CHRM5 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHRM5
Entrez Gene ID	106887882
Full Name	cholinergic receptor muscarinic 5
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014941647.1	XP_014797133.1	muscarinic acetylcholine receptor M5

Pathways from BioSystems
Gene Ontology

The following CHRM5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRM5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa64787	XM_014941647.1 Latest version!	Calidris pugnax cholinergic receptor, muscarinic 5 (CHRM5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHRM5 ( XM_014941647.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014797133.1 Calidris pugnax cholinergic receptor, muscarinic 5 (CHRM5), mRNA.

Price & Availability↑

CloneID	OCa64787
Clone ID Related Accession (Same CDS sequence)	XM_014941647.1
Accession Version	XM_014941647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	muscarinic acetylcholine receptor M5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090839.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTAA ATTTATTCAG CAATTCCACT GTTGTAAACA GTTCATCCAT CAACCATAAG 
CAGTTAGAAG GGCATAGCCT CTGGGAAGTC ATTACTATTG CTACTGTAAC AGCAATTGTA 
AGCTTAATAA CCATAGTGGG AAATATTCTT GTAATGATAT CCTTCAAGGT TAACAGTCAG 
CTCAAAACTG TCAACAATTA TTACTTGCTC AGCCTCGCCT GTGCAGATCT CATCATTGGA 
ATATTTTCCA TGAACCTTTA TACATCTTAT ATACTCATAG GCCACTGGTC TCTTGGAAGC 
CTTGCCTGTG ACCTGTGGCT AGCACTGGAC TATGTAGCTA GTAATGCCTC AGTAATGAAC 
CTCCTAGTCA TCAGTTTTGA CAGATATTTT TCCATCACAA GGCCTTTAAC GTACAGGGCC 
AAGCGCACAC CTAAAAGAGC TGGAATCATG ATTGGCCTGG CCTGGCTAAT TTCCTTCGTG 
TTGTGGGCAC CTGTAATCTT GTGCTGGCAG TATTTTGTGG GTGAACGAAC AGTACCACCT 
GAGGAGTGCC AGATACAGTT TTTATACGAG CCCATTGTCA CCTTTGGTAC TGCAATTGCT 
GCTTTTTACA TTCCAGTGTC CGTGATGACC ATTCTGTATT GCCGCATCTA TAAAGAGACA 
GAGAAACGTA CCAAGGACCT TGCTGAACTG CAGGGTTCAG AGTCTGTGGC AGAGTTTGAG 
ATGATAAAGC CTCAGAAAGC TTTCCTGAAG TCTTGCTTCA GTTGCAAGCA GCAAAACGTA 
ACCAAAAGAG AGAGGTGTCA AGCTTCCTGG TCTTCATCTA GTCGAAGTAC GTCAGCTACG 
GTGAAAGCCT CTCAGGCAAC AAGTACTTGT AACGACTGGG CTAAGGCTGA CCAGTTAACC 
ACCTGCAGCA GCTATGCCTC TTCAGAAGAG GAGGATAAAC TCACTGCTGA TTCCGTTTTC 
CAAGTAACTT ACAAAAGTCC ATCTAAAGGT AAGGCAGAAG AGTTTAATGA CAGTACAGAT 
GTTGTTGTCA AAGACCAACC TGAAGAAAAT GATTTTGAGA ATCAGAAATA CTTTTTGTCT 
CCTGCCAAAG GCCACGTACA AAAAAGTAAA AAATGTGTGG CCTATAAATT CCGGTTGGTG 
GTTAAGGCTG ATGGCAGCCA AGAAGCCAAC AATGGTTGCC GTAAAGTAAA AATAACTCCT 
TGTTCTGCTG CTCTGTCGAA GGATCCTTCC ATCAAAAGCA TGGATCCAAA TATAAATAAC 
CAAATAACCA AAAGGAAACG GATGGTTCTT ATAAAGGAAC GCAAAGCAGC ACAGACTTTA 
AGTGCCATTC TTTTGGCTTT TATCATCACA TGGACTCCCT ACAATATCAT GGTTTTGATC 
TCCACATTCT GCTCTGACTG CATTCCCCTG ACACTGTGGC ACCTTGGATA TTGGCTATGC 
TATGTGAACA GCACTGTTAA CCCCATGTGT TATGCCCTCT GTAACAAAAC TTTCAGGAAA 
ACTTTTAAGA TGCTGCTTTT CTGCCAGTGG AAAAAGAAAA AAGTGGAAGA AAAACTGTAC 
TGGCAGGGCA ATACCAGACT GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014797133.1
CDS	976..2562
Translation	MEVNLFSNSTVVNSSSINHKQLEGHSLWEVITIATVTAIVSLITIVGNILVMISFKVNSQLKTVNNYYLLSLACADLIIGIFSMNLYTSYILIGHWSLGSLACDLWLALDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTPKRAGIMIGLAWLISFVLWAPVILCWQYFVGERTVPPEECQIQFLYEPIVTFGTAIAAFYIPVSVMTILYCRIYKETEKRTKDLAELQGSESVAEFEMIKPQKAFLKSCFSCKQQNVTKRERCQASWSSSSRSTSATVKASQATSTCNDWAKADQLTTCSSYASSEEEDKLTADSVFQVTYKSPSKGKAEEFNDSTDVVVKDQPEENDFENQKYFLSPAKGHVQKSKKCVAYKFRLVVKADGSQEANNGCRKVKITPCSAALSKDPSIKSMDPNINNQITKRKRMVLIKERKAAQTLSAILLAFIITWTPYNIMVLISTFCSDCIPLTLWHLGYWLCYVNSTVNPMCYALCNKTFRKTFKMLLFCQWKKKKVEEKLYWQGNTRLP

