LOC106397021 cDNA ORF clone, Brassica napus(rape)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC106397021
Entrez Gene ID	106397021
Full Name	4-coumarate--CoA ligase 2
Synonyms	4CL-D
Gene Type	protein-coding
Organism	Brassica napus(rape)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013837557.2	XP_013693011.2	4-coumarate--CoA ligase 2
XM_013837557.1	XP_013693011.1	4-coumarate--CoA ligase 2-like

Pathways from BioSystems
Gene Ontology

The following LOC106397021 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106397021 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBr243369	XM_013837557.2 Latest version!	Brassica napus 4-coumarate--CoA ligase 2 (LOC106397021), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC106397021 ( XM_013837557.2 ) cDNA ORF clone, Brassica napus(rape) -> XP_013693011.2 Brassica napus 4-coumarate--CoA ligase 2 (LOC106397021), mRNA.

Price & Availability↑

CloneID	OBr243369
Clone ID Related Accession (Same CDS sequence)	XM_013837557.2
Accession Version	XM_013837557.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Brassica napus(rape)
Product	4-coumarate--CoA ligase 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_027770.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 4, 2017 this sequence version replaced XM_013837557.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Brassica napus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 34% of CDS bases ##RefSeq-Attributes-END##

ATGTCTTTAT TTATTACCGT TTCTCGATCA AACAACTTCA ATAGAGAGGA CGTGATACTC 
TGTGTCTTGC CCAAGTTCCA CATCTACGCC CTAAACTCCA TTATGCTATG TAGTCTCAGA 
GTCGGTGCCA CTATTTTGAT CATGCCCAAA TTCGAGATCA CTCTCTTGCT AGAGCAGATC 
CAGAGGTGTA AAGTTACAGT GGCTATGGTC GTGCCACCGA TCGTTTTGGC CATGGTGAAG 
TCTTCGGAGA CGGAGAAGTA TGATCTGACC TCGGTGAGGA TGATTAAGTC AGGTGCGGCT 
CCTCTTGGTA AGGAGCTTGA AGATACCATT AGTGCTAAGT TTCCTAACGC AAGGCTTGGT 
CAGGGCTATG GAATGACGGA GGCAGGTCCG GTGCTAGCAA TGTCACTAGG GTTCGCGAAA 
GAGCCGTTTC CGGTGAAATC AGGAGCATGC GGTACAGTCG TCAGAAACGC CGAGTTGAAG 
ATCGCTGATC CAGACACTGG AAGTTCCCTT CCTAGGAACA GTCCCGGAGA GATATGCATC 
CGCGGCCACC AAATCATGAA AGGCTACCTC AATGACCCGG TGGCCACAGC AGCAACGATA 
GACAAAGAAG GTTGGCTTCA CACCGGAGAT ATTGGATTTG TCGACGATGA CGACGAGCTT 
TTCATTGTCG ATCGCTTGAA AGAGCTTATC AAGTACAAGG GATTCCAGGT GGCTCCGGCG 
GAGCTCGAGT CTCTCCTCAT CTCTCACTCG GACATCAATG ATGTTGCAGT GGTCGCCATG 
AAAGAAGACG ATGCTGGTGA GGTTCCTGTT GCTTTTGTGG TGAGATCCAA AGAGTCAAAT 
TTATCAGAAG ATGAAATTAA GCAATTTGTG TCAAAACAGG TTGTGTTTTA CAAGAGAATC 
AACAAAGTTT TCTTCACTGA CTCCGTTCCT AAAGCTCCAT CAGGGAAGAT ATTGGGGGTA 
ATTGGTAGTT GCTGTAGGTG CTGTCCACGG CCCTATTTAT TTCTACAGCA CCAAATTCAG 
AGCAATCAAG CTTTAAATTT TTTTTTGAAA CCACAGCTAT TGAACTTAGA ATTATCCAAC 
AAGGAGATCT CGCCTGATGA AGCATTGGTG ATTCCTTACT CCTCCGGGAC CACTAGACAT 
CTAAAGGGAG TGATGGAACT CCATGATTTC TTATCATATG TTTTCACTAA ATCTTCTTTT 
CCTAGTAGTT TGTCATCCAT GGTCTCGTCT GTTCCAATTC TTACTGGAAC CAACTTCTCA 
GAATGGAAAG AGAAAGTTGA GTTAACTTTA GGAGTACTAG ACTTGGATTT GGCATTACGT 
GAGGACGAGC CAAATCCACT TACAGAGGAA AGTACCGAAG AAAAGAAAGA ATTCTATAAG 
AATTGGGAAA AAACGAACAG ACTGAGCGTT ATGTTCTTAA GGATGACAAT AGCTAGCAAC 
ATCAAATCTT CCCTTCCACC TGCGAATAAA GCGAATGCTT ATCTGGCAGC TAGAGAAGAA 
CGGTTTAAGA CTGCAGACAA ATCCCTAGCA GGGAAACTTA TGGCAGATCT TACAACCATA 
AAGTATAATG GCACAAGGTC TATGCATGAC ATTGCATTGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013693011.2
CDS	16..1620
Translation	MSLFITVSRSNNFNREDVILCVLPKFHIYALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAMVKSSETEKYDLTSVRMIKSGAAPLGKELEDTISAKFPNARLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAELKIADPDTGSSLPRNSPGEICIRGHQIMKGYLNDPVATAATIDKEGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELESLLISHSDINDVAVVAMKEDDAGEVPVAFVVRSKESNLSEDEIKQFVSKQVVFYKRINKVFFTDSVPKAPSGKILGVIGSCCRCCPRPYLFLQHQIQSNQALNFFLKPQLLNLELSNKEISPDEALVIPYSSGTTRHLKGVMELHDFLSYVFTKSSFPSSLSSMVSSVPILTGTNFSEWKEKVELTLGVLDLDLALREDEPNPLTEESTEEKKEFYKNWEKTNRLSVMFLRMTIASNIKSSLPPANKANAYLAAREERFKTADKSLAGKLMADLTTIKYNGTRSMHDIALK

