SPIRE2 cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPIRE2
Entrez Gene ID	106888179
Full Name	spire type actin nucleation factor 2
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014942211.1	XP_014797697.1	protein spire homolog 2

Pathways from BioSystems
Gene Ontology

The following SPIRE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPIRE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa65204	XM_014942211.1 Latest version!	Calidris pugnax spire-type actin nucleation factor 2 (SPIRE2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPIRE2 ( XM_014942211.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014797697.1 Calidris pugnax spire-type actin nucleation factor 2 (SPIRE2), mRNA.

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CloneID	OCa65204
Clone ID Related Accession (Same CDS sequence)	XM_014942211.1
Accession Version	XM_014942211.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	protein spire homolog 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015090842.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCCAC TGCTGACGCC CTCTGCTGAA GCCCAGATGG TGCAGTCGTT GGGCTTTGCC 
ATCTACCGGG CACTGGATTG GGGACTGGAC GAGAACGAGG AGCGAGAGCT GAGCCCACGG 
CTGGAGCAGC TCATCGACCT GATGACCAAC AGCGACTCAG AGGACAGTGG CTGCGGCACG 
GCCGACGAGG GCTATGGGGG ACAGGAGGAG GAGGAAGAGG GGGGCGAGGG GCCACCACGC 
TCCGTGCGCA CCTTCGGGCA GGCCATGCGG TGTTGTGCCG CCCGCCTGGC TGACCCCGCC 
GAAGCCCAGG CTCACTACCA GGCTGTCTGC CGGGCGCTCT TCGCCGAGAC CGTGGAGCTG 
AAAGCCTTCC TCGCCAAGAT CCGTGATGCC AAGGAGATGC TGCAGAAGCT GAAGGAGGAT 
GAGGAAGCAG AGGAGCGGCC GGCGGCGGAG CTGGGCAGCC TGCGCAACAC CGACTGGGCC 
CGGCTGTGGG TGCAGCTGAT GCGGGAGCTG CGGCATGGTG TGAAGCTGAA GAAGGTGCAG 
GAAAAGCAGT TCAACCCCTT GCCCACCGAG TACCAGCTCA CCCCCTTCGA GATGCTCATG 
CAGGACATCC GTGCCCGCAA CTACAAACTC CGCAAGGTCA TGGTGGATGG AGACATCCCC 
CCCCGGGTGA AGAAGAATGC CCACGAGCTC ATCCTCGACT TCATCCGCTC CCGGCCCCCA 
CTGAAGCAGG CATCGGAGCG GCAACTGCGG CCCCTACCCC AGAAGCAGAG GACGCTCCAT 
GAGAAGATCC TTGAGGAGAT CAGGCAGGAG AGGAAGCTAC GGCCTGTGGA GATGCCATAC 
CGTGGCCAGA AAGGCTACAG CTCCCTGCCC TGCATCCCCC ATGCCTGTGC GGGTCACCTG 
GCTTCCAGCT CCTGCCTCGA GCTCTCCCGG ACCCCAGCGA CCACCGTGCC AGCACACCCA 
CGGCCCCGCG TCCTCCTCAA GGCACCCACC CTGGCTGAGA TGGAGGAGAT GAACGTCTCT 
GAGGAGGAAG ACTCTCCGAT CGCAGAGGTG GCAATGAAGC GGGACCGCTC CTTCTCAGAA 
CATGACCTGG CGCAGCTGCA GAGCCAGCTG GGGGGCAGTG AACCTGCACC CCAGGAGCTG 
GAGTCACTGC AGCCCGAGCC CCGGCCCCGC TCAGGCTCCG TCCCTGCCAG CTGCCACCCG 
TGGCCAGACA GCTCGGCACC ACCCCGGGCT GCCCTTGGTG CCGTGGAGGA GAGGCCAGAG 
GATGGATCCA GCACTGCCCC TGGCACCAGC TCCAAGCACC TCTGGCTGGA GTTCAGCCAT 
CCCGTGGAGA GCCTGGCCCT CACCGTGGAG GAGATGATCA ACGTCCGCAG GGTGCTGGTC 
AAGGCAGAGA TGGAGAAGTT CCTGCAGAGC AAGGAGCTGT ACAGCAGCCT GCGGAAGGGG 
AAGGTCTGCT GCTGCTGCAG GACCAAGTTC CCCCTCTTCT CCTGGCCCGC AGCTTGCCTC 
TTCTGCAAGC GGTCTGTCTG CAGCTCCTGC AGCCTGAAGA TGAAGATGCC TTCCAAGAAG 
CTGGCTCACA TCCCCGTCTA CGCACTGGGC TTCGAGAGCC TGCCGGGCTC GCTGCTGGCC 
AAAGCCCTGC CGCTGCGGAG GAGAGAGACC TTCCACTCAC TCCAGGGCAT GTGCTGGCGC 
CGGGTGGAAG AGGAGTTCCC CCACATCTAC GCCCAGGGCT CTGTCCTGCG CGACGTCTGC 
AGCGACTGCG CCGGCTTCGT CACCGACGTC ATCTGCTCCA GCCGCCGCAG CGTGGCCGTC 
CTCAACGCCG CCCCGCGCCG CGCCAAGGCC CGCTCCCTCT ACTGCGAATC CTGGCACAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014797697.1
CDS	100..1962
Translation	MPPLLTPSAEAQMVQSLGFAIYRALDWGLDENEERELSPRLEQLIDLMTNSDSEDSGCGTADEGYGGQEEEEEGGEGPPRSVRTFGQAMRCCAARLADPAEAQAHYQAVCRALFAETVELKAFLAKIRDAKEMLQKLKEDEEAEERPAAELGSLRNTDWARLWVQLMRELRHGVKLKKVQEKQFNPLPTEYQLTPFEMLMQDIRARNYKLRKVMVDGDIPPRVKKNAHELILDFIRSRPPLKQASERQLRPLPQKQRTLHEKILEEIRQERKLRPVEMPYRGQKGYSSLPCIPHACAGHLASSSCLELSRTPATTVPAHPRPRVLLKAPTLAEMEEMNVSEEEDSPIAEVAMKRDRSFSEHDLAQLQSQLGGSEPAPQELESLQPEPRPRSGSVPASCHPWPDSSAPPRAALGAVEERPEDGSSTAPGTSSKHLWLEFSHPVESLALTVEEMINVRRVLVKAEMEKFLQSKELYSSLRKGKVCCCCRTKFPLFSWPAACLFCKRSVCSSCSLKMKMPSKKLAHIPVYALGFESLPGSLLAKALPLRRRETFHSLQGMCWRRVEEEFPHIYAQGSVLRDVCSDCAGFVTDVICSSRRSVAVLNAAPRRAKARSLYCESWHK

