGATAAGA AGTACTACCC TACTGCTGAG 
GAGGTTTATG GTGAGGATGT TGAGACCTTG GTTATGGATG AAGATGAGCA GCCTCTTGAG 
CAACCCATTA TCAAACCTGT TAGAGATATT AGATTTGAGG TGGGGGTCAA AGATCAAGCA 
ACGTATGTGT CAACACAGTT TCTTATCGGC CTCATGTCTA ATCCTGCGCT TGTGAGGAAT 
GTTGCTCTTG TGGGGCATCT ACAGCACGGC AAAACTGTCT TCATGGATAT GTTGGTAGAG 
CAGACGCATC ATATGTCTAC TTTTAATGCT AAAAACGAGA AGCATATGAA ATATACAGAC 
ACACGAGTCG ATGAGCAGGA GAGAAATATA TCAATCAAGG CGGTTCCAAT GTCTCTTGTC 
CTTGAGGACA GTAGATCCAA ATCATACCTG TGCAATATCA TGGATACCCC TGGACATGTC 
AATTTCTCTG ACGAAATGAC TGCTTCTTTA AGACTTGCTG ATGGTGCTGT TCTGATTGTT 
GATGCTGCTG AAGGAGTGAT GGTTAACACA GAGAGAGCTA TACGGCATGC AATCCAGGAC 
CATCTCCCTA TTGTAGTTGT TATCAATAAG GTTGACAGGC TCATAACTGA GCTCAAACTA 
CCTCCAAGGG ATGCTTACTA CAAGCTGAGG CATACAATTG AAGTCATTAA TAACCACATA 
TCTGCTGCTT CAACAACTGC TGGAGACTTA CCTCTTATAG ACCCTGCAGC TGGAAATGTA 
TGTTTTGCCA GTGGTACAGC CGGATGGTCC TTCACATTAC AATCTTTTGC TAAAATGTAT 
GCCAAGCTTC ATGGTGTGGC CATGGACGTG GATAAGTTTG CATCTCGACT TTGGGGAGAT 
GTATATTATC ACTCTGATAC TAGGGTATTC AAGAGAAGTC CTCCAGTAGG TGGTGGAGAA 
AGAGCATTTG TTCAGTTTAT TCTGGAGCCC CTATACAAGA TATACAGCCA AGTGATAGGT 
GAACATAAGA AGAGTGTAGA GACCACCCTT GCAGAATTAG GAGTGACTCT GAGTAATAGT 
GCATATAAGC TGAACGTCAG ACCTCTACTT AGGTTAGCCT GTAGTTCAGT GTTTGGTTCG 
GCATCAGGGT TCACTGATAT GTTGGTAAAG CACATTCCTT CACCCAGAGA GGCTGCAGCT 
AGGAAGGTCG ACCATTCATA TACCGGAACC AAAGATTCCC CCATTTATGA ATCAATGGTA 
GAATGTGATC CATCTGGACC TCTCATGGTT AATGTGACAA AGTTGTACCC CAAATCAGAT 
ACTAGTGTTT TTGATGTGTT TGGAAGAGTT TATAGTGGTA GGCTTCAAAC AGGGCAAAGC 
GTACGTGTAC TAGGAGAAGG GTATTCACCT GAGGATGAGG AGGACATGAC TATAAAAGAA 
GTGACGAAGC TATGGATATA TCAAGCTAGG TATAGAATAC CAGTAAGCAG TGCCCCTCCC 
GGTTCCTGGG TTTTAATTGA AGGTGTCGAT GCATCCATCA TGAAAACTGC GACTCTGTGT 
AATGCAAGCT ATGATGAAGA TGTCTACATA TTCCGGGCTC TCCAATTTAA TACCCTCCCA 
GTAGTCAAGA CTGCAACTGA GCCTTTGAAT CCTAGTGAGC TGCCTAAAAT GGTGGAAGGG 
CTTAGGAAAA TTAGCAAAAG CTATCCCCTT GCGATTACCA AAGTTGAGGA GTCTGGGGAA 
CATACTATTC TAGGCACAGG TGAGTTGTAC TTGGATTCAA TCATGAAGGA CCTCAGGGAG 
