COL1A1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL1A1
Entrez Gene ID	282187
Full Name	collagen type I alpha 1 chain
General protein information	Preferred Names collagen type I alpha 1 chain Names collagen alpha-1(I) chain alpha-1 type I collagen collagen, type I, alpha 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024979627.1	XP_024835395.1	collagen alpha-1(I) chain isoform X1
NM_001034039.2	NP_001029211.1	collagen alpha-1(I) chain precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	COL1A1	NP_001029211.1
Mus musculus (house mouse)	Col1a1	NP_031768.2
Danio rerio (zebrafish)	col1a1a	NP_954684.1
Pan troglodytes (chimpanzee)	COL1A1	XP_001169409.1
	COL1A1	XP_001096194.2
Canis lupus familiaris (dog)	COL1A1	NP_001003090.1
Rattus norvegicus (Norway rat)	Col1a1	NP_445756.1
Homo sapiens (human)	COL1A1	NP_000079.2
Xenopus tropicalis (tropical clawed frog)	LOC496414	NP_001011005.1

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of Microfibrillar-associated Protein 4 (MFAP4) as a Tropoelastin- and Fibrillin-binding Protein Involved in Elastic Fiber Formation.
Pilecki B, Holm AT, Schlosser A, Moeller JB, Wohl AP, Zuk AV, Heum?ller SE, Wallis R, Moestrup SK, Sengle G, Holmskov U, Sorensen GL
The Journal of biological chemistry291(3)1103-14(2016 Jan)

Developmental Stage-dependent Regulation of Prolyl 3-Hydroxylation in Tendon Type I Collagen.
Taga Y, Kusubata M, Ogawa-Goto K, Hattori S
The Journal of biological chemistry291(2)837-47(2016 Jan)

Snail1 induced in breast cancer cells in 3D collagen I gel environment suppresses cortactin and impairs effective invadopodia formation.
Lee MS, Kim S, Kim BG, Won C, Nam SH, Kang S, Kim HJ, Kang M, Ryu J, Song HE, Lee D, Ye SK, Jeon NL, Kim TY, Cho NH, Lee JW
Biochimica et biophysica acta1843(9)2037-54(2014 Sep)

The aggregation behavior of native collagen in dilute solution studied by intrinsic fluorescence and external probing.
Wu K, Liu W, Li G
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy102186-93(2013 Feb)

Two subsystems of meniscal collagen and their different thermal stabilities.
Ignatieva NY, Zakharkina OL, Lunin VV, Baratova LA, Grokhovskaya TE, Balalaeva IV, Sergeeva EA
Doklady. Biochemistry and biophysics444175-7(2012 May-Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The present findings suggest that prolyl 3-hydroxylation incrementally regulates collagen fibril diameter in tendon.
Title: Developmental Stage-dependent Regulation of Prolyl 3-Hydroxylation in Tendon Type I Collagen.

These observations support a signaling network among JNKs, Smads, Snail1, and cortactin to regulate the invasion of MDA-MB-231 cells embedded in 3D collagen I, which may be targeted during screening of anti-invasion reagents.
Title: Snail1 induced in breast cancer cells in 3D collagen I gel environment suppresses cortactin and impairs effective invadopodia formation.

The experiment results demonstrated that the aggregates of collagen incurred in the range of 0.30-0.45 mg/mL, while the more complicated ones emerged at higher concentrations of collagen (0.75 and 1.05 mg/mL).
Title: The aggregation behavior of native collagen in dilute solution studied by intrinsic fluorescence and external probing.

Two subsystems of meniscal collagen and their different thermal stabilities.
Title: Two subsystems of meniscal collagen and their different thermal stabilities.

These results imply that the fibril form of collagen plays a potential role in inhibiting the growth of cancer cells.
Title: Delay of cell cycle progression and induction death of cancer cells on type I collagen fibrils [corrected].