Target ORF information:

RefSeq Version	XM_014941647.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax cholinergic receptor, muscarinic 5 (CHRM5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014941647.1

ORF Insert Sequence:

ATGGAAGTAA ATTTATTCAG CAATTCCACT GTTGTAAACA GTTCATCCAT CAACCATAAG 
CAGTTAGAAG GGCATAGCCT CTGGGAAGTC ATTACTATTG CTACTGTAAC AGCAATTGTA 
AGCTTAATAA CCATAGTGGG AAATATTCTT GTAATGATAT CCTTCAAGGT TAACAGTCAG 
CTCAAAACTG TCAACAATTA TTACTTGCTC AGCCTCGCCT GTGCAGATCT CATCATTGGA 
ATATTTTCCA TGAACCTTTA TACATCTTAT ATACTCATAG GCCACTGGTC TCTTGGAAGC 
CTTGCCTGTG ACCTGTGGCT AGCACTGGAC TATGTAGCTA GTAATGCCTC AGTAATGAAC 
CTCCTAGTCA TCAGTTTTGA CAGATATTTT TCCATCACAA GGCCTTTAAC GTACAGGGCC 
AAGCGCACAC CTAAAAGAGC TGGAATCATG ATTGGCCTGG CCTGGCTAAT TTCCTTCGTG 
TTGTGGGCAC CTGTAATCTT GTGCTGGCAG TATTTTGTGG GTGAACGAAC AGTACCACCT 
GAGGAGTGCC AGATACAGTT TTTATACGAG CCCATTGTCA CCTTTGGTAC TGCAATTGCT 
GCTTTTTACA TTCCAGTGTC CGTGATGACC ATTCTGTATT GCCGCATCTA TAAAGAGACA 
GAGAAACGTA CCAAGGACCT TGCTGAACTG CAGGGTTCAG AGTCTGTGGC AGAGTTTGAG 
ATGATAAAGC CTCAGAAAGC TTTCCTGAAG TCTTGCTTCA GTTGCAAGCA GCAAAACGTA 
ACCAAAAGAG AGAGGTGTCA AGCTTCCTGG TCTTCATCTA GTCGAAGTAC GTCAGCTACG 
GTGAAAGCCT CTCAGGCAAC AAGTACTTGT AACGACTGGG CTAAGGCTGA CCAGTTAACC 
ACCTGCAGCA GCTATGCCTC TTCAGAAGAG GAGGATAAAC TCACTGCTGA TTCCGTTTTC 
CAAGTAACTT ACAAAAGTCC ATCTAAAGGT AAGGCAGAAG AGTTTAATGA CAGTACAGAT 
GTTGTTGTCA AAGACCAACC TGAAGAAAAT GATTTTGAGA ATCAGAAATA CTTTTTGTCT 
CCTGCCAAAG GCCACGTACA AAAAAGTAAA AAATGTGTGG CCTATAAATT CCGGTTGGTG 
GTTAAGGCTG ATGGCAGCCA AGAAGCCAAC AATGGTTGCC GTAAAGTAAA AATAACTCCT 
TGTTCTGCTG CTCTGTCGAA GGATCCTTCC ATCAAAAGCA TGGATCCAAA TATAAATAAC 
CAAATAACCA AAAGGAAACG GATGGTTCTT ATAAAGGAAC GCAAAGCAGC ACAGACTTTA 
AGTGCCATTC TTTTGGCTTT TATCATCACA TGGACTCCCT ACAATATCAT GGTTTTGATC 
TCCACATTCT GCTCTGACTG CATTCCCCTG ACACTGTGGC ACCTTGGATA TTGGCTATGC 
TATGTGAACA GCACTGTTAA CCCCATGTGT TATGCCCTCT GTAACAAAAC TTTCAGGAAA 
ACTTTTAAGA TGCTGCTTTT CTGCCAGTGG AAAAAGAAAA AAGTGGAAGA AAAACTGTAC 
TGGCAGGGCA ATACCAGACT GCCATAA