let-2 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	let-2
Entrez Gene ID	181708
Full Name	Collagen alpha-2(IV) chain
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_078262.4	NP_510663.1	Collagen alpha-2(IV) chain
NM_078263.4	NP_510664.1	Collagen alpha-2(IV) chain
NM_078262.5	NP_510663.1	Collagen alpha-2(IV) chain
NM_078263.3	NP_510664.1	Collagen alpha-2(IV) chain

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	COL4A2	XP_001136859.1
Canis lupus familiaris (dog)	COL4A2	XP_534183.3
Rattus norvegicus (Norway rat)	Col4a2	XP_006253538.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009200	XP_319976.3
	COL4A2	XP_002800878.1
Drosophila melanogaster (fruit fly)	Cg25C	NP_723046.1
Caenorhabditis elegans (roundworm)	emb-9	NP_001022662.1
Bos taurus (cattle)	COL4A2	XP_002692057.2
Mus musculus (house mouse)	Col4a2	NP_034062.3
Gallus gallus (chicken)	COL4A2	NP_001155862.1
Danio rerio (zebrafish)	col4a2	XP_687811.6
Caenorhabditis elegans (roundworm)	let-2	NP_510663.1
Xenopus tropicalis (tropical clawed frog)	col4a2	XP_004912045.1
Homo sapiens (human)	COL4A2	NP_001837.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

FBL-1C and LET-2 act downstream of MIG-17-dependent proteolysis to recruit NID-1 and that LET-2 also activates a NID-1-independent pathway, thereby inducing the remodeling of the BM required for directional control of leader cell migration.
Title: MIG-17/ADAMTS controls cell migration by recruiting nidogen to the basement membrane in C. elegans.

The following let-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the let-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa349689	NM_078263.4 Latest version!	Caenorhabditis elegans Collagen alpha-2(IV) chain (let-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OCa349690	NM_078262.5 Latest version!	Caenorhabditis elegans Collagen alpha-2(IV) chain (let-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OCa349690	NM_078262.4	Caenorhabditis elegans Collagen alpha-2(IV) chain (let-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCa349689	NM_078263.3	Caenorhabditis elegans Collagen alpha-2(IV) chain (let-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

let-2 ( NM_078263.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_510664.1 Caenorhabditis elegans Collagen alpha-2(IV) chain (let-2), mRNA.

Price & Availability↑

CloneID	OCa349689
Clone ID Related Accession (Same CDS sequence)	NM_078263.4 , NM_078263.3
Accession Version	NM_078263.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Collagen alpha-2(IV) chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Sep 4, 2019 this sequence version replaced NM_078263.3.

ATGAAGCAAC GAGCGGCTCT CGGGCCCGTG TTGCGACTGG CCATATTGGC CTTACTAGCT 
GTGAGCTATG TTCAATCGCA AGCTACATGC AGAGATTGCT CTAACCGAGG ATGTTTCTGC 
GTCGGAGAAA AAGGATCTAT GGGAGCACCA GGACCACAAG GTCCACCTGG AACCCAAGGA 
ATCCGTGGAT TCCCAGGACC AGAAGGACTC GCTGGACCAA AAGGACTGAA AGGAGCTCAA 
GGACCCCCTG GACCAGTTGG AATCAAGGGA GATCGTGGAG CCGTTGGAGT ACCTGGATTC 
CCAGGAAATG ACGGTGGAAA CGGACGACCA GGAGAGCCAG GACCACCAGG AGCCCCAGGA 
TGGGATGGTT GCAACGGAAC TGACGGAGCT CCAGGAATCC CAGGACGGCC AGGACCACCA 
GGAATGCCAG GATTCCCCGG ACCACCAGGA ATGGACGGTT TGAAGGGAGA GCCAGCTATC 
GGATATGCTG GTGCTCCAGG AGAGAAGGGA GATGGCGGTA TGCCAGGAAT GCCCGGACTT 
CCAGGACCAT CCGGTCGTGA TGGATACCCA GGAGAAAAGG GAGACCGAGG AGATACTGGA 
AATGCTGGAC CACGTGGACC ACCTGGAGAG GCTGGATCCC CAGGAAACCC AGGAATCGGA 
AGCATTGGAC CAAAAGGAGA TCCTGGAGAT CTAGGTTCTG TCGGACCACC AGGTCCACCG 
GGACCACGTG AGTTCACCGG ATCCGGCTCA ATTGTCGGAC CTCGCGGAAA CCCTGGAGAA 
AAGGGAGACA AGGGAGAGCC AGGAGAGGGA GGTCAACGCG GTTACCCAGG AAATGGAGGA 
CTCTCAGGAC AGCCAGGACT CCCAGGAATG AAGGGAGAAA AGGGATTGTC TGGACCAGCT 
GGACCAAGAG GAAAGGAAGG TCGCCCAGGA AACGCTGGAC CACCAGGATT CAAGGGAGAT 
CGTGGTCTTG ACGGACTTGG CGGAATCCCA GGACTTCCAG GCCAAAAGGG AGAAGCTGGA 
TACCCAGGAA GAGATGGGCC AAAGGGTAAC TCCGGACCAC CAGGACCACC AGGAGGTGGT 
ACTTTCAATG ACGGAGCTCC AGGACCACCA GGATTGCCAG GACGACCAGG AAACCCAGGA 
CCACCGGGAA CTGATGGCTA CCCAGGAGCC CCAGGGCCAG CTGGACCAAT CGGAAACACA 
GGAGGGCCAG GTCTTCCAGG TTACCCAGGA AATGAAGGAC TCCCAGGACC AAAGGGAGAT 
AAGGGAGATG GCGGTATTCC AGGAGCGCCA GGAGTCTCTG GACCATCTGG AATTCCAGGA 
CTTCCAGGAC CAAAGGGCGA ACCAGGATAT CGTGGAACCC CAGGACAAAG CATCCCAGGA 
CTTCCAGGAA AGGACGGAAA GCCAGGACTC GATGGAGCCC CAGGCCGCAA GGGAGAAAAT 
GGATTGCCAG GAGTTCGTGG ACCACCAGGA GATTCTCTTA ATGGACTTCC AGGAGCACCA 
GGACAACGCG GAGCACCAGG ACCAAACGGA TATGACGGAC GTGACGGAGT TAACGGTCTT 
CCGGGAGCCC CAGGAACAAA GGGAGACAGA GGAGGTACCT GCTCGGCTTG TGCTCCAGGA 
ACCAAGGGAG AAAAGGGATT GCCAGGATAT TCTGGTCAGC CAGGACCACA AGGAGACCGC 
GGTCTCCCAG GTATGCCAGG ACCAGTCGGA GACGCTGGAG ATGATGGTCT TCCGGGACCA 
GCAGGTCGCC CAGGATCTCC AGGACCACCA GGACAAGACG GTTTCCCAGG ACTCCCAGGA 
CAAAAGGGAG AACCAACTCA GCTAACCCTT AGACCAGGAC CACCAGGTTA TCCAGGATTG 
AAGGGAGAAA ACGGATTCCC AGGACAACCA GGAGTTGATG GACTCCCAGG ACCATCAGGG 
CCAGTCGGAC CACCAGGAGC GCCAGGATAC CCAGGAGAGA AGGGAGACGC CGGTCTTCCA 
GGACTTTCAG GAAAGCCAGG ACAGGACGGA CTTCCAGGAC TCCCAGGAAA TAAGGGAGAA 
GCCGGATACG GACAGCCAGG TCAACCAGGA TTCCCAGGAG CTAAAGGAGA TGGCGGTCTT 
CCAGGACTGC CAGGAACTCC AGGTCTCCAA GGAATGCCAG GAGAGCCAGC TCCAGAAAAC 
CAAGTGAACC CAGCTCCACC AGGTCAACCA GGACTCCCAG GACTTCCAGG AACCAAGGGA 
GAAGGAGGAT ACCCAGGTAG ACCAGGAGAA GTCGGTCAAC CAGGATTCCC AGGACTGCCA 
GGAATGAAGG GAGACTCTGG CCTCCCAGGA CCACCAGGAC TCCCAGGACA CCCAGGTGTT 
CCAGGAGACA AGGGATTCGG AGGTGTCCCA GGATTACCAG GAATTCCAGG ACCAAAGGGA 
GATGTCGGAA ACCCAGGACT CCCAGGTCTT AACGGACAAA AGGGAGAACC AGGAGTTGGA 
GTCCCAGGAC AACCAGGATC ACCAGGATTC CCAGGACTTA AGGGAGACGC CGGTCTTCCA 
GGTCTTCCAG GAACCCCAGG ACTCGAGGGT CAACGTGGAT TCCCAGGAGC CCCAGGACTC 
AAGGGAGGAG ACGGTCTTCC AGGACTGTCT GGTCAGCCAG GATACCCAGG AGAAAAGGGA 
GACGCCGGTT TGCCAGGAGT ACCAGGGCGT GAAGGATCGC CAGGATTCCC AGGTCAAGAT 
GGACTTCCAG GAGTGCCAGG AATGAAGGGA GAAGACGGTC TTCCAGGACT CCCAGGAGTT 
ACTGGACTCA AGGGAGATCT CGGAGCACCA GGACAATCCG GAGCACCAGG ACTCCCAGGA 
GCACCAGGAT ACCCAGGAAT GAAGGGAAAT GCGGGTATCC CAGGAGTGCC AGGATTCAAG 
GGAGACGGAG GACTCCCAGG ACTGCCAGGA CTTAACGGAC CAAAGGGAGA ACCAGGAGTA 
CCAGGAATGC CAGGAACCCC AGGAATGAAG GGTAACGGTG GTCTGCCAGG ATTGCCAGGA 
CGTGATGGAC TTTCCGGAGT ACCAGGAATG AAGGGAGATC GTGGTTTCAA CGGACTTCCA 
GGAGAGAAGG GAGAAGCTGG ACCAGCTGCT CGTGACGGAC AAAAGGGAGA TGCCGGTCTT 
CCAGGACAGC CAGGTCTTCG TGGACCACAA GGACCATCTG GACTTCCAGG TGTCCCAGGA 
TTCAAGGGAG AGACTGGACT TCCAGGATAT GGACAACCAG GTCAACCAGG AGAGAAGGGA 
CTTCCTGGCA TTCCAGGAAA GGCTGGACGT CAAGGAGCCC CAGGGTCACC AGGACAAGAC 
GGACTCCCAG GATTCCCAGG AATGAAGGGA GAATCTGGAT ACCCAGGACA AGACGGCCTT 
CCAGGGCGTG ATGGCCTCCC AGGAGTCCCA GGACAAAAGG GAGATCTTGG ACAGTCTGGA 
CAGCCAGGAC TCTCCGGGGC CCCAGGACTC GACGGTCAAC CAGGAGTACC AGGAATCCGT 
GGAGACAAGG GACAAGGAGG ACTACCCGGA ATCCCAGGAG ATCGTGGAAT GGATGGTTAT 
CCAGGACAAA AGGGAGAGAA CGGTTACCCA GGTCAGCCAG GTCTTCCAGG ACTTGGTGGA 
GAGAAAGGAT TCGCTGGAAC CCCAGGTTTC CCAGGACTTA AGGGATCCCC AGGATACCCA 
GGACAAGACG GACTTCCAGG AATCCCTGGA TTGAAGGGAG ACTCAGGATT CCCAGGACAG 
CCAGGACAAG AAGGACTCCC AGGACTTTCC GGAGAGAAGG GAATGGGTGG ACTCCCAGGA 
ATGCCAGGAC AACCAGGTCA ATCTATTGCT GGACCAGTTG GACCACCAGG TGCCCCAGGA 
CTTCAAGGAA AAGACGGCTT CCCAGGCCTT CCAGGACAAA AGGGAGAATC CGGTCTTTCA 
GGCCTTCCAG GAGCCCCTGG ATTGAAGGGA GAATCCGGCA TGCCAGGATT CCCAGGAGCT 
AAGGGAGATC TAGGAGCCAA TGGAATTCCA GGAAAGCGCG GAGAAGACGG TCTACCAGGA 
GTACCAGGAA GAGATGGACA ACCAGGAATC CCAGGATTGA AGGGAGAAGT TGGAGGTGCT 
GGTCTTCCAG GACAACCAGG CTTCCCAGGT ATCCCAGGAC TTAAGGGAGA AGGAGGCCTT 
CCAGGTTTCC CAGGAGCAAA GGGAGAAGCT GGATTCCCAG GTACCCCAGG AGTGCCAGGA 
TACGCCGGAG AGAAGGGAGA CGGAGGTCTT CCAGGTCTTC CAGGAAGAGA CGGACTCCCA 
GGAGCTGATG GACCAGTGGG ACCACCAGGA CCATCAGGAC CACAAAACCT CGTTGAGCCG 
GGAGAGAAGG GACTTCCAGG TCTTCCAGGA GCCCCAGGAC TTCGTGGAGA GAAGGGAATG 
CCAGGGCTCG ATGGACCACC AGGAAACGAT GGACCACCAG GTCTTCCAGG ACAACGTGGA 
AACGACGGAT ACCCAGGAGC CCCAGGATTA TCAGGAGAGA AGGGAATGGG AGGTCTTCCA 
GGTTTCCCAG GACTTGACGG ACAACCAGGA GGCCCAGGAG CCCCAGGATT GCCAGGAGCA 
CCAGGAGCTG CAGGACCAGC TTACAGAGAC GGATTCGTTC TCGTCAAGCA CTCCCAAACC 
ACCGAAGTAC CAAGATGCCC AGAGGGTCAG ACCAAGCTGT GGGACGGATA TTCCTTGTTG 
TACATTGAAG GAAACGAGAA ATCCCATAAC CAAGACCTCG GACACGCTGG ATCATGCCTC 
CAAAGATTCT CCACCATGCC ATTCCTGTTC TGCGACTTCA ACAACGTCTG CAACTACGCC 
TCCAGAAACG ACAAGTCTTA CTGGCTCTCA ACATCTGAAG CTATCCCAAT GATGCCAGTC 
AACGAGCGTG AAATCGAGCC ATACATCTCG CGCTGCGCTG TGTGCGAAGC CCCAGCCAAC 
ACGATCGCTG TTCACTCCCA GACCATCCAA ATTCCAAACT GCCCAGCAGG ATGGAGCAGC 
TTGTGGATCG GATACTCGTT CGCCATGCAC ACCGGAGCCG GAGCCGAGGG AGGTGGTCAA 
TCACTCTCCT CACCAGGATC CTGCTTGGAA GACTTCCGTG CCACTCCATT CATCGAGTGC 
AACGGTGCCC GCGGATCTTG CCACTACTTT GCCAACAAGT TCTCATTCTG GCTTACCACC 
ATCGACAATG ACTCAGAATT CAAGGTGCCA GAAAGTCAGA CACTCAAGTC AGGAAATCTT 
CGTACCCGCG TCTCAAGATG CCAAGTTTGC GTCAAGTCCA CCGACGGACG TCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_510664.1
CDS	45..5321
Translation	MKQRAALGPVLRLAILALLAVSYVQSQATCRDCSNRGCFCVGEKGSMGAPGPQGPPGTQGIRGFPGPEGLAGPKGLKGAQGPPGPVGIKGDRGAVGVPGFPGNDGGNGRPGEPGPPGAPGWDGCNGTDGAPGIPGRPGPPGMPGFPGPPGMDGLKGEPAIGYAGAPGEKGDGGMPGMPGLPGPSGRDGYPGEKGDRGDTGNAGPRGPPGEAGSPGNPGIGSIGPKGDPGDLGSVGPPGPPGPREFTGSGSIVGPRGNPGEKGDKGEPGEGGQRGYPGNGGLSGQPGLPGMKGEKGLSGPAGPRGKEGRPGNAGPPGFKGDRGLDGLGGIPGLPGQKGEAGYPGRDGPKGNSGPPGPPGGGTFNDGAPGPPGLPGRPGNPGPPGTDGYPGAPGPAGPIGNTGGPGLPGYPGNEGLPGPKGDKGDGGIPGAPGVSGPSGIPGLPGPKGEPGYRGTPGQSIPGLPGKDGKPGLDGAPGRKGENGLPGVRGPPGDSLNGLPGAPGQRGAPGPNGYDGRDGVNGLPGAPGTKGDRGGTCSACAPGTKGEKGLPGYSGQPGPQGDRGLPGMPGPVGDAGDDGLPGPAGRPGSPGPPGQDGFPGLPGQKGEPTQLTLRPGPPGYPGLKGENGFPGQPGVDGLPGPSGPVGPPGAPGYPGEKGDAGLPGLSGKPGQDGLPGLPGNKGEAGYGQPGQPGFPGAKGDGGLPGLPGTPGLQGMPGEPAPENQVNPAPPGQPGLPGLPGTKGEGGYPGRPGEVGQPGFPGLPGMKGDSGLPGPPGLPGHPGVPGDKGFGGVPGLPGIPGPKGDVGNPGLPGLNGQKGEPGVGVPGQPGSPGFPGLKGDAGLPGLPGTPGLEGQRGFPGAPGLKGGDGLPGLSGQPGYPGEKGDAGLPGVPGREGSPGFPGQDGLPGVPGMKGEDGLPGLPGVTGLKGDLGAPGQSGAPGLPGAPGYPGMKGNAGIPGVPGFKGDGGLPGLPGLNGPKGEPGVPGMPGTPGMKGNGGLPGLPGRDGLSGVPGMKGDRGFNGLPGEKGEAGPAARDGQKGDAGLPGQPGLRGPQGPSGLPGVPGFKGETGLPGYGQPGQPGEKGLPGIPGKAGRQGAPGSPGQDGLPGFPGMKGESGYPGQDGLPGRDGLPGVPGQKGDLGQSGQPGLSGAPGLDGQPGVPGIRGDKGQGGLPGIPGDRGMDGYPGQKGENGYPGQPGLPGLGGEKGFAGTPGFPGLKGSPGYPGQDGLPGIPGLKGDSGFPGQPGQEGLPGLSGEKGMGGLPGMPGQPGQSIAGPVGPPGAPGLQGKDGFPGLPGQKGESGLSGLPGAPGLKGESGMPGFPGAKGDLGANGIPGKRGEDGLPGVPGRDGQPGIPGLKGEVGGAGLPGQPGFPGIPGLKGEGGLPGFPGAKGEAGFPGTPGVPGYAGEKGDGGLPGLPGRDGLPGADGPVGPPGPSGPQNLVEPGEKGLPGLPGAPGLRGEKGMPGLDGPPGNDGPPGLPGQRGNDGYPGAPGLSGEKGMGGLPGFPGLDGQPGGPGAPGLPGAPGAAGPAYRDGFVLVKHSQTTEVPRCPEGQTKLWDGYSLLYIEGNEKSHNQDLGHAGSCLQRFSTMPFLFCDFNNVCNYASRNDKSYWLSTSEAIPMMPVNEREIEPYISRCAVCEAPANTIAVHSQTIQIPNCPAGWSSLWIGYSFAMHTGAGAEGGGQSLSSPGSCLEDFRATPFIECNGARGSCHYFANKFSFWLTTIDNDSEFKVPESQTLKSGNLRTRVSRCQVCVKSTDGRH