The following COL1A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL1A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa225981	XM_024979627.1 Latest version!	Bos taurus collagen type I alpha 1 chain (COL1A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa00449	NM_001034039.2 Latest version!	Bos taurus collagen type I alpha 1 chain (COL1A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL1A1 ( XM_024979627.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835395.1 Bos taurus collagen type I alpha 1 chain (COL1A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa225981
Clone ID Related Accession (Same CDS sequence)	XM_024979627.1
Accession Version	XM_024979627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	collagen alpha-1(I) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCT TTGTGGACCT CCGGCTCCTG CTCCTCTTAG CGGCCACCGC CCTCCTGACG 
CACGGCCAAG AGGAGGGCCA GGAAGAAGGC CAAGAAGAAG ACATCCCACC AGTCACCTGC 
GTACAGAACG GCCTCAGGTA CCATGACCGA GACGTGTGGA AACCCGTGCC CTGCCAGATC 
TGTGTCTGCG ACAACGGCAA CGTGCTGTGC GATGACGTGA TCTGCGACGA ACTTAAGGAC 
TGTCCTAACG CCAAAGTCCC CACGGACGAA TGCTGCCCCG TCTGCCCCGA AGGCCAGGAA 
TCACCCACGG ACCAAGAAAC CACCGGAGTC GAGGGACCGA AAGGAGACAC TGGCCCCCGA 
GGCCCAAGGG GACCCGCCGG CCCCCCCGGC CGAGATGGCA TCCCTGGACA ACCTGGACTT 
CCCGGACCCC CTGGACCCCC CGGACCTCCC GGACCCCCTG GCCTCGGAGG AAACTTTGCT 
CCCCAGTTGT CTTACGGCTA TGATGAGAAA TCAACAGGAA TTTCCGTGCC TGGTCCCATG 
GGTCCTTCTG GTCCTCGTGG TCTCCCTGGC CCCCCTGGCG CACCTGGTCC CCAAGGTTTC 
CAAGGCCCCC CTGGTGAGCC TGGCGAGCCA GGAGCCTCAG GTCCCATGGG TCCCCGTGGT 
CCCCCTGGCC CCCCTGGCAA GAACGGAGAT GATGGCGAAG CTGGAAAGCC TGGTCGTCCT 
GGTGAGCGCG GGCCTCCCGG ACCTCAGGGT GCTCGGGGAT TGCCTGGAAC AGCTGGCCTC 
CCTGGAATGA AGGGACACAG AGGTTTCAGT GGTTTGGATG GTGCCAAGGG AGATGCTGGT 
CCTGCTGGCC CCAAGGGCGA GCCTGGTAGC CCCGGTGAAA ATGGAGCTCC TGGTCAGATG 
GGCCCCCGTG GTCTGCCTGG TGAGAGAGGT CGCCCTGGAG CCCCTGGCCC TGCTGGTGCT 
CGAGGAAATG ATGGTGCGAC TGGTGCTGCT GGGCCCCCTG GTCCCACTGG CCCCGCTGGT 
CCTCCTGGTT TCCCTGGTGC TGTGGGTGCT AAGGGTGAAG GTGGTCCCCA AGGACCCCGA 
GGTTCTGAAG GTCCCCAGGG TGTACGTGGT GAGCCTGGCC CCCCTGGCCC TGCTGGTGCT 
GCTGGCCCTG CTGGCAACCC TGGTGCTGAT GGACAGCCTG GTGCTAAAGG AGCCAATGTA 
AGTATCCTGC AGGTCTCAGA GCCGCCCCCG GTACCCGCCT TCCACCTGCC TTTGCCCTCC 
AGTCCTGGGC TCAATTTGTT TGGCCTCCCT CAGGGCGCTC CTGGTATTGC TGGTGCTCCT 
GGCTTCCCTG GTGCCCGAGG CCCCTCTGGA CCCCAGGGCC CCAGCGGCCC CCCTGGCCCC 
AAGGGTAACA GCGGTGAACC TGGTGCTCCT GGCAGCAAAG GAGACACTGG CGCCAAGGGA 
GAACCCGGTC CCACTGGTAT TCAAGGCCCC CCTGGCCCCG CTGGGGAAGA AGGAAAGCGA 
GGAGCCCGAG GTGAACCTGG ACCTGCTGGC CTGCCTGGAC CCCCTGGCGA GCGTGGTGGA 
CCTGGAAGCC GTGGTTTCCC TGGCGCCGAC GGTGTTGCTG GTCCCAAGGG TCCTGCTGGT 
GAACGCGGTG CTCCTGGCCC TGCTGGCCCC AAAGGTTCTC CTGGTGAAGC TGGTCGCCCC 
GGTGAAGCTG GTCTGCCCGG TGCCAAGGGT CTGACTGGAA GCCCTGGCAG CCCGGGTCCT 
GATGGCAAAA CTGGCCCCCC TGGTCCCGCC GGTCAAGATG GCCGCCCTGG ACCTCCAGGC 
CCTCCCGGTG CCCGTGGTCA GGCTGGCGTG ATGGGTTTCC CTGGACCTAA AGGTGCTGCT 
GGAGAGCCTG GAAAAGCTGG AGAGCGAGGT GTTCCTGGAC CCCCTGGCGC TGTTGGTCCT 
GCTGGCAAAG ACGGAGAAGC TGGAGCTCAG GGACCCCCAG GACCTGCTGG CCCCGCTGGT 
GAGAGAGGCG AACAAGGCCC TGCTGGCTCC CCTGGATTCC AGGGTCTCCC CGGCCCTGCT 
GGTCCTCCTG GTGAAGCAGG CAAACCTGGT GAACAGGGTG TTCCTGGAGA TCTTGGTGCC 
CCCGGCCCCT CTGGAGCAAG AGGCGAGAGA GGTTTCCCCG GCGAGCGTGG TGTGCAAGGG 
CCGCCCGGTC CTGCAGGTCC CCGTGGGGCC AATGGTGCCC CTGGCAACGA TGGTGCTAAG 
GGTGATGCTG GTGCCCCTGG AGCCCCCGGT AGCCAGGGTG CCCCTGGCCT TCAAGGAATG 
CCTGGTGAAC GAGGTGCAGC TGGTCTTCCA GGCCCTAAGG GTGACAGAGG GGATGCTGGT 
CCCAAAGGTG CTGATGGTGC TCCTGGCAAA GATGGCGTCC GTGGTCTGAC TGGTCCCATC 
GGTCCTCCTG GCCCCGCTGG TGCCCCTGGT GACAAGGGTG AAGCTGGTCC TAGTGGCCCA 
GCCGGTCCCA CTGGAGCTCG TGGTGCCCCC GGTGACCGTG GTGAGCCTGG TCCCCCCGGC 
CCTGCTGGCT TCGCTGGCCC CCCTGGTGCT GATGGCCAAC CTGGTGCTAA AGGCGAACCT 
