knl-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	knl-1
Entrez Gene ID	176164
Full Name	Kinetochore null protein 1
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_066410.3	NP_498811.1	Kinetochore null protein 1
NM_066410.4	NP_498811.1	Kinetochore null protein 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that microtubule-associated proteins KNL-1 oligomerization may coordinate with other kinetochore activities to ensure the proper organization, function, and sensory capabilities of the kinetochore-microtubule attachment.
Title: The outer kinetochore protein KNL-1 contains a defined oligomerization domain in nematodes.

KNL-1 plays a central role in translating the initiation of kinetochore assembly
Title: KNL-1 directs assembly of the microtubule-binding interface of the kinetochore in C. elegans.

The following knl-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the knl-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa340857	NM_066410.4 Latest version!	Caenorhabditis elegans Kinetochore null protein 1 (knl-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa340857	NM_066410.3	Caenorhabditis elegans Kinetochore null protein 1 (knl-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

knl-1 ( NM_066410.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498811.1 Caenorhabditis elegans Kinetochore null protein 1 (knl-1), partial mRNA.

Price & Availability↑

CloneID	OCa340857
Clone ID Related Accession (Same CDS sequence)	NM_066410.3 , NM_066410.4
Accession Version	NM_066410.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Kinetochore null protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_066410.2. COMPLETENESS: incomplete on both ends.

