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ACO2 cDNA ORF clone, Bos taurus(cattle)

Summary

Gene Symbol ACO2
Entrez Gene ID 280976
Full Name aconitase 2
General protein information
Preferred Names

aconitase 2

Names

aconitate hydratase, mitochondrial
aconitase 2, mitochondrial
citrate hydro-lyase
Gene Type protein-coding
Organism Bos taurus(cattle)
Genome

5

5q35

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_173977.3 NP_776402.1 aconitate hydratase, mitochondrial precursor

Pathways from BioSystems


Homologies


Mus musculus (house mouse) Aco2 NP_542364.1
Caenorhabditis elegans (roundworm) aco-2 NP_741235.1
Saccharomyces cerevisiae (baker's yeast) ACO1 NP_013407.1
Canis lupus familiaris (dog) ACO2 XP_849166.1
Gallus gallus (chicken) ACO2 NP_989519.1
ACO2 XP_001105023.1
Drosophila melanogaster (fruit fly) Acon NP_001163030.1
Bos taurus (cattle) ACO2 NP_776402.1
Rattus norvegicus (Norway rat) Aco2 NP_077374.2
Danio rerio (zebrafish) aco2 NP_944590.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP007852 XP_317642.4
Eremothecium gossypii AGOS_ADL032W NP_984065.1
Schizosaccharomyces pombe (fission yeast) SPAC24C9.06c NP_594031.2
Magnaporthe oryzae (rice blast fungus) MGG_03521 XP_003716398.1
Neurospora crassa NCU02366 XP_959787.1
Xenopus tropicalis (tropical clawed frog) aco2 NP_001011445.1
Homo sapiens (human) ACO2 NP_001089.1
Kluyveromyces lactis KLLA0C17314g XP_452974.1

Gene Ontology


Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Isolation of coding sequences from bovine cosmids by means of exon trapping.
Comincini S, Drisaldi B, Ferretti L
Mammalian genome : official journal of the International Mammalian Genome Society8(7)486-90(1997 Jul)

Crystal structures of aconitase with isocitrate and nitroisocitrate bound.
Lauble H, Kennedy MC, Beinert H, Stout CD
Biochemistry31(10)2735-48(1992 Mar)

Cysteine labeling studies of beef heart aconitase containing a 4Fe, a cubane 3Fe, or a linear 3Fe cluster.
Plank DW, Kennedy MC, Beinert H, Howard JB
The Journal of biological chemistry264(34)20385-93(1989 Dec)