GGTGATGCTG GTGCTAAAGG TGACGCTGGT CCCCCCGGCC CTGCTGGGCC CGCTGGACCC 
CCCGGCCCCA TTGGTAACGT TGGTGCTCCC GGACCCAAAG GTGCTCGTGG CAGCGCTGGT 
CCCCCTGGTG CTACTGGTTT CCCAGGTGCT GCTGGCCGAG TCGGTCCCCC CGGCCCCTCT 
GGAAATGCTG GACCCCCTGG CCCTCCTGGC CCTGCTGGCA AAGAAGGCAG CAAAGGCCCC 
CGCGGTGAGA CTGGCCCCGC TGGGCGTCCC GGTGAAGTCG GTCCCCCTGG TCCCCCTGGC 
CCCGCTGGTG AGAAAGGAGC CCCTGGTGCT GACGGACCTG CTGGAGCTCC TGGCACTCCT 
GGACCTCAAG GTATTGCTGG ACAGCGTGGT GTGGTCGGCC TGCCTGGTCA GAGAGGAGAA 
AGAGGCTTCC CTGGTCTTCC TGGCCCCTCT GGTGAACCCG GCAAACAAGG TCCTTCTGGA 
GCAAGTGGTG AACGTGGCCC CCCTGGTCCC ATGGGCCCCC CTGGATTGGC TGGACCCCCT 
GGCGAGTCTG GACGTGAGGG AGCTCCTGGT GCTGAAGGAT CCCCTGGACG AGATGGTTCT 
CCTGGCGCCA AGGGTGACCG TGGTGAGACC GGCCCTGCTG GACCTCCTGG TGCTCCTGGC 
GCTCCCGGTG CCCCCGGCCC TGTCGGACCT GCCGGCAAGA GCGGTGATCG TGGTGAGACC 
GGTCCTGCTG GTCCTGCTGG TCCCATTGGC CCCGTTGGTG CCCGTGGCCC CGCTGGACCC 
CAAGGCCCCC GTGGTGACAA GGGTGAGACA GGCGAACAGG GCGACAGAGG CATTAAGGGT 
CACCGTGGCT TCTCTGGTCT CCAGGGTCCC CCCGGCCCTC CCGGCTCTCC TGGTGAGCAA 
GGTCCTTCCG GAGCCTCTGG TCCTGCTGGT CCCCGCGGTC CCCCTGGCTC TGCTGGTTCT 
CCCGGCAAAG ATGGACTCAA TGGTCTCCCA GGCCCCATCG GTCCCCCTGG GCCTCGAGGT 
CGCACTGGTG ATGCTGGTCC TGCTGGTCCT CCCGGCCCTC CTGGACCCCC TGGTCCCCCA 
GGTCCTCCCA GCGGCGGCTA CGACTTGAGC TTCCTGCCCC AGCCACCTCA AGAGAAGGCT 
CACGATGGTG GCCGCTACTA CCGGGCTGAT GATGCCAATG TGGTCCGTGA CCGTGACCTC 
GAGGTGGACA CCACCCTCAA GAGCCTGAGC CAGCAGATCG AGAACATCCG GAGCCCTGAA 
GGCAGCCGCA AGAACCCCGC CCGCACCTGC CGTGACCTCA AGATGTGCCA CTCTGACTGG 
AAGAGCGGAG AATACTGGAT TGACCCCAAC CAAGGCTGCA ACCTGGATGC CATTAAGGTC 
TTCTGCAACA TGGAAACCGG TGAGACCTGT GTATACCCCA CTCAGCCCAG CGTGGCCCAG 
AAGAACTGGT ATATCAGCAA GAACCCCAAG GAAAAGAGGC ACGTCTGGTA CGGCGAGAGC 
ATGACCGGCG GATTCCAGTT CGAGTATGGC GGCCAGGGGT CCGATCCTGC CGATGTGGCC 
ATCCAGCTGA CTTTCCTGCG CCTGATGTCC ACCGAGGCCT CCCAGAACAT CACCTACCAC 
TGCAAGAACA GCGTGGCCTA CATGGACCAG CAGACTGGCA ACCTCAAGAA GGCCCTGCTC 
CTCCAGGGCT CCAACGAGAT CGAGATCCGG GCCGAGGGCA ACAGCCGCTT CACCTACAGC 
GTCACCTACG ATGGCTGCAC GAGTCACACC GGAGCCTGGG GCAAGACAGT GATCGAATAC 
AAAACCACCA AGACCTCCCG CTTGCCCATC ATCGATGTGG CCCCCTTGGA CGTTGGCGCC 
CCAGACCAGG AATTCGGCTT CGACGTTGGC CCTGCCTGCT TCCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835395.1
CDS	129..4616
Translation	MFSFVDLRLLLLLAATALLTHGQEEGQEEGQEEDIPPVTCVQNGLRYHDRDVWKPVPCQICVCDNGNVLCDDVICDELKDCPNAKVPTDECCPVCPEGQESPTDQETTGVEGPKGDTGPRGPRGPAGPPGRDGIPGQPGLPGPPGPPGPPGPPGLGGNFAPQLSYGYDEKSTGISVPGPMGPSGPRGLPGPPGAPGPQGFQGPPGEPGEPGASGPMGPRGPPGPPGKNGDDGEAGKPGRPGERGPPGPQGARGLPGTAGLPGMKGHRGFSGLDGAKGDAGPAGPKGEPGSPGENGAPGQMGPRGLPGERGRPGAPGPAGARGNDGATGAAGPPGPTGPAGPPGFPGAVGAKGEGGPQGPRGSEGPQGVRGEPGPPGPAGAAGPAGNPGADGQPGAKGANVSILQVSEPPPVPAFHLPLPSSPGLNLFGLPQGAPGIAGAPGFPGARGPSGPQGPSGPPGPKGNSGEPGAPGSKGDTGAKGEPGPTGIQGPPGPAGEEGKRGARGEPGPAGLPGPPGERGGPGSRGFPGADGVAGPKGPAGERGAPGPAGPKGSPGEAGRPGEAGLPGAKGLTGSPGSPGPDGKTGPPGPAGQDGRPGPPGPPGARGQAGVMGFPGPKGAAGEPGKAGERGVPGPPGAVGPAGKDGEAGAQGPPGPAGPAGERGEQGPAGSPGFQGLPGPAGPPGEAGKPGEQGVPGDLGAPGPSGARGERGFPGERGVQGPPGPAGPRGANGAPGNDGAKGDAGAPGAPGSQGAPGLQGMPGERGAAGLPGPKGDRGDAGPKGADGAPGKDGVRGLTGPIGPPGPAGAPGDKGEAGPSGPAGPTGARGAPGDRGEPGPPGPAGFAGPPGADGQPGAKGEPGDAGAKGDAGPPGPAGPAGPPGPIGNVGAPGPKGARGSAGPPGATGFPGAAGRVGPPGPSGNAGPPGPPGPAGKEGSKGPRGETGPAGRPGEVGPPGPPGPAGEKGAPGADGPAGAPGTPGPQGIAGQRGVVGLPGQRGERGFPGLPGPSGEPGKQGPSGASGERGPPGPMGPPGLAGPPGESGREGAPGAEGSPGRDGSPGAKGDRGETGPAGPPGAPGAPGAPGPVGPAGKSGDRGETGPAGPAGPIGPVGARGPAGPQGPRGDKGETGEQGDRGIKGHRGFSGLQGPPGPPGSPGEQGPSGASGPAGPRGPPGSAGSPGKDGLNGLPGPIGPPGPRGRTGDAGPAGPPGPPGPPGPPGPPSGGYDLSFLPQPPQEKAHDGGRYYRADDANVVRDRDLEVDTTLKSLSQQIENIRSPEGSRKNPARTCRDLKMCHSDWKSGEYWIDPNQGCNLDAIKVFCNMETGETCVYPTQPSVAQKNWYISKNPKEKRHVWYGESMTGGFQFEYGGQGSDPADVAIQLTFLRLMSTEASQNITYHCKNSVAYMDQQTGNLKKALLLQGSNEIEIRAEGNSRFTYSVTYDGCTSHTGAWGKTVIEYKTTKTSRLPIIDVAPLDVGAPDQEFGFDVGPACFL