ATGTCGATGG AGCCTCGTAA GAAGCGGAAC TCGATTCTCA AGGTGCGGCA AGCCGTCGAA 
ACCATCGAGG AAACCGTCAT GAACAGTGGG CCTAGTTCCA CAACAACTAA TCGACGAGTC 
AGCTTTCATA ACGTGAAGCA TGTCAAGCAG TATGACAGGG ACCATGGTAA AATTCTTGAC 
GCCACACCAG TTAAGGAGAA GATTACTGAC ACTATTGGAT CAGATGGTAT TTTGACACCA 
CGTGGCGGAA ACATGGATAT TTCCGAATCT CCGGCCTGCA CGTCCTCATT TCAAGTGTTC 
GGCGGTGGTA ATCTCGATAA AACTATGGAT ATGTCTCTCG AAACAACTAT CAACGAGAAC 
AACGAAACGG CGAGATTGTT TGAAACCACA AGAGATCCAA CACTATTATA CGAAAAGATC 
GTCGAAACCA CAACAAAAGT TACCGAGCGA ATTGTTAGTA TGCCACTGGA TGATACCTTA 
GCAATGTTCA ATACAACGAA TCAAGAAGAT AAGGATATGT CAGTTGATCG TTCAGTTCTT 
TTCACGATTC CCAAAGTTCC GAAGCATAAC GCTACAATGA ATAGAACTAT ACCGATGGAC 
CTCGATGAAT CAAAAGCAGC GGGCGGCCAG TGCGATGAAA CGATGAATGT GTTCAATTTC 
ACAAACTTGG AAGCCGCTGA AATGGATACG AGTAAATTAG ATGAAAATAA TACCATGAAT 
GCTATCCGGA TTCCGATTAA TTCAAACGTC ATGCCTGTAG ACATGGACAT CACTGAACAT 
CACACTTTAA TTGAAGAAAA GAAAAATGAT ACATTCGGGC CAAGTCAACT GATGGACATT 
TCGGCGCCAC AAGTTCAAGT TAATGATACT TTGGCCATTT TCAACAGTCC GAGAGACATC 
TGTAATAAGG GTTTGGGTGT TCCTCAGAAT CTAATAAATA TCGCCTCGAA CGTCGTACCT 
GTGGACATGG ACATCACTGA TCAGGCCGTA TTAAACGCGG AGAAGAAAAA TGATCAATTC 
GAGACAAGTC AGCTTATGGA CATTTCTATT CCGAAAGTTC TAGTAAATGA CACTATGGCG 
ATGTTCAACA GCCCGAAACA CGTCAGTAAG AGCAGCATGG ATCTCGAGAA AACGATTGAA 
GCCGCTGACA AATCAACGAA ATACCCGAGT ATCGCAGATG AGGTGGAAGA TTTAGACATG 
GATATGGATA TCACTGAACA ACAACCATGT GAGGCTGGTA ATCAGCAGAA CGACGGCTTG 
CAACTTCAAA AGGAGGATTT AATGGACATT TCGGTGATTC GAGATTCACC TGCAGTAAAC 
GACACCATGG CTGTGTTCCA GAGTCCTGCC AGAGTAAAGA TCGGAGCGAA CAACTCGATC 
ATTGATTCGC AGAAATCTAT CGTGTTCGGT GACGAAATGA GCATTGACGA GACACAAAAT 
GATGGAACCT TGACGTTGCC AAAGTCGAAT GTAGAAGTGA CTACAACTAA TGATGTCTAC 
ACGTCTCTCG AGCGGCAAGA GGAAAATGCT TCAGAAAACG TATCCATGAT AAACGAATCT 
TCTGTTCATT CGGAAATCGA CAAAAAGTCG TTTATGCTCA TCGAAGAAGA AAGGGCTTTT 
ATGCACTCCT CCATGATTGA TGTAGCACAA AAGTTGGAAG ACGATGGTTC GTCGAAGACG 
CCAGTCATCC TTGCTTCACA GTCAGCTTCT CTTGCCACTA AAGAACCATC AGCCCTTCAC 
AACTCGAGTG CAACTCTCAA CAATTCGATG GAATTGGACA ACAATACTCT TCTTAAAACT 
ATGCAAATTA CAACGTGTGA AGACATTAGC ATGGTCCATG AGTCTATTGC TGTTGAACTG 
AACAGTAACA AAGAGCAGGA GCAATTCGGA GATGAGACTT TGCAGAAAAA TGATACCTCG 
AATACTGGCG CGAATTTCAC ATTCCAAGGC CATAATGAAA CATCGCAAAT CATGAACAAT 