Identification of the reactive sulfhydryl and sequences of cysteinyl-tryptic peptides from beef heart aconitase.
Plank DW, Howard JB
The Journal of biological chemistry263(17)8184-9(1988 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ACO2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACO2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa01288 NM_173977.3
Latest version!		 Bos taurus aconitase 2 (ACO2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OBa01288
Clone ID Related Accession (Same CDS sequence) NM_173977.3
Accession Version NM_173977.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2343bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-25
Organism Bos taurus(cattle)
Product aconitate hydratase, mitochondrial precursor
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from Z49931.1. On Aug 31, 2012 this sequence version replaced NM_173977.2. ##Evidence-Data-START## Transcript exon combination :: Z49931.1, BC102642.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGCGCCTT ACAGCCTACT GGTGTCCCGG CTGCAGAAAG CCCTGGGTGC GCGGCAGTAC 
CATGTGGCCT CAGTCCTGTG CCAACGGGCC AAGGTGGCCA TGAGCCACTT TGAACCCAAT 
GAGTACATCC GCTATGACCT GCTCGAGAAG AACATTAACA TTGTCCGCAA ACGGCTGAAC 
CGGCCTCTGA CCCTCTCAGA GAAGATCGTG TATGGACACC TGGATGACCC GGCCAACCAG 
GAGATCGAGC GGGGCAAGAC ATACCTGCGG CTGCGGCCTG ACCGCGTGGC CATGCGGGAT 
GCCACGGCCC AGATGGCCAT GCTGCAGTTC ATCAGCAGCG GCCTGCCCAA GGTGGCCGTG 
CCGTCCACCA TCCACTGTGA CCATTTGATC GAGGCCCAGC TCGGCGGGGA GAAGGACCTG 
CGTCGGGCCA AGGACATAAA CCAGGAAGTG TATAATTTCC TGGCCACTGC AGGTGCCAAG 
TACGGCGTGG GCTTCTGGAG GCCTGGCTCT GGAATCATTC ACCAGATCAT TCTGGAAAAC 
TATGCGTACC CTGGGGTTCT TCTGATTGGC ACTGATTCCC ACACCCCTAA TGGCGGTGGC 
CTGGGAGGCA TCTGCATTGG AGTCGGGGGT GCTGATGCCG TGGACGTCAC GGCTGGGATC 
CCCTGGGAGT TGAAGTGCCC CAAGGTGATT GGCGTGAAGC TGACAGGCTC CCTCTCTGGC 
TGGACCTCAC CTAAAGATGT GATCCTGAAG GTGGCGGGTA TCCTCACAGT GAAAGGTGGC 
ACGGGCGCCA TCGTGGAGTA CCACGGGCCT GGAGTAGACT CCATCTCCTG CACCGGCATG 
GCGACCATCT GCAACATGGG TGCAGAAATC GGGGCCACCA CTTCGGTGTT CCCTTACAAC 
CACAGGATGA AGAAATACCT GAGCAAGACA GGCCGGGCAG ATATTGCCAA CCTTGCTGAT 
GAATTCAAGG ATCACTTGGT ACCTGACTCT GGCTGCCATT ATGACCAACT GATTGAAATT 
AACCTCAGTG AGCTGAAGCC GCACATCAAT GGGCCCTTTA CCCCTGACCT GGCTCACCCT 
GTGGCAGAAG TGGGCAGTGT GGCAGAGAAG GAGGGGTGGC CCTTGGACAT CCGAGTTGGT 
CTGATTGGCA GCTGTACCAA CTCCAGCTAT GAAGACATGG GCCGCTCCGC AGCCGTGGCC 
AAGCAGGCGC TGGCCCACGG ACTCCAGTGC AAATCCCAGT TCACCATCAC CCCAGGCTCG 
GAGCAGATCC GCGCCACCAT CGAGCGGGAT GGCTATGCAC AGATCCTGAG GGATGTGGGT 
GGCATCGTCC TGGCCAATGC CTGCGGGCCC TGCATTGGCC AATGGGACAG GAAGGACATC 
AAGAAGGGGG AGAAGAACAC CATCGTCACC TCCTACAACA GGAACTTCAC AGGCCGCAAC 
GACGCAAATC CTGAGACCCA CGCCTTCGTC ACGTCCCCAG AGATTGTCAC AGCCCTGGCC 
ATTGCTGGCA CCCTCAAGTT CAACCCAGAG ACTGACTTCC TGACGGGCAA GGACGGCAAG 
AAGTTCAAGC TGGAGGCTCC AGACGCAGAT GAGCTTCCTC GAGCGGAGTT CGACCCCGGA 
CAGGACACCT ACCAGCACCC CCCCAAGGAC AGCAGTGGGC AGCAGGTGGA CGTGAGTCCC 
ACCAGCCAGC GCCTGCAGCT CCTGGAGCCT TTCGACAAGT GGGACGGCCG AGACCTGGAG 
GACCTGCAGA TCCTCATCAA GGTCAAAGGG AAGTGTACCA CCGACCACAT CTCGGCTGCT 
GGTCCCTGGC TCAAGTTCCG TGGGCACCTG GACAACATCT CCAACAACCT GCTCATTGGC 
GCCATCAACG TGGAAAACGG CAAGGCCAAC TCCGTGCGCA ATGCCGTCAC CCAGGAGTTC 
GGTCCTGTCC CTGACACCGC CCGCTACTAC AAGAAACACG GCATCCGGTG GGTGGTGATT 
GGAGATGAAA ACTATGGTGA GGGCTCGAGC CGGGAGCACG CAGCCCTGGA GCCTCGCCAC 
CTCGGGGGCC GGGCCATCAT CACCAAGAGC TTTGCCAGGA TCCACGAAAC CAACCTGAAG 
AAGCAGGGCC TGCTGCCCCT CACCTTCGCT GACCCAGCTG ACTACAACAA GATTCACCCT 
GTGGACAAGC TGACCATCAA GGGCCTGAAG GACTTTGCCC CCGGCAAGCC GCTGACATGT 
ATCATCAAGC ACCCCAACGG GACCCAGGAG ACCATCCTCC TGAACCACAC CTTCAACGAG 
ACCCAGATCG AGTGGTTCCG CGCCGGCAGC GCGCTCAACA GGATGAAGGA GCTGCAGAAG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_776402.1
CDS30..2372
Translation