CTCTATTCAG AGGTCGAAGT AAAGGTTGCA GATCCGGTGG TCTCCTTCTG TGAGACAGTG 
GTTGAATCTT CGTCGATGAA GTGTTTTGCT GAGACACCAA ACAAAAAGAA CAAAATAACT 
ATGATTGCAG AGCCATTGGA TAGAGGACTT GCAGAGGACA TTGAGAATGG CGTTGTAAGC 
ATTGACTGGA ACAGAAAACA ACTTGGTGAC TTCTTCAGGA CGAAATATGA CTGGGATTTA 
CTTGCTGCAC GTTCCATCTG GGCTTTTGGC CCTGACAAAC AGGGGCCAAA CATATTATTG 
GATGACACCC TTCCAACTGA GGTGGACAGG AACTTGATGA TGGCAGTGAA AGACTCCATT 
GTTCAAGGGT TCCAGTGGGG TGCTCGTGAA GGTCCCCTCT GTGATGAGCC CATCAGAAAT 
GTAAAGTTCA AGATTGTTGA TGCACGGATA GCACCAGAGC CATTGCATAG AGGATCTGGT 
CAGATGATTC CAACAGCAAG ACGTGTTGCA TATTCAGCTT TTCTAATGGC AACTCCAAGG 
CTAATGGAAC CTGTCTACTA TGTGGAGATA CAAACACCAA TCGACTGTGT AACTGCAATC 
TACACAGTTT TGTCACGTAG ACGTGGGCAT GTCACATCTG ATGTTCCTCA ACCCGGTACT 
CCAGCTTATA TCGTGAAGGC CTTTCTGCCT GTGATAGAGT CCTTTGGGTT TGAGACAGAT 
CTGAGGTACC ACACTCAGGG ACAAGCCTTT TGCCTATCTG TGTTTGATCA CTGGGCCATT 
GTGCCAGGAG ACCCTCTAGA CAAAGCCATC CAGTTGCGCC CACTTGAGCC CGCTCCTATT 
CAGCATCTAG CTCGGGAATT CATGGTCAAG ACCCGCCGTA GAAAGGGAAT GAGCGAAGAC 
GTCAGCGGAA ATAAATTCTT TGATGAAGCG ATGATGGTAG AGCTTGCCCA GCAAACAGGC 
GATCTCCATC TGCAGATGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_172112.1
CDS	170..3133
Translation	MESSLYDEFGNYVGPEIESDRDSDDEVEDEDLQDKHLEENGSDGEQGPGGSNGWITTINDVEMENQIVLPEDKKYYPTAEEVYGEDVETLVMDEDEQPLEQPIIKPVRDIRFEVGVKDQATYVSTQFLIGLMSNPALVRNVALVGHLQHGKTVFMDMLVEQTHHMSTFNAKNEKHMKYTDTRVDEQERNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEGVMVNTERAIRHAIQDHLPIVVVINKVDRLITELKLPPRDAYYKLRHTIEVINNHISAASTTAGDLPLIDPAAGNVCFASGTAGWSFTLQSFAKMYAKLHGVAMDVDKFASRLWGDVYYHSDTRVFKRSPPVGGGERAFVQFILEPLYKIYSQVIGEHKKSVETTLAELGVTLSNSAYKLNVRPLLRLACSSVFGSASGFTDMLVKHIPSPREAAARKVDHSYTGTKDSPIYESMVECDPSGPLMVNVTKLYPKSDTSVFDVFGRVYSGRLQTGQSVRVLGEGYSPEDEEDMTIKEVTKLWIYQARYRIPVSSAPPGSWVLIEGVDASIMKTATLCNASYDEDVYIFRALQFNTLPVVKTATEPLNPSELPKMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKVADPVVSFCETVVESSSMKCFAETPNKKNKITMIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIWAFGPDKQGPNILLDDTLPTEVDRNLMMAVKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARIAPEPLHRGSGQMIPTARRVAYSAFLMATPRLMEPVYYVEIQTPIDCVTAIYTVLSRRRGHVTSDVPQPGTPAYIVKAFLPVIESFGFETDLRYHTQGQAFCLSVFDHWAIVPGDPLDKAIQLRPLEPAPIQHLAREFMVKTRRRKGMSEDVSGNKFFDEAMMVELAQQTGDLHLQMI