GTCGACTCGG AAGCAGTGAA CACGTCCAAG ATTTCAACAT ATTCGGCTTT CAATTTGAGC 
ATCAACCAGT CTATCTCTAA ACGACGTCGA TCTCTTCTGA ATTCTGCTCG TGAATCTCCT 
CGTCGTGTTG CGTTGGAGAA TTCTATAATG TCGATGAATG GGCAAACAAT GGAAGCTCTG 
ACAGAATATC GACAGAATAA AACTATGCAG ACGAGTCAAG ATTCGATGCC GAGTATGAGT 
TTGAACGATT CGGGAAGAGA TATTCTCGCG ATGAATACAT CAGTCCGCTC TCCTCATCTG 
AATTCTTCAA AAACTGCTGC CCCAGGAACA CCATCATTGA TGTCACAAAA TGTACAACTT 
CCACCTCCAT CTCCTCAATT CGAAATGCCA GACTTCGATC CAGCTGTGGT CAACGTTGTA 
TATTTAACAT CTGAAGATCC GTCCACTGAA CAACATCCAG AAGCTCTCAA ATTTCAGCGT 
ATTGTTGAAA ACGAGAAAAT GAAAGTACAA CACGAGATTG ATTCTCTGAA TTCAACCAAT 
CAACTTTCTG CTGAGAAAAT TGATATGTTG AAGACTAAGG AGCTCTTGAA GTTTAGTCAT 
GATGAGCGAG AAGCGATTAT GATTGCAAGA AAAGACGCGG AAATCAAGTT TTTGGAGCTT 
CGTCTGAAAT TTGCACTCGA GAAAAAAATT GAAAGTGACC AGGAAATTGC TGAACTAGAA 
CAAGGAAATT CGAAAATGGC TGAGCAGCTA AGAGGTCTCG ATAAGATGGC TGTCGTTCAA 
AAAGAACTAG AAAAGCTGAG AAGTCTTCCT CCATCACGCG AAGAGAGCGG GAAAATCCGA 
AAGGAGTGGA TGGAGATGAA GCAATGGGAA TTCGACCAGA AAATGAAAGC ACTCCGAAAT 
GTACGCTCAA ACATGATTGC ACTTCGTTCA GAGAAAAATG CTCTCGAAAT GAAAGTCGCG 
GAAGAACACG AGAAGTTTGC CCAGAGGAAC GATTTGAAGA AAAGTCGAAT GCTGGTGTTC 
TCTAAGGCTG TTAAGAAAAT TGTGAACTTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498811.1
CDS	1..3033
Translation	MSMEPRKKRNSILKVRQAVETIEETVMNSGPSSTTTNRRVSFHNVKHVKQYDRDHGKILDATPVKEKITDTIGSDGILTPRGGNMDISESPACTSSFQVFGGGNLDKTMDMSLETTINENNETARLFETTRDPTLLYEKIVETTTKVTERIVSMPLDDTLAMFNTTNQEDKDMSVDRSVLFTIPKVPKHNATMNRTIPMDLDESKAAGGQCDETMNVFNFTNLEAAEMDTSKLDENNTMNAIRIPINSNVMPVDMDITEHHTLIEEKKNDTFGPSQLMDISAPQVQVNDTLAIFNSPRDICNKGLGVPQNLINIASNVVPVDMDITDQAVLNAEKKNDQFETSQLMDISIPKVLVNDTMAMFNSPKHVSKSSMDLEKTIEAADKSTKYPSIADEVEDLDMDMDITEQQPCEAGNQQNDGLQLQKEDLMDISVIRDSPAVNDTMAVFQSPARVKIGANNSIIDSQKSIVFGDEMSIDETQNDGTLTLPKSNVEVTTTNDVYTSLERQEENASENVSMINESSVHSEIDKKSFMLIEEERAFMHSSMIDVAQKLEDDGSSKTPVILASQSASLATKEPSALHNSSATLNNSMELDNNTLLKTMQITTCEDISMVHESIAVELNSNKEQEQFGDETLQKNDTSNTGANFTFQGHNETSQIMNNVDSEAVNTSKISTYSAFNLSINQSISKRRRSLLNSARESPRRVALENSIMSMNGQTMEALTEYRQNKTMQTSQDSMPSMSLNDSGRDILAMNTSVRSPHLNSSKTAAPGTPSLMSQNVQLPPPSPQFEMPDFDPAVVNVVYLTSEDPSTEQHPEALKFQRIVENEKMKVQHEIDSLNSTNQLSAEKIDMLKTKELLKFSHDEREAIMIARKDAEIKFLELRLKFALEKKIESDQEIAELEQGNSKMAEQLRGLDKMAVVQKELEKLRSLPPSREESGKIRKEWMEMKQWEFDQKMKALRNVRSNMIALRSEKNALEMKVAEEHEKFAQRNDLKKSRMLVFSKAVKKIVNF