Target ORF information:

RefSeq Version	XM_013837557.2
Organism	Brassica napus(rape)
Definition	Brassica napus 4-coumarate--CoA ligase 2 (LOC106397021), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013837557.2

ORF Insert Sequence:

ATGTCTTTAT TTATTACCGT TTCTCGATCA AACAACTTCA ATAGAGAGGA CGTGATACTC 
TGTGTCTTGC CCAAGTTCCA CATCTACGCC CTAAACTCCA TTATGCTATG TAGTCTCAGA 
GTCGGTGCCA CTATTTTGAT CATGCCCAAA TTCGAGATCA CTCTCTTGCT AGAGCAGATC 
CAGAGGTGTA AAGTTACAGT GGCTATGGTC GTGCCACCGA TCGTTTTGGC CATGGTGAAG 
TCTTCGGAGA CGGAGAAGTA TGATCTGACC TCGGTGAGGA TGATTAAGTC AGGTGCGGCT 
CCTCTTGGTA AGGAGCTTGA AGATACCATT AGTGCTAAGT TTCCTAACGC AAGGCTTGGT 
CAGGGCTATG GAATGACGGA GGCAGGTCCG GTGCTAGCAA TGTCACTAGG GTTCGCGAAA 
GAGCCGTTTC CGGTGAAATC AGGAGCATGC GGTACAGTCG TCAGAAACGC CGAGTTGAAG 
ATCGCTGATC CAGACACTGG AAGTTCCCTT CCTAGGAACA GTCCCGGAGA GATATGCATC 
CGCGGCCACC AAATCATGAA AGGCTACCTC AATGACCCGG TGGCCACAGC AGCAACGATA 
GACAAAGAAG GTTGGCTTCA CACCGGAGAT ATTGGATTTG TCGACGATGA CGACGAGCTT 
TTCATTGTCG ATCGCTTGAA AGAGCTTATC AAGTACAAGG GATTCCAGGT GGCTCCGGCG 
GAGCTCGAGT CTCTCCTCAT CTCTCACTCG GACATCAATG ATGTTGCAGT GGTCGCCATG 
AAAGAAGACG ATGCTGGTGA GGTTCCTGTT GCTTTTGTGG TGAGATCCAA AGAGTCAAAT 
TTATCAGAAG ATGAAATTAA GCAATTTGTG TCAAAACAGG TTGTGTTTTA CAAGAGAATC 
AACAAAGTTT TCTTCACTGA CTCCGTTCCT AAAGCTCCAT CAGGGAAGAT ATTGGGGGTA 
ATTGGTAGTT GCTGTAGGTG CTGTCCACGG CCCTATTTAT TTCTACAGCA CCAAATTCAG 
AGCAATCAAG CTTTAAATTT TTTTTTGAAA CCACAGCTAT TGAACTTAGA ATTATCCAAC 
AAGGAGATCT CGCCTGATGA AGCATTGGTG ATTCCTTACT CCTCCGGGAC CACTAGACAT 
CTAAAGGGAG TGATGGAACT CCATGATTTC TTATCATATG TTTTCACTAA ATCTTCTTTT 
CCTAGTAGTT TGTCATCCAT GGTCTCGTCT GTTCCAATTC TTACTGGAAC CAACTTCTCA 
GAATGGAAAG AGAAAGTTGA GTTAACTTTA GGAGTACTAG ACTTGGATTT GGCATTACGT 
GAGGACGAGC CAAATCCACT TACAGAGGAA AGTACCGAAG AAAAGAAAGA ATTCTATAAG 
AATTGGGAAA AAACGAACAG ACTGAGCGTT ATGTTCTTAA GGATGACAAT AGCTAGCAAC 
ATCAAATCTT CCCTTCCACC TGCGAATAAA GCGAATGCTT ATCTGGCAGC TAGAGAAGAA 
CGGTTTAAGA CTGCAGACAA ATCCCTAGCA GGGAAACTTA TGGCAGATCT TACAACCATA 
AAGTATAATG GCACAAGGTC TATGCATGAC ATTGCATTGA AATGA