DHCR24 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	DHCR24
Entrez Gene ID	106886480
Full Name	24-dehydrocholesterol reductase
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014939476.1	XP_014794962.1	delta(24)-sterol reductase

Pathways from BioSystems
Gene Ontology

The following DHCR24 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DHCR24 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa63212	XM_014939476.1 Latest version!	Calidris pugnax 24-dehydrocholesterol reductase (DHCR24), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DHCR24 ( XM_014939476.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014794962.1 Calidris pugnax 24-dehydrocholesterol reductase (DHCR24), mRNA.

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CloneID	OCa63212
Clone ID Related Accession (Same CDS sequence)	XM_014939476.1
Accession Version	XM_014939476.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	delta(24)-sterol reductase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090812.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCACCG GCCGGCCTGG CTGGTTGACT GTGTCACTTC GTGTTGGGAA GTATAAGAAG 
ATTCATAAGA ACATCATGAT CAACTTAATG GATGTTCTGG AAGTGGACAG TGAAAGACAG 
GTTGTTCGTG TGGAACCTTT GGTCACCATG GGCCAGTTGA CTGCACACCT GAATCCCATG 
GGCTGGACTA TTCCTGTGGT ACCAGAGCTC GATGATCTTA CAGTAGGTGG CCTGATCATG 
GGAACTGGCA TCGAGTCTTC GTCCCATATC TATGGGCTTT TTCAACATAC CTGCGTGGCC 
TATGAACTTG TTCTTGCCGA CGGAAGCCTT GTGAGATGTT CACCAACTGA AAATTCAGAC 
CTATTTTATG CAGTGCCTTG GTCTTGTGGT ACTCTGGGTT TCCTGGTTGC AGCAGAAATA 
AAAATGATTC CTGCCAAGAA ATATGTCAAA ATACATTACG AGCCTGTGAG AGGACTGCAG 
AAGATTTGTG AAAAGTTCAC AGAAGAATCG AAGAAAAAGG AGAATAGTTT TGTGGAAGGA 
CTTGTGTATT CTCTGGAAGA AGCAGTCATC ATGACAGGAG TCTTGACTGA TGAAGCAGAG 
CAAAGCAAGA TAAACAGAAT TGGCAACTAC TACAAGCCGT GGTTCTTTAA GCATGTGGAG 
AAGTATTTGA AAGCTGACAG GACTGGAGTA GAATACATTC CTGCAAGACA TTATTACCAT 
AGACATACAC GCAGTATCTT CTGGGAGCTC CAAGATATAA TTCCTTTTGG CAATAACCCC 
ATTTTCCGCT ACCTGTTTGG CTGGATGGTT CCTCCAAAAA TCTCTCTGTT AAAACTCACC 
CAAGGAGAAG CCATTCGGAA GCTGTACGAG CAACACCATG TTGTACAGGA CATGTTGGTC 
CCAATGAAGA GCCTTGAGAA GTCTATCCAA ACCTTCCATG TTGACCTTAA TGTGTACCCT 
CTTTGGTTAT GTCCTTTCAT ATTGCCCAAT AATCCTGGTA TGGTCCACCC CAAAGGGGAT 
GAAGCAGAAC TCTACGTGGA TATAGGAGCT TATGGAGAGC CCAAAAGGAA ACAATTTGAA 
GCCAGGGCTT CAATGAGGCA AATGGAAAAG TTTGTAAGAA GCGTGCATGG TTTCCAGATG 
CTGTATGCAG ATTGCTACAT GACCCGTGAG GAGTTCTGGG ATATGTTTGA TGGTTCGTTA 
TACCACAAGC TGAGGGAGCA GATGAATTGC AAGGATGCCT TTCCAGAAGT GTATGACAAA 
ATCTGCAAAG CTGCGAGGCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014794962.1
CDS	35..1318
Translation	MCTGRPGWLTVSLRVGKYKKIHKNIMINLMDVLEVDSERQVVRVEPLVTMGQLTAHLNPMGWTIPVVPELDDLTVGGLIMGTGIESSSHIYGLFQHTCVAYELVLADGSLVRCSPTENSDLFYAVPWSCGTLGFLVAAEIKMIPAKKYVKIHYEPVRGLQKICEKFTEESKKKENSFVEGLVYSLEEAVIMTGVLTDEAEQSKINRIGNYYKPWFFKHVEKYLKADRTGVEYIPARHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLKLTQGEAIRKLYEQHHVVQDMLVPMKSLEKSIQTFHVDLNVYPLWLCPFILPNNPGMVHPKGDEAELYVDIGAYGEPKRKQFEARASMRQMEKFVRSVHGFQMLYADCYMTREEFWDMFDGSLYHKLREQMNCKDAFPEVYDKICKAARH