Target ORF information:

RefSeq Version	NM_066410.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Kinetochore null protein 1 (knl-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066410.3

ORF Insert Sequence:

ATGTCGATGG AGCCTCGTAA GAAGCGGAAC TCGATTCTCA AGGTGCGGCA AGCCGTCGAA 
ACCATCGAGG AAACCGTCAT GAACAGTGGG CCTAGTTCCA CAACAACTAA TCGACGAGTC 
AGCTTTCATA ACGTGAAGCA TGTCAAGCAG TATGACAGGG ACCATGGTAA AATTCTTGAC 
GCCACACCAG TTAAGGAGAA GATTACTGAC ACTATTGGAT CAGATGGTAT TTTGACACCA 
CGTGGCGGAA ACATGGATAT TTCCGAATCT CCGGCCTGCA CGTCCTCATT TCAAGTGTTC 
GGCGGTGGTA ATCTCGATAA AACTATGGAT ATGTCTCTCG AAACAACTAT CAACGAGAAC 
AACGAAACGG CGAGATTGTT TGAAACCACA AGAGATCCAA CACTATTATA CGAAAAGATC 
GTCGAAACCA CAACAAAAGT TACCGAGCGA ATTGTTAGTA TGCCACTGGA TGATACCTTA 
GCAATGTTCA ATACAACGAA TCAAGAAGAT AAGGATATGT CAGTTGATCG TTCAGTTCTT 
TTCACGATTC CCAAAGTTCC GAAGCATAAC GCTACAATGA ATAGAACTAT ACCGATGGAC 
CTCGATGAAT CAAAAGCAGC GGGCGGCCAG TGCGATGAAA CGATGAATGT GTTCAATTTC 
ACAAACTTGG AAGCCGCTGA AATGGATACG AGTAAATTAG ATGAAAATAA TACCATGAAT 
GCTATCCGGA TTCCGATTAA TTCAAACGTC ATGCCTGTAG ACATGGACAT CACTGAACAT 
CACACTTTAA TTGAAGAAAA GAAAAATGAT ACATTCGGGC CAAGTCAACT GATGGACATT 
TCGGCGCCAC AAGTTCAAGT TAATGATACT TTGGCCATTT TCAACAGTCC GAGAGACATC 
TGTAATAAGG GTTTGGGTGT TCCTCAGAAT CTAATAAATA TCGCCTCGAA CGTCGTACCT 
GTGGACATGG ACATCACTGA TCAGGCCGTA TTAAACGCGG AGAAGAAAAA TGATCAATTC 
GAGACAAGTC AGCTTATGGA CATTTCTATT CCGAAAGTTC TAGTAAATGA CACTATGGCG 
ATGTTCAACA GCCCGAAACA CGTCAGTAAG AGCAGCATGG ATCTCGAGAA AACGATTGAA 
GCCGCTGACA AATCAACGAA ATACCCGAGT ATCGCAGATG AGGTGGAAGA TTTAGACATG 
GATATGGATA TCACTGAACA ACAACCATGT GAGGCTGGTA ATCAGCAGAA CGACGGCTTG 
CAACTTCAAA AGGAGGATTT AATGGACATT TCGGTGATTC GAGATTCACC TGCAGTAAAC 
GACACCATGG CTGTGTTCCA GAGTCCTGCC AGAGTAAAGA TCGGAGCGAA CAACTCGATC 
ATTGATTCGC AGAAATCTAT CGTGTTCGGT GACGAAATGA GCATTGACGA GACACAAAAT 
GATGGAACCT TGACGTTGCC AAAGTCGAAT GTAGAAGTGA CTACAACTAA TGATGTCTAC 