Target ORF information:

RefSeq Version	NM_078263.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Collagen alpha-2(IV) chain (let-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078263.4

ORF Insert Sequence:

ATGAAGCAAC GAGCGGCTCT CGGGCCCGTG TTGCGACTGG CCATATTGGC CTTACTAGCT 
GTGAGCTATG TTCAATCGCA AGCTACATGC AGAGATTGCT CTAACCGAGG ATGTTTCTGC 
GTCGGAGAAA AAGGATCTAT GGGAGCACCA GGACCACAAG GTCCACCTGG AACCCAAGGA 
ATCCGTGGAT TCCCAGGACC AGAAGGACTC GCTGGACCAA AAGGACTGAA AGGAGCTCAA 
GGACCCCCTG GACCAGTTGG AATCAAGGGA GATCGTGGAG CCGTTGGAGT ACCTGGATTC 
CCAGGAAATG ACGGTGGAAA CGGACGACCA GGAGAGCCAG GACCACCAGG AGCCCCAGGA 
TGGGATGGTT GCAACGGAAC TGACGGAGCT CCAGGAATCC CAGGACGGCC AGGACCACCA 
GGAATGCCAG GATTCCCCGG ACCACCAGGA ATGGACGGTT TGAAGGGAGA GCCAGCTATC 
GGATATGCTG GTGCTCCAGG AGAGAAGGGA GATGGCGGTA TGCCAGGAAT GCCCGGACTT 
CCAGGACCAT CCGGTCGTGA TGGATACCCA GGAGAAAAGG GAGACCGAGG AGATACTGGA 
AATGCTGGAC CACGTGGACC ACCTGGAGAG GCTGGATCCC CAGGAAACCC AGGAATCGGA 
AGCATTGGAC CAAAAGGAGA TCCTGGAGAT CTAGGTTCTG TCGGACCACC AGGTCCACCG 
GGACCACGTG AGTTCACCGG ATCCGGCTCA ATTGTCGGAC CTCGCGGAAA CCCTGGAGAA 
AAGGGAGACA AGGGAGAGCC AGGAGAGGGA GGTCAACGCG GTTACCCAGG AAATGGAGGA 
CTCTCAGGAC AGCCAGGACT CCCAGGAATG AAGGGAGAAA AGGGATTGTC TGGACCAGCT 
GGACCAAGAG GAAAGGAAGG TCGCCCAGGA AACGCTGGAC CACCAGGATT CAAGGGAGAT 
CGTGGTCTTG ACGGACTTGG CGGAATCCCA GGACTTCCAG GCCAAAAGGG AGAAGCTGGA 
TACCCAGGAA GAGATGGGCC AAAGGGTAAC TCCGGACCAC CAGGACCACC AGGAGGTGGT 
ACTTTCAATG ACGGAGCTCC AGGACCACCA GGATTGCCAG GACGACCAGG AAACCCAGGA 
CCACCGGGAA CTGATGGCTA CCCAGGAGCC CCAGGGCCAG CTGGACCAAT CGGAAACACA 
GGAGGGCCAG GTCTTCCAGG TTACCCAGGA AATGAAGGAC TCCCAGGACC AAAGGGAGAT 
AAGGGAGATG GCGGTATTCC AGGAGCGCCA GGAGTCTCTG GACCATCTGG AATTCCAGGA 
CTTCCAGGAC CAAAGGGCGA ACCAGGATAT CGTGGAACCC CAGGACAAAG CATCCCAGGA 
CTTCCAGGAA AGGACGGAAA GCCAGGACTC GATGGAGCCC CAGGCCGCAA GGGAGAAAAT 
GGATTGCCAG GAGTTCGTGG ACCACCAGGA GATTCTCTTA ATGGACTTCC AGGAGCACCA 
GGACAACGCG GAGCACCAGG ACCAAACGGA TATGACGGAC GTGACGGAGT TAACGGTCTT 
CCGGGAGCCC CAGGAACAAA GGGAGACAGA GGAGGTACCT GCTCGGCTTG TGCTCCAGGA 
ACCAAGGGAG AAAAGGGATT GCCAGGATAT TCTGGTCAGC CAGGACCACA AGGAGACCGC 
GGTCTCCCAG GTATGCCAGG ACCAGTCGGA GACGCTGGAG ATGATGGTCT TCCGGGACCA 
GCAGGTCGCC CAGGATCTCC AGGACCACCA GGACAAGACG GTTTCCCAGG ACTCCCAGGA 
CAAAAGGGAG AACCAACTCA GCTAACCCTT AGACCAGGAC CACCAGGTTA TCCAGGATTG 
AAGGGAGAAA ACGGATTCCC AGGACAACCA GGAGTTGATG GACTCCCAGG ACCATCAGGG 
CCAGTCGGAC CACCAGGAGC GCCAGGATAC CCAGGAGAGA AGGGAGACGC CGGTCTTCCA 
GGACTTTCAG GAAAGCCAGG ACAGGACGGA CTTCCAGGAC TCCCAGGAAA TAAGGGAGAA 
GCCGGATACG GACAGCCAGG TCAACCAGGA TTCCCAGGAG CTAAAGGAGA TGGCGGTCTT 
CCAGGACTGC CAGGAACTCC AGGTCTCCAA GGAATGCCAG GAGAGCCAGC TCCAGAAAAC 
CAAGTGAACC CAGCTCCACC AGGTCAACCA GGACTCCCAG GACTTCCAGG AACCAAGGGA 
GAAGGAGGAT ACCCAGGTAG ACCAGGAGAA GTCGGTCAAC CAGGATTCCC AGGACTGCCA 
GGAATGAAGG GAGACTCTGG CCTCCCAGGA CCACCAGGAC TCCCAGGACA CCCAGGTGTT 
CCAGGAGACA AGGGATTCGG AGGTGTCCCA GGATTACCAG GAATTCCAGG ACCAAAGGGA 
GATGTCGGAA ACCCAGGACT CCCAGGTCTT AACGGACAAA AGGGAGAACC AGGAGTTGGA 
GTCCCAGGAC AACCAGGATC ACCAGGATTC CCAGGACTTA AGGGAGACGC CGGTCTTCCA 
GGTCTTCCAG GAACCCCAGG ACTCGAGGGT CAACGTGGAT TCCCAGGAGC CCCAGGACTC 
AAGGGAGGAG ACGGTCTTCC AGGACTGTCT GGTCAGCCAG GATACCCAGG AGAAAAGGGA 
GACGCCGGTT TGCCAGGAGT ACCAGGGCGT GAAGGATCGC CAGGATTCCC AGGTCAAGAT 
GGACTTCCAG GAGTGCCAGG AATGAAGGGA GAAGACGGTC TTCCAGGACT CCCAGGAGTT 
ACTGGACTCA AGGGAGATCT CGGAGCACCA GGACAATCCG GAGCACCAGG ACTCCCAGGA 
GCACCAGGAT ACCCAGGAAT GAAGGGAAAT GCGGGTATCC CAGGAGTGCC AGGATTCAAG 
GGAGACGGAG GACTCCCAGG ACTGCCAGGA CTTAACGGAC CAAAGGGAGA ACCAGGAGTA 
CCAGGAATGC CAGGAACCCC AGGAATGAAG GGTAACGGTG GTCTGCCAGG ATTGCCAGGA 
CGTGATGGAC TTTCCGGAGT ACCAGGAATG AAGGGAGATC GTGGTTTCAA CGGACTTCCA 
GGAGAGAAGG GAGAAGCTGG ACCAGCTGCT CGTGACGGAC AAAAGGGAGA TGCCGGTCTT 
CCAGGACAGC CAGGTCTTCG TGGACCACAA GGACCATCTG GACTTCCAGG TGTCCCAGGA 
TTCAAGGGAG AGACTGGACT TCCAGGATAT GGACAACCAG GTCAACCAGG AGAGAAGGGA 
CTTCCTGGCA TTCCAGGAAA GGCTGGACGT CAAGGAGCCC CAGGGTCACC AGGACAAGAC 
GGACTCCCAG GATTCCCAGG AATGAAGGGA GAATCTGGAT ACCCAGGACA AGACGGCCTT 
CCAGGGCGTG ATGGCCTCCC AGGAGTCCCA GGACAAAAGG GAGATCTTGG ACAGTCTGGA 
CAGCCAGGAC TCTCCGGGGC CCCAGGACTC GACGGTCAAC CAGGAGTACC AGGAATCCGT 
GGAGACAAGG GACAAGGAGG ACTACCCGGA ATCCCAGGAG ATCGTGGAAT GGATGGTTAT 
CCAGGACAAA AGGGAGAGAA CGGTTACCCA GGTCAGCCAG GTCTTCCAGG ACTTGGTGGA 
GAGAAAGGAT TCGCTGGAAC CCCAGGTTTC CCAGGACTTA AGGGATCCCC AGGATACCCA 
GGACAAGACG GACTTCCAGG AATCCCTGGA TTGAAGGGAG ACTCAGGATT CCCAGGACAG 
CCAGGACAAG AAGGACTCCC AGGACTTTCC GGAGAGAAGG GAATGGGTGG ACTCCCAGGA 
ATGCCAGGAC AACCAGGTCA ATCTATTGCT GGACCAGTTG GACCACCAGG TGCCCCAGGA 
CTTCAAGGAA AAGACGGCTT CCCAGGCCTT CCAGGACAAA AGGGAGAATC CGGTCTTTCA 
GGCCTTCCAG GAGCCCCTGG ATTGAAGGGA GAATCCGGCA TGCCAGGATT CCCAGGAGCT 
AAGGGAGATC TAGGAGCCAA TGGAATTCCA GGAAAGCGCG GAGAAGACGG TCTACCAGGA 
GTACCAGGAA GAGATGGACA ACCAGGAATC CCAGGATTGA AGGGAGAAGT TGGAGGTGCT 
GGTCTTCCAG GACAACCAGG CTTCCCAGGT ATCCCAGGAC TTAAGGGAGA AGGAGGCCTT 
CCAGGTTTCC CAGGAGCAAA GGGAGAAGCT GGATTCCCAG GTACCCCAGG AGTGCCAGGA 
TACGCCGGAG AGAAGGGAGA CGGAGGTCTT CCAGGTCTTC CAGGAAGAGA CGGACTCCCA 
GGAGCTGATG GACCAGTGGG ACCACCAGGA CCATCAGGAC CACAAAACCT CGTTGAGCCG 
GGAGAGAAGG GACTTCCAGG TCTTCCAGGA GCCCCAGGAC TTCGTGGAGA GAAGGGAATG 
CCAGGGCTCG ATGGACCACC AGGAAACGAT GGACCACCAG GTCTTCCAGG ACAACGTGGA 
AACGACGGAT ACCCAGGAGC CCCAGGATTA TCAGGAGAGA AGGGAATGGG AGGTCTTCCA 
GGTTTCCCAG GACTTGACGG ACAACCAGGA GGCCCAGGAG CCCCAGGATT GCCAGGAGCA 
CCAGGAGCTG CAGGACCAGC TTACAGAGAC GGATTCGTTC TCGTCAAGCA CTCCCAAACC 
ACCGAAGTAC CAAGATGCCC AGAGGGTCAG ACCAAGCTGT GGGACGGATA TTCCTTGTTG 
TACATTGAAG GAAACGAGAA ATCCCATAAC CAAGACCTCG GACACGCTGG ATCATGCCTC 
CAAAGATTCT CCACCATGCC ATTCCTGTTC TGCGACTTCA ACAACGTCTG CAACTACGCC 
TCCAGAAACG ACAAGTCTTA CTGGCTCTCA ACATCTGAAG CTATCCCAAT GATGCCAGTC 
AACGAGCGTG AAATCGAGCC ATACATCTCG CGCTGCGCTG TGTGCGAAGC CCCAGCCAAC 
ACGATCGCTG TTCACTCCCA GACCATCCAA ATTCCAAACT GCCCAGCAGG ATGGAGCAGC 
TTGTGGATCG GATACTCGTT CGCCATGCAC ACCGGAGCCG GAGCCGAGGG AGGTGGTCAA 
TCACTCTCCT CACCAGGATC CTGCTTGGAA GACTTCCGTG CCACTCCATT CATCGAGTGC 
AACGGTGCCC GCGGATCTTG CCACTACTTT GCCAACAAGT TCTCATTCTG GCTTACCACC 
ATCGACAATG ACTCAGAATT CAAGGTGCCA GAAAGTCAGA CACTCAAGTC AGGAAATCTT 
CGTACCCGCG TCTCAAGATG CCAAGTTTGC GTCAAGTCCA CCGACGGACG TCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

let-2 ( NM_078262.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_510663.1 Caenorhabditis elegans Collagen alpha-2(IV) chain (let-2), mRNA.