Target ORF information:

RefSeq Version	XM_024979627.1
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type I alpha 1 chain (COL1A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979627.1

ORF Insert Sequence:

ATGTTCAGCT TTGTGGACCT CCGGCTCCTG CTCCTCTTAG CGGCCACCGC CCTCCTGACG 
CACGGCCAAG AGGAGGGCCA GGAAGAAGGC CAAGAAGAAG ACATCCCACC AGTCACCTGC 
GTACAGAACG GCCTCAGGTA CCATGACCGA GACGTGTGGA AACCCGTGCC CTGCCAGATC 
TGTGTCTGCG ACAACGGCAA CGTGCTGTGC GATGACGTGA TCTGCGACGA ACTTAAGGAC 
TGTCCTAACG CCAAAGTCCC CACGGACGAA TGCTGCCCCG TCTGCCCCGA AGGCCAGGAA 
TCACCCACGG ACCAAGAAAC CACCGGAGTC GAGGGACCGA AAGGAGACAC TGGCCCCCGA 
GGCCCAAGGG GACCCGCCGG CCCCCCCGGC CGAGATGGCA TCCCTGGACA ACCTGGACTT 
CCCGGACCCC CTGGACCCCC CGGACCTCCC GGACCCCCTG GCCTCGGAGG AAACTTTGCT 
CCCCAGTTGT CTTACGGCTA TGATGAGAAA TCAACAGGAA TTTCCGTGCC TGGTCCCATG 
GGTCCTTCTG GTCCTCGTGG TCTCCCTGGC CCCCCTGGCG CACCTGGTCC CCAAGGTTTC 
CAAGGCCCCC CTGGTGAGCC TGGCGAGCCA GGAGCCTCAG GTCCCATGGG TCCCCGTGGT 
CCCCCTGGCC CCCCTGGCAA GAACGGAGAT GATGGCGAAG CTGGAAAGCC TGGTCGTCCT 
GGTGAGCGCG GGCCTCCCGG ACCTCAGGGT GCTCGGGGAT TGCCTGGAAC AGCTGGCCTC 
CCTGGAATGA AGGGACACAG AGGTTTCAGT GGTTTGGATG GTGCCAAGGG AGATGCTGGT 
CCTGCTGGCC CCAAGGGCGA GCCTGGTAGC CCCGGTGAAA ATGGAGCTCC TGGTCAGATG 
GGCCCCCGTG GTCTGCCTGG TGAGAGAGGT CGCCCTGGAG CCCCTGGCCC TGCTGGTGCT 
CGAGGAAATG ATGGTGCGAC TGGTGCTGCT GGGCCCCCTG GTCCCACTGG CCCCGCTGGT 
CCTCCTGGTT TCCCTGGTGC TGTGGGTGCT AAGGGTGAAG GTGGTCCCCA AGGACCCCGA 
GGTTCTGAAG GTCCCCAGGG TGTACGTGGT GAGCCTGGCC CCCCTGGCCC TGCTGGTGCT 
GCTGGCCCTG CTGGCAACCC TGGTGCTGAT GGACAGCCTG GTGCTAAAGG AGCCAATGTA 
AGTATCCTGC AGGTCTCAGA GCCGCCCCCG GTACCCGCCT TCCACCTGCC TTTGCCCTCC 
AGTCCTGGGC TCAATTTGTT TGGCCTCCCT CAGGGCGCTC CTGGTATTGC TGGTGCTCCT 
GGCTTCCCTG GTGCCCGAGG CCCCTCTGGA CCCCAGGGCC CCAGCGGCCC CCCTGGCCCC 
AAGGGTAACA GCGGTGAACC TGGTGCTCCT GGCAGCAAAG GAGACACTGG CGCCAAGGGA 
GAACCCGGTC CCACTGGTAT TCAAGGCCCC CCTGGCCCCG CTGGGGAAGA AGGAAAGCGA 
GGAGCCCGAG GTGAACCTGG ACCTGCTGGC CTGCCTGGAC CCCCTGGCGA GCGTGGTGGA 
CCTGGAAGCC GTGGTTTCCC TGGCGCCGAC GGTGTTGCTG GTCCCAAGGG TCCTGCTGGT 
GAACGCGGTG CTCCTGGCCC TGCTGGCCCC AAAGGTTCTC CTGGTGAAGC TGGTCGCCCC 
GGTGAAGCTG GTCTGCCCGG TGCCAAGGGT CTGACTGGAA GCCCTGGCAG CCCGGGTCCT 
GATGGCAAAA CTGGCCCCCC TGGTCCCGCC GGTCAAGATG GCCGCCCTGG ACCTCCAGGC 
CCTCCCGGTG CCCGTGGTCA GGCTGGCGTG ATGGGTTTCC CTGGACCTAA AGGTGCTGCT 
GGAGAGCCTG GAAAAGCTGG AGAGCGAGGT GTTCCTGGAC CCCCTGGCGC TGTTGGTCCT 
GCTGGCAAAG ACGGAGAAGC TGGAGCTCAG GGACCCCCAG GACCTGCTGG CCCCGCTGGT 
GAGAGAGGCG AACAAGGCCC TGCTGGCTCC CCTGGATTCC AGGGTCTCCC CGGCCCTGCT 
GGTCCTCCTG GTGAAGCAGG CAAACCTGGT GAACAGGGTG TTCCTGGAGA TCTTGGTGCC 
CCCGGCCCCT CTGGAGCAAG AGGCGAGAGA GGTTTCCCCG GCGAGCGTGG TGTGCAAGGG 
CCGCCCGGTC CTGCAGGTCC CCGTGGGGCC AATGGTGCCC CTGGCAACGA TGGTGCTAAG 
GGTGATGCTG GTGCCCCTGG AGCCCCCGGT AGCCAGGGTG CCCCTGGCCT TCAAGGAATG 
CCTGGTGAAC GAGGTGCAGC TGGTCTTCCA GGCCCTAAGG GTGACAGAGG GGATGCTGGT 
CCCAAAGGTG CTGATGGTGC TCCTGGCAAA GATGGCGTCC GTGGTCTGAC TGGTCCCATC 
GGTCCTCCTG GCCCCGCTGG TGCCCCTGGT GACAAGGGTG AAGCTGGTCC TAGTGGCCCA 
GCCGGTCCCA CTGGAGCTCG TGGTGCCCCC GGTGACCGTG GTGAGCCTGG TCCCCCCGGC 
CCTGCTGGCT TCGCTGGCCC CCCTGGTGCT GATGGCCAAC CTGGTGCTAA AGGCGAACCT 
GGTGATGCTG GTGCTAAAGG TGACGCTGGT CCCCCCGGCC CTGCTGGGCC CGCTGGACCC 
CCCGGCCCCA TTGGTAACGT TGGTGCTCCC GGACCCAAAG GTGCTCGTGG CAGCGCTGGT 
CCCCCTGGTG CTACTGGTTT CCCAGGTGCT GCTGGCCGAG TCGGTCCCCC CGGCCCCTCT 
GGAAATGCTG GACCCCCTGG CCCTCCTGGC CCTGCTGGCA AAGAAGGCAG CAAAGGCCCC 
CGCGGTGAGA CTGGCCCCGC TGGGCGTCCC GGTGAAGTCG GTCCCCCTGG TCCCCCTGGC 
CCCGCTGGTG AGAAAGGAGC CCCTGGTGCT GACGGACCTG CTGGAGCTCC TGGCACTCCT 
GGACCTCAAG GTATTGCTGG ACAGCGTGGT GTGGTCGGCC TGCCTGGTCA GAGAGGAGAA 
AGAGGCTTCC CTGGTCTTCC TGGCCCCTCT GGTGAACCCG GCAAACAAGG TCCTTCTGGA 
GCAAGTGGTG AACGTGGCCC CCCTGGTCCC ATGGGCCCCC CTGGATTGGC TGGACCCCCT 
GGCGAGTCTG GACGTGAGGG AGCTCCTGGT GCTGAAGGAT CCCCTGGACG AGATGGTTCT 
CCTGGCGCCA AGGGTGACCG TGGTGAGACC GGCCCTGCTG GACCTCCTGG TGCTCCTGGC 
GCTCCCGGTG CCCCCGGCCC TGTCGGACCT GCCGGCAAGA GCGGTGATCG TGGTGAGACC 
GGTCCTGCTG GTCCTGCTGG TCCCATTGGC CCCGTTGGTG CCCGTGGCCC CGCTGGACCC 
CAAGGCCCCC GTGGTGACAA GGGTGAGACA GGCGAACAGG GCGACAGAGG CATTAAGGGT 
CACCGTGGCT TCTCTGGTCT CCAGGGTCCC CCCGGCCCTC CCGGCTCTCC TGGTGAGCAA 
GGTCCTTCCG GAGCCTCTGG TCCTGCTGGT CCCCGCGGTC CCCCTGGCTC TGCTGGTTCT 
CCCGGCAAAG ATGGACTCAA TGGTCTCCCA GGCCCCATCG GTCCCCCTGG GCCTCGAGGT 
CGCACTGGTG ATGCTGGTCC TGCTGGTCCT CCCGGCCCTC CTGGACCCCC TGGTCCCCCA 
GGTCCTCCCA GCGGCGGCTA CGACTTGAGC TTCCTGCCCC AGCCACCTCA AGAGAAGGCT 
CACGATGGTG GCCGCTACTA CCGGGCTGAT GATGCCAATG TGGTCCGTGA CCGTGACCTC 
GAGGTGGACA CCACCCTCAA GAGCCTGAGC CAGCAGATCG AGAACATCCG GAGCCCTGAA 
GGCAGCCGCA AGAACCCCGC CCGCACCTGC CGTGACCTCA AGATGTGCCA CTCTGACTGG 
AAGAGCGGAG AATACTGGAT TGACCCCAAC CAAGGCTGCA ACCTGGATGC CATTAAGGTC 
TTCTGCAACA TGGAAACCGG TGAGACCTGT GTATACCCCA CTCAGCCCAG CGTGGCCCAG 
AAGAACTGGT ATATCAGCAA GAACCCCAAG GAAAAGAGGC ACGTCTGGTA CGGCGAGAGC 
ATGACCGGCG GATTCCAGTT CGAGTATGGC GGCCAGGGGT CCGATCCTGC CGATGTGGCC 
ATCCAGCTGA CTTTCCTGCG CCTGATGTCC ACCGAGGCCT CCCAGAACAT CACCTACCAC 
TGCAAGAACA GCGTGGCCTA CATGGACCAG CAGACTGGCA ACCTCAAGAA GGCCCTGCTC 
CTCCAGGGCT CCAACGAGAT CGAGATCCGG GCCGAGGGCA ACAGCCGCTT CACCTACAGC 
GTCACCTACG ATGGCTGCAC GAGTCACACC GGAGCCTGGG GCAAGACAGT GATCGAATAC 
AAAACCACCA AGACCTCCCG CTTGCCCATC ATCGATGTGG CCCCCTTGGA CGTTGGCGCC 
CCAGACCAGG AATTCGGCTT CGACGTTGGC CCTGCCTGCT TCCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL1A1 ( NM_001034039.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001029211.1 Bos taurus collagen type I alpha 1 chain (COL1A1), mRNA.