ACGTCTCTCG AGCGGCAAGA GGAAAATGCT TCAGAAAACG TATCCATGAT AAACGAATCT 
TCTGTTCATT CGGAAATCGA CAAAAAGTCG TTTATGCTCA TCGAAGAAGA AAGGGCTTTT 
ATGCACTCCT CCATGATTGA TGTAGCACAA AAGTTGGAAG ACGATGGTTC GTCGAAGACG 
CCAGTCATCC TTGCTTCACA GTCAGCTTCT CTTGCCACTA AAGAACCATC AGCCCTTCAC 
AACTCGAGTG CAACTCTCAA CAATTCGATG GAATTGGACA ACAATACTCT TCTTAAAACT 
ATGCAAATTA CAACGTGTGA AGACATTAGC ATGGTCCATG AGTCTATTGC TGTTGAACTG 
AACAGTAACA AAGAGCAGGA GCAATTCGGA GATGAGACTT TGCAGAAAAA TGATACCTCG 
AATACTGGCG CGAATTTCAC ATTCCAAGGC CATAATGAAA CATCGCAAAT CATGAACAAT 
GTCGACTCGG AAGCAGTGAA CACGTCCAAG ATTTCAACAT ATTCGGCTTT CAATTTGAGC 
ATCAACCAGT CTATCTCTAA ACGACGTCGA TCTCTTCTGA ATTCTGCTCG TGAATCTCCT 
CGTCGTGTTG CGTTGGAGAA TTCTATAATG TCGATGAATG GGCAAACAAT GGAAGCTCTG 
ACAGAATATC GACAGAATAA AACTATGCAG ACGAGTCAAG ATTCGATGCC GAGTATGAGT 
TTGAACGATT CGGGAAGAGA TATTCTCGCG ATGAATACAT CAGTCCGCTC TCCTCATCTG 
AATTCTTCAA AAACTGCTGC CCCAGGAACA CCATCATTGA TGTCACAAAA TGTACAACTT 
CCACCTCCAT CTCCTCAATT CGAAATGCCA GACTTCGATC CAGCTGTGGT CAACGTTGTA 
TATTTAACAT CTGAAGATCC GTCCACTGAA CAACATCCAG AAGCTCTCAA ATTTCAGCGT 
ATTGTTGAAA ACGAGAAAAT GAAAGTACAA CACGAGATTG ATTCTCTGAA TTCAACCAAT 
CAACTTTCTG CTGAGAAAAT TGATATGTTG AAGACTAAGG AGCTCTTGAA GTTTAGTCAT 
GATGAGCGAG AAGCGATTAT GATTGCAAGA AAAGACGCGG AAATCAAGTT TTTGGAGCTT 
CGTCTGAAAT TTGCACTCGA GAAAAAAATT GAAAGTGACC AGGAAATTGC TGAACTAGAA 
CAAGGAAATT CGAAAATGGC TGAGCAGCTA AGAGGTCTCG ATAAGATGGC TGTCGTTCAA 
AAAGAACTAG AAAAGCTGAG AAGTCTTCCT CCATCACGCG AAGAGAGCGG GAAAATCCGA 
AAGGAGTGGA TGGAGATGAA GCAATGGGAA TTCGACCAGA AAATGAAAGC ACTCCGAAAT 
GTACGCTCAA ACATGATTGC ACTTCGTTCA GAGAAAAATG CTCTCGAAAT GAAAGTCGCG 
GAAGAACACG AGAAGTTTGC CCAGAGGAAC GATTTGAAGA AAAGTCGAAT GCTGGTGTTC 
TCTAAGGCTG TTAAGAAAAT TGTGAACTTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

knl-1 ( NM_066410.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498811.1 Caenorhabditis elegans Kinetochore null protein 1 (knl-1), partial mRNA.