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CloneID	OCa349690
Clone ID Related Accession (Same CDS sequence)	NM_078262.4 , NM_078262.5
Accession Version	NM_078262.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Collagen alpha-2(IV) chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Sep 4, 2019 this sequence version replaced NM_078262.4.

ATGAAGCAAC GAGCGGCTCT CGGGCCCGTG TTGCGACTGG CCATATTGGC CTTACTAGCT 
GTGAGCTATG TTCAATCGCA AGCTACATGC AGAGATTGCT CTAACCGAGG ATGTTTCTGC 
GTCGGAGAAA AAGGATCTAT GGGAGCACCA GGACCACAAG GTCCACCTGG AACCCAAGGA 
ATCCGTGGAT TCCCAGGACC AGAAGGACTC GCTGGACCAA AAGGACTGAA AGGAGCTCAA 
GGACCCCCTG GACCAGTTGG AATCAAGGGA GATCGTGGAG CCGTTGGAGT ACCTGGATTC 
CCAGGAAATG ACGGTGGAAA CGGACGACCA GGAGAGCCAG GACCACCAGG AGCCCCAGGA 
TGGGATGGTT GCAACGGAAC TGACGGAGCT CCAGGAATCC CAGGACGGCC AGGACCACCA 
GGAATGCCAG GATTCCCCGG ACCACCAGGA ATGGACGGTT TGAAGGGAGA GCCAGCTATC 
GGATATGCTG GTGCTCCAGG AGAGAAGGGA GATGGCGGTA TGCCAGGAAT GCCCGGACTT 
CCAGGACCAT CCGGTCGTGA TGGATACCCA GGAGAAAAGG GAGACCGAGG AGATACTGGA 
AATGCTGGAC CACGTGGACC ACCTGGAGAG GCTGGATCCC CAGGAAACCC AGGAATCGGA 
AGCATTGGAC CAAAAGGAGA TCCTGGAGAC ATTGGTGCGA TGGGACCGGC CGGTCCGCCA 
GGCCCAATCG CCTCCACCAT GTCCAAGGGA ACCATTATCG GTCCTAAGGG AGACCTAGGA 
GAGAAGGGAG AGAAGGGAGA GCCAGGAGAG GGAGGTCAAC GCGGTTACCC AGGAAATGGA 
GGACTCTCAG GACAGCCAGG ACTCCCAGGA ATGAAGGGAG AAAAGGGATT GTCTGGACCA 
GCTGGACCAA GAGGAAAGGA AGGTCGCCCA GGAAACGCTG GACCACCAGG ATTCAAGGGA 
GATCGTGGTC TTGACGGACT TGGCGGAATC CCAGGACTTC CAGGCCAAAA GGGAGAAGCT 
GGATACCCAG GAAGAGATGG GCCAAAGGGT AACTCCGGAC CACCAGGACC ACCAGGAGGT 
GGTACTTTCA ATGACGGAGC TCCAGGACCA CCAGGATTGC CAGGACGACC AGGAAACCCA 
GGACCACCGG GAACTGATGG CTACCCAGGA GCCCCAGGGC CAGCTGGACC AATCGGAAAC 
ACAGGAGGGC CAGGTCTTCC AGGTTACCCA GGAAATGAAG GACTCCCAGG ACCAAAGGGA 
GATAAGGGAG ATGGCGGTAT TCCAGGAGCG CCAGGAGTCT CTGGACCATC TGGAATTCCA 
GGACTTCCAG GACCAAAGGG CGAACCAGGA TATCGTGGAA CCCCAGGACA AAGCATCCCA 
GGACTTCCAG GAAAGGACGG AAAGCCAGGA CTCGATGGAG CCCCAGGCCG CAAGGGAGAA 
AATGGATTGC CAGGAGTTCG TGGACCACCA GGAGATTCTC TTAATGGACT TCCAGGAGCA 
CCAGGACAAC GCGGAGCACC AGGACCAAAC GGATATGACG GACGTGACGG AGTTAACGGT 
CTTCCGGGAG CCCCAGGAAC AAAGGGAGAC AGAGGAGGTA CCTGCTCGGC TTGTGCTCCA 
GGAACCAAGG GAGAAAAGGG ATTGCCAGGA TATTCTGGTC AGCCAGGACC ACAAGGAGAC 
CGCGGTCTCC CAGGTATGCC AGGACCAGTC GGAGACGCTG GAGATGATGG TCTTCCGGGA 
CCAGCAGGTC GCCCAGGATC TCCAGGACCA CCAGGACAAG ACGGTTTCCC AGGACTCCCA 
GGACAAAAGG GAGAACCAAC TCAGCTAACC CTTAGACCAG GACCACCAGG TTATCCAGGA 
TTGAAGGGAG AAAACGGATT CCCAGGACAA CCAGGAGTTG ATGGACTCCC AGGACCATCA 
GGGCCAGTCG GACCACCAGG AGCGCCAGGA TACCCAGGAG AGAAGGGAGA CGCCGGTCTT 
CCAGGACTTT CAGGAAAGCC AGGACAGGAC GGACTTCCAG GACTCCCAGG AAATAAGGGA 
GAAGCCGGAT ACGGACAGCC AGGTCAACCA GGATTCCCAG GAGCTAAAGG AGATGGCGGT 
CTTCCAGGAC TGCCAGGAAC TCCAGGTCTC CAAGGAATGC CAGGAGAGCC AGCTCCAGAA 
AACCAAGTGA ACCCAGCTCC ACCAGGTCAA CCAGGACTCC CAGGACTTCC AGGAACCAAG 
GGAGAAGGAG GATACCCAGG TAGACCAGGA GAAGTCGGTC AACCAGGATT CCCAGGACTG 
CCAGGAATGA AGGGAGACTC TGGCCTCCCA GGACCACCAG GACTCCCAGG ACACCCAGGT 
GTTCCAGGAG ACAAGGGATT CGGAGGTGTC CCAGGATTAC CAGGAATTCC AGGACCAAAG 
GGAGATGTCG GAAACCCAGG ACTCCCAGGT CTTAACGGAC AAAAGGGAGA ACCAGGAGTT 
GGAGTCCCAG GACAACCAGG ATCACCAGGA TTCCCAGGAC TTAAGGGAGA CGCCGGTCTT 
CCAGGTCTTC CAGGAACCCC AGGACTCGAG GGTCAACGTG GATTCCCAGG AGCCCCAGGA 
CTCAAGGGAG GAGACGGTCT TCCAGGACTG TCTGGTCAGC CAGGATACCC AGGAGAAAAG 
GGAGACGCCG GTTTGCCAGG AGTACCAGGG CGTGAAGGAT CGCCAGGATT CCCAGGTCAA 
GATGGACTTC CAGGAGTGCC AGGAATGAAG GGAGAAGACG GTCTTCCAGG ACTCCCAGGA 
GTTACTGGAC TCAAGGGAGA TCTCGGAGCA CCAGGACAAT CCGGAGCACC AGGACTCCCA 
GGAGCACCAG GATACCCAGG AATGAAGGGA AATGCGGGTA TCCCAGGAGT GCCAGGATTC 
AAGGGAGACG GAGGACTCCC AGGACTGCCA GGACTTAACG GACCAAAGGG AGAACCAGGA 
GTACCAGGAA TGCCAGGAAC CCCAGGAATG AAGGGTAACG GTGGTCTGCC AGGATTGCCA 
GGACGTGATG GACTTTCCGG AGTACCAGGA ATGAAGGGAG ATCGTGGTTT CAACGGACTT 
CCAGGAGAGA AGGGAGAAGC TGGACCAGCT GCTCGTGACG GACAAAAGGG AGATGCCGGT 
CTTCCAGGAC AGCCAGGTCT TCGTGGACCA CAAGGACCAT CTGGACTTCC AGGTGTCCCA 
GGATTCAAGG GAGAGACTGG ACTTCCAGGA TATGGACAAC CAGGTCAACC AGGAGAGAAG 
GGACTTCCTG GCATTCCAGG AAAGGCTGGA CGTCAAGGAG CCCCAGGGTC ACCAGGACAA 
GACGGACTCC CAGGATTCCC AGGAATGAAG GGAGAATCTG GATACCCAGG ACAAGACGGC 
CTTCCAGGGC GTGATGGCCT CCCAGGAGTC CCAGGACAAA AGGGAGATCT TGGACAGTCT 
GGACAGCCAG GACTCTCCGG GGCCCCAGGA CTCGACGGTC AACCAGGAGT ACCAGGAATC 
CGTGGAGACA AGGGACAAGG AGGACTACCC GGAATCCCAG GAGATCGTGG AATGGATGGT 
TATCCAGGAC AAAAGGGAGA GAACGGTTAC CCAGGTCAGC CAGGTCTTCC AGGACTTGGT 
GGAGAGAAAG GATTCGCTGG AACCCCAGGT TTCCCAGGAC TTAAGGGATC CCCAGGATAC 
CCAGGACAAG ACGGACTTCC AGGAATCCCT GGATTGAAGG GAGACTCAGG ATTCCCAGGA 
CAGCCAGGAC AAGAAGGACT CCCAGGACTT TCCGGAGAGA AGGGAATGGG TGGACTCCCA 
GGAATGCCAG GACAACCAGG TCAATCTATT GCTGGACCAG TTGGACCACC AGGTGCCCCA 
GGACTTCAAG GAAAAGACGG CTTCCCAGGC CTTCCAGGAC AAAAGGGAGA ATCCGGTCTT 
TCAGGCCTTC CAGGAGCCCC TGGATTGAAG GGAGAATCCG GCATGCCAGG ATTCCCAGGA 
GCTAAGGGAG ATCTAGGAGC CAATGGAATT CCAGGAAAGC GCGGAGAAGA CGGTCTACCA 
GGAGTACCAG GAAGAGATGG ACAACCAGGA ATCCCAGGAT TGAAGGGAGA AGTTGGAGGT 
GCTGGTCTTC CAGGACAACC AGGCTTCCCA GGTATCCCAG GACTTAAGGG AGAAGGAGGC 
CTTCCAGGTT TCCCAGGAGC AAAGGGAGAA GCTGGATTCC CAGGTACCCC AGGAGTGCCA 
GGATACGCCG GAGAGAAGGG AGACGGAGGT CTTCCAGGTC TTCCAGGAAG AGACGGACTC 
CCAGGAGCTG ATGGACCAGT GGGACCACCA GGACCATCAG GACCACAAAA CCTCGTTGAG 
CCGGGAGAGA AGGGACTTCC AGGTCTTCCA GGAGCCCCAG GACTTCGTGG AGAGAAGGGA 
ATGCCAGGGC TCGATGGACC ACCAGGAAAC GATGGACCAC CAGGTCTTCC AGGACAACGT 
GGAAACGACG GATACCCAGG AGCCCCAGGA TTATCAGGAG AGAAGGGAAT GGGAGGTCTT 
CCAGGTTTCC CAGGACTTGA CGGACAACCA GGAGGCCCAG GAGCCCCAGG ATTGCCAGGA 
GCACCAGGAG CTGCAGGACC AGCTTACAGA GACGGATTCG TTCTCGTCAA GCACTCCCAA 
ACCACCGAAG TACCAAGATG CCCAGAGGGT CAGACCAAGC TGTGGGACGG ATATTCCTTG 
TTGTACATTG AAGGAAACGA GAAATCCCAT AACCAAGACC TCGGACACGC TGGATCATGC 
CTCCAAAGAT TCTCCACCAT GCCATTCCTG TTCTGCGACT TCAACAACGT CTGCAACTAC 
GCCTCCAGAA ACGACAAGTC TTACTGGCTC TCAACATCTG AAGCTATCCC AATGATGCCA 
GTCAACGAGC GTGAAATCGA GCCATACATC TCGCGCTGCG CTGTGTGCGA AGCCCCAGCC 
AACACGATCG CTGTTCACTC CCAGACCATC CAAATTCCAA ACTGCCCAGC AGGATGGAGC 
AGCTTGTGGA TCGGATACTC GTTCGCCATG CACACCGGAG CCGGAGCCGA GGGAGGTGGT 
CAATCACTCT CCTCACCAGG ATCCTGCTTG GAAGACTTCC GTGCCACTCC ATTCATCGAG 
TGCAACGGTG CCCGCGGATC TTGCCACTAC TTTGCCAACA AGTTCTCATT CTGGCTTACC 
ACCATCGACA ATGACTCAGA ATTCAAGGTG CCAGAAAGTC AGACACTCAA GTCAGGAAAT 
CTTCGTACCC GCGTCTCAAG ATGCCAAGTT TGCGTCAAGT CCACCGACGG ACGTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_510663.1
CDS	45..5324
Translation	MKQRAALGPVLRLAILALLAVSYVQSQATCRDCSNRGCFCVGEKGSMGAPGPQGPPGTQGIRGFPGPEGLAGPKGLKGAQGPPGPVGIKGDRGAVGVPGFPGNDGGNGRPGEPGPPGAPGWDGCNGTDGAPGIPGRPGPPGMPGFPGPPGMDGLKGEPAIGYAGAPGEKGDGGMPGMPGLPGPSGRDGYPGEKGDRGDTGNAGPRGPPGEAGSPGNPGIGSIGPKGDPGDIGAMGPAGPPGPIASTMSKGTIIGPKGDLGEKGEKGEPGEGGQRGYPGNGGLSGQPGLPGMKGEKGLSGPAGPRGKEGRPGNAGPPGFKGDRGLDGLGGIPGLPGQKGEAGYPGRDGPKGNSGPPGPPGGGTFNDGAPGPPGLPGRPGNPGPPGTDGYPGAPGPAGPIGNTGGPGLPGYPGNEGLPGPKGDKGDGGIPGAPGVSGPSGIPGLPGPKGEPGYRGTPGQSIPGLPGKDGKPGLDGAPGRKGENGLPGVRGPPGDSLNGLPGAPGQRGAPGPNGYDGRDGVNGLPGAPGTKGDRGGTCSACAPGTKGEKGLPGYSGQPGPQGDRGLPGMPGPVGDAGDDGLPGPAGRPGSPGPPGQDGFPGLPGQKGEPTQLTLRPGPPGYPGLKGENGFPGQPGVDGLPGPSGPVGPPGAPGYPGEKGDAGLPGLSGKPGQDGLPGLPGNKGEAGYGQPGQPGFPGAKGDGGLPGLPGTPGLQGMPGEPAPENQVNPAPPGQPGLPGLPGTKGEGGYPGRPGEVGQPGFPGLPGMKGDSGLPGPPGLPGHPGVPGDKGFGGVPGLPGIPGPKGDVGNPGLPGLNGQKGEPGVGVPGQPGSPGFPGLKGDAGLPGLPGTPGLEGQRGFPGAPGLKGGDGLPGLSGQPGYPGEKGDAGLPGVPGREGSPGFPGQDGLPGVPGMKGEDGLPGLPGVTGLKGDLGAPGQSGAPGLPGAPGYPGMKGNAGIPGVPGFKGDGGLPGLPGLNGPKGEPGVPGMPGTPGMKGNGGLPGLPGRDGLSGVPGMKGDRGFNGLPGEKGEAGPAARDGQKGDAGLPGQPGLRGPQGPSGLPGVPGFKGETGLPGYGQPGQPGEKGLPGIPGKAGRQGAPGSPGQDGLPGFPGMKGESGYPGQDGLPGRDGLPGVPGQKGDLGQSGQPGLSGAPGLDGQPGVPGIRGDKGQGGLPGIPGDRGMDGYPGQKGENGYPGQPGLPGLGGEKGFAGTPGFPGLKGSPGYPGQDGLPGIPGLKGDSGFPGQPGQEGLPGLSGEKGMGGLPGMPGQPGQSIAGPVGPPGAPGLQGKDGFPGLPGQKGESGLSGLPGAPGLKGESGMPGFPGAKGDLGANGIPGKRGEDGLPGVPGRDGQPGIPGLKGEVGGAGLPGQPGFPGIPGLKGEGGLPGFPGAKGEAGFPGTPGVPGYAGEKGDGGLPGLPGRDGLPGADGPVGPPGPSGPQNLVEPGEKGLPGLPGAPGLRGEKGMPGLDGPPGNDGPPGLPGQRGNDGYPGAPGLSGEKGMGGLPGFPGLDGQPGGPGAPGLPGAPGAAGPAYRDGFVLVKHSQTTEVPRCPEGQTKLWDGYSLLYIEGNEKSHNQDLGHAGSCLQRFSTMPFLFCDFNNVCNYASRNDKSYWLSTSEAIPMMPVNEREIEPYISRCAVCEAPANTIAVHSQTIQIPNCPAGWSSLWIGYSFAMHTGAGAEGGGQSLSSPGSCLEDFRATPFIECNGARGSCHYFANKFSFWLTTIDNDSEFKVPESQTLKSGNLRTRVSRCQVCVKSTDGRH