Price & Availability↑

CloneID	OBa00449
Clone ID Related Accession (Same CDS sequence)	NM_001034039.2
Accession Version	NM_001034039.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	collagen alpha-1(I) chain precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC105184.1. On Aug 31, 2012 this sequence version replaced NM_001034039.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC105184.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGTTCAGCT TTGTGGACCT CCGGCTCCTG CTCCTCTTAG CGGCCACCGC CCTCCTGACG 
CACGGCCAAG AGGAGGGCCA GGAAGAAGGC CAAGAAGAAG ACATCCCACC AGTCACCTGC 
GTACAGAACG GCCTCAGGTA CCATGACCGA GACGTGTGGA AACCCGTGCC CTGCCAGATC 
TGTGTCTGCG ACAACGGCAA CGTGCTGTGC GATGACGTGA TCTGCGACGA ACTTAAGGAC 
TGTCCTAACG CCAAAGTCCC CACGGACGAA TGCTGCCCCG TCTGCCCCGA AGGCCAGGAA 
TCACCCACGG ACCAAGAAAC CACCGGAGTC GAGGGACCGA AAGGAGACAC TGGCCCCCGA 
GGCCCAAGGG GACCCGCCGG CCCCCCCGGC CGAGATGGCA TCCCTGGACA ACCTGGACTT 
CCCGGACCCC CTGGACCCCC CGGACCTCCC GGACCCCCTG GCCTCGGAGG AAACTTTGCT 
CCCCAGTTGT CTTACGGCTA TGATGAGAAA TCAACAGGAA TTTCCGTGCC TGGTCCCATG 
GGTCCTTCTG GTCCTCGTGG TCTCCCTGGC CCCCCTGGCG CACCTGGTCC CCAAGGTTTC 
CAAGGCCCCC CTGGTGAGCC TGGCGAGCCA GGAGCCTCAG GTCCCATGGG TCCCCGTGGT 
CCCCCTGGCC CCCCTGGCAA GAACGGAGAT GATGGCGAAG CTGGAAAGCC TGGTCGTCCT 
GGTGAGCGCG GGCCTCCCGG ACCTCAGGGT GCTCGGGGAT TGCCTGGAAC AGCTGGCCTC 
CCTGGAATGA AGGGACACAG AGGTTTCAGT GGTTTGGATG GTGCCAAGGG AGATGCTGGT 
CCTGCTGGCC CCAAGGGCGA GCCTGGTAGC CCCGGTGAAA ATGGAGCTCC TGGTCAGATG 
GGCCCCCGTG GTCTGCCTGG TGAGAGAGGT CGCCCTGGAG CCCCTGGCCC TGCTGGTGCT 
CGAGGAAATG ATGGTGCGAC TGGTGCTGCT GGGCCCCCTG GTCCCACTGG CCCCGCTGGT 
CCTCCTGGTT TCCCTGGTGC TGTGGGTGCT AAGGGTGAAG GTGGTCCCCA AGGACCCCGA 
GGTTCTGAAG GTCCCCAGGG TGTACGTGGT GAGCCTGGCC CCCCTGGCCC TGCTGGTGCT 
GCTGGCCCTG CTGGCAACCC TGGTGCTGAT GGACAGCCTG GTGCTAAAGG AGCCAATGGC 
GCTCCTGGTA TTGCTGGTGC TCCTGGCTTC CCTGGTGCCC GAGGCCCCTC TGGACCCCAG 
GGCCCCAGCG GCCCCCCTGG CCCCAAGGGT AACAGCGGTG AACCTGGTGC TCCTGGCAGC 
AAAGGAGACA CTGGCGCCAA GGGAGAACCC GGTCCCACTG GTATTCAAGG CCCCCCTGGC 
CCCGCTGGGG AAGAAGGAAA GCGAGGAGCC CGAGGTGAAC CTGGACCTGC TGGCCTGCCT 
GGACCCCCTG GCGAGCGTGG TGGACCTGGA AGCCGTGGTT TCCCTGGCGC CGACGGTGTT 
GCTGGTCCCA AGGGTCCTGC TGGTGAACGC GGTGCTCCTG GCCCTGCTGG CCCCAAAGGT 
TCTCCTGGTG AAGCTGGTCG CCCCGGTGAA GCTGGTCTGC CCGGTGCCAA GGGTCTGACT 
GGAAGCCCTG GCAGCCCGGG TCCTGATGGC AAAACTGGCC CCCCTGGTCC CGCCGGTCAA 
GATGGCCGCC CTGGACCTCC AGGCCCTCCC GGTGCCCGTG GTCAGGCTGG CGTGATGGGT 
TTCCCTGGAC CTAAAGGTGC TGCTGGAGAG CCTGGAAAAG CTGGAGAGCG AGGTGTTCCT 
GGACCCCCTG GCGCTGTTGG TCCTGCTGGC AAAGACGGAG AAGCTGGAGC TCAGGGACCC 
CCAGGACCTG CTGGCCCCGC TGGTGAGAGA GGCGAACAAG GCCCTGCTGG CTCCCCTGGA 
TTCCAGGGTC TCCCCGGCCC TGCTGGTCCT CCTGGTGAAG CAGGCAAACC TGGTGAACAG 
GGTGTTCCTG GAGATCTTGG TGCCCCCGGC CCCTCTGGAG CAAGAGGCGA GAGAGGTTTC 
CCCGGCGAGC GTGGTGTGCA AGGGCCGCCC GGTCCTGCAG GTCCCCGTGG GGCCAATGGT 
GCCCCTGGCA ACGATGGTGC TAAGGGTGAT GCTGGTGCCC CTGGAGCCCC CGGTAGCCAG 
GGTGCCCCTG GCCTTCAAGG AATGCCTGGT GAACGAGGTG CAGCTGGTCT TCCAGGCCCT 
AAGGGTGACA GAGGGGATGC TGGTCCCAAA GGTGCTGATG GTGCTCCTGG CAAAGATGGC 
GTCCGTGGTC TGACTGGTCC CATCGGTCCT CCTGGCCCCG CTGGTGCCCC TGGTGACAAG 
GGTGAAGCTG GTCCTAGTGG CCCAGCCGGT CCCACTGGAG CTCGTGGTGC CCCCGGTGAC 
CGTGGTGAGC CTGGTCCCCC CGGCCCTGCT GGCTTCGCTG GCCCCCCTGG TGCTGATGGC 
CAACCTGGTG CTAAAGGCGA ACCTGGTGAT GCTGGTGCTA AAGGTGACGC TGGTCCCCCC 
GGCCCTGCTG GGCCCGCTGG ACCCCCCGGC CCCATTGGTA ACGTTGGTGC TCCCGGACCC 
AAAGGTGCTC GTGGCAGCGC TGGTCCCCCT GGTGCTACTG GTTTCCCAGG TGCTGCTGGC 
CGAGTCGGTC CCCCCGGCCC CTCTGGAAAT GCTGGACCCC CTGGCCCTCC TGGCCCTGCT 