Price & Availability↑

CloneID	OCa340857
Clone ID Related Accession (Same CDS sequence)	NM_066410.3 , NM_066410.4
Accession Version	NM_066410.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Kinetochore null protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_066410.3. COMPLETENESS: incomplete on the 5' end.

ATGTCGATGG AGCCTCGTAA GAAGCGGAAC TCGATTCTCA AGGTGCGGCA AGCCGTCGAA 
ACCATCGAGG AAACCGTCAT GAACAGTGGG CCTAGTTCCA CAACAACTAA TCGACGAGTC 
AGCTTTCATA ACGTGAAGCA TGTCAAGCAG TATGACAGGG ACCATGGTAA AATTCTTGAC 
GCCACACCAG TTAAGGAGAA GATTACTGAC ACTATTGGAT CAGATGGTAT TTTGACACCA 
CGTGGCGGAA ACATGGATAT TTCCGAATCT CCGGCCTGCA CGTCCTCATT TCAAGTGTTC 
GGCGGTGGTA ATCTCGATAA AACTATGGAT ATGTCTCTCG AAACAACTAT CAACGAGAAC 
AACGAAACGG CGAGATTGTT TGAAACCACA AGAGATCCAA CACTATTATA CGAAAAGATC 
GTCGAAACCA CAACAAAAGT TACCGAGCGA ATTGTTAGTA TGCCACTGGA TGATACCTTA 
GCAATGTTCA ATACAACGAA TCAAGAAGAT AAGGATATGT CAGTTGATCG TTCAGTTCTT 
TTCACGATTC CCAAAGTTCC GAAGCATAAC GCTACAATGA ATAGAACTAT ACCGATGGAC 
CTCGATGAAT CAAAAGCAGC GGGCGGCCAG TGCGATGAAA CGATGAATGT GTTCAATTTC 
ACAAACTTGG AAGCCGCTGA AATGGATACG AGTAAATTAG ATGAAAATAA TACCATGAAT 
GCTATCCGGA TTCCGATTAA TTCAAACGTC ATGCCTGTAG ACATGGACAT CACTGAACAT 
CACACTTTAA TTGAAGAAAA GAAAAATGAT ACATTCGGGC CAAGTCAACT GATGGACATT 
TCGGCGCCAC AAGTTCAAGT TAATGATACT TTGGCCATTT TCAACAGTCC GAGAGACATC 
TGTAATAAGG GTTTGGGTGT TCCTCAGAAT CTAATAAATA TCGCCTCGAA CGTCGTACCT 
GTGGACATGG ACATCACTGA TCAGGCCGTA TTAAACGCGG AGAAGAAAAA TGATCAATTC 
GAGACAAGTC AGCTTATGGA CATTTCTATT CCGAAAGTTC TAGTAAATGA CACTATGGCG 
ATGTTCAACA GCCCGAAACA CGTCAGTAAG AGCAGCATGG ATCTCGAGAA AACGATTGAA 
GCCGCTGACA AATCAACGAA ATACCCGAGT ATCGCAGATG AGGTGGAAGA TTTAGACATG 
GATATGGATA TCACTGAACA ACAACCATGT GAGGCTGGTA ATCAGCAGAA CGACGGCTTG 
CAACTTCAAA AGGAGGATTT AATGGACATT TCGGTGATTC GAGATTCACC TGCAGTAAAC 
GACACCATGG CTGTGTTCCA GAGTCCTGCC AGAGTAAAGA TCGGAGCGAA CAACTCGATC 
ATTGATTCGC AGAAATCTAT CGTGTTCGGT GACGAAATGA GCATTGACGA GACACAAAAT 
GATGGAACCT TGACGTTGCC AAAGTCGAAT GTAGAAGTGA CTACAACTAA TGATGTCTAC 
ACGTCTCTCG AGCGGCAAGA GGAAAATGCT TCAGAAAACG TATCCATGAT AAACGAATCT 
TCTGTTCATT CGGAAATCGA CAAAAAGTCG TTTATGCTCA TCGAAGAAGA AAGGGCTTTT 
ATGCACTCCT CCATGATTGA TGTAGCACAA AAGTTGGAAG ACGATGGTTC GTCGAAGACG 
CCAGTCATCC TTGCTTCACA GTCAGCTTCT CTTGCCACTA AAGAACCATC AGCCCTTCAC 
AACTCGAGTG CAACTCTCAA CAATTCGATG GAATTGGACA ACAATACTCT TCTTAAAACT 
ATGCAAATTA CAACGTGTGA AGACATTAGC ATGGTCCATG AGTCTATTGC TGTTGAACTG 