Target ORF information:

RefSeq Version	NM_078262.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Collagen alpha-2(IV) chain (let-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078262.5

ORF Insert Sequence:

ATGAAGCAAC GAGCGGCTCT CGGGCCCGTG TTGCGACTGG CCATATTGGC CTTACTAGCT 
GTGAGCTATG TTCAATCGCA AGCTACATGC AGAGATTGCT CTAACCGAGG ATGTTTCTGC 
GTCGGAGAAA AAGGATCTAT GGGAGCACCA GGACCACAAG GTCCACCTGG AACCCAAGGA 
ATCCGTGGAT TCCCAGGACC AGAAGGACTC GCTGGACCAA AAGGACTGAA AGGAGCTCAA 
GGACCCCCTG GACCAGTTGG AATCAAGGGA GATCGTGGAG CCGTTGGAGT ACCTGGATTC 
CCAGGAAATG ACGGTGGAAA CGGACGACCA GGAGAGCCAG GACCACCAGG AGCCCCAGGA 
TGGGATGGTT GCAACGGAAC TGACGGAGCT CCAGGAATCC CAGGACGGCC AGGACCACCA 
GGAATGCCAG GATTCCCCGG ACCACCAGGA ATGGACGGTT TGAAGGGAGA GCCAGCTATC 
GGATATGCTG GTGCTCCAGG AGAGAAGGGA GATGGCGGTA TGCCAGGAAT GCCCGGACTT 
CCAGGACCAT CCGGTCGTGA TGGATACCCA GGAGAAAAGG GAGACCGAGG AGATACTGGA 
AATGCTGGAC CACGTGGACC ACCTGGAGAG GCTGGATCCC CAGGAAACCC AGGAATCGGA 
AGCATTGGAC CAAAAGGAGA TCCTGGAGAC ATTGGTGCGA TGGGACCGGC CGGTCCGCCA 
GGCCCAATCG CCTCCACCAT GTCCAAGGGA ACCATTATCG GTCCTAAGGG AGACCTAGGA 
GAGAAGGGAG AGAAGGGAGA GCCAGGAGAG GGAGGTCAAC GCGGTTACCC AGGAAATGGA 
GGACTCTCAG GACAGCCAGG ACTCCCAGGA ATGAAGGGAG AAAAGGGATT GTCTGGACCA 
GCTGGACCAA GAGGAAAGGA AGGTCGCCCA GGAAACGCTG GACCACCAGG ATTCAAGGGA 
GATCGTGGTC TTGACGGACT TGGCGGAATC CCAGGACTTC CAGGCCAAAA GGGAGAAGCT 
GGATACCCAG GAAGAGATGG GCCAAAGGGT AACTCCGGAC CACCAGGACC ACCAGGAGGT 
GGTACTTTCA ATGACGGAGC TCCAGGACCA CCAGGATTGC CAGGACGACC AGGAAACCCA 
GGACCACCGG GAACTGATGG CTACCCAGGA GCCCCAGGGC CAGCTGGACC AATCGGAAAC 
ACAGGAGGGC CAGGTCTTCC AGGTTACCCA GGAAATGAAG GACTCCCAGG ACCAAAGGGA 
GATAAGGGAG ATGGCGGTAT TCCAGGAGCG CCAGGAGTCT CTGGACCATC TGGAATTCCA 
GGACTTCCAG GACCAAAGGG CGAACCAGGA TATCGTGGAA CCCCAGGACA AAGCATCCCA 
GGACTTCCAG GAAAGGACGG AAAGCCAGGA CTCGATGGAG CCCCAGGCCG CAAGGGAGAA 
AATGGATTGC CAGGAGTTCG TGGACCACCA GGAGATTCTC TTAATGGACT TCCAGGAGCA 
CCAGGACAAC GCGGAGCACC AGGACCAAAC GGATATGACG GACGTGACGG AGTTAACGGT 
CTTCCGGGAG CCCCAGGAAC AAAGGGAGAC AGAGGAGGTA CCTGCTCGGC TTGTGCTCCA 
GGAACCAAGG GAGAAAAGGG ATTGCCAGGA TATTCTGGTC AGCCAGGACC ACAAGGAGAC 
CGCGGTCTCC CAGGTATGCC AGGACCAGTC GGAGACGCTG GAGATGATGG TCTTCCGGGA 
CCAGCAGGTC GCCCAGGATC TCCAGGACCA CCAGGACAAG ACGGTTTCCC AGGACTCCCA 
GGACAAAAGG GAGAACCAAC TCAGCTAACC CTTAGACCAG GACCACCAGG TTATCCAGGA 
TTGAAGGGAG AAAACGGATT CCCAGGACAA CCAGGAGTTG ATGGACTCCC AGGACCATCA 
GGGCCAGTCG GACCACCAGG AGCGCCAGGA TACCCAGGAG AGAAGGGAGA CGCCGGTCTT 
CCAGGACTTT CAGGAAAGCC AGGACAGGAC GGACTTCCAG GACTCCCAGG AAATAAGGGA 
GAAGCCGGAT ACGGACAGCC AGGTCAACCA GGATTCCCAG GAGCTAAAGG AGATGGCGGT 
CTTCCAGGAC TGCCAGGAAC TCCAGGTCTC CAAGGAATGC CAGGAGAGCC AGCTCCAGAA 
AACCAAGTGA ACCCAGCTCC ACCAGGTCAA CCAGGACTCC CAGGACTTCC AGGAACCAAG 
GGAGAAGGAG GATACCCAGG TAGACCAGGA GAAGTCGGTC AACCAGGATT CCCAGGACTG 
CCAGGAATGA AGGGAGACTC TGGCCTCCCA GGACCACCAG GACTCCCAGG ACACCCAGGT 
GTTCCAGGAG ACAAGGGATT CGGAGGTGTC CCAGGATTAC CAGGAATTCC AGGACCAAAG 
GGAGATGTCG GAAACCCAGG ACTCCCAGGT CTTAACGGAC AAAAGGGAGA ACCAGGAGTT 
GGAGTCCCAG GACAACCAGG ATCACCAGGA TTCCCAGGAC TTAAGGGAGA CGCCGGTCTT 
CCAGGTCTTC CAGGAACCCC AGGACTCGAG GGTCAACGTG GATTCCCAGG AGCCCCAGGA 
CTCAAGGGAG GAGACGGTCT TCCAGGACTG TCTGGTCAGC CAGGATACCC AGGAGAAAAG 
GGAGACGCCG GTTTGCCAGG AGTACCAGGG CGTGAAGGAT CGCCAGGATT CCCAGGTCAA 
GATGGACTTC CAGGAGTGCC AGGAATGAAG GGAGAAGACG GTCTTCCAGG ACTCCCAGGA 
GTTACTGGAC TCAAGGGAGA TCTCGGAGCA CCAGGACAAT CCGGAGCACC AGGACTCCCA 
GGAGCACCAG GATACCCAGG AATGAAGGGA AATGCGGGTA TCCCAGGAGT GCCAGGATTC 
AAGGGAGACG GAGGACTCCC AGGACTGCCA GGACTTAACG GACCAAAGGG AGAACCAGGA 
GTACCAGGAA TGCCAGGAAC CCCAGGAATG AAGGGTAACG GTGGTCTGCC AGGATTGCCA 
GGACGTGATG GACTTTCCGG AGTACCAGGA ATGAAGGGAG ATCGTGGTTT CAACGGACTT 
CCAGGAGAGA AGGGAGAAGC TGGACCAGCT GCTCGTGACG GACAAAAGGG AGATGCCGGT 
CTTCCAGGAC AGCCAGGTCT TCGTGGACCA CAAGGACCAT CTGGACTTCC AGGTGTCCCA 
GGATTCAAGG GAGAGACTGG ACTTCCAGGA TATGGACAAC CAGGTCAACC AGGAGAGAAG 
GGACTTCCTG GCATTCCAGG AAAGGCTGGA CGTCAAGGAG CCCCAGGGTC ACCAGGACAA 
GACGGACTCC CAGGATTCCC AGGAATGAAG GGAGAATCTG GATACCCAGG ACAAGACGGC 
CTTCCAGGGC GTGATGGCCT CCCAGGAGTC CCAGGACAAA AGGGAGATCT TGGACAGTCT 
GGACAGCCAG GACTCTCCGG GGCCCCAGGA CTCGACGGTC AACCAGGAGT ACCAGGAATC 
CGTGGAGACA AGGGACAAGG AGGACTACCC GGAATCCCAG GAGATCGTGG AATGGATGGT 
TATCCAGGAC AAAAGGGAGA GAACGGTTAC CCAGGTCAGC CAGGTCTTCC AGGACTTGGT 
GGAGAGAAAG GATTCGCTGG AACCCCAGGT TTCCCAGGAC TTAAGGGATC CCCAGGATAC 
CCAGGACAAG ACGGACTTCC AGGAATCCCT GGATTGAAGG GAGACTCAGG ATTCCCAGGA 
CAGCCAGGAC AAGAAGGACT CCCAGGACTT TCCGGAGAGA AGGGAATGGG TGGACTCCCA 
GGAATGCCAG GACAACCAGG TCAATCTATT GCTGGACCAG TTGGACCACC AGGTGCCCCA 
GGACTTCAAG GAAAAGACGG CTTCCCAGGC CTTCCAGGAC AAAAGGGAGA ATCCGGTCTT 
TCAGGCCTTC CAGGAGCCCC TGGATTGAAG GGAGAATCCG GCATGCCAGG ATTCCCAGGA 
GCTAAGGGAG ATCTAGGAGC CAATGGAATT CCAGGAAAGC GCGGAGAAGA CGGTCTACCA 
GGAGTACCAG GAAGAGATGG ACAACCAGGA ATCCCAGGAT TGAAGGGAGA AGTTGGAGGT 
GCTGGTCTTC CAGGACAACC AGGCTTCCCA GGTATCCCAG GACTTAAGGG AGAAGGAGGC 
CTTCCAGGTT TCCCAGGAGC AAAGGGAGAA GCTGGATTCC CAGGTACCCC AGGAGTGCCA 
GGATACGCCG GAGAGAAGGG AGACGGAGGT CTTCCAGGTC TTCCAGGAAG AGACGGACTC 
CCAGGAGCTG ATGGACCAGT GGGACCACCA GGACCATCAG GACCACAAAA CCTCGTTGAG 
CCGGGAGAGA AGGGACTTCC AGGTCTTCCA GGAGCCCCAG GACTTCGTGG AGAGAAGGGA 
ATGCCAGGGC TCGATGGACC ACCAGGAAAC GATGGACCAC CAGGTCTTCC AGGACAACGT 
GGAAACGACG GATACCCAGG AGCCCCAGGA TTATCAGGAG AGAAGGGAAT GGGAGGTCTT 
CCAGGTTTCC CAGGACTTGA CGGACAACCA GGAGGCCCAG GAGCCCCAGG ATTGCCAGGA 
GCACCAGGAG CTGCAGGACC AGCTTACAGA GACGGATTCG TTCTCGTCAA GCACTCCCAA 
ACCACCGAAG TACCAAGATG CCCAGAGGGT CAGACCAAGC TGTGGGACGG ATATTCCTTG 
TTGTACATTG AAGGAAACGA GAAATCCCAT AACCAAGACC TCGGACACGC TGGATCATGC 
CTCCAAAGAT TCTCCACCAT GCCATTCCTG TTCTGCGACT TCAACAACGT CTGCAACTAC 
GCCTCCAGAA ACGACAAGTC TTACTGGCTC TCAACATCTG AAGCTATCCC AATGATGCCA 
GTCAACGAGC GTGAAATCGA GCCATACATC TCGCGCTGCG CTGTGTGCGA AGCCCCAGCC 
AACACGATCG CTGTTCACTC CCAGACCATC CAAATTCCAA ACTGCCCAGC AGGATGGAGC 
AGCTTGTGGA TCGGATACTC GTTCGCCATG CACACCGGAG CCGGAGCCGA GGGAGGTGGT 
CAATCACTCT CCTCACCAGG ATCCTGCTTG GAAGACTTCC GTGCCACTCC ATTCATCGAG 
TGCAACGGTG CCCGCGGATC TTGCCACTAC TTTGCCAACA AGTTCTCATT CTGGCTTACC 
ACCATCGACA ATGACTCAGA ATTCAAGGTG CCAGAAAGTC AGACACTCAA GTCAGGAAAT 
CTTCGTACCC GCGTCTCAAG ATGCCAAGTT TGCGTCAAGT CCACCGACGG ACGTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

let-2 ( NM_078263.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_510664.1 Caenorhabditis elegans Collagen alpha-2(IV) chain (let-2), partial mRNA.