Target ORF information:

RefSeq Version NM_173977.3
Organism Bos taurus(cattle)
Definition Bos taurus aconitase 2 (ACO2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_173977.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
ATGGCGCCTT ACAGCCTACT GGTGTCCCGG CTGCAGAAAG CCCTGGGTGC GCGGCAGTAC 
CATGTGGCCT CAGTCCTGTG CCAACGGGCC AAGGTGGCCA TGAGCCACTT TGAACCCAAT 
GAGTACATCC GCTATGACCT GCTCGAGAAG AACATTAACA TTGTCCGCAA ACGGCTGAAC 
CGGCCTCTGA CCCTCTCAGA GAAGATCGTG TATGGACACC TGGATGACCC GGCCAACCAG 
GAGATCGAGC GGGGCAAGAC ATACCTGCGG CTGCGGCCTG ACCGCGTGGC CATGCGGGAT 
GCCACGGCCC AGATGGCCAT GCTGCAGTTC ATCAGCAGCG GCCTGCCCAA GGTGGCCGTG 
CCGTCCACCA TCCACTGTGA CCATTTGATC GAGGCCCAGC TCGGCGGGGA GAAGGACCTG 
CGTCGGGCCA AGGACATAAA CCAGGAAGTG TATAATTTCC TGGCCACTGC AGGTGCCAAG 
TACGGCGTGG GCTTCTGGAG GCCTGGCTCT GGAATCATTC ACCAGATCAT TCTGGAAAAC 
TATGCGTACC CTGGGGTTCT TCTGATTGGC ACTGATTCCC ACACCCCTAA TGGCGGTGGC 
CTGGGAGGCA TCTGCATTGG AGTCGGGGGT GCTGATGCCG TGGACGTCAC GGCTGGGATC 
CCCTGGGAGT TGAAGTGCCC CAAGGTGATT GGCGTGAAGC TGACAGGCTC CCTCTCTGGC 
TGGACCTCAC CTAAAGATGT GATCCTGAAG GTGGCGGGTA TCCTCACAGT GAAAGGTGGC 
ACGGGCGCCA TCGTGGAGTA CCACGGGCCT GGAGTAGACT CCATCTCCTG CACCGGCATG 
GCGACCATCT GCAACATGGG TGCAGAAATC GGGGCCACCA CTTCGGTGTT CCCTTACAAC 
CACAGGATGA AGAAATACCT GAGCAAGACA GGCCGGGCAG ATATTGCCAA CCTTGCTGAT 
GAATTCAAGG ATCACTTGGT ACCTGACTCT GGCTGCCATT ATGACCAACT GATTGAAATT 
AACCTCAGTG AGCTGAAGCC GCACATCAAT GGGCCCTTTA CCCCTGACCT GGCTCACCCT 
GTGGCAGAAG TGGGCAGTGT GGCAGAGAAG GAGGGGTGGC CCTTGGACAT CCGAGTTGGT 
CTGATTGGCA GCTGTACCAA CTCCAGCTAT GAAGACATGG GCCGCTCCGC AGCCGTGGCC 
AAGCAGGCGC TGGCCCACGG ACTCCAGTGC AAATCCCAGT TCACCATCAC CCCAGGCTCG 
GAGCAGATCC GCGCCACCAT CGAGCGGGAT GGCTATGCAC AGATCCTGAG GGATGTGGGT 
GGCATCGTCC TGGCCAATGC CTGCGGGCCC TGCATTGGCC AATGGGACAG GAAGGACATC 
AAGAAGGGGG AGAAGAACAC CATCGTCACC TCCTACAACA GGAACTTCAC AGGCCGCAAC 