AACAGTAACA AAGAGCAGGA GCAATTCGGA GATGAGACTT TGCAGAAAAA TGATACCTCG 
AATACTGGCG CGAATTTCAC ATTCCAAGGC CATAATGAAA CATCGCAAAT CATGAACAAT 
GTCGACTCGG AAGCAGTGAA CACGTCCAAG ATTTCAACAT ATTCGGCTTT CAATTTGAGC 
ATCAACCAGT CTATCTCTAA ACGACGTCGA TCTCTTCTGA ATTCTGCTCG TGAATCTCCT 
CGTCGTGTTG CGTTGGAGAA TTCTATAATG TCGATGAATG GGCAAACAAT GGAAGCTCTG 
ACAGAATATC GACAGAATAA AACTATGCAG ACGAGTCAAG ATTCGATGCC GAGTATGAGT 
TTGAACGATT CGGGAAGAGA TATTCTCGCG ATGAATACAT CAGTCCGCTC TCCTCATCTG 
AATTCTTCAA AAACTGCTGC CCCAGGAACA CCATCATTGA TGTCACAAAA TGTACAACTT 
CCACCTCCAT CTCCTCAATT CGAAATGCCA GACTTCGATC CAGCTGTGGT CAACGTTGTA 
TATTTAACAT CTGAAGATCC GTCCACTGAA CAACATCCAG AAGCTCTCAA ATTTCAGCGT 
ATTGTTGAAA ACGAGAAAAT GAAAGTACAA CACGAGATTG ATTCTCTGAA TTCAACCAAT 
CAACTTTCTG CTGAGAAAAT TGATATGTTG AAGACTAAGG AGCTCTTGAA GTTTAGTCAT 
GATGAGCGAG AAGCGATTAT GATTGCAAGA AAAGACGCGG AAATCAAGTT TTTGGAGCTT 
CGTCTGAAAT TTGCACTCGA GAAAAAAATT GAAAGTGACC AGGAAATTGC TGAACTAGAA 
CAAGGAAATT CGAAAATGGC TGAGCAGCTA AGAGGTCTCG ATAAGATGGC TGTCGTTCAA 
AAAGAACTAG AAAAGCTGAG AAGTCTTCCT CCATCACGCG AAGAGAGCGG GAAAATCCGA 
AAGGAGTGGA TGGAGATGAA GCAATGGGAA TTCGACCAGA AAATGAAAGC ACTCCGAAAT 
GTACGCTCAA ACATGATTGC ACTTCGTTCA GAGAAAAATG CTCTCGAAAT GAAAGTCGCG 
GAAGAACACG AGAAGTTTGC CCAGAGGAAC GATTTGAAGA AAAGTCGAAT GCTGGTGTTC 
TCTAAGGCTG TTAAGAAAAT TGTGAACTTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498811.1
CDS	1..3033
Translation	MSMEPRKKRNSILKVRQAVETIEETVMNSGPSSTTTNRRVSFHNVKHVKQYDRDHGKILDATPVKEKITDTIGSDGILTPRGGNMDISESPACTSSFQVFGGGNLDKTMDMSLETTINENNETARLFETTRDPTLLYEKIVETTTKVTERIVSMPLDDTLAMFNTTNQEDKDMSVDRSVLFTIPKVPKHNATMNRTIPMDLDESKAAGGQCDETMNVFNFTNLEAAEMDTSKLDENNTMNAIRIPINSNVMPVDMDITEHHTLIEEKKNDTFGPSQLMDISAPQVQVNDTLAIFNSPRDICNKGLGVPQNLINIASNVVPVDMDITDQAVLNAEKKNDQFETSQLMDISIPKVLVNDTMAMFNSPKHVSKSSMDLEKTIEAADKSTKYPSIADEVEDLDMDMDITEQQPCEAGNQQNDGLQLQKEDLMDISVIRDSPAVNDTMAVFQSPARVKIGANNSIIDSQKSIVFGDEMSIDETQNDGTLTLPKSNVEVTTTNDVYTSLERQEENASENVSMINESSVHSEIDKKSFMLIEEERAFMHSSMIDVAQKLEDDGSSKTPVILASQSASLATKEPSALHNSSATLNNSMELDNNTLLKTMQITTCEDISMVHESIAVELNSNKEQEQFGDETLQKNDTSNTGANFTFQGHNETSQIMNNVDSEAVNTSKISTYSAFNLSINQSISKRRRSLLNSARESPRRVALENSIMSMNGQTMEALTEYRQNKTMQTSQDSMPSMSLNDSGRDILAMNTSVRSPHLNSSKTAAPGTPSLMSQNVQLPPPSPQFEMPDFDPAVVNVVYLTSEDPSTEQHPEALKFQRIVENEKMKVQHEIDSLNSTNQLSAEKIDMLKTKELLKFSHDEREAIMIARKDAEIKFLELRLKFALEKKIESDQEIAELEQGNSKMAEQLRGLDKMAVVQKELEKLRSLPPSREESGKIRKEWMEMKQWEFDQKMKALRNVRSNMIALRSEKNALEMKVAEEHEKFAQRNDLKKSRMLVFSKAVKKIVNF