Price & Availability↑

CloneID	OCa349689
Clone ID Related Accession (Same CDS sequence)	NM_078263.4 , NM_078263.3
Accession Version	NM_078263.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Collagen alpha-2(IV) chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_078263.2. COMPLETENESS: incomplete on both ends.

ATGAAGCAAC GAGCGGCTCT CGGGCCCGTG TTGCGACTGG CCATATTGGC CTTACTAGCT 
GTGAGCTATG TTCAATCGCA AGCTACATGC AGAGATTGCT CTAACCGAGG ATGTTTCTGC 
GTCGGAGAAA AAGGATCTAT GGGAGCACCA GGACCACAAG GTCCACCTGG AACCCAAGGA 
ATCCGTGGAT TCCCAGGACC AGAAGGACTC GCTGGACCAA AAGGACTGAA AGGAGCTCAA 
GGACCCCCTG GACCAGTTGG AATCAAGGGA GATCGTGGAG CCGTTGGAGT ACCTGGATTC 
CCAGGAAATG ACGGTGGAAA CGGACGACCA GGAGAGCCAG GACCACCAGG AGCCCCAGGA 
TGGGATGGTT GCAACGGAAC TGACGGAGCT CCAGGAATCC CAGGACGGCC AGGACCACCA 
GGAATGCCAG GATTCCCCGG ACCACCAGGA ATGGACGGTT TGAAGGGAGA GCCAGCTATC 
GGATATGCTG GTGCTCCAGG AGAGAAGGGA GATGGCGGTA TGCCAGGAAT GCCCGGACTT 
CCAGGACCAT CCGGTCGTGA TGGATACCCA GGAGAAAAGG GAGACCGAGG AGATACTGGA 
AATGCTGGAC CACGTGGACC ACCTGGAGAG GCTGGATCCC CAGGAAACCC AGGAATCGGA 
AGCATTGGAC CAAAAGGAGA TCCTGGAGAT CTAGGTTCTG TCGGACCACC AGGTCCACCG 
GGACCACGTG AGTTCACCGG ATCCGGCTCA ATTGTCGGAC CTCGCGGAAA CCCTGGAGAA 
AAGGGAGACA AGGGAGAGCC AGGAGAGGGA GGTCAACGCG GTTACCCAGG AAATGGAGGA 
CTCTCAGGAC AGCCAGGACT CCCAGGAATG AAGGGAGAAA AGGGATTGTC TGGACCAGCT 
GGACCAAGAG GAAAGGAAGG TCGCCCAGGA AACGCTGGAC CACCAGGATT CAAGGGAGAT 
CGTGGTCTTG ACGGACTTGG CGGAATCCCA GGACTTCCAG GCCAAAAGGG AGAAGCTGGA 
TACCCAGGAA GAGATGGGCC AAAGGGTAAC TCCGGACCAC CAGGACCACC AGGAGGTGGT 
ACTTTCAATG ACGGAGCTCC AGGACCACCA GGATTGCCAG GACGACCAGG AAACCCAGGA 
CCACCGGGAA CTGATGGCTA CCCAGGAGCC CCAGGGCCAG CTGGACCAAT CGGAAACACA 
GGAGGGCCAG GTCTTCCAGG TTACCCAGGA AATGAAGGAC TCCCAGGACC AAAGGGAGAT 
AAGGGAGATG GCGGTATTCC AGGAGCGCCA GGAGTCTCTG GACCATCTGG AATTCCAGGA 
CTTCCAGGAC CAAAGGGCGA ACCAGGATAT CGTGGAACCC CAGGACAAAG CATCCCAGGA 
CTTCCAGGAA AGGACGGAAA GCCAGGACTC GATGGAGCCC CAGGCCGCAA GGGAGAAAAT 
GGATTGCCAG GAGTTCGTGG ACCACCAGGA GATTCTCTTA ATGGACTTCC AGGAGCACCA 
GGACAACGCG GAGCACCAGG ACCAAACGGA TATGACGGAC GTGACGGAGT TAACGGTCTT 
CCGGGAGCCC CAGGAACAAA GGGAGACAGA GGAGGTACCT GCTCGGCTTG TGCTCCAGGA 
ACCAAGGGAG AAAAGGGATT GCCAGGATAT TCTGGTCAGC CAGGACCACA AGGAGACCGC 
GGTCTCCCAG GTATGCCAGG ACCAGTCGGA GACGCTGGAG ATGATGGTCT TCCGGGACCA 
GCAGGTCGCC CAGGATCTCC AGGACCACCA GGACAAGACG GTTTCCCAGG ACTCCCAGGA 
CAAAAGGGAG AACCAACTCA GCTAACCCTT AGACCAGGAC CACCAGGTTA TCCAGGATTG 
AAGGGAGAAA ACGGATTCCC AGGACAACCA GGAGTTGATG GACTCCCAGG ACCATCAGGG 
CCAGTCGGAC CACCAGGAGC GCCAGGATAC CCAGGAGAGA AGGGAGACGC CGGTCTTCCA 
GGACTTTCAG GAAAGCCAGG ACAGGACGGA CTTCCAGGAC TCCCAGGAAA TAAGGGAGAA 
GCCGGATACG GACAGCCAGG TCAACCAGGA TTCCCAGGAG CTAAAGGAGA TGGCGGTCTT 
CCAGGACTGC CAGGAACTCC AGGTCTCCAA GGAATGCCAG GAGAGCCAGC TCCAGAAAAC 
CAAGTGAACC CAGCTCCACC AGGTCAACCA GGACTCCCAG GACTTCCAGG AACCAAGGGA 
GAAGGAGGAT ACCCAGGTAG ACCAGGAGAA GTCGGTCAAC CAGGATTCCC AGGACTGCCA 
GGAATGAAGG GAGACTCTGG CCTCCCAGGA CCACCAGGAC TCCCAGGACA CCCAGGTGTT 
CCAGGAGACA AGGGATTCGG AGGTGTCCCA GGATTACCAG GAATTCCAGG ACCAAAGGGA 
GATGTCGGAA ACCCAGGACT CCCAGGTCTT AACGGACAAA AGGGAGAACC AGGAGTTGGA 
GTCCCAGGAC AACCAGGATC ACCAGGATTC CCAGGACTTA AGGGAGACGC CGGTCTTCCA 
GGTCTTCCAG GAACCCCAGG ACTCGAGGGT CAACGTGGAT TCCCAGGAGC CCCAGGACTC 
AAGGGAGGAG ACGGTCTTCC AGGACTGTCT GGTCAGCCAG GATACCCAGG AGAAAAGGGA 
GACGCCGGTT TGCCAGGAGT ACCAGGGCGT GAAGGATCGC CAGGATTCCC AGGTCAAGAT 
GGACTTCCAG GAGTGCCAGG AATGAAGGGA GAAGACGGTC TTCCAGGACT CCCAGGAGTT 
ACTGGACTCA AGGGAGATCT CGGAGCACCA GGACAATCCG GAGCACCAGG ACTCCCAGGA 
GCACCAGGAT ACCCAGGAAT GAAGGGAAAT GCGGGTATCC CAGGAGTGCC AGGATTCAAG 
GGAGACGGAG GACTCCCAGG ACTGCCAGGA CTTAACGGAC CAAAGGGAGA ACCAGGAGTA 
CCAGGAATGC CAGGAACCCC AGGAATGAAG GGTAACGGTG GTCTGCCAGG ATTGCCAGGA 
CGTGATGGAC TTTCCGGAGT ACCAGGAATG AAGGGAGATC GTGGTTTCAA CGGACTTCCA 
GGAGAGAAGG GAGAAGCTGG ACCAGCTGCT CGTGACGGAC AAAAGGGAGA TGCCGGTCTT 
CCAGGACAGC CAGGTCTTCG TGGACCACAA GGACCATCTG GACTTCCAGG TGTCCCAGGA 
TTCAAGGGAG AGACTGGACT TCCAGGATAT GGACAACCAG GTCAACCAGG AGAGAAGGGA 
CTTCCTGGCA TTCCAGGAAA GGCTGGACGT CAAGGAGCCC CAGGGTCACC AGGACAAGAC 
GGACTCCCAG GATTCCCAGG AATGAAGGGA GAATCTGGAT ACCCAGGACA AGACGGCCTT 
CCAGGGCGTG ATGGCCTCCC AGGAGTCCCA GGACAAAAGG GAGATCTTGG ACAGTCTGGA 
CAGCCAGGAC TCTCCGGGGC CCCAGGACTC GACGGTCAAC CAGGAGTACC AGGAATCCGT 
GGAGACAAGG GACAAGGAGG ACTACCCGGA ATCCCAGGAG ATCGTGGAAT GGATGGTTAT 
CCAGGACAAA AGGGAGAGAA CGGTTACCCA GGTCAGCCAG GTCTTCCAGG ACTTGGTGGA 
GAGAAAGGAT TCGCTGGAAC CCCAGGTTTC CCAGGACTTA AGGGATCCCC AGGATACCCA 
GGACAAGACG GACTTCCAGG AATCCCTGGA TTGAAGGGAG ACTCAGGATT CCCAGGACAG 
CCAGGACAAG AAGGACTCCC AGGACTTTCC GGAGAGAAGG GAATGGGTGG ACTCCCAGGA 
ATGCCAGGAC AACCAGGTCA ATCTATTGCT GGACCAGTTG GACCACCAGG TGCCCCAGGA 
CTTCAAGGAA AAGACGGCTT CCCAGGCCTT CCAGGACAAA AGGGAGAATC CGGTCTTTCA 
GGCCTTCCAG GAGCCCCTGG ATTGAAGGGA GAATCCGGCA TGCCAGGATT CCCAGGAGCT 
AAGGGAGATC TAGGAGCCAA TGGAATTCCA GGAAAGCGCG GAGAAGACGG TCTACCAGGA 
GTACCAGGAA GAGATGGACA ACCAGGAATC CCAGGATTGA AGGGAGAAGT TGGAGGTGCT 
GGTCTTCCAG GACAACCAGG CTTCCCAGGT ATCCCAGGAC TTAAGGGAGA AGGAGGCCTT 
CCAGGTTTCC CAGGAGCAAA GGGAGAAGCT GGATTCCCAG GTACCCCAGG AGTGCCAGGA 
TACGCCGGAG AGAAGGGAGA CGGAGGTCTT CCAGGTCTTC CAGGAAGAGA CGGACTCCCA 
GGAGCTGATG GACCAGTGGG ACCACCAGGA CCATCAGGAC CACAAAACCT CGTTGAGCCG 
GGAGAGAAGG GACTTCCAGG TCTTCCAGGA GCCCCAGGAC TTCGTGGAGA GAAGGGAATG 
CCAGGGCTCG ATGGACCACC AGGAAACGAT GGACCACCAG GTCTTCCAGG ACAACGTGGA 
AACGACGGAT ACCCAGGAGC CCCAGGATTA TCAGGAGAGA AGGGAATGGG AGGTCTTCCA 
GGTTTCCCAG GACTTGACGG ACAACCAGGA GGCCCAGGAG CCCCAGGATT GCCAGGAGCA 
CCAGGAGCTG CAGGACCAGC TTACAGAGAC GGATTCGTTC TCGTCAAGCA CTCCCAAACC 
ACCGAAGTAC CAAGATGCCC AGAGGGTCAG ACCAAGCTGT GGGACGGATA TTCCTTGTTG 
TACATTGAAG GAAACGAGAA ATCCCATAAC CAAGACCTCG GACACGCTGG ATCATGCCTC 
CAAAGATTCT CCACCATGCC ATTCCTGTTC TGCGACTTCA ACAACGTCTG CAACTACGCC 
TCCAGAAACG ACAAGTCTTA CTGGCTCTCA ACATCTGAAG CTATCCCAAT GATGCCAGTC 
AACGAGCGTG AAATCGAGCC ATACATCTCG CGCTGCGCTG TGTGCGAAGC CCCAGCCAAC 
ACGATCGCTG TTCACTCCCA GACCATCCAA ATTCCAAACT GCCCAGCAGG ATGGAGCAGC 
TTGTGGATCG GATACTCGTT CGCCATGCAC ACCGGAGCCG GAGCCGAGGG AGGTGGTCAA 
TCACTCTCCT CACCAGGATC CTGCTTGGAA GACTTCCGTG CCACTCCATT CATCGAGTGC 
AACGGTGCCC GCGGATCTTG CCACTACTTT GCCAACAAGT TCTCATTCTG GCTTACCACC 
ATCGACAATG ACTCAGAATT CAAGGTGCCA GAAAGTCAGA CACTCAAGTC AGGAAATCTT 
CGTACCCGCG TCTCAAGATG CCAAGTTTGC GTCAAGTCCA CCGACGGACG TCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_510664.1
CDS	1..5277
Translation	MKQRAALGPVLRLAILALLAVSYVQSQATCRDCSNRGCFCVGEKGSMGAPGPQGPPGTQGIRGFPGPEGLAGPKGLKGAQGPPGPVGIKGDRGAVGVPGFPGNDGGNGRPGEPGPPGAPGWDGCNGTDGAPGIPGRPGPPGMPGFPGPPGMDGLKGEPAIGYAGAPGEKGDGGMPGMPGLPGPSGRDGYPGEKGDRGDTGNAGPRGPPGEAGSPGNPGIGSIGPKGDPGDLGSVGPPGPPGPREFTGSGSIVGPRGNPGEKGDKGEPGEGGQRGYPGNGGLSGQPGLPGMKGEKGLSGPAGPRGKEGRPGNAGPPGFKGDRGLDGLGGIPGLPGQKGEAGYPGRDGPKGNSGPPGPPGGGTFNDGAPGPPGLPGRPGNPGPPGTDGYPGAPGPAGPIGNTGGPGLPGYPGNEGLPGPKGDKGDGGIPGAPGVSGPSGIPGLPGPKGEPGYRGTPGQSIPGLPGKDGKPGLDGAPGRKGENGLPGVRGPPGDSLNGLPGAPGQRGAPGPNGYDGRDGVNGLPGAPGTKGDRGGTCSACAPGTKGEKGLPGYSGQPGPQGDRGLPGMPGPVGDAGDDGLPGPAGRPGSPGPPGQDGFPGLPGQKGEPTQLTLRPGPPGYPGLKGENGFPGQPGVDGLPGPSGPVGPPGAPGYPGEKGDAGLPGLSGKPGQDGLPGLPGNKGEAGYGQPGQPGFPGAKGDGGLPGLPGTPGLQGMPGEPAPENQVNPAPPGQPGLPGLPGTKGEGGYPGRPGEVGQPGFPGLPGMKGDSGLPGPPGLPGHPGVPGDKGFGGVPGLPGIPGPKGDVGNPGLPGLNGQKGEPGVGVPGQPGSPGFPGLKGDAGLPGLPGTPGLEGQRGFPGAPGLKGGDGLPGLSGQPGYPGEKGDAGLPGVPGREGSPGFPGQDGLPGVPGMKGEDGLPGLPGVTGLKGDLGAPGQSGAPGLPGAPGYPGMKGNAGIPGVPGFKGDGGLPGLPGLNGPKGEPGVPGMPGTPGMKGNGGLPGLPGRDGLSGVPGMKGDRGFNGLPGEKGEAGPAARDGQKGDAGLPGQPGLRGPQGPSGLPGVPGFKGETGLPGYGQPGQPGEKGLPGIPGKAGRQGAPGSPGQDGLPGFPGMKGESGYPGQDGLPGRDGLPGVPGQKGDLGQSGQPGLSGAPGLDGQPGVPGIRGDKGQGGLPGIPGDRGMDGYPGQKGENGYPGQPGLPGLGGEKGFAGTPGFPGLKGSPGYPGQDGLPGIPGLKGDSGFPGQPGQEGLPGLSGEKGMGGLPGMPGQPGQSIAGPVGPPGAPGLQGKDGFPGLPGQKGESGLSGLPGAPGLKGESGMPGFPGAKGDLGANGIPGKRGEDGLPGVPGRDGQPGIPGLKGEVGGAGLPGQPGFPGIPGLKGEGGLPGFPGAKGEAGFPGTPGVPGYAGEKGDGGLPGLPGRDGLPGADGPVGPPGPSGPQNLVEPGEKGLPGLPGAPGLRGEKGMPGLDGPPGNDGPPGLPGQRGNDGYPGAPGLSGEKGMGGLPGFPGLDGQPGGPGAPGLPGAPGAAGPAYRDGFVLVKHSQTTEVPRCPEGQTKLWDGYSLLYIEGNEKSHNQDLGHAGSCLQRFSTMPFLFCDFNNVCNYASRNDKSYWLSTSEAIPMMPVNEREIEPYISRCAVCEAPANTIAVHSQTIQIPNCPAGWSSLWIGYSFAMHTGAGAEGGGQSLSSPGSCLEDFRATPFIECNGARGSCHYFANKFSFWLTTIDNDSEFKVPESQTLKSGNLRTRVSRCQVCVKSTDGRH