Target ORF information:

RefSeq Version	XM_014939476.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax 24-dehydrocholesterol reductase (DHCR24), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014939476.1

ORF Insert Sequence:

ATGTGCACCG GCCGGCCTGG CTGGTTGACT GTGTCACTTC GTGTTGGGAA GTATAAGAAG 
ATTCATAAGA ACATCATGAT CAACTTAATG GATGTTCTGG AAGTGGACAG TGAAAGACAG 
GTTGTTCGTG TGGAACCTTT GGTCACCATG GGCCAGTTGA CTGCACACCT GAATCCCATG 
GGCTGGACTA TTCCTGTGGT ACCAGAGCTC GATGATCTTA CAGTAGGTGG CCTGATCATG 
GGAACTGGCA TCGAGTCTTC GTCCCATATC TATGGGCTTT TTCAACATAC CTGCGTGGCC 
TATGAACTTG TTCTTGCCGA CGGAAGCCTT GTGAGATGTT CACCAACTGA AAATTCAGAC 
CTATTTTATG CAGTGCCTTG GTCTTGTGGT ACTCTGGGTT TCCTGGTTGC AGCAGAAATA 
AAAATGATTC CTGCCAAGAA ATATGTCAAA ATACATTACG AGCCTGTGAG AGGACTGCAG 
AAGATTTGTG AAAAGTTCAC AGAAGAATCG AAGAAAAAGG AGAATAGTTT TGTGGAAGGA 
CTTGTGTATT CTCTGGAAGA AGCAGTCATC ATGACAGGAG TCTTGACTGA TGAAGCAGAG 
CAAAGCAAGA TAAACAGAAT TGGCAACTAC TACAAGCCGT GGTTCTTTAA GCATGTGGAG 
AAGTATTTGA AAGCTGACAG GACTGGAGTA GAATACATTC CTGCAAGACA TTATTACCAT 
AGACATACAC GCAGTATCTT CTGGGAGCTC CAAGATATAA TTCCTTTTGG CAATAACCCC 
ATTTTCCGCT ACCTGTTTGG CTGGATGGTT CCTCCAAAAA TCTCTCTGTT AAAACTCACC 
CAAGGAGAAG CCATTCGGAA GCTGTACGAG CAACACCATG TTGTACAGGA CATGTTGGTC 
CCAATGAAGA GCCTTGAGAA GTCTATCCAA ACCTTCCATG TTGACCTTAA TGTGTACCCT 
CTTTGGTTAT GTCCTTTCAT ATTGCCCAAT AATCCTGGTA TGGTCCACCC CAAAGGGGAT 
GAAGCAGAAC TCTACGTGGA TATAGGAGCT TATGGAGAGC CCAAAAGGAA ACAATTTGAA 
GCCAGGGCTT CAATGAGGCA AATGGAAAAG TTTGTAAGAA GCGTGCATGG TTTCCAGATG 
CTGTATGCAG ATTGCTACAT GACCCGTGAG GAGTTCTGGG ATATGTTTGA TGGTTCGTTA 
TACCACAAGC TGAGGGAGCA GATGAATTGC AAGGATGCCT TTCCAGAAGT GTATGACAAA 
ATCTGCAAAG CTGCGAGGCA CTGA