Target ORF information:

RefSeq Version	NM_100503.5
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana Ribosomal protein S5/Elongation factor G/III/V family protein (MEE5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_100503.5

ORF Insert Sequence:

ATGGAAAGTA GCTTGTATGA TGAGTTTGGT AACTATGTTG GACCTGAGAT TGAGTCTGAC 
AGAGATAGTG ATGATGAAGT AGAAGATGAA GATCTCCAAG ATAAGCATCT TGAAGAAAAT 
GGTTCCGATG GCGAACAAGG GCCTGGGGGT TCTAATGGCT GGATTACCAC TATCAATGAT 
GTTGAGATGG AGAACCAAAT TGTTCTTCCT GAGGATAAGA AGTACTACCC TACTGCTGAG 
GAGGTTTATG GTGAGGATGT TGAGACCTTG GTTATGGATG AAGATGAGCA GCCTCTTGAG 
CAACCCATTA TCAAACCTGT TAGAGATATT AGATTTGAGG TGGGGGTCAA AGATCAAGCA 
ACGTATGTGT CAACACAGTT TCTTATCGGC CTCATGTCTA ATCCTGCGCT TGTGAGGAAT 
GTTGCTCTTG TGGGGCATCT ACAGCACGGC AAAACTGTCT TCATGGATAT GTTGGTAGAG 
CAGACGCATC ATATGTCTAC TTTTAATGCT AAAAACGAGA AGCATATGAA ATATACAGAC 
ACACGAGTCG ATGAGCAGGA GAGAAATATA TCAATCAAGG CGGTTCCAAT GTCTCTTGTC 
CTTGAGGACA GTAGATCCAA ATCATACCTG TGCAATATCA TGGATACCCC TGGACATGTC 
AATTTCTCTG ACGAAATGAC TGCTTCTTTA AGACTTGCTG ATGGTGCTGT TCTGATTGTT 
GATGCTGCTG AAGGAGTGAT GGTTAACACA GAGAGAGCTA TACGGCATGC AATCCAGGAC 
CATCTCCCTA TTGTAGTTGT TATCAATAAG GTTGACAGGC TCATAACTGA GCTCAAACTA 
CCTCCAAGGG ATGCTTACTA CAAGCTGAGG CATACAATTG AAGTCATTAA TAACCACATA 
TCTGCTGCTT CAACAACTGC TGGAGACTTA CCTCTTATAG ACCCTGCAGC TGGAAATGTA 
TGTTTTGCCA GTGGTACAGC CGGATGGTCC TTCACATTAC AATCTTTTGC TAAAATGTAT 
GCCAAGCTTC ATGGTGTGGC CATGGACGTG GATAAGTTTG CATCTCGACT TTGGGGAGAT 
GTATATTATC ACTCTGATAC TAGGGTATTC AAGAGAAGTC CTCCAGTAGG TGGTGGAGAA 
AGAGCATTTG TTCAGTTTAT TCTGGAGCCC CTATACAAGA TATACAGCCA AGTGATAGGT 
GAACATAAGA AGAGTGTAGA GACCACCCTT GCAGAATTAG GAGTGACTCT GAGTAATAGT 
GCATATAAGC TGAACGTCAG ACCTCTACTT AGGTTAGCCT GTAGTTCAGT GTTTGGTTCG 
GCATCAGGGT TCACTGATAT GTTGGTAAAG CACATTCCTT CACCCAGAGA GGCTGCAGCT 