GGCAAAGAAG GCAGCAAAGG CCCCCGCGGT GAGACTGGCC CCGCTGGGCG TCCCGGTGAA 
GTCGGTCCCC CTGGTCCCCC TGGCCCCGCT GGTGAGAAAG GAGCCCCTGG TGCTGACGGA 
CCTGCTGGAG CTCCTGGCAC TCCTGGACCT CAAGGTATTG CTGGACAGCG TGGTGTGGTC 
GGCCTGCCTG GTCAGAGAGG AGAAAGAGGC TTCCCTGGTC TTCCTGGCCC CTCTGGTGAA 
CCCGGCAAAC AAGGTCCTTC TGGAGCAAGT GGTGAACGTG GCCCCCCTGG TCCCATGGGC 
CCCCCTGGAT TGGCTGGACC CCCTGGCGAG TCTGGACGTG AGGGAGCTCC TGGTGCTGAA 
GGATCCCCTG GACGAGATGG TTCTCCTGGC GCCAAGGGTG ACCGTGGTGA GACCGGCCCT 
GCTGGACCTC CTGGTGCTCC TGGCGCTCCC GGTGCCCCCG GCCCTGTCGG ACCTGCCGGC 
AAGAGCGGTG ATCGTGGTGA GACCGGTCCT GCTGGTCCTG CTGGTCCCAT TGGCCCCGTT 
GGTGCCCGTG GCCCCGCTGG ACCCCAAGGC CCCCGTGGTG ACAAGGGTGA GACAGGCGAA 
CAGGGCGACA GAGGCATTAA GGGTCACCGT GGCTTCTCTG GTCTCCAGGG TCCCCCCGGC 
CCTCCCGGCT CTCCTGGTGA GCAAGGTCCT TCCGGAGCCT CTGGTCCTGC TGGTCCCCGC 
GGTCCCCCTG GCTCTGCTGG TTCTCCCGGC AAAGATGGAC TCAATGGTCT CCCAGGCCCC 
ATCGGTCCCC CTGGGCCTCG AGGTCGCACT GGTGATGCTG GTCCTGCTGG TCCTCCCGGC 
CCTCCTGGAC CCCCTGGTCC CCCAGGTCCT CCCAGCGGCG GCTACGACTT GAGCTTCCTG 
CCCCAGCCAC CTCAAGAGAA GGCTCACGAT GGTGGCCGCT ACTACCGGGC TGATGATGCC 
AATGTGGTCC GTGACCGTGA CCTCGAGGTG GACACCACCC TCAAGAGCCT GAGCCAGCAG 
ATCGAGAACA TCCGGAGCCC TGAAGGCAGC CGCAAGAACC CCGCCCGCAC CTGCCGTGAC 
CTCAAGATGT GCCACTCTGA CTGGAAGAGC GGAGAATACT GGATTGACCC CAACCAAGGC 
TGCAACCTGG ATGCCATTAA GGTCTTCTGC AACATGGAAA CCGGTGAGAC CTGTGTATAC 
CCCACTCAGC CCAGCGTGGC CCAGAAGAAC TGGTATATCA GCAAGAACCC CAAGGAAAAG 
AGGCACGTCT GGTACGGCGA GAGCATGACC GGCGGATTCC AGTTCGAGTA TGGCGGCCAG 
GGGTCCGATC CTGCCGATGT GGCCATCCAG CTGACTTTCC TGCGCCTGAT GTCCACCGAG 
GCCTCCCAGA ACATCACCTA CCACTGCAAG AACAGCGTGG CCTACATGGA CCAGCAGACT 
GGCAACCTCA AGAAGGCCCT GCTCCTCCAG GGCTCCAACG AGATCGAGAT CCGGGCCGAG 
GGCAACAGCC GCTTCACCTA CAGCGTCACC TACGATGGCT GCACGAGTCA CACCGGAGCC 
TGGGGCAAGA CAGTGATCGA ATACAAAACC ACCAAGACCT CCCGCTTGCC CATCATCGAT 
GTGGCCCCCT TGGACGTTGG CGCCCCAGAC CAGGAATTCG GCTTCGACGT TGGCCCTGCC 
TGCTTCCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029211.1
CDS	119..4510
Translation	MFSFVDLRLLLLLAATALLTHGQEEGQEEGQEEDIPPVTCVQNGLRYHDRDVWKPVPCQICVCDNGNVLCDDVICDELKDCPNAKVPTDECCPVCPEGQESPTDQETTGVEGPKGDTGPRGPRGPAGPPGRDGIPGQPGLPGPPGPPGPPGPPGLGGNFAPQLSYGYDEKSTGISVPGPMGPSGPRGLPGPPGAPGPQGFQGPPGEPGEPGASGPMGPRGPPGPPGKNGDDGEAGKPGRPGERGPPGPQGARGLPGTAGLPGMKGHRGFSGLDGAKGDAGPAGPKGEPGSPGENGAPGQMGPRGLPGERGRPGAPGPAGARGNDGATGAAGPPGPTGPAGPPGFPGAVGAKGEGGPQGPRGSEGPQGVRGEPGPPGPAGAAGPAGNPGADGQPGAKGANGAPGIAGAPGFPGARGPSGPQGPSGPPGPKGNSGEPGAPGSKGDTGAKGEPGPTGIQGPPGPAGEEGKRGARGEPGPAGLPGPPGERGGPGSRGFPGADGVAGPKGPAGERGAPGPAGPKGSPGEAGRPGEAGLPGAKGLTGSPGSPGPDGKTGPPGPAGQDGRPGPPGPPGARGQAGVMGFPGPKGAAGEPGKAGERGVPGPPGAVGPAGKDGEAGAQGPPGPAGPAGERGEQGPAGSPGFQGLPGPAGPPGEAGKPGEQGVPGDLGAPGPSGARGERGFPGERGVQGPPGPAGPRGANGAPGNDGAKGDAGAPGAPGSQGAPGLQGMPGERGAAGLPGPKGDRGDAGPKGADGAPGKDGVRGLTGPIGPPGPAGAPGDKGEAGPSGPAGPTGARGAPGDRGEPGPPGPAGFAGPPGADGQPGAKGEPGDAGAKGDAGPPGPAGPAGPPGPIGNVGAPGPKGARGSAGPPGATGFPGAAGRVGPPGPSGNAGPPGPPGPAGKEGSKGPRGETGPAGRPGEVGPPGPPGPAGEKGAPGADGPAGAPGTPGPQGIAGQRGVVGLPGQRGERGFPGLPGPSGEPGKQGPSGASGERGPPGPMGPPGLAGPPGESGREGAPGAEGSPGRDGSPGAKGDRGETGPAGPPGAPGAPGAPGPVGPAGKSGDRGETGPAGPAGPIGPVGARGPAGPQGPRGDKGETGEQGDRGIKGHRGFSGLQGPPGPPGSPGEQGPSGASGPAGPRGPPGSAGSPGKDGLNGLPGPIGPPGPRGRTGDAGPAGPPGPPGPPGPPGPPSGGYDLSFLPQPPQEKAHDGGRYYRADDANVVRDRDLEVDTTLKSLSQQIENIRSPEGSRKNPARTCRDLKMCHSDWKSGEYWIDPNQGCNLDAIKVFCNMETGETCVYPTQPSVAQKNWYISKNPKEKRHVWYGESMTGGFQFEYGGQGSDPADVAIQLTFLRLMSTEASQNITYHCKNSVAYMDQQTGNLKKALLLQGSNEIEIRAEGNSRFTYSVTYDGCTSHTGAWGKTVIEYKTTKTSRLPIIDVAPLDVGAPDQEFGFDVGPACFL