GACGCAAATC CTGAGACCCA CGCCTTCGTC ACGTCCCCAG AGATTGTCAC AGCCCTGGCC 
ATTGCTGGCA CCCTCAAGTT CAACCCAGAG ACTGACTTCC TGACGGGCAA GGACGGCAAG 
AAGTTCAAGC TGGAGGCTCC AGACGCAGAT GAGCTTCCTC GAGCGGAGTT CGACCCCGGA 
CAGGACACCT ACCAGCACCC CCCCAAGGAC AGCAGTGGGC AGCAGGTGGA CGTGAGTCCC 
ACCAGCCAGC GCCTGCAGCT CCTGGAGCCT TTCGACAAGT GGGACGGCCG AGACCTGGAG 
GACCTGCAGA TCCTCATCAA GGTCAAAGGG AAGTGTACCA CCGACCACAT CTCGGCTGCT 
GGTCCCTGGC TCAAGTTCCG TGGGCACCTG GACAACATCT CCAACAACCT GCTCATTGGC 
GCCATCAACG TGGAAAACGG CAAGGCCAAC TCCGTGCGCA ATGCCGTCAC CCAGGAGTTC 
GGTCCTGTCC CTGACACCGC CCGCTACTAC AAGAAACACG GCATCCGGTG GGTGGTGATT 
GGAGATGAAA ACTATGGTGA GGGCTCGAGC CGGGAGCACG CAGCCCTGGA GCCTCGCCAC 
CTCGGGGGCC GGGCCATCAT CACCAAGAGC TTTGCCAGGA TCCACGAAAC CAACCTGAAG 
AAGCAGGGCC TGCTGCCCCT CACCTTCGCT GACCCAGCTG ACTACAACAA GATTCACCCT 
GTGGACAAGC TGACCATCAA GGGCCTGAAG GACTTTGCCC CCGGCAAGCC GCTGACATGT 
ATCATCAAGC ACCCCAACGG GACCCAGGAG ACCATCCTCC TGAACCACAC CTTCAACGAG 
ACCCAGATCG AGTGGTTCCG CGCCGGCAGC GCGCTCAACA GGATGAAGGA GCTGCAGAAG 
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL


book

Isolation of coding sequences from bovine cosmids by means of exon trapping.
Mammalian genome : official journal of the International Mammalian Genome Society8(7)486-90(1997 Jul)
Comincini S,Drisaldi B,Ferretti L


book

Crystal structures of aconitase with isocitrate and nitroisocitrate bound.
Biochemistry31(10)2735-48(1992 Mar)
Lauble H,Kennedy MC,Beinert H,Stout CD


book

Cysteine labeling studies of beef heart aconitase containing a 4Fe, a cubane 3Fe, or a linear 3Fe cluster.
The Journal of biological chemistry264(34)20385-93(1989 Dec)
Plank DW,Kennedy MC,Beinert H,Howard JB


book

Identification of the reactive sulfhydryl and sequences of cysteinyl-tryptic peptides from beef heart aconitase.
The Journal of biological chemistry263(17)8184-9(1988 Jun)
Plank DW,Howard JB