AGGAAGGTCG ACCATTCATA TACCGGAACC AAAGATTCCC CCATTTATGA ATCAATGGTA 
GAATGTGATC CATCTGGACC TCTCATGGTT AATGTGACAA AGTTGTACCC CAAATCAGAT 
ACTAGTGTTT TTGATGTGTT TGGAAGAGTT TATAGTGGTA GGCTTCAAAC AGGGCAAAGC 
GTACGTGTAC TAGGAGAAGG GTATTCACCT GAGGATGAGG AGGACATGAC TATAAAAGAA 
GTGACGAAGC TATGGATATA TCAAGCTAGG TATAGAATAC CAGTAAGCAG TGCCCCTCCC 
GGTTCCTGGG TTTTAATTGA AGGTGTCGAT GCATCCATCA TGAAAACTGC GACTCTGTGT 
AATGCAAGCT ATGATGAAGA TGTCTACATA TTCCGGGCTC TCCAATTTAA TACCCTCCCA 
GTAGTCAAGA CTGCAACTGA GCCTTTGAAT CCTAGTGAGC TGCCTAAAAT GGTGGAAGGG 
CTTAGGAAAA TTAGCAAAAG CTATCCCCTT GCGATTACCA AAGTTGAGGA GTCTGGGGAA 
CATACTATTC TAGGCACAGG TGAGTTGTAC TTGGATTCAA TCATGAAGGA CCTCAGGGAG 
CTCTATTCAG AGGTCGAAGT AAAGGTTGCA GATCCGGTGG TCTCCTTCTG TGAGACAGTG 
GTTGAATCTT CGTCGATGAA GTGTTTTGCT GAGACACCAA ACAAAAAGAA CAAAATAACT 
ATGATTGCAG AGCCATTGGA TAGAGGACTT GCAGAGGACA TTGAGAATGG CGTTGTAAGC 
ATTGACTGGA ACAGAAAACA ACTTGGTGAC TTCTTCAGGA CGAAATATGA CTGGGATTTA 
CTTGCTGCAC GTTCCATCTG GGCTTTTGGC CCTGACAAAC AGGGGCCAAA CATATTATTG 
GATGACACCC TTCCAACTGA GGTGGACAGG AACTTGATGA TGGCAGTGAA AGACTCCATT 
GTTCAAGGGT TCCAGTGGGG TGCTCGTGAA GGTCCCCTCT GTGATGAGCC CATCAGAAAT 
GTAAAGTTCA AGATTGTTGA TGCACGGATA GCACCAGAGC CATTGCATAG AGGATCTGGT 
CAGATGATTC CAACAGCAAG ACGTGTTGCA TATTCAGCTT TTCTAATGGC AACTCCAAGG 
CTAATGGAAC CTGTCTACTA TGTGGAGATA CAAACACCAA TCGACTGTGT AACTGCAATC 
TACACAGTTT TGTCACGTAG ACGTGGGCAT GTCACATCTG ATGTTCCTCA ACCCGGTACT 
CCAGCTTATA TCGTGAAGGC CTTTCTGCCT GTGATAGAGT CCTTTGGGTT TGAGACAGAT 
CTGAGGTACC ACACTCAGGG ACAAGCCTTT TGCCTATCTG TGTTTGATCA CTGGGCCATT 
GTGCCAGGAG ACCCTCTAGA CAAAGCCATC CAGTTGCGCC CACTTGAGCC CGCTCCTATT 
CAGCATCTAG CTCGGGAATT CATGGTCAAG ACCCGCCGTA GAAAGGGAAT GAGCGAAGAC 
GTCAGCGGAA ATAAATTCTT TGATGAAGCG ATGATGGTAG AGCTTGCCCA GCAAACAGGC 
GATCTCCATC TGCAGATGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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MEE5 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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