Target ORF information:

RefSeq Version	NM_001034039.2
Organism	Bos taurus(cattle)
Definition	Bos taurus collagen type I alpha 1 chain (COL1A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034039.2

ORF Insert Sequence:

ATGTTCAGCT TTGTGGACCT CCGGCTCCTG CTCCTCTTAG CGGCCACCGC CCTCCTGACG 
CACGGCCAAG AGGAGGGCCA GGAAGAAGGC CAAGAAGAAG ACATCCCACC AGTCACCTGC 
GTACAGAACG GCCTCAGGTA CCATGACCGA GACGTGTGGA AACCCGTGCC CTGCCAGATC 
TGTGTCTGCG ACAACGGCAA CGTGCTGTGC GATGACGTGA TCTGCGACGA ACTTAAGGAC 
TGTCCTAACG CCAAAGTCCC CACGGACGAA TGCTGCCCCG TCTGCCCCGA AGGCCAGGAA 
TCACCCACGG ACCAAGAAAC CACCGGAGTC GAGGGACCGA AAGGAGACAC TGGCCCCCGA 
GGCCCAAGGG GACCCGCCGG CCCCCCCGGC CGAGATGGCA TCCCTGGACA ACCTGGACTT 
CCCGGACCCC CTGGACCCCC CGGACCTCCC GGACCCCCTG GCCTCGGAGG AAACTTTGCT 
CCCCAGTTGT CTTACGGCTA TGATGAGAAA TCAACAGGAA TTTCCGTGCC TGGTCCCATG 
GGTCCTTCTG GTCCTCGTGG TCTCCCTGGC CCCCCTGGCG CACCTGGTCC CCAAGGTTTC 
CAAGGCCCCC CTGGTGAGCC TGGCGAGCCA GGAGCCTCAG GTCCCATGGG TCCCCGTGGT 
CCCCCTGGCC CCCCTGGCAA GAACGGAGAT GATGGCGAAG CTGGAAAGCC TGGTCGTCCT 
GGTGAGCGCG GGCCTCCCGG ACCTCAGGGT GCTCGGGGAT TGCCTGGAAC AGCTGGCCTC 
CCTGGAATGA AGGGACACAG AGGTTTCAGT GGTTTGGATG GTGCCAAGGG AGATGCTGGT 
CCTGCTGGCC CCAAGGGCGA GCCTGGTAGC CCCGGTGAAA ATGGAGCTCC TGGTCAGATG 
GGCCCCCGTG GTCTGCCTGG TGAGAGAGGT CGCCCTGGAG CCCCTGGCCC TGCTGGTGCT 
CGAGGAAATG ATGGTGCGAC TGGTGCTGCT GGGCCCCCTG GTCCCACTGG CCCCGCTGGT 
CCTCCTGGTT TCCCTGGTGC TGTGGGTGCT AAGGGTGAAG GTGGTCCCCA AGGACCCCGA 
GGTTCTGAAG GTCCCCAGGG TGTACGTGGT GAGCCTGGCC CCCCTGGCCC TGCTGGTGCT 
GCTGGCCCTG CTGGCAACCC TGGTGCTGAT GGACAGCCTG GTGCTAAAGG AGCCAATGGC 
GCTCCTGGTA TTGCTGGTGC TCCTGGCTTC CCTGGTGCCC GAGGCCCCTC TGGACCCCAG 
GGCCCCAGCG GCCCCCCTGG CCCCAAGGGT AACAGCGGTG AACCTGGTGC TCCTGGCAGC 
AAAGGAGACA CTGGCGCCAA GGGAGAACCC GGTCCCACTG GTATTCAAGG CCCCCCTGGC 
CCCGCTGGGG AAGAAGGAAA GCGAGGAGCC CGAGGTGAAC CTGGACCTGC TGGCCTGCCT 
GGACCCCCTG GCGAGCGTGG TGGACCTGGA AGCCGTGGTT TCCCTGGCGC CGACGGTGTT 
GCTGGTCCCA AGGGTCCTGC TGGTGAACGC GGTGCTCCTG GCCCTGCTGG CCCCAAAGGT 
TCTCCTGGTG AAGCTGGTCG CCCCGGTGAA GCTGGTCTGC CCGGTGCCAA GGGTCTGACT 
GGAAGCCCTG GCAGCCCGGG TCCTGATGGC AAAACTGGCC CCCCTGGTCC CGCCGGTCAA 
GATGGCCGCC CTGGACCTCC AGGCCCTCCC GGTGCCCGTG GTCAGGCTGG CGTGATGGGT 
TTCCCTGGAC CTAAAGGTGC TGCTGGAGAG CCTGGAAAAG CTGGAGAGCG AGGTGTTCCT 
GGACCCCCTG GCGCTGTTGG TCCTGCTGGC AAAGACGGAG AAGCTGGAGC TCAGGGACCC 
CCAGGACCTG CTGGCCCCGC TGGTGAGAGA GGCGAACAAG GCCCTGCTGG CTCCCCTGGA 
TTCCAGGGTC TCCCCGGCCC TGCTGGTCCT CCTGGTGAAG CAGGCAAACC TGGTGAACAG 
GGTGTTCCTG GAGATCTTGG TGCCCCCGGC CCCTCTGGAG CAAGAGGCGA GAGAGGTTTC 
CCCGGCGAGC GTGGTGTGCA AGGGCCGCCC GGTCCTGCAG GTCCCCGTGG GGCCAATGGT 
GCCCCTGGCA ACGATGGTGC TAAGGGTGAT GCTGGTGCCC CTGGAGCCCC CGGTAGCCAG 
GGTGCCCCTG GCCTTCAAGG AATGCCTGGT GAACGAGGTG CAGCTGGTCT TCCAGGCCCT 
AAGGGTGACA GAGGGGATGC TGGTCCCAAA GGTGCTGATG GTGCTCCTGG CAAAGATGGC 
GTCCGTGGTC TGACTGGTCC CATCGGTCCT CCTGGCCCCG CTGGTGCCCC TGGTGACAAG 
GGTGAAGCTG GTCCTAGTGG CCCAGCCGGT CCCACTGGAG CTCGTGGTGC CCCCGGTGAC 
CGTGGTGAGC CTGGTCCCCC CGGCCCTGCT GGCTTCGCTG GCCCCCCTGG TGCTGATGGC 
CAACCTGGTG CTAAAGGCGA ACCTGGTGAT GCTGGTGCTA AAGGTGACGC TGGTCCCCCC 
GGCCCTGCTG GGCCCGCTGG ACCCCCCGGC CCCATTGGTA ACGTTGGTGC TCCCGGACCC 
AAAGGTGCTC GTGGCAGCGC TGGTCCCCCT GGTGCTACTG GTTTCCCAGG TGCTGCTGGC 
CGAGTCGGTC CCCCCGGCCC CTCTGGAAAT GCTGGACCCC CTGGCCCTCC TGGCCCTGCT 
GGCAAAGAAG GCAGCAAAGG CCCCCGCGGT GAGACTGGCC CCGCTGGGCG TCCCGGTGAA 