Target ORF information:

RefSeq Version	XM_014942211.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax spire-type actin nucleation factor 2 (SPIRE2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014942211.1

ORF Insert Sequence:

ATGCCTCCAC TGCTGACGCC CTCTGCTGAA GCCCAGATGG TGCAGTCGTT GGGCTTTGCC 
ATCTACCGGG CACTGGATTG GGGACTGGAC GAGAACGAGG AGCGAGAGCT GAGCCCACGG 
CTGGAGCAGC TCATCGACCT GATGACCAAC AGCGACTCAG AGGACAGTGG CTGCGGCACG 
GCCGACGAGG GCTATGGGGG ACAGGAGGAG GAGGAAGAGG GGGGCGAGGG GCCACCACGC 
TCCGTGCGCA CCTTCGGGCA GGCCATGCGG TGTTGTGCCG CCCGCCTGGC TGACCCCGCC 
GAAGCCCAGG CTCACTACCA GGCTGTCTGC CGGGCGCTCT TCGCCGAGAC CGTGGAGCTG 
AAAGCCTTCC TCGCCAAGAT CCGTGATGCC AAGGAGATGC TGCAGAAGCT GAAGGAGGAT 
GAGGAAGCAG AGGAGCGGCC GGCGGCGGAG CTGGGCAGCC TGCGCAACAC CGACTGGGCC 
CGGCTGTGGG TGCAGCTGAT GCGGGAGCTG CGGCATGGTG TGAAGCTGAA GAAGGTGCAG 
GAAAAGCAGT TCAACCCCTT GCCCACCGAG TACCAGCTCA CCCCCTTCGA GATGCTCATG 
CAGGACATCC GTGCCCGCAA CTACAAACTC CGCAAGGTCA TGGTGGATGG AGACATCCCC 
CCCCGGGTGA AGAAGAATGC CCACGAGCTC ATCCTCGACT TCATCCGCTC CCGGCCCCCA 
CTGAAGCAGG CATCGGAGCG GCAACTGCGG CCCCTACCCC AGAAGCAGAG GACGCTCCAT 
GAGAAGATCC TTGAGGAGAT CAGGCAGGAG AGGAAGCTAC GGCCTGTGGA GATGCCATAC 
CGTGGCCAGA AAGGCTACAG CTCCCTGCCC TGCATCCCCC ATGCCTGTGC GGGTCACCTG 
GCTTCCAGCT CCTGCCTCGA GCTCTCCCGG ACCCCAGCGA CCACCGTGCC AGCACACCCA 
CGGCCCCGCG TCCTCCTCAA GGCACCCACC CTGGCTGAGA TGGAGGAGAT GAACGTCTCT 
GAGGAGGAAG ACTCTCCGAT CGCAGAGGTG GCAATGAAGC GGGACCGCTC CTTCTCAGAA 
CATGACCTGG CGCAGCTGCA GAGCCAGCTG GGGGGCAGTG AACCTGCACC CCAGGAGCTG 
GAGTCACTGC AGCCCGAGCC CCGGCCCCGC TCAGGCTCCG TCCCTGCCAG CTGCCACCCG 
TGGCCAGACA GCTCGGCACC ACCCCGGGCT GCCCTTGGTG CCGTGGAGGA GAGGCCAGAG 
GATGGATCCA GCACTGCCCC TGGCACCAGC TCCAAGCACC TCTGGCTGGA GTTCAGCCAT 
CCCGTGGAGA GCCTGGCCCT CACCGTGGAG GAGATGATCA ACGTCCGCAG GGTGCTGGTC 
AAGGCAGAGA TGGAGAAGTT CCTGCAGAGC AAGGAGCTGT ACAGCAGCCT GCGGAAGGGG 
AAGGTCTGCT GCTGCTGCAG GACCAAGTTC CCCCTCTTCT CCTGGCCCGC AGCTTGCCTC 
TTCTGCAAGC GGTCTGTCTG CAGCTCCTGC AGCCTGAAGA TGAAGATGCC TTCCAAGAAG 
CTGGCTCACA TCCCCGTCTA CGCACTGGGC TTCGAGAGCC TGCCGGGCTC GCTGCTGGCC 
AAAGCCCTGC CGCTGCGGAG GAGAGAGACC TTCCACTCAC TCCAGGGCAT GTGCTGGCGC 
CGGGTGGAAG AGGAGTTCCC CCACATCTAC GCCCAGGGCT CTGTCCTGCG CGACGTCTGC 
AGCGACTGCG CCGGCTTCGT CACCGACGTC ATCTGCTCCA GCCGCCGCAG CGTGGCCGTC 
CTCAACGCCG CCCCGCGCCG CGCCAAGGCC CGCTCCCTCT ACTGCGAATC CTGGCACAAG 
TGA