Target ORF information:

RefSeq Version	NM_078263.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Collagen alpha-2(IV) chain (let-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078263.3

ORF Insert Sequence:

ATGAAGCAAC GAGCGGCTCT CGGGCCCGTG TTGCGACTGG CCATATTGGC CTTACTAGCT 
GTGAGCTATG TTCAATCGCA AGCTACATGC AGAGATTGCT CTAACCGAGG ATGTTTCTGC 
GTCGGAGAAA AAGGATCTAT GGGAGCACCA GGACCACAAG GTCCACCTGG AACCCAAGGA 
ATCCGTGGAT TCCCAGGACC AGAAGGACTC GCTGGACCAA AAGGACTGAA AGGAGCTCAA 
GGACCCCCTG GACCAGTTGG AATCAAGGGA GATCGTGGAG CCGTTGGAGT ACCTGGATTC 
CCAGGAAATG ACGGTGGAAA CGGACGACCA GGAGAGCCAG GACCACCAGG AGCCCCAGGA 
TGGGATGGTT GCAACGGAAC TGACGGAGCT CCAGGAATCC CAGGACGGCC AGGACCACCA 
GGAATGCCAG GATTCCCCGG ACCACCAGGA ATGGACGGTT TGAAGGGAGA GCCAGCTATC 
GGATATGCTG GTGCTCCAGG AGAGAAGGGA GATGGCGGTA TGCCAGGAAT GCCCGGACTT 
CCAGGACCAT CCGGTCGTGA TGGATACCCA GGAGAAAAGG GAGACCGAGG AGATACTGGA 
AATGCTGGAC CACGTGGACC ACCTGGAGAG GCTGGATCCC CAGGAAACCC AGGAATCGGA 
AGCATTGGAC CAAAAGGAGA TCCTGGAGAT CTAGGTTCTG TCGGACCACC AGGTCCACCG 
GGACCACGTG AGTTCACCGG ATCCGGCTCA ATTGTCGGAC CTCGCGGAAA CCCTGGAGAA 
AAGGGAGACA AGGGAGAGCC AGGAGAGGGA GGTCAACGCG GTTACCCAGG AAATGGAGGA 
CTCTCAGGAC AGCCAGGACT CCCAGGAATG AAGGGAGAAA AGGGATTGTC TGGACCAGCT 
GGACCAAGAG GAAAGGAAGG TCGCCCAGGA AACGCTGGAC CACCAGGATT CAAGGGAGAT 
CGTGGTCTTG ACGGACTTGG CGGAATCCCA GGACTTCCAG GCCAAAAGGG AGAAGCTGGA 
TACCCAGGAA GAGATGGGCC AAAGGGTAAC TCCGGACCAC CAGGACCACC AGGAGGTGGT 
ACTTTCAATG ACGGAGCTCC AGGACCACCA GGATTGCCAG GACGACCAGG AAACCCAGGA 
CCACCGGGAA CTGATGGCTA CCCAGGAGCC CCAGGGCCAG CTGGACCAAT CGGAAACACA 
GGAGGGCCAG GTCTTCCAGG TTACCCAGGA AATGAAGGAC TCCCAGGACC AAAGGGAGAT 
AAGGGAGATG GCGGTATTCC AGGAGCGCCA GGAGTCTCTG GACCATCTGG AATTCCAGGA 
CTTCCAGGAC CAAAGGGCGA ACCAGGATAT CGTGGAACCC CAGGACAAAG CATCCCAGGA 
CTTCCAGGAA AGGACGGAAA GCCAGGACTC GATGGAGCCC CAGGCCGCAA GGGAGAAAAT 
GGATTGCCAG GAGTTCGTGG ACCACCAGGA GATTCTCTTA ATGGACTTCC AGGAGCACCA 
GGACAACGCG GAGCACCAGG ACCAAACGGA TATGACGGAC GTGACGGAGT TAACGGTCTT 
CCGGGAGCCC CAGGAACAAA GGGAGACAGA GGAGGTACCT GCTCGGCTTG TGCTCCAGGA 
ACCAAGGGAG AAAAGGGATT GCCAGGATAT TCTGGTCAGC CAGGACCACA AGGAGACCGC 
GGTCTCCCAG GTATGCCAGG ACCAGTCGGA GACGCTGGAG ATGATGGTCT TCCGGGACCA 
GCAGGTCGCC CAGGATCTCC AGGACCACCA GGACAAGACG GTTTCCCAGG ACTCCCAGGA 
CAAAAGGGAG AACCAACTCA GCTAACCCTT AGACCAGGAC CACCAGGTTA TCCAGGATTG 
AAGGGAGAAA ACGGATTCCC AGGACAACCA GGAGTTGATG GACTCCCAGG ACCATCAGGG 
CCAGTCGGAC CACCAGGAGC GCCAGGATAC CCAGGAGAGA AGGGAGACGC CGGTCTTCCA 
GGACTTTCAG GAAAGCCAGG ACAGGACGGA CTTCCAGGAC TCCCAGGAAA TAAGGGAGAA 
GCCGGATACG GACAGCCAGG TCAACCAGGA TTCCCAGGAG CTAAAGGAGA TGGCGGTCTT 
CCAGGACTGC CAGGAACTCC AGGTCTCCAA GGAATGCCAG GAGAGCCAGC TCCAGAAAAC 
CAAGTGAACC CAGCTCCACC AGGTCAACCA GGACTCCCAG GACTTCCAGG AACCAAGGGA 
GAAGGAGGAT ACCCAGGTAG ACCAGGAGAA GTCGGTCAAC CAGGATTCCC AGGACTGCCA 
GGAATGAAGG GAGACTCTGG CCTCCCAGGA CCACCAGGAC TCCCAGGACA CCCAGGTGTT 
CCAGGAGACA AGGGATTCGG AGGTGTCCCA GGATTACCAG GAATTCCAGG ACCAAAGGGA 
GATGTCGGAA ACCCAGGACT CCCAGGTCTT AACGGACAAA AGGGAGAACC AGGAGTTGGA 
GTCCCAGGAC AACCAGGATC ACCAGGATTC CCAGGACTTA AGGGAGACGC CGGTCTTCCA 
GGTCTTCCAG GAACCCCAGG ACTCGAGGGT CAACGTGGAT TCCCAGGAGC CCCAGGACTC 
AAGGGAGGAG ACGGTCTTCC AGGACTGTCT GGTCAGCCAG GATACCCAGG AGAAAAGGGA 
GACGCCGGTT TGCCAGGAGT ACCAGGGCGT GAAGGATCGC CAGGATTCCC AGGTCAAGAT 
GGACTTCCAG GAGTGCCAGG AATGAAGGGA GAAGACGGTC TTCCAGGACT CCCAGGAGTT 
ACTGGACTCA AGGGAGATCT CGGAGCACCA GGACAATCCG GAGCACCAGG ACTCCCAGGA 
GCACCAGGAT ACCCAGGAAT GAAGGGAAAT GCGGGTATCC CAGGAGTGCC AGGATTCAAG 
GGAGACGGAG GACTCCCAGG ACTGCCAGGA CTTAACGGAC CAAAGGGAGA ACCAGGAGTA 
CCAGGAATGC CAGGAACCCC AGGAATGAAG GGTAACGGTG GTCTGCCAGG ATTGCCAGGA 
CGTGATGGAC TTTCCGGAGT ACCAGGAATG AAGGGAGATC GTGGTTTCAA CGGACTTCCA 
GGAGAGAAGG GAGAAGCTGG ACCAGCTGCT CGTGACGGAC AAAAGGGAGA TGCCGGTCTT 
CCAGGACAGC CAGGTCTTCG TGGACCACAA GGACCATCTG GACTTCCAGG TGTCCCAGGA 
TTCAAGGGAG AGACTGGACT TCCAGGATAT GGACAACCAG GTCAACCAGG AGAGAAGGGA 
CTTCCTGGCA TTCCAGGAAA GGCTGGACGT CAAGGAGCCC CAGGGTCACC AGGACAAGAC 
GGACTCCCAG GATTCCCAGG AATGAAGGGA GAATCTGGAT ACCCAGGACA AGACGGCCTT 
CCAGGGCGTG ATGGCCTCCC AGGAGTCCCA GGACAAAAGG GAGATCTTGG ACAGTCTGGA 
CAGCCAGGAC TCTCCGGGGC CCCAGGACTC GACGGTCAAC CAGGAGTACC AGGAATCCGT 
GGAGACAAGG GACAAGGAGG ACTACCCGGA ATCCCAGGAG ATCGTGGAAT GGATGGTTAT 
CCAGGACAAA AGGGAGAGAA CGGTTACCCA GGTCAGCCAG GTCTTCCAGG ACTTGGTGGA 
GAGAAAGGAT TCGCTGGAAC CCCAGGTTTC CCAGGACTTA AGGGATCCCC AGGATACCCA 
GGACAAGACG GACTTCCAGG AATCCCTGGA TTGAAGGGAG ACTCAGGATT CCCAGGACAG 
CCAGGACAAG AAGGACTCCC AGGACTTTCC GGAGAGAAGG GAATGGGTGG ACTCCCAGGA 
ATGCCAGGAC AACCAGGTCA ATCTATTGCT GGACCAGTTG GACCACCAGG TGCCCCAGGA 
CTTCAAGGAA AAGACGGCTT CCCAGGCCTT CCAGGACAAA AGGGAGAATC CGGTCTTTCA 
GGCCTTCCAG GAGCCCCTGG ATTGAAGGGA GAATCCGGCA TGCCAGGATT CCCAGGAGCT 
AAGGGAGATC TAGGAGCCAA TGGAATTCCA GGAAAGCGCG GAGAAGACGG TCTACCAGGA 
GTACCAGGAA GAGATGGACA ACCAGGAATC CCAGGATTGA AGGGAGAAGT TGGAGGTGCT 
GGTCTTCCAG GACAACCAGG CTTCCCAGGT ATCCCAGGAC TTAAGGGAGA AGGAGGCCTT 
CCAGGTTTCC CAGGAGCAAA GGGAGAAGCT GGATTCCCAG GTACCCCAGG AGTGCCAGGA 
TACGCCGGAG AGAAGGGAGA CGGAGGTCTT CCAGGTCTTC CAGGAAGAGA CGGACTCCCA 
GGAGCTGATG GACCAGTGGG ACCACCAGGA CCATCAGGAC CACAAAACCT CGTTGAGCCG 
GGAGAGAAGG GACTTCCAGG TCTTCCAGGA GCCCCAGGAC TTCGTGGAGA GAAGGGAATG 
CCAGGGCTCG ATGGACCACC AGGAAACGAT GGACCACCAG GTCTTCCAGG ACAACGTGGA 
AACGACGGAT ACCCAGGAGC CCCAGGATTA TCAGGAGAGA AGGGAATGGG AGGTCTTCCA 
GGTTTCCCAG GACTTGACGG ACAACCAGGA GGCCCAGGAG CCCCAGGATT GCCAGGAGCA 
CCAGGAGCTG CAGGACCAGC TTACAGAGAC GGATTCGTTC TCGTCAAGCA CTCCCAAACC 
ACCGAAGTAC CAAGATGCCC AGAGGGTCAG ACCAAGCTGT GGGACGGATA TTCCTTGTTG 
TACATTGAAG GAAACGAGAA ATCCCATAAC CAAGACCTCG GACACGCTGG ATCATGCCTC 
CAAAGATTCT CCACCATGCC ATTCCTGTTC TGCGACTTCA ACAACGTCTG CAACTACGCC 
TCCAGAAACG ACAAGTCTTA CTGGCTCTCA ACATCTGAAG CTATCCCAAT GATGCCAGTC 
AACGAGCGTG AAATCGAGCC ATACATCTCG CGCTGCGCTG TGTGCGAAGC CCCAGCCAAC 
ACGATCGCTG TTCACTCCCA GACCATCCAA ATTCCAAACT GCCCAGCAGG ATGGAGCAGC 
TTGTGGATCG GATACTCGTT CGCCATGCAC ACCGGAGCCG GAGCCGAGGG AGGTGGTCAA 
TCACTCTCCT CACCAGGATC CTGCTTGGAA GACTTCCGTG CCACTCCATT CATCGAGTGC 
AACGGTGCCC GCGGATCTTG CCACTACTTT GCCAACAAGT TCTCATTCTG GCTTACCACC 
ATCGACAATG ACTCAGAATT CAAGGTGCCA GAAAGTCAGA CACTCAAGTC AGGAAATCTT 
CGTACCCGCG TCTCAAGATG CCAAGTTTGC GTCAAGTCCA CCGACGGACG TCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

let-2 ( NM_078262.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_510663.1 Caenorhabditis elegans Collagen alpha-2(IV) chain (let-2), partial mRNA.