GTCGGTCCCC CTGGTCCCCC TGGCCCCGCT GGTGAGAAAG GAGCCCCTGG TGCTGACGGA 
CCTGCTGGAG CTCCTGGCAC TCCTGGACCT CAAGGTATTG CTGGACAGCG TGGTGTGGTC 
GGCCTGCCTG GTCAGAGAGG AGAAAGAGGC TTCCCTGGTC TTCCTGGCCC CTCTGGTGAA 
CCCGGCAAAC AAGGTCCTTC TGGAGCAAGT GGTGAACGTG GCCCCCCTGG TCCCATGGGC 
CCCCCTGGAT TGGCTGGACC CCCTGGCGAG TCTGGACGTG AGGGAGCTCC TGGTGCTGAA 
GGATCCCCTG GACGAGATGG TTCTCCTGGC GCCAAGGGTG ACCGTGGTGA GACCGGCCCT 
GCTGGACCTC CTGGTGCTCC TGGCGCTCCC GGTGCCCCCG GCCCTGTCGG ACCTGCCGGC 
AAGAGCGGTG ATCGTGGTGA GACCGGTCCT GCTGGTCCTG CTGGTCCCAT TGGCCCCGTT 
GGTGCCCGTG GCCCCGCTGG ACCCCAAGGC CCCCGTGGTG ACAAGGGTGA GACAGGCGAA 
CAGGGCGACA GAGGCATTAA GGGTCACCGT GGCTTCTCTG GTCTCCAGGG TCCCCCCGGC 
CCTCCCGGCT CTCCTGGTGA GCAAGGTCCT TCCGGAGCCT CTGGTCCTGC TGGTCCCCGC 
GGTCCCCCTG GCTCTGCTGG TTCTCCCGGC AAAGATGGAC TCAATGGTCT CCCAGGCCCC 
ATCGGTCCCC CTGGGCCTCG AGGTCGCACT GGTGATGCTG GTCCTGCTGG TCCTCCCGGC 
CCTCCTGGAC CCCCTGGTCC CCCAGGTCCT CCCAGCGGCG GCTACGACTT GAGCTTCCTG 
CCCCAGCCAC CTCAAGAGAA GGCTCACGAT GGTGGCCGCT ACTACCGGGC TGATGATGCC 
AATGTGGTCC GTGACCGTGA CCTCGAGGTG GACACCACCC TCAAGAGCCT GAGCCAGCAG 
ATCGAGAACA TCCGGAGCCC TGAAGGCAGC CGCAAGAACC CCGCCCGCAC CTGCCGTGAC 
CTCAAGATGT GCCACTCTGA CTGGAAGAGC GGAGAATACT GGATTGACCC CAACCAAGGC 
TGCAACCTGG ATGCCATTAA GGTCTTCTGC AACATGGAAA CCGGTGAGAC CTGTGTATAC 
CCCACTCAGC CCAGCGTGGC CCAGAAGAAC TGGTATATCA GCAAGAACCC CAAGGAAAAG 
AGGCACGTCT GGTACGGCGA GAGCATGACC GGCGGATTCC AGTTCGAGTA TGGCGGCCAG 
GGGTCCGATC CTGCCGATGT GGCCATCCAG CTGACTTTCC TGCGCCTGAT GTCCACCGAG 
GCCTCCCAGA ACATCACCTA CCACTGCAAG AACAGCGTGG CCTACATGGA CCAGCAGACT 
GGCAACCTCA AGAAGGCCCT GCTCCTCCAG GGCTCCAACG AGATCGAGAT CCGGGCCGAG 
GGCAACAGCC GCTTCACCTA CAGCGTCACC TACGATGGCT GCACGAGTCA CACCGGAGCC 
TGGGGCAAGA CAGTGATCGA ATACAAAACC ACCAAGACCT CCCGCTTGCC CATCATCGAT 
GTGGCCCCCT TGGACGTTGG CGCCCCAGAC CAGGAATTCG GCTTCGACGT TGGCCCTGCC 
TGCTTCCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of Microfibrillar-associated Protein 4 (MFAP4) as a Tropoelastin- and Fibrillin-binding Protein Involved in Elastic Fiber Formation.
The Journal of biological chemistry291(3)1103-14(2016 Jan)
Pilecki B,Holm AT,Schlosser A,Moeller JB,Wohl AP,Zuk AV,Heum?ller SE,Wallis R,Moestrup SK,Sengle G,Holmskov U,Sorensen GL

Developmental Stage-dependent Regulation of Prolyl 3-Hydroxylation in Tendon Type I Collagen.
The Journal of biological chemistry291(2)837-47(2016 Jan)
Taga Y,Kusubata M,Ogawa-Goto K,Hattori S

Snail1 induced in breast cancer cells in 3D collagen I gel environment suppresses cortactin and impairs effective invadopodia formation.
Biochimica et biophysica acta1843(9)2037-54(2014 Sep)
Lee MS,Kim S,Kim BG,Won C,Nam SH,Kang S,Kim HJ,Kang M,Ryu J,Song HE,Lee D,Ye SK,Jeon NL,Kim TY,Cho NH,Lee JW

The aggregation behavior of native collagen in dilute solution studied by intrinsic fluorescence and external probing.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy102186-93(2013 Feb)
Wu K,Liu W,Li G

Two subsystems of meniscal collagen and their different thermal stabilities.
Doklady. Biochemistry and biophysics444175-7(2012 May-Jun)
Ignatieva NY,Zakharkina OL,Lunin VV,Baratova LA,Grokhovskaya TE,Balalaeva IV,Sergeeva EA

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COL1A1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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