Target ORF information:

RefSeq Version	NM_066410.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Kinetochore null protein 1 (knl-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066410.4

ORF Insert Sequence:

ATGTCGATGG AGCCTCGTAA GAAGCGGAAC TCGATTCTCA AGGTGCGGCA AGCCGTCGAA 
ACCATCGAGG AAACCGTCAT GAACAGTGGG CCTAGTTCCA CAACAACTAA TCGACGAGTC 
AGCTTTCATA ACGTGAAGCA TGTCAAGCAG TATGACAGGG ACCATGGTAA AATTCTTGAC 
GCCACACCAG TTAAGGAGAA GATTACTGAC ACTATTGGAT CAGATGGTAT TTTGACACCA 
CGTGGCGGAA ACATGGATAT TTCCGAATCT CCGGCCTGCA CGTCCTCATT TCAAGTGTTC 
GGCGGTGGTA ATCTCGATAA AACTATGGAT ATGTCTCTCG AAACAACTAT CAACGAGAAC 
AACGAAACGG CGAGATTGTT TGAAACCACA AGAGATCCAA CACTATTATA CGAAAAGATC 
GTCGAAACCA CAACAAAAGT TACCGAGCGA ATTGTTAGTA TGCCACTGGA TGATACCTTA 
GCAATGTTCA ATACAACGAA TCAAGAAGAT AAGGATATGT CAGTTGATCG TTCAGTTCTT 
TTCACGATTC CCAAAGTTCC GAAGCATAAC GCTACAATGA ATAGAACTAT ACCGATGGAC 
CTCGATGAAT CAAAAGCAGC GGGCGGCCAG TGCGATGAAA CGATGAATGT GTTCAATTTC 
ACAAACTTGG AAGCCGCTGA AATGGATACG AGTAAATTAG ATGAAAATAA TACCATGAAT 
GCTATCCGGA TTCCGATTAA TTCAAACGTC ATGCCTGTAG ACATGGACAT CACTGAACAT 
CACACTTTAA TTGAAGAAAA GAAAAATGAT ACATTCGGGC CAAGTCAACT GATGGACATT 
TCGGCGCCAC AAGTTCAAGT TAATGATACT TTGGCCATTT TCAACAGTCC GAGAGACATC 
TGTAATAAGG GTTTGGGTGT TCCTCAGAAT CTAATAAATA TCGCCTCGAA CGTCGTACCT 
GTGGACATGG ACATCACTGA TCAGGCCGTA TTAAACGCGG AGAAGAAAAA TGATCAATTC 
GAGACAAGTC AGCTTATGGA CATTTCTATT CCGAAAGTTC TAGTAAATGA CACTATGGCG 
ATGTTCAACA GCCCGAAACA CGTCAGTAAG AGCAGCATGG ATCTCGAGAA AACGATTGAA 
GCCGCTGACA AATCAACGAA ATACCCGAGT ATCGCAGATG AGGTGGAAGA TTTAGACATG 
GATATGGATA TCACTGAACA ACAACCATGT GAGGCTGGTA ATCAGCAGAA CGACGGCTTG 
CAACTTCAAA AGGAGGATTT AATGGACATT TCGGTGATTC GAGATTCACC TGCAGTAAAC 
GACACCATGG CTGTGTTCCA GAGTCCTGCC AGAGTAAAGA TCGGAGCGAA CAACTCGATC 
ATTGATTCGC AGAAATCTAT CGTGTTCGGT GACGAAATGA GCATTGACGA GACACAAAAT 
GATGGAACCT TGACGTTGCC AAAGTCGAAT GTAGAAGTGA CTACAACTAA TGATGTCTAC 
ACGTCTCTCG AGCGGCAAGA GGAAAATGCT TCAGAAAACG TATCCATGAT AAACGAATCT 
TCTGTTCATT CGGAAATCGA CAAAAAGTCG TTTATGCTCA TCGAAGAAGA AAGGGCTTTT 
ATGCACTCCT CCATGATTGA TGTAGCACAA AAGTTGGAAG ACGATGGTTC GTCGAAGACG 
CCAGTCATCC TTGCTTCACA GTCAGCTTCT CTTGCCACTA AAGAACCATC AGCCCTTCAC 
AACTCGAGTG CAACTCTCAA CAATTCGATG GAATTGGACA ACAATACTCT TCTTAAAACT 
ATGCAAATTA CAACGTGTGA AGACATTAGC ATGGTCCATG AGTCTATTGC TGTTGAACTG 
AACAGTAACA AAGAGCAGGA GCAATTCGGA GATGAGACTT TGCAGAAAAA TGATACCTCG 
AATACTGGCG CGAATTTCAC ATTCCAAGGC CATAATGAAA CATCGCAAAT CATGAACAAT 
GTCGACTCGG AAGCAGTGAA CACGTCCAAG ATTTCAACAT ATTCGGCTTT CAATTTGAGC 
ATCAACCAGT CTATCTCTAA ACGACGTCGA TCTCTTCTGA ATTCTGCTCG TGAATCTCCT 
CGTCGTGTTG CGTTGGAGAA TTCTATAATG TCGATGAATG GGCAAACAAT GGAAGCTCTG 
ACAGAATATC GACAGAATAA AACTATGCAG ACGAGTCAAG ATTCGATGCC GAGTATGAGT 
TTGAACGATT CGGGAAGAGA TATTCTCGCG ATGAATACAT CAGTCCGCTC TCCTCATCTG 
AATTCTTCAA AAACTGCTGC CCCAGGAACA CCATCATTGA TGTCACAAAA TGTACAACTT 
CCACCTCCAT CTCCTCAATT CGAAATGCCA GACTTCGATC CAGCTGTGGT CAACGTTGTA 
TATTTAACAT CTGAAGATCC GTCCACTGAA CAACATCCAG AAGCTCTCAA ATTTCAGCGT 
ATTGTTGAAA ACGAGAAAAT GAAAGTACAA CACGAGATTG ATTCTCTGAA TTCAACCAAT 
CAACTTTCTG CTGAGAAAAT TGATATGTTG AAGACTAAGG AGCTCTTGAA GTTTAGTCAT 
GATGAGCGAG AAGCGATTAT GATTGCAAGA AAAGACGCGG AAATCAAGTT TTTGGAGCTT 
CGTCTGAAAT TTGCACTCGA GAAAAAAATT GAAAGTGACC AGGAAATTGC TGAACTAGAA 
CAAGGAAATT CGAAAATGGC TGAGCAGCTA AGAGGTCTCG ATAAGATGGC TGTCGTTCAA 
AAAGAACTAG AAAAGCTGAG AAGTCTTCCT CCATCACGCG AAGAGAGCGG GAAAATCCGA 
AAGGAGTGGA TGGAGATGAA GCAATGGGAA TTCGACCAGA AAATGAAAGC ACTCCGAAAT 
GTACGCTCAA ACATGATTGC ACTTCGTTCA GAGAAAAATG CTCTCGAAAT GAAAGTCGCG 
GAAGAACACG AGAAGTTTGC CCAGAGGAAC GATTTGAAGA AAAGTCGAAT GCTGGTGTTC 
TCTAAGGCTG TTAAGAAAAT TGTGAACTTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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knl-1 cDNA ORF clone, Caenorhabditis elegans

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