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CloneID	OCa349690
Clone ID Related Accession (Same CDS sequence)	NM_078262.4 , NM_078262.5
Accession Version	NM_078262.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Collagen alpha-2(IV) chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_078262.3. COMPLETENESS: incomplete on both ends.

ATGAAGCAAC GAGCGGCTCT CGGGCCCGTG TTGCGACTGG CCATATTGGC CTTACTAGCT 
GTGAGCTATG TTCAATCGCA AGCTACATGC AGAGATTGCT CTAACCGAGG ATGTTTCTGC 
GTCGGAGAAA AAGGATCTAT GGGAGCACCA GGACCACAAG GTCCACCTGG AACCCAAGGA 
ATCCGTGGAT TCCCAGGACC AGAAGGACTC GCTGGACCAA AAGGACTGAA AGGAGCTCAA 
GGACCCCCTG GACCAGTTGG AATCAAGGGA GATCGTGGAG CCGTTGGAGT ACCTGGATTC 
CCAGGAAATG ACGGTGGAAA CGGACGACCA GGAGAGCCAG GACCACCAGG AGCCCCAGGA 
TGGGATGGTT GCAACGGAAC TGACGGAGCT CCAGGAATCC CAGGACGGCC AGGACCACCA 
GGAATGCCAG GATTCCCCGG ACCACCAGGA ATGGACGGTT TGAAGGGAGA GCCAGCTATC 
GGATATGCTG GTGCTCCAGG AGAGAAGGGA GATGGCGGTA TGCCAGGAAT GCCCGGACTT 
CCAGGACCAT CCGGTCGTGA TGGATACCCA GGAGAAAAGG GAGACCGAGG AGATACTGGA 
AATGCTGGAC CACGTGGACC ACCTGGAGAG GCTGGATCCC CAGGAAACCC AGGAATCGGA 
AGCATTGGAC CAAAAGGAGA TCCTGGAGAC ATTGGTGCGA TGGGACCGGC CGGTCCGCCA 
GGCCCAATCG CCTCCACCAT GTCCAAGGGA ACCATTATCG GTCCTAAGGG AGACCTAGGA 
GAGAAGGGAG AGAAGGGAGA GCCAGGAGAG GGAGGTCAAC GCGGTTACCC AGGAAATGGA 
GGACTCTCAG GACAGCCAGG ACTCCCAGGA ATGAAGGGAG AAAAGGGATT GTCTGGACCA 
GCTGGACCAA GAGGAAAGGA AGGTCGCCCA GGAAACGCTG GACCACCAGG ATTCAAGGGA 
GATCGTGGTC TTGACGGACT TGGCGGAATC CCAGGACTTC CAGGCCAAAA GGGAGAAGCT 
GGATACCCAG GAAGAGATGG GCCAAAGGGT AACTCCGGAC CACCAGGACC ACCAGGAGGT 
GGTACTTTCA ATGACGGAGC TCCAGGACCA CCAGGATTGC CAGGACGACC AGGAAACCCA 
GGACCACCGG GAACTGATGG CTACCCAGGA GCCCCAGGGC CAGCTGGACC AATCGGAAAC 
ACAGGAGGGC CAGGTCTTCC AGGTTACCCA GGAAATGAAG GACTCCCAGG ACCAAAGGGA 
GATAAGGGAG ATGGCGGTAT TCCAGGAGCG CCAGGAGTCT CTGGACCATC TGGAATTCCA 
GGACTTCCAG GACCAAAGGG CGAACCAGGA TATCGTGGAA CCCCAGGACA AAGCATCCCA 
GGACTTCCAG GAAAGGACGG AAAGCCAGGA CTCGATGGAG CCCCAGGCCG CAAGGGAGAA 
AATGGATTGC CAGGAGTTCG TGGACCACCA GGAGATTCTC TTAATGGACT TCCAGGAGCA 
CCAGGACAAC GCGGAGCACC AGGACCAAAC GGATATGACG GACGTGACGG AGTTAACGGT 
CTTCCGGGAG CCCCAGGAAC AAAGGGAGAC AGAGGAGGTA CCTGCTCGGC TTGTGCTCCA 
GGAACCAAGG GAGAAAAGGG ATTGCCAGGA TATTCTGGTC AGCCAGGACC ACAAGGAGAC 
CGCGGTCTCC CAGGTATGCC AGGACCAGTC GGAGACGCTG GAGATGATGG TCTTCCGGGA 
CCAGCAGGTC GCCCAGGATC TCCAGGACCA CCAGGACAAG ACGGTTTCCC AGGACTCCCA 
GGACAAAAGG GAGAACCAAC TCAGCTAACC CTTAGACCAG GACCACCAGG TTATCCAGGA 
TTGAAGGGAG AAAACGGATT CCCAGGACAA CCAGGAGTTG ATGGACTCCC AGGACCATCA 
GGGCCAGTCG GACCACCAGG AGCGCCAGGA TACCCAGGAG AGAAGGGAGA CGCCGGTCTT 
CCAGGACTTT CAGGAAAGCC AGGACAGGAC GGACTTCCAG GACTCCCAGG AAATAAGGGA 
GAAGCCGGAT ACGGACAGCC AGGTCAACCA GGATTCCCAG GAGCTAAAGG AGATGGCGGT 
CTTCCAGGAC TGCCAGGAAC TCCAGGTCTC CAAGGAATGC CAGGAGAGCC AGCTCCAGAA 
AACCAAGTGA ACCCAGCTCC ACCAGGTCAA CCAGGACTCC CAGGACTTCC AGGAACCAAG 
GGAGAAGGAG GATACCCAGG TAGACCAGGA GAAGTCGGTC AACCAGGATT CCCAGGACTG 
CCAGGAATGA AGGGAGACTC TGGCCTCCCA GGACCACCAG GACTCCCAGG ACACCCAGGT 
GTTCCAGGAG ACAAGGGATT CGGAGGTGTC CCAGGATTAC CAGGAATTCC AGGACCAAAG 
GGAGATGTCG GAAACCCAGG ACTCCCAGGT CTTAACGGAC AAAAGGGAGA ACCAGGAGTT 
GGAGTCCCAG GACAACCAGG ATCACCAGGA TTCCCAGGAC TTAAGGGAGA CGCCGGTCTT 
CCAGGTCTTC CAGGAACCCC AGGACTCGAG GGTCAACGTG GATTCCCAGG AGCCCCAGGA 
CTCAAGGGAG GAGACGGTCT TCCAGGACTG TCTGGTCAGC CAGGATACCC AGGAGAAAAG 
GGAGACGCCG GTTTGCCAGG AGTACCAGGG CGTGAAGGAT CGCCAGGATT CCCAGGTCAA 
GATGGACTTC CAGGAGTGCC AGGAATGAAG GGAGAAGACG GTCTTCCAGG ACTCCCAGGA 
GTTACTGGAC TCAAGGGAGA TCTCGGAGCA CCAGGACAAT CCGGAGCACC AGGACTCCCA 
GGAGCACCAG GATACCCAGG AATGAAGGGA AATGCGGGTA TCCCAGGAGT GCCAGGATTC 
AAGGGAGACG GAGGACTCCC AGGACTGCCA GGACTTAACG GACCAAAGGG AGAACCAGGA 
GTACCAGGAA TGCCAGGAAC CCCAGGAATG AAGGGTAACG GTGGTCTGCC AGGATTGCCA 
GGACGTGATG GACTTTCCGG AGTACCAGGA ATGAAGGGAG ATCGTGGTTT CAACGGACTT 
CCAGGAGAGA AGGGAGAAGC TGGACCAGCT GCTCGTGACG GACAAAAGGG AGATGCCGGT 
CTTCCAGGAC AGCCAGGTCT TCGTGGACCA CAAGGACCAT CTGGACTTCC AGGTGTCCCA 
GGATTCAAGG GAGAGACTGG ACTTCCAGGA TATGGACAAC CAGGTCAACC AGGAGAGAAG 
GGACTTCCTG GCATTCCAGG AAAGGCTGGA CGTCAAGGAG CCCCAGGGTC ACCAGGACAA 
GACGGACTCC CAGGATTCCC AGGAATGAAG GGAGAATCTG GATACCCAGG ACAAGACGGC 
CTTCCAGGGC GTGATGGCCT CCCAGGAGTC CCAGGACAAA AGGGAGATCT TGGACAGTCT 
GGACAGCCAG GACTCTCCGG GGCCCCAGGA CTCGACGGTC AACCAGGAGT ACCAGGAATC 
CGTGGAGACA AGGGACAAGG AGGACTACCC GGAATCCCAG GAGATCGTGG AATGGATGGT 
TATCCAGGAC AAAAGGGAGA GAACGGTTAC CCAGGTCAGC CAGGTCTTCC AGGACTTGGT 
GGAGAGAAAG GATTCGCTGG AACCCCAGGT TTCCCAGGAC TTAAGGGATC CCCAGGATAC 
CCAGGACAAG ACGGACTTCC AGGAATCCCT GGATTGAAGG GAGACTCAGG ATTCCCAGGA 
CAGCCAGGAC AAGAAGGACT CCCAGGACTT TCCGGAGAGA AGGGAATGGG TGGACTCCCA 
GGAATGCCAG GACAACCAGG TCAATCTATT GCTGGACCAG TTGGACCACC AGGTGCCCCA 
GGACTTCAAG GAAAAGACGG CTTCCCAGGC CTTCCAGGAC AAAAGGGAGA ATCCGGTCTT 
TCAGGCCTTC CAGGAGCCCC TGGATTGAAG GGAGAATCCG GCATGCCAGG ATTCCCAGGA 
GCTAAGGGAG ATCTAGGAGC CAATGGAATT CCAGGAAAGC GCGGAGAAGA CGGTCTACCA 
GGAGTACCAG GAAGAGATGG ACAACCAGGA ATCCCAGGAT TGAAGGGAGA AGTTGGAGGT 
GCTGGTCTTC CAGGACAACC AGGCTTCCCA GGTATCCCAG GACTTAAGGG AGAAGGAGGC 
CTTCCAGGTT TCCCAGGAGC AAAGGGAGAA GCTGGATTCC CAGGTACCCC AGGAGTGCCA 
GGATACGCCG GAGAGAAGGG AGACGGAGGT CTTCCAGGTC TTCCAGGAAG AGACGGACTC 
CCAGGAGCTG ATGGACCAGT GGGACCACCA GGACCATCAG GACCACAAAA CCTCGTTGAG 
CCGGGAGAGA AGGGACTTCC AGGTCTTCCA GGAGCCCCAG GACTTCGTGG AGAGAAGGGA 
ATGCCAGGGC TCGATGGACC ACCAGGAAAC GATGGACCAC CAGGTCTTCC AGGACAACGT 
GGAAACGACG GATACCCAGG AGCCCCAGGA TTATCAGGAG AGAAGGGAAT GGGAGGTCTT 
CCAGGTTTCC CAGGACTTGA CGGACAACCA GGAGGCCCAG GAGCCCCAGG ATTGCCAGGA 
GCACCAGGAG CTGCAGGACC AGCTTACAGA GACGGATTCG TTCTCGTCAA GCACTCCCAA 
ACCACCGAAG TACCAAGATG CCCAGAGGGT CAGACCAAGC TGTGGGACGG ATATTCCTTG 
TTGTACATTG AAGGAAACGA GAAATCCCAT AACCAAGACC TCGGACACGC TGGATCATGC 
CTCCAAAGAT TCTCCACCAT GCCATTCCTG TTCTGCGACT TCAACAACGT CTGCAACTAC 
GCCTCCAGAA ACGACAAGTC TTACTGGCTC TCAACATCTG AAGCTATCCC AATGATGCCA 
GTCAACGAGC GTGAAATCGA GCCATACATC TCGCGCTGCG CTGTGTGCGA AGCCCCAGCC 
AACACGATCG CTGTTCACTC CCAGACCATC CAAATTCCAA ACTGCCCAGC AGGATGGAGC 
AGCTTGTGGA TCGGATACTC GTTCGCCATG CACACCGGAG CCGGAGCCGA GGGAGGTGGT 
CAATCACTCT CCTCACCAGG ATCCTGCTTG GAAGACTTCC GTGCCACTCC ATTCATCGAG 
TGCAACGGTG CCCGCGGATC TTGCCACTAC TTTGCCAACA AGTTCTCATT CTGGCTTACC 
ACCATCGACA ATGACTCAGA ATTCAAGGTG CCAGAAAGTC AGACACTCAA GTCAGGAAAT 
CTTCGTACCC GCGTCTCAAG ATGCCAAGTT TGCGTCAAGT CCACCGACGG ACGTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_510663.1
CDS	1..5280
Translation	MKQRAALGPVLRLAILALLAVSYVQSQATCRDCSNRGCFCVGEKGSMGAPGPQGPPGTQGIRGFPGPEGLAGPKGLKGAQGPPGPVGIKGDRGAVGVPGFPGNDGGNGRPGEPGPPGAPGWDGCNGTDGAPGIPGRPGPPGMPGFPGPPGMDGLKGEPAIGYAGAPGEKGDGGMPGMPGLPGPSGRDGYPGEKGDRGDTGNAGPRGPPGEAGSPGNPGIGSIGPKGDPGDIGAMGPAGPPGPIASTMSKGTIIGPKGDLGEKGEKGEPGEGGQRGYPGNGGLSGQPGLPGMKGEKGLSGPAGPRGKEGRPGNAGPPGFKGDRGLDGLGGIPGLPGQKGEAGYPGRDGPKGNSGPPGPPGGGTFNDGAPGPPGLPGRPGNPGPPGTDGYPGAPGPAGPIGNTGGPGLPGYPGNEGLPGPKGDKGDGGIPGAPGVSGPSGIPGLPGPKGEPGYRGTPGQSIPGLPGKDGKPGLDGAPGRKGENGLPGVRGPPGDSLNGLPGAPGQRGAPGPNGYDGRDGVNGLPGAPGTKGDRGGTCSACAPGTKGEKGLPGYSGQPGPQGDRGLPGMPGPVGDAGDDGLPGPAGRPGSPGPPGQDGFPGLPGQKGEPTQLTLRPGPPGYPGLKGENGFPGQPGVDGLPGPSGPVGPPGAPGYPGEKGDAGLPGLSGKPGQDGLPGLPGNKGEAGYGQPGQPGFPGAKGDGGLPGLPGTPGLQGMPGEPAPENQVNPAPPGQPGLPGLPGTKGEGGYPGRPGEVGQPGFPGLPGMKGDSGLPGPPGLPGHPGVPGDKGFGGVPGLPGIPGPKGDVGNPGLPGLNGQKGEPGVGVPGQPGSPGFPGLKGDAGLPGLPGTPGLEGQRGFPGAPGLKGGDGLPGLSGQPGYPGEKGDAGLPGVPGREGSPGFPGQDGLPGVPGMKGEDGLPGLPGVTGLKGDLGAPGQSGAPGLPGAPGYPGMKGNAGIPGVPGFKGDGGLPGLPGLNGPKGEPGVPGMPGTPGMKGNGGLPGLPGRDGLSGVPGMKGDRGFNGLPGEKGEAGPAARDGQKGDAGLPGQPGLRGPQGPSGLPGVPGFKGETGLPGYGQPGQPGEKGLPGIPGKAGRQGAPGSPGQDGLPGFPGMKGESGYPGQDGLPGRDGLPGVPGQKGDLGQSGQPGLSGAPGLDGQPGVPGIRGDKGQGGLPGIPGDRGMDGYPGQKGENGYPGQPGLPGLGGEKGFAGTPGFPGLKGSPGYPGQDGLPGIPGLKGDSGFPGQPGQEGLPGLSGEKGMGGLPGMPGQPGQSIAGPVGPPGAPGLQGKDGFPGLPGQKGESGLSGLPGAPGLKGESGMPGFPGAKGDLGANGIPGKRGEDGLPGVPGRDGQPGIPGLKGEVGGAGLPGQPGFPGIPGLKGEGGLPGFPGAKGEAGFPGTPGVPGYAGEKGDGGLPGLPGRDGLPGADGPVGPPGPSGPQNLVEPGEKGLPGLPGAPGLRGEKGMPGLDGPPGNDGPPGLPGQRGNDGYPGAPGLSGEKGMGGLPGFPGLDGQPGGPGAPGLPGAPGAAGPAYRDGFVLVKHSQTTEVPRCPEGQTKLWDGYSLLYIEGNEKSHNQDLGHAGSCLQRFSTMPFLFCDFNNVCNYASRNDKSYWLSTSEAIPMMPVNEREIEPYISRCAVCEAPANTIAVHSQTIQIPNCPAGWSSLWIGYSFAMHTGAGAEGGGQSLSSPGSCLEDFRATPFIECNGARGSCHYFANKFSFWLTTIDNDSEFKVPESQTLKSGNLRTRVSRCQVCVKSTDGRH

Target ORF information:

RefSeq Version	NM_078262.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Collagen alpha-2(IV) chain (let-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078262.4

ORF Insert Sequence:

ATGAAGCAAC GAGCGGCTCT CGGGCCCGTG TTGCGACTGG CCATATTGGC CTTACTAGCT 
GTGAGCTATG TTCAATCGCA AGCTACATGC AGAGATTGCT CTAACCGAGG ATGTTTCTGC 
GTCGGAGAAA AAGGATCTAT GGGAGCACCA GGACCACAAG GTCCACCTGG AACCCAAGGA 
ATCCGTGGAT TCCCAGGACC AGAAGGACTC GCTGGACCAA AAGGACTGAA AGGAGCTCAA 
GGACCCCCTG GACCAGTTGG AATCAAGGGA GATCGTGGAG CCGTTGGAGT ACCTGGATTC 
CCAGGAAATG ACGGTGGAAA CGGACGACCA GGAGAGCCAG GACCACCAGG AGCCCCAGGA 
TGGGATGGTT GCAACGGAAC TGACGGAGCT CCAGGAATCC CAGGACGGCC AGGACCACCA 
GGAATGCCAG GATTCCCCGG ACCACCAGGA ATGGACGGTT TGAAGGGAGA GCCAGCTATC 
GGATATGCTG GTGCTCCAGG AGAGAAGGGA GATGGCGGTA TGCCAGGAAT GCCCGGACTT 
CCAGGACCAT CCGGTCGTGA TGGATACCCA GGAGAAAAGG GAGACCGAGG AGATACTGGA 
AATGCTGGAC CACGTGGACC ACCTGGAGAG GCTGGATCCC CAGGAAACCC AGGAATCGGA 
AGCATTGGAC CAAAAGGAGA TCCTGGAGAC ATTGGTGCGA TGGGACCGGC CGGTCCGCCA 
GGCCCAATCG CCTCCACCAT GTCCAAGGGA ACCATTATCG GTCCTAAGGG AGACCTAGGA 
GAGAAGGGAG AGAAGGGAGA GCCAGGAGAG GGAGGTCAAC GCGGTTACCC AGGAAATGGA 
GGACTCTCAG GACAGCCAGG ACTCCCAGGA ATGAAGGGAG AAAAGGGATT GTCTGGACCA 
GCTGGACCAA GAGGAAAGGA AGGTCGCCCA GGAAACGCTG GACCACCAGG ATTCAAGGGA 
GATCGTGGTC TTGACGGACT TGGCGGAATC CCAGGACTTC CAGGCCAAAA GGGAGAAGCT 
GGATACCCAG GAAGAGATGG GCCAAAGGGT AACTCCGGAC CACCAGGACC ACCAGGAGGT 
GGTACTTTCA ATGACGGAGC TCCAGGACCA CCAGGATTGC CAGGACGACC AGGAAACCCA 
GGACCACCGG GAACTGATGG CTACCCAGGA GCCCCAGGGC CAGCTGGACC AATCGGAAAC 
ACAGGAGGGC CAGGTCTTCC AGGTTACCCA GGAAATGAAG GACTCCCAGG ACCAAAGGGA 
GATAAGGGAG ATGGCGGTAT TCCAGGAGCG CCAGGAGTCT CTGGACCATC TGGAATTCCA 
GGACTTCCAG GACCAAAGGG CGAACCAGGA TATCGTGGAA CCCCAGGACA AAGCATCCCA 
GGACTTCCAG GAAAGGACGG AAAGCCAGGA CTCGATGGAG CCCCAGGCCG CAAGGGAGAA 
AATGGATTGC CAGGAGTTCG TGGACCACCA GGAGATTCTC TTAATGGACT TCCAGGAGCA 
CCAGGACAAC GCGGAGCACC AGGACCAAAC GGATATGACG GACGTGACGG AGTTAACGGT 
CTTCCGGGAG CCCCAGGAAC AAAGGGAGAC AGAGGAGGTA CCTGCTCGGC TTGTGCTCCA 
GGAACCAAGG GAGAAAAGGG ATTGCCAGGA TATTCTGGTC AGCCAGGACC ACAAGGAGAC 
CGCGGTCTCC CAGGTATGCC AGGACCAGTC GGAGACGCTG GAGATGATGG TCTTCCGGGA 
CCAGCAGGTC GCCCAGGATC TCCAGGACCA CCAGGACAAG ACGGTTTCCC AGGACTCCCA 
GGACAAAAGG GAGAACCAAC TCAGCTAACC CTTAGACCAG GACCACCAGG TTATCCAGGA 
TTGAAGGGAG AAAACGGATT CCCAGGACAA CCAGGAGTTG ATGGACTCCC AGGACCATCA 
GGGCCAGTCG GACCACCAGG AGCGCCAGGA TACCCAGGAG AGAAGGGAGA CGCCGGTCTT 
CCAGGACTTT CAGGAAAGCC AGGACAGGAC GGACTTCCAG GACTCCCAGG AAATAAGGGA 
GAAGCCGGAT ACGGACAGCC AGGTCAACCA GGATTCCCAG GAGCTAAAGG AGATGGCGGT 
CTTCCAGGAC TGCCAGGAAC TCCAGGTCTC CAAGGAATGC CAGGAGAGCC AGCTCCAGAA 
AACCAAGTGA ACCCAGCTCC ACCAGGTCAA CCAGGACTCC CAGGACTTCC AGGAACCAAG 
GGAGAAGGAG GATACCCAGG TAGACCAGGA GAAGTCGGTC AACCAGGATT CCCAGGACTG 
CCAGGAATGA AGGGAGACTC TGGCCTCCCA GGACCACCAG GACTCCCAGG ACACCCAGGT 
GTTCCAGGAG ACAAGGGATT CGGAGGTGTC CCAGGATTAC CAGGAATTCC AGGACCAAAG 
GGAGATGTCG GAAACCCAGG ACTCCCAGGT CTTAACGGAC AAAAGGGAGA ACCAGGAGTT 
GGAGTCCCAG GACAACCAGG ATCACCAGGA TTCCCAGGAC TTAAGGGAGA CGCCGGTCTT 
CCAGGTCTTC CAGGAACCCC AGGACTCGAG GGTCAACGTG GATTCCCAGG AGCCCCAGGA 
CTCAAGGGAG GAGACGGTCT TCCAGGACTG TCTGGTCAGC CAGGATACCC AGGAGAAAAG 
GGAGACGCCG GTTTGCCAGG AGTACCAGGG CGTGAAGGAT CGCCAGGATT CCCAGGTCAA 
GATGGACTTC CAGGAGTGCC AGGAATGAAG GGAGAAGACG GTCTTCCAGG ACTCCCAGGA 
GTTACTGGAC TCAAGGGAGA TCTCGGAGCA CCAGGACAAT CCGGAGCACC AGGACTCCCA 
GGAGCACCAG GATACCCAGG AATGAAGGGA AATGCGGGTA TCCCAGGAGT GCCAGGATTC 
AAGGGAGACG GAGGACTCCC AGGACTGCCA GGACTTAACG GACCAAAGGG AGAACCAGGA 
GTACCAGGAA TGCCAGGAAC CCCAGGAATG AAGGGTAACG GTGGTCTGCC AGGATTGCCA 
GGACGTGATG GACTTTCCGG AGTACCAGGA ATGAAGGGAG ATCGTGGTTT CAACGGACTT 
CCAGGAGAGA AGGGAGAAGC TGGACCAGCT GCTCGTGACG GACAAAAGGG AGATGCCGGT 
CTTCCAGGAC AGCCAGGTCT TCGTGGACCA CAAGGACCAT CTGGACTTCC AGGTGTCCCA 
GGATTCAAGG GAGAGACTGG ACTTCCAGGA TATGGACAAC CAGGTCAACC AGGAGAGAAG 
GGACTTCCTG GCATTCCAGG AAAGGCTGGA CGTCAAGGAG CCCCAGGGTC ACCAGGACAA 
GACGGACTCC CAGGATTCCC AGGAATGAAG GGAGAATCTG GATACCCAGG ACAAGACGGC 
CTTCCAGGGC GTGATGGCCT CCCAGGAGTC CCAGGACAAA AGGGAGATCT TGGACAGTCT 
GGACAGCCAG GACTCTCCGG GGCCCCAGGA CTCGACGGTC AACCAGGAGT ACCAGGAATC 
CGTGGAGACA AGGGACAAGG AGGACTACCC GGAATCCCAG GAGATCGTGG AATGGATGGT 
TATCCAGGAC AAAAGGGAGA GAACGGTTAC CCAGGTCAGC CAGGTCTTCC AGGACTTGGT 
GGAGAGAAAG GATTCGCTGG AACCCCAGGT TTCCCAGGAC TTAAGGGATC CCCAGGATAC 
CCAGGACAAG ACGGACTTCC AGGAATCCCT GGATTGAAGG GAGACTCAGG ATTCCCAGGA 
CAGCCAGGAC AAGAAGGACT CCCAGGACTT TCCGGAGAGA AGGGAATGGG TGGACTCCCA 
GGAATGCCAG GACAACCAGG TCAATCTATT GCTGGACCAG TTGGACCACC AGGTGCCCCA 
GGACTTCAAG GAAAAGACGG CTTCCCAGGC CTTCCAGGAC AAAAGGGAGA ATCCGGTCTT 
TCAGGCCTTC CAGGAGCCCC TGGATTGAAG GGAGAATCCG GCATGCCAGG ATTCCCAGGA 
GCTAAGGGAG ATCTAGGAGC CAATGGAATT CCAGGAAAGC GCGGAGAAGA CGGTCTACCA 
GGAGTACCAG GAAGAGATGG ACAACCAGGA ATCCCAGGAT TGAAGGGAGA AGTTGGAGGT 
GCTGGTCTTC CAGGACAACC AGGCTTCCCA GGTATCCCAG GACTTAAGGG AGAAGGAGGC 
CTTCCAGGTT TCCCAGGAGC AAAGGGAGAA GCTGGATTCC CAGGTACCCC AGGAGTGCCA 
GGATACGCCG GAGAGAAGGG AGACGGAGGT CTTCCAGGTC TTCCAGGAAG AGACGGACTC 
CCAGGAGCTG ATGGACCAGT GGGACCACCA GGACCATCAG GACCACAAAA CCTCGTTGAG 
CCGGGAGAGA AGGGACTTCC AGGTCTTCCA GGAGCCCCAG GACTTCGTGG AGAGAAGGGA 
ATGCCAGGGC TCGATGGACC ACCAGGAAAC GATGGACCAC CAGGTCTTCC AGGACAACGT 
GGAAACGACG GATACCCAGG AGCCCCAGGA TTATCAGGAG AGAAGGGAAT GGGAGGTCTT 
CCAGGTTTCC CAGGACTTGA CGGACAACCA GGAGGCCCAG GAGCCCCAGG ATTGCCAGGA 
GCACCAGGAG CTGCAGGACC AGCTTACAGA GACGGATTCG TTCTCGTCAA GCACTCCCAA 
ACCACCGAAG TACCAAGATG CCCAGAGGGT CAGACCAAGC TGTGGGACGG ATATTCCTTG 
TTGTACATTG AAGGAAACGA GAAATCCCAT AACCAAGACC TCGGACACGC TGGATCATGC 
CTCCAAAGAT TCTCCACCAT GCCATTCCTG TTCTGCGACT TCAACAACGT CTGCAACTAC 
GCCTCCAGAA ACGACAAGTC TTACTGGCTC TCAACATCTG AAGCTATCCC AATGATGCCA 
GTCAACGAGC GTGAAATCGA GCCATACATC TCGCGCTGCG CTGTGTGCGA AGCCCCAGCC 
AACACGATCG CTGTTCACTC CCAGACCATC CAAATTCCAA ACTGCCCAGC AGGATGGAGC 
AGCTTGTGGA TCGGATACTC GTTCGCCATG CACACCGGAG CCGGAGCCGA GGGAGGTGGT 
CAATCACTCT CCTCACCAGG ATCCTGCTTG GAAGACTTCC GTGCCACTCC ATTCATCGAG 
TGCAACGGTG CCCGCGGATC TTGCCACTAC TTTGCCAACA AGTTCTCATT CTGGCTTACC 
ACCATCGACA ATGACTCAGA ATTCAAGGTG CCAGAAAGTC AGACACTCAA GTCAGGAAAT 
CTTCGTACCC GCGTCTCAAG ATGCCAAGTT TGCGTCAAGT CCACCGACGG ACGTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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let-2 cDNA ORF clone, Caenorhabditis elegans

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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